CN108432787B - Sterilization composition containing thiophanate methyl and thiason copper - Google Patents
Sterilization composition containing thiophanate methyl and thiason copper Download PDFInfo
- Publication number
- CN108432787B CN108432787B CN201810375812.2A CN201810375812A CN108432787B CN 108432787 B CN108432787 B CN 108432787B CN 201810375812 A CN201810375812 A CN 201810375812A CN 108432787 B CN108432787 B CN 108432787B
- Authority
- CN
- China
- Prior art keywords
- thiophanate methyl
- copper
- composition containing
- thieson
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a sterilization composition containing thiophanate methyl and thieson copper, and discloses a compound bactericide taking thiophanate methyl and thieson copper as active ingredients, which has obvious synergistic effect, delays the generation of key drug resistance, reduces the production cost and the use cost, and can be used for treating resistant diseases. Has better prevention and treatment effect on citrus sand skin diseases.
Description
Technical Field
The invention specifically relates to the technical field of pesticide compounding, and specifically relates to a bactericidal composition containing thiophanate methyl and thiasentong.
Background
Thiophanate methyl, which is a broad-spectrum systemic low-toxicity fungicide, has systemic, preventive and therapeutic effects. It was originally developed by Nippon Caoda corporation. Can effectively prevent and control diseases of various crops. The prevention and treatment of vegetable diseases can prevent and treat powdery mildew of cucumber, anthracnose, gray mold of eggplant, onion, celery, tomato, kidney bean and the like, anthracnose and sclerotinia, 1000-fold liquid of 50 percent wettable powder can be used for spraying for 1 time every 7 to 10 days and continuously spraying for 3 to 4 times at the initial stage of disease attack; can be used for preventing and treating lettuce gray mold and sclerotinia, and can be sprayed with 700 times of 50% wettable powder. The prevention and control of the flower diseases have certain prevention and control effects on dahlia flower rot, Chinese rose brown spot, Chinese flowering crabapple gray spot and kaffir lily leaf spot. In the initial stage of disease, 83-125 g of 50% wettable powder is used per mu, and is mixed with water for conventional spraying for 3-5 times.
Thiessen copper is thiazole organic copper bactericide, and is mainly used for preventing and treating rice bacterial leaf blight, bacterial leaf streak, cabbage soft rot and tomato bacterial wilt. Broad spectrum, low toxicity, safety, environmental protection, no public nuisance, special effect on bacterial diseases, high efficiency on fungal diseases, stability under acidic condition, and capability of being mixed with other pesticides.
Application of chemical agents is the most effective means of controlling plant diseases. However, continuous application of a single chemical fungicide in a high dose for a long period of time is likely to cause problems such as drug residue, environmental pollution, and development of drug-resistant fungi. The reasonable compounding or mixing of the chemical bactericide has the positive characteristics of expanding the bactericidal spectrum, improving the control effect, prolonging the application suitable period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the occurrence and the development of the drug resistance and the like of fungi, and the compounding of the bactericide is one of the most effective methods for solving the problems. The price of developing new products of bactericides is continuously rising, and compared with the price of developing and researching high-efficiency, low-toxicity and low-residue compounding and mixing, the bactericide has the advantages of low investment, short development period, attention paid to at home and abroad, and development strength are increased in many cases. On the basis of indoor screening and field tests, the thiophanate methyl and the thiessen copper are screened out for compounding, and the synergistic effect is obvious. And no report is made on the compounded sterilizing composition of thiophanate methyl and thiasentong and the application thereof at present.
Disclosure of Invention
In order to overcome the defects in the prior art, the sterilization composition containing thiophanate methyl and thiasentong is provided.
The technical solution adopted by the invention is as follows: the sterilizing composition is prepared by compounding thiophanate methyl and thiason copper, and the balance is auxiliary material components, wherein the mass ratio of the trifloxystrobin to the thiason copper is 1:40-40: 1.
The mass ratio of thiophanate methyl to thiasen copper is 1: 2.
The total mass of the thiophanate methyl and the thiasen copper accounts for 1-40% of the mass of the whole sterilization composition.
The total mass of the thiophanate methyl and the thiasen copper accounts for 30% of the mass of the whole sterilization composition.
The bactericidal composition is one of missible oil, a suspending agent, wettable powder, water dispersible granules, an aqueous emulsion, a microemulsion, granules and microcapsules.
The auxiliary material components comprise a dispersant, an antifreeze, a solvent, a synergist, a thickener, a defoamer and the like.
The invention has the beneficial effects that: the invention provides a sterilization composition containing thiophanate methyl and thieson copper, and the compound sterilization agent taking thiophanate methyl and thieson copper as effective components has obvious synergistic effect, delays the generation of key drug resistance, reduces the production cost and the use cost, and can be used for treating resistant diseases. Has better prevention and treatment effect on citrus sand skin diseases.
Detailed Description
The present invention will be described in detail with reference to examples, which are only preferred embodiments of the present invention and are not intended to limit the present invention.
The purpose of the test is as follows:
the method is entrusted by east wind chemical Co., Ltd, Zhejiang, and adopts a potted plant spraying method to test the indoor bacteriostatic activity of 3 medicaments of thiophanate methyl, thiasenone copper and thiophanate methyl, thiasenone copper (1:2) to the citrus sand skin disease; whether the medicaments have synergistic effect after being mixed is determined, and basis is provided for field test and further research and development.
Test conditions
Conditions of in vitro assay
Pathogenic bacteria to be tested: sand skin disease of citrus
Test bacterium culture conditions
A PSA culture medium, namely 20-25% of potatoes, 2% of cane sugar, 1.2% of agar powder and water, is adopted, and the pH value is natural. Peeling potato, cutting into small pieces, decocting in water bath for half an hour, filtering with double-layer gauze, adding sucrose and agar powder, dissolving, and diluting to desired volume. Subpackaging the flasks for 25-30 minutes at 121 ℃, autoclaving, pouring into plates with 10mL each, inoculating the test strain after solidification, and culturing in an incubator at about 25-26 ℃ for later use.
Instrumentation and equipment
The kit comprises a Sartorius BP211D 0.01.01 mg electronic balance, an eppendorf research 100-1000 mul pipette gun, a microscope, a pipette, a caliper, a puncher (the inner diameter is 5mm), a triangular flask, a measuring cylinder, a beaker, a plate, a stainless steel medicine spoon, a pressure steam sterilizer, an electrothermal blowing drying box, a sterile room, an incubator and the like.
Design of experiments
Reagent
Test agent
95% thiophanate methyl TC and 92% Thiessen copper TC (available from east China chemical Co., Ltd., Zhejiang)
Other reagents: n, N-dimethylformamide (purchased from shanghai reagent store).
Test treatment
Dose setting
Table 1 ex vivo test target trial dose design
The test was repeated: each treatment was set to 4 replicates.
Treatment method
Time of treatment
And (3) testing time: citrus sand skin disease: year 2018, month 4 and day 13.
Investigation time: citrus sand skin disease: year 2018, month 4 and day 16.
Device and method for administering medication
Pipette, punch (inner diameter 5mm), plate method
Test method
Referring to "indoor bioassay of pesticides test criteria bactericide part 2: bioassay is carried out by a method of a pathogenic fungus hypha growth inhibition test plate method (NY/T1156.2-2006): the test bacteria are inoculated on the prepared toxic medium, the diameter of the bacterial cake is 5mm, and then the bacterial cake is cultured in an incubator at about 24.3 ℃.
Calculation method
And calculating disease indexes and prevention and treatment effects of the treatments according to the survey data.
The disease index is calculated according to the formula (1), and the calculation result retains the last two decimal points:
in the formula: x-disease index; ni-number of diseased leaves at each level; i-relative grade value; n-survey total leaf number.
The prevention and treatment effect is calculated according to the formula (2):
in the formula: p-control effect, unit is percentage (%); CK-blank disease index; PT-index of disease treated by drug.
In vitro test investigation method
The investigation method comprises the following steps: after the culture in the incubator, the growth diameter (mm) of bacterial colonies is measured by a caliper, the diameter of each bacterial colony is measured by a cross method once, the average value is taken, and the diameter of the bacterial colony growth is calculated after the diameter of a bacterial cake is deducted.
Data statistical analysis:
and (3) calculating the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested according to the formula (1) and the formula (2) according to the investigation result, wherein the unit is percentage (%), and calculating the result which retains two decimal points.
D＝D1-D2·················(1)
In the formula: d, colony growing diameter; d1-colony diameter; d2-cake diameter.
I＝(D0-Dt)×100/D0···········(2)
In the formula: i-inhibition rate of hypha growth; d0-blank control colony growth diameter; dt-agent treatment colony growth diameter.
Statistical analysis
Performing regression analysis according to the log value of each agent concentration and the corresponding rate of incidence of hypha growth inhibition, and calculating EC of each agent50、EC9095% confidence limit. And calculating the co-toxicity coefficient (CTC) of the mixture according to the Sun Yunpei method. The CTC is more than or equal to 120, and the performance of the compound agent is enhanced; the compound agent with CTC less than or equal to 80 shows antagonism; the 80 < CTC < 120 combination showed additive performance.
Results and analysis
Calculating regression equation, correlation coefficient and EC from the inhibition rate of test agents including thiophanate methyl, copper thienyln, thiophanate methyl and copper thienyln (1:2) on citrus sand skin disease50And EC90A value; the specific results are shown in the following table 2, and the specific synergy coefficients are shown in the following table 3:
TABLE 2 results of thiophanate methyl, thiesencopper and thiesencopper (1:2) control of citrus sand disease
Table 2 sets forth the EC for the test target Citrus littoralis for the test agent50And calculating the co-toxicity coefficient of each mixed medicament to the corresponding test target, wherein the co-toxicity coefficient of each mixed medicament to the corresponding test target is shown in a table 8.
TABLE 3 synergistic effect of mixed medicines on corresponding test target plate
From table 3, it can be seen that the combination agents with synergistic effect are: thiophanate methyl: thiasen copper ═ 1:2 preventing and treating citrus sand skin disease.
Analysis and discussion of results
From the test results, thiophanate methyl: thiasen copper ═ 1:2 the prevention and treatment of citrus sand skin disease has synergistic effect.
Attaching original data
TABLE 4 thiophanate methyl, copper thienyls and thiophanate methyl, copper thienyls (1:2) results for control of citrus sand disease (3 days after application)
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (4)
1. An application of a bactericidal composition containing thiophanate methyl and thiasentong in preventing and treating citrus sand skin diseases is characterized in that: the sterilizing composition is prepared by compounding thiophanate methyl and thiason copper, and the balance is auxiliary material components, wherein the mass ratio of the thiophanate methyl to the thiason copper is 1: 2.
2. The application of the bactericidal composition containing thiophanate methyl and thieson copper in the prevention and treatment of citrus sand diseases according to claim 1, wherein the total mass of the thiophanate methyl and the thieson copper accounts for 1-40% of the total mass of the bactericidal composition.
3. The application of the bactericidal composition containing thiophanate methyl and thieson copper in the prevention and treatment of citrus sand diseases as claimed in claim 2, wherein the total mass of the thiophanate methyl and the thieson copper accounts for 30% of the total mass of the bactericidal composition.
4. The application of the bactericidal composition containing thiophanate methyl and thiesencopper in preventing and treating citrus sand skin disease according to claim 1, wherein the bactericidal composition is one of missible oil, a suspending agent, a wettable powder, a water dispersible granule, an emulsion in water, a microemulsion, a granule and a microcapsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810375812.2A CN108432787B (en) | 2018-04-25 | 2018-04-25 | Sterilization composition containing thiophanate methyl and thiason copper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810375812.2A CN108432787B (en) | 2018-04-25 | 2018-04-25 | Sterilization composition containing thiophanate methyl and thiason copper |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108432787A CN108432787A (en) | 2018-08-24 |
| CN108432787B true CN108432787B (en) | 2020-11-24 |
Family
ID=63201530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810375812.2A Active CN108432787B (en) | 2018-04-25 | 2018-04-25 | Sterilization composition containing thiophanate methyl and thiason copper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108432787B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110881476A (en) * | 2018-09-07 | 2020-03-17 | 刘力 | High-efficiency broad-spectrum low-toxicity environment-friendly agricultural new compound and composition thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292217A (en) * | 2000-11-21 | 2001-04-25 | 贾海燕 | Pesticide for controlling bacterial diseases of crops |
| CN101953346A (en) * | 2010-09-15 | 2011-01-26 | 浙江新农化工股份有限公司 | Zinc thiazole-containing bactericidal composition |
-
2018
- 2018-04-25 CN CN201810375812.2A patent/CN108432787B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292217A (en) * | 2000-11-21 | 2001-04-25 | 贾海燕 | Pesticide for controlling bacterial diseases of crops |
| CN101953346A (en) * | 2010-09-15 | 2011-01-26 | 浙江新农化工股份有限公司 | Zinc thiazole-containing bactericidal composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108432787A (en) | 2018-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102640761B (en) | Sterilization composition containing SYP-1620 and oomycete prevention and treatment sterilization agents | |
| CN110651790B (en) | Bactericidal composition for preventing and treating pineapple anthracnose | |
| CN104255757A (en) | Compound bactericide | |
| CN108432787B (en) | Sterilization composition containing thiophanate methyl and thiason copper | |
| CN107212011B (en) | A kind of bactericidal composition for preventing and treating crop disease | |
| CN109362759A (en) | A kind of bactericidal composition and its application of containing chlorine fluorine ether bacterium azoles and pyraclostrobin | |
| CN106719673A (en) | A kind of composition pesticide of containing fluopyram and chitosan | |
| CN108271798B (en) | Bactericidal composition containing carbendazim and thiasenc | |
| CN114946849B (en) | Medicament for preventing and treating powder Ge Jingji rot | |
| CN107333783A (en) | Application of the composition containing methyl jasmonate in citrus bacterial canker disease | |
| CN109548787A (en) | A kind of bactericidal composition and its application | |
| CN105746519B (en) | A kind of composition pesticide containing fenoxanil and lentinan | |
| CN108669080A (en) | A kind of bactericidal composition containing Boscalid and its application | |
| CN113207889A (en) | Bactericidal composition containing ethylicin and tebuconazole and application thereof | |
| CN103798262B (en) | A kind of bactericidal composition containing thiophanate-methyl and copper rosinate | |
| CN112715552A (en) | Bactericidal composition containing propionyl brassinolide and polyoxin and application thereof | |
| CN103814922B (en) | A kind of bactericidal composition containing carbendazim and copper rosinate | |
| CN109006833A (en) | A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease | |
| CN112042660A (en) | Application of tebuconazole and thienconzium containing compound bactericidal composition to prevention and treatment of wheat scab | |
| CN109006819A (en) | A kind of bactericidal composition and application thereof containing zoxamide Yu biphenyl pyrrole bacterium amine | |
| CN111374135B (en) | Compound composition of shenqinmycin and bitertanol and application thereof | |
| CN108522520B (en) | Bactericidal composition containing trifloxystrobin and Saisentong | |
| CN107156156A (en) | A kind of bactericidal composition | |
| CN107318856B (en) | Sterilization composition containing dichloro-oxazole and oxine-copper | |
| CN106342821A (en) | Prothioconazole- tebuconazole compounded suspending agent and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |