CN113243358A - Method for storing tussah eggs - Google Patents
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- CN113243358A CN113243358A CN202110276081.8A CN202110276081A CN113243358A CN 113243358 A CN113243358 A CN 113243358A CN 202110276081 A CN202110276081 A CN 202110276081A CN 113243358 A CN113243358 A CN 113243358A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000003860 storage Methods 0.000 claims abstract description 25
- 238000007789 sealing Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 229960002233 benzalkonium bromide Drugs 0.000 claims description 9
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 241000255978 Antheraea pernyi Species 0.000 claims description 3
- 241000255789 Bombyx mori Species 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052782 aluminium Inorganic materials 0.000 claims description 3
- 239000011888 foil Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000007667 floating Methods 0.000 abstract description 13
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- 238000005057 refrigeration Methods 0.000 abstract description 7
- 230000003071 parasitic effect Effects 0.000 description 19
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- 238000012360 testing method Methods 0.000 description 10
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- 210000004681 ovum Anatomy 0.000 description 5
- 241000258937 Hemiptera Species 0.000 description 4
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses a method for storing tussah eggs, which comprises the following steps: sealing tussah egg, soaking in liquid medium, and refrigerating at 0-3 deg.C. The liquid medium may be water. According to the method for storing the tussah eggs, the tussah eggs are stored for 180 days under the water bath condition of 0-3 ℃, and the egg floating rate, the green egg rate, the egg loss rate, the emergence rate and the female rate have no obvious difference from CK. The storage method provided by the invention obviously prolongs the effective storage time of the tussah eggs, reduces the loss of refrigeration to the tussah eggs, and reduces the production cost of commercialized production of vespid.
Description
Technical Field
The invention relates to the technical field of biological control of crops, in particular to a method for storing tussah eggs.
Background
The Applegia pleioides (Antatatus fullloides) belongs to hymenoptera and Convolvulaceae, is the dominant natural enemy of fruit tree stinkbug pests, is currently produced industrially on a large scale in southern China, and is a main bee species successfully applied to field control of litchi stinkbug. The technology is one of the main biological control means for litchi chinensis bugs in litchi and longan production areas such as Guangdong, Fujian, Guangxi, Hainan and the like at present, only 2017 and 2019 years prove that the demonstration application in Guangdong Guangzhou, Huizhou, Caragana, Dongguan, Shenzhen, Zhuhai, Guangxi Yulin, North sea, Hainan Haikou, Mitsui, Mitsuma, Fujian, Siluchuan and the like exceeds 1 ten thousand mu, and the effect of controlling the litchi bugs by using the Apis cerana reaches more than 80 percent. The P.areola is also used for controlling tea-wing bugs and other local heaven bugs in Beijing, Western-Ann, hong Kong, Taiwan, Thailand, USA, Europe and Australia, etc.
The tussah egg is the most important artificial propagation intermediate host in commercial production of the verruca plana, and has the advantages of large egg size, high bee breeding efficiency, rich resources, low price, convenience in transportation and the like. Due to the seasonality of the target pests, a large amount of vespid needs to be accumulated for release before the peak egg laying period of the pests. The state that fresh host eggs and bee seeds meet in time cannot be guaranteed in the production process of the vespidae, and the intermediate host tussah eggs need to be preserved for guaranteeing production. And part of parasitic wasps are not cold-resistant and can only prolong the shelf life by refrigerating host eggs. Due to different preservation modes, the quality of the tussah eggs, the adaptability of the vespidae, the production cost and the like are directly influenced, so that the large-scale breeding and utilization of the vespidae are restricted.
At present, the storage method of the tussah eggs is mainly a preservation mode of refrigerating female tussah moths, the method can only perform laparotomy on the female moths when needed, the operation is complex, the preservation time is not longer than 10 days, and the requirement of scale production of the vespidae cannot be met.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a method for storing the tussah eggs, which obviously prolongs the effective storage time of the tussah eggs.
The purpose of the invention is realized by the following technical scheme:
a method for storing tussah eggs comprises the following steps: sealing tussah egg, soaking in liquid medium, and refrigerating at 0-3 deg.C.
Preferably, the refrigeration preservation is carried out at 0-3 ℃, and specifically comprises the following steps:
and (3) refrigerating and storing under the conditions that the temperature is 0-3 ℃ and the relative humidity is 65-75%.
Preferably, the liquid medium is water.
Preferably, the tussah eggs are sealed, and the method specifically comprises the following steps:
the tussah eggs are put into a self-sealing bag or an aluminum foil bag and then sealed.
Preferably, before the tussah eggs are sealed, the following steps are also carried out:
sterilizing Antheraea pernyi eggs with benzalkonium bromide solution, and air drying.
Preferably, the volume concentration of the benzalkonium bromide solution is 0.1-0.2%.
Preferably, the sterilization time is 5 to 10 minutes.
Preferably, the time for refrigerated storage is 6 months.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the egg floating rate of the tussah eggs refrigerated for 180 days by the storage method of the invention has no obvious difference with that of fresh tussah eggs (refrigerated for 0 d); the green egg rate has no obvious difference with the fresh tussah eggs; the egg loss rate is not obviously different from that of the fresh tussah eggs; the parasitic rate is not obviously different from that of the fresh tussah eggs; the eclosion rate is not obviously different from that of fresh tussah eggs; the effective eclosion rate was 53.33%; the female rate of the vespid is not obviously different from that of the fresh tussah eggs. According to the invention, the tussah eggs are immersed in the liquid medium for refrigeration storage at 0-3 ℃, so that the effective storage time of the tussah eggs is obviously prolonged, the loss of refrigeration to the tussah eggs is reduced, and the production cost of commercialized production of the vespidae is reduced.
Drawings
FIG. 1 is a graph showing the variation of egg floating rate of tussah eggs stored in different storage modes for different time periods.
FIG. 2 is a graph showing the change of the rate of eggs stored in different storage modes for different time.
FIG. 3 is a graph showing the change of egg loss rate of tussah eggs stored for different time periods in different storage modes.
Fig. 4 is a graph showing the variation of parasitic rate of the vespid wasps stored in different storage modes for different time periods.
Fig. 5 is a graph showing the variation of the emergence rate of the plane-belly bees of the tussah eggs stored for different time periods in different storage modes.
FIG. 6 is a graph showing the variation of effective emergence rate of tussah eggs stored in different storage modes for different time periods.
Fig. 7 is a bar graph of the female rate of vespid wasps stored in different storage modes for different time periods.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
The storage method of the tussah eggs in the embodiment includes sterilizing the tussah eggs with benzalkonium bromide solution with volume concentration of 0.15% for 5min, then drying the eggs in the air, then packaging the eggs at a rate of 250 g/bag, sealing the eggs with a self-sealing bag, and then performing the following treatment: a: placing in a bucket, pressing with stone, submerging below water surface, and refrigerating in a refrigerator with 0-3 deg.C and 70% relative humidity.
In order to illustrate the storage method of the tussah eggs in the embodiment of the present invention, three groups of comparative samples are set in the embodiment, and the specific experimental procedures and test results are as follows:
1 materials and methods
1.1 materials
Parasitic wasp for test: the Mucuna cinquefoil (Anastatus fulloi) is obtained from an egg block of a lygus sinensis collected in a litchi garden of a dolostomia of the Guangdong academy of agricultural sciences, and a population is established by breeding for multiple generations in a room with tussah eggs as a substitute host. The bee species were identified by Penlingfei, institute of plant protection, university of agriculture and forestry, Fujian.
Test hosts: the tussah ovum is cesarean ovum of female tussah moth supplied in Liaoning area. The preparation method comprises the following steps: taking the female tussah silkworm moth which is primarily eclosion and completely spreads wings, cutting open the abdomen to take eggs, repeatedly cleaning with clear water, placing at room temperature for airing, and standing for later use after the eggs are dried.
Test egg cards and instruments: the specification of the Apis veolens egg card is 7cm multiplied by 6.5cm, each card has about 500 eggs, and the card is self-made by plant protection research institute of agricultural academy of sciences of Guangdong province; a hai ice chest.
1.2 methods
1.2.1 tussah egg refrigeration treatment
Sterilizing tussah eggs by using benzalkonium bromide solution with volume concentration of 0.15% for 5min, drying, then packaging at a rate of 250 g/bag, packaging and sealing by using a self-sealing bag, and then carrying out the following treatment: a: placing in a bucket, pressing with stone, submerging below water surface, placing the bucket in a 0-3 deg.C refrigerator with relative humidity of 70% for refrigerating; b: refrigerating in 0-3 deg.C refrigerator; c: refrigerating in a freezer at-5 deg.C; d: refrigerating in a freezer at-18 deg.C; each treatment was at least 3 replicates. After being refrigerated for 0d (CK), 3d, 21d, 28d, 42d, 70d, 90d, 150d and 180d, the samples are taken out for subsequent tests.
1.2.2 Effect of Low temperature refrigeration on Oak egg floating rate
Taking out 500 eggs from each treatment, placing in clear water, standing for 2min, examining the number of floated eggs, and repeating for at least 3 times. The settled eggs are collected and used for the subsequent Anastatus ventricosa breeding test.
1.2.3 influence of Low-temperature refrigeration on the rate of blue eggs of tussah eggs and adaptability of verruca plana
Sterilizing the above-mentioned sinking Antheraea pernyi egg granules with 0.15% benzalkonium bromide solution for 5min, air drying (preferably making egg surface become egg white), and sticking it on 1/4 Apis mellifera egg card with white latex adhesive, about 250 granules. Placing 1/2 egg cards in a plastic space cup, selecting 10 female bees and 2 male bees within 24h of emergence, inoculating, culturing in a growth room at 26 deg.C with RH 70% and 16L:8D, and sealing the space cup with gauze. Each treatment was repeated 3 times. And (4) after the newly parasitic eggs are hatched out, investigating the number of feathers, the number of non-parasitic eggs and the number of green eggs. And selecting refrigerated treatments of 0d, 3d, 28d, 70d, 150d and 180d for carrying out adaptability analysis on the vervain. The green egg means that the verruca plana cannot complete development, is an injured or immature egg, and finally appears cyan.
1.3 statistical analysis of data
Data statistics One-way ANOVA in SPSS statistics software was used for analysis of variance and Tamhane was used for the significance of differences test.
The floating egg rate is equal to the floating egg/(floating egg + sinking egg)
Green egg rate ═ green egg/(parasitic egg + green egg + parasitic)
The egg loss rate is (green egg + floating egg)/(parasitic egg + green egg + floating egg + not parasitic)
The parasitic rate is the parasitic ovum/(parasitic ovum + not parasitic ovum)
Eclosion rate (eclosion number/parasitic egg)
Effective eclosion rate (eclosion number/(parasitic egg + green egg + floating egg + non-parasitic egg)
Female rate is the number of female bees/number of female bees + number of male bees
2 results
The test results of this example are shown in FIGS. 1-7. It can be known that the egg floating rate of the tussah eggs is not obviously different from CK after the tussah eggs are stored for 180 days under the water bath condition of 0-3 ℃; the green egg rate is not obviously different from CK; the egg loss rate is not obviously different from CK; the parasitic rate of vespid shows a downward trend, but there was no significant difference between storage 180d and CK; the emergence rate is not obviously different from CK; the effective emergence rate is 53.33%, although the effective emergence rate (64.85%) for the propagation of vespid beetles is reduced compared with that of the unhardened tussah eggs, but is significantly higher than that of the samples of other groups; the female rate of the vespid has no significant difference with CK.
Example 2
The storage method of the tussah eggs in the embodiment is to sterilize the tussah eggs with benzalkonium bromide solution with the volume concentration of 0.1% for 7min, dry the eggs in the air, package the eggs in a bag with the volume concentration of 250 g/bag, package and seal the eggs with aluminum foil bags, and then perform the following treatment: a: placing in a bucket, pressing with stone, submerging below water surface, and refrigerating in a refrigerator with 0-3 deg.C and relative humidity of 65%.
The test results of this example show that: after the tussah eggs are stored for 180 days under the water bath condition of 0-3 ℃, the egg floating rate is not obviously different from CK; the green egg rate is not obviously different from CK; the egg loss rate is not obviously different from CK; the parasitic rate of vespid shows a downward trend, but there was no significant difference between storage 180d and CK; the emergence rate is not obviously different from CK; the effective emergence rate of the tussah eggs for the propagation of the vespidae is slightly reduced relative to the effective emergence rate of the non-refrigerated tussah eggs for the propagation of the vespidae; the female rate of the vespid has no significant difference with CK.
Example 3
The storage method of the tussah eggs in the embodiment includes sterilizing the tussah eggs with benzalkonium bromide solution with volume concentration of 0.2% for 10min, then drying the eggs in the air, then packaging the eggs at a rate of 250 g/bag, sealing the eggs with a self-sealing bag, and then performing the following treatment: a: placing in a bucket, pressing with stone, submerging below water surface, and refrigerating in a refrigerator with 0-3 deg.C and 75% relative humidity.
The test results of this example show that: after the tussah eggs are stored for 180 days under the water bath condition of 0-3 ℃, the egg floating rate is not obviously different from CK; the green egg rate is not obviously different from CK; the egg loss rate is not obviously different from CK; the parasitic rate of vespid shows a downward trend, but there was no significant difference between storage 180d and CK; the emergence rate is not obviously different from CK; the effective emergence rate of the tussah eggs for the propagation of the vespidae is slightly reduced relative to the effective emergence rate of the non-refrigerated tussah eggs for the propagation of the vespidae; the female rate of the vespid has no significant difference with CK.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (8)
1. A method for storing tussah eggs is characterized by comprising the following steps: sealing tussah egg, soaking in liquid medium, and refrigerating at 0-3 deg.C.
2. The tussah egg storage method according to claim 1, wherein the cold storage is performed at 0-3 ℃, specifically:
and (3) refrigerating and storing under the conditions that the temperature is 0-3 ℃ and the relative humidity is 65-75%.
3. The method for storing tussah eggs of claim 1, wherein the liquid medium is water.
4. The method for storing the tussah eggs, according to claim 1, wherein the tussah eggs are sealed, specifically:
the tussah eggs are put into a self-sealing bag or an aluminum foil bag and then sealed.
5. The method for storing the eggs of tussah silkworms of claim 1, wherein the method further comprises the following steps before sealing the eggs of tussah silkworms:
sterilizing Antheraea pernyi eggs with benzalkonium bromide solution, and air drying.
6. The method for storing the tussah eggs, according to claim 5, wherein the volume concentration of the benzalkonium bromide solution is 0.1-0.2%.
7. The method for storing the tussah eggs, according to claim 5, wherein the time for sterilization is 5-10 minutes.
8. The method for storing tussah eggs as claimed in claim 1, wherein the storage period is 6 months.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110276081.8A CN113243358B (en) | 2021-03-15 | 2021-03-15 | Method for storing tussah eggs |
| ZA2022/00157A ZA202200157B (en) | 2021-03-15 | 2022-01-03 | Storage method for antheraea pernyi eggs |
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| CN202110276081.8A CN113243358B (en) | 2021-03-15 | 2021-03-15 | Method for storing tussah eggs |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023274430A3 (en) * | 2022-01-18 | 2023-02-23 | 安徽科技学院 | Method for prolonging storage period of egg masses of telenomus remus nixon |
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| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023274430A3 (en) * | 2022-01-18 | 2023-02-23 | 安徽科技学院 | Method for prolonging storage period of egg masses of telenomus remus nixon |
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| CN113243358B (en) | 2022-05-24 |
| ZA202200157B (en) | 2022-03-30 |
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Application publication date: 20210813 Assignee: GUANGZHOU YUEFENG BIOLOGICAL CONTROL TECHNOLOGY Co.,Ltd. Assignor: PLANT PROTECTION Research Institute GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022440000119 Denomination of invention: A kind of storage method of tussah eggs Granted publication date: 20220524 License type: Common License Record date: 20220907 Application publication date: 20210813 Assignee: Huizhou yimite Technology Co.,Ltd. Assignor: PLANT PROTECTION Research Institute GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022440000111 Denomination of invention: A kind of storage method of tussah eggs Granted publication date: 20220524 License type: Common License Record date: 20220906 |