CN107969438B - Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof - Google Patents
Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof Download PDFInfo
- Publication number
- CN107969438B CN107969438B CN201711376304.8A CN201711376304A CN107969438B CN 107969438 B CN107969438 B CN 107969438B CN 201711376304 A CN201711376304 A CN 201711376304A CN 107969438 B CN107969438 B CN 107969438B
- Authority
- CN
- China
- Prior art keywords
- trichoderma harzianum
- wettable powder
- powder containing
- ammine
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention discloses wettable powder containing trichoderma harzianum T-22 and cuaminosulfate as well as a preparation method and application thereof, relates to the field of agriculture, and particularly relates to wettable powder for preventing and treating soil-borne diseases of crops. The wettable powder containing Trichoderma harzianum T-22 and copper ammine is good in prevention and treatment effect, wide in prevention and treatment spectrum, safe and pollution-free, and the preparation method and the application thereof are provided. The wettable powder containing trichoderma harzianum T-22 and cupric ammine comprises trichoderma harzianum T-22 and cupric ammine. According to the product, the trichoderma harzianum T-22 and the cuaminosulfate are compounded to be used for preventing and treating the soil-borne diseases of crops, the root soil-borne diseases can be effectively controlled by one-time use, the prevention and treatment effect is improved, the prevention and treatment spectrum is increased, and the good prevention and treatment effect is achieved on the soil-borne diseases mixed by various pathogenic bacteria.
Description
Technical Field
The invention relates to the field of agriculture, in particular to wettable powder for preventing and treating soil-borne diseases of crops.
Background
Trichoderma harzianum T-22 is a preventive microbial bactericide, can be used in the organic crop planting process, has good control effect on root diseases, and the duration of the drug effect can reach three months, but Trichoderma harzianum T-22 as a microbial preparation has slow effect, good preventive and use effects, and takes effect slowly after onset of disease. The copper preparation can prevent and treat fungal diseases and bacterial diseases, has the functions of protection and prevention, has certain treatment and eradication functions, adopts the complex of copper glycinate to effectively reduce the problem that the common copper preparation is easy to cause phytotoxicity, and can be also used in organic planting production. The two are compounded, so that the problem of poor prevention and treatment effect of singly using one preparation in actual agricultural production can be solved.
Disclosure of Invention
The invention aims to solve the technical problem of providing the wettable powder containing the trichoderma harzianum T-22 and the cuaminosulfate, which has good prevention and treatment effect, wide prevention and treatment spectrum, safety and no pollution, and the preparation method and the application thereof.
The invention relates to wettable powder containing trichoderma harzianum T-22 and cupric ammine, which comprises trichoderma harzianum T-22 and cupric ammine.
Preferably, the content of Trichoderma harzianum T-22 in the wettable powder is 1X 107～1×109CFU/g; the content of the copper ammine complexes is 10-25 wt%.
Preferably, the wettable powder further comprises a wetting agent, a dispersing agent and a carrier.
Preferably, the content of the wetting agent in the wettable powder is 1-5 wt%; the content of the dispersing agent is 1-5 wt%; the carrier is present to make up 100 wt%.
Preferably, the wetting agent comprises one or more of sodium lignosulfonate, 2-naphthalene sulfonic acid formaldehyde polymer sodium salt (NNO), fatty alcohol polyoxyethylene ether (JFC), sodium lauryl sulfate.
Preferably, the dispersant comprises one or more of sodium salt of 2-naphthalenesulfonic acid-formaldehyde polymer (NNO), polyethylene glycol, fatty alcohol-polyethylene glycol ester, sodium cellulose, and sodium tripolyphosphate.
Preferably, the carrier comprises one or more of diatomite, bentonite, kaolin, talcum powder and white carbon black.
The invention also relates to a preparation method of the wettable powder containing Trichoderma harzianum T-22 and copper ammine, which comprises the following steps: the raw materials except the Trichoderma harzianum T-22 are mixed and crushed, then spore powder of the Trichoderma harzianum T-22 is added, and the mixture is uniformly mixed again, so that the wettable powder containing the Trichoderma harzianum T-22 and the cupric ammine is prepared.
The raw materials comprise Trichoderma harzianum T-22 and copper ammine.
Preferably, the content of Trichoderma harzianum T-22 in the raw material is 1X 107～1×109CFU/g; the content of the copper ammine complexes is 10-25 wt%.
Preferably, the raw materials further comprise a wetting agent, a dispersing agent and a carrier.
The invention also relates to application of wettable powder containing Trichoderma harzianum T-22 and cupric ammine, wherein the wettable powder containing Trichoderma harzianum T-22 and cupric ammine is the wettable powder in claim 1, and is used for preventing and treating soil-borne diseases of crops.
Preferably, the crops comprise vegetables, flowers, Chinese medicinal materials, seedlings and fruit trees.
Compared with the prior art, the invention discloses wettable powder containing Trichoderma harzianum T-22 and copper ammine, and a preparation method and application thereof, wherein the wettable powder comprises the following components:
1. the wettable powder containing the trichoderma harzianum T-22 and the cuaminosulfate is a compound preparation, has an increased prevention and treatment spectrum on diseases, has a wider prevention and treatment range and better effect than those of two separate preparations, and can effectively prevent and treat soil-borne diseases caused by various pathogenic bacteria; and the two compounded medicaments both accord with the organic planting standard, and the compounded preparation can be used in organic planting production as well, thereby providing a high-quality medicament for the organic planting production.
2. According to the product, the trichoderma harzianum T-22 and the cuaminosulfate are compounded to be used for preventing and treating the soil-borne diseases of crops, the root soil-borne diseases can be effectively controlled by one-time use, the prevention and treatment effect is improved, the prevention and treatment spectrum is increased, and the good prevention and treatment effect is achieved on the soil-borne diseases mixed by various pathogenic bacteria.
Detailed Description
The wettable powder of the present invention containing Trichoderma harzianum T-22 and cupric ammine, and its preparation method and application are further illustrated by the following examples and confirmatory tests.
Examples
The wettable powder containing trichoderma harzianum T-22 and copper ammine of the embodiment is prepared by the following steps: mixing and crushing the raw materials except the Trichoderma harzianum T-22, adding spore powder of the Trichoderma harzianum T-22, and uniformly mixing again to obtain the wettable powder containing the Trichoderma harzianum T-22 and the cupric ammine; the starting materials are shown in table 1.
TABLE 1 wettable powder raw material containing Trichoderma harzianum T-22 and cupric ammine of this example
Note: table is not filled.
Verification test
The prevention and control effect of the product is verified by adopting a method combining indoor toxicity measurement and field test.
Test subjects: watermelon fusarium wilt (fusarium oxysporum watermelon specialization type), purchased by the chinese agricultural microbial strain preservation management center.
Detecting the indoor toxicity of the ammoniacal cupric complexes on the trichoderma harzianum T-22 and the fusarium oxysporum by adopting a hypha growth rate method, and detecting the indoor toxicity of the trichoderma harzianum T-22 on the fusarium oxysporum; a hypha growth rate method is adopted to measure the indoor toxicity of the mixture of the cuprammonium chloride and the Trichoderma harzianum T-22 in different proportions to Fusarium oxysporum.
1. Method for measuring indoor toxicity of ammoniacal copper complex on trichoderma harzianum T-22 and fusarium oxysporum by hypha growth rate method
Hypha growth rate method test method:
preparation of a poison-containing culture medium: heating and melting the sterilized PDA culture medium, adding a certain amount of medicament solution, uniformly mixing to make the concentration reach the concentration required to be tested, then pouring into a culture dish, adding 20mL of medicament solution with the diameter of 75mm, and adding 25mL of medicament solution with the diameter of 90 mm.
The determination method comprises the following steps: the test is carried out according to the agricultural industry standard (NY/T1156.2-2006) of the people's republic of China by adopting a hypha growth rate method. And (3) cutting the cultured Trichoderma harzianum T-22 from the edge of a bacterial colony by using a sterilization puncher with the diameter of 6mm under the aseptic operation condition, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, and culturing in an incubator at 25 ℃. After a certain time, the colony diameter was measured by the cross method. Each treatment concentration was set to 3 replicates.
And (4) investigating a result: and (3) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the hyphae in a blank control culture dish, measuring the diameters of the colonies by using calipers, vertically measuring the diameter of each colony by using a cross method once, and then repeatedly averaging several colonies.
And (3) calculating an inhibition rate: based on the results of the investigation, the inhibition ratios were calculated by the formulas (1) and (2) in percentage
D＝D1-D2………………………………………………………………(1)
In the formula:
d, colony growing diameter;
D1-colony diameter;
D2-cake diameter.
In the formula:
i-inhibition rate of hypha growth;
D0-blank control colony growth diameter;
Dt-processing the colony growing diameter.
EC50The calculation of (2): the pathogen inhibition rate is converted into several values, which are used as y values as ordinate and logarithmic values of concentrationMaking virulence regression curve with the abscissa and the correlation coefficient R2Is greater than 0.9. When the inhibition rate is 50%, the value y is 5.0, the value x is obtained, and the value x is exponentiated and converted into a concentration value, namely EC50The value is obtained.
TABLE 2 determination of inhibitory Effect of cupric ammine on Trichoderma harzianum T-22 by hypha growth Rate method
| Bactericide | Regression equation of virulence | EC50(μg/mL) | Coefficient of correlation (R)2) |
| Copper ammine complexes | y＝0.773x+1.906 | 10059.8 | 0.988 |
The inhibition effect of the copper ammine on the trichoderma harzianum T-22 is very weak, and the copper ammine compounded by the copper ammine and the copper ammine basically cannot inhibit the trichoderma harzianum T-22.
TABLE 3 determination of inhibition of Amazon on Fusarium oxysporum by hypha growth rate method
| Bactericide | Regression equation of virulence | EC50(μg/mL) | Coefficient of correlation (R)2) |
| Copper ammine complexes | y＝2.41x-0.949 | 294.1 | 0.978 |
2. Indoor toxicity of Trichoderma harzianum T-22 to Fusarium oxysporum
Taking Trichoderma harzianum spore powder with known content, preparing into powder with concentration of 1 × 10 per mL by distilled water9、l×108、l×l07、1×106And 1X 105A bacterial suspension of individual spores. 2mL of the Trichoderma harzianum suspension at each concentration was added to 18mL of sterilized PDA at a temperature of about 40 ℃ to prepare a PDA plate containing the microorganism. The center of the plate was inoculated with a cake of activated Fusarium oxysporum (diameter 6mm) after 3 days at 25 ℃. The treatment of adding sterilized water to PDA plates and inoculating Fusarium oxysporum was used as a blank control, and the treatment was repeated 4 times per concentration, and the test was repeated 3 times. Inverting the PDA plate into a constant temperature incubator at 25 ℃ for 3d, measuring the diameter of fusarium oxysporum colonies by a cross method, calculating the inhibition rate (%), and calculating EC50。
TABLE 4 inhibition of Fusarium oxysporum by Trichoderma harzianum T-22
| Bactericide | Regression equation of virulence | EC50(one/mL) | Coefficient of correlation (R)2) |
| Trichoderma harzianum T-22 | y＝0.608x+1.702 | 2.66×105 | 0.965 |
3. Method for determining inhibition effect of combination of trichoderma harzianum and cuprammonium on fusarium oxysporum by hypha growth rate method
The cupric ammine and the trichoderma harzianum T-22 are mixed in different proportions, and the inhibition effect of the mixed medicament on the fusarium oxysporum is measured according to the conventional dilution multiple of 1000 times.
The concentrations of the cuaminosulfate after dilution by 1000 times are respectively 100 mug/mL, 150 mug/mL, 200 mug/mL and 250 mug/mL.
Trichoderma harzianum T-221X 107CFU/g、2×107CFU/g、1×108CFU/g、3×108CFU/g, 1X 10 concentration after dilution4CFU/g、2×104CFU/g、1×105CFU/g、3×105CFU/g。
TABLE 4 determination of inhibition of Fusarium oxysporum by combination of cupric ammine and Trichoderma harzianum T-22 by hypha growth rate method
4. 25% cuaminosulfate 3 hundred million CFU/gram Trichoderma harzianum wettable powder for treating watermelon fusarium wilt in field
The test method comprises the following steps: 1 mu of watermelon is planted. The medicinal liquid is diluted 1000 times and irrigated with roots, and the watermelon is irrigated with roots at the early stage of morbidity after transplantation, and the dosage of each seedling is 200mL, and the application is carried out once. During the test period, no other pesticide for preventing and treating other diseases and pests is used, 25% cuaminosulfate is selected to be filled alone as a control group 1, 3 hundred million CFU/gram Trichoderma harzianum wettable powder is selected to be filled alone as a control group 2, and clear water is selected as a blank control.
Investigation time: and (4) investigating the number of diseased seedlings of all the cells when the blank control cells have obvious diseases, and investigating once in 10 days and 20 days after root irrigation, wherein the investigation is carried out twice in total.
Sampling is carried out at five random points in each cell, about 30 plants are investigated at each point, the roots of watermelon seedlings are investigated, and the number of plants suffering from blight and the total number of plants are investigated.
Test results
The control effect of the test group is 87.24%, the control effect of the control group 1 is 56.23%, and the control effect of the control group 2 is 60.21, and experiments prove that the combination of the two can obviously improve the control effect on the watermelon fusarium wilt.
The using method of the invention is as follows:
mixing soil: 400 g of mixed soil (matrix) of 400 g of a compound preparation of trichoderma harzianum T-22 and copper ammine;
spraying the seedbed: after seedling emergence, the preparation is diluted and sprayed for use, and the concentration of the preparation is 4-6 g/m2；
Irrigating roots of single plants: the preparation is diluted 1000 times, and the roots are irrigated by single plants, and each plant is irrigated by 100-200 ml.
The product of the invention is a compound preparation of trichoderma harzianum T22 and cuaminosulfate, can be used for economic crops such as vegetables, flowers, traditional Chinese medicinal materials, nursery stocks, fruit trees and the like, and is used for preventing and treating soil-borne diseases of the crops.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that these are by way of example only, and that the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention.
Claims (5)
1. A wettable powder containing Trichoderma harzianum T-22 and copper ammine, which is characterized in that: the wettable powder comprises trichoderma harzianum T-22 and copper ammine, and the content of the trichoderma harzianum T-22 in the wettable powder is 1 multiplied by 107～1×108CFU/g; the content of the complex ammoniacal copper is 20-25 wt%; the wettable powder also comprises a wetting agent, a dispersing agent and a carrier.
2. The wettable powder containing trichoderma harzianum T-22 and cupric ammine according to claim 1, characterized in that: the preparation method comprises the following steps: mixing the raw materials except the Trichoderma harzianum T-22, crushing, adding spore powder of the Trichoderma harzianum T-22, and uniformly mixing again to obtain wettable powder containing the Trichoderma harzianum T-22 and copper ammine;
the raw materials comprise Trichoderma harzianum T-22 and copper ammine.
3. The wettable powder containing trichoderma harzianum T-22 and cupric ammine according to claim 2, characterized in that: the raw materials also comprise a wetting agent, a dispersing agent and a carrier.
4. The application of wettable powder containing Trichoderma harzianum T-22 and cupric ammine is characterized in that: the wettable powder containing trichoderma harzianum T-22 and cuaminosulfate is the wettable powder in claim 1, and is used for controlling soil-borne diseases of crops.
5. The use of a wettable powder containing trichoderma harzianum T-22 and cupric ammine according to claim 4, characterized in that: the crops comprise vegetables, flowers, traditional Chinese medicinal materials and fruit trees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711376304.8A CN107969438B (en) | 2017-12-19 | 2017-12-19 | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711376304.8A CN107969438B (en) | 2017-12-19 | 2017-12-19 | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107969438A CN107969438A (en) | 2018-05-01 |
| CN107969438B true CN107969438B (en) | 2021-04-06 |
Family
ID=62006744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711376304.8A Active CN107969438B (en) | 2017-12-19 | 2017-12-19 | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107969438B (en) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1041864A (en) * | 1988-10-15 | 1990-05-09 | 李志刚 | Insecticide |
| US20040261578A1 (en) * | 2003-04-04 | 2004-12-30 | Harman Gary E | Stable self-organizing plant-organism systems for remediating polluted soils and waters |
| WO2015011615A1 (en) * | 2013-07-22 | 2015-01-29 | Basf Corporation | Mixtures comprising a trichoderma strain and a pesticide |
| CN104322558B (en) * | 2014-09-30 | 2017-04-26 | 四川利尔作物科学有限公司 | Sterilization composition and applications thereof |
| CN105028487A (en) * | 2015-07-14 | 2015-11-11 | 河南科技大学 | Bactericide for preventing and treating wheat scab and preparation method for bactericide |
| ITUB20153821A1 (en) * | 2015-09-23 | 2017-03-23 | Agrocube S A | BIOSTIMULATING COMPOSITION BASED ON TANNINS AND / OR THEIR DERIVATIVES, PR THE TREATMENT OF CULTIVATED AND / OR VEGETABLE VEGETABLES |
| CN105145641B (en) * | 2015-10-26 | 2018-03-09 | 深圳市前海馥稷生物科技发展有限公司 | A kind of granule containing trichoderma harzianum and chitosan oligosaccharide |
| CN105165893A (en) * | 2015-10-27 | 2015-12-23 | 宁波三江益农作物保护有限公司 | Plant sterilization composite |
-
2017
- 2017-12-19 CN CN201711376304.8A patent/CN107969438B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107969438A (en) | 2018-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113785842A (en) | Pesticide composition for preventing and treating plant fungal diseases and preparation method thereof | |
| CN112889843B (en) | Killed rice-germ bacillus wettable powder for preventing and treating citrus red spiders and application thereof | |
| CN113662002A (en) | Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease | |
| CN103749542A (en) | Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof | |
| CN103858917A (en) | Mildew-resistant liquid used for seeds | |
| CN107969438B (en) | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof | |
| CN110074137A (en) | Application of the bacillus subtilis in prevention and treatment wheat aphid | |
| CN114617127A (en) | Pesticide sterilization composition and application thereof | |
| CN108041079B (en) | Wettable powder containing trichoderma harzianum T-22 and copper abietate and preparation method and application thereof | |
| CN103875718A (en) | Bacillus subtillis and hymexazol compounded soil treating agent | |
| CN114651825A (en) | Macadimia nut stem canker inhibitor based on molecular disease resistance approach | |
| CN110800754B (en) | Compound preparation for preventing and treating peach bacterial perforation disease and application | |
| CN103798262B (en) | A kind of bactericidal composition containing thiophanate-methyl and copper rosinate | |
| CN109090117B (en) | Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof | |
| CN112674103A (en) | Antibacterial composition, pesticide bactericide and method for preventing and treating diseases of crops | |
| CN104920358B (en) | Natural product combination for preventing and controlling rice blast | |
| CN108041057B (en) | Compound composition for preventing and treating fruit tree diseases and application thereof | |
| CN108651459A (en) | A method of the prevention water rice brown spot based on capsaicine | |
| CN115948301B (en) | Oligosaccharin composite microbial inoculant for preventing and controlling banana vascular wilt and application thereof | |
| CN113861088B (en) | Compound for preventing and treating plant bacterial diseases and application thereof | |
| CN108184903A (en) | A kind of chicken manure biogas slurry and bactericide compounded composition and the application in apple leaf diseases are prevented | |
| CN114391551B (en) | Novel fertilizer special for strawberries | |
| CN114190394B (en) | Fruit black spot resistant activator for macadimia nut breeding | |
| CN108990992B (en) | Sterilization composition containing pyraoxystrobin and mancozeb | |
| CN105941482A (en) | Compound pesticide containing Zhongshengmycin and Saisentong and application of compound pesticide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |