CN108523133A - 一种益生菌复合微粒及其制备方法 - Google Patents
一种益生菌复合微粒及其制备方法 Download PDFInfo
- Publication number
- CN108523133A CN108523133A CN201810266044.7A CN201810266044A CN108523133A CN 108523133 A CN108523133 A CN 108523133A CN 201810266044 A CN201810266044 A CN 201810266044A CN 108523133 A CN108523133 A CN 108523133A
- Authority
- CN
- China
- Prior art keywords
- probiotics
- composite particles
- tannalbin
- oligofructose
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 54
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 54
- 239000011246 composite particle Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims description 10
- 206010012735 Diarrhoea Diseases 0.000 claims abstract description 12
- 210000004347 intestinal mucosa Anatomy 0.000 claims abstract description 7
- 238000011282 treatment Methods 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims description 2
- 241000186000 Bifidobacterium Species 0.000 claims description 2
- 239000001263 FEMA 3042 Substances 0.000 claims description 2
- 244000199866 Lactobacillus casei Species 0.000 claims description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 2
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 2
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims description 2
- 229940033123 tannic acid Drugs 0.000 claims description 2
- 235000015523 tannic acid Nutrition 0.000 claims description 2
- 229920002258 tannic acid Polymers 0.000 claims description 2
- 229930091371 Fructose Natural products 0.000 claims 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 229960002737 fructose Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000002496 gastric effect Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000004567 concrete Substances 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 206010004016 Bacterial diarrhoea Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000005577 Gastroenteritis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 208000012873 acute gastroenteritis Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 229940125714 antidiarrheal agent Drugs 0.000 description 1
- 239000003793 antidiarrheal agent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003613 bile acid Substances 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000001507 sample dispersion Methods 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/121—Brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开了一种益生菌复合微粒，所述复合微粒包含益生菌、鞣酸蛋白及低聚果糖。本发明所公开的益生菌复合微粒，成分工艺简单，添加少、应用于治疗腹泻及肠粘膜修复低剂量即有极好的效果，并可在常温下较长期存放。
Description
技术领域
本发明涉及一种益生菌复合微粒及其制备方法。
背景技术
研究表明，益生菌对机体有重要的生理功能，包括整肠作用，维持肠道菌群平衡，防治腹泻；缓解乳糖不耐症，促进机体营养吸收；增强免疫力；降低高血压；降低胆固醇；预防癌症，抑制肿瘤生长等等作用，因而益生菌产品已被广泛用于保健、医药等领域。但由于益生菌的耐受性较差，活菌制剂进入消化道后难以经受低pH值的胃酸、胆汁酸，消化酶等的作用，使之难以有足够的活菌数量到达肠道、进行增殖并发挥作用。
目前，对益生菌的保护方法主要是将益生菌用载体包埋，利用载体材料自身的传质阻力以阻止胃酸等与益生菌相接触而起到保护作用，提升了到达肠道的活菌数量，但存在壁材组成成分复杂，占比高，工艺复杂，效果不佳等问题，对于益生菌的应用存在一定的不利影响。
发明内容
本发明要解决的技术问题是提供一种成分工艺简单、添加少、效果好的益生菌复合微粒。
为解决上述技术问题，本发明公开了一种益生菌复合微粒，所述复合微粒包含益生菌、鞣酸蛋白及低聚果糖。
所述益生菌优选为鼠李糖乳杆菌、乳酸双歧杆菌或干酪乳杆菌，优选为冻干菌粉，活菌数1×1010～1×1013cfu/g。所述鞣酸蛋白与低聚果糖的重量比优选为1:2～4，更优选为1:3。
本发明所公开的益生菌复合微粒，可通过如下步骤制备：a)低聚果糖置于水中，溶解得澄清水溶液；b)步骤a)水溶液中加入鞣酸蛋白，混合均匀后除菌；c)步骤b)溶液中加入益生菌，均质后干燥。
本发明所公开的益生菌复合微粒适用于治疗腹泻及肠粘膜修复。
鞣酸蛋白由鸡蛋白的稀溶液与鞣酸作用制得，临床上常作为止泻药，适用于急性胃肠炎及各种非细菌性腹泻、小儿消化不良等，需较大剂量才能发挥作用(成人:每天3次，每次1～2g，空腹服)，并有便秘等副作用，本发明的研究人员意外发现，将益生菌、鞣酸蛋白及低聚果糖制备成复合微粒，可能是由于低聚果糖的传质阻力，及鞣酸蛋白在胃内不分解，及对胃蛋白酶、胰酶、乳酶生等消化酶类活性的抑制，使益生菌可以以较高的活性状态到达肠道顺利定殖，发挥作用，且与鞣酸蛋白的止泻有较好的协同作用，本发明所公开的益生菌复合微粒应用于治疗腹泻及肠粘膜修复，低剂量即有极好的效果，并可在常温下较长期存放。
具体实施方式
以下通过具体实施例再对本发明的上述内容作进一步的详细说明。但不应将此理解为本发明上述主题的范围仅局限于以下的实施例。在不脱离本发明上述技术思想的情况下，根据本领域普通技术知识和惯用手段做出的各种替换或变更，均应包括在本发明的范围内。
实施例1
处方：
| 物料组成 | 处方配比 |
| 益生菌 | 3.4×1010cfu |
| 鞣酸蛋白 | 1.0g |
| 低聚果糖 | 2.0g |
制备方法：
1)低聚果糖置于水中，溶解得澄清水溶液；
2)步骤1)水溶液中加入鞣酸蛋白，混合均匀后除菌；
3)步骤2)溶液中加入益生菌，均质后冷冻干燥。
实施例2
处方：
| 物料组成 | 处方配比 |
| 益生菌 | 5.4×1011cfu |
| 鞣酸蛋白 | 1.0g |
| 低聚果糖 | 4.0g |
制备方法：
1)低聚果糖置于水中，溶解得澄清水溶液；
2)步骤2)水溶液中加入鞣酸蛋白，混合均匀后除菌；
3)步骤3)溶液中加入益生菌，均质后冷冻干燥。
实施例3
处方：
| 物料组成 | 处方配比 |
| 益生菌 | 5.4×1011cfu |
| 鞣酸蛋白 | 1.0g |
| 低聚果糖 | 4.0g |
制备方法：
1)鞣酸蛋白粉碎至粒径D90约20μm，(使用Micros超细粉碎机粉碎，粒径应用马尔文2000激光粒度仪检测)
2)低聚果糖置于水中，溶解得澄清水溶液；
3)步骤2)水溶液中加入鞣酸蛋白，混合均匀后除菌；
4)步骤3)溶液中加入益生菌，均质后冷冻干燥。
实施例4
处方：
| 物料组成 | 处方配比 |
| 益生菌 | 5.4×1012cfu |
| 鞣酸蛋白 | 1.0g |
| 低聚果糖 | 3.0g |
制备方法：
1)鞣酸蛋白粉碎至粒径D90约10μm，(使用Micros超细粉碎机粉碎，粒径应用马尔文2000激光粒度仪检测)
2)低聚果糖置于水中，溶解得澄清水溶液；
3)步骤2)水溶液中加入鞣酸蛋白，混合均匀后除菌；
4)步骤3)溶液中加入益生菌，均质后冷冻干燥。
实施例5
处方：
| 物料组成 | 处方配比 |
| 益生菌 | 2.1×1013cfu |
| 鞣酸蛋白 | 1.0g |
| 低聚果糖 | 3.0g |
制备方法：
1)鞣酸蛋白粉碎至粒径D90约1μm，(使用Micros超细粉碎机粉碎，粒径应用马尔文2000激光粒度仪检测)
2)低聚果糖置于水中，溶解得澄清水溶液；
3)步骤2)水溶液中加入鞣酸蛋白，混合均匀后除菌；
4)步骤3)溶液中加入益生菌，均质后冷冻干燥。
本发明以上实施例所制备的益生菌复合微粒的试验测试结果如下：
1)以上实施例所得到的益生菌复合微粒为白色粉末，电子显微镜扫描结果显示各实施例样品分散均均匀，基元为直径约60-80微米的微球。
2)以上实施例制备的益生菌复合微粒各1g置于100mL的人工胃液中，人工胃液pH值为2.0，37℃厌氧处理，分别于0、0.5、1、2、3小时后收集复合微粒，并进行活菌计数，分析复合微粒对人工胃液的耐受情况，结果显示实施例制得的益生菌复合微粒在人工胃液中性质较稳定，可以较好的保护菌体免受胃酸的伤害，能以较高的活性状态到达肠道顺利定殖。具体结果见下表1：
表1
3)实施例所制备的益生菌复合微粒治疗腹泻及肠粘膜修复的疗效验证：采用出生后21天大鼠，制备刺激性腹泻模型，造模成功后，给予治疗，每组10只，阴性对照组给予生理盐水灌胃，阳性对照组给予鞣酸蛋白，实施例组给予益生菌复合微粒0.02g/kg(按鞣酸蛋白计)，分两次灌胃给予，连续5天。观察每日给药后5h内稀便率(动物排稀便次数/动物排便总次数×100％)，每日记录腹泻率(腹泻的动物数/该组动物总数×100％)，第6天处死实验动物，各取1.5cm左右长的空肠进行组织病理学研究，按chiu标准对小肠黏膜损伤进行分级。结果显示实施例制得的益生菌复合微粒对于腹泻及肠粘膜修复有良好的作用，疗效远优于同等剂量的鞣酸蛋白。具体结果见下表2
表2
4)实施例所制备的益生菌复合微粒,于25℃、60％RH条件下放置考察其稳定性，实施例1～5的稳定性皆较好，可长期存放至12个月以上。具体结果见下表3：
表3
Claims (9)
1.一种益生菌复合微粒，其特征在于：所述复合微粒包含益生菌、鞣酸蛋白及低聚果糖。
2.如权利要求1所述益生菌复合微粒，其特征在于：所述益生菌为鼠李糖乳杆菌、乳酸双歧杆菌或干酪乳杆菌。
3.如权利要求1所述益生菌复合微粒，其特征在于：所述鞣酸蛋白与低聚果糖的重量比为1:2～4。
4.如权利要求1所述益生菌复合微粒，其特征在于：所述鞣酸蛋白与低聚果糖的重量比为1:3。
5.如权利要求1所述益生菌复合微粒，其特征在于：所述益生菌为冻干菌粉。
6.如权利要求1所述益生菌复合微粒，其特征在于：所述益生菌的活菌数1×1010～1×1013cfu/g。
7.如权利要求1所述益生菌复合微粒的制备方法，其特征在于：包含如下步骤：
a)低聚果糖置于水中，溶解得澄清水溶液；
b)步骤a)水溶液中加入鞣酸蛋白，混合均匀后除菌；
c)步骤b)溶液中加入益生菌，均质后干燥。
8.如权利要求7所述的益生菌复合微粒的制备方法，其特征在于：所述步骤b)中鞣酸蛋白的粒径小于20μm。
9.如权利要求1所述的益生菌复合微粒在治疗腹泻及肠粘膜修复中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266044.7A CN108523133A (zh) | 2018-03-28 | 2018-03-28 | 一种益生菌复合微粒及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266044.7A CN108523133A (zh) | 2018-03-28 | 2018-03-28 | 一种益生菌复合微粒及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108523133A true CN108523133A (zh) | 2018-09-14 |
Family
ID=63481504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810266044.7A Withdrawn CN108523133A (zh) | 2018-03-28 | 2018-03-28 | 一种益生菌复合微粒及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108523133A (zh) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552871A (zh) * | 2011-12-13 | 2012-07-11 | 海南康芝药业股份有限公司 | 一种鞣酸蛋白酵母咀嚼片及其生产方法 |
| CN103876145A (zh) * | 2014-04-01 | 2014-06-25 | 吉林大学 | 一种益生菌微生态片剂及其制备方法 |
| CN104431354A (zh) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | 一种益生菌微胶囊及其制备方法和应用 |
| CN104497131A (zh) * | 2014-12-23 | 2015-04-08 | 青岛康原药业有限公司 | 一种用卵清蛋白制备鞣酸蛋白的方法 |
| CN106418547A (zh) * | 2016-10-09 | 2017-02-22 | 西安创客村电子商务有限责任公司 | 一种益生菌微胶囊及其制备方法 |
-
2018
- 2018-03-28 CN CN201810266044.7A patent/CN108523133A/zh not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552871A (zh) * | 2011-12-13 | 2012-07-11 | 海南康芝药业股份有限公司 | 一种鞣酸蛋白酵母咀嚼片及其生产方法 |
| CN103876145A (zh) * | 2014-04-01 | 2014-06-25 | 吉林大学 | 一种益生菌微生态片剂及其制备方法 |
| CN104431354A (zh) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | 一种益生菌微胶囊及其制备方法和应用 |
| CN104497131A (zh) * | 2014-12-23 | 2015-04-08 | 青岛康原药业有限公司 | 一种用卵清蛋白制备鞣酸蛋白的方法 |
| CN106418547A (zh) * | 2016-10-09 | 2017-02-22 | 西安创客村电子商务有限责任公司 | 一种益生菌微胶囊及其制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gu et al. | Encapsulation of multiple probiotics, synbiotics, or nutrabiotics for improved health effects: A review | |
| CN108991327A (zh) | 一种益生菌固体饮料及其制备方法 | |
| KR101370143B1 (ko) | 프로바이오틱 저장 및 전달 | |
| CN108743851A (zh) | 一种复合益生菌粉的制备方法及复合益生菌粉 | |
| CN1843385A (zh) | 肠道微生态制剂及其制备方法 | |
| CN112716982B (zh) | 含乳酸菌的组合物及其用途 | |
| CN101366734A (zh) | 合生素药物组合物 | |
| TW201834673A (zh) | 包含猴頭菇的益生菌組合物 | |
| CN108770974A (zh) | 一种抗过敏的益生菌凝胶糖果及其制备方法 | |
| CN110917174A (zh) | 调节女性阴道菌群平衡的口服益生菌胶囊剂及其制备方法 | |
| CN108783169A (zh) | 一种四联益生菌固体饮料及其制备方法 | |
| CN116121154B (zh) | 一种乳明串珠菌及其应用 | |
| CN106798755B (zh) | 一种具有调理乳腺增生功能的益生菌组合物及其应用 | |
| CN113230284B (zh) | 一种基于多维交联的合生素微囊化制剂及其制备方法和应用 | |
| CN113876935A (zh) | 一种治疗宠物肠道疾病的组合物及其应用 | |
| WO2022001673A1 (zh) | N-乙酰-d-氨基葡萄糖的新用途及相关产品 | |
| WO2021152869A1 (ja) | 腸溶性組成物、該腸溶性組成物を含む飲食品、該腸溶性組成物の崩壊時間を制御する方法、及び該腸溶性組成物の製造方法 | |
| CN108523133A (zh) | 一种益生菌复合微粒及其制备方法 | |
| CN109645473A (zh) | 一种改善胃肠道和增强免疫力的组合物及其应用 | |
| CN107836721A (zh) | 一种具有胃肠道保健功能的组合物及其制备方法 | |
| CN111990648A (zh) | 一种护胃养胃的益生菌粉及其制备方法 | |
| KR20180016164A (ko) | 발효 혼합 식이섬유 유산균 제조방법 | |
| TW202140049A (zh) | 用於治療ｂ型肝炎（ｈｂｖ）及ｄ型肝炎（ｈｄｖ）之組合物及方法 | |
| CN111567809A (zh) | 具有提高免疫力和调节肠道功能的动物双歧杆菌bz11复合菌剂 | |
| CN113730546B (zh) | 一种治疗帕金森病的药物组合物及其用途 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180914 |
|
| WW01 | Invention patent application withdrawn after publication |