CN1293241A - Method for using crude metabolite of alternaric bacteria in biologic herbiciding - Google Patents
Method for using crude metabolite of alternaric bacteria in biologic herbiciding Download PDFInfo
- Publication number
- CN1293241A CN1293241A CN 00112560 CN00112560A CN1293241A CN 1293241 A CN1293241 A CN 1293241A CN 00112560 CN00112560 CN 00112560 CN 00112560 A CN00112560 A CN 00112560A CN 1293241 A CN1293241 A CN 1293241A
- Authority
- CN
- China
- Prior art keywords
- alternaric bacteria
- agent
- bacteria
- weeds
- weed control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
A method for biological herbiciding with the crude metabolite of alternaric bacteria includes such steps as collecting the raw liquid mixture of said crude metabolite, diluting with water by 10-15 times and spraying it onto weeds. The used culture medium may be liquor one of corn and soybean powder, or potato and cane sugar, or solid one of wheat grains. Its advantages are broad spectrum (latifolious weeds, grass and nutgrass flatsedge) and quickly taking its high effect (5-48 hr).
Description
The present invention is the method that the crude metabolite of alternaric bacteria is used for biological weed control, belongs to the technical field that microorganism is applied to agriculture plant protection, prevents and kill off crop weeds, is exclusively used in preventing and kill off of farmland weed.
It is to separate the plant poison that obtains is administered weeds as weedicide a kind of method with the meta-bolites of microorganism or from microorganism that microbe metabolite is controlled grass.The emphasis or the focus of research and development have now been become.This class weedicide is all more superior than living microorganism aspect the consistency of storage, application, preparation and transformation period, and non-target plant is caught an illness, and its drug effect does not rely on environmental factors usually yet, is convenient to prediction.Fennel toxin (Anisomycin) is the metabolic product of streptomycete, weeds such as energy strongly inhibited barnyard grass grass and lady's-grass." nuisanceless " weedicide methoxy benzophenone is exactly according to the imitated a kind of herbicide for paddy field that forms of Anisomycin, and paddy rice and barnyard grass grass are had the selectivity of height, suppresses barnyard grass grass chlorophyll and forms, and produces albefaction and causes death.11 kinds in the Penicillium (Penicillium frequentans) can produce N-formyl radical amidoxyl acetic acid after fermentation, can kill lady's-grass and some broadleaf weeds.The tunning phenazine-1-carboxylic acid and the 2 hydroxyl phenazine-1-carboxylic acids of golden false pseudomonas bacillus (Psendomonasaurecafaciens), 10 pounds every acre dosage can be prevented and kill off Herba Setariae Viridis, watercress and waterplant blue-green algae and duckweed (Lemna sp.) etc.
This is wherein foremost will count bialaphos, and it is the product by streptomyces hygroscopicus (Streptomyces hygruscopicus) fermentation, in Japan's application widely.The steriland herbicide glufosinates of developing according to the chemical structure of bialaphos (careless fourth phosphine) is in American-European countries's widespread use.And, utilize transgenic technology to develop anti-glufosinates crop, extensively cultivated, further, enlarged the use range of this weedicide.
Utilize the spore alive of alternaric bacteria to make campelyco, the existing a lot of reports of management of weeds.But, utilize meta-bolites to carry out weeding, only having with AAL-toxin toxin and alternaric acid is the report that main effective constituent is carried out weeding.Patent has been applied in the grass effect of killing of AAL-toxin toxin.In this patent, once mentioned the weeding activity of the crude extract that contains this toxin, it mainly contains these features and is: be primarily aimed at some broadleaf weeds as: black nightshade, stramonium, Virginia close sprout, big fruit sesbania etc., to the gramineous weeds nearly unavailable; Crude extract is after carrying out solid culture with solid medium such as Semen Maydis powder or rice, the filtered liquid that obtains through washing filtering, the active concentration of tool at 1000ppm with first-class.Alternaric acid also has similarly only to the effective characteristics of broadleaved herb.But the kind of these two kinds of toxin weedings is less, can not prevent and kill off dogstail and nutgrass flatsedge, and the kind of preventing and kill off of broadleaved herb is also lacked; Solid culture throughput is relatively low, is unsuitable for large-scale industrial production.
China does not have a kind of campelyco industrialization at present as yet, objectively advance the industrialization of campelyco, to change the product structure of the present weedicide of China, the industrial structure of pesticide chemical, and satisfy green and Organic food production demand to nuisanceless weedicide.
The objective of the invention is to prevent and kill off dogstail and nutgrass flatsedge, the kind of preventing and kill off of broadleaved herb is also lacked at existing alternaric bacteria toxin; Solid culture throughput is relatively low, be unsuitable for the defective of large-scale industrial production, provide a kind of crude metabolite of alternaric bacteria to be used for the method for biological weed control, directly utilize and contain the thick meta-bolites of alternaric bacteria of plant poison activeconstituents as the biogenic chemical herbicide, be used for biological weed control, as the broad spectrum weeding agent, the cauline leaf spraying is handled, and controls gramineous weeds, broadleaved herb and nutgrass flatsedge safely and effectively.Be suitable for the culture technique of large-scale industrial production.Environmental pollution is little, broad weed-killing spectrum, rapidly careless extremely.
The crude metabolite of alternaric bacteria of the present invention is used for the method for biological weed control, and its content and embodiment are as follows:
The thick meta-bolites of alternaric bacteria is used to control method for weed: the microbial strain culture fritter that directly utilizes alternaric bacteria (Alternariaalternata (Fr.) Keissler) to cultivate through PDA, adopt liquid culture or wheat solid medium to cultivate, after filtering out viable bacteria silk and other active cells, the mixture stoste of its thick meta-bolites, through 15 times of water dilutions with interior between concentrating 10 times, wherein the activity of crude metabolite is below 1000 μ g/ml.Spray on the weeds cauline leaf, it is dead rapidly to cause weeds in 48 hours, and these weeds have comprised main broadleaf weeds, dogstail and nutgrass flatsedge.
Liquid cultivating method is: liquid nutrient mediums such as corn and analysis for soybean powder liquid nutrient medium (SCSC), potato sucrose liquid nutrient medium (PSK), Hemp Eupatorium leaf juice substratum a kind of:
PSK substratum: 200g potato poach filtered liquid (four layers of filtered through gauze), 30g sucrose, 1gK
2HPO
4, add water to 1L.
SCSC substratum: 15g Semen Maydis powder+15g analysis for soybean powder, 30g sucrose, 3gCaCO
3, add water to 1L.
Hemp Eupatorium leaf juice substratum: 200g Hemp Eupatorium leaf poach filtered liquid, 30g sucrose, 1gK
2HPO
4, add water to 1L.
Per 1 liter of nutrient solution places 3 liters of left and right sides volumetrical culturing bottles, and under 23-26 ℃ of temperature, dark or 12 hours illumination/12 hour dark replacing are left standstill or shake (110 rev/mins) and cultivated 5-8 days.With the nutrient solution filter paper filtering, filtrate is got supernatant liquor with 0.45 μ m microporous membrane filtration through high speed centrifugation, gets bacteria-free filtrate.
The wheat groat solid medium takes by weighing 100g, in the triangular flask of the 500ml that packs into, with clear water wash in a pan clean after, soak 10h again, remove excessive moisture, place 30 minutes (121 ℃) of Autoclave sterilization, the strain of cooling back inoculation alternaric bacteria is cultivated under 25 ℃, the condition of 12hL (light)/12hD (secretly), shakes triangular flask every day 1-2 time, avoid caking, 3 weeks back taking-up culture, 80 ℃ of oven dry down, pulverize, use the ethyl acetate extracting then 3 times, ethyl acetate is reclaimed in air distillation, gets thick toxin.
Bacteria-free filtrate can add the auxiliary agent of 1-5% with interior between concentrating 10 times through diluting 15, and auxiliary agent is nonionic surface active agent, plant-sourced auxiliary agent and permeate agent.Main kind has synergistic agent SD (SD), Sodium dodecylbenzene sulfonate (12WB), farming newborn No. 100 (NR100) and 0.01-0.5% permeate agent etc.Sparge on the weeds seedling of 2-5 leaf phase, through more than 5 hours, the weeds seedling symptom that here occurs withering is again through 2 days left and right sides time and can kill weeds fully.Can kill main broadleaf weeds, dogstail and the nutgrass flatsedges in farmland such as lady's-grass, Herba Eleusines Indicae, bare headed barnyard grass, barnyard grass grass, wild avena sativa, amur foxtail, Japanese amur foxtail, Wang grass, hard grass, Herba Eleocharitis acicularis, Eclipta prostrata, Herba Acalyphae, Tender Catchweed Bedstraw Herb, Herba Viviae Sativae, Herb of Common Nipplewort, lamb's-quarters, ox chickweed, Stellaria alsine Grim., mexicana, Sheathed Monochoria, descurainia sophia (l.) webb ex prantl, Tradescantia albiflora, water peanut, Amaranthus retroflexus, wild amaranth, shepherd's purse.
The contriver has found the natural occurrence phenomenon of open-air Hemp Eupatorium in the biological control research process to Hemp Eupatorium, so its parasitical fungi is carried out screening study.Separate to have obtained alternaric bacteria (Alternaria alternata (Fr.) Keissler) bacterial strain, its biological characteristics, pathogenic and pathogenesis have been carried out deep research, find the generation that its morbific main mechanism of action is the pathophyte toxin.By to the cultivation raffinate of alternaric bacteria behind filtering viable bacteria after the liquid culture, the thick toxin of test cultures raffinate, extraction and the weeding biological activity of separating the toxin of purifying, discovery just can make weeds produce tangible poisoning symptom at 1-1000 μ g/ml with interior, the fastest time that causes poisoning symptom is at 5 hours, can kill weeds fully in 2 days.Discover to for the examination 94 kind of plant in, only have 3 kinds insensitive.
Slightly the preparation of toxin is after bacterial strain is cultivated by above-mentioned condition, nutrient solution with 4 layers of filtered through gauze, to be used common filter paper suction filtration again, gets filtrate and uses equal-volume ethyl acetate extraction 3 times, and the combined ethyl acetate phase reclaims the ethyl acetate distillation, gets thick toxin.Thick toxin is diluted with water to specific concentrations again through a small amount of raw spirit dissolving, handles weeds.
The position that the alternaric bacteria toxin soiutions is handled obviously is injured, and the fastest time of appearance of symptom caused 48 hours at 5 hours, and the weeds position that is subject to processing is downright bad fully.The toxic action of toxin, the position that only limits to be subjected to chemicals treatment, almost not conduction.
The alternaric bacteria toxin influences the saturating property of weeds excised leaf cytolemma, and along with the increase of toxin concentration, the relative conductivity of leach liquor rises.When toxin concentration when 1 is increased to 2.5 μ g/g, the relative conductivity of leach liquor changes very little, and when toxin concentration when 2.5 are increased to 5 μ g/g, the relative conductivity of leach liquor alters a great deal, and increases more than 2 times, when toxin concentration increases again, the relative conductivity of leach liquor rises very little, and this result shows, the absorption of toxin may with film on receptor related, when acceptor by after saturated, saturating relatively property no longer is subjected to the influence of toxin concentration.The alternaric bacteria toxin can cause the K of Hemp Eupatorium leaf texture
+And Na
+Seepage, along with the increase of toxin concentration, K in the leach liquor
+And Na
+Content rise.
Cause that it is the reaction that nosotoxin acts on the ubiquity that takes place behind the host that cell leakage changes.The nosotoxin that bacterial strain produces can cause the saturating property rising of weeds excised leaf cytolemma, K
+, Na
+Seepage, the film fat peroxidation of leaf texture aggravation illustrates that nosotoxin causes the injury of cytolemma, causes the disorder of film function.After nosotoxin is handled, the activity of the peroxidase of weeds excised leaf (POD), ascorbate peroxidase enzyme (APX), catalase (CAT) descends, enzyme system is destroyed, the active oxygen excess accumulation, therefore cell comes to harm, and finally makes grass cutting blade show the symptom of being injured.
The present invention compared with prior art, its major advantage and positively effect are as follows:
1) alternaric bacteria adopts corn or rice solid medium to cultivate in the prior art, be primarily aimed at some broadleaf weeds as: black nightshade, stramonium, Virginia close sprout, big fruit sesbania etc., to the gramineous weeds nearly unavailable; Alternaric bacteria of the present invention utilizes the method for liquid fermentation and culture through the thick meta-bolites with liquid culture, is convenient to mass industrialized production.Also can adopt wheat solid medium solid culture, produce thick toxin, all can be used to control the main broadleaf weeds in farmland, dogstail and nutgrass flatsedge.The direct utilization of thick toxin, the extraction of saving effective constituent with separate, simplify technical process, reduce the investment of equipment, reduce production costs.
2) alternaric bacteria product weeding in the prior art will be more than 2 days.Among the present invention, alternaric bacteria is through with the crude extract of liquid culture or its effective constituent control weeds, and it is fast to kill careless speed, can in 5 hours weeds is injured, and about 2 days weeds is killed fully.When this changes crops to the farmland or sowing pre-treatment weeds tool have an enormous advantage.It kills grass based on contact killing type, also is convenient in use utilize the potential difference selectivity.Its mode of action is similar to the chemical herbicide Paraquat that generally uses at present.But the crude extract toxicity of alternaric bacteria liquid culture significantly is lower than Paraquat, and activity is also higher.
3) crude extract of alternaric bacteria liquid culture or its effective constituent is active higher, and the former effective concentration is below 1000ppm, and the latter's effective concentration more is low to moderate 1-25ppm.Because active high, consumption reduces, the contaminative of farm environment is reduced relatively.
4) crude extract of alternaric bacteria liquid culture or its effective constituent, directly spray on the stem of weeds, being used for cauline leaf handles, can control most main farmland weeds, broadleaf weeds, dogstail and nutgrass flatsedges such as lady's-grass, Herba Eleusines Indicae, bare headed barnyard grass, barnyard grass grass, wild avena sativa, amur foxtail, Japanese amur foxtail, Wang grass, hard grass, Herba Eleocharitis acicularis, Eclipta prostrata, Herba Acalyphae, Tender Catchweed Bedstraw Herb, Herba Viviae Sativae, Herb of Common Nipplewort, lamb's-quarters, ox chickweed, Stellaria alsine Grim., mexicana, Sheathed Monochoria, descurainia sophia (l.) webb ex prantl, Tradescantia albiflora, water peanut, field thistle, Amaranthus retroflexus, wild amaranth, shepherd's purse.Thereby the grass spectrum is wide especially extremely, and range of application will be more extensive.Other sees Table 1.
The different floristics of table 1 are to the susceptibility of activeconstituents
(plant is to the susceptibility grade scale of thick toxin: 0 grade, insensitive: 1 grade, responsive slightly; 2 grades, responsive; 3 grades, sensitivity; 4 grades, very sensitive; 5 grades, extremely responsive)
Embodiment: embodiment 1: respectively in Australian Sydney, gather on the Hemp Eupatorium disease plant of the open-air self-sows such as Yunnan Province of Wollongong and China and be separated to 6 bacterial strains, warp is to 501,402,503, LS, YN, 6 bacterial strain bacterial classifications such as AW on the PDA substratum, cultivate 7 days (25 ℃, L: D=12: 12), cut-off directly is the mycelia piece of 6.5mm size then, be inoculated into (500ml triangular flask) in the 300mlPSK liquid nutrient medium, cultivate 5-7 days (25 ℃, L (light): D (secretly)=12: 12,110 rev/mins), begin blackening to mycelia, with the nutrient solution filter paper filtering, filtrate is through high speed centrifugation, get supernatant liquor with 0.45 μ m microporous membrane filtration, get bacteria-free filtrate.Prepare bacteria-free filtrate with filtrate, be inoculated on the Hemp Eupatorium excised leaf with needle punching, after in culture dish, cultivating (25 ℃, 12hL (light)/12hD (secretly), filter paper are preserved moisture) interval certain hour, place the diameter of observing and write down scab under the anatomical lens, last statistical study determines that bacterial strain produces the difference of malicious ability.The PSK (CK2) of sterilized water (CK1) and aseptically process is adopted in contrast respectively.
Test-results shows: bacterial strain 501 culture bacteria-free filtrates are the strongest to the virulence of Hemp Eupatorium, thereby nosotoxin content wherein should be the highest, so the product poison ability of this bacterial strain is apparently higher than other each bacterial strain.Thereby, illustrate that also the product poison ability between the different strains of alternaric bacteria is discrepant.Embodiment 2: in the SCSC liquid nutrient medium, is that 25 ± 1 ℃, illumination be 12h/d, shake speed for the condition of 110rpm/min under cultivate 5-7 days in temperature with 501 inoculation.With the nutrient solution filter paper filtering, filtrate is got supernatant liquor with 0.45 μ m microporous membrane filtration through high speed centrifugation, gets bacteria-free filtrate.
With filtered liquid is concentrated the lady's-grass seedling that 20 times, 10 times, 5 times, 2.5 times, 1 times, stoste or the eupatorium toxin concentrated solution through diluting 1 times, 2.5 times, 5 times, 10 times, 20 times are sprayed to tri-leaf period respectively through heating.After handling 1 day, 2 days, 3 days, 7 days, observe the weeds victimization state respectively.Table 2 alternaric bacteria toxin different concns concentrated solution is to the preventive effect of lady's-grass seedling in tri-leaf period
N: filter stoste and concentrate X: data are for preventing and kill off percentage in the filtration stoste dilution table.
The different floristics of table 3 are to the susceptibility of the thick toxin of 501 bacterial strains
(plant is to the susceptibility grade scale of thick toxin: disease index (%) 0-0 level, and insensitive; The 1-20-1 level, responsive slightly; The 21-40-2 level, responsive; The 41-60-3 level, sensitivity; The 61-80-4 level, very sensitive; The 81-100-5 level, extremely responsive) embodiment 3: add as production alternaric bacteria crude extract as described in the example 2, add auxiliary agent and 0.1% permeate agent, can obviously improve alternaric bacteria crude extract herbicidal effect.The different auxiliary agents of table 4 kill the influence (is lady's-grass for the examination weeds) of grass effect to the alternaric bacteria crude extract
X: data are for preventing and kill off percentage in the filtration stoste dilution table.
Claims (5)
1. the crude metabolite of lattice spore bacterium is used for the method for biological weed control, it is characterized in that: the microbial strain culture fritter that directly utilizes alternaric bacteria (Alternaria alternata (Fr.) Keissler) to cultivate through PDA, adopt liquid culture or wheat solid medium to cultivate, after filtering out viable bacteria silk and other active cells, the mixture stoste of its thick meta-bolites, through 15 times of water dilutions with interior between concentrating 10 times, spray on the weeds cauline leaf.
2. alternaric bacteria crude metabolite according to claim 1 is used for the method for biological weed control, and wherein liquid nutrient medium is: corn and analysis for soybean powder liquid nutrient medium (SCSC), potato sucrose liquid nutrient medium (PSK), wheat groat solid medium etc.
3. alternaric bacteria crude metabolite according to claim 1 and 2 is used for the method for biological weed control, it is characterized in that: bacteria-free filtrate through dilute 15 times with interior between concentrating 10 times, the auxiliary agent that can add 1-5%, auxiliary agent are nonionic surface active agent, plant-sourced auxiliary agent and permeate agent.
4. alternaric bacteria crude metabolite according to claim 1 and 2 is used for the method for biological weed control, it is characterized in that: auxiliary agent is synergistic agent SD, Sodium dodecylbenzene sulfonate, No. 100, farming breast and 0.01-0.5% permeate agent etc.
5. alternaric bacteria crude metabolite according to claim 3 is used for the method for biological weed control, it is characterized in that: auxiliary agent is synergistic agent SD, Sodium dodecylbenzene sulfonate, No. 100, farming breast and 0.01-0.5% permeate agent etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00112560 CN1128210C (en) | 2000-09-20 | 2000-09-20 | Method for using crude metabolite of alternaric bacteria in biologic herbiciding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00112560 CN1128210C (en) | 2000-09-20 | 2000-09-20 | Method for using crude metabolite of alternaric bacteria in biologic herbiciding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1293241A true CN1293241A (en) | 2001-05-02 |
| CN1128210C CN1128210C (en) | 2003-11-19 |
Family
ID=4582413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00112560 Expired - Fee Related CN1128210C (en) | 2000-09-20 | 2000-09-20 | Method for using crude metabolite of alternaric bacteria in biologic herbiciding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1128210C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006079275A1 (en) * | 2005-01-28 | 2006-08-03 | Nanjing Agricultural University | Biological control of weeds using the metabolites of alternaria alternata |
| CN102191184A (en) * | 2011-04-08 | 2011-09-21 | 中国计量学院 | Biocontrol endophytic fungi-Alternaria alternata |
| CN102329736A (en) * | 2011-08-30 | 2012-01-25 | 东华大学 | Alternaria alternata DB2 strain and preparation and application thereof |
| CN103088138A (en) * | 2013-01-25 | 2013-05-08 | 河南农业大学 | Detection primer and molecular detection method of wheat black point dominant pathogen alternaria |
| CN103404536A (en) * | 2013-08-21 | 2013-11-27 | 南京农业大学 | Compound biological herbicide and application method thereof |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
| CN105861318A (en) * | 2016-04-13 | 2016-08-17 | 青海省农林科学院 | Alterneria tenuissima and application thereof in biological weeding |
| CN114806896A (en) * | 2022-06-06 | 2022-07-29 | 青岛农业大学 | Alternaria alternata, herbicide and application thereof |
| CN116904327A (en) * | 2023-09-13 | 2023-10-20 | 中国农业科学院草原研究所 | Alternaria alternata capable of preventing and killing Tribulus terrestris, microbial inoculum and application thereof |
-
2000
- 2000-09-20 CN CN 00112560 patent/CN1128210C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006079275A1 (en) * | 2005-01-28 | 2006-08-03 | Nanjing Agricultural University | Biological control of weeds using the metabolites of alternaria alternata |
| CN102191184A (en) * | 2011-04-08 | 2011-09-21 | 中国计量学院 | Biocontrol endophytic fungi-Alternaria alternata |
| CN102329736A (en) * | 2011-08-30 | 2012-01-25 | 东华大学 | Alternaria alternata DB2 strain and preparation and application thereof |
| CN102329736B (en) * | 2011-08-30 | 2013-01-16 | 东华大学 | Alternaria alternata DB2 strain and preparation and application thereof |
| CN103088138B (en) * | 2013-01-25 | 2014-02-12 | 河南农业大学 | Detection primer and molecular detection method of wheat black point dominant pathogen alternaria |
| CN103088138A (en) * | 2013-01-25 | 2013-05-08 | 河南农业大学 | Detection primer and molecular detection method of wheat black point dominant pathogen alternaria |
| CN103404536A (en) * | 2013-08-21 | 2013-11-27 | 南京农业大学 | Compound biological herbicide and application method thereof |
| CN103404536B (en) * | 2013-08-21 | 2015-11-25 | 南京农业大学 | A kind of compound biological herbicide and using method thereof |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
| CN105861318A (en) * | 2016-04-13 | 2016-08-17 | 青海省农林科学院 | Alterneria tenuissima and application thereof in biological weeding |
| CN114806896A (en) * | 2022-06-06 | 2022-07-29 | 青岛农业大学 | Alternaria alternata, herbicide and application thereof |
| CN116904327A (en) * | 2023-09-13 | 2023-10-20 | 中国农业科学院草原研究所 | Alternaria alternata capable of preventing and killing Tribulus terrestris, microbial inoculum and application thereof |
| CN116904327B (en) * | 2023-09-13 | 2023-11-14 | 中国农业科学院草原研究所 | Alternaria alternata capable of preventing and killing Tribulus terrestris, microbial inoculum and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1128210C (en) | 2003-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nagrare et al. | Compendium of cotton mealybugs | |
| Auld et al. | Possible control of Xanthium spinosum by a fungus | |
| CN101445785B (en) | Aspergillus niger fungus capable of poisoning plant parasitic nematodes, preparation method and application thereof | |
| CN105705629A (en) | Isolated strain of clonostachys rosea for use as a biological control agent | |
| US20110136758A1 (en) | Carbohydrate compositions from basidiomycete fungi as biocidal agents active against pathogens | |
| CN101011070A (en) | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice | |
| CN102199558A (en) | Culture method and applications of Pseudomonas aurantiaca | |
| CN1994092A (en) | A biological weed killer and preparation process thereof | |
| CN104130958A (en) | Bacillus subtilis and application of bacillus subtilis in preventing and curing peony root-knot nematode | |
| CN103404536B (en) | A kind of compound biological herbicide and using method thereof | |
| CN102021122A (en) | High-efficiency insecticidal fungus and applications thereof | |
| CN106942279A (en) | A kind of apple tree canker biocontrol agent and its application | |
| CN101182468B (en) | Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof | |
| CN102657235B (en) | Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof | |
| CN1293241A (en) | Method for using crude metabolite of alternaric bacteria in biologic herbiciding | |
| CN100593567C (en) | Culture process of yard grass Bipolaris sacchari and its use | |
| CN106906171A (en) | A kind of preparation method of apple tree canker biocontrol agent | |
| CN103880563A (en) | Culture medium as well as preparation method and application thereof | |
| Deepak et al. | Induction of resistance against downy mildew pathogen in pearl millet by a synthetic jasmonate analogon | |
| CN102925369B (en) | Chaetomium globosum with nematicidal activity, metabolite and application thereof | |
| Deka et al. | Biocontrol prospects of entomopathogenic fungi for management of mustard aphid (Lipaphis erysimi Kalt.) on rapeseed-mustard | |
| CN100489083C (en) | Penicillium fungus with nematocidal activity and its preparing method and use | |
| CN109122727A (en) | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide | |
| CN1439270A (en) | Preparing method for lilacinin against nematoda eggs | |
| CN101555456A (en) | Fusarium philoceroides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20031119 Termination date: 20190920 |