CN106962377A - Compound Q uinolactacide application - Google Patents
Compound Q uinolactacide application Download PDFInfo
- Publication number
- CN106962377A CN106962377A CN201710301425.XA CN201710301425A CN106962377A CN 106962377 A CN106962377 A CN 106962377A CN 201710301425 A CN201710301425 A CN 201710301425A CN 106962377 A CN106962377 A CN 106962377A
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- compound
- uinolactacide
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- 0 C*(C(C1=CC=C*11C)=C2C1=O)c(cccc1)c1C2=O Chemical compound C*(C(C1=CC=C*11C)=C2C1=O)c(cccc1)c1C2=O 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses compound Q uinolactacide application.Shown in the structure of the compound such as formula (one), the compound Phytophthora infestans has preferable inhibitory activity, and MIC (MIC) is 8 μ g/mL, can be used as novel pesticide composition or lead compound with bactericidal action.The compound is microbe-derived natural products, and compared with traditional chemical synthesis bactericide, environment compatibility is good, and is easy to large-scale production.
Description
Technical field
The present invention relates to the application of microorganism source fungicide, specifically compound Q uinolactacide.
Background technology
The late blight of potato is also known as epidemic disease, potato pest, is to cause one kind that potato haulm is dead and stem tuber is rotten to be ruined
Going out property fungal disease, fall ill it is serious when, plant leaf wither it is vertical, crispatura, ultimately result in the black corruption of complete stool, cause potato Severe Reduction
Even have no harvest.The late blight of potato has generation in China potato major production areas, and loss is serious, and general time loss 30%~
50%, the time of being very popular even causes total crop failure.Current means of prevention relies primarily on the chemical synthesis agriculture such as thiophanate methyl, carbendazim
Medicine, but it is long-term a large amount of using having resulted in environmental pollution, the problems such as residues of pesticides are exceeded.With traditional chemical synthetic pesticide phase
Have that good to people and animals and non-target organism safety, environment compatibility, to be not likely to produce resistance etc. excellent than, microorganism source fungicide
Point.Therefore, the exploitation of microorganism source fungicide has with application to human health, environmental protection and the sustainable development of agricultural
Important meaning.
Compound Q uinolactacide shown in formula (one) is by Penicillium notatum Penicillium citrinum Thom F
1539 fermented cultures, extraction separate and obtain (Masaki Abe, Tetsuya Imai, Naoki Ishii, et
al.Quinolactacide,a new quinolone insecticide from Penicillium citrinum Thom
F 1539.Biosci.Biotechnol.Biochem.2005,69(6),1202-1205).Demonstrate,proved in the above documents by testing
It is bright：The compound has insecticidal activity to black peach aphid.
The content of the invention
The agriculture of preventing and treating phytophthora infestans is being prepared it is an object of the invention to provide compound Q uinolactacide
With the application in terms of bactericide.
To achieve these goals, the technical solution adopted by the present invention is：Compound Q uinolactacide application, institute
Stating compound Q uinolactacide Phytophthora infestans (Phytophthora infestans) has preferably suppression effect
Really, available for preparing disinfectant use in agriculture；Shown in the structure such as formula (one) of the compound Q uinolactacide.
Described disinfectant use in agriculture is：Using the compound shown in formula (one) as active component, what addition was agriculturally commonly used helps
Agent is prepared into wettable powder, granule or sustained release agent etc..
Advantage for present invention：Compound shown in formula (one) can be separated and obtained by the fermented culture of Penicillium notatum, extraction
, its Phytophthora infestans has preferable inhibitory activity, and MIC (MIC) is 8 μ g/mL, shows this
Quinolactacide compound Phytophthora infestans has preferable inhibition, can be as new with bactericidal action
Farm chemical ingredients or lead compound.
Quinolactacide compounds involved in the present invention can carry out scale fermentation using microorganism, with production
Technique is simple, and the cycle is short, the low feature of product cost；And carbon, three kinds of elements of hydrogen and oxygen are comprised only, it is easy to degraded in the environment,
It is relatively low to people and animals' toxicity.
Embodiment
Embodiment 1：Compound Q uinolactacide preparation
The preparation of compound Q uinolactacide shown in formula (one) is referring to document Masaki Abe, Tetsuya
Imai,Naoki Ishii,et al.Quinolactacide,a new quinolone insecticide from
Penicillium citrinum Thom F 1539.Biosci.Biotechnol.Biochem.2005,69(6),1202-
1205。
Embodiment 2：Bacteriostatic activity is tested
Using micro-dilution method, compound Phytophthora infestans (Phytophthora shown in formula (one) is determined
Infestans) bacteriostatic activity (《From natural products to New pesticides discovery-Principle Method》, Wu Wenjun, 2006, chemical industry goes out
Version society).
1) preparation of bacteria suspension
PDA culture medium surface will be inoculated in for examination fungi to cultivate after 72h in 28 DEG C, draw the sterile 0.85%NaCl of 2mL molten
Liquid (containing 0.25% Tween-20) washing culture, and gently scraped bacterium colony with glass slicker.Appropriate bacteria suspension is drawn in sterile
In test tube, 0.5 Maxwell is adjusted to than turbid (equivalent to 1.5 × 108CFU/mL it is) standby；
2) preparation of sample
1mg testing samples (formula (one) compound) are taken respectively, are dissolved in 100 μ L DMSO, after fully mixing, draw 50 μ
L sample solution is subsequently added into 50 μ L DMSO, obtains the sample solution that concentration halves into another centrifuge tube.According to this side
Method, obtains the sample solution that 12 groups of concentration halve successively.
3) MIC assay methods
(1) sterile working is used, the sample solution of various concentrations after doubling dilution is added separately to 96 sterile hole polyphenyl
In vinyl plate, the 1st to the 12nd hole respectively adds 5 μ L sample solution, and molten as blank control, plus 5 μ L DMSO to be not added with sample well
Fluid apertures is solvent control.
(2) the instruction bacteria suspension of 0.5 maxwell reduced turbidity is will be equivalent to, after diluting 1000 times through sabouraud culture medium, 95 μ L are taken
Be added sequentially in 96 orifice plates so that the sample concentration in the 1st to the 12nd hole is followed successively by 512,256,128,64,32,16,8,4,2,
1st, 0.5 and 0.25 μ g/mL.All of above sample is in triplicate.After gently concussion is mixed, 96 pore plate by sealing are placed in 28 DEG C of lifes
Change in incubator and cultivate 72h.
(3) light absorption value per hole is determined using ELIASA under 600nm wavelength, to completely inhibit indicator bacteria life in aperture
Long minimum sample concentration is the MIC of the compound.(note：When the obvious growth experiment of indicator bacteria is just intentional in negative control hole
Justice；When single jump hole occurs in experiment, the highest drug concentration for suppressing strain growth should be recorded；Such as there are many places and jump hole, then
Result should not be reported, need to repeat to test.)
Result of the test is：Compound Phytophthora infestans (Phytophthora infestans) shown in formula (one)
MIC value is 8 μ g/mL, with preferable bacteriostatic activity.
Above-mentioned the results show compound involved in the present invention has preferable bacteriostatic activity, available for preparing agricultural kill
Microbial inoculum.
Embodiment 3：Wettable powder
By the fermented cultures of Penicillium notatum Penicillium citrinum Thom F 1539, (fermented and cultured process can be found in
Above-mentioned document)；Zymotic fluid is subjected to press filtration and precipitation, by supernatant concentration, the content for making compound shown in formula (one) is 1.5-
2.0wt%, using concentrated supernatant quality as radix, adds 25-35% white carbons and is adsorbed, then add 3-8% dodecanes
Base sodium sulphate, 0.8-1.2%CMC and 2-4% pull open powders add attapulgite the content of compound shown in formula (one) are made and are
0.5wt% wettable powder.
Application method：500 times of liquid are sprayed on potato haulm.
Claims (2)
1. the compound Q uinolactacide shown in formula (one) is preparing the disinfectant use in agriculture side of preventing and treating phytophthora infestans
The application in face,
2. application as claimed in claim 1, it is characterized in that, the disinfectant use in agriculture is wettable powder, granule or sustained release
Agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710301425.XA CN106962377A (en) | 2017-05-02 | 2017-05-02 | Compound Q uinolactacide application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710301425.XA CN106962377A (en) | 2017-05-02 | 2017-05-02 | Compound Q uinolactacide application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106962377A true CN106962377A (en) | 2017-07-21 |
Family
ID=59330269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710301425.XA Pending CN106962377A (en) | 2017-05-02 | 2017-05-02 | Compound Q uinolactacide application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106962377A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623588A (en) * | 2017-03-24 | 2018-10-09 | 中国海洋大学 | A kind of tetracyclic and its preparation method and application containing lactams |
| CN108670970A (en) * | 2017-03-24 | 2018-10-19 | 中国海洋大学 | A kind of application of lactam analog compound in drug |
| CN108823108A (en) * | 2018-07-23 | 2018-11-16 | 山东省农业科学院蔬菜花卉研究所 | The superior strain and production method of industrialized production compound Q uinolactacide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005176684A (en) * | 2003-12-18 | 2005-07-07 | Otsuka Chemical Co Ltd | Oc45159 substance-productive fungus, method for producing the substance, and agricultural/horticultural insecticide |
| CN101691368A (en) * | 2009-08-25 | 2010-04-07 | 中国海洋大学 | Dehydration methylate of quinolinone alkaloid derivative and preparation method and application |
| CN103508950A (en) * | 2012-06-25 | 2014-01-15 | 南开大学 | 4-hydroxy-2-quinolinone compounds containing imine structure, and preparation and application thereof |
-
2017
- 2017-05-02 CN CN201710301425.XA patent/CN106962377A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005176684A (en) * | 2003-12-18 | 2005-07-07 | Otsuka Chemical Co Ltd | Oc45159 substance-productive fungus, method for producing the substance, and agricultural/horticultural insecticide |
| CN101691368A (en) * | 2009-08-25 | 2010-04-07 | 中国海洋大学 | Dehydration methylate of quinolinone alkaloid derivative and preparation method and application |
| CN103508950A (en) * | 2012-06-25 | 2014-01-15 | 南开大学 | 4-hydroxy-2-quinolinone compounds containing imine structure, and preparation and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| ABE, MASAKI 等: "Quinolactacide, a new quinolone insecticide from Penicillium citrinum Thom F 1539", 《BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623588A (en) * | 2017-03-24 | 2018-10-09 | 中国海洋大学 | A kind of tetracyclic and its preparation method and application containing lactams |
| CN108670970A (en) * | 2017-03-24 | 2018-10-19 | 中国海洋大学 | A kind of application of lactam analog compound in drug |
| CN108823108A (en) * | 2018-07-23 | 2018-11-16 | 山东省农业科学院蔬菜花卉研究所 | The superior strain and production method of industrialized production compound Q uinolactacide |
| CN108823108B (en) * | 2018-07-23 | 2021-06-25 | 山东省农业科学院蔬菜花卉研究所 | High-yield strain for industrially producing compound Quinolacctaside and production method |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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Application publication date: 20170721 |