CN108408922A - A method of repairing organochlorine pesticide pollution water body using composite bacteria agent - Google Patents
A method of repairing organochlorine pesticide pollution water body using composite bacteria agent Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention belongs to microorganisms technical fields, disclose a kind of method for repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, between adjusting pH is 6.5 7.5；Subsequently into biological reaction pool, control water temperature is between 20 35 DEG C, by composite bacteria agent according to 1 5g/m3Additive amount put into biological reaction pool, processing time be 3 6h, then filtered by plate filter, be finally discharged.The method of the present invention efficiently can quickly handle organochlorine contamination water body.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of to repair organochlorine pesticide pollution water using composite bacteria agent
The method of body.
Background technology
Organo-chlorine pesticide is for preventing the organic compound for containing organic chlorine element in the constituent of phytopathy, insect pest
Object.It is raw material and using cyclopentadiene as the two major classes of raw material to be broadly divided into using benzene.The former is as used earliest, most widely used desinsection
Agent DDT and six six six and acaricide tetradiphon, dicofol etc., fungicide pentachloronitrobenzene, Bravo, Dao Feng
It is peaceful etc.；The latter is such as the Niran of insecticide, heptachlor, drinox.In addition using turpentine oil as amphene insecticides, the poison of raw material
It kills sweet smell and also belongs to organo-chlorine pesticide by the bornyl chloride of raw material of terpenes.Common organo-chlorine pesticide has plurality of properties：1. making
It is disappeared slowly with rear；2. fat-soluble strong；3. chlorobenzene framework is stablized, it is not easy as body enzymatic degradation, disappears in vivo slow；④
The product of edaphon effect, the same there is residual toxicity as well as parent, such as DDT generates DDD, dechlorinatedization hydrogen through restoring
After generate DDE；5. some organo-chlorine pesticides can be evaporated if DDT can be suspended in the water surface with hydrone.Organochlorine in environment
Pesticide is acted on by biological concentration and food chain, harm biology.Nerve center is mainly stimulated to the acute toxicity of people, it is chronic
Poisoning manifestations are loss of appetite, and weight loss can also generate small brain disorder, blood forming organ obstacle etc. sometimes.Document report has
Organo-chlorine pesticide has carcinogenicity to experimental animal.Chlorobenzene structure relatively stablize, enzyme is difficult to degrade in organism, thus accumulate in it is dynamic,
Organo-chlorine pesticide molecule in plant disappears slow.Due to this characteristic, it passes through the effect of biological concentration and food chain, ring
Remains of pesticide in border can further obtain agriculture collection and diffusion.By food chain enter human body organo-chlorine pesticide can liver, kidney,
Accumulated in the tissues such as heart, especially because this kind of pesticide is fat-soluble big, so in vivo in fat store up it is more prominent.Accumulation
Remains of pesticide can be also discharged by breast milk, or be transferred to the tissues such as ovum egg, influence offspring.
Organo-chlorine pesticide can be entered with rainwater in river and lake, be polluted to environment.Currently, the processing used
Method includes mainly chemical treatment and biological renovation method.Wherein, chemical treatment method is quick, but cost is higher, and
It is easy to cause secondary pollution；Biological renovation method is more much lower than chemical treatment method cost, non-secondary pollution, but for continuing
Organo-chlorine pesticide serious pollution place, organo-chlorine pesticide are not easy to utilize for single microorganism, need to carry out compatibility to microorganism,
And by microorganism, respectively the influence factors such as nutritional mode and metabolic way are more, how hybrid bacterial strain will appear it is completely different
Effect, it is difficult to the composite bacteria agent that compatibility rationally mutually cooperates with is obtained, to cannot achieve the effect for quickly removing pollutant.China
Patent of invention " CN107364978A " is disclosed a kind of adsorbed using Nanoscale Iron and whiterot fungi and removes eluent organo-chlorine pesticide
Method, this method by enzyme inducer of degrading enhance survival of the whiterot fungi in the synergy eluent of concentrated surfactant and
Growth, is adjusted by nutritional ingredient, under Nanoscale Iron catalytic action, fast degradation Tween 80, polysorbas20 and triton X-100 etc.
Surfactant forms the absorption to organo-chlorine pesticide under the environment such as suitable temperature, organic for high concentration in 10 days
Chloro pesticide contaminated soil eluent removes efficiency and reaches 90% or more.The above method use chemical method assist biological method into
Row processing, effect is preferable, but high expensive, is not easy to promote the use of.It develops at present a kind of organic using composite bacteria agent reparation
The method of chloro pesticide polluted-water is our technical issues that need to address.
Invention content
In order to overcome the deficiencies of existing technologies, organochlorine agriculture is repaired using composite bacteria agent the object of the present invention is to provide a kind of
The method of chemicals contamination water body.
The present invention adopts the following technical scheme that realization：
A method of repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：
By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, adjust
PH is between 6.5-7.5；Subsequently into biological reaction pool, control water temperature is between 20-35 DEG C, by composite bacteria agent according to 1-
5g/m3Additive amount put into biological reaction pool, then processing time 3-6h is filtered, most heel row by plate filter
Go out.
Further,
The composite bacteria agent is prepared according to following technique：
Bacillus subtilis-human pallid bacillus mixed fermentation liquid, Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis zymotic fluid are pressed
According to 2-3:1-2:The volume ratio of 1-2 is uniformly mixed, and obtains composite bacteria liquid；Then pass through vacuum freeze drying and composite bacteria agent is made.
Further,
The preparation method of the bacillus subtilis-human pallid bacillus mixed fermentation liquid includes the following steps：
First bacillus subtilis seed liquor is linked into according to 6% inoculum concentration in fermentation medium, 30 DEG C cultivate 6 hours, then
Human pallid bacillus seed liquor is linked into fermentation medium according to 8% inoculum concentration, continues to cultivate 18 hours with 30 DEG C, obtain withered
Careless bacillus-human pallid bacillus mixed fermentation liquid；The group of the fermentation medium is divided into：Glucose 12g/L, yeast extract 5g/
L, ammonium sulfate 3g/L, dipotassium hydrogen phosphate 1g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran
0.2mg/L。
Further,
The preparation method of the Phanerochaete chrysosporium zymotic fluid includes the following steps：
Phanerochaete chrysosporium seed liquor is linked into according to 10% inoculum concentration in fermentation medium, 30 DEG C of cultures for 24 hours, obtain
Phanerochaete chrysosporium zymotic fluid；The formula of the fermentation medium is：Molasses 10g/L, dregs of beans 5g/L, urea 2g/L, chlorination
Calcium 0.1g/L, manganese sulfate 0.01g/L, Niran 0.1mg/L.
Further,
The preparation method of the Alcaligenes faecalis zymotic fluid includes the following steps：
Alcaligenes faecalis seed liquor is linked into according to the inoculum concentration of 8% volume ratio in fermentation medium, 30 DEG C of cultures for 24 hours, obtain excrement
Alcaligenes zymotic fluid；The formula of the fermentation medium is：Glucose 12g/L, ammonium sulfate 5g/L, dipotassium hydrogen phosphate 1g/L, phosphorus
Acid dihydride potassium 1g/L, sodium chloride 0.1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran 0.1mg/L.
Preferably,
The bacillus subtilis is ATCC39085, and the human pallid bacillus is ATCC49188, the Phanerochaete chrysosporium
For ATCC34540, the Alcaligenes faecalis is ATCC19209.
Compared with prior art, the advantageous effect that technical solution of the present invention obtains includes mainly the following aspects：
The method of the present invention is simple and fast, can effectively reduce the content of organochlorine pollutant；The composite bacteria agent choosing that the present invention uses
It selects and uses four kinds of bacterial strains, compatibility is reasonable, and synergisticing performance is good, by Co metabolism relationship between bacterial strain, can form efficient metabolism
Degradation system, is compared compared with single bacterial strain, can significantly improve the efficiency of degradation organo-chlorine pesticide；The present invention utilizes composite bacteria agent
The method for handling agricultural chemicals waste water is at low cost, environment friendly and pollution-free, has a extensive future；Bacterial strain of the present invention first passes around domestication culture,
Environment can be more rapidly adapted to, the efficiency of degradation organo-chlorine pesticide is improved.
Specific implementation mode
Those skilled in the art can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.The product and method of the present invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified or are suitably changed and combined in bright content, spirit and scope, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Experimental method in following embodiments is unless otherwise specified conventional method.Examination as used in the following examples
Material is tested, is to be commercially available from conventional biochemical reagent channel unless otherwise specified.
Embodiment 1
A method of repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：
By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, adjust
PH is between 6.5-7.5；Subsequently into biological reaction pool, control water temperature is between 26 DEG C, by composite bacteria agent according to 1g/m3
Additive amount put into biological reaction pool, then processing time 5h is filtered by plate filter, is finally discharged.
The composite bacteria agent is prepared according to following technique：
Bacillus subtilis-human pallid bacillus mixed fermentation liquid, Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis zymotic fluid are pressed
According to 2:1:1 volume ratio is uniformly mixed, and obtains composite bacteria liquid；Then pass through vacuum freeze drying and composite bacteria agent is made.
The preparation method of the bacillus subtilis-human pallid bacillus mixed fermentation liquid includes the following steps：
First by bacillus subtilis seed liquor according to 6% inoculum concentration（Inoculum density is 1 × 107CFU/ml）It is linked into fermentation training
Support base in, 30 DEG C cultivate 6 hours, then by human pallid bacillus seed liquor according to 8% inoculum concentration（Inoculum density be 1 ×
107CFU/ml）It is linked into fermentation medium, continues to cultivate 18 hours with 30 DEG C, it is mixed to obtain bacillus subtilis-human pallid bacillus
Close zymotic fluid；The group of the fermentation medium is divided into：Glucose 12g/L, yeast extract 5g/L, ammonium sulfate 3g/L, dipotassium hydrogen phosphate
1g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran 0.2mg/L.
The preparation method of the Phanerochaete chrysosporium zymotic fluid includes the following steps：
By Phanerochaete chrysosporium seed liquor according to 10% inoculum concentration（Inoculum density is 1 × 107CFU/ml）It is linked into fermentation training
It supports in base, 30 DEG C of cultures for 24 hours, obtain Phanerochaete chrysosporium zymotic fluid；The formula of the fermentation medium is：Molasses 10g/L,
Dregs of beans 5g/L, urea 2g/L, calcium chloride 0.1g/L, manganese sulfate 0.01g/L, Niran 0.1mg/L.
The preparation method of the Alcaligenes faecalis zymotic fluid includes the following steps：
By Alcaligenes faecalis seed liquor according to the inoculum concentration of 8% volume ratio（Inoculum density is 1 × 107CFU/ml）It is linked into fermentation training
It supports in base, 30 DEG C of cultures for 24 hours, obtain Alcaligenes faecalis zymotic fluid；The formula of the fermentation medium is：Glucose 12g/L, sulfuric acid
Ammonium 5g/L, dipotassium hydrogen phosphate 1g/L, potassium dihydrogen phosphate 1g/L, sodium chloride 0.1g/L, magnesium sulfate 0.01g/L, ferrous sulfate
0.01g/L, Niran 0.1mg/L.
The bacillus subtilis is ATCC39085, and the human pallid bacillus is ATCC49188, and the Huang archespore hair is flat
Lead fungi is ATCC34540, and the Alcaligenes faecalis is ATCC19209.
Embodiment 2
A method of repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：
By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, adjust
PH is between 6.5-7.5；Subsequently into biological reaction pool, control water temperature is between 28 DEG C, by composite bacteria agent according to 2g/m3
Additive amount put into biological reaction pool, then processing time 3h is filtered by plate filter, is finally discharged.
The composite bacteria agent is prepared according to following technique：
Bacillus subtilis-human pallid bacillus mixed fermentation liquid, Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis zymotic fluid are pressed
According to 3:2:2 volume ratio is uniformly mixed, and obtains composite bacteria liquid；Then pass through vacuum freeze drying and composite bacteria agent is made.
The preparation method of the bacillus subtilis-human pallid bacillus mixed fermentation liquid includes the following steps：
First bacillus subtilis seed liquor is linked into according to 6% inoculum concentration in fermentation medium, 30 DEG C cultivate 6 hours, then
Human pallid bacillus seed liquor is linked into fermentation medium according to 8% inoculum concentration, continues to cultivate 18 hours with 30 DEG C, obtain withered
Careless bacillus-human pallid bacillus mixed fermentation liquid；The group of the fermentation medium is divided into：Glucose 12g/L, yeast extract 5g/
L, ammonium sulfate 3g/L, dipotassium hydrogen phosphate 1g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran
0.2mg/L。
The preparation method of the Phanerochaete chrysosporium zymotic fluid includes the following steps：
Phanerochaete chrysosporium seed liquor is linked into according to 10% inoculum concentration in fermentation medium, 30 DEG C of cultures for 24 hours, obtain
Phanerochaete chrysosporium zymotic fluid；The formula of the fermentation medium is：Molasses 10g/L, dregs of beans 5g/L, urea 2g/L, chlorination
Calcium 0.1g/L, manganese sulfate 0.01g/L, Niran 0.1mg/L.
The preparation method of the Alcaligenes faecalis zymotic fluid includes the following steps：
Alcaligenes faecalis seed liquor is linked into according to the inoculum concentration of 8% volume ratio in fermentation medium, 30 DEG C of cultures for 24 hours, obtain excrement
Alcaligenes zymotic fluid；The formula of the fermentation medium is：Glucose 12g/L, ammonium sulfate 5g/L, dipotassium hydrogen phosphate 1g/L, phosphorus
Acid dihydride potassium 1g/L, sodium chloride 0.1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran 0.1mg/L.
The bacillus subtilis is ATCC39085, and the human pallid bacillus is ATCC49188, and the Huang archespore hair is flat
Lead fungi is ATCC34540, and the Alcaligenes faecalis is ATCC19209.
Embodiment 3
A method of repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：
By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, adjust
PH is between 6.5-7.5；Subsequently into biological reaction pool, control water temperature is between 30 DEG C, by composite bacteria agent according to 4g/m3
Additive amount put into biological reaction pool, then processing time 4h is filtered by plate filter, is finally discharged.
The composite bacteria agent is prepared according to following technique：
Bacillus subtilis-human pallid bacillus mixed fermentation liquid, Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis zymotic fluid are pressed
According to 3:2:1 volume ratio is uniformly mixed, and obtains composite bacteria liquid；Then pass through vacuum freeze drying and composite bacteria agent is made.
The preparation method of the bacillus subtilis-human pallid bacillus mixed fermentation liquid includes the following steps：
First bacillus subtilis seed liquor is linked into according to 6% inoculum concentration in fermentation medium, 30 DEG C cultivate 6 hours, then
Human pallid bacillus seed liquor is linked into fermentation medium according to 8% inoculum concentration, continues to cultivate 18 hours with 30 DEG C, obtain withered
Careless bacillus-human pallid bacillus mixed fermentation liquid；The group of the fermentation medium is divided into：Glucose 12g/L, yeast extract 5g/
L, ammonium sulfate 3g/L, dipotassium hydrogen phosphate 1g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran
0.2mg/L。
The preparation method of the Phanerochaete chrysosporium zymotic fluid includes the following steps：
Phanerochaete chrysosporium seed liquor is linked into according to 10% inoculum concentration in fermentation medium, 30 DEG C of cultures for 24 hours, obtain
Phanerochaete chrysosporium zymotic fluid；The formula of the fermentation medium is：Molasses 10g/L, dregs of beans 5g/L, urea 2g/L, chlorination
Calcium 0.1g/L, manganese sulfate 0.01g/L, Niran 0.1mg/L.
The preparation method of the Alcaligenes faecalis zymotic fluid includes the following steps：
Alcaligenes faecalis seed liquor is linked into according to the inoculum concentration of 8% volume ratio in fermentation medium, 30 DEG C of cultures for 24 hours, obtain excrement
Alcaligenes zymotic fluid；The formula of the fermentation medium is：Glucose 12g/L, ammonium sulfate 5g/L, dipotassium hydrogen phosphate 1g/L, phosphorus
Acid dihydride potassium 1g/L, sodium chloride 0.1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran 0.1mg/L.
The bacillus subtilis is ATCC39085, and the human pallid bacillus is ATCC49188, and the Huang archespore hair is flat
Lead fungi is ATCC34540, and the Alcaligenes faecalis is ATCC19209.
Embodiment 4
Water remediation is tested：
Operating process is with reference to embodiment 1, and the content of Niran is 0.43mg/L in water body.
Composite bacteria agent group：Group 1：Embodiment 1；Group 2：Only with bacillus subtilis-human pallid bacillus mixed fermentation liquid,
Remaining method with embodiment 1；Group 3：Only with Phanerochaete chrysosporium zymotic fluid, remaining method with embodiment 1；Group 4：Only
Using Alcaligenes faecalis zymotic fluid, remaining method with embodiment 1；Group 5：Using Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis
Two kinds of bacterium solutions of zymotic fluid, remaining method with embodiment 1.Specific handling result is shown in Table 1：
Table 1
| Group | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 |
| Niran concentration mg/L | 0 | 0.11 | 0.19 | 0.21 | 0.13 |
Conclusion：As shown in table 1, composite bacteria agent handles 5h, and the presence of Niran is can't detect in group 1, is realized degradable；It uses
The group 3 and 4 effects of group of single bacterium agent are poor, reduce 56% and 51% respectively, group 2 and group 5 are all made of two kinds of microbial inoculums, and effect is better than
The group 3 and group 4 of single bacterium agent, but it is poor compared with using 1 effect of group of four kinds of bacterial strains.
Embodiment 4
The present invention also detects the degradation effect to 5a,6,9,9a-hexahydro-6,9-methano-2,4, and operating process is the same as embodiment 2.The a concentration of of 5a,6,9,9a-hexahydro-6,9-methano-2,4 in water body is set
0.1mg/L；Group setting is with reference to embodiment 3.Concrete outcome is shown in Table 2：
Table 2
| Group | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 |
| 5a,6,9,9a-hexahydro-6,9-methano-2,4 concentration mg/L | 0.006 | 0.017 | 0.036 | 0.045 | 0.022 |
Conclusion：As shown in table 2, compared with group 2-5, organize 1 degradation effect it is best, can reach 94%, group 3 and group 4 use single bacterium
The degradation effect of agent is worst, and only 64% and 55%, group 2 and group 5 use two kinds of bacterial strain compatibilities, effect to be better than single microbial inoculum, still
Degradation effect is obviously not so good as the group 1 using four kinds of bacterial strains.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specific example " or
The description of " some examples " etc. means that specific features, material or feature described in conjunction with this embodiment or example are contained in this hair
In bright at least one embodiment or example.In the present specification, schematic expression of the above terms are necessarily directed to
Identical embodiment or example.Although the embodiments of the present invention has been shown and described above, it is to be understood that above-mentioned reality
It applies example to be exemplary, be not considered as limiting the invention, those skilled in the art are within the scope of the invention
It can make changes, modifications, alterations, and variations to the above described embodiments.
Claims (6)
1. a kind of method for repairing organochlorine pesticide pollution water body using composite bacteria agent comprising following steps：
By organochlorine pesticide pollution water body first by fence, fence clearance control is within 1mm；Again by regulating reservoir, adjust
PH is between 6.5-7.5；Subsequently into biological reaction pool, control water temperature is between 20-35 DEG C, by composite bacteria agent according to 1-
5g/m3Additive amount put into biological reaction pool, then processing time 3-6h is filtered, most heel row by plate filter
Go out.
2. according to the method described in claim 1, it is characterized in that, the composite bacteria agent is prepared according to following technique：
Bacillus subtilis-human pallid bacillus mixed fermentation liquid, Phanerochaete chrysosporium zymotic fluid, Alcaligenes faecalis zymotic fluid are pressed
According to 2-3:1-2:The volume ratio of 1-2 is uniformly mixed, and obtains composite bacteria liquid；Then pass through vacuum freeze drying and composite bacteria agent is made.
3. according to the method described in claim 2, it is characterized in that, the bacillus subtilis-human pallid bacillus mixed fermentation
The preparation method of liquid includes the following steps：
First bacillus subtilis seed liquor is linked into according to 6% inoculum concentration in fermentation medium, 30 DEG C cultivate 6 hours, then
Human pallid bacillus seed liquor is linked into fermentation medium according to 8% inoculum concentration, continues to cultivate 18 hours with 30 DEG C, obtain withered
Careless bacillus-human pallid bacillus mixed fermentation liquid；The group of the fermentation medium is divided into：Glucose 12g/L, yeast extract 5g/
L, ammonium sulfate 3g/L, dipotassium hydrogen phosphate 1g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran
0.2mg/L。
4. according to the method described in claim 2, it is characterized in that, the preparation method packet of the Phanerochaete chrysosporium zymotic fluid
Include following steps：
Phanerochaete chrysosporium seed liquor is linked into according to 10% inoculum concentration in fermentation medium, 30 DEG C of cultures for 24 hours, obtain
Phanerochaete chrysosporium zymotic fluid；The formula of the fermentation medium is：Molasses 10g/L, dregs of beans 5g/L, urea 2g/L, chlorination
Calcium 0.1g/L, manganese sulfate 0.01g/L, Niran 0.1mg/L.
5. according to the method described in claim 2, it is characterized in that, the preparation method of the Alcaligenes faecalis zymotic fluid includes as follows
Step：
Alcaligenes faecalis seed liquor is linked into according to the inoculum concentration of 8% volume ratio in fermentation medium, 30 DEG C of cultures for 24 hours, obtain excrement
Alcaligenes zymotic fluid；The formula of the fermentation medium is：Glucose 12g/L, ammonium sulfate 5g/L, dipotassium hydrogen phosphate 1g/L, phosphorus
Acid dihydride potassium 1g/L, sodium chloride 0.1g/L, magnesium sulfate 0.01g/L, ferrous sulfate 0.01g/L, Niran 0.1mg/L.
6. according to the method described in claim 2-6, which is characterized in that the bacillus subtilis is ATCC39085, the people
Anthropi is ATCC49188, and the Phanerochaete chrysosporium is ATCC34540, and the Alcaligenes faecalis is ATCC19209.
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| CN201810326918.3A Pending CN108408922A (en) | 2018-04-12 | 2018-04-12 | A method of repairing organochlorine pesticide pollution water body using composite bacteria agent |
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