CN108397951A - A kind of fresh cocoon store method of sample - Google Patents

A kind of fresh cocoon store method of sample Download PDF

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Publication number
CN108397951A
CN108397951A CN201810015294.3A CN201810015294A CN108397951A CN 108397951 A CN108397951 A CN 108397951A CN 201810015294 A CN201810015294 A CN 201810015294A CN 108397951 A CN108397951 A CN 108397951A
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China
Prior art keywords
cocoon
fresh
mesh bag
fresh cocoon
store method
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CN201810015294.3A
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Chinese (zh)
Inventor
江文斌
王玲玲
邢秋明
叶飞
杨娟亚
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HUZHOU CITY FIBER TESTING
Zhejiang Sci Tech University ZSTU
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HUZHOU CITY FIBER TESTING
Zhejiang Sci Tech University ZSTU
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Priority to CN201810015294.3A priority Critical patent/CN108397951A/en
Publication of CN108397951A publication Critical patent/CN108397951A/en
Pending legal-status Critical Current

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    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D11/00Self-contained movable devices, e.g. domestic refrigerators

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to storage technique fields, and to solve the problems of fresh cocoon long-term preservation of use for laboratory, the present invention proposes a kind of fresh cocoon store method of sample:The fresh cocoon of rigidifying pupa is packed into cocoon basket in constant-temperature constant-humidity environment;Fresh cocoon is dispensed into hollow out mesh bag, each mesh bag fills fresh cocoon 100 200;The mesh bag for installing fresh cocoon layering is suspended in refrigerator-freezer side by side before silkworm chrysalis moth;Before silk cocoon use, mesh bag is taken out from refrigerator-freezer, is placed in room temperature environment after thawing 23 hours and uses.Method is simple, cheap, exploitativeness is strong, and can keep the freshness of silkworm chrysalis the long period, preserves cocoon quality, and effectively improves unwinding, drop low filature fold, suitable for the fresh cocoon of long-term preservation small lot.

Description

A kind of fresh cocoon store method of sample
Technical field
The present invention relates to storage technique field, more particularly to the fresh cocoon store method of a kind of sample.
Background technology
For a long time, it is all made of high-temperature heating drying in order to preserve after silk cocoon prevents the moth that goes mouldy and go out, fresh cocoon purchase from coming in, The silkworm chrysalis in cocoon layer is killed, moisture removal is removed, to use throughout the year.But not only complex procedures, cost and energy consumption are big for cocoon drying, And xeothermic cocoon drying pupa killing can cause sericin to be denaturalized, and silk gum solubility reduces to influence the unwinding of silk cocoon, increases reel silk from cocoons Folding.In addition, cocoon drying process can make the albumen of fresh pupa and fatty heat damage, specific peculiar smell is generated, to influence answering for silkworm chrysalis With the cocoon quality of value and silk cocoon, and then the quality of raw silk is influenced, therefore fresh cocoon preserves technique and is just particularly important.
In addition, for the needs that laboratory researcher studies for a long period of time, how small lot preserves silk cocoon, makes it without baking Cocoon is just avoided that silkworm chrysalis moth, and the freshness of the holding silk cocoon of long period, is the key technical problem that must be captured. According to the characteristic of the property of protein and sericin, it just can guarantee that silk gum invariance, silkworm chrysalis are fresh in a cold or frozen state.Fresh cocoon Preserve process has very important influence to the yield and quality etc. of silk cocoon cocoon quality, unwinding, reel silk from cocoons folding, raw silk.When fresh cocoon preserves When temperature is higher than to a certain degree, fresh cocoon cannot preserve well, and when refrigerated storage temperature is less than to a certain degree, the developmental phase of fresh pupa is worked as Slowly, can keep fresh in a longer period of time, but also can not the fresh pupa of digital preservation freshness, the holding time compared with When long, it may appear that the fresh pupa in part is freezed to death and pupal cell darkening of color situation.
Invention content
To solve the problems of fresh cocoon long-term preservation of use for laboratory, the present invention proposes a kind of sample and is preserved with fresh cocoon Method, method is simple, cheap, exploitativeness is strong, and can keep the freshness of silkworm chrysalis the long period, preserves cocoon quality, and Effectively improve unwinding, drop low filature fold, suitable for the fresh cocoon of long-term preservation small lot.
The present invention is achieved by the following technical solutions:A kind of sample is following steps with fresh cocoon store method:
(1)The fresh cocoon of rigidifying pupa is packed into cocoon basket in constant-temperature constant-humidity environment,
Preferably, preservation condition is 18-25 DEG C of temperature, humidity 60-75%.
Preferably, the cocoon basket is the product that each face is breathed freely.
Preferably, fresh cocoon amount is the 2/5-4/5 of cocoon basket total capacity.It can prevent silk cocoon in cocoon basket from stacking too full, pupal cell The heat and moisture that respiration and urination generate make silk cocoon is interior in a limited space to turn to be yellow because of hyther, influence cocoon quality,
Preferably, the silk cocoon being placed in cocoon basket was gradually decreased from four weeks to cocoon basket center, arrange in concave.
Preferably, the fresh cocoon of rigidifying pupa that purchase is come rejects the spoiled cocoon for being unsatisfactory for requirement of experiment in 7, to prevent influencing The quality of other silk cocoons, the silk cocoon after generally pupating changed moth at 13-14 days.
(2)Fresh cocoon is dispensed into hollow out mesh bag, each mesh bag fills fresh cocoon 100-200;
Preferably, fresh cocoon amount is the 1/3-2/3 of mesh bag total capacity.
(3)The mesh bag for installing fresh cocoon layering is suspended in refrigerator-freezer side by side before silkworm chrysalis moth;
Preferably, the mesh bag for installing fresh cocoon layering is suspended in refrigerator-freezer side by side in 10 days at least after silkworm pupates.
Preferably, the temperature setting of refrigerator-freezer is -15 DEG C ~ -25 DEG C.The reelability percentage of the fresh cocoon preserved at this temperature, solution Shu Sichang, myriametre hang rough index and are superior to baked cocoom.In addition, the fresh cocoon preserved in this temperature range can be with the holding of long period The freshness of silkworm chrysalis, and then save cocoon quality from damage.
Preferably, the distance between mesh bag and refrigerator-freezer inner wall, mesh bag and mesh bag >=2 cm.In view of improving refrigerator-freezer profit With rate, preferred distance is 2-3 cm, to keep temperature between mesh bag uniform, and moisture recorder is placed in refrigerator-freezer, with reality When monitor refrigerator-freezer temperature conditions;
(4)Before silk cocoon use, it is taken out from refrigerator-freezer, be placed in 18-25 DEG C of room temperature environment defrosting 2-3 hours, solved Silk cocoon after jelly can be used directly.
The present invention has saved cocoon quality from damage while omitting cocoon drying process, simple for process, cheap, and can preferably protect The freshness for holding silkworm chrysalis improves the utility value of byproduct, can additionally play and be effectively protected unwinding, raising wire vent Rate, reduction myriametre hang rough effect, have preferable social and economic benefit.
Compared with prior art, the beneficial effects of the invention are as follows:
1)The present invention is easy to operate, easy to implement the method, and can preferably preserve cocoon quality, avoids and is preserved conventionally by cocoon drying process Silk cocoon red tape, reduces environmental pollution while energy saving;
2)The fresh cocoon almost and just purchased from silkworm raiser's hand of good pupa rate of the silk cocoon preserved using the store method of the present invention it is good Pupa rate indifference, can greatly improve the utility value of byproduct silkworm chrysalis;
3)Since store method does not need cocoon drying process, it is possible to reduce during cocoon drying, heating makes immature cocoon activity add The phenomenon that play destruction internal layer cocoon layer, occurs;
4)Silkworm cocoon reelability rate, length of non broken filament, the myriametre preserved using the store method of the present invention is hung the indexs such as rough and is above routine Dried cocoon filature, improve the yield and quality of raw silk.
Description of the drawings
Fig. 1 is the schematic diagram that cocoon basket of the present invention fills silk cocoon;
Fig. 2 is the schematic diagram in mesh bag of the present invention placement refrigerator-freezer;
Fig. 3 is the reelability percentage comparison diagram of the embodiment of the present invention and comparative example 1;
Fig. 4 is the length of non broken filament comparison diagram of the embodiment of the present invention and comparative example 1;
Fig. 5 is that the myriametre of the embodiment of the present invention and comparative example 1 hangs rough comparison diagram;
Fig. 6 is the good pupa rate comparison diagram of the embodiment of the present invention and comparative example 2;
Wherein, 1:Cocoon basket, 2:Silk cocoon, 3:Refrigerator-freezer, 4:Mesh bag.
Specific implementation mode
Below by embodiment, invention is further described in detail.
Embodiment 1
(1)It is 18 DEG C in temperature, in the environment that humidity is 60%, the fresh spring cocoon just purchased from peasant's hand is packed into six faces and is breathed freely Plastics cocoon basket 1 in, be designed as 3/5 or so of cocoon basket total capacity per the fresh cocoon amount of cocoon basket, the silk cocoon 2 in silk cocoon cocoon basket is at " recessed " word Shape(Such as Fig. 1), kicked after preserving 1 day in such circumstances except the spoiled cocoon for being unsatisfactory for requirement of experiment;
(2)Fresh cocoon is dispensed into hollow out mesh bag 4, each mesh bag fills fresh cocoon 200, accounts for the 2/3 of mesh bag total capacity;
(3)After silkworm pupates 3 days, the mesh bag for installing fresh cocoon layering is suspended in the refrigerator-freezer 3 that temperature is -20 DEG C or so side by side (Such as Fig. 2)It preserves, the distance between mesh bag and refrigerator-freezer inner wall, mesh bag and mesh bag are 2 cm, and temperature note is placed in refrigerator-freezer Instrument is recorded, to monitor the temperature conditions of refrigerator-freezer in real time;
(4)It preserves 10 days, it is taken out from refrigerator-freezer, is placed in 20 DEG C or so of room temperature environment after thawing 3 hours, with 200 Unwinding investigation experiment is carried out for an area, test reelability percentage, length of non broken filament and myriametre are hung rough;It separately takes 200 to do sectility experiment, calculates Good pupa rate, and observe the appearance character of silk cocoon.
Embodiment 2
(1)It is 20 DEG C in temperature, in the environment that humidity is 65%, the fresh spring cocoon just purchased from peasant's hand is packed into six faces and is breathed freely Bamboo cocoon basket 1 in, be designed as 2/5 or so of cocoon basket total capacity per the fresh cocoon amount of cocoon basket, the silk cocoon 2 in silk cocoon cocoon basket is at " recessed " word Shape(Such as Fig. 1), the spoiled cocoon for being unsatisfactory for requirement of experiment is rejected after preserving 3 days in such circumstances;
(2)Fresh cocoon is dispensed into hollow out mesh bag 4, each mesh bag fills fresh cocoon 100, accounts for the 1/3 of mesh bag total capacity;
(3)After silkworm pupates 5 days, the mesh bag for installing fresh cocoon layering is suspended in the refrigerator-freezer 3 that temperature is -15 DEG C or so side by side (Such as Fig. 2)It preserves, the distance between mesh bag and refrigerator-freezer inner wall, mesh bag and mesh bag are 2 cm, and temperature note is placed in refrigerator-freezer Instrument is recorded, to monitor the temperature conditions of refrigerator-freezer in real time;
(4)It preserves 25 days, it is taken out from refrigerator-freezer, is placed in 23 DEG C or so of room temperature environment after thawing 2 hours, with 200 Unwinding investigation experiment is carried out for an area, test reelability percentage, length of non broken filament and myriametre are hung rough;It separately takes 200 to do sectility experiment, calculates Good pupa rate, and observe the appearance character of silk cocoon.
Embodiment 3
(1)It is 20 DEG C in temperature, in the environment that humidity is 75%, the fresh spring cocoon just purchased from peasant's hand is packed into six faces and is breathed freely Plastics cocoon basket 1 in, be designed as 4/5 or so of cocoon basket total capacity per the fresh cocoon amount of cocoon basket, the silk cocoon 2 in silk cocoon cocoon basket is at " recessed " word Shape(Such as Fig. 1), the spoiled cocoon for being unsatisfactory for requirement of experiment is rejected after preserving 5 days in such circumstances;
(2)Fresh cocoon is dispensed into hollow out mesh bag 4, each mesh bag fills fresh cocoon 100, accounts for the 1/3 of mesh bag total capacity;
(3)After silkworm pupates 7 days, the mesh bag for installing fresh cocoon layering is suspended in the refrigerator-freezer 3 that temperature is -25 DEG C or so side by side (Such as Fig. 2)It preserves, the distance between mesh bag and refrigerator-freezer inner wall, mesh bag and mesh bag are 2 cm, and temperature note is placed in refrigerator-freezer Instrument is recorded, to monitor the temperature conditions of refrigerator-freezer in real time;
(4)It preserves 40 days, it is taken out from refrigerator-freezer, is placed in 25 DEG C or so of room temperature environment after thawing 2 hours, with 200 Unwinding investigation experiment is carried out for an area, test reelability percentage, length of non broken filament and myriametre are hung rough;It separately takes 200 to do sectility experiment, calculates Good pupa rate, and observe the appearance character of silk cocoon.
Embodiment 4
(1)It is 25 DEG C in temperature, in the environment that humidity is 65%, the fresh spring cocoon just purchased from peasant's hand is packed into six faces and is breathed freely Bamboo cocoon basket 1 in, be designed as 3/5 or so of cocoon basket total capacity per the fresh cocoon amount of cocoon basket, the silk cocoon 2 in silk cocoon cocoon basket is at " recessed " word Shape(Such as Fig. 1), the spoiled cocoon rejected be unsatisfactory for requirement of experiment after a week is preserved in such circumstances;
(2)Fresh cocoon is dispensed into hollow out mesh bag 4, each mesh bag fills fresh cocoon 100, accounts for the 1/3 of mesh bag total capacity;
(3)After silkworm pupates 9 days, the mesh bag for installing fresh cocoon layering is suspended in the refrigerator-freezer 3 that temperature is -20 DEG C or so side by side (Such as Fig. 2)It preserves, the distance between mesh bag and refrigerator-freezer inner wall, mesh bag and mesh bag are 2 cm, and temperature note is placed in refrigerator-freezer Instrument is recorded, to monitor the temperature conditions of refrigerator-freezer in real time;
(4)It preserves 60 days, it is taken out from refrigerator-freezer, is placed in 18 DEG C or so of room temperature environment after thawing 3 hours, with 200 Unwinding investigation experiment is carried out for an area, test reelability percentage, length of non broken filament and myriametre are hung rough;It separately takes 200 to do sectility experiment, calculates Good pupa rate, and observe the appearance character of silk cocoon.
Comparative example 1
The silk cocoon with the village mouthful is selected, carries out process of selecting silkworm after conventionally first drying silk cocoon again, equally with 200 for one Area carries out unwinding investigation experiment, and test reelability percentage, length of non broken filament and myriametre are hung rough.
Comparative example 2
The silk cocoon with the village mouthful is selected, directly takes 200 to do sectility experiment the silk cocoon just purchased from silkworm raiser's hand, and calculate pupa Rate.
Comparative example 3
It selects the silk cocoon with the village mouthful to be dispensed into silk cocoon in mesh bag with 200/bag after process of selecting silkworm, then silk cocoon will be housed Mesh bag be deposited in -20 DEG C of refrigerator-freezer and preserve 10 days successively, observe the appearance character of silk cocoon.
By above-mentioned experiment, can obtain to draw a conclusion:
The result of embodiment 1-4 and comparative example 1 is as shown in Figures 3 to 5, and the silk cocoon that the present invention preserves solves for baked cocoom The rate of relaxing, length of non broken filament improve, and myriametre hangs rough reduction.
The results are shown in Figure 6 for embodiment 1-4 and comparative example 2, and the silk cocoon preserved under the present invention is relative to rigidifying pupa silkworm For cocoon, good pupa rate difference very little can preferably keep the freshness of silkworm chrysalis.
Embodiment 1-4 and comparative example 3 it is found that stack the silk cocoon of preservation in a limited space, because hyther goes out successively Now the phenomenon that a small amount of cocoon layer jaundice, and the original state of fresh cocoon can be kept substantially according to the silk cocoon that the present invention preserves.

Claims (10)

1. a kind of fresh cocoon store method of sample, which is characterized in that the store method is following steps:
(1)The fresh cocoon of rigidifying pupa is packed into cocoon basket under temperature and humidity conditions,
(2)Fresh cocoon is dispensed into hollow out mesh bag, each mesh bag fills fresh cocoon 100-200;
(3)The mesh bag for installing fresh cocoon layering is suspended in refrigerator-freezer side by side before silkworm chrysalis moth;
(4)Before silk cocoon use, it is taken out from refrigerator-freezer, is placed in room temperature environment and is used after defrosting 2-3 hours.
2. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(1)In temperature be 18-25 DEG C, humidity 60-75%.
3. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(1)Described in cocoon Basket is the product that each face is breathed freely.
4. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(1)In fresh cocoon amount be The 2/5-4/5 of cocoon basket total capacity.
5. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(1)In be placed on cocoon Silk cocoon in basket was gradually decreased from four weeks to cocoon basket center, was arranged in concave.
6. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(1)It is middle by rigidifying pupa Fresh cocoon rejects the spoiled cocoon for being unsatisfactory for requirement of experiment in 7.
7. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(2)In fresh cocoon amount be The 1/3-2/3 of mesh bag total capacity.
8. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(3)In at least in silkworm The mesh bag for installing fresh cocoon layering is suspended in refrigerator-freezer side by side in 10 days after pupating.
9. the fresh cocoon store method of a kind of sample according to claim 1 or 8, which is characterized in that step(3)Middle refrigerator-freezer Temperature is -15 DEG C ~ -25 DEG C.
10. a kind of fresh cocoon store method of sample according to claim 1, which is characterized in that step(3)Middle mesh bag and ice The distance between cabinet inner wall, mesh bag and mesh bag >=2cm.
CN201810015294.3A 2018-01-08 2018-01-08 A kind of fresh cocoon store method of sample Pending CN108397951A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113897687A (en) * 2021-10-20 2022-01-07 浙江理工大学 High-additional-value raw silk processing method

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CN202378769U (en) * 2011-12-12 2012-08-15 昆明日晟农业科技有限公司 Special van for aphidius transportation
JP2015186457A (en) * 2014-03-27 2015-10-29 敬吾 竹内 Feed for wild silkworms, processing method therefor, method for raising wild silkworms and method for producing wild silkworm cocoon
CN104213200A (en) * 2014-08-26 2014-12-17 横县桂华茧丝绸有限责任公司 Method for reeling silk from mulberry silkworm fresh cocoons by virtue of cocoon cooking
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113897687A (en) * 2021-10-20 2022-01-07 浙江理工大学 High-additional-value raw silk processing method
CN113897687B (en) * 2021-10-20 2023-05-19 浙江理工大学 Processing method of raw silk with high added value

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Application publication date: 20180814