CN107624767A - A kind of application of dipeptide compounds - Google Patents
A kind of application of dipeptide compounds Download PDFInfo
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- CN107624767A CN107624767A CN201710882783.4A CN201710882783A CN107624767A CN 107624767 A CN107624767 A CN 107624767A CN 201710882783 A CN201710882783 A CN 201710882783A CN 107624767 A CN107624767 A CN 107624767A
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Abstract
The invention discloses a kind of application of dipeptide compounds.Dipeptides (Dipeptides) the class compound has preferable inhibition to walnut anthrax bacteria (Gloeosporium fructigenum), MIC (MIC) is 64 μ g/mL, available for preparing disinfectant use in agriculture；Shown in the structure such as formula (one) of the Dipeptides classes compound.The compound belongs to microbe-derived natural products, is compared to traditional chemical synthesis bactericide, is easy to degrade in the environment, and be easy to large-scale production.
Description
Technical field
The present invention relates to microorganism source fungicide, specifically a kind of Dipeptides (dipeptides) class compound is answered
With.
Background technology
Walnut is long in China's cultivation history, a variety of micro- containing abundant protein and calcium needed by human, phosphorus, iron etc.
Secondary element, there is stomach invigorating, enrich blood, moistening lung, repose, promote longevity and other effects.With the gradual quilt of nutrition and medical value of walnut
People pay attention to, and its cultivated area is increasing, but the thing followed is that the generation of its disease is increasingly severe.Walnut anthracnose conduct
One of Major Diseases, there is the characteristics of incubation period is long, disease time is short, explosive strong, walnut maturation later stage fruit can be caused
A large amount of blackening, greatly reduce the yield of walnut.Means of prevention relies primarily on Bordeaux mixture, more good fortune MnZns, Tuzet, more at present
The chemical synthetic pesticides such as bacterium spirit, Bravo, although certain prevention effect can be obtained, it is also easy to bring environmental pollution, agriculture
The problems such as medicine residual is exceeded.Compared with traditional chemical synthetic pesticide, microorganism source fungicide has to people and animals and non-target
Bio-safety, environment compatibility is good, the advantages that being not likely to produce resistance.Therefore, the exploitation of microbe-derived agricultural chemicals and application are to people
Class health, environmental protection and the sustainable development of agricultural have great importance.
Dipeptides class compounds shown in formula (one) are to extract separation by Trichoderma tunning and obtain (E
Garo,CM Starks,PR Jensen,et al.Trichodermamides A and B,cytotoxic modified
dipeptides from the marine-derived fungus Trichoderma virens[J]
.J.Nat.Prod.2003,66,423-426), the document is experimentally confirmed：Trichodermamides B are anti-human in vitro
Colon cancer HCT-116 significant effect, IC50For 0.32 mcg/ml, the document does not disclose Trichodermamides A
And B other functions.
The content of the invention
It is an object of the invention to provide a kind of new opplication of dipeptides (Dipeptides) class compound.The present invention passes through experiment
Prove that it has preferable inhibitory activity to walnut anthrax bacteria, MIC (MIC) is 64 μ g/mL.
To achieve the above object, the technical solution adopted by the present invention is：A kind of dipeptides (Dipeptides) class compound
Using the Dipeptides classes compound has preferably suppression to walnut anthrax bacteria (Gloeosporium fructigenum)
Effect processed, available for preparing disinfectant use in agriculture；The structure of the Dipeptides classes compound is as shown in formula (one).
The formulation of the disinfectant use in agriculture can be wettable powder, granule or sustained release agent, with chemical combination shown in formula (one)
Thing is active component, adds agriculturally conventional auxiliary agent and is prepared.
Advantage for present invention：Compound shown in formula (one) can be separated and obtained by the fermented culture of Trichoderma, extraction
, it has preferable inhibitory activity to walnut anthrax bacteria, and MIC (MIC) is 64 μ g/mL, shows this
Dipeptides classes compound has preferable inhibition to walnut anthrax bacteria, can be as the novel pesticide with bactericidal action
Composition or lead compound.
Dipeptides classes compound involved in the present invention can carry out scale fermentation using microorganism, have production work
The features such as skill is simple, and the cycle is short, product cost is low；And 4 kinds of carbon, hydrogen, oxygen, nitrogen elements are comprised only, and it is easy to degrade in the environment, it is right
People and animals' toxicity is relatively low.
Embodiment
To illustrate the understanding to feature of present invention, the present invention is done into one with reference to some non-limiting embodiments
Step illustrates.
Embodiment 1：
Dipeptides class compounds shown in formula (one), it is Dipeptides class known compounds.Shown in formula (one)
The preparation process of Dipeptides class compounds can be found in document E Garo, CM Starks, PR Jensen, et
al.Trichodermamides A and B,cytotoxic modified dipeptides from the marine-
Record in derived fungus Trichoderma virens [J] .J.Nat.Prod.2003,66,423-426 is carried out.
Embodiment 2：Bacteriostatic activity is tested
Using micro-dilution method, compound is to walnut anthrax bacteria (Gloeosporium shown in measure formula (one)
Fructigenum) bacteriostatic activity (《From natural products to New pesticides discovery-Principle Method》, Wu Wenjun, 2006, chemical industry
Publishing house).
1) preparation of bacteria suspension
PDA culture medium surface will be inoculated in for examination fungi walnut anthrax bacteria (Gloeosporium fructigenum),
After 28 DEG C are cultivated 72h, the sterile 0.85%NaCl solution of 2mL (containing 0.25% Tween-20) washing culture is drawn, and use glass
Scraper gently scrapes bacterium colony.Appropriate bacteria suspension is drawn in sterile test tube, be adjusted to 0.5 Maxwell than it is turbid (equivalent to 1.5 ×
108CFU/mL it is) standby.
2) preparation of sample
1mg testing samples (formula (one) compound) and positive control (amphotericin B) are taken respectively, are dissolved in 100 μ L DMSO
In, after fully mixing, 50 μ L samples solution are drawn into another centrifuge tube, 50 μ L DMSO is subsequently added into, obtains concentration and halve
Sample solution.In this way, the sample solution that 12 groups of concentration halve successively is obtained.
3) MIC assay methods
(1) sterile working is used, the sample solution of various concentrations after doubling dilution is added separately to 96 sterile hole polyphenyl
In vinyl plate, the 1st to the 12nd hole respectively adds 5 μ L sample solution, and to be not added with sample well as blank control, adds 5 μ L DMSO molten
Fluid apertures is solvent control.
(2) the instruction bacteria suspension of 0.5 maxwell reduced turbidity is will be equivalent to, after sabouraud culture medium dilutes 1000 times, takes 95 μ L
Be added sequentially in 96 orifice plates so that the sample concentration in the 1st to the 12nd hole is followed successively by 512,256,128,64,32,16,8,4,2,
1st, 0.5 and 0.25 μ g/mL.All of above sample is in triplicate.After gently concussion mixes, 96 pore plate by sealing are placed in 28 DEG C of lifes
Change in incubator and cultivate 72h.
(3) using light absorption value of the ELIASA measure per hole under 600nm wavelength, to completely inhibit indicator bacteria life in aperture
Long minimum sample concentration is the MIC of the compound.(pay attention to：When the obvious growth experiment of indicator bacteria is just intentional in negative control hole
Justice；When single jump hole occurs in experiment, the highest drug concentration for suppressing strain growth should be recorded；Such as there are many places and jump hole, then
Result should not be reported, need to repeat to test.)
Result of the test is compound shown in formula (one) to walnut anthrax bacteria (Gloeosporium fructigenum)
MIC value is 64 μ g/mL, has preferable bacteriostatic activity.
Above-mentioned the results show compound involved in the present invention has preferable bacteriostatic activity, available for preparing agricultural kill
Microbial inoculum.
Claims (2)
1. application of the dipeptide compounds in terms of the disinfectant use in agriculture of preventing and treating walnut anthracnose is prepared shown in formula (one),
2. application as claimed in claim 1, it is characterized in that, the disinfectant use in agriculture is WP, granule or sustained release agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710882783.4A CN107624767A (en) | 2017-09-26 | 2017-09-26 | A kind of application of dipeptide compounds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710882783.4A CN107624767A (en) | 2017-09-26 | 2017-09-26 | A kind of application of dipeptide compounds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107624767A true CN107624767A (en) | 2018-01-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710882783.4A Pending CN107624767A (en) | 2017-09-26 | 2017-09-26 | A kind of application of dipeptide compounds |
Country Status (1)
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| CN (1) | CN107624767A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110558323A (en) * | 2019-08-30 | 2019-12-13 | 江苏大学 | dilysine/lycium barbarum polysaccharide synergistic antibacterial agent, and preparation method and application thereof |
| CN110558320A (en) * | 2019-08-30 | 2019-12-13 | 江苏大学 | Double-histidine/lycium barbarum polysaccharide composite antibacterial agent and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102159084A (en) * | 2008-07-17 | 2011-08-17 | 生物工厂公司 | Control of plant diseases and enhancing plant growth using a combination of a trichoderma virens species and a rhizosphere competent trichoderma harzianum species |
| CN103243029A (en) * | 2013-02-22 | 2013-08-14 | 中国水稻研究所 | Application of biocontrol fungus Trichoderma virens and metabolites thereof |
-
2017
- 2017-09-26 CN CN201710882783.4A patent/CN107624767A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102159084A (en) * | 2008-07-17 | 2011-08-17 | 生物工厂公司 | Control of plant diseases and enhancing plant growth using a combination of a trichoderma virens species and a rhizosphere competent trichoderma harzianum species |
| CN103243029A (en) * | 2013-02-22 | 2013-08-14 | 中国水稻研究所 | Application of biocontrol fungus Trichoderma virens and metabolites thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘淑宇 等: "绿色木霉菌发酵液对芒果采后炭疽病的抑制作用及保鲜效果", 《西北农林科技大学学报（自然科学版）》 * |
| 宋健 等: "天然产物Trichodermamide类似物的合成", 《精细化工》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110558323A (en) * | 2019-08-30 | 2019-12-13 | 江苏大学 | dilysine/lycium barbarum polysaccharide synergistic antibacterial agent, and preparation method and application thereof |
| CN110558320A (en) * | 2019-08-30 | 2019-12-13 | 江苏大学 | Double-histidine/lycium barbarum polysaccharide composite antibacterial agent and preparation method and application thereof |
| CN110558320B (en) * | 2019-08-30 | 2021-08-03 | 江苏大学 | Double-histidine/lycium barbarum polysaccharide composite antibacterial agent and preparation method and application thereof |
| CN110558323B (en) * | 2019-08-30 | 2021-09-10 | 江苏大学 | Dilysine/lycium barbarum polysaccharide synergistic antibacterial agent, and preparation method and application thereof |
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Application publication date: 20180126 |
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