CN105062917A - Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof - Google Patents
Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof Download PDFInfo
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- CN105062917A CN105062917A CN201510440030.9A CN201510440030A CN105062917A CN 105062917 A CN105062917 A CN 105062917A CN 201510440030 A CN201510440030 A CN 201510440030A CN 105062917 A CN105062917 A CN 105062917A
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Abstract
The invention discloses a chloroacetamide herbicide degrading strain, a bacterium produced thereby and application thereof. The chloroacetamide herbicide degrading strain, rhodococcus sp. AC-1, is preserved in China General Microbiological Culture Collection Center under CGMCC No.10778 on 30th April, 2015. The chloroacetamide herbicide degrading strain AC-1 is suitable for use in degrading chloroacetamide herbicides, preferably in degrading chloroacetamide herbicides in soil; by directly applying a degrading strain product, residue of the chloroacetamide herbicides (specifically alachlor, acetochlor and butachlor) in the soil can be decreased by above 85%, the problem that the residue of the alachlor, acetochlor and butachlor in agricultural production is out of limits is solved, and chemical damage caused by herbicide residue to crops is prevented.
Description
Technical field
The invention belongs to biological high-tech field, relate to microbial inoculum and the application of a kind of chloro-acetyl amine herbicide degradation bacterial strain and production thereof.
Technical background
Chemical pesticide must the indispensable important production means as modern agriculture, for protecting agriculture is produced, ensured that China and even world food have played safely vital effect.But because China has a large population and a few land, farmland multiple crop index is high, chemical pesticide exceeds standard use in a large number, and due to pesticide application technology backwardness, agricultural chemicals utilization ratio only 20% ?about 30%, 70% ?80% agricultural chemicals enter soil and water, cause soil and Residual Pesticides in Farm Produce seriously polluted, the healthy and ecological environment security of harm humans, affects the foreign exchange earning of agricultural products in China.Along with the raising of people's living standard and the enhancing of environmental protection consciousness, the direct harm that the whole society brings pesticide residue and potential impact are also more and more paid close attention to, the clean green agricultural product that people pollute in the urgent need to non agricultural chemical residuum.The agricultural form current according to China and demographic situation, it is impracticable for reducing to stop Pesticide use the No-harmful apple orchard pattern that farm output is cost, and continuation is also played huge effect as the effective means of disease and pest control and controlling weeds by agricultural chemicals in agriculture production.This double-barreled question how solved in agriculture production just becomes the study hotspot of every subjects.Microorganism is the main force of contaminant degradation in environment, utilize the microbiological deterioration pesticide residue eliminated in soil carry out in-situ immobilization be a kind of economy, safety, the method for effective and non-secondary pollution, have broad application prospects.
CN104480043A discloses rhodococcus (Rhodococcussp.) 198 ?R ?56CGMCCNo.9868 and reaches 84.63% to the degradation rate of 100mg/L Pu Shite in 7 days in minimal medium, 69.15% is reached to 50mg/L acetochlor degradation rate, 52.20% is reached to 100mg/L chlorimuronethyl degradation rate, 20.16% is reached to the degradation rate that 100mg/L weeds are burnt.But it is lower to the degradation rate of acetochlor, consuming time longer, and do not carry out the degradation experiment in soil, be unfavorable for applying large Tanaka.
Summary of the invention
The object of the invention is to for the practical problems in production practice and demand, develop a kind of novel bacterium for degradating residual agricultural chemical, use this microbial inoculum that the residual quantity of alachlor, acetochlor and Butachlor technical 92 three kinds of chloro-acetyl amine weedicides can be made to reduce by more than 85%.
Object of the present invention realizes by following technical scheme:
The invention provides a kind of chloro-acetyl amine herbicide degradation strains A C ?1, for gram positive bacterium, through being accredited as Rhod (Rhodococcussp.), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCCNo.10778, and preservation date is on April 30th, 2015.
Strains A C ?1 morphological feature: strains A C ?1 on LB flat board in pale pink bacterium colony, bacterium colony is protruding, and surface wettability is smooth, neat in edge, opaque, and acetochlor flat board can be formed transparent degraded circle (Figure 1A).Under transmission electron microscope strains A C ?1 in shaft-like (0.5 ?0.9 μm × 3.5 ?4.5 μm), atrichia (Figure 1B).
Strains A C ?1 physiological and biochemical property: aerobic, can not move, gramstaining is positive, and catalase, urase are positive, and oxydase, amylolytic enzyme and nitrate reductase be feminine gender.Strains A C ?1 can be by alachlor, acetochlor and Butachlor degradation 2 ?Lv ?N ?(2 ?Yi Ji ?6 ?aminomethyl phenyl) ethanamide.The suitableeest degraded substrate is acetochlor, and under laboratory shake flask culture condition, the degradation rate of acetochlor reaches more than 98% (Fig. 2).This bacterium can produce by the general fermentation equipment of fermentation industry.
The technique using above-mentioned organophosphorus pesticide degradation bacteria to produce microbial inoculum is: inclined-plane kind-shaking flask kind-seeding tank-production tank-product (packaging formulation is liquid bacterial agent or solid absorption microbial inoculum).
Chloro-acetyl amine herbicide degradation strains A C described in use ?1 produce a microbiological deterioration microbial inoculum, be produce by the following method:
1) chloro-acetyl amine herbicide degradation strains A C ?1 test tube slant kind be inoculated in LB culture media shaking vase, shaking culture is to logarithmic phase;
2) by above-mentioned cultured bacterial classification by 10% inoculum size inoculate into seeding tank, be cultured to logarithmic phase, seeding tank culture medium prescription used is: peptone 5g/L, yeast extract paste 2.5g/L, K
2hPO
41g/L, NaCl5g/L, CaCO
32g/L, soybean oil 0.1% (v/v), pH value 7.2 ?7.5;
3) seed liquor is by the inoculum size access production tank cultivation of 10%, produces tank used medium identical with seed tank culture base;
4) seeding tank and produce tank culturing process in the air flow of sterile air be 1:0.6 ?1.2, stirring velocity is 180-240 rev/min, culture temperature is 30 DEG C, whole process incubation time be 96 ?108 hours, after fermentation ends, thalline quantity reaches 1,000,000,000/more than mL, and the rear nutrient solution that fermented goes out tank and is directly distributed into liquid dosage form by plastic barrel or packing bottle or adopts adsorption by peat packing bag to be distributed into solid fungicide formulation.
Chloro-acetyl amine herbicide degradation strains A C of the present invention ?1 application in degradating chloro acetyl herbicide, the application preferably in degraded soil in chloro-acetyl amine weedicide.
Wherein, the described preferred alachlor of chloro-acetyl amine weedicide, acetochlor or Butachlor technical 92, further preferred acetochlor.
The application of microbiological deterioration microbial inoculum of the present invention in degradating chloro acetyl herbicide, the application preferably in degraded soil in chloro-acetyl amine weedicide.
Wherein, the described preferred alachlor of chloro-acetyl amine weedicide, acetochlor or Butachlor technical 92, further preferred acetochlor.
Beneficial effect
The invention provides a strain can efficiently, the bacterium AC of fast degradation chloro-acetyl amine weedicide ?1.Degradation bacteria AC ?1 there is wider degraded spectrum, can in 48 the alachlor of degradable 0.2mM, acetochlor and Butachlor technical 92, have a wide range of applications potentiality and value.It is low, easy to use that the degradation bacterial agent using this this bacterium to produce has production and application cost, the advantage that removal effect is good, is adapted at national grain oil & vegetable production export base or has the local spread of green food brand mark to use.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value improving agricultural-food has great importance.This degradation bacterial agent is used before crop seeding, normally to use chemical pesticide carry out control of weeds and ensure that Residual Pesticides in Farm Produce content meets green food requirement.
Accompanying drawing explanation
Fig. 1 strains A C ?1 bacterium colony photo (A) and electromicroscopic photograph (B)
Fig. 2 strains A C ?the degraded of 1 pair of chloro-acetyl amine weedicide
Fig. 3 temperature and pH metal ion on degradation bacteria AC ?1 impact of degrading acetochlor
Fig. 4 metal ion on degradation bacteria AC ?1 impact of degrading acetochlor
Fig. 5 inoculum size on strains A C ?1 impact of degrading acetochlor
Fig. 6 strains A C ?1 degrade acetochlor meta-bolites LC ?MS identify collection of illustrative plates
Wherein scheme the liquid chromatogram of A. meta-bolites; Figure B. retention time is the mass spectrum that 13.49min material is corresponding; Figure C. retention time is the mass spectrum that 7.56min material is corresponding.
Biomaterial preservation information
AC ?1, Classification And Nomenclature is rhodococcus Rhodococcussp., be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCCNo.10778, preservation date is on April 30th, 2015, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Embodiment
The Isolation and ldentification of embodiment 1, bacterial strain
Contain at 100mL in the liquid inorganic salt culture medium (hereinafter referred to as MM) of 0.2mM acetochlor and add 5g acetochlor contaminated soil sample, 30 DEG C, 150rpm shaking table cultivation 7d, be forwarded in fresh same substratum with 5% inoculum size (v/v), continuous enrichment culture four times.By the 5th generation pregnant solution dilution spread on the MM solid medium containing 0.2mM acetochlor, cultivate 5d for 30 DEG C, single bacterium colony on picking flat board, be forwarded in 3mLLB test tube and cultivate, collected by centrifugation thalline, being forwarded to 20mL contains in the MM substratum of 0.2mM acetochlor, cultivate 5d for 30 DEG C, by heavy molten with 5mL methyl alcohol after nutrient solution lyophilize, UV scanning and high performance liquid chromatography (HPLC) is used to detect the degradation effect of single bacterium colony, finally separate a strain acetochlor degradation bacteria strains AC ?1, delivered the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, culture presevation number is CGMCCNo.10778, preservation date is on April 30th, 2015.
Strains A C ?1 on LB flat board in pale pink bacterium colony, bacterium colony is protruding, and surface wettability is smooth, neat in edge, opaque, and acetochlor (concentration is greater than 300ppm) flat board can be formed transparent degraded circle (Figure 1A).Under transmission electron microscope strains A C ?1 in shaft-like (0.5 ?0.9 μm × 3.5 ?4.5 μm), atrichia (Figure 1B).Strains A C ?1 physiological and biochemical property be: aerobic, can not move, gramstaining is positive, and catalase, urase are positive, and oxydase, amylolytic enzyme and nitrate reductase be feminine gender.By strains A C ?1 16SrRNA gene order compare of analysis in database EzBilCloud, result display strains A C ?1 belongs to sibship recently, wherein with R.erythropolisDSM43066 with Rhodococcus
tsimilarity reaches 99.93%, with R.jialingiaedjl ?6 ?2
tsimilarity reaches 99.49%.In conjunction with colonial morphology characteristic, physiological and biochemical property and 16SrRNA gene compare of analysis, strains A C ?1 be initially identified as Rhodococcus belong to.
Embodiment 2, laboratory degradation experiment
2.1 seed liquor preparations
By strains A C ?1 access in the 100mLLB substratum containing 0.2mM acetochlor, 30 DEG C, 150rpm shaking table cultivates, 6000rpm collected by centrifugation thalline after 24h, uses MM to wash thalline twice, finally uses 10mLMM resuspended, for subsequent use as seed liquor.
2.2 strains A C ?the degraded of 1 pair of chloro-acetyl amine weedicide
By strains A C ?1 by 5% inoculum size be connected in the 100mLMM containing 0.2mM alachlor, acetochlor, Butachlor technical 92 and metolachlor respectively, 30 DEG C, 150rpm shaking table cultivate, every 8 hours sampling 5mL, be taken to 48 hours.Detect alachlor, acetochlor, Butachlor technical 92 and metolachlor residual quantity, calculate degradation rate, draw strains A C ?1 pair of chloro-acetyl amine weedicide Shi Jian ?degradation curve.As Fig. 2, bacterial strain energy AC ?1 in 48h the acetochlor of degradable 0.2mM, to alachlor and Butachlor technical 92, also there is good degradation effect.But, strains A C ?the degradation effect of 1 pair of metolachlor very weak.
2.3 temperature and pH on strains A C ?1 impact of degrading acetochlor
Be in the basal salt media of 0.2mM at interpolation acetochlor final concentration, by 5% inoculum size access strains A C ?1 seed liquor, respectively under 15,20,25,30,35 and 40 DEG C of conditions, 150rpm shaking table is cultivated, after 24 hours, sampling detects Residual Determination of Acetochlor, calculate degradation rate, determine temperature on strains A C ?1 impact of degrading acetochlor.Shown in Fig. 3 (A), strains A C ?1 the highest to acetochlor degradation rate 30 DEG C time, 25 ?within the scope of 35 DEG C, acetochlor of all well degrading, degradation rate is all more than 80%; When temperature is lower than 15 DEG C, strains A C ?the degradation rate of 1 pair of acetochlor drop to less than 40%.
Be respectively in the MM substratum of 6.0,6.5,7.0,7.5,8.0,8.5 and 9.0 at initial pH, add the acetochlor of 0.2mM, by 5% inoculum size access strains A C ?1 seed liquor, in 30 DEG C, the cultivation of 150rpm shaking table, after 24 hours, sampling detects Residual Determination of Acetochlor, calculate degradation rate, determine pH on strains A C ?1 impact of degrading acetochlor.Not inoculate degradation bacteria strains for contrast.Shown in Fig. 3 (B), strains A C ?1 best to the degradation effect of acetochlor when pH7.5; PH6.5 ?8.5 scope in all to degrade preferably acetochlor; And when pH be less than 6 or be greater than 9 time, its degradation capability obviously declines, and degradation rate is lower than 45%.
2.4 metal ions on degradation bacteria AC ?1 impact of degrading acetochlor
Be in the MM substratum of 0.2mM at interpolation acetochlor final concentration, add the metal ion (Mg of 0.1mM
2+, Co
2+, Cu
2+, Li
+, Hg
2+, Ni
2+, Ca
2+, Mn
2+, Fe
2+and Zn
2+), by 5% inoculum size access strains A C ?1 seed liquor, contrast (connect bacterium but do not add any metal ion) is set simultaneously, in 30 DEG C, the cultivation of 150rpm shaking table, after 24 hours, sampling detects Residual Determination of Acetochlor, calculate degradation rate, determine metal ion on strains A C ?1 impact of degrading acetochlor.Shown in Fig. 4, compared with the control, the Cu of 0.1mM
2+and Hg
2+to strains A C ?1 acetochlor of degrading there is very strong restraining effect, degradation rate is compared with the control lower than 20%; The Co of 0.1mM
2+, Ni
2+, Mn
2+, Ca
2+and Li
+to strains A C ?1 acetochlor of degrading there is partial inhibition; The Mg of 0.1mM
2+and Zn
2+on strains A C ?1 acetochlor of degrading do not affect; And the Fe of 0.1mM
2+then to strains A C ?1 acetochlor of degrading there is faint promoter action.
2.5 inoculum sizes on strains A C ?1 impact of degrading acetochlor
As shown in Figure 5, along with the increase of inoculum size, strains A C ?1 speed of degrading acetochlor also accelerate, when inoculum size is 0.5%, strains A C ?1 still can not the acetochlor of degradable 0.2mM after 56h, and when inoculum size reaches 10%, strains A C ?1 can degradable acetochlor in 32h.
2.6 strains A C ?1 degrade acetochlor meta-bolites LC ?MS identify collection of illustrative plates
By strains A C ?1 meta-bolites of degrading acetochlor carry out HPLC ?MS qualification.As shown in Fig. 6 (A), there are two peaks in sample in liquid chromatogram, and retention time (RT) is respectively 13.49min and 7.56min; Fig. 6 (B) represents that retention time is the mass spectrum of the material of 13.49min, and its M/Z is 270.13 [M]
+, consistent with acetochlor molecular weight, and acetochlor molecule is with a chlorine atom, therefore there will be isotopic peak 272.18 [M]
+; Fig. 6 (C) is depicted as the mass spectrum that retention time is the material of 7.56min, and its M/Z is 212.23 [M]
+, with 2 ?Lv ?N ?(2 ?Yi Ji ?6 ?aminomethyl phenyl) molecular weight of ethanamide is consistent.With 2 ?Lv ?N ?(2 ?Yi Ji ?6 ?aminomethyl phenyl) ethanamide be substrate continue cultivate strains A C ?1, find bacterial strain can not continue this substrate of degrading.Therefore, we infer strains A C ?1 pathways metabolism of degrading acetochlor: namely acetochlor sloughs the alkyl in ethoxymethyl side chain, be converted into 2 ?Lv ?N ?(2 ?Yi Ji ?6 ?aminomethyl phenyl) ethanamide.
Embodiment 3, soil degrading are tested
Take vegetable garden soil as supplying examination soil sample.Soil sample is crossed 2mm sieve, get a certain amount of alachlor, acetochlor and Butachlor technical 92 pulvis respectively and be dissolved in 10mL methyl alcohol, then soak diatomite, agricultural chemicals is adsorbed completely.Diatomite after immersion is placed in stink cupboard and dries up, and is admixed in soil, makes the concentration of Pesticide Residue in Soil be about 1mg/kg.Each soil sample is respectively got 500g and is cultivated in 30 DEG C of constant incubators, and by the inoculum size access seed liquor of 10%, not connect bacterium in contrast, the water holding capacity of soil remains on 60%.Cultivate after 7 days, HPLC measures residual quantity.Measurement result is as table 1.
As can be drawn from Table 1, cultivated through 7 days, strains A C-1 reaches 86.1%, 90.1% and 86.3 respectively to the degradation rate of alachlor, acetochlor and Butachlor technical 92.These results suggest that, strains A C-1 in being manured into soil after, do not have to occur not degrading or phenomenon that degradation efficiency sharply declines, its degradation property is stablized, and this just provides scientific basis for strains A C-1 to alachlor, acetochlor and the reparation of Butachlor technical 92 contaminated soil.
Table 1 strains A C ?1 in soil to the degraded of chloro-acetamide agricultural chemicals
The technique using above-mentioned chloro acetochlor class herbicide residue degradation bacteria to produce microbial inoculum is: inclined-plane kind-shake-flask seed liquid-seeding tank-product (packaging formulation is liquid bacterial agent).
Detailed implementation step of the present invention is:
By chloro-acetyl amine herbicide degradation bacterium AC of the present invention ?1 original seed activate on culture dish, and measure degradation property, be inoculated on test tube slant for subsequent use.Test tube kind is inoculated in the 1000mL shaking flask containing 200mLLB substratum (LB culture medium prescription: peptone 10g, yeast powder 5g, sodium-chlor 5g, water 1L, pH7.4), and constant-temperature shaking culture, to logarithmic phase, prepares inoculation first class seed pot.First class seed pot 50L, charging capacity 40L, culture medium prescription is: peptone 5g/L, yeast extract paste 2.5g/L, K
2hPO
41g/L, NaCl5g/L, CaCO
32g/L, soybean oil 0.1% (v/v), pH value 7.2 ?7.5.121 DEG C of high pressure moist heat sterilizations after feeding intake, after being cooled to 30 DEG C, by above-mentioned cultured shaking flask bacterial classification by 10% inoculum size inoculate into 50L first class seed pot, be cultured to logarithmic phase, stirring velocity is 220 revs/min, and sterile air intake is 1:0.8.The seed liquor arriving logarithmic phase is pressed the inoculum size access secondary seed tank of 10%.Secondary seed tank 500L, charging capacity 400L, culture medium prescription is consistent with first class seed pot with culture condition.By arriving the seed liquor of logarithmic phase by the inoculum size access production tank cultivation of 10%, produce tank used medium composition identical with seed tank culture base.Produce tankage 5 tons, charging capacity 4 tons.Production tank 1.1kg/cm after feeding intake
2pressure under, 121 DEG C of high pressure moist heat sterilizations, are cooled to 30 DEG C after sterilizing, logical sterile air keeps sterile state for subsequent use.Postvaccinal production tank temperature controls at 30 DEG C, and producing the air flow of sterile air in the culturing process of tank is 1:0.8-1.0, and stirring velocity is 240 revs/min, whole technical process incubation time be 96 ?108 hours.After fermentation ends, thalline quantity reaches 1,000,000,000/more than mL.
The rear nutrient solution that fermented goes out tank and is directly distributed into liquid dosage form by plastic barrel or packing bottle or adopts adsorption by peat packing bag to be distributed into solid fungicide formulation.
Embodiment 4
By strains A C ?1 be seeded in 100mL LB liquid medium, 30 DEG C, 180rpm cultivates 24h, centrifugal segregation LB substratum, bacterium uses and MM is resuspended and by OD
600be adjusted to 1.0.Draw 0.2mL bacteria suspension (1 × 10
9cfu/mL) be seeded to 5mL and contain (altogether 21 test tubes) in the MM test tube of 50mg/L acetochlor, 28 DEG C, 180rpm cultivation.Three test tubes are got every 1 day, measure Residual Determination of Acetochlor as follows: often prop up test tube and get 3mL degradation solution in 50mL centrifuge tube, the centrifugal 5min of 8000rpm collects supernatant liquor, add 3mL methylene dichloride, concuss 5min, leave standstill 10min, treat aqueous phase and organic phase layering, draw organic phase and add a small amount of anhydrous sodium sulphate and organic phase is dewatered, accurate absorption 800 μ L organic phase is in 1.5mL centrifuge tube, dry up with Nitrogen evaporator, add 400 μ l methyl alcohol, ultrasonic 10min in ultrasonic cleaner, detect to HPLC with after the organic phase frit impurity of 0.22 μm.Blank process: meanwhile, with non-inoculating strain AC ?1 the above-mentioned MM containing 50mg/L acetochlor in contrast, measure the concentration of acetochlor according to the method described above.
Result shows, and acetochlor starting point concentration is 42.23mg/L, and after 3 days, described strains A C ?1 pair of acetochlor degradation rate reaches 65.26%, reaches 99.43% after 5 days, and after 6 days, then inspection does not measure.Result show strains A C ?1 degradation effect be much better than rhodococcus (Rhodococcus) 198 ?R ?56CGMCCNo.9868.
Claims (8)
1. a chloro-acetyl amine herbicide degradation strains A C-1, it is characterized in that this microorganism is the Rhod (Rhodococcussp.) of Gram-positive, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCCNo.10778, and preservation date is on April 30th, 2015.
2., with the microbiological deterioration microbial inoculum that chloro-acetyl amine herbicide degradation strains A C-1 according to claim 1 produces, it is characterized in that producing by the following method:
1) chloro-acetyl amine herbicide degradation strains A C-1 test tube slant kind is inoculated in LB culture media shaking vase, and shaking culture is to logarithmic phase;
2) by above-mentioned cultured bacterial classification by 10% inoculum size inoculate into seeding tank, be cultured to logarithmic phase, seeding tank culture medium prescription used is: peptone 5g/L, yeast extract paste 2.5g/L, K
2hPO
41g/L, NaCl5g/L, CaCO
32g/L, soybean oil 0.1% (v/v), pH value 7.2-7.5;
3) seed liquor is by the inoculum size access production tank cultivation of 10%, produces tank used medium identical with seed tank culture base;
4) in the culturing process of seeding tank with production tank, the air flow of sterile air is 1:0.6-1.2, stirring velocity is 180-240 rev/min, culture temperature is 30 DEG C, whole process incubation time is 96-108 hour, after fermentation ends, thalline quantity reaches 1,000,000,000/more than ml, and the rear nutrient solution that fermented goes out tank and is directly distributed into liquid dosage form by plastic barrel or packing bottle or adopts adsorption by peat packing bag to be distributed into solid fungicide formulation.
3. the application of chloro-acetyl amine herbicide degradation strains A C-1 according to claim 1 in degradating chloro acetyl herbicide.
4. application according to claim 3, is characterized in that the application of chloro-acetyl amine herbicide degradation strains A C-1 according to claim 1 in degraded soil in chloro-acetyl amine weedicide.
5. the application according to claim 3 or 4, is characterized in that described chloro-acetyl amine weedicide is alachlor, acetochlor or Butachlor technical 92.
6. the application of microbiological deterioration microbial inoculum according to claim 2 in degradating chloro acetyl herbicide.
7. application according to claim 6, is characterized in that the application of microbiological deterioration microbial inoculum according to claim 2 in degraded soil in chloro-acetyl amine weedicide.
8. the application according to claim 6 or 7, is characterized in that described chloro-acetyl amine weedicide is alachlor, acetochlor or Butachlor technical 92.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420172A (en) * | 2016-01-08 | 2016-03-23 | 南京农业大学 | Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria |
| CN109136097A (en) * | 2018-07-12 | 2019-01-04 | 农业部环境保护科研监测所 | The penicillium oxalicum of degradation isopropyl methoxalamine and its application |
| CN109439573A (en) * | 2018-10-30 | 2019-03-08 | 南京农业大学 | There is bacterial strain, hydroamidase, encoding gene and its application of single-minded transformation function to S- napropamide |
| CN110982707A (en) * | 2019-12-23 | 2020-04-10 | 中国科学院沈阳应用生态研究所 | Odontoglossum rugoso-annulata and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122312A (en) * | 2013-02-01 | 2013-05-29 | 南京农业大学 | Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition |
| CN104480043A (en) * | 2014-12-12 | 2015-04-01 | 中国农业科学院农业资源与农业区划研究所 | Rhodococcus sp. capable of degrading four herbicides and application thereof |
-
2015
- 2015-07-23 CN CN201510440030.9A patent/CN105062917B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122312A (en) * | 2013-02-01 | 2013-05-29 | 南京农业大学 | Microorganism composition for degrading acetochlor and/or butachlor and application of microorganism composition |
| CN104480043A (en) * | 2014-12-12 | 2015-04-01 | 中国农业科学院农业资源与农业区划研究所 | Rhodococcus sp. capable of degrading four herbicides and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| FEI WANG ET AL.: "Involvement of the Cytochrome P450 System EthBAD in the N-Deethoxymethylation", 《AEM》 * |
| HONG-MING LIU ET AL.: "Biodegradation of Butachlor by Rhodococcus sp. Strain B1 and Purification of Its Hydrolase (ChlH) Responsible for N‑Dealkylation ofChloroacetamide Herbicides", 《 BIODEGRADATION OF BUTACHLOR BY RHODOCOCCUS SP. STRAIN B1 AND PURIFICATION OF ITS HYDROLASE (CHLH) RESPONSIBLE FOR N‑DEALKYLATION OFCHLOROACETAMIDE HERBICIDES》 * |
| Y. HOU ET AL.: "Degradation of acetochlor by a bacterial consortium of Rhodococcus sp.T3-1, Delftia sp.T3-6 and Sphingobium sp.MEA3-1", 《APPLIED MICROBIOLOGY》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420172A (en) * | 2016-01-08 | 2016-03-23 | 南京农业大学 | Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria |
| CN105420172B (en) * | 2016-01-08 | 2018-12-14 | 南京农业大学 | Microbial inoculum and the application of a kind of metribuzin pesticide residual degrading bacteria and its production |
| CN109136097A (en) * | 2018-07-12 | 2019-01-04 | 农业部环境保护科研监测所 | The penicillium oxalicum of degradation isopropyl methoxalamine and its application |
| CN109136097B (en) * | 2018-07-12 | 2019-10-29 | 农业农村部环境保护科研监测所 | The penicillium oxalicum of degradation isopropyl methoxalamine and its application |
| CN109439573A (en) * | 2018-10-30 | 2019-03-08 | 南京农业大学 | There is bacterial strain, hydroamidase, encoding gene and its application of single-minded transformation function to S- napropamide |
| CN109439573B (en) * | 2018-10-30 | 2021-06-01 | 南京农业大学 | Bacterial strain with specific conversion function on S-napropamide, amidohydrolase, coding gene and application thereof |
| CN110982707A (en) * | 2019-12-23 | 2020-04-10 | 中国科学院沈阳应用生态研究所 | Odontoglossum rugoso-annulata and application thereof |
| CN110982707B (en) * | 2019-12-23 | 2023-03-31 | 中国科学院沈阳应用生态研究所 | Odontoglossum rugoso-annulata and application thereof |
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