CN108419603A - The cultural method of selenium-rich gold needle mushroom - Google Patents

The cultural method of selenium-rich gold needle mushroom Download PDF

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Publication number
CN108419603A
CN108419603A CN201810187427.5A CN201810187427A CN108419603A CN 108419603 A CN108419603 A CN 108419603A CN 201810187427 A CN201810187427 A CN 201810187427A CN 108419603 A CN108419603 A CN 108419603A
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China
Prior art keywords
selenium
bottle
rich
needle mushroom
hollow cavity
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CN201810187427.5A
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Chinese (zh)
Inventor
李正宽
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CHONGQING KUANGE AGRICULTURAL DEVELOPMENT Co Ltd
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CHONGQING KUANGE AGRICULTURAL DEVELOPMENT Co Ltd
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Priority to CN201810187427.5A priority Critical patent/CN108419603A/en
Publication of CN108419603A publication Critical patent/CN108419603A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • A01G18/22Apparatus for the preparation of culture media, e.g. bottling devices
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/30Accessories for use before inoculation of spawn, e.g. sterilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/70Harvesting

Abstract

The invention belongs to the technical fields of agricultural planting, specifically disclose a kind of cultural method of selenium-rich gold needle mushroom, include the following steps:1. taking sawdust, yam flour, Se-rich wheat stalk, selenium-rich rice hulls, corn flour and lime;2. pulverizing and being stirred raw material, add water and be stirred, be eventually adding selenium-rich solution and jelly powder and be stirred;3. bottling, mixture is encased in culture bottle, culture bottle includes bottle, pedestal and sealing shroud, it is equipped with hollow cavity in pedestal, several rachises are rotatably connected in hollow cavity, the bottom of rachis is equipped with impeller, rachis is equipped with helical blade, helical blade is located in bottle, and escape pipe is communicated on sealing shroud, and escape pipe is connected to hollow cavity;4. base molded 5. inoculation strain is cultivated in sterilizing;6. bacteria;7. mycelium stimulation;8. it cultivates, it is primary every 8 hours Manual press sealing shrouds daily;9. harvesting.Selenium element is contained using the Ion Absorption of Flammulina Velutipes that this programme cultivates.

Description

The cultural method of selenium-rich gold needle mushroom
Technical field
The invention belongs to the technical fields of agricultural planting, specifically disclose a kind of cultural method of selenium-rich gold needle mushroom.
Background technology
Needle mushroom is free of chlorophyll, does not have photosynthesis, cannot manufacture carbohydrate, but completely can be in dark surrounds Middle growth, it is necessary to absorb ready-made organic substance from culture medium, such as the degradation product of carbohydrate, protein and fat, be Metatrophy type is a kind of heterotrophic organism, belongs to basidiomycetes.
Selenium is the essential trace elements of the human body, lacks selenium, and various diseases easily occur for human body.Containing needed by human in food Selenium can reduce the incidence of cancer, improve cardiac's situation, and generate beneficial effect to physical function.Flammulina velutipes mycelium has Higher selenium-rich conversion capability, the organic degree of selenium is high in selenium-rich mycelium, so needle mushroom is optimal one of the carrier of selenium.
Invention content
The purpose of the present invention is to provide a kind of cultural methods of selenium-rich gold needle mushroom.
In order to achieve the above object, base case of the invention is:The cultural method of selenium-rich gold needle mushroom, including following step Suddenly:
Step 1:Culture medium selection, is counted in parts by weight, takes sawdust 30-60, yam flour 25-35, Se-rich wheat stalk 20-35, selenium-rich rice hulls 5-10, corn flour 5-10 and lime 1-5;
Step 2:Culture medium is prepared, the raw material in step 1 is pulverized and carries out first time stirring, after stirring evenly Obtain powder;It is 1 that mass ratio is added into powder:1.5-1:1.6 water simultaneously carries out second of stirring, after stirring 30 minutes Obtain mixture;It is added into mixture and accounts for the selenium-rich solution of total weight 0.2-0.5% and the jelly powder of 2-8% and carry out third Secondary stirring is stirred 30 minutes;
Step 3:Bottling, mixture is encased in culture bottle, the culture bottle includes bottle, pedestal and can be set in On bottle and flexible sealing shroud, the bottle are fixed on pedestal, and hollow cavity is equipped in the pedestal, hollow One end of cavity is equipped with the first check valve, and the other end of hollow cavity is equipped with stomata, if being rotatably connected to cured leaf in hollow cavity The bottom of axis, rachis is equipped with impeller, and the top of hollow cavity and the bottom of bottle are equipped with through-hole, and rachis rotary seal is in through-hole Interior, rachis is equipped with helical blade, and helical blade is located in bottle;Escape pipe, escape pipe and institute are communicated on the sealing shroud Stomata connection is stated, sealing shroud is equipped with the second check valve;
Step 4:It sterilizes, cultivate base molded, carry out high-temperature sterilization after bottling, sterilising temp is 100 degrees Celsius, when sterilizing Between be 2-4h, wait for that culture medium is cooled to room temperature to form gel after sterilizing;
Step 5:Inoculation, strain is seeded on culture medium;
Step 6:Bacteria removes sealing shroud, and culture bottle is put into culturing room, keeps the air humidity of culturing room to be 85-90%, temperature are 15-17 degrees Celsius, are cultivated 20-30 days;
Step 7:Mycelium stimulation removes the mycelia of aging in culture bottle, then sealing shroud is set on bottle;
Step 8:It cultivates, indoor temperature will be cultivated and be maintained at 10-13 degree;Daily every 8 hours Manual press sealing shrouds Once;It is 20-40 days to cultivate the time;
Step 9:Harvesting, fructification harvest when growing to 14-15cm.
The working principle and beneficial effect of this base case is:
1. in step 1, having chosen one of which raw material of the Chinese yam as culture medium.Protein, grease and shallow lake in Chinese yam The content of powder is more than 70%, and a large amount of mineral matter element is also contained in Chinese yam, enough nutrients can be provided for needle mushroom.Simultaneously Also contain a large amount of mucoproteins in Chinese yam, mucoprotein has certain viscosity, so when all material is pulverized and water is added to obtain After mixture, mixture has certain viscosity.Culture medium in the prior art is Solid-liquid mixture mostly, after long-term standing, The raw material of solid can sink to the bottom of culture bottle, cause the nutrient of culture bottle bottom more, be unfavorable for needle mushroom and absorb nutrient. In the present solution, mixture has certain viscosity, mobility is poor, all raw material can be evenly distributed in culture bottle and It is not easy to precipitate.
2. in step 1, having chosen Se-rich wheat stalk and selenium-rich rice hulls as raw material, being both selenium-rich farming The waste of object, be not only able to for the cultivation of needle mushroom provide selenium source can also waste utilization, it is economic and environment-friendly.
3. adding selenium-rich solution in step 2, the Se content of culture medium is further improved.It is also added into pectin simultaneously Powder, jelly powder are a kind of gelling agents, and can increase the viscosity of liquid makes liquid become gel, so culture medium is in step 4 height Temperature is heated and can be become gel-like after cooling down.After culture medium becomes gel, all raw material and water are all generally evenly distributed in training It supports in base, greatly reduces that there is a situation where precipitate.The amount that jelly powder is added in this programme simultaneously only has 2-5%, culture medium will not Cause its viscosity too big because pectin powder content is high, needle mushroom can be unfavorable for instead and absorb nutrient.
4. mixture is encased in culture bottle in step 3.In step 8, have to cultivate when cultivating needle mushroom Bottle sealing, needle mushroom can release a large amount of carbon dioxide in growth course, so can accumulate many titanium dioxides in culture bottle Carbon can not be discharged, and be unfavorable for the growth of needle mushroom.In the prior art, culture bottle is sealed using the method for bagging, daily meeting Manually bag removal is taken a breath, it is very time-consuming and laborious.And in the present solution, seal culture bottle using the sealing shroud for having elasticity, After needle mushroom starts cultivation, one lower sleeve gasket of Manual press, the carbon dioxide in sealing shroud is only needed to be extruded in escape pipe, two For carbonoxide again from the hollow cavity that escape pipe enters pedestal, carbon dioxide can blow wheel rotation during flowing, Impeller drives rachis rotation, rachis to drive helical blade rotation, helical blade to stir culture medium, make nutrient in culture medium more Uniformly, final carbon dioxide can be discharged from the first check valve of hollow cavity.Sealing shroud can gradually restore to the original state after being pressed, Fresh air is absorbed from the external world to enter in culture bottle, absorb for needle mushroom by the second check valve.In base case, culture medium In gel, but acupuncture needle mushroom culture for a period of time after, the nutrient for cultivating epibasal tier is less, and lower layer's nutrient is more, is unfavorable for acupuncture needle Mushroom absorbs.And in the present solution, helical blade can stir culture medium, so that the nutrient in culture medium is uniformly mixed, and helical blade Agitation amplitude is small not to interfere with needle mushroom, while agitation can also ensure its stirring effect three times daily.
Preferred embodiment one:As the preferred of basic scheme, the sawdust includes the sawdust of willow and elm.Needle mushroom is certainly It can be generally grown on withered tree or the stub of willow and elm under right environment, so being conducive to gold using the sawdust of willow and elm The growth of needle mushroom.
Preferred embodiment two:As the preferred of basic scheme, in the step 2, the whipping temp of second of stirring is 30- 40 degree, the whipping temp of third time stirring is 10-15 degree.Water is added when second of stirring into powder, improves whipping temp It can be allowed to be sufficiently mixed.When third time stirs, jelly powder is added into mixture, jelly powder, which can play, becomes mixture The effect of gelled, but must first by mixed material heating again cooling could become gel, so being initially added into jelly powder When to reduce its whipping temp, prevent the too fast formation gel of mixture.
Preferred embodiment three:As the preferred of basic scheme, in the step 2, the additive amount of jelly powder is total to account for mixture Weight 5%.The additive amount of jelly powder will not both make the excessively high sticky mistake that will not make culture medium of viscosity of culture medium in this programme It is low.
Preferred embodiment four:As the preferred of basic scheme, contain sodium nitrite in the selenium-rich solution.Sodium nitrite helps Selenium element is absorbed in needle mushroom.
Preferred embodiment five:As the preferred of basic scheme, in step 8, sealing shroud was opened every 5 days when cultivation, to bottle Interior sprinkling selenium-rich solution.Needle mushroom is contributed to absorb selenium element using this programme.
Description of the drawings
Fig. 1 is the structural schematic diagram of culture bottle.
Specific implementation mode
Below by specific implementation mode, the present invention is described in further detail:
Reference numeral in Figure of description includes:Pedestal 1, hollow cavity 2, rachis 3, impeller 4, helical blade 5, first Check valve 6, the second check valve 7, sealing shroud 8, escape pipe 9, bottle 10.
The present embodiment, the cultural method of selenium-rich gold needle mushroom, includes the following steps:
Step 1:Culture medium selection, is counted in parts by weight, takes totally 30 parts of the sawdust of willow and elm, 30 parts of yam flour, richness 5 parts of 25 parts of selenium wheat stalk, 5 parts of selenium-rich rice hulls, 5 parts of corn flour and lime;Acupuncture needle is provided in culture medium raw materials Various elements needed for mushroom growth.
Step 2:Culture medium is prepared, the raw material in step 1 is pulverized and carries out first time stirring, after stirring evenly Obtain powder;It is 1 that mass ratio is added into powder:1.6 water simultaneously carries out second of stirring, 40 degree of whipping temp, stirring Mixture is obtained after 30 minutes;It is added into mixture and accounts for the selenium-rich solution of total weight 0.3% and 5% jelly powder and carry out the Triple mixing, 10 degree of whipping temp stir 30 minutes, wherein contain sodium nitrite in selenium-rich solution;
Step 3:Bottling, mixture is encased in culture bottle, as shown in Figure 1, culture bottle includes bottle 10,1 and of pedestal It can be set on bottle 10 and flexible sealing shroud 8, bottle 10 are fixed on pedestal 1, hollow cavity is equipped in pedestal 1 The left end of body 2, hollow cavity 2 is equipped with the first check valve 6, and the right end of hollow cavity 2 is equipped with stomata, and the interior rotation of hollow cavity 2 connects Several rachises 3 are connected to, the bottom of rachis 3 is equipped with impeller 4, and the top of hollow cavity 2 and the bottom of bottle 10 are equipped with through-hole, leaf For 3 rotary seal of axis in through-hole, rachis 3 is equipped with helical blade 5, and helical blade 5 is located in bottle 10;On the sealing shroud 8 It is communicated with escape pipe 9, escape pipe 9 is connected to the stomata, and sealing shroud 8 is equipped with the second check valve 7;
Step 4:It sterilizes, cultivate base molded, carry out high-temperature sterilization after bottling, sterilising temp is 100 degrees Celsius, when sterilizing Between be 3h, wait for that culture medium is cooled to room temperature to form gel after sterilizing;
Step 5:Inoculation, strain is seeded on culture medium;
Step 6:Bacteria removes sealing shroud 8, culture bottle is put into culturing room, keeps the air humidity of culturing room to be 90%, temperature is 15 degrees Celsius, is cultivated 20 days;
Step 7:Mycelium stimulation removes the mycelia of aging in culture bottle, then sealing shroud 8 is set on bottle 10;
Step 8:It cultivates, indoor temperature will be cultivated and be maintained at 12 degree;Daily every 8 hours Manual press sealing shrouds 8 one It is secondary;It is 30 days to cultivate the time, and sealing shroud 8 was opened every 5 days in cultivating process, and selenium-rich solution is sprayed into bottle 10;
Step 9:Harvesting, fructification harvest when growing to 14-15cm.
Above-described is only the embodiment of the present invention, and the common sense such as well known concrete structure and characteristic are not made herein in scheme Excessive description.It, without departing from the structure of the invention, can be with it should be pointed out that for those skilled in the art Several modifications and improvements are made, these should also be considered as protection scope of the present invention, these all do not interfere with what the present invention was implemented Effect and patent practicability.

Claims (6)

1. the cultural method of selenium-rich gold needle mushroom, which is characterized in that include the following steps:
Step 1:Culture medium selection, is counted in parts by weight, take sawdust 30-60, yam flour 25-35, Se-rich wheat stalk 20-35, Selenium-rich rice hulls 5-10, corn flour 5-10 and lime 1-5;
Step 2:Culture medium is prepared, the raw material in step 1 is pulverized and carries out first time stirring, is obtained after stirring evenly Powder;It is 1 that mass ratio is added into powder:1.5-1:1.6 water simultaneously carries out second of stirring, and stirring obtains after 30 minutes Mixture;It is added to account for the selenium-rich solution of total weight 0.2-0.5% and the jelly powder of 2-8% and carry out third time into mixture and stir It mixes, stirs 30 minutes;
Step 3:Bottling, mixture is encased in culture bottle, and the culture bottle includes bottle, pedestal and can be set in bottle Upper and flexible sealing shroud, the bottle are fixed on pedestal, and hollow cavity, hollow cavity are equipped in the pedestal One end be equipped with the first check valve, the other end of hollow cavity is equipped with stomata, several rachises, leaf is rotatably connected in hollow cavity The bottom of axis is equipped with impeller, and the top of hollow cavity and the bottom of bottle are equipped with through-hole, and rachis rotary seal is in through-hole, leaf Axis is equipped with helical blade, and helical blade is located in bottle;Escape pipe, escape pipe and the stomata are communicated on the sealing shroud Connection, sealing shroud are equipped with the second check valve;
Step 4:It sterilizes, cultivate base molded, carry out high-temperature sterilization after bottling, sterilising temp is 100 degrees Celsius, and sterilization time is 2-4h waits for that culture medium is cooled to room temperature to form gel after sterilizing;
Step 5:Inoculation, strain is seeded on culture medium;
Step 6:Bacteria removes sealing shroud, and culture bottle is put into culturing room, and it is 85- to keep the air humidity of culturing room 90%, temperature is 15-17 degrees Celsius, is cultivated 20-30 days;
Step 7:Mycelium stimulation removes the mycelia of aging in culture bottle, then sealing shroud is set on bottle;
Step 8:It cultivates, indoor temperature will be cultivated and be maintained at 10-13 degree;Daily every 8 hours Manual press sealing shrouds one It is secondary;It is 20-40 days to cultivate the time;
Step 9:Harvesting, fructification harvest when growing to 14-15cm.
2. the cultural method of selenium-rich gold needle mushroom according to claim 1, it is characterised in that:The sawdust includes willow and elm The sawdust of tree.
3. the cultural method of selenium-rich gold needle mushroom according to claim 1, it is characterised in that:In the step 2, second The whipping temp of stirring is 30-40 degree, and the whipping temp of third time stirring is 10-15 degree.
4. the cultural method of selenium-rich gold needle mushroom according to claim 1, it is characterised in that:In the step 2, jelly powder Additive amount be account for mixture total weight 5%.
5. the cultural method of selenium-rich gold needle mushroom according to claim 1, it is characterised in that:Contain Asia in the selenium-rich solution Sodium nitrate.
6. the cultural method of selenium-rich gold needle mushroom according to claim 1, it is characterised in that:In step 8, every 5 when cultivation It opens sealing shroud, and selenium-rich solution is sprayed into bottle.
CN201810187427.5A 2018-03-07 2018-03-07 The cultural method of selenium-rich gold needle mushroom Pending CN108419603A (en)

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CN109673391A (en) * 2019-02-26 2019-04-26 重庆市宽哥农业发展有限公司 A kind of culture medium and the method for cultivating zinc-rich elegant precious mushroom
CN109699397A (en) * 2019-02-26 2019-05-03 重庆市宽哥农业发展有限公司 A kind of culture medium and the method for cultivation of selenium-rich elegant precious mushroom
CN110226449A (en) * 2019-06-11 2019-09-13 宁洱大洋农业发展有限责任公司 A kind of dictyophora phalloidea implantation methods
CN110679391A (en) * 2019-09-16 2020-01-14 江苏华绿生物科技股份有限公司 Application of novel husk culture medium in industrial cultivation of white flammulina velutipes

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109220554A (en) * 2018-11-19 2019-01-18 遵义市播州区铃经纬香菇种植有限公司 A kind of monkey mushroom culture substrate formula
CN109673391A (en) * 2019-02-26 2019-04-26 重庆市宽哥农业发展有限公司 A kind of culture medium and the method for cultivating zinc-rich elegant precious mushroom
CN109699397A (en) * 2019-02-26 2019-05-03 重庆市宽哥农业发展有限公司 A kind of culture medium and the method for cultivation of selenium-rich elegant precious mushroom
CN110226449A (en) * 2019-06-11 2019-09-13 宁洱大洋农业发展有限责任公司 A kind of dictyophora phalloidea implantation methods
CN110679391A (en) * 2019-09-16 2020-01-14 江苏华绿生物科技股份有限公司 Application of novel husk culture medium in industrial cultivation of white flammulina velutipes

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