CN104877950B - The bacillus pumilus of one high-efficiency degradation Atrazine - Google Patents
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Abstract
The bacillus pumilus of one high-efficiency degradation Atrazine, it is related to the bacillus of a high-efficiency degradation Atrazine.It is bacillus pumilus (Bacillus pumilus) gy201401, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date is on December 29th, 2014, and preserving number is CGMCC No.10252.It is a kind of very strong microorganism of resistance, using the degraded that Atrazine is remained in significantly accelerated soil after this microorganism, harm of the Atrazine residual to lower stubble sensitive crop is alleviated or avoided, so as to improve the yield of crop, obtains higher economic benefit.
Description
Technical field
The invention belongs to technical field of bioengineering, more particularly to a kind of bud that can repair atrazine-contaminated soil
The discovery of spore bacillus.
Background technology
Atrazine belongs to triazine herbicide, is a kind of selective inner sucting conduction type before seedling, herbicide after seedling, closely
Widely used over year in China, but it belongs to release weedicide, and the residence time is longer, and easily ecological environment is polluted, and its
Residual has serious poisoning to late stubble sensitive crop, is that a major issue is obtained in agricultural production and ecological environment.China is near
Atrazine repeatedly occurs year to late stubble sensitive crop (beet, muskmelon, wheat, soybean etc.) hazardous events, directly contributes serious
Economic loss.
Microorganism is the stronger biological group of degradable organic pollutant ability.Since nineteen eighty-two, scholars are successively thin
The microorganism of the isolated Atrazine that can degrade in bacterium, fungi and algae, and research is more detailed, is ground in terms of actinomyces
That studies carefully is relatively fewer.The main bacteria for the Atrazine that can degrade reported at present has pseudomonas (Pseudomonas
Sp.), alcaligenes (Alcaligens sp.), Agrobacterium (Agrobacterium sp.), acinetobacter
(Acinetabacter sp.), Arthrobacter (Arthrobacter sp.), Bacillus cercus (Bacillus cereus)
Deng.The main fungal of Atrazine of can degrading has microassembly robot (Pencillium janthinellum), red vertical mushroom
(Hypholoma fasciculare), aspergillus fumigatus (Aspergillus fumigatus), rhizopus stolonifer (Rhizopus
Stolonifer), yellow third aspergillus (Aspergillus flavipes), penicillium decumbens (Penicillium decumbens) etc..
As people go deep into microbial degradation Atrazine research, some problems also progressively show, such as in laboratory
In, microorganism is fine to the degradation effect of Atrazine, but is but fallen flat into environment, or even is imitated with laboratory
Fruit is opposite；Toxicity problem of the secondary metabolite of microbial degradation etc..Herein for the farmland pollution problem of Atrazine residual, grind
Study carefully its microbial degradation method, degradation bacteria strains separated using shaking flask acclimating method, using form, Physiology and biochemistry and
Molecular method carries out classification position identification to the bacterial strain filtered out, specifies the growth characteristics and nutritive peculiarity of degradation bacteria strains, simultaneously
And the degradation effect of degradation bacteria strains is evaluated using pot-culture method (access expansion environment to greatest extent).This research is being degraded
Atrazine in soil, mitigate its harm of the residual to second stubble crop, ensure crop production safety, mitigate ecological environmental pollution
Etc. be respectively provided with important meaning.
The content of the invention
The invention provides the bacillus of a high-efficiency degradation Atrazine, and it is to filter out to degrade from soil
Atrazine in soil, the bacterial strain of harm of the Atrazine residual to lower stubble sensitive crop is alleviated or avoided, accelerates so as to reach
The degraded of the Atrazine of pedo relict, it is ensured that crop normal growth, ensure crop production safety, mitigate ecological environmental pollution
The purpose of.
The bacillus pumilus of the high-efficiency degradation Atrazine of the present invention, it is bacillus pumilus (Bacillus
Pumilus) gy201401, is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation address is
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date are on December 29th, 2014, and preserving number is CGMCC No.10252.
Bacillus pumilus (Bacillus pumilus) the gy201401 bacterial strains physiological and biochemical index such as institute of table 1 of the present invention
Show.
The physiological and biochemical index of table 1
Molecular Identification：Bacillus pumilus (Bacillus pumilus) gy201401 bacterial strains of the present invention are carried out
16SrDNA identifies that sequence length is 1576bp (such as sequence table SeqIDNo：Shown in 1), its sequence is committed to GenBank, with true
Determine the race relation of bacterial strain.Homology analysis result shows, the sequence and bacillus pumilus (Bacillus pumilus)
The homology highest of 16SrDNA sequences, conserved region similitude is 99%, by combining morphological features, growth conditions, physiology
Biochemical identification result determines that it belongs to bacillus, is bacillus pumilus (Bacillus pumilus).There are 100 sequences
Similitude with obtaining sequence is 99%, is bacillus, wherein it is bacillus pumilus (Bacillus to have 51 sequences
pumilus)。
By in the 16SrDNA sequences of bacterial strain and GenBank databases oneself know that bacterial strain 16SrDNA sequences carry out homology ratio
It is right, alignment is carried out with CLUSTALX programs, it is as shown in Figure 3 with MEGA5.05 software generation system chadograms.
Bacillus pumilus (Bacillus pumilus) the gy201401 degradation bacterias of the present invention are good in 28~38 DEG C of energy
Growth, optimum growth temperature are 36 DEG C；Bacterial strain can grow in pH4~9, optimal pH 6.0；Throughput is bigger, and thalli growth is got over
Good, it is one plant of aerobic bacteria to illustrate it.Its most suitable carbon nitrogen source is respectively sucrose and beef extract.
Bacillus pumilus (Bacillus pumilus) gy201401 bacterial strains of the present invention are in beef extract-peptone culture
On base, as shown in Fig. 2 degradation bacteria bacterium colony is circular, edge is approximate circle, and surface is smooth, and centre is yellow, opaque.
The present invention includes following beneficial effect：
Bacillus pumilus (Bacillus pumilus) gy201401 of the present invention is a kind of very strong micro- life of resistance
Thing, using the degraded that Atrazine is remained in significantly accelerated soil after this microorganism, Atrazine residual pair is alleviated or avoided
The harm of lower stubble sensitive crop, so as to improve the yield of crop, obtain higher economic benefit.
The present invention obtains the bacillus pumilus (Bacillus of one plant of Atrazine that can degrade by separation screening
Pumilus) gy201401, it can reach 36.14% to atrazine degradation rate.The effect of the strains for degrading Atrazine is not yet
Appear in the newspapers.In other reports, find pseudomonas, Bacillus cercus, aspergillus fumigatus, white-rot fungi and algae fibre etc. to Ah
Te Lajin has degradation.
The present invention to pedo relict Atrazine by applying the bacterium and plants sensitive crop beet simultaneously, further evaluation
Bacillus pumilus (Bacillus pumilus) gy201401 to the repair ability of Atrazine residual contamination in soil and
The damaging effect of crop is alleviated or avoided.Research shows, when Atrazine residual quantity is respectively 30 μ g/kg, 60 μ g/kg, applies
With the degradation bacteria, beet total solid yield increases by 24.42% and 60.98% respectively relative to the processing of no addition degradation bacteria,
1.5% and 4.71% are reduced respectively relative to blank；When Atrazine residual quantity is 90 μ g/kg, the sweet tea of the degradation bacteria is added
Dish total solid yield reduces by 15.32% relative to the processing increase by 413.46% of no addition degradation bacteria relative to blank.Thus
It can be seen that under this experimental condition, bacillus pumilus (Bacillus pumilus) gy201401 is applied in the drawing of low concentration Aunar
In the soil of Tianjin residual, poisoning effect can be completely eliminated, and high concentration Atrazine (90 μ g/kg) can be alleviated to the medicine of beet
Evil effect.From the point of view of the pedo relict Atrazine amount once determined, its residual quantity is both less than 50 μ g/kg, therefore, short and small gemma
Bacillus (Bacillus pumilus) gy201401 has Atrazine in efficient degradation soil, mitigates and even avoids Atrazine
Remain the toxic action to crop.
Brief description of the drawings
Fig. 1 is degradation bacteria gy201401 acquisition flow chart；
Fig. 2 is colonial morphologies of the degradation bacteria gy201401 on beef-protein medium；
Fig. 3 is degradation bacteria gy201401 systematic growth tree graph；
Fig. 4 is degradation bacteria gy201401BLAST comparison result figures；
Fig. 5 is upgrowth situation front view of the 14 days beet seedlings of emergence in various concentrations Atrazine residual；
Fig. 6 is upgrowth situation top view of the 14 days beet seedlings of emergence in various concentrations Atrazine residual；
Fig. 7 is upgrowth situation front view of the 18 days beet seedlings of emergence in various concentrations Atrazine residual；
Fig. 8 is upgrowth situation top view of the 18 days beet seedlings of emergence in various concentrations Atrazine residual；
Fig. 9 is upgrowth situation front view of the 26 days beet seedlings of emergence in various concentrations Atrazine residual；
Figure 10 is upgrowth situation top view of the 26 days beet seedlings of emergence in various concentrations Atrazine residual.
Embodiment
Embodiment one：The bacillus pumilus of one high-efficiency degradation Atrazine of present embodiment, it is short
Bacillus pumilus (Bacillus pumilus) gy201401, it is commonly micro- to be deposited in China Committee for Culture Collection of Microorganisms
Bio-Centers, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, and preservation date is on December 29th, 2014, preservation
Number it is CGMCCNo.10252.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several embodiments
Contract sample can also realize the purpose of invention.
Bacillus pumilus (Bacillus pumilus) gy201401 of present embodiment is to carry out in the following manner
(screening process figure is as shown in Figure 1) of screening：
First, tame：Added into soil (Heilongjiang University Hulan school district experimental plot is not by the black earth of pollution of herbicide)
10000 times of Atrazines, cultivated one month under conditions of temperature is 30 DEG C, humidity is 70%；
2nd, it is enriched with：The 5g soil samples after step 1 domestication are taken, are added to the 100mL containing the Atrazine that concentration is 100mg/L
In beef extract-peptone fluid nutrient medium, 250mL triangular flasks are subsequently placed in, the 160r/min vibrations training in 36 DEG C of shaken cultivation casees
Support 72h；
3rd, transfer：Nutrient solution after step 2 is enriched with by 10% switching amount be added to containing concentration is 100mg/L Ah
In Te Lajin basic inorganic salt fluid nutrient mediums (Atrazine makees only nitrogen source), it is above-mentioned that 100mL is added in 250mL triangular flasks
Culture medium, the then shaken cultivation under conditions of temperature is 36 DEG C, rotating speed is 160r/min (condition of culture is similarly hereinafter)；Taken per 72h
10% nutrient solution is inoculated into fresh minimal medium, while the Atrazine in the minimal medium cultivated every time contains
Amount all increases 100mg/L, and continuous domestication culture is until Atrazine final concentration increases to 600mg/L；
4th, solid medium selection culture：The bacterium solution that step 3 is finally cultivated does gradient dilution, to the bacterium of different gradients
Liquid respectively takes 100 μ L to be coated on basic inorganic salt solid medium flat board and (be used as only nitrogen source using Atrazine), in 36 DEG C of perseverances
Cultivated in warm incubator；
5th, aimed strain is filtered out：According to bacterium colony mode of appearance, the different bacterium colony of mode of appearance is picked out, by these not
Purified respectively three times with bacterium colony, obtain purifying bacterial strain.
6th, obtained purifying bacterial strain is subjected to degradation rate measure：Obtained purifying bacterial strain is prepared into bacteria suspension, is inoculated into
Containing concentration for 200mg/L Atrazines 50mL basic inorganic salt fluid nutrient mediums in, 160r/ in 36 DEG C of shaken cultivation casees
After min shaken cultivations 72h, with the residual quantity of high performance liquid chromatography detection Atrazine, degradation rate is measured after 36.14%, to receive
Collect bacterial strain；Wherein, liquid phase chromatogram condition：Chromatographic column (25cm × 4.6mm (id)) stainless steel column, inside fills out C18 (5 μm), temperature chamber
Temperature, Detection wavelength 222nm, mobile phase are methanol：Water=55：45, flow velocity, 0.7mL/min, appearance time about 17.3min.
The bacterial strain of collection is subjected to 16SrDNA identifications, sequence length is 1576bp (such as sequence table Seq ID No：1 institute
Show), its sequence is committed to GenBank, to determine the race relation of bacterial strain.Homology analysis result shows, the sequence with it is short and small
The homology highest of the 16SrDNA sequences of bacillus (Bacillus pumilus), conserved region similitude are 99%, pass through knot
Conjunction morphological features, growth conditions, Physiology and biochemistry qualification result determines that it belongs to bacillus, is bacillus pumilus
(Bacillus pumilus).The similitude for having 100 sequences and acquisition sequence is 99%, is bacillus, wherein having
51 sequences are bacillus pumilus (Bacillus pumilus).
By analysis above it was determined that the bacterial strain of screening is bacillus pumilus (Bacillus pumilus), I
Be named as bacillus pumilus (Bacillus pumilus) gy201401, and preservation is carried out to it, specific preservation information
For：Bacillus pumilus (Bacillus pumilus) gy201401, is deposited in China Committee for Culture Collection of Microorganisms
Common micro-organisms center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, and preservation date is December 29 in 2014
Day, preserving number is CGMCC No.10252.
Described basic inorganic salt culture medium component is：K2HPO40.5g, KH2PO40.5g, MgSO40.2g,
NaCl0.2g, glucose 5.0g, Atrazine are only nitrogen source, distilled water 1000mL, pH7.0 ± 0.2.
Bacillus pumilus (Bacillus pumilus) the gy201401 bacterial strains obtained to above-mentioned screening carry out function and tested
Card：
Bacillus pumilus (Bacillus pumilus) gy201401 degradation bacterias eliminate to the poisoning of Atrazine beet：
1st, test method
The atrazine concentration that this experiment is administered to soil is respectively 0,30,60,90,120 μ g/kg, according to pesticide concentration
The decoction prepared is uniformly sprayed on soil successively from low to high, soil is puddled the soil uniformly, prepared by 2% with decoction
Inoculum concentration is inoculated with bacillus pumilus (Bacillus pumilus) gy201401 bacterial strains respectively, while sets and add Atrazine to be not added with
The processing of bacterial strain and the clear water processing for being not added with Atrazine, each handle 3 repetitions, appropriate moisture content were supplemented every 3 days, keep one
Determine water content.Static culture is carried out at 25 DEG C one week.
The beet variety that this experiment is selected is gloomy for bus, selects the seed of full grains, and seed soaking is urged in insulating box after sterilization
Bud, after 3 day, the 12 beet seed kinds sprouted is selected into cultured soil per basin, are cultivated in illumination cultivation room,
Condition of culture：420 μm of 25 DEG C of day temperature, 19 DEG C of nocturnal temperature, humidity 60%, light radiation intensity olm-2s-1, illumination 14h/
My god.
The emergence of beet and situation of growing are observed, determines emergence rate and the death rate.
Emergence rate=(beet number of emerging/sowing number) × 100%；
The death rate=(beet death toll/number of emerging) × 100%；
Amount of dry matter, chlorophyll content and Net Photosynthetic Rate is measured by sampling within 26 days after emergence.
Measuring chlorophyll content instructs with reference to plant physiology experiment.
Photosynthetic rate (Pn) uses the portable photosynthetical system analysis-e/or determinings of LCA-4.
2nd, conclusion (of pressure testing)
2.1st, Atrazine and bacillus pumilus (Bacillus pumilus) gy201401 degradation bacterias go out to beet
The influence of seedling and survival condition.
Result of the test finds out, blank control emergence rate is 97.22%, the emergence rate of Atrazine processing by concentration from it is low to
High order is followed successively by 97.22%, 94.44%, 94.44%, 94.44%, the emergence rate being vaccinated with after degradation bacteria gy201401
Respectively 94.44%, 94.44%, 97.22%, 97.22%.By the different processing of variance analysis, difference is not compared with the control
Substantially.This shows that emergence of the various concentrations Atrazine of experimental design to beet does not have a significant effect；The addition of bacterium solution
Beet emergence is not had an impact.Therefore under this experimental condition, degradation bacteria does not have shadow with emergence of the Atrazine to beet yet
Ring.
(Y2 in figure refers to bacillus pumilus (Bacillus pumilus) gy201401) is understood by Fig. 5~10, though
The influence of Te Lajin and degradation bacteria to emergence rate is little in right Ah's soil, but the growth to seedling starts to produce over time
It is raw to influence.When emerging 14 days, Atrazine residual quantity is poisoning and unobvious in 60 μ g/kg processing in soil；But 90
In μ g/kg processing, existing most of sweet tea in not handled using bacillus pumilus (Bacillus pumilus) gy201401
Dish death of seedling, and beet seedling does not occur for the processing for applying bacillus pumilus (Bacillus pumilus) gy201401
The phenomena of mortality.
When emerging 18 days, Atrazine residual quantity is in 60 μ g/kg processing in soil, has been able to find out poisoning pair
Beet seedling growth generates influence；In 90 μ g/kg processing, not using bacillus pumilus (Bacillus pumilus)
The beet seedling growth of gy201401 processing is suppressed by serious, and applies bacillus pumilus (Bacillus pumilus)
The beet seedling of gy201401 processing is grown only by slight inhibitory action.
When emerging 26 days, Atrazine residual quantity is in 60 μ g/kg processing in soil, can will become apparent from Aunar drawing
Poisoning phenomenon of the Tianjin to beet, bacillus pumilus (Bacillus pumilus) gy201401 processing is administered simultaneously and fails to understand
It is aobvious；In 90 μ g/kg processing, the beet not handled using bacillus pumilus (Bacillus pumilus) gy201401 is young
Seedling does not almost grow, and applies the beet seedling of bacillus pumilus (Bacillus pumilus) gy201401 processing with right
Photograph ratio, grows fine.
2.2nd, the children of Atrazine and bacillus pumilus (Bacillus pumilus) gy201401 degradation bacterias to beet
The influence of seedling dry matter.
2.2.1 influence of the Atrazine to the seedling dry matter of beet.
Atrazine poisoning results from pot experiment test shows, the emergence of the administration of Atrazine to beet does not have an impact, but
There is very big harm to beet seedling, to after being emerged when harvesting 26 days, when atrazine concentration is 120 μ g/kg, beet children
Seedling is all dead；When atrazine concentration is 90 μ g/kg, the death rate of beet seedling is 86.11%；The Atrazine of low concentration
Even if not resulting in beet seedling death, but also seriously reduce sugar beet seedlings bio.Beet is in Atrazine residual concentration
For 30 μ g/kg, 60 μ g/kg and 90 μ g/kg when, total solid yield reduces by 18.42%, 40.81% and relative to blank respectively
83.51%.
2.2.2 the seedling dry of each processing beet after bacillus pumilus (Bacillus pumilus) gy201401 is applied
Accumulation.
When Atrazine residual quantity is 30 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet total solid yield is relative to no addition bacillus pumilus (Bacillus pumilus) gy201401
The beet total solid yield 24.42% of processing, increase by 1.50% relative to blank.Bacillus pumilus (Bacillus
Pumilus) gy201401 administration completely eliminates poisoning effect of the Atrazine to beet.
When Atrazine residual quantity is 60 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet total solid yield is relative to no addition bacillus pumilus (Bacillus pumilus) gy201401
The beet total solid yield 60.98% of processing, distinguish 4.71% relative to blank.Bacillus pumilus (Bacillus
Pumilus) gy201401 administration almost eliminates poisoning effect of the Atrazine to beet.
When Atrazine residual quantity is 90 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet total solid yield is relative to no addition bacillus pumilus (Bacillus pumilus) gy201401
The beet total solid yield 413.46% of processing, 15.32% is reduced relative to blank.Bacillus pumilus (Bacillus
Pumilus) gy201401 administration, poisoning effect of the Atrazine to beet can be alleviated.
2.3rd, Atrazine and bacillus pumilus (Bacillus pumilus) gy201401 are photosynthetic to beet
Influence
2.3.1, Atrazine and bacillus pumilus (Bacillus pumilus) gy201401 are green to beet leaf leaf
The influence of cellulose content
Beet when Atrazine residual concentration is 30 μ g/kg, 60 μ g/kg and 90 μ g/kg, Chlorophyll content relative to
Blank reduces 5.34%, 8.75% and 11.86% respectively.
When Atrazine residual quantity is 30 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet Chlorophyll content increases by 6.10% relative to the beet total solid yield of no addition degradation bacteria processing,
Increase by 0.44% relative to blank；When Atrazine residual quantity is 60 μ g/kg, bacillus pumilus (Bacillus is added
Pumilus) gy201401 beet Chlorophyll content is relative to no addition bacillus pumilus (Bacillus
Pumilus) the beet total solid yield increase by 9.54% of gy201401 processing, 0.04% is reduced relative to blank；Drawn in Aunar
When Tianjin residual quantity is 90 μ g/kg, bacillus pumilus (Bacillus pumilus) gy201401 beet Chlorophyll is added
Content contains relative to no addition bacillus pumilus (Bacillus pumilus) gy201401 beet Chlorophylls handled
Amount increase by 12.48%, 0.86% is reduced relative to blank.
Thus illustrate, formation of the Atrazine residual to beet chlorophyll has inhibitory action, bacillus pumilus
(Bacillus pumilus) gy201401 eliminates Atrazine when Atrazine residual quantity is 30 μ g/kg and 60 μ g/kg
To the inhibitory action of beet chlorophyll formation, inhibitory action is alleviated in 90 μ g/kg.
2.3.2, Atrazine and bacillus pumilus (Bacillus pumilus) gy201401 are to the net light of beet leaf
Close the influence of speed
Beet is when Atrazine residual concentration is 30 μ g/kg, 60 μ g/kg and 90 μ g/kg, the Net Photosynthetic Rate phase of beet
Reduce 2.18%, 10.13% and 16.76% respectively for blank.
When Atrazine residual quantity is 30 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet Net Photosynthetic Rate is relative to no addition bacillus pumilus (Bacillus pumilus) gy201401
The beet Net Photosynthetic Rate increase by 2.55% of processing, increases by 0.31% relative to blank；It is 60 μ g/kg in Atrazine residual quantity
When, the beet Net Photosynthetic Rate for adding bacillus pumilus (Bacillus pumilus) gy201401 is short relative to no addition
The beet Net Photosynthetic Rate increase by 8.50% of bacillus pumilus (Bacillus pumilus) gy201401 processing, relative to blank
Reduce by 2.49%；When Atrazine residual quantity is 90 μ g/kg, bacillus pumilus (Bacillus pumilus) is added
Gy201401 beet Net Photosynthetic Rate is relative to no addition bacillus pumilus (Bacillus pumilus) gy201401
The beet Net Photosynthetic Rate increase by 7.77% of processing, 10.29% is reduced relative to blank.
Thus illustrate, Atrazine remains has inhibitory action, bacillus pumilus to the Net Photosynthetic Rate of beet
It is net to beet to eliminate Atrazine when Atrazine residual quantity is 30 μ g/kg by (Bacillus pumilus) gy201401
The inhibitory action of photosynthetic rate, inhibitory action is alleviated in 60 μ g/kg and 90 μ g/kg.
The pollution that bacillus pumilus (Bacillus pumilus) gy201401 is applied to Atrazine residual has aobvious
The repair of work, bacillus pumilus (Bacillus pumilus) gy201401 administration can ensure sensitive crop beet
Normal total solid yield, chlorophyll content and net light and speed.Under this experimental condition, bacillus pumilus (Bacillus
Pumilus) gy201401 is applied in the soil of low concentration Atrazine residual, poisoning effect can be completely eliminated, and can delay
Solve poisoning effect of the high concentration Atrazine (90 μ g/kg) to beet.
Claims (1)
1. the bacillus pumilus of a high-efficiency degradation Atrazine, it is characterised in that it is bacillus pumilus (Bacillus
Pumilus) gy201401, is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation address is
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date are on December 29th, 2014, and preserving number is CGMCC No.10252.
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| CN105802874B (en) * | 2015-11-03 | 2019-11-19 | 山东鲁虹智慧农业研究院 | A kind of Mixed Microbes and fermentation culture method of efficient degradation Atrazine |
| CN108118018B (en) * | 2018-02-09 | 2020-10-02 | 广东海洋大学 | Bacillus aryabhattai W-5 and application thereof |
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