CN108410773B - Complex microbial inoculant and application thereof in remediation of organochlorine pesticide polluted water body - Google Patents
Complex microbial inoculant and application thereof in remediation of organochlorine pesticide polluted water body Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
Abstract
The invention belongs to the technical field of microorganisms, and discloses a compound microbial inoculum which comprises the following four bacteria: bacillus subtilis, Ochrobactrum anthropi, Phanerochaete chrysosporium, and Alcaligenes faecalis. The strains selected by the invention can form an efficient metabolic degradation system through a co-metabolic relationship, and compared with a single strain, the efficiency of degrading the organochlorine pesticide can be obviously improved.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a composite microbial inoculum and application thereof in remediation of organochlorine pesticide polluted water.
Background
The organic chlorine pesticide is an organic compound containing organic chlorine element in the composition for preventing and controlling plant diseases and insect pests. Mainly divided into two main categories of taking benzene as a raw material and taking cyclopentadiene as a raw material. The former is such as the first-used and most widely-used insecticides DDT and hexachloro cyclohexane, acaricide dicofol, dicofol and the like, the bactericides quintozene, chlorothalonil, daunomycin and the like; the latter is, for example, chlordane, heptachlor, aldrin, etc. as insecticides. In addition, camphene insecticide and toxaphene which take turpentine as raw materials and bornyl chloride which takes terpene as raw materials also belong to organochlorine pesticides. The common organic chlorine pesticide has the following characteristics: firstly, the liquid disappears slowly after being used; ② the fat solubility is strong; the chlorobenzene framework is stable, is not easy to degrade by in vivo enzyme and slowly disappears in organisms; fourthly, the product of the soil microbial action also has residual toxicity like the parent, such as DDD generated by reducing DDT and DDE generated by dehydrochlorination; some organochlorine pesticides, such as DDT, can be suspended on the water surface and can be evaporated along with water molecules. The organochlorine pesticide in the environment harms organisms through the action of biological enrichment and food chain. Acute toxicity to human is mainly to stimulate nerve center, and chronic poisoning is manifested by loss of appetite, weight loss, and sometimes cerebellar disorder, hematopoietic disorder, etc. The literature reports that some organochlorine pesticides have carcinogenicity on experimental animals. The chlorobenzene structure is stable, and the enzymes in organisms are difficult to degrade, so that the organochlorine pesticide molecules accumulated in animals and plants slowly disappear. Due to this property, it is further agroinjected and diffused by the action of bio-enrichment and food chain, the residual pesticides in the environment. Organochlorine pesticides that enter the human body through the food chain can accumulate in tissues such as liver, kidney, heart, etc., and particularly, because such pesticides are highly fat-soluble, the accumulation of organochlorine pesticides in body fat is more prominent. The accumulated pesticide residue can be discharged through breast milk or transferred into egg tissue, and affects the offspring.
The organochlorine pesticide can enter rivers and lakes along with rainwater, and causes pollution to the environment. At present, the adopted treatment methods mainly comprise chemical treatment and biological repair methods. Wherein, the chemical treatment method has quick response, but has higher cost and is easy to cause secondary pollution; compared with a chemical treatment method, the bioremediation method has much lower cost and no secondary pollution, but for a place which is seriously polluted by continuous organochlorine pesticides, the organochlorine pesticides are not easy to be used by single microorganisms, the microorganisms need to be compatible, and the microbial remediation method is influenced by various factors such as respective nutrition modes and metabolic modes of the microorganisms, so that the bacterial strains can be mixed to generate completely different effects, and the complex microbial inoculum with reasonable compatibility and mutual synergy is difficult to obtain, thereby the effect of quickly removing pollutants cannot be realized. Chinese invention patent 'CN 107364978A' discloses a method for removing an eluent organochlorine pesticide by adsorbing nano-iron and white-rot fungi, which enhances the survival and growth of the white-rot fungi in a synergistic eluent of a high-concentration surfactant by a degrading enzyme inducer, quickly degrades the surfactants such as Tween 80, Tween 20 and Triton X100 under the catalysis of the nano-iron by regulating nutrient components, forms adsorption on the organochlorine pesticide under the environment of proper temperature and the like, and has the removal efficiency of the eluent of the soil polluted by the high-concentration organochlorine pesticide reaching more than 90% within 10 days. The method adopts a chemical method to assist a biological method for treatment, has good effect, but has higher cost and is not easy to popularize and use. The development of a composite microbial inoculum which has low cost and high efficiency and can restore the water body polluted by the organochlorine pesticide is the technical problem to be solved.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a complex microbial inoculum; also provides the application of the composite microbial inoculum in repairing organochlorine pesticide polluted water.
The invention is realized by adopting the following technical scheme:
a complex microbial inoculum comprises the following four bacteria: bacillus subtilis, Ochrobactrum anthropi, Phanerochaete chrysosporium, and Alcaligenes faecalis.
Specifically, the preparation method of the complex microbial inoculum comprises the following steps:
uniformly mixing a bacillus subtilis-anthropi pallidus mixed fermentation liquid, a phanerochaete chrysosporium fermentation liquid and an alcaligenes faecalis fermentation liquid according to a volume ratio of 4-7:2-3:2-3 to obtain a composite bacterial liquid; and (3) mixing and stirring the compound bacterial liquid and the turfy soil uniformly according to the ratio of 1-5:1, and then freeze-drying to prepare the compound bacterial agent.
Preferably, the first and second electrodes are formed of a metal,
the preparation method of the bacillus subtilis-human ochrobactrum mixed fermentation liquor comprises the following steps:
inoculating bacillus subtilis seed liquid into a fermentation culture medium according to the inoculation amount of 6%, culturing for 6 hours at 30 ℃, then inoculating the ochrobactrum anthropi seed liquid into the fermentation culture medium according to the inoculation amount of 8%, and continuously culturing for 18 hours at 30 ℃ to obtain bacillus subtilis-ochrobactrum anthropi mixed fermentation liquid; the fermentation medium comprises the following components: 12g/L glucose, 5g/L yeast extract, 3g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.2mg/L chlordane.
Preferably, the first and second electrodes are formed of a metal,
the preparation method of the phanerochaete chrysosporium fermentation liquid comprises the following steps:
inoculating Phanerochaete chrysosporium seed liquid into a fermentation culture medium according to the inoculation amount of 10%, and culturing at 30 ℃ for 24h to obtain Phanerochaete chrysosporium fermentation liquid; the formula of the fermentation medium is as follows: 10g/L of molasses, 5g/L of soybean meal, 2g/L of urea, 0.1g/L of calcium chloride, 0.01g/L of manganese sulfate and 0.1mg/L of chlordane.
Preferably, the first and second electrodes are formed of a metal,
the preparation method of the alcaligenes faecalis fermentation liquid comprises the following steps:
inoculating the Alcaligenes faecalis seed solution into a fermentation culture medium according to the inoculation amount of 8% by volume, and culturing at 30 ℃ for 24h to obtain an Alcaligenes faecalis fermentation broth; the formula of the fermentation medium is as follows: 12g/L glucose, 5g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.1g/L sodium chloride, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.1mg/L chlordane.
Preferably, the first and second electrodes are formed of a metal,
the bacillus subtilis is ATCC39085, the human ochrobactrum anthropi is ATCC49188, the phanerochaete chrysosporium is ATCC34540, and the alcaligenes faecalis is ATCC 19209.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
the invention adopts four strains, has reasonable compatibility and good synergistic performance, can form an efficient metabolic degradation system through the co-metabolic relationship among the strains, and can obviously improve the efficiency of degrading the organochlorine pesticide compared with a single strain; the method for treating the pesticide wastewater by using the composite microbial inoculum has the advantages of simple and easy operation, low cost, environmental protection, no pollution and wide application prospect; the strain is firstly domesticated and cultured, so that the strain can adapt to the environment more quickly, and the efficiency of degrading chlordane is improved; through setting a plurality of groups of composite microbial inoculum tests, the composite microbial inoculum is processed for 6 hours, the existence of chlordane cannot be detected in a group with low concentration of 0.5mg/L, the complete degradation is realized, and the degradation effect is superior to that of a single microbial inoculum group and that of a composite microbial inoculum group of two strains.
Drawings
FIG. 1: the degradation effect of the compound microbial inoculum on chlordane is treated for 3 hours;
FIG. 2: the degradation effect of the compound microbial inoculum on chlordane is achieved after the compound microbial inoculum is treated for 6 hours.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the present application will be clearly and completely described below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from conventional biochemical sources, unless otherwise specified.
Example 1
A complex microbial inoculum comprises the following four strains: bacillus subtilis ATCC39085, Ochrobactrum anthropi ATCC49188, Phanerochaete chrysosporium ATCC34540, Alcaligenes faecalis ATCC 19209;
firstly, inoculating the bacillus subtilis seed liquid according to the inoculation amount of 6% (the inoculation density is 1 multiplied by 10)7CFU/ml) into a fermentation medium, culturing at 30 ℃ for 6 hours, and then inoculating the seed liquid of the human ochrobactrum according to the inoculation amount of 8% (the inoculation density is 0.5 multiplied by 10)7CFU/ml) is inoculated into a fermentation medium, and the mixture is continuously cultured for 18 hours at the temperature of 30 ℃ to obtain a bacillus subtilis-human ochrobactrum mixed fermentation liquid; the fermentation medium comprises the following components: 12g/L glucose, 5g/L yeast extract, 3g/L ammonium sulfate and dibasic phosphate1g/L potassium, 1g/L monopotassium phosphate, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.2mg/L chlordane;
inoculating Phanerochaete chrysosporium seed liquid at an inoculation amount of 10% (inoculation density of 1 × 10)7CFU/ml) is inoculated into a fermentation medium, and the culture is carried out for 24 hours at the temperature of 30 ℃ to obtain phanerochaete chrysosporium fermentation liquor; the formula of the fermentation medium is as follows: 10g/L of molasses, 5g/L of soybean meal, 2g/L of urea, 0.1g/L of calcium chloride, 0.01g/L of manganese sulfate and 0.1mg/L of chlordane.
Inoculating Alcaligenes faecalis seed liquid according to the inoculation amount of 8% volume ratio (the inoculation density is 1 multiplied by 10)7CFU/ml) is inoculated into a fermentation medium, and is cultured for 24 hours at 30 ℃ to obtain an alcaligenes faecalis fermentation broth; the formula of the fermentation medium is as follows: 12g/L glucose, 5g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.1g/L sodium chloride, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.1mg/L chlordane;
uniformly mixing a bacillus subtilis-anthropi pallidus mixed fermentation liquid, a phanerochaete chrysosporium fermentation liquid and an alcaligenes faecalis fermentation liquid according to a volume ratio of 4:2:3 to obtain a compound bacterial liquid;
and (3) mixing and stirring the compound bacterial liquid and the turfy soil uniformly according to the ratio of 1:1, and then freeze-drying to prepare the compound bacterial agent.
Example 2
A complex microbial inoculum comprises the following four strains: bacillus subtilis ATCC39085, Ochrobactrum anthropi ATCC49188, Phanerochaete chrysosporium ATCC34540, Alcaligenes faecalis ATCC 19209;
firstly, inoculating the bacillus subtilis seed liquid according to the inoculation amount of 6% (the inoculation density is 1 multiplied by 10)7CFU/ml) into a fermentation medium, culturing at 30 ℃ for 6 hours, and then inoculating the seed liquid of the human ochrobactrum according to the inoculation amount of 8% (the inoculation density is 0.5 multiplied by 10)7CFU/ml) is inoculated into a fermentation medium, and the mixture is continuously cultured for 18 hours at the temperature of 30 ℃ to obtain a bacillus subtilis-human ochrobactrum mixed fermentation liquid; the fermentation medium comprises the following components: 12g/L glucose, 5g/L yeast extract, 3g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.2mg/L chlordane;
inoculating Phanerochaete chrysosporium seed liquid at an inoculation amount of 10% (inoculation density of 1 × 10)7CFU/ml) is inoculated into a fermentation medium, and the culture is carried out for 24 hours at the temperature of 30 ℃ to obtain phanerochaete chrysosporium fermentation liquor; the formula of the fermentation medium is as follows: 10g/L of molasses, 5g/L of soybean meal, 2g/L of urea, 0.1g/L of calcium chloride, 0.01g/L of manganese sulfate and 0.1mg/L of chlordane.
Inoculating Alcaligenes faecalis seed liquid according to the inoculation amount of 8% volume ratio (the inoculation density is 1 multiplied by 10)7CFU/ml) is inoculated into a fermentation medium, and is cultured for 24 hours at 30 ℃ to obtain an alcaligenes faecalis fermentation broth; the formula of the fermentation medium is as follows: 12g/L glucose, 5g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.1g/L sodium chloride, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.1mg/L chlordane;
uniformly mixing a bacillus subtilis-anthropi pallidus mixed fermentation liquid, a phanerochaete chrysosporium fermentation liquid and an alcaligenes faecalis fermentation liquid according to a volume ratio of 7:3:2 to obtain a compound bacterial liquid;
and (3) mixing and stirring the compound bacterial liquid and the turfy soil uniformly according to the ratio of 2:1, and then freeze-drying to prepare the compound bacterial agent.
Example 3
The performance of the complex microbial inoculum is measured as follows:
putting chlordane into a reaction vessel containing 1 ton of water, respectively setting the concentration to be 0.5mg/L or 1mg/L, uniformly stirring, then adding 2g of the compound microbial inoculum, stirring at 100rpm for 3h and 6h under the condition of room temperature, and respectively detecting the concentration content of the chlordane;
the type of the complex microbial inoculum: group 1: example 1; group 2: the method of example 1 is otherwise the same except that the mixed fermentation broth of Bacillus subtilis and Ochrobactrum anthropi is used; group 3: the method of example 1 is followed except that the Phanerochaete chrysosporium fermentation broth is used; group 4: the method of example 1 is followed except that the Alcaligenes faecalis fermentation broth is used; group 5: the method of example 1 is the same as that of example 1 except that two kinds of bacterial liquids, i.e., phanerochaete chrysosporium fermentation liquid and alcaligenes faecalis fermentation liquid, are used.
As shown in fig. 1, the concentration of chlordane treated by the complex microbial inoculum of groups 1-5 is reduced in a group with low concentration of 0.5mg/L after the complex microbial inoculum is treated for 3 hours, wherein the most obvious reduction of the group 1 is 0.03mg/L, the group 3 and the group 4 using a single microbial inoculum have poor effect, and the degradation rate is about 60%; in the high concentration 1mg/L group, the group 1 has the best effect, after the treatment, the concentration of chlordane is reduced to 0.08mg/L, the single bacterium agent treatment effect is the worst, and the approximate trend is similar to that of the low concentration group. As shown in figure 2, the compound microbial inoculum is treated for 6 hours, and in the group with low concentration of 0.5mg/L, no chlordane exists in the group 1, so that complete degradation is realized; compared with the treatment time of 3h, the degradation effect of the chlordane is improved in other groups, but the complete degradation of the chlordane cannot be realized; and through the detection of the time point of 12h, the degradation efficiency of the composite microbial inoculum of each group on chlordane is not improved, but part of groups are reduced (not shown in the attached drawing), and through the counting observation of a microscope, part of strains die, possibly because the nutrient substances in water cannot meet the requirement of mass proliferation of the strains; the results show that the treatment time of the complex microbial inoculum is controlled within 6 h.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (1)
1. The compound microbial inoculum is characterized by comprising the following four bacteria: bacillus subtilis (A), (B) and (C)Bacillus subtilis ) Human Ochrobactrum anthropi: (A. anthropi.), (Ochrobactrum anthropi ) Phanerochaete chrysosporium (A)Phanerochaete chrysosporium ) Alcaligenes faecalis (A), (B), (C)Alcaligenes faecalis ） ；
The preparation method of the complex microbial inoculum comprises the following steps:
uniformly mixing a bacillus subtilis-anthropi pallidus mixed fermentation liquid, a phanerochaete chrysosporium fermentation liquid and an alcaligenes faecalis fermentation liquid according to a volume ratio of 4-7:2-3:2-3 to obtain a composite bacterial liquid; mixing the compound bacterial liquid and the turfy soil according to the ratio of 1-5:1, uniformly stirring, and then freeze-drying to prepare a compound microbial inoculum;
the preparation method of the bacillus subtilis-human ochrobactrum mixed fermentation liquor comprises the following steps:
inoculating bacillus subtilis seed liquid into a fermentation culture medium according to the inoculation amount of 6%, culturing for 6 hours at 30 ℃, then inoculating the ochrobactrum anthropi seed liquid into the fermentation culture medium according to the inoculation amount of 8%, and continuously culturing for 18 hours at 30 ℃ to obtain bacillus subtilis-ochrobactrum anthropi mixed fermentation liquid; the fermentation medium comprises the following components: 12g/L glucose, 5g/L yeast extract, 3g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.2mg/L chlordane;
the preparation method of the phanerochaete chrysosporium fermentation liquid comprises the following steps:
inoculating Phanerochaete chrysosporium seed liquid into a fermentation culture medium according to the inoculation amount of 10%, and culturing at 30 ℃ for 24h to obtain Phanerochaete chrysosporium fermentation liquid; the formula of the fermentation medium is as follows: 10g/L of molasses, 5g/L of soybean meal, 2g/L of urea, 0.1g/L of calcium chloride, 0.01g/L of manganese sulfate and 0.1mg/L of chlordane;
the preparation method of the alcaligenes faecalis fermentation liquid comprises the following steps:
inoculating the Alcaligenes faecalis seed solution into a fermentation culture medium according to the inoculation amount of 8% by volume, and culturing at 30 ℃ for 24h to obtain an Alcaligenes faecalis fermentation broth; the formula of the fermentation medium is as follows: 12g/L glucose, 5g/L ammonium sulfate, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 0.1g/L sodium chloride, 0.01g/L magnesium sulfate, 0.01g/L ferrous sulfate and 0.1mg/L chlordane;
the bacillus subtilis is ATCC39085, the human ochrobactrum anthropi is ATCC49188, the phanerochaete chrysosporium is ATCC34540, and the alcaligenes faecalis is ATCC 19209.
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| CN109604332B (en) * | 2019-02-20 | 2021-06-04 | 上海烟草集团有限责任公司 | Application of ochrobactrum intermedium in degradation of triadimefon |
| CN110257270B (en) * | 2019-03-22 | 2021-09-07 | 自然资源部第三海洋研究所 | Chromium-resistant petroleum hydrocarbon degrading bacterium Thp3-30 and application thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629172A (en) * | 2009-08-13 | 2010-01-20 | 浙江大学 | Method for preparing magnetic microbe immobilizing material for soil pollution repair and application thereof |
| CN103937722A (en) * | 2014-04-24 | 2014-07-23 | 烟台海上传奇生物科技有限公司 | Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent |
| CN104877950A (en) * | 2015-06-26 | 2015-09-02 | 黑龙江大学 | Bacillus pumilus capable of degrading atrazine efficiently |
| CN106478288A (en) * | 2016-09-18 | 2017-03-08 | 广西大学 | A kind of soil conditioner of utilization microbial degradation Pesticide Residue in Soil |
| CN107364978A (en) * | 2017-08-31 | 2017-11-21 | 中国科学院合肥物质科学研究院 | The method for removing eluent organo-chlorine pesticide using Nanoscale Iron and whiterot fungi absorption |
| CN107760632A (en) * | 2017-11-28 | 2018-03-06 | 浙江沃华环境科技有限公司 | A kind of complex micro organism fungicide and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060088563A1 (en) * | 2004-10-25 | 2006-04-27 | Board Of Trustees Of Michigan State University | Use of spray adjuvant to enhance the movement of pesticides through plant canopies to the target |
| CN105779344A (en) * | 2016-03-29 | 2016-07-20 | 江苏蓝星化工环保股份有限公司 | Composite microbial agent |
| CN106010565A (en) * | 2016-06-17 | 2016-10-12 | 战锡林 | Organochlorine pesticide contaminated soil remediation agent |
-
2018
- 2018-04-12 CN CN201810327416.2A patent/CN108410773B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629172A (en) * | 2009-08-13 | 2010-01-20 | 浙江大学 | Method for preparing magnetic microbe immobilizing material for soil pollution repair and application thereof |
| CN103937722A (en) * | 2014-04-24 | 2014-07-23 | 烟台海上传奇生物科技有限公司 | Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent |
| CN104877950A (en) * | 2015-06-26 | 2015-09-02 | 黑龙江大学 | Bacillus pumilus capable of degrading atrazine efficiently |
| CN106478288A (en) * | 2016-09-18 | 2017-03-08 | 广西大学 | A kind of soil conditioner of utilization microbial degradation Pesticide Residue in Soil |
| CN107364978A (en) * | 2017-08-31 | 2017-11-21 | 中国科学院合肥物质科学研究院 | The method for removing eluent organo-chlorine pesticide using Nanoscale Iron and whiterot fungi absorption |
| CN107760632A (en) * | 2017-11-28 | 2018-03-06 | 浙江沃华环境科技有限公司 | A kind of complex micro organism fungicide and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| Tourmaline combined with Phanerochaete chrysosporium to remediate agricultural soil contaminated with PAHs and OCPs;Wang C等;《J Hazard Mater》;20140115;第264卷;439-448 * |
| 几种污染环境下五氯酚的微生物修复研究;蒋晓云;《中国学位论文全文数据库》;20091106;1290-1295 * |
| 有机氯农药生物修复菌剂对雷竹生长及土壤化学性状的影响;郭子武等;《农业环境科学学报》;20100925;第29卷(第7期);全文 * |
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