CN108271798B - Bactericidal composition containing carbendazim and thiasenc - Google Patents
Bactericidal composition containing carbendazim and thiasenc Download PDFInfo
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- CN108271798B CN108271798B CN201810375783.XA CN201810375783A CN108271798B CN 108271798 B CN108271798 B CN 108271798B CN 201810375783 A CN201810375783 A CN 201810375783A CN 108271798 B CN108271798 B CN 108271798B
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- carbendazim
- bactericidal composition
- copper
- composition containing
- thiason
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a bactericidal composition containing carbendazim and Saisentong, and the compound bactericide taking the carbendazim and the Saisentong as effective components has obvious synergistic action, delays the generation of the drug resistance of the important factors, reduces the production cost and the use cost, and can be used for treating resistant diseases. Has better prevention and treatment effect on apple ring spot.
Description
Technical Field
The invention particularly relates to the technical field of pesticide compounding, and particularly relates to a bactericidal composition containing carbendazim and thiasencopper.
Background
Carbendazim is also named as cotton wilt and benzimidazole No. 44. Carbendazim is a broad-spectrum bactericide and has control effect on diseases of various crops caused by fungi (such as adelomycetes and ascomycetes). Can be used for foliar spray, seed treatment, soil treatment and the like. Can effectively prevent and treat various crop diseases caused by fungi, has wide application range in China, but the residue of the plant diseases can cause liver diseases and chromosome aberration and is toxic to mammals. Carbendazim is a high-efficiency low-toxicity systemic bactericide and has systemic treatment and protection effects. Can effectively prevent and treat various crop diseases caused by fungi, has wide application range in China, but the residue of the plant diseases can cause liver diseases and chromosome aberration and is toxic to mammals.
Thiessen copper is thiazole organic copper bactericide, and is mainly used for preventing and treating rice bacterial leaf blight, bacterial leaf streak, cabbage soft rot and tomato bacterial wilt. Broad spectrum, low toxicity, safety, environmental protection, no public nuisance, special effect on bacterial diseases, high efficiency on fungal diseases, stability under acidic condition, and capability of being mixed with other pesticides.
Application of chemical agents is the most effective means of controlling plant diseases. However, continuous application of a single chemical fungicide in a high dose for a long period of time is likely to cause problems such as drug residue, environmental pollution, and development of drug-resistant fungi. The reasonable compounding or mixing of the chemical bactericide has the positive characteristics of expanding the bactericidal spectrum, improving the control effect, prolonging the application suitable period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the occurrence and the development of the drug resistance and the like of fungi, and the compounding of the bactericide is one of the most effective methods for solving the problems. The price of developing new products of bactericides is continuously rising, and compared with the price of developing and researching high-efficiency, low-toxicity and low-residue compounding and mixing, the bactericide has the advantages of low investment, short development period, attention paid to at home and abroad, and development strength are increased in many cases. On the basis of indoor screening and field experiments, the carbendazim and the Saisentong are screened out for compounding, and the obvious synergistic effect is achieved. And no report is made on the compounded bactericidal composition of the carbendazim and the thiasenc and the application at present.
Disclosure of Invention
In order to overcome the defects in the prior art, the bactericidal composition containing trifloxystrobin and copper thiessen is provided.
The technical solution adopted by the invention is as follows: the bactericidal composition is prepared by compounding carbendazim and thiason copper, and the balance is auxiliary material components, wherein the mass ratio of trifloxystrobin to thiason copper is 1:40-40: 1.
The mass ratio of the carbendazim to the thiasen copper is 1: 2.
The total mass of the carbendazim and the thiasen copper accounts for 1-40% of the mass of the whole sterilization composition.
The total mass of the carbendazim and the thiasen copper accounts for 30% of the mass of the whole sterilization composition.
The bactericidal composition is one of missible oil, a suspending agent, wettable powder, water dispersible granules, an aqueous emulsion, a microemulsion, granules and microcapsules.
The auxiliary material components comprise a dispersant, an antifreeze, a solvent, a synergist, a thickener, a defoamer and the like.
The invention has the beneficial effects that: the invention provides a bactericidal composition containing carbendazim and Saisentong, and the compound bactericide taking the carbendazim and the Saisentong as effective components has obvious synergistic action, delays the generation of harmful drug resistance, reduces the production cost and the use cost, and can be used for treating resistant diseases. Has better prevention and treatment effect on apple ring spot.
Detailed Description
The present invention will be described in detail with reference to examples, which are only preferred embodiments of the present invention and are not intended to limit the present invention.
The purpose of the test is as follows:
entrusted by east China wind chemical Co., Ltd, Zhejiang, and a potted plant spraying method is adopted to test the indoor bacteriostatic activity of 3 medicaments, namely carbendazim, thiasenone, carbendazim and thiasenone (1:2), on apple ring spot; whether the medicaments have synergistic effect after being mixed is determined, and basis is provided for field test and further research and development.
Test conditions
Conditions of in vitro assay
Pathogenic bacteria to be tested: ring rot of apple
Test bacterium culture conditions
A PSA culture medium, namely 20-25% of potatoes, 2% of cane sugar, 1.2% of agar powder and water, is adopted, and the pH value is natural. Peeling potato, cutting into small pieces, decocting in water bath for half an hour, filtering with double-layer gauze, adding sucrose and agar powder, dissolving, and diluting to desired volume. Subpackaging the flasks for 25-30 minutes at 121 ℃, autoclaving, pouring into plates with 10mL each, inoculating the test strain after solidification, and culturing in an incubator at about 25-26 ℃ for later use.
Instrumentation and equipment
The kit comprises a Sartorius BP211D 0.01.01 mg electronic balance, an eppendorf research 100-1000 mul pipette gun, a microscope, a pipette, a caliper, a puncher (the inner diameter is 5mm), a triangular flask, a measuring cylinder, a beaker, a plate, a stainless steel medicine spoon, a pressure steam sterilizer, an electrothermal blowing drying box, a sterile room, an incubator and the like.
Design of experiments
Reagent
Test agent
98% carbendazim TC, 92% Saisentong TC (provided by east China chemical Co., Ltd., Zhejiang)
Other reagents: n, N-dimethylformamide (purchased from shanghai reagent store).
Test treatment
Dose setting
Table 1 ex vivo test target trial dose design
The test was repeated: each treatment was set to 4 replicates.
Treatment method
Time of treatment
Ring rot of apple: 3, 2018, and 30 months.
Investigation time: ring rot of apple: 4 and 3 months in 2018.
Device and method for administering medication
Pipette, punch (inner diameter 5mm), plate method
Test method
Referring to "indoor bioassay of pesticides test criteria bactericide part 2: bioassay is carried out by a method of a pathogenic fungus hypha growth inhibition test plate method (NY/T1156.2-2006): the test bacteria are inoculated on the prepared toxic medium, the diameter of the bacterial cake is 5mm, and then the bacterial cake is cultured in an incubator at about 24.3 ℃.
Data investigation and statistical analysis
Calculation method
And calculating disease indexes and prevention and treatment effects of the treatments according to the survey data.
The disease index is calculated according to the formula (1), and the calculation result retains the last two decimal points:
in the formula: x-disease index; ni-number of diseased leaves at each level; i-relative grade value; n-survey total leaf number.
The prevention and treatment effect is calculated according to the formula (2):
in the formula: p-control effect, unit is percentage (%); CK-blank disease index; PT-index of disease treated by drug.
In vitro test investigation method
The investigation method comprises the following steps: after the culture in the incubator, the growth diameter (mm) of bacterial colonies is measured by a caliper, the diameter of each bacterial colony is measured by a cross method once, the average value is taken, and the diameter of the bacterial colony growth is calculated after the diameter of a bacterial cake is deducted.
Data statistical analysis:
and (3) calculating the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested according to the formula (1) and the formula (2) according to the investigation result, wherein the unit is percentage (%), and calculating the result which retains two decimal points.
D＝D1-D2·················(1)
In the formula: d, colony growing diameter; d1-colony diameter; d2-cake diameter.
I＝(D0-Dt)×100/D0···········(2)
In the formula: i-inhibition rate of hypha growth; d0-blank control colony growth diameter; dt-agent treatment colony growth diameter.
Statistical analysis
Performing regression analysis according to the log value of each agent concentration and the corresponding rate of incidence of hypha growth inhibition, and calculating EC of each agent50、EC9095% confidence limit. And calculating the co-toxicity coefficient (CTC) of the mixture according to the Sun Yunpei method. The CTC is more than or equal to 120, and the performance of the compound agent is enhanced; the compound agent with CTC less than or equal to 80 shows antagonism; the 80 < CTC < 120 combination showed additive performance.
Results and analysis
Calculating regression equation, correlation coefficient and EC from the inhibition rate of tested medicaments carbendazim, thiaxon, carbendazim and thiaxon (1:2) on apple ring spot50And EC90A value; the specific results are shown in the following table 2, and the specific synergy coefficients are shown in the following table 3:
TABLE 2 carbendazim, Thiosensin and carbendazim-Thiosensin (1:2) results on prevention and treatment of apple ring rot
From Table 2, the EC of the test agent for the test target apple ring rot can be obtained50And calculating the co-toxicity coefficient of each mixed medicament to the corresponding test target, wherein the co-toxicity coefficient of each mixed medicament to the corresponding test target is shown in a table 8.
TABLE 3 synergistic effect of mixed medicines on corresponding test target plate
From table 3, it can be seen that the combination agents with synergistic effect are: carbendazim: thiasen copper ═ 1:2 preventing and treating apple ring spot.
Analysis and discussion of results
Carbendazim from the test results: thiasen copper ═ 1:2 has synergistic effect on preventing and treating apple ring rot.
Attaching original data
TABLE 4 carbendazim, Saisentong and carbendazim-Saisentong (1:2) results on apple ring spot disease control (4 days after application)
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (3)
1. The application of a bactericidal composition containing carbendazim and thiason copper in preventing and treating apple ring spot is characterized in that the bactericidal composition is prepared by compounding carbendazim and thiason copper, and the balance is auxiliary material components, wherein the mass ratio of the carbendazim to the thiason copper is 1: 2;
the total mass of the carbendazim and the thiasen copper accounts for 1-40% of the mass of the whole sterilization composition.
2. The application of the bactericidal composition containing the carbendazim and the thieson copper in the prevention and treatment of the apple ring spot as claimed in claim 1, wherein the total mass of the carbendazim and the thieson copper accounts for 30% of the total mass of the bactericidal composition.
3. The application of the bactericidal composition containing carbendazim and thiason copper in preventing and treating apple ring spot as claimed in claim 1, wherein the bactericidal composition is one of missible oil, suspending agent, wettable powder, water dispersible granule, emulsion in water, microemulsion, granule and microcapsule.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810375783.XA CN108271798B (en) | 2018-04-25 | 2018-04-25 | Bactericidal composition containing carbendazim and thiasenc |
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| CN108271798B true CN108271798B (en) | 2021-11-19 |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1109500C (en) * | 2000-11-21 | 2003-05-28 | 浙江东风农药厂 | Pesticide for controlling bacterial diseases of crops |
| CN101953346B (en) * | 2010-09-15 | 2011-07-20 | 浙江新农化工股份有限公司 | Zinc thiazole-containing bactericidal composition |
| CN102150687A (en) * | 2011-02-11 | 2011-08-17 | 吉林农业大学 | Method for preventing and curing ginseng diseases by mixing carbendazim with basic copper sulfate |
| CN104798798A (en) * | 2014-01-26 | 2015-07-29 | 沈阳化工研究院有限公司 | Binary composition and application thereof |
| CN103814922B (en) * | 2014-02-18 | 2015-09-30 | 中国水稻研究所 | A kind of bactericidal composition containing carbendazim and copper rosinate |
| CN106577706A (en) * | 2015-10-19 | 2017-04-26 | 重庆安尚园农业科技发展有限公司 | Combination of oxine-copper and bactericides for preventing and treating false smut and other fungi diseases |
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