CN108450656A - A kind of method that Chinese herb residues orientation fermentation prepares functional feedstuff additive - Google Patents

Abstract

The invention discloses a kind of methods that Chinese herb residues orientation fermentation prepares functional feedstuff additive.The method that the Chinese herb residues orientation fermentation prepares functional feedstuff additive is the various disease feature for animal, Chinese herb residues is classified according to the pharmacological function of its crude drug, in（Beast）Medical knowledge opinion carries out selection and prescription, then adds basal nutrient material and prepare druggability matrix, is inoculated with and the same or analogous medicine of dregs of a decoction property function into fermentation substrate after sterilizing（Food）With hypha,hyphae strain, the medicinal fungal substance for surmounting prescription Chinese medicine slag function is obtained after fermentation, up to the feed addictive of specific function after thalline self-dissolving or not self-dissolving, drying, crushing；In the pharmacological property matrix, the mass percent of basal nutrient material is 10-30%；The inoculum concentration of the medicinal fungi hypha bacterial is 5-20%.

Description

A kind of method that Chinese herb residues orientation fermentation prepares functional feedstuff additive

Technical field

The present invention relates to a kind of methods that Chinese herb residues orientation fermentation prepares functional feedstuff additive.

Background technology

Chinese herb residues are an extensive and abundant resources, it was reported that about 30,000,000 tons of national annual emissions, with traditional Chinese medicine And its development of public health work, yield are also being continuously increased；How to be scientifically that current low-carbon of advocating is given birth to using this resource One of important research content in living, energy conservation and environmental protection and green circulation economy theme.Numerous studies show that Chinese medicine slag is removed containing big Outside the nutriments such as protein, fat, the trace element of amount, contain the pharmacological property ingredient not extracted much；Chinese medicine slag at present Using mainly other active ingredients is extracted again, it is used for culturing edible fungus, is used as feed or feed addictive, biomass energy, Paper making raw material, fermenting and producing organic fertilizer and processing waste etc..

Chinese medicine slag is crushed has nutrition adjustment, improvement meat product quality as feed addictive, enhances immune, adjusting swashs The effects that element, anti-bacteria and anti-virus, health-related Chinese medicine slag raising animal can health care growth promotion, improve production performance；Coptis root, root of Aucklandia lappa Decne, The application of the dregs of a decoction such as evodia rutaecarpa, Schisandra chinensis can prevent livestock and poultry fish disease of digestive tract, fin rot, play the work of antibiotic and insecticide With.

Chinese medicine slag biological fermentation feed is studied with functional feedstuff additive, currently, Chinese medicine slag biological fermentation feed and feeding Feed additives are studied, and more limitations produce probiotic feed additive with probiotics, saccharomycetes to make fermentation, with filamentous fungi (whiterot fungi, Aspergillus etc.) fermentation production of protein feedstuff or with food medicine fungi fermentation produce additive of protein feed.Its fermented feed or feed add It is degradation crude fibre to add agent mainly consideration index, improves protein content, increases probiotics etc., is disappeared with balanced nutrients, raising Consumption rate, enhancing is immunized and premunition, improves weightening and breeding potential.

The main raising and improvement for considering product nutrient ingredient of screening of Chinese herb residues fermenting microbe, enhancing digestive enzyme, Antimicrbial power is improved, such as burnt skilful fragrant (2010) filter out trichoderma asperellum fermented tcm slag from numerous bacterium, make its nutritional ingredient significantly Improve；Studied in terms of beast herb fermenting more, bacterial screening is mainly used for fermentation of Chinese herbal medicine substitute antibiotics, when such as blue Happy (2010) report aspergillus oryzae fermentation of Chinese herbal medicine, Guo Shu (2015) fermentation of bacillus subtilis compound Chinese herb, Zhang Libin (2012) composite probiotics ferment Chinese herbal composite feed additive.

With fungi other than containing abundant nutriment, also various medicinal functions are widely used in medicine, protect medicine (food) Health food industry, is also widely used in the fermentation of Chinese herbal medicine and the dregs of a decoction, but is mostly " bi-directional fermentation ", i.e., Chinese herbal medicine prepares culture medium (pharmacological property matrix) medication food fungi bacteria fermentation (production medicinal fungal substance), Chinese herbal medicine provides nutrition for medicine food fungi bacteria, and medicine food is true in turn It is active ingredient that mycelium, which secretes some invalid components in various enzymatic conversion Chinese herbal medicines, during bacterium growth and breeding, improves drug Function.But this method fails to consider that the homoplasy of the two, tool bear the character of much blindness, take a lot of work on Chinese herbal medicine and strain combination Time-consuming, success rate is low.

The dregs of a decoction contain a large amount of available nutritional ingredients and pharmacological property ingredient, have broad application prospects, but simultaneously because medicine The diversity of material, the complexity of ingredient, there are many technical problems when the dregs of a decoction being caused to recycle, such as：

1, the combination of fermenting microbe selection, Chinese medicine slag and strain lacks scientific, bears the character of much blindness and randomness.

2, the pharmacological property using remaining effective component and crude drug well is failed to the utilization of Chinese medicine slag, mostly by it It is utilized as common coarse fodder or industry byproduct.

3, the pharmacological property of medical edible fungal is failed scientifically with the fermentation applied to Chinese herb residues, fermented product is not It can fully consider the raising or improvement of property function.

4, Chinese herb residues fermented product pursues the improvement and raising of feed nutritive value mostly, such as the increasing of protein content Add, the decline etc. of content of cellulose, lacks the further investigation to its pharmacological function and its ingredient.

5, products application is confined to be used as conventional feed or general raising immunity functional feed additive mostly, fails It is reasonably used for the application of animal diseases control.

In addition, the fermentation research of current Chinese medicine slag, is carried out primary using the dregs of a decoction as the culture base-material of general (or special) Property fermentation, have single culture fermentation, also there is composite bacteria fermentation；There is single medicinal material (slag) fermentation, also there is compound (side) Chinese medicine (slag) ferments.Whether any fermentation is all based on the improvement of nutrient characteristics and (improves the content of crude protein, polysaccharide etc., reduce thick Fiber content etc.), the raising with general utility functions (improving immunity, resisting stress, antibacterial etc.) is used as general nutrition and health care and raises Feed additives or feed.It is few as fermenting microbe research using medicine food fungi bacteria, the Changeement of the substances such as polysaccharide is mainly carried out, Among pharmacological properties without holding medicine (food) fungi well are applied to the fermentation of Chinese medicine and Chinese medicine slag, make medicinal fungi in Homogeneous promotion is accomplished in medicine or Chinese medicine slag fermentation, realizes the addition or multiplication of mycoplasma pharmacological effect function.Chinese medicine slag application at present The research of animal is to be based on its nutritional resource mostly, although knowing residual effective component and Related Component therein, is neglected mostly Scientific development application has been omited, has directly crushed or simple biofermentation is used as feed or feed addictive, do not regarded as medicine Material or prescription medicine (low-quality) go to excavate and be promoted, and carry out secondary development research under tcm theory guidance, give full play to Chinese medicine The pharmacological effect function of slag is used for the prevention of Animal diseases.About zymotechnique, generally in two steps (culture test of goodness of fit with Product measures) or three steps (Zhuan Yi propose " three layers of optimum seeking method ") selection method determine the fementative composition of Chinese medicine (slag) and strain, Resettle corresponding production technology.The direction target of beginning is indefinite, lacks scientific theory guidance, randomness is very big, often wants Walk many detours.The aftertreatment technology of tunning is simple-and it is mostly drying and crushing, thalline fails to crack, and active ingredient digestion is inhaled Yield is low.

Invention content

Functional feed is prepared the present invention is directed to overcoming the deficiencies of the prior art and provide a kind of Chinese herb residues orientation fermentation to add Add the method for agent.

In order to achieve the above object, technical solution provided by the invention is：

The method that the Chinese herb residues orientation fermentation prepares functional feedstuff additive is by Chinese herb residues by its former prescription Function and usage classification, according to animal different syndromes with Chinese veterinarian's theory be guidance carry out selection and prescription, then with basal nutrient Material prepares druggability matrix, and inoculation is used with the same or analogous medicine of prescription dregs of a decoction pharmacological function (food) in chemotropism matrix after sterilizing Hypha,hyphae strain obtains the medicinal fungal substance with specific function after fermentation, after thalline self-dissolving or not self-dissolving, drying, crushing i.e. Obtain functional feedstuff additive；In the pharmacological property matrix, the mass percent of basal nutrient material is 10-30%；It is described medicinal true The inoculum concentration of bacterium hypha bacterial is 5-20%.

Preferably, the basal nutrient material includes the agricultural and sideline products such as corn flour, bean cake powder, flour and mineral element growth promotion Factor etc..

Preferably, the functional feedstuff additive includes anti-cures cold and the feed addictive of influenza, prevention breathing system The feed addictive for disease of uniting, the feed addictive for preventing genital system diseases, is prevented the feed addictive for preventing disease of digestive tract The feed addictive of systemic disease, the feed addictive of tranquilizing the mind and Metabolism regulation, prevention rheumatism and the feed of Wai Bing sections is controlled to add Agent, the increasing of extra-nutrition antibacterial is added to exempt from somatotrophic feed addictive.

Preferably, the flu and influenza include anemofrigid cold, anemopyretic cold, virus flu；The respiratory system disease Disease includes acute inflammation, chronic inflammation；The disease of digestive tract includes acute gastroenteritis, chronic enterogastritis, constipation, liver and gall synthesis Sign；The genital system diseases include Reproduction Disorder, postpartum disease, mammitis；The systemic disease includes qi and blood Weak disease, febrile illness；The tranquilizing the mind and Metabolism regulation include resisting stress, adjust metabolism；The rheumatism includes with Wai Bing sections diseases Rheumatic Bi syndrome, surgery inflammation etc..

Preferably, when preparing the feed addictive of prevention anemofrigid cold, Chinese herb residues are selected from the pungent-warm for the treatment of anemofrigid cold The inducing diaphoresis class Chinese patent drug dregs of a decoction, fungi are selected from medicine/edible fungus of warm-natured sweating cold-dispelling；The feed for preparing prevention anemopyretic cold adds When adding agent, the refrigerant inducing diaphoresis Chinese patent drug dregs of a decoction of the Chinese herb residues selected from treatment anemopyretic cold, medicine of the fungi selected from dispelling wind and heat from the body inducing diaphoresis/ Edible fungus；When preparing the feed addictive of prevention virus flu, Chinese herb residues are selected from clearing heat and detoxicating Chinese patent drug of ventilatining the lung and resolving phlegm The dregs of a decoction, fungi are selected from clearing heat and detoxicating, phlegm-eliminiating and qi-regulating medicine/edible fungus；Prepare the feed addition of prevention acute respiratory inflammation When agent, Chinese herb residues are selected from the dregs of a decoction of clearing heat, eliminating phlegm, relieving cough Chinese patent drug, and fungi is selected from the medicine/edible fungus for controlling cough with lung heat；System When the feed addictive of standby prevention chronic airway inflammation, Chinese herb residues are selected from moistening lung, resolving sputum, cough-relieving, the Chinese patent drug relievingd asthma The dregs of a decoction, fungi are selected from tonifying Qi and blood, relieving cough and asthma medicine/edible fungus；When preparing the feed addictive of prevention acute gastroenteritis, Chinese herb residues are selected from the Chinese patent drug slag of heat-clearing and damp-drying drug treatment enterogastritis, medicine/edible fungus that fungi is selected from clearing heat and promoting diuresis, controls dysentery； When preparing the feed addictive of prevention chronic enterogastritis, Chinese herb residues are selected from warm stomach invigorating the spleen, tonifying middle-Jiao and Qi, in promoting digestion and removing indigestion at The dregs of a decoction, medicine/edible fungus that fungi is selected from invigorating the spleen to arrest diarrhea and stomach helps digestion；When preparing the anti-feed addictive to treat constipation, Chinese herbal medicine Slag is selected from clearing away heat and moistening the bowels, pleasant to lead stagnant Chinese patent drug slag, and fungi is selected from the medicine/edible fungus for filling blood, relaxing bowel；It prepares anti- When controlling the feed addictive of liver and gall syndrome, Chinese herb residues are selected from dispersing stagnated hepatoqi, the clearing heat and expelling damp Chinese patent drug dregs of a decoction, and fungi is selected from clear Thermal detoxification, anti-inflammatory, invigorating spleen to remove dampness, liver protecting medicine/edible fungus；Prepare the feed addictive of prevention Reproduction Disorder When, Chinese herb residues are selected from tonifying kidney and strengthening yang, the Chinese patent drug or the health food dregs of a decoction of invigorate blood circulation, and fungi is selected from tonifying kidney and strengthening yang, nourishing Medicine/edible fungus of body-building；Prepare prevention postpartum disease feed addictive when, Chinese herb residues be selected from heat-clearing, promoting blood circulation, dissolving stasis, The gynaecology of analgesic and the cardiovascular disease Chinese patent drug dregs of a decoction, fungi are selected from promoting blood circulation and hemostasis, anti-inflammatory dissolving stasis, the medicine under uterine bleeding band/edible Fungi；When preparing the feed addictive of prevention mammitis, Chinese herb residues are selected from clearing heat and detoxicating, blood stasis dispersing and deswelling, promoting blood circulation and stopping pain, soothing the liver The Chinese patent drug slag of mammitis Chinese medicine slag and dredging collateral for promoting lactation is treated in qi-regulating, fungi be selected from have it is clearing heat and detoxicating, the medicine of detumescence and apocenosis function/ Edible fungus；When preparing the feed addictive of prevention deficient qi and blood disease, Chinese herb residues are selected from bowl spares invigorating the spleen, enriching the blood and tonifying qi Chinese patent drug Or the dregs of a decoction of health food, fungi are selected from tonifying spleen appetizing, drugs for tonifying Qi and blood/edible fungus；Prepare the feed of prevention febrile illness When additive, Chinese herb residues are selected from the Chinese patent drug dregs of a decoction of clearing away heat,cooling blood and removing toxin, and fungi is selected from anti-bacteria and anti-virus, cooling blood and hemostasis Medicine/edible fungus；When preparing the feed addictive of resisting stress, Chinese herb residues are selected from blood pressure lowering, and the tranquilizing the mind for the treatment of coronary heart disease etc. is peaceful At the prescription dregs of a decoction, fungi is selected from tool tranquilizing and allaying excitement, nourishes brain tonic, medicine/edible fungus that calming heart is healthy and strong the heart；It prepares and adjusts metabolism When feed addictive, Chinese herb residues are selected from the Chinese patent drug dregs of a decoction of the metabolic diseases such as hyperlipidemia, diabetes, high cholesterol treatment, Fungi is selected from medicine/edible mushroom of hypoglycemic, blood fat, cholesterol；When preparing the feed addictive of prevention rheumatic Bi syndrome, Chinese herb residues Selected from clearing and activating the channels and collaterals, wind-dispelling clearing damp treatment rheumatism bone disease Chinese patent drug slag and the promoting flow of qi and blood circulation, stagnation resolvation are promoted blood circulation and are treated in cardiovascular disease At the dregs of a decoction, fungi is selected from promoting blood circulation and removing obstruction in channels, wind-expelling and dispelling dampness, the medicine/edible fungus to strengthen muscles and bones；Prepare the feed of prevention surgical disease When additive, Chinese herb residues be selected from skin and mucosa inflammation disease treat Chinese patent drug slag, fungi be selected from it is clearing heat and detoxicating, treatment carbuncle, subcutaneous ulcer, Medicine/edible fungus of sore, furuncle, tinea.The somatotrophic feed addictive of extra-nutrition antibacterial is prepared, it is low to can be selected from single fiber content Residue or other prescription (such as tumour medication, ophthalmic remedy, ear nose larynx and mouths after Chinese herbal medicine full of nutrition and animals and plants extraction Chamber section medication etc.) Chinese patent drug the dregs of a decoction and the preparations such as pill, powder, tablet some Chinese Patent Medicines extraction after the dregs of a decoction；It is described true Bacterium is selected from that grow fast protein content high and have medicine/edible fungus of antibacterial functions or with same or similar with dregs of a decoction active compound function Medicine/edible fungus.

Preferably, Chinese medicine slag prescription includes classical or curative for effect in the individual singles lacked in square Chinese patent drug slag Herbal medicine slag can be used corresponding single Chinese herb residues or Chinese patent drug containing single ingredient to supplement, also include same or similar pharmacological function it is several in The dregs of a decoction combine, and further include adding taste prescription, when such as preparing the feed addictive of the anxious inflammation respiratory tract disease of prevention, in clearing heat, eliminating phlegm, relieving cough The dregs of a decoction or crude drug of heat-clearing poison Chinese patent drug can be added in the dregs of a decoction of patent medicine；When preparation prevents the feed addictive of chronic enterogastritis, Warm stomach invigorating the spleen, tonifying middle-Jiao and Qi, can add in the Chinese patent drug slag for the analgesic that helps digestion the granatum of relieving diarrhea with astringents, myrobalan, dark plum, nutmeg, The dregs of a decoction or crude drug of one or more of red halloysite etc..

Preferably, the relieving the exterior syndrome with drugs pungent in flavor and warm in property class Chinese patent drug dregs of a decoction of the treatment anemofrigid cold include Jiuweiqianghuo, the small Green Dragon Mixture, Fenghanganmao Granules, cough due to wind-cold evil's particle, GANMAO QINGRE KELI, the anti-granules for treating common cold of chaste tree, Kechuanning Granules, cassia twig mixture, Cinnamaldehyde, table reality cold granules, " Zhengcaihu Drink ", almond perilla anti-cough electuary, Chuan Xiong Tea electuary, precious peaceful of cough The dregs of a decoction of grain, pungent a kind of reed mentioned in ancient books particle etc., the medicine (food) of the warm-natured sweating cold-dispelling include wheat loose smut, bitter white hoof, ox with fungi Liver bacterium etc.；The refrigerant inducing diaphoresis Chinese patent drug dregs of a decoction of the treatment fever flu include anemopyretic cold electuary, compound Japanese polygala particle, answer Square lantana piece, flu relaxation grain, GANMAO TUIRE KELI, KANGKAN KELI, anti-sense Jiedu oral liquid, liyan jiedu particle, double Huangs Even particle, wushicha granules, Yinqiaojiedu Capsules, oral liquid of honeysuckle flower and scutellaria, Compound Jinyinhua Granules, Zhisoudingchuankoufuye, Sang Ju Yin It is common cold tablet, Xiao Chai Hu granules, bavin yellow capsule, tablet of antelope's horn for common cold, compound lantana piece, curing cold capsule, Xiaoerjiebiao granules, small The medicine of youngster's Qingrezhike oral solution rhinitis tablet, BIYUANSHU KOUFUYE, bidouyan mixture, bidouyan koufuye, summer-heat cold electuary, Qingliang etc. Slag, the medicine (food) of the dispelling wind and heat from the body inducing diaphoresis include cicada fungus, fragrant apricot dried mushroom, Antrodia camphorata etc. with fungi；The clearing heat and detoxicating facilitaing lung The phlegm-reducing Chinese patent drug dregs of a decoction include " Zhengcaihu Drink ", fragrant snow antiviral oral liquor, tablet of antelope's horn for common cold, banlangen granules, banlangen keli, chaste tree is anti-loses Malicious electuary, compound infusion of isatis root, Xiaoer Feire Kechuan Mixture, heat-clearing and detoxicating oral liquid, even spends clear pest at Shuanghuanglian oral liquid The dregs of a decoction of capsule, KANGKAN KELI, compound Artemisia rupestris particle, cold in children tea, complex (yin huang) liquid etc., it is described it is clearing heat and detoxicating, The medicine (food) of phlegm-eliminiating and qi-regulating includes wild rice bacterium, Antrodia camphorata, handle mushroom etc. with fungi；The dregs of a decoction of the clearing heat, eliminating phlegm, relieving cough Chinese patent drug Including compound Puqi capsules, FUFANG YUXINGCAO PIAN, compound Folium Isatidis mixture, Zhike Juhong Oral Liquid, loquat cough-relieving electuary, numb apricot Cough syrup, GUILONG KECHUANNING JIAONANG, fritillary bulb cough syrup, Yinhuang lung clearing capsule, QINGRE XIAOYANNING PIAN, Tai Ji jizhi syrup, Reyanning particle, children's stemona root cough relieving syrup, cough with lung heat of children oral solution, Qinghuozhimaipian, qianbai biyan tablets, Kowloon removing toxic substances The dregs of a decoction of the dregs of a decoction of capsule etc., the heat-clearing poison Chinese patent drug include oral liquid of honeysuckle flower and scutellaria, CHUANXINLIAN JIAONANG, houttuynia cordata injection, Pu The dregs of a decoction of Herba Taraxaci Tabellae electuary, Herba Hedyotidis Diffusae injection, the distilled liquid of honeysuckle, YIQING KELI, the medicine (food) for controlling cough with lung heat are used Fungi includes golden ear, white fungus, pisolithus tinctorius Coker et Coucs, tabasheer etc.；The dregs of a decoction of the Chinese patent drug of the moistening lung, resolving sputum, cough-relieving include bulbus fritilariae snow pear Cream, gram cough fast active capsule, Xuanmai Ganju grains, erdong gao, bronchitis piece, white apricot piece, consolidates cough and asthma at Extractum Fel Serpentis et Bulbus Fritillariae Cirrhosae et Radix Saurauiae Tristylae The dregs of a decoction of piece, Juhong Tanke liquid, golden fruit lozenge, kouyanqing granules etc., the tonifying Qi and blood, relieving cough and asthma medicine (food) use fungi Including ganoderma lucidum, hidden pore fungi, Cordyceps militaris, bitter white hoof, Uricularia polytricha, dictyophora phalloidea, black fungus, Pleurotus tuber-regium etc.；The heat-clearing and damp-drying drug treats intestines The Chinese patent drug cinder ladle of gastritis includes 'Fengliaochangweikang' ' capsule, HuoXiangZhengQiShui, a kind of reed mentioned in ancient books in flakes, it is Gegen Qinlian Tablets, summer-heat damp cold particle, clear Heat dehumidifying anti-diarrhea granule, removing toxic substances anti-diarrheal capsule, nine taste antipyretic capsules, the special quick particle of dysentery, anti-diarrhea granule, fly upward enterogastritis capsule, Intestines health piece, anti-inflammation medicine piece, antiphlogistic Chunwang capsule, Gentianopsis barbata particle, compound quassia dispersion tablet of diminishing inflammation, YANKENING PIAN, Zhuo Dan Zhixieling of antidiarrheal, The dregs of a decoction of gold and silver dew, compound agrimony enteritis capsule, heat disease piece, rotor angle predicting, HUDI JIAONANG etc., the heat-clearing and damp-drying drug treatment The dregs of a decoction that the Chinese patent drug cinder ladle of enterogastritis includes infirmity clear piece, drenches particle, PAISHI KELI clearly, the clearing heat and promoting diuresis, the medicine (food) for controlling dysentery Include foliosous parnassia herb, trametes robinioplila Pleurotus nebrodensis, gloeostereum incarnatum, delicious lactarius, gold mushroom etc. with fungi；The temperature stomach invigorating the spleen, tonifying middle-Jiao and Qi help digestion only The Chinese patent drug cinder ladle of pain includes small middle mixture, ginseng art invigorating the spleen electuary, the Zhuo Dan compounds built and helps digestion electuary, oral mushroom liquor, children's abdomen Rush down peaceful syrup, stomach soothing medicinal granules, WeiLing Granules, infusion for Soothing The Stomacs, sand stomach-nourishing electruary, SANJIU WEITAI JIAONANG, army's bell instant particle for gastrosis, stomach Peaceful piece, middle calming tablet, gastralgia tablet, ERKANGNING TANGJIANG, change product oral solution, digestion-promoting stomachic, Wei Shuning granule, cold of insufficiency type and stomachache particle, The medicine of yin deficiency and gastralgia particle, qizhi weitong granules, Fuweisan Tablets, gastric blood flow, Jianwei Yuyang Granule, KUIYANGLING JIAONANG etc. The medicine (food) that slag, the invigorating the spleen to arrest diarrhea and stomach help digestion with fungi include Agrocybe chaxingu, hickory chick, agrocybe, coprinus comatus, Hericium erinaceus, Mushroom, hexagonia apiaria, cup club fungi, bolete, Poria cocos, white fungus etc.；The clearing away heat and moistening the bowels, it is pleasant to lead stagnant Chinese patent drug cinder ladle and include Sanhuang tablets, sanhuang pian, detoxicating tablet of cow-bezoar, Fanxieye soluable preparation, Jiangzhi Jianfei Tablets, rheum officinale Tongbian Chongji Zhu Yan Yang Lu Ma Jian, shou wu pian, desert cistanche oral liquid as cathartics, ox The dregs of a decoction of clearing capsule, " Jingwu " medical powder taken with water, tablets for treating hyperlipemia, fragrant red clearing capsule, Tongbianling Capsules etc., the dregs of a decoction of the treatment hemorrhoid on Huang Including particularly good of curing haemorrhoids inflammation particle, hemorrhoid, the medicine (food) for filling blood, relaxing bowel with fungi include spot jade gill fungus, Awei mushroom, Black fungus, true pleurotus cornucopiae, Pleurotus tuber-regium etc.；The dispersing stagnated hepatoqi, the clearing heat and expelling damp Chinese patent drug dregs of a decoction include Longdan Xiegan wan, Yin Zhi Huang Grain, jaundice capillaris particle, nine taste Gantai capsules, nine taste Swertia patens capsules, liver-protecting tablet, legalon tablet, multiple happy JINDAN PIAN, liver health Rather, liver must answer, multiple liver energy, Yiganling tablet, the vigorous Yiganning capsule of liver, golden courage injection, callicarpa nudiflora, Golden Horse liver Thailand particle, hepatitis B Instant granule for curing, Yinzhihuang oral liquor, sharp diisopropyl amine dichloro acetate oral solution, cholagogic detoxicating capsule, Stop moving Mixture -I, hepatitis B strengthening capsule, hang down basin Careless electuary, hepatitis B strengthening capsule, Manganjieyu ' capsule, hepatitis B detoxifying capsule, Jianweiyuyang Tablets, callicarpa nudiflora, five Siberian cocklebur of oriental wormwood The dregs of a decoction of syrup, qizhi weitong granules, Danle capsule, lidan paishi tablets etc., the clearing heat and detoxicating, anti-inflammatory, invigorating spleen to remove dampness, liver protection The medicine (food) of protect liver includes Armilariella tabescens, rainbow conk, Poria cocos, Ganoderma Sinense, armillariella tabescens, agaricus bisporus, green mushroom, Antrodia camphorata, jade with fungi Rice black mould, grifola frondosus, Pleurotus nebrodensis, needle mushroom etc.；The tonifying kidney and strengthening yang, the Chinese patent drug or the health food dregs of a decoction of invigorate blood circulation include Nourshing kidney brain invigorating liquid, 18 tastes are tonified Qi of the kidney oral solution, beneficial age extract oral liquid, oral liquid of spring, GUIZHI FULING JIAONANG, seven ingredient beard beautifying The stupid pregnant health liquid of particle, Nuangongyunzi Capsule, TONGJINGBAO KELI, compound rehmannia kidney-tonifying wine, eight delicacies with motherwort cream, mulberry paste, prestige, Wen Jing Mixture, eucommia bark capsules, palace ring blood nourishing granule, " Jingwu " medical powder taken with water, paecilomyces hepiall chen, Gamboge bone-tonifying piece, east supernatural power capsule, cynomorium songaricum are mended Kidney capsules, the best's oral solution, tortoise deer oral liquor for spleen and kidney, Shenbao syrup, tonic tablet for kidney-reinforcing, the long spring capsule in the palace of the Qing Dynasty, Canegongbu Piece, three health-care capsules, Anti-coronavirus Antibody, woman's health treasured soft extracts, oral liquid of spring, zhuanggushenjin capsule, Huoxuetongmai capsules etc. The dregs of a decoction, the tonifying kidney and strengthening yang, healthcare medicine (food) with fungi include matsutake, hickory chick, cordyceps sinensis, gold oyster mushroom, pupa Cordyceps sinensis, Pleurotus tuber-regium schizophyllum commune etc.；The heat-clearing, promoting blood circulation, dissolving stasis, the gynaecology of analgesic and the cardiovascular disease Chinese patent drug dregs of a decoction include kind Navigate capsule, puerpera pacify capsule, FUKE QIANJIN PIAN, CHANFUKANG KELI, eight taste piece of Cortex Phellodendri, good of woman, 'Fuyankang ' tablet, woman's treasured particle, Red tablet, ten taste capsule of gynaecology, Chaoan piece, FUYANJING JIAONANG, Xinshenghua Chongji, leucorrhea disease treatment capsule, leukorrhea piece, control strap, Granule for treating gynecopathy changes disease Huisheng tablets, infusion of cherokee rose and spatholobus stem, Rhizoma Smilacis Bockii syrup, yimucao paste, TONGJINGLING CHONGJI, TONGJINGBAO KELI, basin inflammation only Piece, Duyiwei, return sheep particle, dysmenorrhoea capsule, eight taste piece of Cortex Phellodendri, FUKE ZHIDAI PIAN, coronary artery peaceful, coronary disease piece, vasculitis piece, The dregs of a decoction of cerebral ischemic, Xin Ke Shu ' tablet for treating coronary heart disease, pain-relieving and illness-eliminating capsule etc., the medicine (food) under the promoting blood circulation and hemostasis, anti-inflammatory dissolving stasis, uterine bleeding band are used Fungi includes that ergot, Pleurotus nebrodensis, red mushroom Phellinus, the red bacterium of bamboo, cepe, flesh ball fungus, black fungus, Uricularia polytricha, CNNS are small Skin umbrella, Lasiosphaera fenzlii, Trametes cinnabarina (Jacg) Franeh, Antrodia camphorata etc.；Clearing heat and detoxicating, pain, resolving sputum detumescence, the dispersing stagnated hepatoqi treatment mammitis of disappearing of invigorating blood circulation Chinese medicine slag includes YANKENING PIAN, Bazhen Pills, taraxacum weber, newborn sore ball, newborn disease spirit particle, 'rukuaixiao ' tablet, rupixiao tablets, rupixiao pian, the peaceful glue of breast The dregs of a decoction of capsule, Ruheneixiao granules, small Toxin-Vanquishing cream, Stasis-dissolving Capsule etc., the Chinese patent drug cinder ladle of the dredging collateral for promoting lactation include promoting lactation The dregs of a decoction of particle, the tool is clearing heat and detoxicating, the medicine (food) of detumescence and apocenosis function with fungi include Pleurotus tuber-regium, flesh ball fungus, pisolithus tinctorius Coker et Coucs, Trametes cinnabarina (Jacg) Franeh etc.；The dregs of a decoction of the bowl spares invigorating the spleen, enriching the blood and tonifying qi Chinese patent drug or health food include ginseng stilbene donkey-hide gelatin granule, ginseng stilbene It is male that piece, ginseng and pilose antler consolidate piece, four object mixture, eight delicacies cream, fine jade jade cream, blood nutritious instant granule, Xueshen liquid for oral administration, composite catechu capsulae, gold Oyster capsule, life-saving treasure capsule, Liuweidihuang Oral Liquid, QIJU DIHUANG KOUFUYE, ZHENQI FUZHENG CHONGJI, Radix Angelicae Sinensis jujube electuary, Ah Glue Aetanemic powder medicine, YANGXUE SHENGFA JIAONANG, Jian Nao Yi Zhi Instant Granules, YISHEN XIAOKE JIAONANG, yishenbugu oral solution, kidney-brain-nourishing tablet, The best's oral solution, Shenfu penetrate liquid, " Jingwu " medical powder taken with water, Xueshen liquid for oral administration, ZHENQI FUZHENG CHONGJI, return stilbene electuary, is eight delicacies cream, perfect big Tonic wine ball, GUHAN YANGSHENG JING, osmanthus dragon ointment, Liu-Wei-Bu, Shengxuebao-medicine for improving hematopoitic function particle, capsule for improving immunity, nourishing qi and blood oral solution, The dregs of a decoction of the rivers Zhong Hui yellow liquor, XINTONGKOU PIAN etc., the tonifying spleen appetizing, drugs for tonifying Qi and blood (food) include Uricularia polytricha, sulphur with fungi Sulphur bacterium, leopard mushroom, ustilago zeae, pholiota nameko, ganoderma lucidum, purple sesame, Wu Ling ginseng, Cordyceps militaris, true pleurotus cornucopiae etc.；The clearing heat and cooling blood solution The Chinese patent drug dregs of a decoction of poison include heat-clearing and detoxicating oral liquid, antiviral oral liquor, QINGKAILING KOUFUYE or injection, sweet dew removing toxic substances mouth It is blue to take liquid, compound infusion of isatis root, Injectio Folii Isatidis Composita, white tiger mixture, the anti-electuary of chaste tree, Yinhuang lung clearing capsule, compound plate Root electuary, cow-bezoar clearing brain tablet, cow-bezoar eight treasures (choice ingredients of certain special dishes) ball, rhinoceros are red, heat-clearing Inner disappears ball, curing toxin-dissipating pill, cornu bubali detoxifying pills, fire-clearing zhimai Piece, rhinoceros Dihuang Wan, multi-arch retaining structure, cool blood are brought down a fever the dregs of a decoction of antiphlogistic bolus, Compound Zhenzhu Jiedu oral liquid etc., the antibacterial Antiviral, the medicine (food) of cooling blood and hemostasis includes two types leather lid bacterium, foliosous parnassia herb, needle mushroom, cordyceps sinensis, ganoderma lucidum, Cinnamomum kanahirai hay with fungi Sesame, beef-steak fungus, mushroom, tabasheer, halimasch, grifola frondosus, east bolt bacterium, Cyathus striatus, Inonotus obliquus, calvatia gigantea, Luo Ba Dried mushroom etc.；The blood pressure lowering, the calming heart and tranquilizing mind of coronary heart disease include SHENQI WUWEIZI PIAN, Naolejing, Asclerol, just at the prescription dregs of a decoction Its ball, grows the heart-yin oral solution, Yening Tangjiang, Qiyeshen' and tablet, Anshen Bunao mixture, Anshen oral liquid, analmin at Tianmashouwu Tablets The nourishing blood and tranquilization such as syrup, sleeping Naolejing, Yangxue Anshen Syrup, sedative and heart-invigorating pill, ZAOREN ANSHEN YE, Qiyeshen' and tablet are grown Nourishing heart the moon Chinese medicine slag；Mountain chrysanthemum hypertension pill, Qingnao Jiangyapian, anti-hypertensive electuary, Fufang Danshen Pian, tranquilization paste, three health-care capsules, mountain chrysanthemum drop The dregs of a decoction of pressure, blood pressure lowering piece, QIANGLI NAOXINKANG JIAONANG, Naozhenning electuary, taste the heart-yin oral solution, naoliqing pill etc., the tool town Jing'an god, the medicine (food) that nourishing is calmed the nerves, calming heart is healthy and strong include fly agaric, Wu Ling ginseng, Cordyceps gunnii (Berk.) Berk, dictyophora phalloidea, Poria cocos etc. with fungi； The Chinese patent drug dregs of a decoction of the metabolic disease treatment include Hedan tablet, GUANSHU PIAN, Danshen Tablets, heart pellet living, infusion of fragrant solomonseal and hawthorn fruit, arteries and veins peace punching Agent, hawthorn lipid-lowering tablet, hypogastric lipid-lowering pill, anti-bolt BAOXIN PIAN, Kang Er heart-soothing capsules, diabetes pill, diabetes pill, Kelening capsule, golden stilbene The resolving phlegm lowering turbidities such as melbine, XIANLING GUBAO JIAONANG, strong kidney Dihuang Wan, activating blood and resolving stasis and treating hyperlipidemia, hyperglycemia, high cholesterol, Gui-shen pill, the desert cistanche of osteoporosis are good for the dregs of a decoction of kidney ball, bolus for invigorating kidney and improving ball, Compound Buguzhi granule etc., the hypoglycemic, blood Fat, cholesterol medicine (food) with fungi include vulcanized ester, tiger fur bacterium, halimasch, mushroom, pixie stool, grifola frondosus, dictyophora phalloidea, black ox Liver bacterium, Poria cocos etc.；The clearing and activating the channels and collaterals, wind-dispelling clearing damp treatment rheumatism bone disease Chinese patent drug cinder ladle include tendon-relaxing blood circulation-activating capsule, rheumatic bone Pain capsule, Fengshi maqian tablet, Summer interplanting cotton, turtle and Snake liquor, three snake medicinal liquors, guogong wine, Sanliangban Yaojiu, rheumatism wine, the pain of injury are peaceful Piece, exceedingly high oral solution, eliminating thrombus and removing obstruction in channels glue, the flat particle of radicular pain, sanqi shangyao tablets, sanqi shangyao pian, bone spur pain-eliminating piece, JINGFUKANG KELI, kanlisha coarse sand granules, kanlisha, NAODESHENG PIAN, Duyiwei, 'Gu Jin Wan ' capsule, Yushangling capsules, shangshi zhitong plaster, shangshi zhitong gao, Anyang refine cream, musk joint pain stopping The dregs of a decoction of cream etc., the promoting flow of qi and blood circulation, stagnation resolvation promote blood circulation treatment cardiovascular disease Chinese patent drug cinder ladle include LEMAI KELI, brain-benefiting blood pressure-reducing The peaceful treating coronary heart disease particle of piece, Tongxinluo medicinal capsule, Shuxin Oral, the heart, Diaoxinxue Kang oral solution, a continent heart can it is peaceful, Tai Ji is logical It takes orally the dregs of a decoction waited, and described promoting blood circulation and removing obstruction in channels, wind-expelling and dispelling dampness, the medicine to strengthen muscles and bones (food) includes CNNS's Marasmius, China with fungi Pleat pore fungi, halimasch, tabasheer, red mushroom, Qin mushroom, empty handle bolete etc.；The skin and mucosa inflammation disease Chinese patent drug cinder ladle includes CHUANXINLIAN JIAONANG, Sarcandra glaber dispersant tablets, cow-bezoar antiphlogistic tablet, xuanshi solution, jingwanhong soft plaster acne cream, jingwanhong soft plaster ointment, skin-haemorrhoids-clearing are soft Cream, tinosporae gel, capsule for treating piles with 9-medicinal herbs, Flos Chrysanthemi Indici granule, haemorrhoids bolt, detumescence hemorrhoids tablet, hemorrhoid pain peace liniment, treat hemorrhoid capsules, Zicao soft plaster, SHAOSHANG PENWUJI, ointment for treating burn and scald, pernio do not burst cream, complex rhizoma paridis tincture, jingwanhong soft plaster, sanhuang plaster, hinder furuncle cream, turtle shell disappears The dregs of a decoction of hemorrhoid capsule, compound capsule of natural indigo, kuh-seng bleb tincture etc., clearing heat and detoxicating, treatment carbuncle, subcutaneous ulcer, sore, furuncle, tinea the medicine (food) includes Trametes cinnabarina (Jacg) Franeh, Antrodia camphorata, Lasiosphaera nipponica(Kawam.)Y.Kobayasi, the brown pore fungi of coarse wool, trametes robinioplila etc. with fungi.

Preferably, the single Chinese herb residues are selected from the dregs of a decoction after the extraction of single Chinese herbal medicine；Other dregs of a decoction include swollen In dregs of a decoction and pill, powder, tablet of the Chinese patent drugs such as the tumor medication dregs of a decoction, ophthalmic remedy, ear nose larynx and Stomatological Preparations, acute disease etc. The dregs of a decoction in patent medicine preparation after the medicinal material extract of part；The antibacterial growth promotion fungi, which is selected from, to be grown fast protein content height and has antibacterial The medicine (food) that function is exempted from increasing uses bacterium or medicine (food) fungi same or similar with dregs of a decoction active compound function.Medicine food fungi bacteria includes medicinal true Bacterium, edible fungus and medicine-food two-purpose fungi, fermentation can be fermented with one or more mixing with Chinese herb residues；It also can be true with filiform Bacterium, probiotics mixing or step fermentation.

The invention will be further described below；

The method that the present invention carries out " four steps are preferred " combined fermentation：The selection of first step Chinese herb residues and prescription are (with other The dregs of a decoction or medicinal material are supplemented what is lacked in former prescription, and can be added and subtracted according to illness), according to prescription dregs of a decoction active compound The function and indication of material select the medicine food fungi bacteria to match to build fementative composition, orientation (function) fermentation are carried out, to mutually increase With complementary pharmacodynamic feature.Second step measures or solves the adaptability of fementative composition (can bacterium preferably grow)；Third walks, and measures The chief active functional component (component) of Zymocyte measures；4th step, feeding experiment verification the verifying results prepare particular functionality and raise Feed additives.

First, domestic most Chinese patent drugs (mainly liquid preparation, granule, paste, concentrated pill and tablet are selected Deng), the dregs of a decoction and powder of single Chinese herbal medicine and health products, pill, the dregs of a decoction in the preparations such as tablet after the medicinal material extract of part, by original The pharmacological property of medicinal material and function classification, according to animal different syndromes, (beast) medical knowledge opinion coaches in, to Chinese patent drug medicine Slag carries out selection and prescription, then the medicine (food) for selecting function same or similar builds fementative composition with fungi.

(1) Chinese medicine slag is classified：It is selected with the dregs of a decoction convenient for prevention Animal diseases, the country, which is produced the main Chinese patent drug dregs of a decoction, is divided into 8 19 group of major class, is specifically shown in Table 1.

(2) prescription：Under Chinese veterinarian's theory guidance, selected prescription slag, with single or herbal mixture slag or Chinese herbal medicine pair

It lacks the dregs of a decoction (individual or part medicinal material is un-extracted in side) supplement or carries out prescription adjustment：

1. supplement missing drug：To being compensated at scarce taste Chinese medicine in prescription slag, such as exploitation prevention liver and gall syndrome feed Additive selects nine taste Gantai capsules for the treatment of hepatitis, main component to have Radix Notoginseng, Radix Curcumae, centipede (not removing cephalopodium), rheum officinale (wine system), radix scutellariae, Chinese yam, puncture vine, turmeric, Schisandra chinensis totally nine taste, but wherein main ingredient Radix Notoginseng is directly used as medicine because crushing, without medicine Slag, so active compound should be supplemented or substitute medicine (madder, cattail pollen etc.) medicinal material or the dregs of a decoction.

2. the complementary function dregs of a decoction are added：The Chinese patent drug slag mixed fermentation of selection function complementation, such as exploitation treatment animal mamma as raw material Scorching feed addictive selects softening and resolving hard mass, promoting blood circulation to disappear bitterly, the clearing heat and detoxicating rupixiao tablets, rupixiao pian dregs of a decoction and nourishing qi and blood, dredging collateral for promoting lactation The granule for expelling obstruction of galactophore dregs of a decoction, the two mixed fermentation, clearing heat and detoxicating, dredging collateral for promoting lactation while swelling eliminating and pain stopping can accelerate mammitis Rehabilitation.Also clearing and activating the channels and collaterals, the Chinese patent drug slag of wind-dispelling clearing damp treatment rheumatism bone disease can promote blood circulation with the promoting flow of qi and blood circulation, stagnation resolvation treats painstaking effort The Chinese patent drug slag mixed fermentation of pipe disease, exploitation prevention animal rheumatic Bi syndrome feed addictive etc..

3. being added：The comprehensive additive of development function adds another reinforcement function on the basis of a certain Chinese patent drug slag The dregs of a decoction, such as feed addictive of exploitation prevention pneumonia selects the Chinese patent drugs such as Zhike Juhong Oral Liquid of clearing lung-heat, cough-relieving, resolving sputum The dregs of a decoction, clearing heat and detoxicating, the folk prescriptions or compound Chinese patent medicine of anti-inflammatory such as addition oral liquid of honeysuckle flower and scutellaria, CHUANXINLIAN JIAONANG, houttuynia cordata injection The dregs of a decoction, combined fermentation function is more complete, and effect is more preferable.The Chinese patent drug slag of heat-clearing and damp-drying drug treatment enteritis, diarrhea adds clearing heat and freeing strangury treatment urine The Chinese patent drug slag of calculus ferments, and can accelerate antidiarrheal；Also warm stomach invigorating the spleen, tonifying middle-Jiao and Qi Chinese patent drug slag add relieving diarrhea with astringents Chinese medicine or The dregs of a decoction can develop treatment and treat chronic stomach diarrhea feed addictive.

4. structure adjusting prescription：It selects a variety of dregs of a decoction prescriptions to ferment, middle mixture, digestion-promoting stomachic, Zhuo Dan compounds is built Ru small Help digestion the stomach strengthening and digestion promotings such as electuary, one kind of tonifying middle-Jiao and Qi Chinese patent drug slag or two kinds, and Xueshen liquid for oral administration, blood nutritious instant granule, compound is added to help The invigorating the bloods Chinese patent drug such as fragrant rattan mixture slag is a kind of or two kinds, then adds the tranquilizing the mind nourishing heart medium-height grass such as tranquilization paste, Naolejing, Yening Tangjiang One kind of the dregs of a decoction or two kinds of fermentations can develop aid digestion stomach invigorating, invigorating the blood, the feed addictive for promoting growth of animal.

(3) selection of the prescription dregs of a decoction and medicine food fungi bacteria fementative composition：According to the function and indication of prescription dregs of a decoction crude drug, It selects the medicine (food) that matches of function to build fementative composition with fungi, carries out orientation (function) fermentation, to enhance and mutually Mend pharmacodynamic feature.The Chinese medicine slag mycoplasma feed addictive of prevention animal anemofrigid cold is such as developed, selects wheat loose smut, hardship white The medicine (food) of the warm-natured sweating cold-dispelling such as hoof, bolete uses fungi.

Table 1 prevents animal different syndromes feed addictive Chinese herb residues and the combination of medicine/food fungi bacteria selects

Note：1, the above dregs of a decoction of all categories, can according to the characteristics of Animal diseases and the pharmacological property of crude drug, carry out splitting with Plus-minus is mixed with the single dregs of a decoction, a kind of prescription slag or multiple prescription slags, can also supplement Chinese medicine.

Secondly, measure or solve the adaptability of fementative composition (bacterium can preferably grow).The Chinese patent drug slag respectively selected, by true The requirement of bacterium fermentation preparation (mycoplasma) supplements lack part (in some Chinese patent drug prescriptions with other single dregs of a decoction (or medicinal material) Part medicinal material is directly entered patent medicine and does not leave the dregs of a decoction) or after making prescription plus-minus, drying and crushing adds the basis battalion of 10-30% Nutriment (agricultural and sideline products such as corn flour, bean cake powder, flour and mineral element somatomedin etc.) prepares fermentation substrate, after sterilizing, With hypha,hyphae strain 5-20%, (V/W, wherein V units are mL to the medicine (food) that inoculation is selected, and W units are g), to set suitable condition The growing state of mycelia is observed in lower culture, timing.The i.e. fementative composition that mycelia has grown is suitable.As the equal mycelia of survey strain does not give birth to It is long or grow bad, reason need to be searched, being such as certain taste Chinese medicine slag influences mycelia growth, and recombinant is fermented after rejecting；It can also incite somebody to action The compound dregs of a decoction first use filamentous fungi or probiotics fermention, afterwards with the method etc. of medicinal fungi fermentation, resolve the suitable of fementative composition Close sex chromosome mosaicism.

Third measures the chief active functional component (component) of Zymocyte.With comparison measuring, before measuring dregs of a decoction fermentation Main component situation of change afterwards：Nutrient component determining, polysaccharide determination, composition and effectiveness measures and bioactivity is (antibacterial, disease-resistant Malicious, immune, anti-inflammatory, analgesic etc.) measurement etc., to judge the substantial effect of fementative composition, it is final determine fementative composition and its The production technology of medicinal fungal substance.

4th, feeding experiment verifies the effect of combined fermentation medicinal fungal substance and prepared by functional feedstuff additive：

(1) feeding experiment：According to the pharmacodynamic feature of fermentation medicinal fungal substance, corresponding affected animal or morbidity cultivation are selected , it uses its medicinal fungal substance as feed addictive (or drug-medicinal fungal substance drug), carries out clinical test according to a conventional method, with Verify control effect.

(2) prepared by functional feedstuff additive：By the Chinese herbal medicine dregs fermented (medicinal fungal substance), direct dries pulverizing system Standby functional feedstuff additive；Add or be not added with 1-3%NaCl, protease, 37-50 DEG C, carry out under air-proof condition hyphal cell from After molten, dries pulverizing prepares functional feedstuff additive.

The present invention (beast) medical knowledge opinion in, by the Chinese medicine slag of different prescriptions using the property function of crude drug as foundation, into Row classifies and makees dregs of a decoction prescription according to animal difference syndrome.The medicine food fungi bacteria group of prescription Chinese medicine slag same or similar function Conjunction is oriented fermentation, produces the medicinal fungal substance feed addictive of specific function.The identical or different medicine food fungi bacteria of function is compound The function of Zymocyte is reinforced or expanded to fermented tcm slag；Medicine food fungi bacteria is with filamentous fungi, probiotic combination or substep to Chinese medicine Slag ferments, to overcome part Chinese medicine slag to be not suitable with medicine food fungi bacteria growth and breeding, non-orientable target；Or it is carried out after orientation fermentation Probiotic's culture, to enhance active function.The medicinal fungal substance of the fermentation dregs of a decoction uses self-dissolving technology row mycelium self-dissolving, improves nutrition The dissolution of substance and functional mass and utilization rate.The medicinal fungal substance of Chinese herbal medicine dregs orientation fermentation makees functional feedstuff additive, The chemicals such as substitute antibiotics are used for animal health and disease prevention and cure.

Compared with prior art, beneficial effects of the present invention are：

1, Chinese medicine slag carries out classification and prescription as Chinese medicine inferior (containing remaining pharmacological property ingredient) according to function and application, Fermentation, is improved and the content of transformation function ingredient makes functional feedstuff additive with potent function, is overcome previous By Chinese medicine slag simply as the low problem of industry byproduct utilization rate, the utilization rate and economic benefit of resource are greatly improved.

2, Chinese medicine slag and the same or similar medical edible fungal orientation fermentation of function realize the orientation conversion of functional component, Reach drug effect to be added or be multiplied, improves drug effect；Current Chinese medicine slag fermentation is overcome to be used as feed or feed addictive, it is main to consider battalion Value is supported, function fails to make full use of present situation, realizes that second of Chinese medicine slag efficiently utilizes.

3, with the micromycetes such as saccharomycete, probiotics elder generation fermented tcm slag, the object of the certain unfavorable medicine food fungi bacteria growths of conversion Matter, solves the problem on obstacle of medicine food fungi bacteria and part dregs of a decoction combined fermentation, this method system reports for the first time.

4, Chinese medicine slag orients solid fermentation mycoplasma, so that pharmacological property components Component is discharged through automyophagy method, improves its biology Availability, this is the new opplication of fungi self-dissolving technology.

5, orientation fermentation Chinese medicine slag prepares the feed addictive of specific function, the chemicals such as substitute antibiotics, and diseases prevention is controlled Disease carries out green health cultivation, and general feeds or feed addictive are used only as than the fermentation of previous Chinese medicine slag, be technology with theoretically Promotion.

6, this method is also applied for the development and application of cmm of simple prescription and compound preparation, is provided newly for the efficient application of Chinese herbal medicine Technical method.

Specific implementation mode

1 nine taste liver Thailand dregs of a decoction prescription biofermentations prepare the research of fish liver and gall syndrome prevention feed addictive

1.1 test method

(1) material：(prescription is Radix Notoginseng, Radix Curcumae, centipede, rheum officinale, radix scutellariae, Chinese yam, puncture vine, ginger to the nine taste Gantai capsule dregs of a decoction Yellow, Schisandra chinensis has disperse blood stasis and dredge collateral, soothing liver and strengthening spleen function)；Grifola frondosus, lark mushroom, agaricus bisporus, needle mushroom, Armilariella tabescens, rainbow conk, ox Antrodia prepares liquid spawn.

(2) method：1. dregs of a decoction compatibility and combined fermentation：Nine taste liver Thailand's dregs of a decoction are substituted with madder to be lacked in its prescription dregs of a decoction Radix Notoginseng, separately plus the 10-20% radix bupleuri dregs of a decoction dregs of a decoction are to enhance the function of dispersing stagnated hepatoqi, and drying and crushing adds basal nutrient material (corn Powder：Wheat bran：Bean cake powder is with 4：3：3 ratio dispensing, then 3%KH is added in mass ratio₂PO₄And 1.5%MgSO₄·7H₂O, mixing It forms；) 20% (W/W), mixing adds suitable quantity of water, prepares pharmacological property matrix, after high-temp sterilizing, is inoculated with respectively various medicinal true The growing state of mycelia is observed in bacterium liquid strain 10% (V/W), 26-28 DEG C of culture, timing.As a result Armilariella tabescens and Pleurotus nebrodensis are filtered out Two kinds of suitable strains for fementative composition.Again with the mixing (1 of this 2 plants of bacterium:1) bacterium fermentation pharmacological property matrix 3-4 days；In order to further Pharmacodynamic feature is improved, the medicinal fungal substance fermented adds the basal nutrient material of 5-10%, after high-temperature sterilization disinfection, then connects The mixing (1 of kind bacillus subtilis, brewer's yeast, bafillus natto, lactic acid bacteria bacillus and bacillus licheniformis etc.: 1:1:1:1) probiotics, 35-37 DEG C is cultivated 2 days.

2. effect measuring：Nutritional ingredient is measured with conventional method；Leaching liquor is measured with Odontothrips loti to imitate the inhibition of germ Fruit；To two mouthfuls of intensive fish ponds of fish liver and gall syndrome once occurred, the conventional feed not added is neutralized with 1% amount addition feed 50 days by a definite date comparison feeding experiments are carried out, control effect is measured.

1.2 test result：

(1) nutrient composition content measures：Medicinal fungal substance crude protein and roughly ash content, which have, significantly to be increased, increasing degree Respectively up to 46.35% and 43.55%；Crude fat, crude fibre decline, and fall is respectively 28.21%, 12.85%；It is various It is phosphorus 70.71%, manganese 36.31%, calcium 5.41%, magnesium 47.06%, iron that micronutrient levels, which has increase, increasing degree, 9.08%.It see the table below 2 and 3.

The conventional nutrients content (%) of table 2 nine safe capsule dregs of a decoction pharmacological property matrix and medicinal fungal substance

Table 4Routine nutrient contents of Chinese herb residues(CHR)and Fermented CHR (%)

The Mineral Concentrations (mg/kg) of table 3 nine safe capsule dregs of a decoction pharmacological property matrix and medicinal fungal substance

(2) Anti-bacterium performance measurement

5 kinds of pathogens of medicinal fungal substance pair have stronger fungistatic effect, inhibition zone straight in addition to weaker to streptococcus effect The big 1.2cm or more of diameter；Fungistatic effect further increases after probiotics fermention, the results are shown in Table 4.

Bacteriostatic test result (inhibition zone) unit for the medicinal fungal substance that table 4 ferments：cm

(3) clinical application effect measures：

Fish disease and dead fish phenomenon do not occur for experimental period test group fishpond；And control group fishpond July upper layer occur grass carp and The morbidity such as carp is dead, and sick fish is in that travelling is powerless, and feed intake is reduced, and occurs phenomena such as altering trip, dark floating head or spasm after ingesting.Solution Cut open disease fish and dead fish find the notable swelling of liver, quality become fragile, touch it is frangible；There are dotted or blocky bleeding or hemostasis or piebald liver； Gollbladder dilation or atrophy；The lesions such as bile color is dark green or blackish green.Then by 1% add the feed addictive feeding, the state of an illness by Step is controlled.

2 the best's oral solution Chinese medicine slag orientation fermentations prepare the research of functional feedstuff additive

2.1 test method：

(1) material:The best's oral solution dregs of a decoction (crude drug be Radix Angelicae Sinensis, radix rehmanniae preparata, Chinese yam, Radix Codonopsis, matrimony vine, mulberries, jujube, Radix Astragali, Os Bovis seu Bubali).Pleurotus tuber-regium, schizophyllum commune, Phellinus, hickory chick, matsutake prepare liquid spawn.

(2) method：1. fementative composition screens and technique：According to the method for example 1, the best's oral solution dregs of a decoction are added into basis Fuel (8:2) pharmacological property matrix is prepared, is inoculated with hickory chick, matsutake, ganoderma lucidum, Pleurotus tuber-regium, Pleurotus nebrodensis, Phellinus, schizophyllum commune, phoenix respectively The large-scale and small-sized filamentous fungi such as tail mushroom, root enzyme, trichoderma and aspergillus, cultivates 7 days, does not grow by 26-28 DEG C.In order to solve medicine With the culture problem of fungi, pharmacological property matrix uses geotrichum candidum, the first Candida of production, the button saccharomycete such as capsule yeast and brewer's yeast instead First to ferment, the mycoplasma fermented is smashed to pieces, is added 5% basal nutrient material to prepare pharmacological property matrix, is inoculated with medicinal fungi after sterilizing respectively, 26-28 DEG C, culture to mycelia is covered with；Growth selection is fast, and the strong medicinal fungi kind of pharmacological property is sent out as fementative composition bacterium with saccharomycete The best's Chinese medicine slag combined fermentation after ferment, then through drying, crushing, prepare functional feedstuff additive.2. effect measuring：Observation Method measures growing state of the medicinal fungi in saccharomycetes to make fermentation dregs of a decoction matrix；Conventional method measures medicinal fungi fermentation pharmacological property bacterium The nutrient composition content of matter changes；Odontothrips loti measures the Anti-bacterium effect of medicinal mycoplasma Aqueous extracts；Sulfuric acid-phynol method (measures Total reducing sugar) combining 3,5-dinitrosalicylic acids (DNS) colorimetric method (measuring reduced sugar) to measure polyoses content, (polyoses content=total reducing sugar contains Amount-content of reducing sugar)：Total saposins are measured with ultraviolet method (vanilla root rot colorimetric method for determining total saposins) to contain.3. selection 30 5-6 tires greatly enhance miscellaneous Suprapubic arch sling, are equally divided into the experiment of the fermentation dregs of a decoction, do not ferment 3 groups of dregs of a decoction control group and blank control, experiment 1% medicinal fungal substance additive (the pharmacological property yeast-like fungi matter of schizophyllum commune and Pleurotus tuber-regium mixing hair), control group addition 1% are added in group feed Do not ferment the dregs of a decoction, and blank control group is not added with former daily ration, carries out big perinatal period (pregnancy 90 days to weaned piglet, by a definite date 60 days) Feeding experiment, comparative measurements piglet growth with sow constitution situations such as.

2.2 test result：

(1) growing state of the food medicine fungi in yeast fermentation dregs of a decoction culture medium

For five kinds of macro fungis in addition to Phellinus, other 4 four kinds can be in the best's oral solution dregs of a decoction training after the fermentation of 3 primary yeasts It supports and is grown in base, especially schizophyllum commune and Pleurotus tuber-regium growth is best, and equal 48h mycelia is almost covered with；But it cannot be without yeast (being shown in Table 5) is grown on the Chinese medicine slag culture medium of bacterium fermentation.

Table 5：Growing state of the medicine food fungi bacteria in the mycoplasma culture medium for fermenting the best's oral solution dregs of a decoction through yeast

(2) fermentation medicinal fungal substance performance measurement

1. bacteriostatic test

The 3 kinds of fermentation dregs of a decoction 5 kinds of germs of extract pair, in addition to streptococcus, either Gram-negative bacteria or gram are positive Property bacterium fungistatic effect isocratic in having；And the dregs of a decoction extract that do not ferment is then without fungistatic effect.It the results are shown in Table 6.

Table 6：The bacteriostatic test result unit of the best's oral solution dregs of a decoction mycoplasma of secondary fermentation：cm

2. nutritional ingredient detects

Conventional nutrients：Saccharomycete and 3 kinds of medicinal fungis fermentation the best's dregs of a decoction, conventional nutrients have larger change Change, crude protein and crude fibre increase apparent, increase by 26.3%, 46.9%, 48.75% and 24.2% respectively, 47.0%, 60.2%；Crude fat declines, energy variation unobvious.It the results are shown in Table 7.

The conventional nutrients content (%) of 7 Chinese medicine slag of table and secondary fermentation Chinese medicine slag

Mineral element：Chinese medicine slag mineral element after Pleurotus tuber-regium and schizophyllum commune fermentation is changed significantly, wherein violent and magnesium increment Maximum the results are shown in Table 8 up to one times or one times or more.

The Mineral Concentrations (mg/kg) of 8 Chinese medicine slag of table and secondary fermentation Chinese medicine slag

Amino acid：Amino acid analysis is the results show that each amino acid is in that up-trend, especially schizophyllum commune are sent out after fermentation Amino acid increasing degree is maximum after ferment, and total amino acid content improves 52.8%, and wherein aromatic amino acid improves 98.7%, branch Amino acid 55.7%, linear chains of amino acids 32.7%, acidic amino acid 45.45, basic amino acid 61.6%, hydroxy-amino-acid 56.4%；The dregs of a decoction followed by after Pleurotus tuber-regium fermentation, the dregs of a decoction amino acid after matsutake fermentation change unobvious.It the results are shown in Table 9.

The amino acid content (%) of 9 Chinese medicine slag of table and secondary fermentation Chinese medicine slag

Polysaccharide is measured with saponin content

There is the contents of polysaccharide and saponin(e before 3 kinds of fermentation dregs of a decoction Zymocytes relatively ferment significantly increases, up to one times with On.It the results are shown in Table 10.

The polysaccharide of 10 Chinese medicine slag secondary fermentation mycoplasma of table and saponin content measurement result (3 average value)

(3) clinical application

The medicinal fungal substance to be fermented with Pleurotus tuber-regium and the dregs of a decoction that do not ferment are pressed in 1% amount addition feed, and feeding later stage of pregnancy is female Pig (antenatal 28 age in days of piglet in January-wean), three groups respectively feed 5-6 tires sow 10, as a result show：The either items of piglet The health indicator of index or sow, fermentation dregs of a decoction test group are significantly better than do not ferment dregs of a decoction control group, better than blank control Group (result is shown in 11).

The effect of the fermentation the best's dregs of a decoction feeding later stage of pregnancy and phase nursing period sow of table 11

3. the numb apricot Xuanfei granule dregs of a decoction of gold medal ear fermentation prepare the functional feedstuff additive of prevention livestock and poultry pneumonia

(1) fementative composition selects：The livestock and poultry respiratory inflammation mainly human body resistance caused by the causes of disease such as flu declines, disease It is in acute onset caused by the infection of bacterium virus, high fever, cough, abundant expectoration belong to phlegm-heat cough card, therefore select numb apricot Xuanfei granule more The dregs of a decoction make fermentation substrate.Dregs of a decoction system Chinese ephedra, semen armeniacae amarae, campanulaceae, fritillaria thunbergii, cordate houttuynia, honeysuckle, dried orange peel, the Radix Glycyrrhizae are through water There is ventilating the lung and relieving cough, the function of clearing heat and eliminating phlegm to be used for acute exacerbation of chronic bronchitis category phlegm heat-syndrome cough for residue after carrying, prescription It coughs card.Effective component is remained in the dregs of a decoction in order to give full play to, and reinforces its function, selects medicinal fungi gold ear.Tremella Warm-natured middle band is cold, sweet in flavor, and energy resolving sputum, cough-relieving, Dingchuan, regulating the qi flowing in the channels, soothing the liver intestines cure mainly lung heat, abundant expectoration, cold cough, asthma, high blood Pressure etc., similar to the numb function of apricot Xuanfei granule, to realize that function is identical, effect is added.

(2) combined fermentation is tested：Show that tremella can be well in numb apricot Xuanfei granule dregs of a decoction culture medium through preliminary test Middle growth and breeding then carries out formal test：By dregs of a decoction drying and crushing, 20% basal nutrient material is added by the method for example 1, Add suitable quantity of water, prepare pharmacological property matrix, be inoculated with 10% tremella fluid state strain, 26-28 DEG C ferments 3-4 days, waits for that mycelia is covered with.

(3) prepared by medicinal fungal substance feed addictive：The mycoplasma fermented soda solution tune PH8 is sealed at 40 DEG C Reason 12 hours, makes automyophagy, then 60-80 DEG C of forced air drying, is ground into fine powder, prepare medicinal fungal substance feed addictive.

(4) clinical application is tested：Certain cattle farm transports 60 8-12 monthly age calves back from the north, is divided to two cowshed raisings；Second Its beginning respectively has 1 ox morbidity, shows as loss of appetite, body temperature raising (40 DEG C), spiritual depressed, eye nose mucus sexual secretion, It is short of breath, pants, coughs, salivates.It is Mycoplasma bovis pneumonia that subsequent each cowshed has 2-3 ox morbidities, tentative diagnosis again, immediately Urgent epidemic prevention treatment, an injection tylosin 5mg/kg weight is taken to be used in conjunction 2 weeks two times a day；Another safe happy in injection While rhzomorph, 1% medicinal fungal substance additive is added in feed.As a result：There is 1 death in first group of first 3 hair disease, cuts open Inspection is in typical Mycoplasma bovis pneumonia lesion：Lung and the slight adhesion of pleura, there is a small amount of hydrops；Hydropericardium, liquid yellow clarification； The local red meat of apex pulmonis leaf and lobus cardiacus and part lobus diaphragmaticus becomes；There is suppuration stove to be dispersed in distribution simultaneously.Another 2 hair infected cattle still disappears It is thin, when have diarrhea, spiritual bad, continual cure.Second group of 4 hair infected cattle whole rehabilitations.

4. the trametes robinioplila Fermented Sausages health piece dregs of a decoction+Gegen Qinlian Tablets dregs of a decoction prepare prevention animal enterogastritis medicinal fungal substance feed addition Agent

(1) dregs of a decoction prescription and fementative composition：Acute gastroenteritis is the normal hair disease of animal, is invaded by germ, virus or toxin Caused by attacking, using diarrhea as cardinal symptom, doctor trained in Western medicine is treated with antibacterial, anti-inflammatory, antidiarrheal method, Chinese veterinarian thinks to catch cold, summer-heat and damp, Epidemic disease poison or internal injury diet injure stomach and intestine, caused by the wet transformation function of water is impaired, be divided into according to its cause of disease and symptom tremble with fear rush down, heat is rushed down, void is rushed down Equal diseases, but its base therapy principle is dampness removing and diarrhea-relieving.Therefore, the Gegen Qinlian Tablets dregs of a decoction (honey-fried licorice root, pueraria lobata, the coptis, radix scutellariae are selected Water carry residue), have heat-clearing and damp-drying drug, the function of expelling pathogenic factors from muscles and skin antidiarrheal；Add the intestines health piece dregs of a decoction (root of Aucklandia lappa Decne, evodia rutaecarpa water carry residue) have Fragrant qi-regulating, the function of antidiarrheal analgesic；The combination of two prescription slags plays the role of certain treatment enterogastritis.Select trametes robinioplila, foliosous parnassia herb, There is clearing heat and promoting diuresis, the medical edible fungal for the function that removes heat from the blood and relieve diarrhea to be combined fermenting and producing medicinal fungal substance for Pleurotus nebrodensis, 4 kinds of gloeostereum incarnatum, To enhance its function.

(2) fementative composition measures：According to the method for example 1, the dregs of a decoction crush, and add 10% basal nutrient material, prepare solid Body culture medium is inoculated with 10% different liquids strain respectively, and 28 DEG C are cultivated, the growing state of timing observation mycelia, as a result 4.1 kinds Bacterium can well grow on its culture medium.It the results are shown in Table 12.

Growing state of 12 different strain of table in compound dregs of a decoction culture medium

Note：+ to there is small bulk bacterium colony, ++ bacterium colony increases, +++ bacterium colony is in blocks, ++++mycelia is covered with

(3) fungistatic effect of combined fermentation medicinal fungal substance measures：According to the method for example 1, water extracts 4 kinds of medicinal fungal substances, Odontothrips loti measures inhibition of the extracting solution to pathogen.As a result it shows：4 kinds of medicine food fungi bacteria fermentation mixing dregs of a decoction mycoplasma extractions 5 kinds of germs of liquid pair all have a strong inhibitory effect, and the results are shown in Table 13

The bacteriostatic experiment unit of 13 medicine food fungi bacteria of table fermentation mixing dregs of a decoction extracting solution：cm

(4) medicinal fungal substance clinical application is tested：Medicinal fungal substance feed addictive is prepared with the compoiste fermented dregs of a decoction of gloeostereum incarnatum bacterium, One occurs pig epidemic diarrhea pig farm, selects 60 weanling pigs, divides three groups, every group 20, the 1st group (50mg/kg is sick with Western medicine Malicious azoles+gentamicin, intramuscular injection)；2nd group only, is fed with medicinal fungal substance drug, 10g/ with warm water mixing batter；3rd group, pharmacological property Mycoplasma 10g+ adjuvants 3g (activated carbon+artificial oral salt), is fed with warm water mixing batter；All treatment piglets are administered two times a day.Knot Fruit：It was treated through 5 days, the cure rate only 35% of Western medicine, effective percentage 65%；The cure rate 70% of medicinal fungal substance drug, it is efficient 90%；The cure rate of medicinal fungal substance+adjuvant is efficient up to 95% up to 75%.It is shown in Table 14.

Therapeutic test of the 14 compound dregs of a decoction Zymocyte of table to piglet epidemic diarrhea

Group

Piglet number

Cure rate %

Obvious effective rate %

Total effective rate %

Inefficiency %

Overall merit

Western medicine compares

20

35(7/20)

30(6/20)

65(2/20)

35(7/20)

It is poor

Medicinal fungal substance

20

70(14/20)

20(4/20)

90(18/20)

10(2/20)

Well

Medicinal fungal substance+adjuvant

20

75(15/20)

20(4/20)

95(19/20)

5(1/20)

It is outstanding

5. compound Chinese medicine slag orientation fermentation produces health care growth promoting biological feed additive

(1) dregs of a decoction and prescription：The Donky-hide blood-supplementing tonic dregs of a decoction (prepared rehmannia root, Radix Codonopsis, Radix Astragali, the fruit of Chinese wolfberry and Rhizoma Atractylodis Macrocephalae water/water Alcohol extracting residue), it is small build middle particle (Radix Paeoniae Alba, jujube, cassia twig, honey-fried licorice root, gingerade carry residue), five-medicine tranquilization particle (wheat, Pueraria lobata, jujube, Radix Glycyrrhizae, ginkgo leaf leaching residue) 3 kinds of dregs of a decoction, active compound synthesis has nourishing yin and supplementing blood, tonifying middle-Jiao and Qi, invigorating the spleen profit Lung, the work(of mental-tranquilization.

(2) fementative composition and growth measurement：Select ganoderma lucidum, purple sesame, the helps such as Cordyceps militaris to calm the nerves, nourishing and strengthening Yin and Yang it is medicinal true Bacterium and there are the fermentations in combination such as beneficial stomach, digestant foster tripe bacterium, measured through 28-30 DEG C of fermented and cultured, several bacterium can be compared with Good growth, 3-4 days mycelia can both cover with.

(1) combined fermentation effect measuring：

1. physics and chemistry is measured with nutritive index：According to a conventional method measure physical behavior, conventional nutrient, polysaccharide, content of peptides and To growth of probiotics breeding, (medicinal fungal substance adds 10% basal nutrient material to prepare culture medium, and inoculating lactic acid bacterium and clostridium butyricum are mixed Combined bacteria, 37 DEG C culture 12h, measure) influence.As a result it shows：Crude protein, amino acid, polysaccharide, content of peptides after several bacterium fermentations It increases substantially, crude fiber content declines, and the mycoplasma after fermentation is more suitable for growth of probiotics breeding.It the results are shown in Table 15.

Feed nutrition and functional component situation of change before and after 15 compound Chinese medicine slag combined fermentation of table

2. the influence to mouse immunity measures：Dregs of a decoction mycoplasma of fermenting is in 1% ratio addition daily ration, to feed mouse 14 It, measures influence of the mycoplasma to its immune function by standard method, is carried by a relatively large margin the results show that every immune indexes have It rises.It is shown in Table 16.

16 fermented tcm slag of table feeds influence of the mouse to immunity and measures

Note：※ hickory chicks and Ganoderma Lucidum each 5%.

(3) mixed fermentation Chinese medicine slag feeding cad pig experiment：

Certain pig farm select 20 because of the slow-growing wean weak pigle of disease (the white Daweizi pig of Yorkshire × length), with Machine is equally divided into 2 groups, respectively feeding experiment material (1% fermented tcm slag of addition) and to treatment (addition 1% do not ferment the dregs of a decoction), raises Feed phase 60d.As a result it shows：Stiff piglet feeding fermentation dregs of a decoction significant effect, with the dregs of a decoction ratio that do not ferment, daily gain improves 41.3%, Improve feed-weight ratio 16.3%.It is shown in Table 17.

The 17 mixed fermentation dregs of a decoction of table are to 60 days raising experiment results of sick and weak piglet

6. fermentation nourshing kidney brain invigorating liquid Chinese medicine slag prepares functional feedstuff additive

(1) combined fermentation：Nourshing kidney brain invigorating liquid Chinese medicine slag (fruit of Chinese wolfberry, the fruit of glossy privet, the fruit of Cherokee rose, Semen Cuscutae, raspberry, He Shou Crow, Radix Astragali, Chinese cassia tree, Radix Paeoniae Alba, Radix Glycyrrhizae, Radix Salviae Miltiorrhizae, Herba Epimedii water carry after residue) addition 20% basal nutrient material prepare fermentation base Matter prepares medicinal fungal substance drug with the Pleurotus tuber-regium of congenerous, schizophyllum commune fermentation.

(2) mycelium self-dissolving：By the medicinal fungal substance fermented with 1:Water is added in the material-water ratio of 3-5, and PH to 8 is adjusted in mashing, point It fills in 4 triangular flasks, wherein 3 are test group, adds 0.05% alkali protease, 2%NaCl, 0.05% alkali protease respectively + 2%NaCl；Another 1 not enzyme to compare with NaCl；Sealing, is handled, timing sampling sets microscopically observation in 40 DEG C of water-baths Mycelial cracking situation.

(3) important activity composition measurement：

1. measuring content and method

Polysaccharide determination：Sulfuric acid-phynol method (measuring total reducing sugar) combines 3,5-dinitrosalicylic acids (DNS) colorimetric method (to measure also Raw sugar) measure polyoses content (polyoses content=total sugar content-content of reducing sugar).

Determination of Total Flavonoids：Ultraviolet spectrophotometry (reference substance：Rutin Rutin).

2. inhibiting germ experiment：Inhibition of the medicinal fungal substance water extract to germ is measured with tablet-Odontothrips loti.

(4) result：

1. automyophagy is tested：As a result show that hypha,hyphae is under sealing, PH8,40 DEG C of water bath conditions in medicinal fungal substance, 4 It is small

When start self-dissolving, most of mycelia fragmentation in 8 hours, almost full fragmentation in 12 hours；Add alkali protease to mycelia from It is molten

There is certain facilitation, but not significantly；2%NaCL is added to have significant promotion self-dissolving.

2. important active substances measure：For determination of bioactive constituent the results show that after dregs of a decoction fermentation, polysaccharide and general flavone content are aobvious It writes and increases；Show that self-dissolving mycoplasma is in stronger inhibition to gram-negative Escherichia coli and Salmonella through bacteriostatic test Effect, inhibition zone 1.1-1.2cm the results are shown in Table 18

Polysaccharide, determination of total flavonoids and the fungistatic effect (3 average value) of 18 Chinese medicine slag Zymocyte of table

Note：* sulfuric acid-phynol method；※ UV spectrophotography (reference substances：Rutin Rutin)

(5) prepared by functional feedstuff additive：It dry, pulverize, pack under the conditions of 60-80 DEG C solvent of medicinal fungal substance, I.e. nourishing liver and kidney, the functional feedstuff additive of invigorating brain and relieving mental uneasiness can be used for growth promoting of fattening.

5.7 examples 7：Two kinds of single dregs of a decoction Fermentation Function feed addictive researchs

The radix bupleuri dregs of a decoction and astragalus root dregs add 20% basal nutrient material (corn flour respectively：Wheat bran：Bean cake powder is 4:3:3 Ratio dispensing, then in mass ratio add 3%KH2PO4 and 1.5%MgSO4.7H2O, mix；) pharmacological property matrix is prepared, it uses Medicine food fungi bacteria similar with its nature,taste and action is oriented fermentation, develops the medicinal fungal substance drug with crude drug function.

(1) the radix bupleuri dregs of a decoction：With clearing heat-fire, wind-dispelling heat-dissipating, prevention cat fever, the Pleurotus tuber-regium of cough, asthma, white ganoderma lucidum With Antrodia camphorata and its dregs of a decoction orientation fermentation, the novel mycoplasma drug of animal cold, fever can be prevented with exploitation；(resisted with clearing heat and detoxicating Bacterium is antiviral), hepatic cholagogic, adjust stomach delicious lactarius, hickory chick and Armilariella tabescens and its dregs of a decoction orientation fermentation, with develop can prevent The novel mycoplasma drug of animal disease in the liver and gallbladder.The results show that the extract of 6 kinds of macro fungi fermentation radix bupleuri dregs of a decoction mycoplasma is to sramana Salmonella has stronger inhibiting effect, has different degrees of inhibition to make Escherichia coli, staphylococcus, Pasteurella and streptococcus With；66.91% and 66.18% is respectively increased in delicious lactarius, toadstool ferment crude protein simultaneously, and corresponding amino acid increases substantially (about 2 times), crude fibre declines 36.88% and 29.10% respectively.This provides new mycoplasma for the prevention of flu or disease in the liver and gallbladder Drug (material).

(2) astragalus root dregs：With function it is similar (Buzhong, strengthening body, preventing phlegm from forming and stopping coughing, stomach invigorating antidiarrheal, detoxification apocenosis, Inducing diuresis for removing edema) Phellinus, white ganoderma lucidum, hickory chick and delicious lactarius be oriented fermentation, with develop it is novel have it is corresponding to Radix Astragali The medicinal fungal substance drug of function.The results show that the medicinal fungal substance of 4 kinds of medicine food fungi bacterias fermentation has the stronger work(for inhibiting germ Energy；26.40% and 13.23% is respectively increased in hickory chick, delicious lactarius ferment crude protein simultaneously, and crude fibre declines 25.35% He 40.11%, various amino acid contents increase considerably (about 1 times), enhance disease-resistant, health care, nourishing function.

Claims (6)

1. a kind of method that Chinese herb residues orientation fermentation prepares functional feedstuff additive, which is characterized in that the method be by Chinese herb residues are classified according to the pharmacological function of its crude drug, are selected for guidance with Chinese veterinarian's theory according to Animal diseases feature With prescription, then add basal nutrient material prepare druggability matrix, after sterilizing in chemotropism matrix inoculation with prescription dregs of a decoction pharmacology work( Same or analogous medicinal, the edible or medicine-food two-purpose hypha,hyphae strain of energy, obtains the medicinal fungal substance with specific function after fermentation, Up to functional feedstuff additive after dries pulverizing；In the pharmacological property matrix, the mass percent of basal nutrient material is 10- 30%；The inoculum concentration of the medicinal fungi hypha bacterial is 5-20%.

2. the method as described in claim 1, which is characterized in that the functional feedstuff additive includes anti-cures cold and influenza Feed addictive, anti-treating respiratory system disease feed addictive, prevent disease of digestive tract feed addictive, prevention reproduction The feed addictive of systemic disease, the feed addictive for preventing systemic disease, tranquilizing the mind and Metabolism regulation feed addictive, anti- Control the feed addictive of rheumatism and Wai Bing sections, somatotrophic feed addictive is exempted from the increasing of extra-nutrition antibacterial.

3. method as claimed in claim 2, which is characterized in that it is described flu with influenza include anemofrigid cold, anemopyretic cold, disease Toxicity is caught a cold；The respiratory disease includes acute inflammation, chronic inflammation；The disease of digestive tract include acute gastroenteritis, Chronic enterogastritis, constipation, liver and gall syndrome；The genital system diseases include Reproduction Disorder, postpartum disease, breast It is scorching；The systemic disease include deficient qi and blood disease, the infection of viral germ febrile illness；The tranquilizing the mind with Metabolism regulation includes Resisting stress adjusts metabolism；The rheumatism and Wai Bing sections include rheumatic Bi syndrome, surgery inflammation.

4. method as claimed in claim 3, which is characterized in that when preparing the feed addictive of prevention anemofrigid cold, Chinese herbal medicine Slag is selected from the relieving the exterior syndrome with drugs pungent in flavor and warm in property class Chinese patent drug dregs of a decoction for the treatment of anemofrigid cold, and fungi is selected from medicine/edible fungus of warm-natured sweating cold-dispelling； When preparing the feed addictive of prevention anemopyretic cold, Chinese herb residues are selected from the refrigerant inducing diaphoresis Chinese patent drug dregs of a decoction for the treatment of anemopyretic cold, Fungi is selected from medicine/edible fungus of dispelling wind and heat from the body inducing diaphoresis；When preparing the feed addictive of prevention virus flu, Chinese herb residues choosing From the clearing heat and detoxicating Chinese patent drug dregs of a decoction of ventilatining the lung and resolving phlegm, fungi is selected from clearing heat and detoxicating, phlegm-eliminiating and qi-regulating medicine/edible fungus；Prepare prevention When the feed addictive of acute respiratory inflammation, Chinese herb residues are selected from the dregs of a decoction of clearing heat, eliminating phlegm, relieving cough Chinese patent drug, and fungi is selected from and controls Medicine/edible fungus of cough with lung heat；Prepare prevention chronic airway inflammation feed addictive when, Chinese herb residues be selected from moistening lung, The dregs of a decoction of the Chinese patent drug of resolving sputum, cough-relieving, fungi are selected from tonifying Qi and blood, relieving cough and asthma medicine/edible fungus；Prepare prevention acute bowel When the feed addictive of gastritis, Chinese herb residues be selected from heat-clearing and damp-drying drug treatment enterogastritis Chinese patent drug slag, fungi be selected from clearing heat and promoting diuresis, Control medicine/edible fungus of dysentery；When preparing the feed addictive of prevention chronic enterogastritis, Chinese herb residues are selected from warm stomach invigorating the spleen, bowl spares QI invigorating, the Chinese patent drug slag of promoting digestion and removing indigestion, medicine/edible fungus that fungi is selected from invigorating the spleen to arrest diarrhea and stomach helps digestion；Prepare anti-treat constipation When feed addictive, Chinese herb residues are selected from clearing away heat and moistening the bowels, pleasant to lead stagnant Chinese patent drug slag, and fungi is selected from and fills blood, relaxes bowel Medicine/edible fungus；When preparing the feed addictive of prevention liver and gall syndrome, Chinese herb residues are selected from dispersing stagnated hepatoqi, clearing heat and expelling damp The Chinese patent drug dregs of a decoction, medicine/edible fungus of the fungi selected from clearing heat and detoxicating, anti-inflammatory, invigorating spleen to remove dampness, liver protecting；Prepare prevention breeding When the feed addictive of obstacle disease, Chinese herb residues be selected from tonifying kidney and strengthening yang, the Chinese patent drug or the health food dregs of a decoction of invigorate blood circulation, Fungi is selected from medicine/edible fungus of tonifying kidney and strengthening yang, healthcare；When preparing the feed addictive in prevention postpartum, Chinese herb residues choosing From heat-clearing, promoting blood circulation, dissolving stasis, the gynaecology of analgesic and the cardiovascular disease Chinese patent drug dregs of a decoction, fungi be selected from promoting blood circulation and hemostasis, anti-inflammatory dissolving stasis, Medicine/edible fungus under uterine bleeding band；When preparing the feed addictive of prevention mammitis, Chinese herb residues are selected from clearing heat and detoxicating, promoting blood circulation Analgesic, blood stasis dispersing and deswelling, dispersing stagnated hepatoqi treatment mammitis Chinese medicine slag and dredging collateral for promoting lactation Chinese patent drug slag, fungi be selected from tool heat-clearing solution Poison, medicine/edible fungus of detumescence and apocenosis function；When preparing the feed addictive of prevention deficient qi and blood disease, Chinese herb residues, which are selected from, to be mended Middle invigorating the spleen, enriching the blood and tonifying qi Chinese patent drug or health food the dregs of a decoction, fungi be selected from tonifying spleen appetizing, drugs for tonifying Qi and blood/edible fungus；System When the feed addictive of standby prevention febrile illness, Chinese herb residues are selected from the Chinese patent drug dregs of a decoction of clearing away heat,cooling blood and removing toxin, and fungi is selected from anti- Bacterium is antiviral, medicine/edible fungus of cooling blood and hemostasis；When preparing the feed addictive of prevention resisting stress, Chinese herb residues are selected from drop blood Pressure treats the calming heart and tranquilizing mind of coronary heart disease into the prescription dregs of a decoction, and fungi is selected from tool tranquilizing and allaying excitement, medicine/food that nourishing is calmed the nerves, calming heart is healthy and strong Use fungi；When preparing the feed addictive for adjusting metabolism, Chinese herb residues are selected from the Chinese patent drug dregs of a decoction of metabolic disease treatment, fungi Medicine/edible fungus selected from hypoglycemic, blood fat, cholesterol；When preparing the feed addictive of prevention rheumatic Bi syndrome, Chinese herb residues choosing From clearing and activating the channels and collaterals, wind-dispelling clearing damp treatment rheumatism bone disease Chinese patent drug slag and the promoting flow of qi and blood circulation, stagnation resolvation promote blood circulation treatment cardiovascular disease at The dregs of a decoction, fungi are selected from promoting blood circulation and removing obstruction in channels, wind-expelling and dispelling dampness, the medicine/edible fungus to strengthen muscles and bones；Prepare the feed addition of prevention surgery diseases When agent, Chinese herb residues are selected from the Chinese patent drug slag for the treatment of skin and mucosa disease, and fungi is selected from clearing heat and detoxicating, treatment carbuncle, subcutaneous ulcer, sore, furuncle, tinea Medicine/edible fungus；The somatotrophic feed addictive of extra-nutrition antibacterial is prepared, it is abundant to can be selected from single fiber content low nutrition Chinese herbal medicine or animals and plants extraction after residue or other prescription Chinese patent drugs the dregs of a decoction；The fungi is selected from the fast high protein of growth and contains Measure and have medicine/edible fungus of antibacterial functions or with the medicine/edible fungus same or similar with dregs of a decoction active compound function.

5. the method as described in claim 1, which is characterized in that Chinese medicine slag prescription includes Cheng Fangzhong classical or curative for effect It can be used corresponding single Chinese herb residues or Chinese patent drug containing single ingredient to supplement at the individual single Chinese herb residues lacked in the dregs of a decoction, also include phase Same or close pharmacological function Chinese medicine slag combination further includes plus taste prescription, the feed that such as preparation prevents anxious scorching respiratory tract disease adds When adding agent, the dregs of a decoction of heat-clearing poison Chinese patent drug can be added in the dregs of a decoction of clearing heat, eliminating phlegm, relieving cough Chinese patent drug；Prepare prevention chronic enterogastritis When feed addictive, warm stomach invigorating the spleen, tonifying middle-Jiao and Qi, can add in the Chinese patent drug slag of promoting digestion and removing indigestion the granatum of relieving diarrhea with astringents, myrobalan, The dregs of a decoction or crude drug of one or more of dark plum, nutmeg, red halloysite etc..

6. the method as described in claim 1, which is characterized in that medicine food fungi bacteria includes medicinal fungi, edible fungus and medicine food two With fungi, fermentation can be fermented with one or more mixing with Chinese herb residues；Also it can be mixed with filamentous fungi, probiotics or substep is sent out Ferment.