CN104877950A - Bacillus pumilus capable of degrading atrazine efficiently - Google Patents
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Abstract
The invention provides bacillus pumilus capable of degrading atrazine efficiently and relates to bacillus capable of degrading the atrazine efficiently. The bacillus pumilus is bacillus pumilus gy 201401 and preserved in Common Microorganism Center, China Committee for Culture Collection of Microorganisms, the preservation address is No. 3, yard No. 1, Beichen West Rd, Chaoyang District, Beijing City, the preservation date is 29th, Dec, 2014, and the preservation number is CGMCC No. 10252. The bacillus pumilus belongs to a type of microorganisms which are great in stress resistance, the degradation of atrazine residual in soil is accelerated obviously after the bacillus pumilus is applied, harm of the atrazine residual to next crop of sensitive crops is alleviated or avoided, thereby the crop output is increased, and higher economic benefits are obtained.
Description
Technical field
The invention belongs to technical field of bioengineering, particularly relate to a kind of discovery can repairing the genus bacillus of atrazine-contaminated soil.
Background technology
G-30027 belongs to triazine herbicide, be before a kind of selectivity inner sucting conduction type seedling, herbicide after seedling, widely use in China in recent years, but it belongs to release weedicide, residence time is longer, easily ecotope is polluted, and it residual has serious poisoning to late stubble sensitive crop, be in agriculture production and ecotope a major issue.In recent years there is G-30027 to late stubble sensitive crop (beet, muskmelon, wheat, soybean etc.) hazardous events in China, directly causes serious financial loss time and again.
Microorganism is the biological group that degradable organic pollutant ability is stronger.Since nineteen eighty-two, scholars are successively separated the microorganism of the G-30027 that obtains degrading in bacterium, fungi and algae, and research is comparatively detailed, and that studies in actinomycetes is relatively less.The predominantly bacteria of the G-30027 of can degrading reported at present has Rhodopseudomonas (Pseudomonassp.), Alkaligenes (Alcaligens sp.), Agrobacterium (Agrobacterium sp.), acinetobacter (Acinetabactersp.), genus arthrobacter (Arthrobacter sp.), bacillus cereus (Bacillus cereus) etc.The main fungal of G-30027 of can degrading has microassembly robot (Pencillium janthinellum), red vertical mushroom (Hypholoma fasciculare), Aspergillus fumigatus (Aspergillus fumigatus), rhizopus stolonifer (Rhizopus stolonifer), yellow third aspergillus (Aspergillus flavipes), Penicillium decumbens (Penicillium decumbens) etc.
That studies microbiological deterioration G-30027 along with people gos deep into, and some problems also progressively manifest, and as in the lab, microorganism is fine to the degradation effect of G-30027, but but falls flat in environment, even contrary with testing laboratory effect; The toxicity problem etc. of the secondary metabolite of microbiological deterioration.Herein for the farmland pollution problem that G-30027 is residual, study its microbial degradation method, shaking flask acclimating method is adopted to separate degradation bacteria strains, application form, Physiology and biochemistry and molecular method carry out classification position qualification to the bacterial strain filtered out, specify growth characteristics and the nutritive property of degradation bacteria strains, simultaneously and adopt pot-culture method (to greatest extent access expansion environment) to evaluate the degradation effect of degradation bacteria strains.This research is G-30027 in degraded soil, alleviates its residual harm to second stubble crop, ensures crop production safety, alleviate the aspects such as ecological environmental pollution and all have great importance.
Summary of the invention
The invention provides the genus bacillus of a high-efficiency degradation G-30027, it is from soil, filter out G-30027 in soil of can degrading, alleviate or avoid G-30027 to remain the bacterial strain of the harm to lower stubble sensitive crop, thus reach the degraded of the G-30027 accelerating pedo relict, guarantee crop normal growth, ensure crop production safety, alleviate the objects such as ecological environmental pollution.
The bacillus pumilus of a high-efficiency degradation G-30027 of the present invention, it is bacillus pumilus (Bacilluspumilus) gy201401, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on December 29th, 2014, and preserving number is CGMCCNo.10252.
Bacillus pumilus of the present invention (Bacillus pumilus) gy201401 bacterial strain physiological and biochemical index is as shown in table 1.
Table 1 physiological and biochemical index
Molecular Identification: bacillus pumilus of the present invention (Bacillus pumilus) gy201401 bacterial strain is carried out 16SrDNA qualification, sequence length is 1576bp (as shown in sequence table SeqIDNo:1), its sequence is committed to GenBank, to determine the race relation of bacterial strain.Homology analysis result shows, the homology of the 16SrDNA sequence of this sequence and bacillus pumilus (Bacillus pumilus) is the highest, conserved regions similarity is 99%, by determining that it belongs to bacillus in conjunction with morphological features, growth conditions, Physiology and biochemistry qualification result, be bacillus pumilus (Bacillus pumilus).There are 100 sequences to be 99% with the similarity of acquisition sequence, are bacillus, wherein have 51 sequences to be bacillus pumilus (Bacillus pumilus).
By in the 16SrDNA sequence of bacterial strain and GenBank database, oneself knows that bacterial strain 16SrDNA sequence carries out sequence analysis, carries out alignment by CLUSTALX program, with MEGA5.05 software generation system evolutionary tree as shown in Figure 3.
Bacillus pumilus of the present invention (Bacillus pumilus) gy201401 degradation bacteria can well grow at 28 ~ 38 DEG C, and optimum growth temperature is 36 DEG C; Bacterial strain can grow in pH4 ~ 9, and optimal pH is 6.0; Air flow is larger, and thalli growth is better, illustrates that it is a strain aerobic bacteria.Its suitableeest carbon nitrogen source is respectively sucrose and extractum carnis.
Bacillus pumilus of the present invention (Bacillus pumilus) gy201401 bacterial strain is on beef-protein medium, and as shown in Figure 2, degradation bacteria bacterium colony is circular, edge sub-circular, smooth surface, and centre is yellow, opaque.
The present invention comprises following beneficial effect:
Bacillus pumilus of the present invention (Bacillus pumilus) gy201401 is the microorganism that a kind of resistance is very strong, the degraded of residual G-30027 in soil is obviously accelerated after using this microorganism, alleviate or avoid G-30027 and remain harm to lower stubble sensitive crop, thus improve the output of crop, obtain higher economic benefit.
The present invention obtains a strain by separation screening and can to degrade bacillus pumilus (Bacillus pumilus) gy201401 of G-30027, and it can arrive 36.14% to atrazine degradation rate.There is not been reported in the effect of this strains for degrading G-30027.In other report, find that Rhodopseudomonas, bacillus cereus, Aspergillus fumigatus, white-rot fungi and algae fibre etc. have Degradation to G-30027.
The present invention, by using this bacterium to pedo relict G-30027 and plant sensitive crop beet simultaneously, have rated bacillus pumilus (Bacillus pumilus) gy201401 further to the repair ability of G-30027 residual contamination in soil and the damaging effect alleviating or avoid crop.Research shows, when G-30027 residual quantity is respectively 30 μ g/kg, 60 μ g/kg, use this degradation bacteria, beet total solid yield increases by 24.42% and 60.98% respectively relative to the process not adding degradation bacteria, reduces by 1.5% and 4.71% respectively relative to blank; When G-30027 residual quantity is 90 μ g/kg, the beet total solid yield adding this degradation bacteria increases by 413.46% relative to the process not adding degradation bacteria, reduces by 15.32% relative to blank.As can be seen here, under this test conditions, bacillus pumilus (Bacillus pumilus) gy201401 is applied in the residual soil of lower concentration G-30027, poisoning effect can be eliminated completely, and high density G-30027 (90 μ g/kg) the poisoning effect to beet can be alleviated.From the pedo relict G-30027 amount once measured, its residual quantity is all less than 50 μ g/kg, therefore, bacillus pumilus (Bacillus pumilus) gy201401 has G-30027 in efficient degradation soil, alleviates and even avoids G-30027 to remain toxic action to crop.
Accompanying drawing explanation
Fig. 1 is the acquisition schema of degradation bacteria gy201401;
Fig. 2 is the colonial morphology of degradation bacteria gy201401 on beef-protein medium;
Fig. 3 is the phylogeny tree graph of degradation bacteria gy201401;
Fig. 4 is degradation bacteria gy201401BLAST comparison result figure;
Fig. 5 is the upgrowth situation front view of 14 days beet seedlings in different concns G-30027 remains of emerging;
Fig. 6 is the upgrowth situation vertical view of 14 days beet seedlings in different concns G-30027 remains of emerging;
Fig. 7 is the upgrowth situation front view of 18 days beet seedlings in different concns G-30027 remains of emerging;
Fig. 8 is the upgrowth situation vertical view of 18 days beet seedlings in different concns G-30027 remains of emerging;
Fig. 9 is the upgrowth situation front view of 26 days beet seedlings in different concns G-30027 remains of emerging;
Figure 10 is the upgrowth situation vertical view of 26 days beet seedlings in different concns G-30027 remains of emerging.
Embodiment
Embodiment one: the bacillus pumilus of a high-efficiency degradation G-30027 of present embodiment, it is bacillus pumilus (Bacillus pumilus) gy201401, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on December 29th, 2014, and preserving number is CGMCCNo.10252.
Content of the present invention is not limited only to the content of the respective embodiments described above, and the combination of one of them or several embodiment equally also can realize the object of inventing.
Bacillus pumilus (Bacillus pumilus) gy201401 of present embodiment is (screening process figure is as shown in Figure 1) of carrying out in the following manner screening:
One, tame: in soil (experimental plot, school district, Heilongjiang University Hulan is not by the black earth of pollution of herbicide), add 10000 times of G-30027s, temperature be 30 DEG C, humidity be the condition of 70% under cultivate one month;
Two, enrichment: get the 5g soil sample after step one domestication, being added to containing concentration is in the 100mL beef extract-peptone liquid nutrient medium of the G-30027 of 100mg/L, is then placed in 250mL triangular flask, 160r/min shaking culture 72h in 36 DEG C of shaking culture casees;
Three, transfer: by the nutrient solution after step 2 enrichment by 10% switching amount to be added to containing concentration be in the G-30027 basic inorganic salt liquid nutrient medium of 100mg/L (G-30027 makes only nitrogen source), in 250mL triangular flask, add the above-mentioned substratum of 100mL, then temperature be 36 DEG C, rotating speed be the condition of 160r/min under shaking culture (under culture condition with); Every 72h gets 10% nutrient solution and is inoculated in fresh minimal medium, and the G-30027 content simultaneously in each minimal medium cultivated all increases 100mg/L, and continuous domestication is cultivated until G-30027 final concentration is increased to 600mg/L;
Four, solid medium is selected to cultivate: bacterium liquid step 3 finally cultivated does gradient dilution, respectively get 100 μ L to the bacterium liquid of different gradient to coat on basic inorganic salt solid medium flat board (using G-30027 as only nitrogen source), cultivate in 36 DEG C of constant incubators;
Five, filter out aimed strain: according to bacterium colony mode of appearance, pick out the bacterium colony that mode of appearance is different, these different bacterium colonies are distinguished purifying three times, obtains purifying bacterial strain.
Six, the purifying bacterial strain obtained is carried out degradation rate mensuration: the purifying bacterial strain obtained is prepared into bacteria suspension, being inoculated into containing concentration is in the 50mL basic inorganic salt liquid nutrient medium of 200mg/L G-30027, in 36 DEG C of shaking culture casees after 160r/min shaking culture 72h, the residual quantity of G-30027 is detected with high performance liquid chromatography, recording degradation rate is after 36.14%, collects bacterial strain; Wherein, liquid phase chromatogram condition: chromatographic column (25cm × 4.6mm (id)) stainless steel column, inside fills out C18 (5 μm), temperature room temperature, determined wavelength 222nm, moving phase is methyl alcohol: water=55:45, flow velocity, 0.7mL/min, appearance time is about 17.3min.
The bacterial strain of collection is carried out 16SrDNA qualification, and sequence length is 1576bp (as shown in sequence table Seq ID No:1), and its sequence is committed to GenBank, to determine the race relation of bacterial strain.Homology analysis result shows, the homology of the 16SrDNA sequence of this sequence and bacillus pumilus (Bacillus pumilus) is the highest, conserved regions similarity is 99%, by determining that it belongs to bacillus in conjunction with morphological features, growth conditions, Physiology and biochemistry qualification result, be bacillus pumilus (Bacillus pumilus).There are 100 sequences to be 99% with the similarity of acquisition sequence, are bacillus, wherein have 51 sequences to be bacillus pumilus (Bacillus pumilus).
By analyzing above and can determining, the bacterial strain of screening is bacillus pumilus (Bacillus pumilus), we are by its called after bacillus pumilus (Bacillus pumilus) gy201401, and preservation is carried out to it, concrete preservation information is: bacillus pumilus (Bacillus pumilus) gy201401, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on December 29th, 2014, and preserving number is CGMCC No.10252.
Described basic inorganic salt culture medium component is: K
2hPO
40.5g, KH
2pO
40.5g, MgSO
40.2g, NaCl0.2g, glucose 5.0g, G-30027 is only nitrogen source, distilled water 1000mL, pH7.0 ± 0.2.
Functional verification is carried out to bacillus pumilus (Bacillus pumilus) the gy201401 bacterial strain that above-mentioned screening obtains:
Bacillus pumilus (Bacillus pumilus) gy201401 degradation bacteria is eliminated the poisoning of G-30027 beet:
1, test method
The atrazine concentration that this test is administered to soil is respectively 0,30,60,90,120 μ g/kg, successively the liquid prepared evenly is sprayed on soil from low to high according to pesticide concentration, soil and liquid is made to puddle evenly, the soil prepared inoculates bacillus pumilus (Bacillus pumilus) gy201401 bacterial strain respectively by 2% inoculum size, establish simultaneously and add G-30027 and do not add the process of bacterial strain and do not add the clear water process of G-30027, each process repeats for 3 times, supplement appropriate moisture content every 3 days, keep certain water content.Static cultivation is carried out one week at 25 DEG C.
The beet variety that this test is selected is that bus is gloomy, select the seed of full grains, presoaking and germinating in thermostat container after sterilization, after 3 days, every basin selects 12 beet seed kinds of having sprouted in cultured soil, cultivate in illumination cultivation room, culture condition: day temperature 25 DEG C, nocturnal temperature 19 DEG C, humidity 60%, light radiation intensity 420 μm of olm
-2s
-1, illumination 14h/ days.
Observe emerging and situation of growing of beet, measure seedling rate and mortality ratio.
Seedling rate=(beet is emerged and counted/sowing number) × 100%;
Mortality ratio=(beet death toll/number of emerging) × 100%;
26 days sampling and measuring amount of dry matters, chlorophyll content and Net Photosynthetic Rate after emerging.
Measuring chlorophyll content instructs with reference to plant physiology experiment.
Photosynthetic rate (Pn) adopts the portable photosynthetical system analysis-e/or determining of LCA-4.
2, conclusion (of pressure testing)
2.1, G-30027 and bacillus pumilus (Bacillus pumilus) gy201401 degradation bacteria emerging and the impact of survival condition on beet.
Test-results is found out, blank seedling rate is 97.22%, the seedling rate of G-30027 process is followed successively by 97.22%, 94.44%, 94.44%, 94.44% by concentration order from low to high, is vaccinated with the seedling rate after degradation bacteria gy201401 and is respectively 94.44%, 94.44%, 97.22%, 97.22%.Difference is not obvious compared with the control in the process different through variance analysis.This shows that the different concns G-30027 of experimental design does not have a significant effect to emerging of beet; Have an impact the adding also not emerge to beet of bacterium liquid.Therefore, under this test conditions, degradation bacteria and G-30027 also do not affect emerging of beet.
By Fig. 5 ~ 10 known (Y2 in figure refers to bacillus pumilus (Bacillus pumilus) gy201401), although Te Lajin and degradation bacteria on the impact of seedling rate not quite, As time goes on start to have an impact to the growth of seedling in Ah's soil.Emerge 14 days time, in soil, G-30027 residual quantity is that in 60 μ g/kg process, poisoning is also not obvious; But in 90 μ g/kg process, do not use existing most of beet seedling in bacillus pumilus (Bacillus pumilus) gy201401 process dead, and there are not the beet seedling phenomena of mortality in the process of using bacillus pumilus (Bacillus pumilus) gy201401.
Emerge 18 days time, in soil, G-30027 residual quantity is in 60 μ g/kg process, can find out poisoning on beet seedling growth create impact; In 90 μ g/kg process, the beet seedling growth of not using bacillus pumilus (Bacillus pumilus) gy201401 process is subject to serious suppression, and the beet seedling growth of using bacillus pumilus (Bacillus pumilus) gy201401 process is only subject to slight restraining effect.
Emerge 26 days time, in soil, G-30027 residual quantity is in 60 μ g/kg process, obviously can find out the poisoning phenomenon of G-30027 to beet, use the process of bacillus pumilus (Bacillus pumilus) gy201401 and not obvious simultaneously; In 90 μ g/kg process, the beet seedling not using bacillus pumilus (Bacillus pumilus) gy201401 process does not almost grow, and the beet seedling using bacillus pumilus (Bacillus pumilus) gy201401 process is compared with contrast, grow fine.
2.2, G-30027 and bacillus pumilus (Bacillus pumilus) gy201401 degradation bacteria are on the impact of the seedling dry matter of beet.
2.2.1 G-30027 is on the impact of the seedling dry matter of beet.
G-30027 poisoning results from pot experiment test shows, the not impact of emerging of using beet of G-30027, but has very large harm to beet seedling, and emerge latter 26 days to during results, when atrazine concentration is 120 μ g/kg, beet seedling is all dead; When atrazine concentration is 90 μ g/kg, the mortality ratio of beet seedling is 86.11%; Even if the G-30027 of lower concentration does not cause beet seedling dead, but seriously reduces sugar beet seedlings bio yet.Beet is when G-30027 residual concentration is 30 μ g/kg, 60 μ g/kg and 90 μ g/kg, and total solid yield reduces by 18.42%, 40.81% and 83.51% respectively relative to blank.
2.2.2 bacillus pumilus (Bacillus pumilus) gy201401 uses the seedling dry accumulation of rear each process beet.
When G-30027 residual quantity is 30 μ g/kg, the beet total solid yield adding bacillus pumilus (Bacillus pumilus) gy201401, relative to the beet total solid yield 24.42% not adding bacillus pumilus (Bacillus pumilus) gy201401 process, increases by 1.50% relative to blank.Using of bacillus pumilus (Bacillus pumilus) gy201401 completely eliminates the poisoning effect of G-30027 to beet.
When G-30027 residual quantity is 60 μ g/kg, the beet total solid yield adding bacillus pumilus (Bacillus pumilus) gy201401, relative to the beet total solid yield 60.98% not adding bacillus pumilus (Bacillus pumilus) gy201401 process, distinguishes 4.71% relative to blank.Using of bacillus pumilus (Bacillus pumilus) gy201401 almost eliminates the poisoning effect of G-30027 to beet.
When G-30027 residual quantity is 90 μ g/kg, the beet total solid yield adding bacillus pumilus (Bacillus pumilus) gy201401, relative to the beet total solid yield 413.46% not adding bacillus pumilus (Bacillus pumilus) gy201401 process, reduces by 15.32% relative to blank.Bacillus pumilus (Bacillus pumilus) gy201401 uses, and can alleviate the poisoning effect of G-30027 to beet.
2.3, G-30027 and bacillus pumilus (Bacillus pumilus) gy201401 are on the photosynthetic impact of beet
2.3.1, G-30027 and bacillus pumilus (Bacillus pumilus) gy201401 are on the impact of beet leaf chlorophyll content
Beet is when G-30027 residual concentration is 30 μ g/kg, 60 μ g/kg and 90 μ g/kg, and Chlorophyll content reduces 5.34%, 8.75% and 11.86% respectively relative to blank.
When G-30027 residual quantity is 30 μ g/kg, the beet Chlorophyll content adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 6.10% relative to the beet total solid yield not adding degradation bacteria process, increases by 0.44% relative to blank; When G-30027 residual quantity is 60 μ g/kg, the beet Chlorophyll content adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 9.54% relative to the beet total solid yield not adding bacillus pumilus (Bacillus pumilus) gy201401 process, reduces by 0.04% relative to blank; When G-30027 residual quantity is 90 μ g/kg, the beet Chlorophyll content adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 12.48% relative to the beet Chlorophyll content not adding bacillus pumilus (Bacillus pumilus) gy201401 process, reduces by 0.86% relative to blank.
Illustrate thus, G-30027 is residual is formed with restraining effect to beet is chlorophyllous, bacillus pumilus (Bacilluspumilus) gy201401 is when G-30027 residual quantity is 30 μ g/kg and 60 μ g/kg, eliminate the restraining effect of G-30027 to beet chlorophyll formation, alleviate restraining effect when 90 μ g/kg.
2.3.2, G-30027 and bacillus pumilus (Bacillus pumilus) gy201401 are on the impact of beet leaf Net Photosynthetic Rate
Beet is when G-30027 residual concentration is 30 μ g/kg, 60 μ g/kg and 90 μ g/kg, and the Net Photosynthetic Rate of beet reduces 2.18%, 10.13% and 16.76% respectively relative to blank.
When G-30027 residual quantity is 30 μ g/kg, the beet Net Photosynthetic Rate adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 2.55% relative to the beet Net Photosynthetic Rate not adding bacillus pumilus (Bacillus pumilus) gy201401 process, increases by 0.31% relative to blank; When G-30027 residual quantity is 60 μ g/kg, the beet Net Photosynthetic Rate adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 8.50% relative to the beet Net Photosynthetic Rate not adding bacillus pumilus (Bacillus pumilus) gy201401 process, reduces by 2.49% relative to blank; When G-30027 residual quantity is 90 μ g/kg, the beet Net Photosynthetic Rate adding bacillus pumilus (Bacillus pumilus) gy201401 increases by 7.77% relative to the beet Net Photosynthetic Rate not adding bacillus pumilus (Bacillus pumilus) gy201401 process, reduces by 10.29% relative to blank.
Illustrate thus, G-30027 remains has restraining effect to the Net Photosynthetic Rate of beet, bacillus pumilus (Bacilluspumilus) gy201401 is when G-30027 residual quantity is 30 μ g/kg, eliminate the restraining effect of G-30027 to beet Net Photosynthetic Rate, alleviate restraining effect when 60 μ g/kg and 90 μ g/kg.
Bacillus pumilus (Bacillus pumilus) gy201401 uses the pollution residual to G-30027 and has significant repair, and bacillus pumilus (Bacillus pumilus) gy201401 uses normal total solid yield, chlorophyll content and the net photosynthetic rate that can ensure sensitive crop beet.Under this test conditions, bacillus pumilus (Bacillus pumilus) gy201401 is applied in the residual soil of lower concentration G-30027, poisoning effect can be eliminated completely, and high density G-30027 (90 μ g/kg) the poisoning effect to beet can be alleviated.
Claims (1)
1. the bacillus pumilus of a high-efficiency degradation G-30027, it is characterized in that it is bacillus pumilus (Bacilluspumilus) gy201401, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on December 29th, 2014, and preserving number is CGMCCNo.10252.
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Cited By (6)
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| CN105802874A (en) * | 2015-11-03 | 2016-07-27 | 山东鲁虹肥料研究院 | Mixed bacterium for efficiently degrading atrazine and fermental culture method |
| CN108118018A (en) * | 2018-02-09 | 2018-06-05 | 广东海洋大学 | One plant of A Shi bacillus Bacillus aryabhattai W-5 and its application |
| CN108410773A (en) * | 2018-04-12 | 2018-08-17 | 孙雪华 | A kind of composite bacteria agent and its application in repairing organochlorine pesticide pollution water body |
| CN109136104A (en) * | 2018-09-12 | 2019-01-04 | 华东理工大学 | One Aspergillus oryzae and its application |
| CN114958802A (en) * | 2022-03-18 | 2022-08-30 | 上海市农业科学院 | Hydrolase from bacillus elongatus and method for repairing atrazine pollution of soil by using hydrolase |
| CN116004453A (en) * | 2022-12-15 | 2023-04-25 | 黑龙江八一农垦大学 | Atrazine degrading strain, fermentation liquor and application thereof |
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| CN105802874A (en) * | 2015-11-03 | 2016-07-27 | 山东鲁虹肥料研究院 | Mixed bacterium for efficiently degrading atrazine and fermental culture method |
| CN105802874B (en) * | 2015-11-03 | 2019-11-19 | 山东鲁虹智慧农业研究院 | A kind of Mixed Microbes and fermentation culture method of efficient degradation Atrazine |
| CN108118018A (en) * | 2018-02-09 | 2018-06-05 | 广东海洋大学 | One plant of A Shi bacillus Bacillus aryabhattai W-5 and its application |
| CN108118018B (en) * | 2018-02-09 | 2020-10-02 | 广东海洋大学 | Bacillus aryabhattai W-5 and application thereof |
| CN108410773A (en) * | 2018-04-12 | 2018-08-17 | 孙雪华 | A kind of composite bacteria agent and its application in repairing organochlorine pesticide pollution water body |
| CN108410773B (en) * | 2018-04-12 | 2021-06-08 | 杭州富阳佳畅机械有限公司 | Complex microbial inoculant and application thereof in remediation of organochlorine pesticide polluted water body |
| CN109136104A (en) * | 2018-09-12 | 2019-01-04 | 华东理工大学 | One Aspergillus oryzae and its application |
| CN109136104B (en) * | 2018-09-12 | 2021-03-26 | 华东理工大学 | Aspergillus oryzae and application thereof |
| CN114958802A (en) * | 2022-03-18 | 2022-08-30 | 上海市农业科学院 | Hydrolase from bacillus elongatus and method for repairing atrazine pollution of soil by using hydrolase |
| CN116004453A (en) * | 2022-12-15 | 2023-04-25 | 黑龙江八一农垦大学 | Atrazine degrading strain, fermentation liquor and application thereof |
| CN116004453B (en) * | 2022-12-15 | 2023-09-01 | 黑龙江八一农垦大学 | Atrazine degrading strain, fermentation liquor and application thereof |
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