CN108472318A - 人源化心脏肌肉 - Google Patents

人源化心脏肌肉 Download PDF

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Publication number
CN108472318A
CN108472318A CN201680050242.9A CN201680050242A CN108472318A CN 108472318 A CN108472318 A CN 108472318A CN 201680050242 A CN201680050242 A CN 201680050242A CN 108472318 A CN108472318 A CN 108472318A
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CN
China
Prior art keywords
nkx2
tbx5
handii
combinations
cell
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Pending
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CN201680050242.9A
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English (en)
Chinese (zh)
Inventor
D·J·加里
M·G·加里
N·小谷野-中川
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University of Minnesota System
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University of Minnesota System
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Publication of CN108472318A publication Critical patent/CN108472318A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
CN201680050242.9A 2015-06-30 2016-06-30 人源化心脏肌肉 Pending CN108472318A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562187040P 2015-06-30 2015-06-30
US62/187,040 2015-06-30
PCT/US2016/040431 WO2017004388A1 (en) 2015-06-30 2016-06-30 Humanized heart muscle

Publications (1)

Publication Number Publication Date
CN108472318A true CN108472318A (zh) 2018-08-31

Family

ID=57609529

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680050242.9A Pending CN108472318A (zh) 2015-06-30 2016-06-30 人源化心脏肌肉

Country Status (13)

Country Link
US (2) US10897880B2 (enExample)
EP (1) EP3316899A4 (enExample)
JP (1) JP2018523999A (enExample)
KR (1) KR20180021135A (enExample)
CN (1) CN108472318A (enExample)
AU (1) AU2016288671A1 (enExample)
BR (1) BR112017028608A2 (enExample)
CA (1) CA2991056A1 (enExample)
CO (1) CO2018000859A2 (enExample)
IL (1) IL256544A (enExample)
MX (1) MX2018000071A (enExample)
RU (1) RU2018103234A (enExample)
WO (1) WO2017004388A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10874092B2 (en) 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
US10897880B2 (en) 2015-06-30 2021-01-26 Regents Of The University Of Minnesota Humanized heart muscle
US11673928B2 (en) 2015-03-03 2023-06-13 Regents Of The University Of Minnesota Genetically modified pig cells with an inactivated Etv2 gene

Citations (4)

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CN1212009A (zh) * 1996-01-09 1999-03-24 加利福尼亚大学董事会 有蹄动物胚胎干样细胞、制法及用该细胞产生转基因的有蹄动物的方法
CN1241210A (zh) * 1996-10-11 2000-01-12 得克萨斯农业及机械体系综合大学 产生原生殖细胞和转基因动物物种的方法
CN102438636A (zh) * 2009-05-20 2012-05-02 卡迪欧参生物科技有限公司 用于治疗心脏疾病的药物组合物
US20150140658A1 (en) * 2013-11-15 2015-05-21 Wisconsin Alumni Research Foundation LINEAGE REPROGRAMMING TO INDUCED CARDIAC PROGENITOR CELLS (iCPC) BY DEFINED FACTORS

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US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
WO2003034818A1 (en) 2001-10-24 2003-05-01 Chugai Seiyaku Kabushiki Kaisha Sgrf genetically modified nonhuman animal
US20040133931A1 (en) 2003-01-08 2004-07-08 Gavin William G. Method and system for fusion and activation following nuclear transfer in reconstructed embryos
WO2003081990A2 (en) 2002-03-22 2003-10-09 St. Jude Children's Research Hospital Improved method for generating genetically modified animals
WO2004004447A2 (en) * 2002-07-03 2004-01-15 Merck & Co., Inc. Agouti-related protein deficient cells, non-human transgenic animals and methods of selecting compounds which regulate energy metabolism
GB2398784B (en) * 2003-02-26 2005-07-27 Babraham Inst Removal and modification of the immunoglobulin constant region gene cluster of a non-human mammal
WO2006014551A2 (en) 2004-07-06 2006-02-09 Michigan State University In vivo methods for effecting tissue specific differentiation of embryonic stem cells
WO2008086040A1 (en) 2007-01-11 2008-07-17 University Of Pittsburgh Muscle derived cells for the treatment of urinary tract pathologies and methods of making and using the same
CA2675100A1 (en) 2007-01-20 2008-07-24 Boehringer Ingelheim International Gmbh C-reactive protein (crp) knockout mouse
JPWO2008102602A1 (ja) 2007-02-22 2010-05-27 国立大学法人 東京大学 Ｂｌａｓｔｏｃｙｓｔｃｏｍｐｌｅｍｅｎｔａｔｉｏｎを利用した臓器再生法
JP5688788B2 (ja) 2008-02-22 2015-03-25 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
GB2475656B (en) 2008-08-22 2013-04-24 Univ Tokyo Organ regeneration method utilizing ips cell and blastocyst complementation
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JP6158170B2 (ja) 2011-04-27 2017-07-12 アミリス， インコーポレイテッド ゲノム修飾のための方法
EP3354343B1 (en) * 2011-06-02 2023-09-06 President and Fellows of Harvard College Methods and uses for ex vivo tissue culture systems
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IL221187A (en) 2012-07-30 2017-01-31 Adom Advanced Optical Tech Ltd A system to perform optical tomography in two 2D beams
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
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WO2016141234A1 (en) 2015-03-03 2016-09-09 Regents Of The University Of Minnesota Etv2 and uses thereof
CN108135943A (zh) 2015-06-30 2018-06-08 明尼苏达大学校董会 人源化的骨骼肌
BR112017028608A2 (pt) 2015-06-30 2018-09-04 Univ Minnesota músculo cardíaco humanizado
KR20180100303A (ko) 2015-10-27 2018-09-10 리젠츠 오브 더 유니버시티 오브 미네소타 키메라 배아-보조 기관 생성을 위한 조성물 및 방법
CN108125943A (zh) 2018-01-17 2018-06-08 云南省第二人民医院 前胡醇当归脂作为制备抗动脉粥样硬化药物的应用

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CN1212009A (zh) * 1996-01-09 1999-03-24 加利福尼亚大学董事会 有蹄动物胚胎干样细胞、制法及用该细胞产生转基因的有蹄动物的方法
CN1241210A (zh) * 1996-10-11 2000-01-12 得克萨斯农业及机械体系综合大学 产生原生殖细胞和转基因动物物种的方法
CN102438636A (zh) * 2009-05-20 2012-05-02 卡迪欧参生物科技有限公司 用于治疗心脏疾病的药物组合物
US20150140658A1 (en) * 2013-11-15 2015-05-21 Wisconsin Alumni Research Foundation LINEAGE REPROGRAMMING TO INDUCED CARDIAC PROGENITOR CELLS (iCPC) BY DEFINED FACTORS

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11673928B2 (en) 2015-03-03 2023-06-13 Regents Of The University Of Minnesota Genetically modified pig cells with an inactivated Etv2 gene
US10874092B2 (en) 2015-06-30 2020-12-29 Regents Of The University Of Minnesota Humanized skeletal muscle
US10897880B2 (en) 2015-06-30 2021-01-26 Regents Of The University Of Minnesota Humanized heart muscle
US12089574B2 (en) 2015-06-30 2024-09-17 Regents Of The University Of Minnesota Humanized skeletal muscle

Also Published As

Publication number Publication date
EP3316899A1 (en) 2018-05-09
AU2016288671A1 (en) 2018-02-01
CO2018000859A2 (es) 2018-07-10
US20180177165A1 (en) 2018-06-28
KR20180021135A (ko) 2018-02-28
US10897880B2 (en) 2021-01-26
CA2991056A1 (en) 2017-01-05
MX2018000071A (es) 2018-03-16
BR112017028608A2 (pt) 2018-09-04
WO2017004388A1 (en) 2017-01-05
JP2018523999A (ja) 2018-08-30
EP3316899A4 (en) 2019-04-03
US20210169054A1 (en) 2021-06-10
RU2018103234A (ru) 2019-07-31
IL256544A (en) 2018-02-28

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Application publication date: 20180831

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