CN108522173A - A kind of screening technique of shade tolerant corn inbred line - Google Patents
A kind of screening technique of shade tolerant corn inbred line Download PDFInfo
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- CN108522173A CN108522173A CN201611169043.8A CN201611169043A CN108522173A CN 108522173 A CN108522173 A CN 108522173A CN 201611169043 A CN201611169043 A CN 201611169043A CN 108522173 A CN108522173 A CN 108522173A
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- stem rot
- inbred line
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- tolerant corn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of screening techniques of shade tolerant corn inbred line.Corn material to be screened is planted when 3 August, planting density is 67500 plants/hm2；Using 2 factor split block designs, strain is primary area, and illumination is secondary area, and 2 processing of 90% shading rate of Low Light Density Stress and normal illumination are arranged, repeat three times, uniline area, and row is 4 meters long, 0.8 meter of line-spacing, using simple grain nursery, the transplanting of 1 heart stage of 2 leaf, single-strain planting；Start to be shaded into limit by row with the sunshade net of shading rate 90% in 56 leaf phases, 3 months time；In the stage of yellow ripeness, stem rot incidence is investigated；Data statistics and analysis are carried out using DPS statistical softwares；Shade tolerant corn inbred line is filtered out according to diseased plant rate.The method of the present invention is to according to stem rot, that a situation arises is more direct to screen shade tolerant corn material, and it is convenient to record, and error is small, simple and practicable, quickly, accurate.
Description
Technical field
The present invention relates to plant breeding more particularly to a kind of screening techniques of shade tolerant corn inbred line.
Background technology
Southwestern China area is based on hilly and mountainous land, and long-term cloud and mist is more, sunshine is few, humidity is big, performance is prolonged throughout the year
Overcast and rainy low-light synoptic process.Under the conditions of overcast and rainy low-light, intensity of illumination is insufficient, and photosynthesizer is not normally functioning,
Soil, air are chronically at saturated humidity state, and vine growth and development is bad, encounter continuous wet weather, pollination fertilization in florescence
Process is obstructed, and setting percentage reduces, and grouting is bad, and pest and disease damage increases.Southwest planting patterns nonirrigated farmland is special based on interplanting
Weather conditions and cropping system are an important factor for restricting maize production.Carry out resistance to shady corn breeding, is to adapt to southwestern low-light
Special ecological weather conditions and the needs for cultivating suitable spacing sleeve cropping kind, play an important role to improving Southwest Maize yield.It is resistance to
The screening and identification of cloudy corn material are premise and basis that shade tolerant corn variety is cultivated.
By shading treatment, forefathers are under Low Light Density Stress to corn growth and morphogenesis, yield, quality, physiology
The influence of biochemical aspect possesses some special knowledge, and preliminary screening has also been carried out to the shade tolerance index of corn variety, to corn inbred line material
Expect that the research under dark and damp environment is not reported.
Inbred causes its hereditary basis narrow during gradually selfing purifying, viability and hybridization
Kind compares, and has a certain distance.This research is by sunshade net Shading treatment, and research shade environment is to inbred plant
The influence of character, it is intended to the shade tolerance of corn material be evaluated, shade tolerant corn material is screened, provided for shade tolerant corn breeding
Foundation.
Invention content
The purpose of the present invention is to provide a kind of simple, the screening techniques of accurate shade tolerant corn inbred line.
To achieve the above object, the present invention provides a kind of screening technique of shade tolerant corn inbred line, which is characterized in that method
For,
Plantation：Corn material to be screened is planted when the 3-8 months, planting density is 67500 plants/hm2；Using 2 factor split plots
Design, strain is primary area, and illumination is secondary area, and 2 processing of 90% shading rate of Low Light Density Stress and normal illumination are arranged, repeat three times,
Uniline area, row is 4 meters long, 0.8 meter of line-spacing, using simple grain nursery, the transplanting of 1 heart stage of 2 leaf, single-strain planting；Start with screening in the 5-6 leaf phases
The sunshade net of light rate 90% shades into limit by row, 3 months time；
Trial：Base manure is 450 kilograms/hm of phosphate fertilizer2, 75 kilograms/hm of urea2, 75 kilograms/hm of potash fertilizer2, chicken manure water
11250 kilograms/hm2；Jointing fertilizer：180 kilograms/hm of urea2；Attack 180 kilograms/hm of luxuriant fertile urea2；
Data and domain analysis：In the stage of yellow ripeness, stem rot incidence is investigated；Using DPS statistical softwares carry out data statistics with
Analysis；
As a result judge：Shade tolerant corn inbred line is filtered out according to the stem rot diseased plant rate of the data statistics and analysis.
The prior art with corn male and female interval, Net Photosynthetic Rate, specific leaf weight, row grain number, dry matter weight of aerial parts amount with
Yield reduces index of the percentage as evaluation corn shade tolerance, and character formation is influenced by multiple factors, and assay method is more complex,
Continuous mode test error is big.
The discovery of the present inventor's creativeness：Shade is more direct to maize diseases generation, and it is convenient to record, error
It is small, it is simple and practicable；Quickly, simply, accurately for the screening of shade tolerant corn inbred line.
Description of the drawings
Fig. 1 is the picture of Shading treatment in embodiment.
Fig. 2 is the picture of natural lighting in embodiment.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.Embodiment
In particular technique or condition person is not specified, according to technology or condition described in document in the art or according to the description of product
Book carries out.Reagents or instruments used without specified manufacturer, being can be with conventional products that are commercially available.
1, it is carried out in Chongqing 3-8 months；
2, density is identified：67500 plants/hm of density2；
3, field planting：Using 2 factor split block designs, strain is primary area, and illumination is secondary area, and Low Light Density Stress (90% is arranged
Shading rate) (see Fig. 1) and two processing of normal illumination (see Fig. 2), it repeats three times, uniline area, row is 4 meters long, and 0.8 meter of line-spacing is adopted
With simple grain nursery, the transplanting of 1 heart stage of 2 leaf, single-strain planting.Start to be hidden into limit by row with the sunshade net of shading rate 90% in the 5-6 leaf phases
Shade, 3 months time；
4, trial：Base manure：450 kilograms/hm of phosphate fertilizer2, 75 kilograms/hm of urea2, 75 kilograms/hm of potash fertilizer2, chicken manure water
11250 kilograms/hm2；Jointing fertilizer：180 kilograms/hm of urea2；Attack 180 kilograms/hm of luxuriant fertile urea2。
5, investigation method：In the stage of yellow ripeness, stem rot incidence is investigated；It carries out data statistics using DPS statistical softwares and divides
Analysis.
6, the generation of stem rot and the field microclimate factor, intensity of illumination are closely related, and high humility, low-light are then fallen ill
Weight, rapid onset；Low humidity, intense light irradiation, then morbidity is slow, and morbidity is light.Test result shows under natural lighting, participate in the experiment material without
Stem rot occurs.After Shading treatment, different degrees of stem rot has occurred in material of respectively participating in the experiment；Variance analysis shows respectively to participate in the experiment
The stem rot diseased plant rate difference of material is extremely notable, it can thus be seen that influence of the shading to different genotype material is different
, stem rot diseased plant rate is the performance in the shade tolerance for material of participating in the experiment.
7, therefore, shade tolerant material can be screened using the stem rot incidence of corn material plant under limit shading condition
Material.
Embodiment 1：The screening of shade tolerant corn inbred line
Test material:The Chongqings 561 of Chongqing Agricultural Academy of Science's selection and breeding, Chongqing 8954, Chongqing 676, Chongqing 1193-9 and introduction from
Friendship be PH4CV, 18-599,4011, tuck in 478.
Experimental design:Test arrangement is in Chongqing Agricultural Academy of Science emperor field proving ground, and topography is flat, in soil fertility
Deng.
Using 2 factor split block designs, strain is primary area, and illumination is secondary area, and setting Low Light Density Stress (90% shading rate) is (see figure
1) it is handled with normal illumination (see Fig. 2) 2,3 repetitions, uniline area, using simple grain culturing and transplanting seedlings, single-strain planting mode, density
67500 plants/hm2.Start to be shaded with the sunshade net of shading rate 90% in the 5-6 leaf phases, 3 months time.
Measurement project and method:In the stage of yellow ripeness, stem rot incidence is investigated, the results are shown in Table 1；Using DPS statistical softwares into
Line number with analysis, the results are shown in Table 2 according to statistics.
Table 1 is participated in the experiment material stem rot incidence list position:%
Table 2 is participated in the experiment material stem rot diseased plant rate variance analysis Multiple range test table
Shade tolerance comparison in difference under Shading stress：Show under natural lighting from the test result of table 1 and 2, material of participating in the experiment
Material occurs without stem rot；After Shading treatment, different degrees of stem rot has occurred in material of respectively participating in the experiment；Variance analysis shows respectively
Participate in the experiment material stem rot incidence difference it is extremely notable, the stem rot incidence sequence for material of participating in the experiment is：Chongqing 8954 is tucked in
478, Chongqing 676,18-599,4011, Chongqing 1193-9, Chongqing 561, PH4CV.
The generation of stem rot and the field microclimate factor, intensity of illumination are closely related, high humility, low-light, then weight of falling ill,
Rapid onset；Low humidity, intense light irradiation, then morbidity is slow, and morbidity is light.It can thus be seen that influence of the shading to different genotype material
Be different, stem rot incidence be participate in the experiment material shade tolerance performance, material shade tolerant degree of participating in the experiment is from high to low
PH4CV, Chongqing 561, Chongqing 1193-9,4011,18-599, Chongqing 676, tuck in 478, Chongqing 8954.
According to corn disease and insect resistance identification technology specification, the 7th part, the anti-fusarium stem rot identification technology specification of corn, hair
Diseased plant rate 0%~1.0% is highly resistance, and diseased plant rate 1.1%~5.0% is anti-, and diseased plant rate 5.1%~10.0% is moderate resistance,
Diseased plant rate 10.1%~40.0% is sense, and diseased plant rate 40.1%~100% is high sense；According to the stem rot of 1 each strain of table
Incidence may determine that：PH4CV, Chongqing 561 are shade tolerant corn inbred line.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case of can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (1)
1. a kind of screening technique of shade tolerant corn inbred line, which is characterized in that method is,
Plantation：Corn material to be screened is planted when the 3-8 months, planting density is 67500 plants/hm2；Using 2 factor split block designs,
Strain is primary area, and illumination is secondary area, and 2 processing of 90% shading rate of Low Light Density Stress and normal illumination are arranged, repeat three times, uniline
Area, row is 4 meters long, 0.8 meter of line-spacing, using simple grain nursery, the transplanting of 1 heart stage of 2 leaf, single-strain planting；Start to use shading rate in the 5-6 leaf phases
90% sunshade net shades into limit by row, 3 months time；
Trial：Base manure is 450 kilograms/hm of phosphate fertilizer2, 75 kilograms/hm of urea2, 75 kilograms/hm of potash fertilizer2, chicken manure water 11250 it is public
Jin/hm2；Jointing fertilizer：180 kilograms/hm of urea2；Attack 180 kilograms/hm of luxuriant fertile urea2；
Data and domain analysis：In the stage of yellow ripeness, stem rot incidence is investigated；Data statistics and analysis are carried out using DPS statistical softwares；
As a result judge：Shade tolerant corn inbred line is filtered out according to the stem rot diseased plant rate of the data statistics and analysis.
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| CN201611169043.8A CN108522173A (en) | 2016-12-16 | 2016-12-16 | A kind of screening technique of shade tolerant corn inbred line |
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| CN201611169043.8A CN108522173A (en) | 2016-12-16 | 2016-12-16 | A kind of screening technique of shade tolerant corn inbred line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699425A (en) * | 2019-03-18 | 2019-05-03 | 漯河市农业科学院 | A kind of method of breeding green high-efficient peanut varieties |
| CN111328653A (en) * | 2020-03-13 | 2020-06-26 | 沧州市农林科学院 | Corn anti-black cutworm evaluation field bioassay method |
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| CN103392405A (en) * | 2013-07-04 | 2013-11-20 | 甘肃农业大学 | Simple screening method for salt-tolerance maize inbred lines |
| CN103975843A (en) * | 2014-05-08 | 2014-08-13 | 郯城县种子公司 | Introduction aggregation breeding method of maize inbred line |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102487809A (en) * | 2011-11-16 | 2012-06-13 | 中国科学院亚热带农业生态研究所 | Method of breeding high-yield multi-resistance corn single hybrid |
| CN103392405A (en) * | 2013-07-04 | 2013-11-20 | 甘肃农业大学 | Simple screening method for salt-tolerance maize inbred lines |
| CN103975843A (en) * | 2014-05-08 | 2014-08-13 | 郯城县种子公司 | Introduction aggregation breeding method of maize inbred line |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699425A (en) * | 2019-03-18 | 2019-05-03 | 漯河市农业科学院 | A kind of method of breeding green high-efficient peanut varieties |
| CN109699425B (en) * | 2019-03-18 | 2021-01-08 | 漯河市农业科学院 | Method for breeding green and efficient peanut varieties |
| CN111328653A (en) * | 2020-03-13 | 2020-06-26 | 沧州市农林科学院 | Corn anti-black cutworm evaluation field bioassay method |
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