CN109006833A - A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease - Google Patents
A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease Download PDFInfo
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- CN109006833A CN109006833A CN201810998967.1A CN201810998967A CN109006833A CN 109006833 A CN109006833 A CN 109006833A CN 201810998967 A CN201810998967 A CN 201810998967A CN 109006833 A CN109006833 A CN 109006833A
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- CN
- China
- Prior art keywords
- fluazinam
- azophenlyene
- formamide
- sheath blight
- rice sheath
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
Abstract
The Synergistic complex and its application that the invention discloses a kind of for preventing and treating rice sheath blight disease, its compound is evaluated to the synergy potentiality of prevention and treatment Rhizoctonia solani Kuhn by mycelial growth rate method measurement azophenlyene -1- formamide (phenazine-1-carboxamide) and two kinds of medicaments of fluazinam (fluazinam), and using Wadley method.The result shows that: azophenlyene -1- formamide and fluazinam all have inhibiting effect to Rhizoctonia solani Kuhn.There is synergistic effect to Rhizoctonia solani Kuhn with 3:7 or 1:1 after two kinds of composite reagents, wherein best with 1:1 synergistic effect, coefficient of synergism SR is 1.85.In vitro to prevent and treat as the result is shown: azophenlyene -1- formamide and fluazinam are compounded to the control efficiency of rice sheath blight disease with 1:1 up to 82.89%, can be as the new medicament of prevention and treatment rice sheath blight disease.
Description
Technical field
The invention belongs to agriculture chemical compounding field, the synergy for relating generally to a kind of azophenlyene -1- formamide and fluazinam composition is multiple
With object, while being related to the compound and being applied in prevention and treatment rice sheath blight disease.
Background technique
Can rice be important cereal crops, keep the safety in production and affect the long-term stability of country.Rice is in production
In the process often by the interference of many factors, such as: temperature, illumination, nutrition, disease pest and weed etc., wherein rice sheath blight disease is to cause
One of an important factor for rice underproduction.The disease is the fungal disease as caused by Rhizoctonia solani Kuhn (Rhizoctoniasolani),
Rice base portion leaf sheath mainly is infected in rice tillering latter stage and Qi Sui latter stage, " moire shape " scab is formed, is caused every year by the disease
Production loss be 10-30%, it is serious up to 50%.
In production, the method for preventing and treating rice sheath blight disease mainly has cultural control, biological control, chemical prevention etc., wherein changing
Learning prevention and treatment is to control the important means of rice sheath blight disease.Currently, jinggangmeisu is always the important medicament for controlling rice sheath blight disease,
But there are the risks of drug resistance for long-term a large amount of applications.Prevent in some areas of China with the increase of dosage and times for spraying
Effect is begun to decline；In some countries of European Union, it is regarded as having risky drug and also starts to limit the use of.Therefore, prevention and treatment rice is sought
The new method of banded sclerotial blight is very urgent.
Two kinds of different medicaments of the mechanism of action are compounded, the service life of drug not only can be improved, but also can prolong
The drug resistance of slow germ.The result of study of this seminar early period shows that azophenlyene -1- formamide can pass through and inhibit Cell wall synthesis enzyme
Achieve the purpose that control pathogen growth with oxphos enzyme complex activity, and the mechanism of action of fluazinam is to block germ energy
(ATP) formation, since the two mechanism of action is different, and there is not yet the report that the two compounds, therefore, it is multiple that the present invention plans the two
Match, specify optimum formula and coefficient of synergism, it is intended to provide theoretical foundation for prevention and control rice sheath blight disease.
Summary of the invention
It develops drug resistance the technical problems to be solved by the present invention are: existing for Rhizoctonia solani Kuhn to chemical agent
Problem, provide it is a kind of for preventing and treating the Synergistic complex of rice sheath blight disease, it is multiple with two kinds of medicaments of azophenlyene -1- amide and fluazinam
Match, cannot be only used for prevention and treatment rice sheath blight disease, meanwhile, it also can effectively extend the service life of two kinds of medicaments.
In order to solve the above technical problems, the technical solution provided by the present invention is: a kind of for preventing and treating rice sheath blight disease
Synergistic complex, the compound are made of azophenlyene -1- formamide and fluazinam, the weight ratio of azophenlyene -1- formamide and fluazinam
For 3:7 or 1:1.
Preferably, the weight ratio of azophenlyene -1- formamide and fluazinam is 1:1 in the compound.
Present invention simultaneously provides application of the above-mentioned Synergistic complex in prevention and treatment Rhizoctonia solani Kuhn, by azophenlyene -1- first
Amide and fluazinam have synergistic effect, coefficient of synergism to the prevention and treatment of Rhizoctonia solani Kuhn with weight ratio 3:7 or 1:1 compounding
SR is respectively 1.77 and 1.85, thus, which can be prepared into the medicament of prevention and treatment rice sheath blight disease.
Azophenlyene -1- formamide provided by the invention and two kinds of composite reagent objects of fluazinam can expand antimicrobial spectrum, delay germ
Drug resistance reduces medicament residual.The research intends whether clear azophenlyene -1- formamide and fluazinam compounding have synergistic effect, purport
Theoretical foundation is provided in the new medicament of horn of plenty prevention and treatment rice sheath blight disease.
Specific embodiment
Technical solution of the present invention is described further below with reference to specific test and embodiment, present invention test and real
Applying percentage involved in example is mass percent.
The test material used in following embodiment:
Reagent agent: (95%, raw medicine is purchased from the Shanghai Qin to azophenlyene -1- formamide (phenazine-1-carboxamide)
Bar chemical inc)；(99.25%, raw medicine is purchased from the limited public affairs of the Shanghai Qin bar chemical industry share to fluazinam (fluazinam)
Department)；Jinggangmeisu (60%, be purchased from Kenuo Bio-Agricultural Chemicals Co Ltd, Wuhan)；Acetone, Tween-80, glucose, agar,
The medicaments such as NaOH, HCL are purchased from Chinese medicines group chemical reagents corporation.
Strains tested: Rhizoctonia solani Kuhn (Rhizoctonia solani), by plant protection institute of Agricultural University Of Hunan
Biological and ecological methods to prevent plant disease, pests, and erosion excavating resource and research on utilization room provide.
Culture medium: PDA culture medium (potato 200g, agar 20g, glucose 20g add water to be settled to 1000mL, and 121 DEG C
Sterilize 30min, and pH is natural).
The activation of strain: on aseptic working platform, PDA plate is made in PDA culture medium, the Rhizoctonia solani Kuhn of preservation is connect
In kind to PDA culture medium, it is placed in 25 DEG C of constant incubators and is inverted culture, it is spare.
Embodiment 1
1. indoor toxicity test
(1) pharmacy quality is arranged
In aseptic operating platform, reagent agent azophenlyene -1- formamide and fluazinam are used into a small amount of acetone organic solvent respectively
Dissolution, and it is configured to the mother liquor that mass concentration is 100 μ g/mL with sterile water, since different disease fungus is to different medicaments,
Mycelial growth rate is different, and every kind of medicament need to do preliminary experiment, 3 mass concentrations, 100,10,1 μ g/mL is arranged, is opposed with sterile water
According to, so that mass concentration range of the rate 10%~85% that be inhibited, sets 5 mass concentration gradients and is tested, for
Rhizoctonia solani Kuhn, the mass concentration of corresponding azophenlyene -1- formamide and fluazinam are as follows: 10,1,0.1,0.01,0.001 μ g/
mL。
EC of (2) two kinds of medicament mixtures to Rhizoctonia solani Kuhn50Value measurement
Proper amount of acetone is taken to dissolve azophenlyene -1- formamide and fluazinam raw medicine, being then prepared into concentration is 100 μ g/mL's
Mother liquor mixes azophenlyene -1- formamide and fluazinam according to the weight ratio of 1:9,1:4,3:7,2:3,1:1,3:2,7:3,4:1,9:1
Match, after being diluted with sterile water, by the volume ratio of medical fluid and PDA culture medium 1:9, is configured to drug containing tablet, while with clear water
It is set as no medicine plate and makees blank control, every group of processing sets 3 repetitions.With punch from 20 DEG C of left sides preculture 48h to 96h of temperature
Right colony edge is beatened to take bacteria cake, its face down is inoculated into drug containing tablet center, is placed in 20 DEG C of mold incubators, dark
After cultivating 72h (after at mycelia length to the 2/3 of plate), the colony diameter of each processing is measured using crossing method, is averaged
Afterwards, the bacteria cake diameter for subtracting 7mm is that bacterium colony increases diameter, calculates the inhibiting rate that medicament grows mycelia.Inhibiting rate (%)=
[(control bacterium colony increases diameter (mm)-processing bacterium colony and increases diameter (mm))/control bacterium colony increases diameter (mm)] × 100%；Bacterium
It falls and increases diameter (mm)=bacterium colony measurement diameter (mm)-bacteria cake diameter (mm).
It calculates the inhibiting rate of each concentration for the treatment of, then uses DPS software, calculate azophenlyene -1- formamide, fluazinam and its compound
Virulence regression equation, the EC of object50Value and related coefficient are finally adopted the synergy ratio method referring to Wadley, are answered different proportion
Co-toxicity evaluation is carried out with object, calculates coefficient of synergism SR.
Wadley method SR calculates as follows:
Note: EC50(ob)And EC50(th)Respectively represent practical EC50With theoretical EC50, A, B are single dose, and a, b are respectively that the two exists
The ratio of content in mixture, SR > 1.5 are synergistic effect, and 1.5 > SR > 0.5 are summation action, and SR < 0.5 is antagonism.
2. the compound of azophenlyene -1- formamide and fluazinam is to the inhibiting effect of Rhizoctonia solani Kuhn
The compound of azophenlyene -1- formamide and fluazinam is to the indoor toxicity determination test of Rhizoctonia solani Kuhn as a result, seeing
The following table 1.Wherein, 2 ratios have synergistic effect, be the weight ratio of azophenlyene -1- formamide and fluazinam are respectively 3:7 and 1:1,
Coefficient of synergism SR is respectively 1.77 and 1.85；The weight ratio of azophenlyene -1- formamide and fluazinam is 2:3 and 1:9, coefficient of synergism
SR is respectively 1.19 and 1.2, shows as summation action；The weight ratio of azophenlyene -1- formamide and fluazinam be 4:1,1:4,2:3,
9:1 and 7:3, coefficient of synergism SR are respectively 0.08,0.09,0.13,0.14,0.19, show as antagonism.
The indoor toxicity test of 1 azophenlyene -1- formamide of table and fluazinam compound to Rhizoctonia solani Kuhn
In vitro control efficiency of the compound of the present invention of embodiment 2 to rice sheath blight disease
It weighs azophenlyene -1- formamide and fluazinam raw medicine in the balance, is dissolved with proper amount of acetone, sterile water dilution is prepared into
500 μ g/mL mother liquors are spare.This test sets 4 processing altogether, 1 clear water control, i.e. azophenlyene -1- formamide processing (32 μ g/mL),
Fluazinam handles (0.14 μ g/mL), azophenlyene -1- formamide and fluazinam compound and handles (0.15 μ g/mL), comparison medicament Jing Gang
Mycin handles (recommended by routine Drug level) and clear water control treatment.The medical fluid prepared is uniformly applied to rice leaf sheath
On, dry Inoculated Rice sheath blight fungus, every processing is repeated 3 times, with smear sterile water for control, dry be placed on 20-23 DEG C it is black
Continuously cultivate 72h in dark culture case, using crossing method measure colony diameter, calculate control efficiency, control efficiency (%)=
((control scab length-processing scab length)/(control scab length-bacteria cake diameter)) × 100%, and it is soft using DPS6.55
Part analysis carries out variance analysis.
By the following table 2 it is found that azophenlyene -1- formamide and fluazinam compounding are most preferably reachable to the control efficiency of rice sheath blight disease
82.89%, it is secondly fluazinam, the control efficiency of other several medicaments is followed successively by jinggangmeisu and azophenlyene -1- first from big to small
Amide illustrates that azophenlyene -1- formamide and fluazinam compounding can further develop pesticidal preparations and can send out novel pesticide.
2 azophenlyene -1- formamide of table and fluazinam compounding are to the in vitro control efficiency of rice sheath blight disease (leaf sheath)
Note: T1: azophenlyene -1- formamide；T2: fluazinam；T3: azophenlyene -1- formamide and fluazinam answering with weight ratio 1:1
With object；T4: jinggangmeisu；CK: clear water control.
Claims (4)
1. a kind of for preventing and treating the Synergistic complex of rice sheath blight disease, which is characterized in that the compound by azophenlyene -1- formamide and
The weight ratio of fluazinam composition, azophenlyene -1- formamide and fluazinam is 3:7 or 1:1.
2. as described in claim 1 a kind of for preventing and treating the Synergistic complex of rice sheath blight disease, which is characterized in that the pheno
The weight ratio of piperazine -1- formamide and fluazinam is 1:1.
3. application of the Synergistic complex of any of claims 1 or 2 in prevention and treatment Rhizoctonia solani Kuhn.
4. application of the Synergistic complex of any of claims 1 or 2 in preparation prevention and treatment rice sheath blight disease medicament.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810998967.1A CN109006833A (en) | 2018-08-30 | 2018-08-30 | A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810998967.1A CN109006833A (en) | 2018-08-30 | 2018-08-30 | A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease |
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| Publication Number | Publication Date |
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| CN109006833A true CN109006833A (en) | 2018-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810998967.1A Withdrawn CN109006833A (en) | 2018-08-30 | 2018-08-30 | A kind of Synergistic complex and its application for preventing and treating rice sheath blight disease |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490557A (en) * | 2022-10-27 | 2022-12-20 | 中昇南阳生物科技有限公司 | Compound microbial fertilizer for preventing and treating rice sheath blight disease and preparation method thereof |
-
2018
- 2018-08-30 CN CN201810998967.1A patent/CN109006833A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490557A (en) * | 2022-10-27 | 2022-12-20 | 中昇南阳生物科技有限公司 | Compound microbial fertilizer for preventing and treating rice sheath blight disease and preparation method thereof |
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Application publication date: 20181218 |