CN105861318A - Alterneria tenuissima and application thereof in biological weeding - Google Patents
Alterneria tenuissima and application thereof in biological weeding Download PDFInfo
- Publication number
- CN105861318A CN105861318A CN201610229836.8A CN201610229836A CN105861318A CN 105861318 A CN105861318 A CN 105861318A CN 201610229836 A CN201610229836 A CN 201610229836A CN 105861318 A CN105861318 A CN 105861318A
- Authority
- CN
- China
- Prior art keywords
- application
- tenuissima
- strain
- alterneria
- alternaria tenuissima
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an Alterneria tenuissima strain and application thereof in biological weeding. The classified name of the strain is Alterneria tenuissima, the complete name of the strain is Alterneria tenuissima HZ-1, the strain is preserved in the China General Microbiological Culture Collection Center located at the No.3 room, No.1 yard, Beichen west road, Chaoyang district, Beijing city, China on January 11, 2016, and the preservation number is CGMCC No.11992. The Alterneria tenuissima is used for biological weeding of broad-leaved weeds such as galium tricorne, chenopodium album and malva verticillata in a highland barley field, a broad bean field and a pea field and can effectively control and prevent the broad-leaved weeds.
Description
Technical field
The application belongs to field of ecology, specifically, relate to a strain Alternaria tenuissima and
Application in biological weed control.
Background technology
Crop smothering is one of main factor causing crop failure, causes Global Agriculture every year
95000000000 dollars of losses.Now, farmland weed controls or based on chemical herbicide.
Along with being widely used of chemical herbicide, the negative effect brought is the most obvious.Along with
The raising of agricultural modernization level, continuation is increased by the herbicide usage amount in global range.
Use chemical herbicide in a large number and not only bring environmental pollution crisis, and cause lower stubble to be made
The thing underproduction even land deterioration.Existing more than the 100 kind chemical herbicides in the whole world are at more than 30
Country is disabled or cancels registration.It addition, the whole world has been found that 324 of 188 kinds of weeds
Bion creates the resistance to the action of a drug to 19 class chemical herbicides so that drug effect reduces, dosage
Increase, cost improves, and has the most more increased the weight of to pollute.Consequence is to cause the list of herbicide kind
One changes, also enhance market to develop wide spectrum, efficiently, low toxicity, to non-target organism and
The biological weed killer of mammalian safe and the demand of development biological weed control technology.Utilize certainly
So active microorganism and metabolite thereof in boundary's biology, develop biological weed killer
Substitute or part substitutes chemical herbicide, be the important channel solving this contradiction.Relatively
It is up to the development cost of 100,000,000 dollars in each new chemical herbicide, develops biological weed control
The cost of agent will low decades of times even hundreds of times.
Since the eighties in 20th century, the biological weed killer that the whole world is the most successfully developed
Product has more than 20 kinds, in the market can biological weed killer about 11.
Wherein Biochon, StumpOut, Camperico, bialaphos sodium and
Myco-TechPaste has become as international product.Although biological weed killer product exists
Ratio escheat in weed control technology is the lowest, but utilizes microorganism and metabolite conduct thereof
Herbicide has become as the developing direction of this field industrialization.According to world environments and development
May require that greatly, the 60% of yield agricultural chemicals the to be accounted for total output of whole world biological pesticide.But,
For biological weed control, although reach far away this target at present, but make great efforts development biology
Weeding technique has been increasingly becoming the common recognition in the whole world.
Summary of the invention
In view of this, the application is for the problems referred to above, it is provided that a strain Alternaria tenuissima and
Application in biological weed control.
In order to solve above-mentioned technical problem, this application discloses a strain Alternaria tenuissima, its point
The named Alternaria tenuissima of class, Alternaria tenuissima, this bacterial strain was in 2016 01
The moon is preserved in No. 3 China Microbiological bacterium of North Star West Road 1, Chaoyang District, city of BeiJing, China institute on the 11st
Planting preservation administration committee common micro-organisms center, preserving number is CGMCC No.11992.
Disclosed herein as well is the preparation side of the ferment filtrate of a kind of above-mentioned Alternaria tenuissima
Method, comprises the following steps: the colony edge after cultivating 7d takes mycelia block and breaks into a diameter of 5mm
Bacterium cake, is inoculated in 200mLPDB nutrient solution, every bottle graft 5 pieces, 25 DEG C of 180r min-1Training
Supporting 5d, nutrient solution vacuum filtration, filtrate is again with 0.45 μm filtering with microporous membrane, filter of must fermenting
Liquid.
Disclosed herein as well is the application biological weed control of a kind of above-mentioned Alternaria tenuissima.
Further, the ferment filtrate of this bacterial strain is sprayed on highland barley, broad bean or pea,
Plant performance safety.
Further, ferment filtrate prepares particular by following methods: cultivating 7d
After colony edge take mycelia block and break into a diameter of 5mm bacterium cake, be inoculated into 200mLPDB
In nutrient solution, every bottle graft 5 pieces, 25 DEG C of 180r min-1Cultivation 5d, nutrient solution vacuum filtration,
Filtrate with 0.45 μm filtering with microporous membrane, obtains ferment filtrate again.
Further, the amount of spraying of ferment filtrate is 50mL/m2。
Further, grass is broad leaved weeds.
Further, broad leaved weeds is clearvers, cottonsedge-like thistle herb, lamb's-quarters, winter certain herbaceous plants with big flowers or sorrel
One or several in knotweed.
Compared with prior art, the application can obtain and include techniques below effect:
1) present invention utilizes microbial resources management of weeds to have affects little, environment to crop
Negative effect is few, security advantages of higher, meets the development of sustainable agriculture, by generation
The great attention of various countries of boundary.Evaluation to microbial herbicide, in theory Main Basis two
Bar standard i.e. validity (drug effect) and selectivity (security).Weeds biocontrol microorganisms possesses both to target
Mark weeds have the most pathogenic two major features comparatively safe to crop again.
2) the present invention is directed to the broad-leaveds such as highland barley, broad bean, the clearvers in pea field, lamb's-quarters, winter certain herbaceous plants with big flowers
Weeds, a kind of toVerticilliumdahliaActivitie Activitie S of Relative bacteria strain of screening, for biological weed control.The present invention can effectively pacify
Entirely controlling and prevent and treat part broad leaved weed, low cost, pollution-free, low-residual is environmentally friendly.
Certainly, the arbitrary product implementing the application must be not necessarily required to reach above-described simultaneously
All technique effects.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing further understanding of the present application, constitutes the application
A part, the schematic description and description of the application is used for explaining the application, not structure
The improper restriction of the application in pairs.In the accompanying drawings:
Fig. 1 is the morphological feature of the application Alternaria tenuissima HZ-1, wherein I: bacterium colony shape
State；II: hypha form；III: spore；
Fig. 2 is the bacterial strain HZ-1 that the application builds based on ITS rDNA sequence B LAST result
Phylogenetic tree；
Fig. 3 is that the application Alternaria tenuissima HZ-1 is to pale persicaria, cottonsedge-like thistle herb, winter certain herbaceous plants with big flowers
With the in vitro causative effect of lamb's-quarters, wherein, 1-4 is respectively pale persicaria, cottonsedge-like thistle herb, winter certain herbaceous plants with big flowers
With lamb's-quarters control group；1 '-4 ' it is respectively pale persicaria, cottonsedge-like thistle herb, winter certain herbaceous plants with big flowers and lamb's-quarters HZ-1 process
Group；
Fig. 4 is that the application Alternaria tenuissima HZ-1 is to clearvers, lamb's-quarters, winter certain herbaceous plants with big flowers and sorrel
The potted plant causative effect of knotweed, wherein, 1-4 is respectively clearvers, lamb's-quarters, winter certain herbaceous plants with big flowers and pale persicaria
Control group；1 '-4 ' it is respectively clearvers, lamb's-quarters, winter certain herbaceous plants with big flowers and pale persicaria HZ-1 process group；
Fig. 5 be the application Alternaria tenuissima HZ-1 to highland barley, rape, wheat, pea,
The Security test of broad bean, wherein, 1-5 is respectively highland barley, rape, wheat, pea, silkworm
Beans control group；1 '-5 ' it is respectively highland barley, rape, wheat, pea, broad bean HZ-1 process group.
Detailed description of the invention
Presently filed embodiment is described in detail below in conjunction with drawings and Examples, the most right
How application technology means solve technical problem and reach the realization of technology effect the application
Journey can fully understand and implement according to this.
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially
Obtain.
The separation of embodiment 1 bacterium and qualification
One, the isolation and purification of bacterium
With reference to " planting disease research method " (Fang Zhongda writes, Chinese agriculture publishing house, 2007.4)
In about the separation method of disease fungus, to gathering from the geranium wilfordii plant disease leaf helped each other by tissue
Partition method carries out isolated and purified, and rear 4 DEG C of Refrigerator stores are cultivated on inclined-plane.According to locality separating
The Strain Designation obtained is HZ-1.
Two, identify
Bacterial strain HZ-1 is placed on PDA plate culture medium and cultivates, routine observation bacterium colony
The speed of growth, colonial morphology and color and luster change, its mycelia of optical microphotograph Microscopic observation, spore shape
State.In conjunction with " Fungal identification handbook " (Wei Jing overstaffs work, Shanghai science tech publishing house, 1979.9)
Carry out Preliminary Identification.
The form of thalline and physio-biochemical characteristics:
Bacterial strain HZ-1 bacterium colony on PDA plate loosens, easy picking.Bacterium colony is circular, the initial stage
For white, the later stage gradually becomes black in colony edge, until whole flat board.Substrate mycelium and
Aerial hyphae is flourishing, and radially, and substrate mycelium is black in the initial stage to aerial hyphae, micro-
Observe display mycelia have every.Conidium easily sprouts, have every.
(2) 16S rDNA test
Cell Total DNA extraction method reference Wang Yanqin (genomics and applied biology, 2010,
5) method in.
Fungi universal primer ITS 1 and ITS 4, its sequence is as follows:
ITS 1 (5'-TCCGTAGGTGAACCTGCGG-3'), its nucleotide sequence is such as
Shown in SEQ ID NO:1,19bp；
ITS 4 (5'-TCCTCCGCTTATTGATGTGC-3'), its nucleotide sequence is such as
Shown in SEQ ID NO:2,20bp；
Pcr amplification reaction system is as follows: 4 μ L dNTP Mixture, ITS4 and ITS5 are each
10 × Taq reaction buffer of 1.0 μ L, 6.0 μ L, 1.0 μ L Taq DNA polymerase,
Template DNA is 2 μ L, 35.0 μ L aseptic deionized waters.PCR cycle is provided that 94 DEG C
Denaturation 5min；94 DEG C of sex change 1min, 51 DEG C of annealing 1min, 72 DEG C extend 1min, altogether
If 35 circulations.Collect PCR primer to check order, its nucleotide sequence such as SEQ ID NO:3
Shown in, size is 545bp, and sequencing result chromas.exe software carries out sequence correction,
From the DNA sequence dna that NCBI Blast retrieval is high with target sequence homology, surveyed with this test
Sequence carries out comparison in bacterial strain kind respectively.MEGA5.0 software neighbour is utilized to return group method to build system
System grows tree.
Use universal primer ITS1 and ITS4, expand in the genomic DNA of bacterial strain HZ-1
Obtain the ITS fragment that clip size is 545bp.Find ITS and the rod method Pseudomonas of this bacterium
ITS is the most similar, wherein the highest with the homology of Alternaria sp..Combining form feature,
Qualification result shows: bacterial strain HZ-1 and (Alternaria tenuissima) are same genus.
Based on features above, bacterial strain HZ-1 is accredited as Alternaria tenuissima.This bacterium
It is general that strain was preserved in China Committee for Culture Collection of Microorganisms on 01 11st, 2016
Logical microorganism center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, section of China
Institute of microbiology of institute, postcode 100101), preserving number is CGMCC No.11992.
The embodiment 2 bacterial strain HZ-1 pathogenic impact on broad leaved weed
By field growing 4～5 leaf phase, leaf look normal lamb's-quarters, winter certain herbaceous plants with big flowers, pale persicaria, 4～6 take turns
The clearvers of leave phase is transplanted in flowerpot (Φ=15cm), cultivates in laboratory；Will be raw
After anti-fungal expands cultivation 120h in PDB nutrient solution, make 107Individual mL-1Zymotic fluid
Rear spray inoculation to transplant healthy weeds plant on, inoculum concentration is 30mL/ basin.With inoculation
The plant of PDB nutrient solution is as blank；If 3 times are repeated.Postvaccinal weeds plant
It is placed in room temperature, keeps relative humidity to be 60% with JBX-3.5 centrifugal industry humidifier, 12h
Alternation of light and darkness is cultivated.7d " Invest, Then Investigate " weeds occurring degree, claims fresh weight, calculates the incidence of disease, fresh
Heavily effect, disease index, all data acquisitions DPS statistical software carries out significance of difference analysis.
As shown in Figure 3 and Figure 4, after spraying bacterial strain HZ-1 zymotic fluid, 5 kinds of weeds show
Damage symptoms in various degree, clearvers shows as leaf rolling, and cauline leaf turns black, lamb's-quarters and garden sorrel
Wilting occurs in top vane, and cluster mallow leaf sheet chlorisis turns yellow, and large area scab occurs in field thistle.Knot
Close the incidence of disease, fresh weight effect and disease index comprehensively to analyze (being shown in Table 1), HZ-1 zymotic fluid pair
Clearvers, lamb's-quarters causative effect are prominent, and significant difference compared with other broad leaved weed, HZ-1 can
As preventing and kill off the candidate strain of clearvers, lamb's-quarters, continue to research.
Table 1 bacterial strain fermentation liquor is to different weeds pathogenicities
Note: same column difference lowercase represents significant difference in 0.05 level.
The embodiment 3 bacterial strain HZ-1 impact on the security of crop
By main for 5 kinds of Qinghai Province cultivation crop: spring wheat (youth 587), spring rape (blue or green miscellaneous No. 5),
Broad bean (Qinghai 9), pea (grassland 224), highland barley (blue or green No. 8 of north) are planted respectively in Φ=15cm
In flowerpot, indoor cultivation is standby.3～4 it are inoculated in as stated above after the dilution of HZ-1 zymotic fluid
On the crop plant of leaf phase, if 3 times are repeated, after inoculation 7d, observe the morbidity feelings for studying thing
Condition, records occurring degree.
As it is shown in figure 5, to spring wheat (youth 587), pea after the inoculation of HZ-1 bacterial strain fermentation liquor
(grassland 224), broad bean (Qinghai 9), highland barley (blue or green No. 8 of north) all no pathogenicity, crop length
Gesture and plant height are the most uninfluenced compared with adjoining tree, are presented without reacting (NS)；Right
Spring rape (blue or green miscellaneous No. 5) has slight pathogenic, 3d after inoculation, and wilting occurs in 5% blade,
There are fragmentary spot, later stage symptom not to extend, show as minor response (LS).
Described above illustrate and describes some preferred embodiments of invention, but as it was previously stated, should
When understanding that invention is not limited to form disclosed herein, it is not to be taken as other embodiments
Eliminating, and can be used for other combinations various, amendment and environment, and can be at described herein
In bright contemplated scope, it is modified by above-mentioned teaching or the technology of association area or knowledge.And
Change that those skilled in the art are carried out and change, the most all should be without departing from the spirit and scope of invention
In the protection domain of invention claims.
Claims (8)
1. a strain Alternaria tenuissima, it is characterised in that its Classification And Nomenclature is Alternaria tenuissima,
Alternaria tenuissima, this bacterial strain was preserved in Chaoyang District, city of BeiJing, China on 01 11st, 2016
No. 3 China Committee for Culture Collection of Microorganisms's common micro-organisms centers of North Star West Road 1 institute, preservation
Number it is CGMCC No.11992.
2. a preparation method for the ferment filtrate of Alternaria tenuissima as claimed in claim 1, it is special
Levy and be, comprise the following steps: the colony edge after cultivating 7d takes mycelia block and breaks into a diameter of 5mm
Bacterium cake, is inoculated in 200mLPDB nutrient solution, every bottle graft 5 pieces, 25 DEG C of 180r min-1Cultivate 5d,
Nutrient solution vacuum filtration, filtrate with 0.45 μm filtering with microporous membrane, obtains ferment filtrate again.
3. the application in biological weed control of the Alternaria tenuissima described in claim 1.
Application the most according to claim 3, it is characterised in that the ferment filtrate of this bacterial strain is sprayed
On highland barley, broad bean or pea, plant performance safety.
Application the most according to claim 4, it is characterised in that described ferment filtrate particular by
Following methods prepares: the colony edge after cultivating 7d takes mycelia block and breaks into a diameter of 5mm bacterium cake,
It is inoculated in 200mLPDB nutrient solution, every bottle graft 5 pieces, 25 DEG C of 180r min-1Cultivate 5d, nutrient solution
Vacuum filtration, filtrate with 0.45 μm filtering with microporous membrane, obtains ferment filtrate again.
Application the most according to claim 4, it is characterised in that the amount of spraying of described ferment filtrate is
50mL/m2。
7. require the application described in any claim in 3 to 6, its feature according to claims
Being, described grass is broad leaved weeds.
Application the most according to claim 7, it is characterised in that described broad leaved weeds be clearvers,
One or several in cottonsedge-like thistle herb, lamb's-quarters, winter certain herbaceous plants with big flowers or pale persicaria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610229836.8A CN105861318A (en) | 2016-04-13 | 2016-04-13 | Alterneria tenuissima and application thereof in biological weeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610229836.8A CN105861318A (en) | 2016-04-13 | 2016-04-13 | Alterneria tenuissima and application thereof in biological weeding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105861318A true CN105861318A (en) | 2016-08-17 |
Family
ID=56637780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610229836.8A Pending CN105861318A (en) | 2016-04-13 | 2016-04-13 | Alterneria tenuissima and application thereof in biological weeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105861318A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635827A (en) * | 2016-11-07 | 2017-05-10 | 青海省农林科学院 | Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding |
| CN107969423A (en) * | 2017-11-22 | 2018-05-01 | 青海省农林科学院 | A kind of toVerticilliumdahliaActivitie Activitie S of Relative bacterial strain wettable powder and preparation method thereof |
| CN107988084A (en) * | 2017-11-22 | 2018-05-04 | 青海省农林科学院 | A kind of fermentation process of Alternaria tenuissima |
| CN114032179A (en) * | 2021-09-27 | 2022-02-11 | 黑龙江省农业科学院经济作物研究所 | CBD-producing industrial hemp endophytic fungus and application thereof |
| NL2035080B1 (en) * | 2023-05-06 | 2024-01-23 | Qinghai Acad Of Agricultural And Forestry Sciences | Alternaria gaisen and its application in biological weeding |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293241A (en) * | 2000-09-20 | 2001-05-02 | 南京农业大学 | Method for using crude metabolite of alternaric bacteria in biologic herbiciding |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
-
2016
- 2016-04-13 CN CN201610229836.8A patent/CN105861318A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293241A (en) * | 2000-09-20 | 2001-05-02 | 南京农业大学 | Method for using crude metabolite of alternaric bacteria in biologic herbiciding |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
Non-Patent Citations (6)
| Title |
|---|
| MÜLLER M.: "Toxin-producing ability of molds of the genus Alternaria", 《ZENTRALBL MIKROBIOL.》 * |
| N. D. DAVIS等: "Tenuazonic Acid Production by Alternaria alternata and Alternaria tenuissima Isolated from Cotton", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
| STEFANIA SOMMA等: "AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree", 《INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY》 * |
| 曾秘 等: "微生物除草剂的研究现状", 《江西农业学报》 * |
| 李永龙: "杂草生防菌的筛选及对阔叶杂草致病性的测定", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 韩丽霞: "1-[1-(吡咯啉-2-酮-3-基)亚乙基]-4-烃基氨基硫脲类衍生物的合成与生物活性研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635827A (en) * | 2016-11-07 | 2017-05-10 | 青海省农林科学院 | Trichoderma polysporum fungus strain HZ-31 and application thereof to biological weeding |
| CN107969423A (en) * | 2017-11-22 | 2018-05-01 | 青海省农林科学院 | A kind of toVerticilliumdahliaActivitie Activitie S of Relative bacterial strain wettable powder and preparation method thereof |
| CN107988084A (en) * | 2017-11-22 | 2018-05-04 | 青海省农林科学院 | A kind of fermentation process of Alternaria tenuissima |
| CN114032179A (en) * | 2021-09-27 | 2022-02-11 | 黑龙江省农业科学院经济作物研究所 | CBD-producing industrial hemp endophytic fungus and application thereof |
| CN114032179B (en) * | 2021-09-27 | 2023-06-09 | 黑龙江省农业科学院经济作物研究所 | Industrial cannabis endophytic fungus for producing CBD and application thereof |
| NL2035080B1 (en) * | 2023-05-06 | 2024-01-23 | Qinghai Acad Of Agricultural And Forestry Sciences | Alternaria gaisen and its application in biological weeding |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104745483B (en) | A kind of Paecilonyces variotii strain SJ1 and its application | |
| CN105861318A (en) | Alterneria tenuissima and application thereof in biological weeding | |
| CN104560827B (en) | A kind of biocontrol actinomycetes bacterial strain for preventing and treating tobacco bacterial wilt and its application | |
| CN107142229B (en) | It is a kind of prevent and treat capsicum epidemic disease biocontrol actinomycetes bacterial strain and its application | |
| CN104195050B (en) | The one the narrowest Lecanicillium lecanii of strain and the application in preventing and treating crop nematode and aleyrodid thereof | |
| CN107058160A (en) | One plant of peanut rhizosphere bacillus amyloliquefaciens and its application | |
| CN105647829A (en) | Bacillus amyloliquefaciens and application thereof to prevention and treatment of sugarcane red rot | |
| CN105441331B (en) | One plant of myrothecium roidium and its application | |
| CN104450560B (en) | One plant of nematicide Sphingobacterium bacterial strain and its application | |
| CN105779360A (en) | Bacillus subtilis and application thereof | |
| CN106010987B (en) | A kind of muscardine bacterial strain and its application to prominent back sugarcane rhinoceros cockchafer with pathogenicity | |
| CN105039168A (en) | Trichoderma citrinoviride and application thereof | |
| CN105062897B (en) | The Trichoderma viride of one plant height production chlamydospore and its application | |
| CN106399131B (en) | One plant of production dark purple mould and its application | |
| CN111778190A (en) | Brevibacillus brevis for preventing and treating root knot nematode disease and application thereof | |
| CN107325980A (en) | A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant | |
| CN103667083B (en) | A kind of mould, its cultural method of top spore and the application in preparation original spore powder of meloidogyne pesticide thereof | |
| CN102559509B (en) | Screening and application of Bohai Bay acremonium cephalosporium | |
| CN108441443B (en) | Strain for preventing and treating plant nematodes and application thereof | |
| CN110669675A (en) | Metarhizium anisopliae MANGS71814 and application thereof in control of potato tuber moth | |
| CN102925369B (en) | Chaetomium globosum with nematicidal activity, metabolite and application thereof | |
| CN109735457A (en) | One plant of mutagenesis Infected barnyardgrass and its application in prevention and treatment barnyard grass | |
| CN103642700B (en) | One strain aureobasidium pullulans and application thereof | |
| CN102229892B (en) | Nomuraea rileyi strain, and application thereof | |
| Cheng et al. | Biological control of Qinghai plateau terrestrial weeds with the A. alternata HL-1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160817 |