CN108271798A - A kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene - Google Patents
A kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene Download PDFInfo
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- CN108271798A CN108271798A CN201810375783.XA CN201810375783A CN108271798A CN 108271798 A CN108271798 A CN 108271798A CN 201810375783 A CN201810375783 A CN 201810375783A CN 108271798 A CN108271798 A CN 108271798A
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- Prior art keywords
- thiophene
- carbendazim
- gloomy copper
- bactericidal composition
- composition containing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene, invention has apparent synergistic effect using carbendazim and the gloomy copper of thiophene as the composite bactericide of active ingredient, delay the generation of key drug resistance, and reduce production and application costs, can be used for the improvement of resistance disease.There is preferable control effect to ring rot of apple.
Description
Technical field
Present invention relates particularly to technical field of pesticide combination, and in particular to a kind of sterilization group containing carbendazim Yu the gloomy copper of thiophene
Close object.
Background technology
Carbendazim also known as carbendazol, carbendazol.Carbendazim is a kind of broad-spectrum germicide, to various crop by true
Disease has control effect caused by bacterium (such as Fungi Imperfecti, more sac fungus).It can be used for foliar spray, seed treatment and soil treatment
Deng.It can effectively prevent by fungus-caused various crop disease, the use scope in China is extensive, but its residual can cause liver
Disease and chromosome aberration, it is toxic to mammal.Carbendazim is high-efficiency low-toxicity systemic fungicide, has interior suction to treat and protect
Effect.It can effectively prevent by fungus-caused various crop disease, the use scope in China is extensive, but its residual can cause
Hepatopathy and chromosome aberration, it is toxic to mammal.
The gloomy copper of thiophene is thiazoles organic copper fungicide, mainly prevents bacterial blight of rice, bacterial stripe, the soft corruption of Chinese cabbage
Disease and bacterial wilt of tomato.High-efficiency broad spectrum, toxicity is low, safety and environmental protection, nuisanceless, to bacterial disease special efficacy, to fungal disease
Efficiently, stablize in acid condition, it can be with other combined use of pesticides.
It is the most effective means of controlling plant diseases using chemical agent.But it applies to long-term continuous high dose single
Chemical bactericide, be easy to cause the problems such as residual of medicament, environmental pollution and drug-resistant fungi develop.Rational chemistry
Bactericide compounded or mixture have expand fungicidal spectrum, improve control effect, extend the optimum period for applying fertilizer, reduce dosage, reduce poisoning,
The positive features such as residual, the generation for delaying drug resistance of fungi and development are reduced, bactericide compounded is to solve above-mentioned ask
One of the maximally efficient method of topic.Develop new product fungicide price is constantly soaring, and in contrast, exploitation with research efficiently,
Low toxicity, the compounding of low-residual and mixture have that small investment, the lead time is short and is paid attention to both at home and abroad, and increasing one after another develops
Dynamics.We filter out carbendazim and are compounded with the gloomy copper of thiophene based on the indoor screening and field trials, have apparent
Synergistic effect.And it has not been reported so far about the bactericidal composition and application of carbendazim and the compounding of the gloomy copper of thiophene.
Invention content
In order to overcome the problems of the above-mentioned prior art, a kind of Bactericidal composite containing trifloxystrobin Yu the gloomy copper of thiophene is provided
Object.
The technical solution that the present invention uses is：A kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene, it is described
Bactericidal composition is compounded by carbendazim and the gloomy copper of thiophene, remaining is adjunct ingredient, the quality of the trifloxystrobin and the gloomy copper of thiophene
Than being 1:40-40:1.
The mass ratio of the carbendazim and the gloomy copper of thiophene is 1:2.
The gross mass of the carbendazim and the gloomy copper of thiophene accounts for the 1-40% of entire Bactericidal composite amount of substance.
The gross mass of the carbendazim and the gloomy copper of thiophene accounts for the 30% of entire Bactericidal composite amount of substance.
The bactericidal composition be missible oil, suspending agent, wettable powder, water dispersible granules, aqueous emulsion, microemulsion,
One kind in granula, microcapsule formulations.
The adjunct ingredient is dispersant, antifreezing agent, solvent, synergist, thickener, antifoaming agent etc..
The beneficial effects of the invention are as follows：The present invention provides a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene, hair
It is bright that there is apparent synergistic effect as the composite bactericide of active ingredient using carbendazim and the gloomy copper of thiophene, delay the production of key drug resistance
It is raw, and production and application costs are reduced, it can be used for the improvement of resistance disease.There is preferable prevention to ring rot of apple
Effect.
Specific implementation mode
With reference to embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention,
It is not limitation of the invention.
Test objective：
It is entrusted by Zhejiang east wind Chemical Co., Ltd., using pot-spraying method, test carbendazim, the gloomy copper of thiophene, carbendazim
The gloomy copper of thiophene (1:2) Indoor antibacterial activity of 3 medicaments to ring rot of apple；Whether there is synergistic effect after determining pharmacy mix,
Foundation is provided for field trial and next step research and development.
Experimental condition
Isolated test condition
For trying pathogen：, ring rot of apple
For trying bacterium condition of culture
Using PSA culture mediums, i.e. potato 20~25%, sucrose 2%, agar powder 1.2% and water, pH are natural.By Ma Ling
Potato is removed the peel, and is cut into small pieces, is boiled in water-bath half an hour, and double gauze filtering adds sucrose, agar powder, constant volume after dissolving.Dispense triangle
Flask, 121 DEG C, high pressure sterilization 25~30 minutes is subsequently poured into plate, and per ware 10mL, access test bacteria strain, sets after solidification
It is spare after 25 DEG C~26 DEG C or so incubator cultures.
Instrument and equipment
Sartorius BP211D 0.01mg electronic balances, 100~1000 μ l liquid-transfering guns of eppendorf research,
Microscope, pipette, slide calliper rule, card punch (internal diameter 5mm), conical flask, graduated cylinder, beaker, plate, stainless steel medicine spoon, pressure are steamed
Vapour sterilizer, electric drying oven with forced convection, desinfection chamber, incubator etc..
Experimental design
Reagent
Test medicine
The gloomy copper TC of 98% carbendazim TC, 92% thiophene (offer of Zhejiang east wind Chemical Co., Ltd.)
Other reagents：N,N-Dimethylformamide (purchase of Shanghai reagent shop).
Test process
Dosage is arranged
Table 1 tests target test dose design in vitro
Experiment repeats：Each processing sets 4 repetitions.
Processing mode
Processing time
Ring rot of apple：On March 30th, 2018.
Control time：Ring rot of apple：On April 3rd, 2018.
Use instrument and administrated method
Liquid-transfering gun, card punch (internal diameter 5mm), Plating
Test method
Reference《Farm-chemical indoor determination test rule fungicide part 2：Inhibit disease fungus mycelia growth test flat
Ware method》The method of (NY/T 1156.2-2006) carries out bioassay：Inoculation test bacterium, bacterium i.e. on the toxic medium prepared
Cake diameter 5mm, is subsequently placed in 24.3 DEG C or so of incubator and cultivates.
Data survey and statistical analysis
Computational methods
According to survey data, the disease index and control effect of each processing are calculated.
Disease index is calculated by formula (1), and result of calculation retains 2 significant digits：
In formula：X-disease index；Ni-sick the numbers of sheets at different levels；I-is with respect to value of series；N-investigation total number of sheets.
Control effect is calculated by formula (2)：
In formula：P-control effect, unit are percentage (%)；CK-blank control disease index；PT-chemicals treatment disease
Feelings index.
Isolated test investigation method
Investigation method：It is measured with slide calliper rule after cultivating in the incubator and measures bacterium colony growth diameter (mm), each bacterium colony is used
Crossing method vertical measurement diameter is each primary, takes its average value, and the diameter that bacterium colony is grown is calculated after deducting bacteria cake diameter.
Data statistic analysis：
According to investigation result, each concentration for the treatment of is calculated to the mycelial growth inhibition rate for trying target bacterium by formula (1), (2),
Unit is percentage (%), and result of calculation retains 2 significant digits.
D=D1-D2·················(1)
In formula：D-bacterium colony increases diameter；D1- colony diameter；D2- bacteria cake diameter.
I=(D0-Dt)×100/D0···········(2)
In formula：I-mycelial growth inhibition rate；D0- blank control bacterium colony increases diameter；Dt- chemicals treatment bacterium colony increases straight
Diameter.
Statistical analysis
Make regression analysis according to each drug concentration logarithm and corresponding mycelial growth inhibition rate probit value, calculates each medicament
EC50、EC90, 95% confidence limit.The co-toxicity coefficient (CTC) of mixture is calculated according still further to Sun Yunpei's Method.The compounding agent tables of CTC >=120
Existing synergy；The compounding agents of CTC≤80 show antagonism；80 ＜ CTC ＜, 120 compounding agents show as being added.
As a result with analysis
From the gloomy copper of test medicament carbendazim, the gloomy copper of thiophene, carbendazim thiophene (1:2) inhibiting rate of ring rot of apple is calculated
Regression equation, related coefficient, EC50And EC90Value；Concrete outcome see the table below 2, and specific coefficient of synergism is shown in Table 3：
2 carbendazim of table, the gloomy copper of thiophene and the gloomy copper of carbendazim thiophene (1:2) ring rot of apple result is prevented
EC of the medicament to test target ring rot of apple can must be tested from table 250Value calculates each mixed medicament to accordingly testing
The co-toxicity coefficient of target, specific each mixed medicament are shown in Table 8 to the co-toxicity coefficient for accordingly testing target.
Table 3 uses medicament with and measures synergistic effect situation to accordingly testing target plate
The mixed medicament with synergistic effect is as can be seen from Table 3：Carbendazim：Gloomy copper=1 of thiophene：2 prevention apple wheel lines
Disease.
Interpretation of result and discussion
The carbendazim from the point of view of test result：Gloomy copper=1 of thiophene：2 prevention rings rot of apple have synergistic effect.
Attached initial data
4 carbendazim of table, the gloomy copper of thiophene and the gloomy copper of carbendazim thiophene (1:2) prevention ring rot of apple result (after medicine 4 days)
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene, which is characterized in that the bactericidal composition is by carbendazim
It is compounded with the gloomy copper of thiophene, remaining is adjunct ingredient, and the mass ratio of the trifloxystrobin and the gloomy copper of thiophene is 1:40-40:1.
2. a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
The mass ratio of carbendazim and the gloomy copper of thiophene is 1:2.
3. a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
The gross mass of carbendazim and the gloomy copper of thiophene accounts for the 1-40% of entire Bactericidal composite amount of substance.
4. a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene according to claim 3, which is characterized in that described
The gross mass of carbendazim and the gloomy copper of thiophene accounts for the 30% of entire Bactericidal composite amount of substance.
5. a kind of bactericidal composition containing carbendazim Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
Bactericidal composition is in missible oil, suspending agent, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations
One kind.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810375783.XA CN108271798B (en) | 2018-04-25 | 2018-04-25 | Bactericidal composition containing carbendazim and thiasenc |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810375783.XA CN108271798B (en) | 2018-04-25 | 2018-04-25 | Bactericidal composition containing carbendazim and thiasenc |
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| CN108271798A true CN108271798A (en) | 2018-07-13 |
| CN108271798B CN108271798B (en) | 2021-11-19 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292217A (en) * | 2000-11-21 | 2001-04-25 | 贾海燕 | Pesticide for controlling bacterial diseases of crops |
| CN101953346A (en) * | 2010-09-15 | 2011-01-26 | 浙江新农化工股份有限公司 | Zinc thiazole-containing bactericidal composition |
| CN102150687A (en) * | 2011-02-11 | 2011-08-17 | 吉林农业大学 | Method for preventing and curing ginseng diseases by mixing carbendazim with basic copper sulfate |
| CN103814922A (en) * | 2014-02-18 | 2014-05-28 | 中国水稻研究所 | Carbendazim and copper abietate containing bactericidal composition |
| CN104798798A (en) * | 2014-01-26 | 2015-07-29 | 沈阳化工研究院有限公司 | Binary composition and application thereof |
| CN106577706A (en) * | 2015-10-19 | 2017-04-26 | 重庆安尚园农业科技发展有限公司 | Combination of oxine-copper and bactericides for preventing and treating false smut and other fungi diseases |
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2018
- 2018-04-25 CN CN201810375783.XA patent/CN108271798B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292217A (en) * | 2000-11-21 | 2001-04-25 | 贾海燕 | Pesticide for controlling bacterial diseases of crops |
| CN101953346A (en) * | 2010-09-15 | 2011-01-26 | 浙江新农化工股份有限公司 | Zinc thiazole-containing bactericidal composition |
| CN102150687A (en) * | 2011-02-11 | 2011-08-17 | 吉林农业大学 | Method for preventing and curing ginseng diseases by mixing carbendazim with basic copper sulfate |
| CN104798798A (en) * | 2014-01-26 | 2015-07-29 | 沈阳化工研究院有限公司 | Binary composition and application thereof |
| CN103814922A (en) * | 2014-02-18 | 2014-05-28 | 中国水稻研究所 | Carbendazim and copper abietate containing bactericidal composition |
| CN106577706A (en) * | 2015-10-19 | 2017-04-26 | 重庆安尚园农业科技发展有限公司 | Combination of oxine-copper and bactericides for preventing and treating false smut and other fungi diseases |
Non-Patent Citations (2)
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| 吕国强等: "《果树主要病虫害识别与防治彩色图谱》", 30 November 2015, 河南科学技术出版社 * |
| 张纯标等: "新颖杀菌剂-噻森铜", 《世界农药》 * |
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