CN103798251B - A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene - Google Patents
A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene Download PDFInfo
- Publication number
- CN103798251B CN103798251B CN201410054435.4A CN201410054435A CN103798251B CN 103798251 B CN103798251 B CN 103798251B CN 201410054435 A CN201410054435 A CN 201410054435A CN 103798251 B CN103798251 B CN 103798251B
- Authority
- CN
- China
- Prior art keywords
- thiophene
- triazolone
- gloomy copper
- rice
- copper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Contain Recompounded pesticide and the application thereof of triazolone and the gloomy copper of thiophene, belong to technical field of compounded pesticide.It contains active ingredient triazolone and the gloomy copper of thiophene, and the weight ratio of described triazolone and the gloomy copper of thiophene is 1:10 ~ 10:1.The present invention is by triazolone and the gloomy copper of thiophene is composite significantly can expand fungicidal spectrum, thus to reach a spray how anti-or one spray the effect controlled more, thus saving manpower and pesticide cost, reduce the pollution to environment.In addition, because triazolone and thiophene gloomy copper mechanism of action is different, after two kinds of agriculture chemical compoundings, drug effect can produce synergistic effect, significantly improves control efficiency, and reduces the risk of pesticide resistance generation.
Description
Technical field
The invention belongs to technical field of compounded pesticide, be specifically related to a kind of Recompounded pesticide and the application thereof that contain triazolone and the gloomy copper of thiophene.
Background technology
Often there is multiple disease fungus, bacterial invasion in contemporaneity, the same position of crop, causes multiple diseases.As the rice tillering later stage often often leaf blast in rice and bacterial blight of rice occurs in leaf portion simultaneously; Rice green smut is roughly the same with rice leaf sheath rot proper control time; The diseases such as the withered and Rice Panicle maize ear rot of Rice blast, paddy bacterial paddy can be there is in rice earing stage fringe portion.The disease that contemporaneity occurs is infected caused by multiple fungi, the bacteriosis original often.These contemporaneities or (with) disease that occurs of same position likely solved by the method for exploitation Recompounded pesticide, namely reduces cost, improves drug effect, can prevent again peasant oneself mixture from causing poisoning and drug effect to decline.
Triazolone (Triadimefon), has another name called triadimefon, and its chemical name is 1 one (4 monochloro-benzene oxygen base) one 3,3 one dimethyl 1 (1H-1,2,4 one triazole-l-bases)-α-butanone, is the triazole bactericidal agent of the first extensive use.This bactericide has efficiently, low toxicity, low-residual, the lasting period is long, interior absorption is strong advantage, there is prevention simultaneously, treat, root out, fumigation action.Its mechanism of action mainly suppresses the synthesis of germ ergosterol, thus suppresses mycelial growth and sporulation.Its mechanism of action determines that it is effective to fungus disease, but to bacterium class disease effect extreme difference.
The chemical name of the gloomy copper of thiophene is N, N '-methylene-bis-(2-amino-5-sulfydryl 1,3,4 thiadiazoles) copper, belong to thiazoles and kill bacteria preparation, be made up of thiazole and copper ion, there is double sterilization mechanism.Thiazole has interior suction and therapeutic action to plant, has special efficacy to bacterial pathogen, the effect limited to fungi; Copper ion has prevention and protective effect, also has certain effect to bacterial disease.Two group actings in conjunction, better to the control efficiency of bacterial disease, fungicidal spectrum is wider, and efficiency time is longer, and sterilization mechanism is unique.But it is to the control efficiency of bacterial disease relatively, its chemical constitution determines it to fungus disease poor effect.
Summary of the invention
For prior art Problems existing, the object of the invention is to design provides a kind of containing triazolone and the Recompounded pesticide of the gloomy copper of thiophene and the technical scheme of application thereof.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that, containing active ingredient triazolone and the gloomy copper of thiophene, the weight ratio of described triazolone and the gloomy copper of thiophene is 1:10 ~ 10:1.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:9 ~ 9:1.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:4 ~ 1:1.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:1 ~ 9:1.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:4 ~ 2:3.
Described a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 3:2 ~ 4:1.
The described a kind of application of Recompounded pesticide in water prevention rice seed-borne disease containing triazolone and the gloomy copper of thiophene.
The described a kind of application of Recompounded pesticide in water prevention rice seed-borne disease containing triazolone and the gloomy copper of thiophene, is characterized in that described paddy rice seed-borne disease is Rice Panicle maize ear rot, rice blast, bakanae disease of rice, rice sheath blight disease, paddy bacterial brown spot, water Xanthomonas oryzae pv.oryzae, X. c. pv. oryzicola bacterium, Burkholderia glumae, rice green smut and rice leaf sheath rot.
The present invention is by triazolone and the gloomy copper of thiophene is composite significantly can expand fungicidal spectrum, thus to reach a spray how anti-or one spray the effect controlled more, thus saving manpower and pesticide cost, reduce the pollution to environment.In addition, because triazolone and thiophene gloomy copper mechanism of action is different, after two kinds of agriculture chemical compoundings, drug effect can produce synergistic effect, significantly improves control efficiency, and reduces the risk of pesticide resistance generation.
Embodiment
The present invention is further illustrated below in conjunction with test example.
Test example 1: triazolone and the composite Toxicity Determination to Rice Panicle maize ear rot pathogen of the gloomy copper of thiophene
1.1 medicament preparations
1.1.1 test medicine
The former medicine of 95% triazolone is produced by Jiangsu Jian Nong Chemical Co., Ltd..
The former medicine of the gloomy copper of 95% thiophene is by Zhejiang east wind Chemical Co., Ltd..
1.1.2 compounding method for medicament
Triazolone and the former medicine of the gloomy copper of thiophene to add respectively in sterile water and add a small amount of surfactant, through ultrasonic process, are mixed with suspension, and utilize sterile water to be diluted to 200mg/ml mother liquor respectively.Arrange according to mixture object and organize dosages distribution, 1:9,1:4,3:7,2:3,1:1,3:2,7:3,4:1,9:1 more, each proportioning is the mass ratio of two kinds of medicament active ingredients.
1.2 test strain
Rice Panicle maize ear rot pathogen bacterial strain: fusarium prolifertum
fusarium proliferatumfP1, Australian Bipolaris sacchari
bipolaris australiensisbA1, lunata
curvularia lunatacL1 and alternaria bacterium
alternaria tenuisaT1.
1.3 test method
Triazolone and the composite Toxicity Determination to Rice Panicle maize ear rot of the gloomy copper of thiophene adopt mycelial growth to suppress method to be carried out.
Under aseptic technique, according to test process, the sterilising medium melted in advance quantitative (being generally 60ml) is added in aseptic conical flask, from low concentration to high concentration, quantitatively draw liquid successively, add respectively in above-mentioned conical flask, fully shake up.Then equivalent pours more than 3 diameters into is in the culture dish of 9cm, and the pastille making respective concentration is dull and stereotyped.Test is established and is not made blank containing the process of medicament, and each process is no less than 3 repetitions.
The pathogen supported by medium, aseptically uses the sterilizing card punch of diameter 5mm, cuts bacterium cake from colony edge, and with inoculator, bacterium cake is connected to the dull and stereotyped central authorities of pastille, mycelia faces up, and covers ware lid, puts in the incubator of preference temperature and cultivates.
According to the growing state investigation pathogen mycelial growth situation of bacterium in blank culture dish.Use kind of calliper colony diameter, unit is millimeter (mm).Each bacterium colony right-angled intersection method vertical survey diameter is each once.
According to investigation result, utilize formula (1) and formula (2) to calculate each concentration for the treatment of to the mycelial growth inhibition rate for examination target bacterium, unit is percentage (%), and result of calculation retains 2 significant digits.
formula (1)
In formula:
-bacterium colony increases diameter;
-colony diameter;
-bacterium cake diameter.
formula (2)
In formula:
-mycelial growth inhibition rate;
-blank bacterium colony increases diameter;
-chemicals treatment bacterium colony increases diameter.
Utilize Tang Qi justice DPS DAS, the mycelial growth inhibition rate probability value according to each drug concentration logarithm value and correspondence does regression analysis, calculates each medicament.
1.4 composite effect assessments
At each single dose and built agent EC
50on the basis of value, calculate each built agent coefficient of synergism (SR) according to Wadley method, utilize SR value to evaluate composite effect.SR >=1.5 represent to have synergistic effect; SR≤0.5 represents to have antagonism; 0.5<SR<1.5 is summation action.
Coefficient of synergism (SR) calculates by formula (3) and formula (4):
formula (3)
In formula:
the theoretical value of-mixture, unit is milligrams per liter (mg/L);
the percentage composition of A in-mixture, unit is percentage (%);
the percentage composition of B in-mixture, unit is percentage (%);
The theoretical value of A in A-mixture, unit is milligrams per liter (mg/L);
The theoretical value of B in B-mixture, unit is milligrams per liter (mg/L).
formula (4)
In formula:
the coefficient of synergism of-mixture;
-mixture theoretical value, unit is milligrams per liter (mg/L);
-mixture measured value, unit is milligrams per liter (mg/L).
1.5 result of the test
According to above-mentioned test method, determine triazolone and thiophene gloomy copper single dose and each compounded formula to Rice Panicle maize ear rot four kinds of disease fungus bacterial strain fusarium prolifertums
f.proliferatumfP1, Australian Bipolaris sacchari
b.australiensisbA1, lunata
c.lunatacL1 and alternaria bacterium
a.tenuisthe suppression virulence of AT1 mycelia, has obtained the EC50 of each medicament to the suppression of four kinds of hypha,hyphaes respectively, and the method according to 1.4 obtains the EC50 value of each compounded formula, and result is as shown in table 1 ~ table 4:
Table 1 triazolone and thiophene gloomy copper single dose and each compound proportion are to the Toxicity Determination of sickle-like bacteria
| Reagent agent | Actual EC 50（ppm） | Theoretical EC 50(ppm) | Coefficient of synergism |
| Triazolone | 0.0567 | / | / |
| The gloomy copper of thiophene | 327.550 | / | / |
| Ketone thiophene proportioning 1:9 | 0.292 | 0.566 | 1.94 |
| Ketone thiophene proportioning 1:4 | 0.068 | 0.283 | 4.16 |
| Ketone thiophene proportioning 3:7 | 0.048 | 0.189 | 3.97 |
| Ketone thiophene proportioning 2:3 | 0.023 | 0.142 | 6.20 |
| Ketone thiophene proportioning 1:1 | 0.016 | 0.113 | 7.23 |
| Ketone thiophene proportioning 3:2 | 0.017 | 0.094 | 5.45 |
| Ketone thiophene proportioning 7:3 | 0.016 | 0.081 | 4.99 |
| Ketone thiophene proportioning 4:1 | 0.023 | 0.071 | 3.13 |
| Ketone thiophene proportioning 9:1 | 0.015 | 0.063 | 4.24 |
Table 2 triazolone and thiophene gloomy copper single dose and each compound proportion are to the Toxicity Determination of Bipolaris sacchari
| Reagent agent | Actual EC 50（ppm） | Theoretical EC 50(ppm) | Coefficient of synergism |
| Triazolone | 45.30 | / | / |
| The gloomy copper of thiophene | 240.450 | / | / |
| Ketone thiophene proportioning 1:9 | 62.555 | 168.053 | 2.69 |
| Ketone thiophene proportioning 1:4 | 33.523 | 129.164 | 3.85 |
| Ketone thiophene proportioning 3:7 | 26.522 | 104.891 | 3.95 |
| Ketone thiophene proportioning 2:3 | 21.021 | 88.298 | 4.20 |
| Ketone thiophene proportioning 1:1 | 14.512 | 76.237 | 5.25 |
| Ketone thiophene proportioning 3:2 | 12.402 | 67.075 | 5.41 |
| Ketone thiophene proportioning 7:3 | 15.836 | 59.880 | 3.78 |
| Ketone thiophene proportioning 4:1 | 15.507 | 54.078 | 3.49 |
| Ketone thiophene proportioning 9:1 | 17.778 | 49.301 | 2.77 |
Table 3 triazolone and thiophene gloomy copper single dose and each proportioning are to the Toxicity Determination of lunata
| Reagent agent | Actual EC 50（ppm） | Theoretical EC 50(ppm) | Coefficient of synergism |
| Triazolone | 7.21 | / | / |
| The gloomy copper of thiophene | 225.76 | / | / |
| Ketone thiophene proportioning 1:9 | 21.242 | 56.003 | 2.64 |
| Ketone thiophene proportioning 1:4 | 6.339 | 31.966 | 5.04 |
| Ketone thiophene proportioning 3:7 | 3.540 | 22.367 | 6.32 |
| Ketone thiophene proportioning 2:3 | 2.671 | 17.201 | 6.44 |
| Ketone thiophene proportioning 1:1 | 2.116 | 13.974 | 6.60 |
| Ketone thiophene proportioning 3:2 | 2.136 | 11.766 | 5.51 |
| Ketone thiophene proportioning 7:3 | 3.032 | 10.161 | 3.35 |
| Ketone thiophene proportioning 4:1 | 3.086 | 8.941 | 2.90 |
| Ketone thiophene proportioning 9:1 | 3.114 | 7.983 | 2.56 |
Table 4 triazolone and thiophene gloomy copper single dose and each proportioning are to the Toxicity Determination of alternaria bacterium
| Reagent agent | Actual EC 50（ppm） | Theoretical EC 50(ppm) | Coefficient of synergism |
| Triazolone | 64.26 | / | / |
| The gloomy copper of thiophene | 276.480 | / | / |
| Ketone thiophene proportioning 1:9 | 92.932 | 207.840 | 2.24 |
| Ketone thiophene proportioning 1:4 | 45.086 | 166.504 | 3.69 |
| Ketone thiophene proportioning 3:7 | 40.433 | 138.882 | 3.43 |
| Ketone thiophene proportioning 2:3 | 23.219 | 119.121 | 5.13 |
| Ketone thiophene proportioning 1:1 | 18.220 | 104.282 | 5.72 |
| Ketone thiophene proportioning 3:2 | 26.813 | 92.731 | 3.46 |
| Ketone thiophene proportioning 7:3 | 26.577 | 83.484 | 3.14 |
| Ketone thiophene proportioning 4:1 | 30.037 | 75.914 | 2.53 |
| Ketone thiophene proportioning 9:1 | 33.252 | 69.603 | 2.09 |
As can be seen from the test results, triazolone to the virulence of several disease fungus of ear rot higher than the gloomy copper of thiophene; Triazolone and the composite SR value to new long-radius elbow spore preventive effect of thiophene gloomy copper 1:1 the highest, reach 6.60; Triazolone and the SR value of thiophene gloomy copper 1:9 proportioning to fusarium prolifertum minimum, be 1.94; The SR value of triazolone and each compound proportion of the gloomy copper of thiophene is all greater than 1.5, all shows synergistic effect.
In this test example, triazolone and the gloomy copper of thiophene adopt other ratio, as 10:1,8:1,5:1,2:1,1:2,1:5,1:8 or 1:10 carry out the test as test example 1, the inhibitory action of Recompounded pesticide to Rice Panicle maize ear rot bacterium of last above-mentioned different ratio amount is all stronger, coefficient of synergism, all higher than 1.5, shows strong synergistic effect.
Equally, triazolone and each compound proportion of the gloomy copper of thiophene all show similar synergistic effect in the Toxicity Determination of the disease funguses such as rice blast fungus, fusarium moniliforme and Rhizoctonia solani Kuhn.
Test example 2: triazolone and the composite Toxicity Determination to paddy bacterial brown patch germ of the gloomy copper of thiophene
2.1 medicament preparations
With 1.1 of test example 1.
2.2 test strain
Paddy bacterial brown spot germ: pseudomonas syringae cloves pvs oryzae and oryzicola (
pseudomonas syringaepv.
syringae).
2.3 test method
Triazolone and the composite Toxicity Determination to paddy bacterial Pathogenic Bacteria Causing Brown Blotch Disease of the gloomy copper of thiophene adopt the method for plate culture count to carry out.
Test and on suitable test tube slant medium, be cultured to lawn generation with pathogenetic bacteria, get cultured bacterium inclined-plane, add a certain amount of sterile saline or other suitable dilution.Under aseptic technique, gently scrape lawn surface with transfer needle, obtain bacterial suspension.Be placed in constant temperature oscillator and mix 20 minutes with the rotating speed of 120 revs/min.With stroke-physiological saline solution or other suitable dilution, bacterial suspension being diluted to blank clump count can clearly separate, and is no less than 200.With pipette or pipettor from low concentration to high concentration, draw liquid 0.5mL successively and add respectively in small test tube, then draw the bacterial suspension 1.5mL prepared, make liquid and bacterial suspension mixed in equal amounts even.Add 100ml liquid nutrient medium, cultivate 5 ~ 6 hours, get 2ml culture fluid add melt in advance after be cooled to the quantitative sterilising medium (being generally 60ml) of 46 DEG C, add in aseptic conical flask, fully shake up.Before medium does not solidify, pour plate, then equivalent pours more than 3 diameters into is in the culture dish of 9cm.After solidifying, be positioned in microbiological incubator, under 28 DEG C of conditions, be cultured to bacterium colony on blank clear and legible.Each plate count, can detect by an unaided eye, use lens examination if desired, in case omit.
According to colony counting result, calculate each concentration for the treatment of by formula (5) and form inhibiting rate to the bacterium colony for examination target bacterium, unit is percentage (%), and result of calculation retains 2 significant digits.
…………………………………………………………（5）
In formula:
I-bacterium colony forms inhibiting rate;
-blank bacterium colony forms number;
-chemicals treatment bacterium colony forms number.
The probability value forming inhibiting rate according to the bacterium colony of each drug concentration logarithm value and correspondence does regression analysis, calculates the value of each medicament.
2.4 composite effect assessments
On the indoor virulence to paddy bacterial brown spot, the composite effect assessment of two kinds of each proportionings of medicament, as described in 1.4 of test example 1.
2.5 result of the test
Triazolone and the gloomy copper single dose of thiophene and built agent are to the Toxicity Determination result of paddy bacterial brown spot, as shown in table 5:
Table 5 triazolone and thiophene gloomy copper single dose and each proportioning are to the indoor virulence of paddy rice brown patch germ
| Reagent agent | Actual EC 50（ppm） | Theoretical EC 50(ppm) | Coefficient of synergism |
| Triazolone | 2157.23 | / | / |
| The gloomy copper of thiophene | 11.36 | / | / |
| Ketone thiophene proportioning 1:9 | 6.204 | 12.615 | 2.03 |
| Ketone thiophene proportioning 1:4 | 5.398 | 14.181 | 2.63 |
| Ketone thiophene proportioning 3:7 | 4.996 | 16.192 | 3.24 |
| Ketone thiophene proportioning 2:3 | 4.203 | 18.867 | 4.49 |
| Ketone thiophene proportioning 1:1 | 3.049 | 22.601 | 7.41 |
| Ketone thiophene proportioning 3:2 | 3.860 | 28.177 | 7.30 |
| Ketone thiophene proportioning 7:3 | 6.148 | 37.407 | 6.08 |
| Ketone thiophene proportioning 4:1 | 8.715 | 55.628 | 6.38 |
| Ketone thiophene proportioning 9:1 | 29.262 | 108.460 | 3.71 |
As can be seen from this result of the test, triazolone is strong to the inhibitory action of paddy bacterial brown spot, and its single dose EC50 is up to 2157.23; And the gloomy copper of thiophene is better to paddy bacterial brown spot effect, its single dose EC
50be only 11.36.In the inhibitory action of paddy bacterial Pathogenic Bacteria Causing Brown Blotch Disease, the SR value of each compound proportion, the highest is triazolone: thiophene gloomy copper 1:1 proportioning, reaches 7.41, and minimum is triazolone: thiophene gloomy copper 1:9 proportioning, also reaches 2.03.Triazolone and each compound proportion of the gloomy copper of thiophene have all exceeded 1.5, show synergistic effect.
In this test example, triazolone and the gloomy copper of thiophene adopt other ratio, as 10:1,8:1,5:1,2:1,1:2,1:5,1:8 or 1:10 carry out the test as test example 1, the Recompounded pesticide of last above-mentioned different ratio amount is all stronger to the inhibitory action of paddy bacterial brown patch germ, coefficient of synergism, all higher than 1.5, shows strong synergistic effect.
Equally, triazolone and each compound proportion of the gloomy copper of thiophene are to the synergistic effect all showing similar test example 2 in the Toxicity Determination of the pathogenetic bacterias such as water Xanthomonas oryzae pv.oryzae, X. c. pv. oryzicola bacterium and Burkholderia glumae.
Test example 3: triazolone and the composite field control effectiveness test to rice green smut and Rice Panicle maize ear rot of the gloomy copper of thiophene
In order to verify triazolone and the composite field control effect to rice green smut and Rice Panicle maize ear rot of the gloomy copper of thiophene, in November ,-2013 in July, 2013, base tested by the rice in China of Fuyang, Hangzhou, carry out triazolone and the composite field control effectiveness test to rice green smut and Rice Panicle maize ear rot of the gloomy copper of thiophene, achieve significant control efficiency.
3.1 test kinds
Late-maturing three is excellent No. 12 of Indica-Japonica Hybrid Rice river in Zhejiang Province.
3.2 controlling object
Rice green smut and Rice Panicle maize ear rot.
3.3 chemicals treatment arrange (active ingredient is all 30 grams/acre)
(1) 25% triazolone and composite 120 grams/acre of thiophene gloomy copper 1:4;
(2) 25% triazolones and composite 120 grams/acre of thiophene gloomy copper 3:2;
(3) 25% triazolones and composite 120 grams/acre of thiophene gloomy copper 7:3;
(4) 25% trazodone wettable powders 120 grams/acre (positive medicament contrast);
(5) 20% thiophene gloomy copper suspending agent 150 grams/acre (positive medicament contrast);
(6) clear water contrast;
Each process repetition 4 times, arranges 24 each communities altogether, 30 square metres, every community.
3.4 medicament usings method
Be watered 45,000 grams/acre, paddy rice cut the last week, spray.
3.5 investigation and investigation methods
After medicine 35 days, 5 points were selected in every community, often some investigation 200 fringes, by the incidence of corresponding grade scale investigation rice green smut and Rice Panicle maize ear rot.
The grade scale of rice green smut is as shown in the table
| Sick level | 0 | 1 | 3 | 5 | 7 | 9 |
| Infected seed number/fringe | 0 | 1 | 2-4 | 5-8 | 9-14 | ≥15 |
| Fringe heavily loses % | 0 | 0.1-5.0 | 5.1-10.0 | 10.1-20.0 | 20.1-50.0 | ≥50.1 |
The grade scale of Rice Panicle maize ear rot is as shown in the table:
Computing formula and statistical analysis:
Deng Kenshi new multipole difference (DMRT) method is adopted to carry out statistical analysis to test data and compare.
3.6 result of the test
Triazolone and the gloomy copper of thiophene is composite prevents and treats test of pesticide effectiveness result to rice green smut and Rice Panicle maize ear rot, as shown in table 6:
Table 6 triazolone and the gloomy copper of thiophene composite to rice green smut and Rice Panicle maize ear rot field efficacy result
From to the preventive effect of rice green smut, when effective ingredient consumption is identical, three compound drug process, process 1(25% triazolone and thiophene gloomy copper 1:4 composite), process 2(25% triazolone and thiophene gloomy copper 3:2 composite) and process 3(25% triazolone and thiophene gloomy copper 7:3 composite) preventive effect be respectively 80.40%, 86.62% and 83.57%, be significantly higher than process 4(25% trazodone wettable powder) and the gloomy copper suspending agent of process 5(20% thiophene), triazolone and the composite control efficiency excellence of the gloomy copper of thiophene on the field efficacy of rice green smut are described, show very strong synergistic effect simultaneously.
The preventive effect to Rice Panicle maize ear rot, when effective ingredient consumption is identical, three compound drug process, process 1(25% triazolone and thiophene gloomy copper 1:4 composite), process 2(25% triazolone and thiophene gloomy copper 3:2 composite) and process 3(25% triazolone and thiophene gloomy copper 7:3 composite) preventive effect can reach 85.02% respectively, 85.24% and 87.87%, be better than process 4(25% trazodone wettable powder significantly) and the gloomy copper suspending agent of process 5(20% thiophene), triazolone and the composite control efficiency excellence of the gloomy copper of thiophene on the field efficacy of Rice Panicle maize ear rot are described, show very strong synergistic effect simultaneously.
On the whole, in the microbial disease of this purity of rice green smut, triazolone performance better, can reach 73.78%; The performance of thiophene gloomy copper is poor, is only 62.69%.And to the disease aspect that this multiple fungus and bacterium Combined Infection of Rice Panicle maize ear rot causes, the gloomy copper of thiophene is slightly better than triazolone, can 79.48% be reached.And triazolone and the gloomy copper of thiophene composite after, under effective ingredient consumption same case, preventive effect is all significantly better than the contrast of two single doses, and triazolone and the gloomy copper of the thiophene composite expansion controlling object that improves are described, raising control efficiency.
In this test example, triazolone and the gloomy copper of thiophene adopt other ratio, as 10:1,9:1,8:1,5:1,4:1,2:1,2:3,1:2,1:5,1:8 or 1:10 carry out the test as test example 3, the Recompounded pesticide of last above-mentioned different ratio amount all can reach more than 82% to rice green smut and Rice Panicle maize ear rot control efficiency, shows strong synergistic effect.
Test example 4: triazolone and the composite field control effectiveness test to rice leaf sheath rot and bacterial blight of rice of the gloomy copper of thiophene
In order to verify triazolone and the composite control efficiency to rice leaf sheath rot and bacterial blight of rice of the gloomy copper of thiophene, in July, 2013 to November, in field trial base, China Paddy Rice Inst Fuyang, triazolone and the composite field control effectiveness test to rice leaf sheath rot and bacterial blight of rice of the gloomy copper of thiophene are carried out.
4.1 test kinds
Rich two excellent fragrant No. one.
4.2 controlling object
Rice leaf sheath rot and bacterial blight of rice.
4.3 chemicals treatment arrange (active ingredient is all 30 grams/acre)
(1) 25% triazolone and composite 120 grams/acre of thiophene gloomy copper 2:3;
(2) 25% triazolones and composite 120 grams/acre of thiophene gloomy copper 3:2;
(3) 25% triazolones and composite 120 grams/acre of thiophene gloomy copper 4:1;
(4) 25% trazodone wettable powders 120 grams/acre (positive medicament contrast);
(5) 20% thiophene gloomy copper suspending agent 150 grams/acre (positive medicament contrast); ;
(6) clear water contrast;
Each process repetition 4 times, arranges 24 each communities altogether, 30 square metres, every community.
4.4 medicament usings method
Be watered 45,000 grams/acre, paddy rice cut the last week, spray.
4.5 investigation and investigation methods
After dispenser 15 days, by corresponding grade scale investigation bacterial leaf-blight, a situation arises; After dispenser 20 days, a situation arises for investigation rice leaf sheath rot.
The field grade scale of bacterial blight of rice is as shown in the table:
| sick level | resistant reaction | evaluation of resistance |
| 0 | scab length is less than inoculation blade residue length 5.0%; Or lesion area is less than 5.0%. | high resistance (HR) |
| 1 | scab length accounts for inoculation blade residue length 5.1% ~ 12.0%; Or lesion area accounts for leaf area 5.1% ~ 12.0%. | anti-(R) |
| 3 | scab length accounts for inoculation blade residue length 12.1% ~ 25.0%; Or lesion area accounts for leaf area 12.1% ~ 25.0%. | in anti-(MR) |
| 5 | scab length accounts for inoculation blade residue length 25%.1 ~ 50.0%; Or lesion area accounts for leaf area 25.1% ~ 50.0%. | middle sense (MS) |
| 7 | scab length accounts for inoculation blade residue length 50.1% ~ 75.0%; Or lesion area accounts for leaf area 50.1% ~ 75.0%. | sense (S) |
| 9 | scab length is greater than inoculation blade residue length 75.1%; Or lesion area is greater than leaf area 75.1%. | high sense (HS) |
Bacterial blight of rice preventive effect computing formula:
The preventive effect of rice leaf sheath rot calculates according to the fringe incidence of disease:
Deng Kenshi new multipole difference (DMRT) method is adopted to carry out statistical analysis to test data and compare.
4.6 result of the tests:
The control of triazolone and the gloomy copper of thiophene composite water prevention sheath rot of rice plant and bacterial blight of rice achieves significant control efficiency, and result is as shown in table 7:
Table 7 triazolone and the composite field efficacy to rice leaf sheath rot and bacterial blight of rice of the gloomy copper of thiophene
When active ingredient dosage is identical, 3 compound drug process, process 1(25% triazolone and thiophene gloomy copper 2:3 composite), process 2(25% triazolone and thiophene gloomy copper 3:2 composite) and process 3(25% triazolone and thiophene gloomy copper 4:1 composite) 86.28%, 86.88% and 87.93% is reached respectively to the preventive effect of bacterial blight of rice, be significantly higher than positive control medicament process 4(25% trazodone wettable powder) and process 5(20% thiophene gloomy copper suspending agent).Therefore, triazolone and the gloomy copper of thiophene is composite can efficiently prevention and control bacterial blight of rice, and both are composite is showing synergistic effect in the control efficiency of bacterial leaf-blight.
When active ingredient dosage is identical, 3 compound drug process, process 1(25% triazolone and thiophene gloomy copper 2:3 composite), process 2(25% triazolone and thiophene gloomy copper 3:2 composite) and process 3(25% triazolone and thiophene gloomy copper 4:1 composite) all more than 80% is reached to the preventive effect of rice leaf sheath rot, preventive effect is higher than contrast chemicals treatment 4(25% trazodone wettable powder), be significantly higher than the gloomy copper suspending agent of process 5(20% thiophene), show good control efficiency.Triazolone and the gloomy copper of thiophene also show synergistic effect in the preventive effect to rice leaf sheath rot.
Triazolone is not enough to bacterial blight of rice preventive effect, and the gloomy copper of thiophene is to the poor effect of rice leaf sheath rot.And have overlap due to bacterial blight of rice and rice leaf sheath rot proper control time, triazolone and the gloomy copper of thiophene composite after not only add prevention and treatment range, preventive effect also shows obvious synergistic effect.
In this test example, triazolone and the gloomy copper of thiophene adopt other ratio, as 10:1,9:1,8:1,5:1,2:1,2:3,1:2,1:4,1:5,1:8 or 1:10 carry out the test as test example 4, the Recompounded pesticide of last above-mentioned different ratio amount all can reach more than 85% to bacterial blight of rice and rice leaf sheath rot control efficiency, shows strong synergistic effect.
Claims (7)
1. contain a Recompounded pesticide for triazolone and the gloomy copper of thiophene, it is characterized in that, containing active ingredient triazolone and the gloomy copper of thiophene, the weight ratio of described triazolone and the gloomy copper of thiophene is 1:9 ~ 9:1.
2. a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene as claimed in claim 1, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:4 ~ 1:1.
3. a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene as claimed in claim 1, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:1 ~ 9:1.
4. a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene as claimed in claim 1, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 1:4 ~ 2:3.
5. a kind of Recompounded pesticide containing triazolone and the gloomy copper of thiophene as claimed in claim 1, is characterized in that the weight ratio of described triazolone and the gloomy copper of thiophene is 3:2 ~ 4:1.
6. as in claim 1-5, any one contains the application of Recompounded pesticide in water prevention rice seed-borne disease of triazolone and the gloomy copper of thiophene.
7. apply as claimed in claim 6, it is characterized in that described paddy rice seed-borne disease is Rice Panicle maize ear rot, rice blast, bakanae disease of rice, rice sheath blight disease, paddy bacterial brown spot, water Xanthomonas oryzae pv.oryzae, X. c. pv. oryzicola bacterium, Burkholderia glumae, rice green smut and rice leaf sheath rot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410054435.4A CN103798251B (en) | 2014-02-18 | 2014-02-18 | A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410054435.4A CN103798251B (en) | 2014-02-18 | 2014-02-18 | A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103798251A CN103798251A (en) | 2014-05-21 |
| CN103798251B true CN103798251B (en) | 2015-10-28 |
Family
ID=50696026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410054435.4A Expired - Fee Related CN103798251B (en) | 2014-02-18 | 2014-02-18 | A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103798251B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633890A (en) * | 2018-04-25 | 2018-10-12 | 浙江东风化工有限公司 | A kind of bactericidal composition containing metconazole Yu the gloomy copper of thiophene |
| CN110881476A (en) * | 2018-09-07 | 2020-03-17 | 刘力 | High-efficiency broad-spectrum low-toxicity environment-friendly agricultural new compound and composition thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85101435A (en) * | 1985-04-01 | 1986-07-09 | 四川省化学工业研究所 | Novel cpd N, N '-methylene-two (2 amino-5 sulfydryl-1,3,4 thiadiazoles) preparation method and contain the control vegetative bacteria of this compound, the disease sterilant |
| CN1311996A (en) * | 2000-01-28 | 2001-09-12 | 山东大成农药股份有限公司 | Preparation of triazolone-containing mixture with bactericidal activity |
| CN102742571A (en) * | 2012-07-24 | 2012-10-24 | 西安近代农药科技股份有限公司 | Cuppric nonyl phenolsulfonate-containing compound water-based pesticide and preparation method as well as application thereof |
| WO2013186325A1 (en) * | 2012-06-15 | 2013-12-19 | Stichting I-F Product Collaboration | Synergistic compositions for the protection of agrarian crops and the use thereof |
-
2014
- 2014-02-18 CN CN201410054435.4A patent/CN103798251B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85101435A (en) * | 1985-04-01 | 1986-07-09 | 四川省化学工业研究所 | Novel cpd N, N '-methylene-two (2 amino-5 sulfydryl-1,3,4 thiadiazoles) preparation method and contain the control vegetative bacteria of this compound, the disease sterilant |
| CN1311996A (en) * | 2000-01-28 | 2001-09-12 | 山东大成农药股份有限公司 | Preparation of triazolone-containing mixture with bactericidal activity |
| WO2013186325A1 (en) * | 2012-06-15 | 2013-12-19 | Stichting I-F Product Collaboration | Synergistic compositions for the protection of agrarian crops and the use thereof |
| CN102742571A (en) * | 2012-07-24 | 2012-10-24 | 西安近代农药科技股份有限公司 | Cuppric nonyl phenolsulfonate-containing compound water-based pesticide and preparation method as well as application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 新颖杀菌剂——噻森铜;张纯标 等;《世界农药》;20070430;第29卷(第2期);第53页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103798251A (en) | 2014-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103798251B (en) | A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene | |
| CN108294014A (en) | A kind of seed coat agent and the preparation method and application thereof of prevention root rot | |
| CN104094942B (en) | A kind of 2-cyano-3-amino-3-phenylancryic acetate-containing and hymexazol composition pesticide | |
| CN104522035B (en) | A kind of containing methylsulfonyl bacterium azoles and the complex composition of fenaminosulf and preparation | |
| CN107212011B (en) | A kind of bactericidal composition for preventing and treating crop disease | |
| CN103814922B (en) | A kind of bactericidal composition containing carbendazim and copper rosinate | |
| CN103798291B (en) | A kind of Recompounded pesticide containing triazolone and Cupravit | |
| CN108770858A (en) | Pyraclostrobin is being prepared with probenazole for the purposes in preventing the fungicide of the plant disease caused by Rhizoctonia cerealis | |
| CN103798262B (en) | A kind of bactericidal composition containing thiophanate-methyl and copper rosinate | |
| CN104094939B (en) | A kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole | |
| CN103798273B (en) | The Recompounded pesticide of a kind of composite containing streptomycin sulphate and application thereof | |
| CN103814949B (en) | A kind of Recompounded pesticide containing triazolone and cuprous oxide | |
| CN107242242A (en) | Bactericidal composition comprising folpet and prothioconazoles | |
| CN104488930B (en) | A kind of complex composition and preparation of the azoles of bacterium containing methylsulfonyl and Zhongshengmycin | |
| CN110800754B (en) | Compound preparation for preventing and treating peach bacterial perforation disease and application | |
| CN103798270B (en) | A kind of Recompounded pesticide and application thereof containing the gloomy copper of thiophene | |
| CN104938514B (en) | Prevent and treat the phytochemical synergistic composition of rice blast | |
| CN104920358B (en) | Natural product combination for preventing and controlling rice blast | |
| CN104920359B (en) | Plant essential oil for preventing and controlling rice blast efficiently | |
| CN105076152B (en) | A kind of microbicide compositions and its application | |
| CN108432787A (en) | A kind of bactericidal composition containing thiophanate methyl Yu the gloomy copper of thiophene | |
| CN107960416A (en) | A kind of bactericidal composition and its application containing dimethomorph | |
| CN108849946A (en) | A kind of bactericidal composition and application thereof containing folpet and epoxiconazole | |
| CN107969438B (en) | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof | |
| CN105941482A (en) | Compound pesticide containing Zhongshengmycin and Saisentong and application of compound pesticide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151028 Termination date: 20160218 |