CN108395319A - A kind of complex microorganism liquid fertilizer - Google Patents
A kind of complex microorganism liquid fertilizer Download PDFInfo
- Publication number
- CN108395319A CN108395319A CN201810184377.5A CN201810184377A CN108395319A CN 108395319 A CN108395319 A CN 108395319A CN 201810184377 A CN201810184377 A CN 201810184377A CN 108395319 A CN108395319 A CN 108395319A
- Authority
- CN
- China
- Prior art keywords
- parts
- fertilizer
- liquid fertilizer
- complex microorganism
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of fertilizer, and in particular to a kind of complex microorganism liquid fertilizer.The complex microorganism liquid fertilizer of the present invention includes microbial inoculum, organic-biological nitrogen, inorganic fertilizer, middle amount inorganic fertilizer and bioactivity organic matter.Complex microorganism liquid fertilizer provided by the invention provides required inorganic nutrients for plant growth, but is that of avoiding inorganic nutrients to the killing beneficial to viable bacteria in fertilizer, extends to the viable bacteria time-to-live.
Description
Technical field
The present invention relates to a kind of fertilizer, and in particular to a kind of complex microorganism liquid fertilizer.
Background technology
China's controlling disease substantially uses chemical pesticide at present, and chemical pesticide control is with its strong toxicity, control effect
It is good, at low cost and generally welcome by peasant.China's pesticide annual consumption is ten thousand tons of 80-100, is occupied first place in the world.But a large amount of uses
Chemical pesticide, second is that bringing Practice for Pesticide Residue in Agricultural Products problem, causes human health first, causing pollution by pesticides to environment
It threatens.To reduce the use of chemical pesticide, cultural control in recent years, biological green prevention are at home and abroad gradually risen.
Exploitation biological pesticide and microbial-bacterial fertilizer have become a kind of international trend.Existing liquid microbe fertilizer
Material, generally contains only viable bacteria body and organic nutrient, there is no inorganic nutrients so that available nutrient of fertilizer is single, and nutrition is not
Comprehensively, fertilizer efficiency is undesirable.But in the case where being not added with microbiological antiseptic, a certain amount of nothing is such as added into liquid microbe bacterium solution
Machine nutrients of nitrogen, phosphorus, potassium, especially nitrogen have very strong lethality to the viable bacteria in liquid, influence viable bacteria survival, shorten Liquid Fertilizer
The storage life of material.
Therefore there is an urgent need to a kind of nutrition currently on the market comprehensive, the longer liquid microbe fertilizer of storage life.
Invention content
The object of the present invention is to provide a kind of complex microorganism liquid fertilizers, to solve problems of the prior art.
The purpose of the present invention is what is be achieved through the following technical solutions：A kind of complex microorganism liquid fertilizer, including micro- life
Object bacterium solution, organic-biological nitrogen, inorganic fertilizer, middle amount inorganic fertilizer and bioactivity organic matter.
Further, the complex microorganism liquid fertilizer includes according to mass parts meter：6~15 parts of microbial inoculum, has
5~15 parts of machine biological nitrogen, 10~30 parts of inorganic fertilizer, 3~5 parts of middle amount inorganic fertilizer and bioactivity organic matter 15~40
Part.
Further, the complex microorganism liquid fertilizer includes according to mass parts meter：8~14 parts of microbial inoculum, has
7~13 parts of machine biological nitrogen, 15~23 parts of inorganic fertilizer, 3.5~4.5 parts of middle amount inorganic fertilizer and bioactivity organic matter 18~
30 parts.
Further, the complex microorganism liquid fertilizer includes according to mass parts meter：12 parts of microbial inoculum, You Jisheng
20 parts of 11 parts of object nitrogen, 20 parts of inorganic fertilizer, 4 parts of middle amount inorganic fertilizer and bioactivity organic matter.
Further, the organic-biological nitrogen it is any in amino acid, polypeptide, protein, Fish protein, liquid molasses or
It is a variety of, preferably amino acid.
Further, the inorganic fertilizer is selected from ammonium sulfate, ammonium chloride, ammonium nitrate, sodium nitrate, calcium nitrate, urea, chlorination
Appoint one or more in potassium, potassium sulfate and potassium nitrate, preferably potassium nitrate, urea, ammonium nitrate.
Further, the middle amount inorganic fertilizer is selected from calcium chloride, calcium nitrate, amino acid chelated calcium, ethylenediamine tetra-acetic acid
It chelates in calcium, magnesium chloride, magnesium sulfate and magnesium nitrate and appoints one or more, preferably magnesium nitrate, amino acid chelated calcium and ethylenediamine
Tetraacethyl chelates calcium.
Further, any in amino acid, polypeptide, protein, Fish protein, liquid molasses of bioactivity organic matter or
It is a variety of.
Further, the preparation method of the microbial inoculum includes step：Culture medium is separately added into the first tank and
It in two tanks, is closed, is sterilized；After the completion of sterilizing, respectively cool down to it processing, ensure in temperature-fall period tank pressure for 0.07~
0.14Mpa；When temperature drops to 28~36 DEG C, the first tank of constant temperature and pressure pair is inoculated with；Ferment 9~12h, by the fermentation of the first tank
Liquid is added to the second tank relaying supervention ferment, and control condition puts tank to get microorganism with the first tank when thalline forms 90~95%
Bacterium solution.
Further, the bacterium of inoculation is selected from bacillus subtilis, bacillus licheniformis, bacillus megaterium, glue fat bud
Spore bacillus appoints one or more with Bacillus cercus.
The number of calcium is 1~3 part in the microbial liquid fertilizer, and the number containing magnesium is 1~3 part.
The present invention has the following advantages that compared with prior art：
Complex microorganism liquid fertilizer provided by the invention provides required inorganic nutrients for plant growth, but is that of avoiding
Inorganic nutrients extend to the viable bacteria time-to-live killing beneficial to viable bacteria in fertilizer.
Crop resistance, and the absorption to fertilizer and utilization rate can be improved, keep plant growth healthy and strong, also can in turn
Promote the breeding of biocontrol microorganisms to improve crop disease-resistant ability, and contributes to form good agricultural eco-system.
Pesticide dosage can be reduced while reducing fertilizer amount, pesticide residue and pollution are reduced, for sustainable agriculture
The raising of development and people's living standard with pollution-free food production plays an important roll.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is a part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The complex microorganism liquid fertilizer of the present invention, including microbial inoculum, organic-biological nitrogen, inorganic fertilizer, middle amount
Inorganic fertilizer and bioactivity organic matter.The organic-biological nitrogen is selected from amino acid, polypeptide, protein, Fish protein, liquid molasses
In appoint one or more, preferably amino acid.The inorganic fertilizer be selected from ammonium sulfate, ammonium chloride, ammonium nitrate, sodium nitrate, calcium nitrate,
Appoint one or more in urea, potassium chloride, potassium sulfate and potassium nitrate, preferably potassium nitrate, urea, ammonium nitrate.The middle amount without
Machine fertilizer is selected from calcium chloride, calcium nitrate, amino acid chelated calcium, ethylenediamine tetra-acetic acid chelating calcium, magnesium chloride, magnesium sulfate and magnesium nitrate
In appoint one or more, preferably magnesium nitrate, amino acid chelated calcium and ethylenediamine tetra-acetic acid chelate calcium.Bioactivity organic matter selects
Appoint one or more from amino acid, polypeptide, protein, Fish protein, liquid molasses.
The complex microorganism liquid fertilizer includes according to mass parts meter：6~15 parts of microbial inoculum, organic-biological nitrogen
5~15 parts, 10~30 parts of inorganic fertilizer, 15~40 parts of 3~5 parts of middle amount inorganic fertilizer and bioactivity organic matter.Preferably, it presses
Include according to mass parts meter：8~14 parts of microbial inoculum, 7~13 parts of organic-biological nitrogen, 15~23 parts of inorganic fertilizer, middle amount without
18~30 parts of 3.5~4.5 parts of machine fertilizer and bioactivity organic matter.It is highly preferred that the complex microorganism liquid fertilizer according to
Mass parts meter includes：12 parts of microbial inoculum, 11 parts of organic-biological nitrogen, 20 parts of inorganic fertilizer, 4 parts of middle amount inorganic fertilizer and life
20 parts of object active organic matter.
The preparation method of the microbial inoculum includes step：Culture medium is separately added into the first tank and the second tank, it will
It is closed, sterilizing；After the completion of sterilizing, respectively cool down to it processing, ensures that tank pressure is 0.07~0.14Mpa in temperature-fall period；
When temperature drops to 28~36 DEG C, the first tank of constant temperature and pressure pair is inoculated with；Ferment 9~12h, and the zymotic fluid of the first tank is added to
Second tank relays supervention ferment, and control condition puts tank to get microbial inoculum with the first tank when thalline forms 90~95%.Its
In, the first tank is seed fermenter, and the second tank is fermentation tank；The bacterium of inoculation be selected from bacillus subtilis, bacillus licheniformis,
Appoint one or more in bacillus megaterium, glue fat bacillus and Bacillus cercus.Calcium in the microbial liquid fertilizer
Number is 1~3 part, and the number containing magnesium is 1~3 part.
Bacillus subtilis (Bacillus subtills) hinders in such a way that competitive growth breeding occupies living space
The growth of plant pathogenic fungi is stopped, a floor height privacy protection film can be quickly formed in plant surface, phytopathogen is made to cannot get
Living space, to protect plant to be endangered from disease fungus.In addition bacillus subtilis can secrete antibacterial substance, inhibit disease
Bacterium spore-germination and mycelia growth, to achieve the purpose that prevention and treatment.Simultaneously can activating soil nutrient, generate various plants
Hormone needed for growth and development.
Inorganic fertilizer is the nutrient that growth and development of plants must absorb, and on the one hand can accelerate plant growth, is especially added
The nutritional deficiency of plant growth appearance can be supplemented by entering moderate-element, increase plant products and enhancing plant cold resistance, disease-resistant, energy resistant to lodging
Power；On the other hand the elements such as nitrogen, phosphorus, potassium are provided for the growth and breeding of beneficial bacteria in bacterium solution, enhances thalline mobility and effect.
Bioactivity organic matter not only provides nutrition in liquid-phase system for microorganism, and soil can be increased by being administered to field
Organic matter improves soil aggregate structure；It is acted on through microbial metabolism, is converted into antibacterial substance, inhibit germ spore-germination and bacterium
Silk growth, conversion generate various plants hormone needed for growth and development.
Embodiment 1
300 and 3000L water is added in seed fermenter and fermentation tank respectively, then by bacillus subtilis bacterium culture medium (1L
20g glucose, 15g peptones, 5g sodium chloride, 0.5g beef extracts are added in water) it is separately added into seed fermenter and fermentation tank.
Seed fermenter and fermentation tank are closed, stirring slurry is opened, is being stirred continuously, with steam sterilizing, 121 DEG C of constant temperature 30min,
The thoroughly sterilizing simultaneously of the pipe fittings such as pipeline valve；After the completion of sterilizing, cooling processing is carried out to seed fermenter and fermentation tank, is being cooled down
It constantly is passed through purified compressed air to seed fermenter and fermentation tank in the process, ensures that tank pressure is 0.07~0.14Mpa.When
Temperature drops to 28~36 DEG C, and bacillus subtilis seed is taken fermentation plant, is inoculated with to seed fermenter, inoculum concentration is
1: 10, it ferments after connecing bacterium, temperature control is at 28~36 DEG C, and the voltage-controlled system of tank is in 0.07~0.14Mpa.When seed fermenter is sent out
The zymotic fluid of seed fermenter when fermentation reaches logarithmic phase, is added to fermentation tank and relays supervention ferment, control condition by 9~12h of ferment
Same seed fermenter puts tank when bacillus subtilis thalline sporulation 90~95% to get to microbial inoculum.Wherein send out
Bacterium solution viable count is more than 10,000,000,000/milliliter after ferment.
14 parts of microbial inoculum, 12 parts of urea, 9 parts of potassium nitrate, 1 part of amino acid 1,2 parts of amino acid calcium, 2 parts of magnesium nitrate, sugar
20 parts of sweet liquid.It is mixed, it is canned after being completely dissolved uniformly.Liquid fertilizer after canned should be sealed, avoid with
External environment contacts.
The viable count that primary fermentation liquid is mixed in the present embodiment is detected as 6.3 hundred million/milliliter, and above-mentioned various substances are added
Be mixed it is canned after, sampling Detection viable count be 5.37 hundred million/milliliter, after 5 days detect viable count be 5.70 hundred million/milliliter.
Embodiment 2
Microbial inoculum is prepared by 1 method of embodiment；
12 parts of bacillus subtilis bacterium solution, 14 parts of urea, 10 parts of potassium nitrate, 0 part of amino acid 1, EDTA-Ca4 parts, magnesium nitrate
2 parts, 18 parts of liquid molasses.
It mixed, stirred, canned by same procedure in embodiment 1.
The viable count that primary fermentation liquid is mixed in the present embodiment is detected as 6.8 hundred million/milliliter, and above-mentioned various substances are added
Be mixed it is canned after, sampling Detection viable count be 5.87 hundred million/milliliter, after 6 days detect viable count be 6.37 hundred million/milliliter.
Embodiment 3
Prepare microbial inoculum：By 10-50 parts of colistine sulfate, 1-2 parts of natamycin, 3-40 parts of l-Alanine, figured silk fabrics
8-50 parts of propylhomoserin, 50-200 parts of yeast extract, 50-200 parts of peptone, 1-10 parts of disodium hydrogen phosphate, 1-10 parts of sodium chloride, grape
20-200 parts sugared, 20-50 parts of fructose, 20-200 parts of brown sugar is placed and is stirred 20-30 minutes in sealing agitator tank, is uniformly mixed, then
After being stirred evenly in 50-200 parts of addition agitator tanks of Paenibacillus polymyxa bacterium powder, water is supplied again after 30 minutes, to reach more
The ratio of viscous series bacillus medicine fertilizer and water is 1：10 to 1：30 ratio starts to cultivate, first 12 hours after adjusting PH to 8-8.2
Minute ventilation volume liquid-gas ratio is 1：Ventilatory capacity is 1 after 0.6,12 hour：1, cultivation temperature is 20-35 DEG C, is cultivated 16-24 hours
Afterwards to get to the how viscous class gemma bar microbial inoculum of fermentation；
6 parts of microbial inoculum, 5 parts of amino acid 1,8 parts of ammonium sulfate, 2 parts of sodium nitrate, 3 parts of magnesium nitrate are organic with bioactivity
40 parts of matter.
Embodiment 4
15 parts of microbial inoculum, 5 parts of polypeptide, 15 parts of ammonium chloride, 15 parts of calcium nitrate, 5 parts of amino acid chelated calcium are lived with biology
15 parts of organic matter of property.
Embodiment 5
8 parts of microbial inoculum, 13 parts of protein, 5 parts of ammonium nitrate, 10 parts of urea, EDTA-Ca3.5 parts have with bioactivity
30 parts of machine matter.
Embodiment 6
14 parts of microbial inoculum, 7 parts of Fish protein, 12 parts of ammonium sulfate, 11 parts of potassium chloride, 2 parts of calcium chloride, 2.5 parts of magnesium nitrate
With 18 parts of bioactivity organic matter.
Embodiment 7
12 parts of microbial inoculum, 11 parts of liquid molasses, 8 parts of ammonium chloride, 12 parts of potassium sulfate, 1 part of calcium nitrate, magnesium sulfate 3 and life
20 parts of object active organic matter.
Embodiment 8
14.5 parts of microbial inoculum, 3 parts of amino acid, 3 parts of polypeptide, 25 parts of urea, 3.2 parts of amino acid chelated calcium are lived with biology
17 parts of organic matter of property.
Embodiment 9
7 parts of microbial inoculum, 8 parts of protein, 6 parts of liquid molasses, 3 parts of potassium nitrate, 10 parts of urea, EDTA-Ca1.3 parts, chlorine
Change 35 parts of 3.5 parts of magnesium and bioactivity organic matter.
The property of liquid fertilizer prepared by 1 embodiment 1-9 of table
| Living bacteria count | Organic matter | Inanimate matter | pH | Bacteriostasis rate | |
| Embodiment 1 | 5.70 hundred million/milliliter | 20.0% | 15.2% | 6.6 | 85.7% |
| Embodiment 2 | 6.37 hundred million/milliliter | 19.3% | 12.4% | 6.8 | 87.9% |
| Embodiment 3 | 4.15 hundred million/milliliter | 23.1% | 20.5% | 5.9 | 83.6% |
| Embodiment 4 | 5.63 hundred million/milliliter | 15.8% | 15.1% | 6.2 | 88.6% |
| Embodiment 5 | 4.30 hundred million/milliliter | 22.4% | 17.6% | 5.7 | 84.8% |
| Embodiment 6 | 5.80 hundred million/milliliter | 17.5% | 13.4% | 6.3 | 84.5% |
| Embodiment 7 | 6.20 hundred million/milliliter | 16.7% | 13.2% | 6.5 | 85.8% |
| Embodiment 8 | 5.93 hundred million/milliliter | 19.5% | 14.3% | 6.3 | 86.2% |
| Embodiment 9 | 5.42 hundred million/milliliter | 22.7% | 15.6% | 6.1 | 84.9% |
By doing ageing stability Observe and measure 3 months to above-mentioned 9 examples, detect living bacteria count, bacterium within 6 months
Number attenuation rate is proportionate with inorganic salt content substantially, and inorganic salinity pair can be reduced by adjusting proper content inorganic nutrients
The killing of microorganism can also provide the time-to-live that microorganism nutrient extends viable bacteria.
The viable bacteria survival condition of fertilizer prepared by 2 embodiment 1-9 of table
The powdery mildew field trial of 1 strawberry of test example
Microbial liquid fertilizer made from the above embodiment of the present invention 1,25% triazolone WP, commercial liquid fertilizer (Jiangsu
High nitrogen and potassium suspension type liquid fertilizer).
1, experimental design：
By field trial, the microbial liquid fertilizer, 25% triazolone WP and commercial liquid fertilizer are grown in strawberry
Phase to the specifically used effect of powdery mildew.
2, materials and methods
2.1 test site
It is tested September in 2017 on December 11st, 26 days 1, there is the Tang king for improving drip irrigation system in Jinan City Zhangqiu
The strawberry greenhouse of town 13 carries out.
Chance value autumn and winter alternating phases in crop growth period, temperature are between 0-10 DEG C, and day and night temperature is larger, to making
Object growth effect is larger.
2.2 soil overviews
Soil property is easily arid based on sandy loam, good permeability.
2.3 for studying object
Strawberry is planted in annual new transplanting.
2.4 test method
Strawberry growing way is more uniform, is in harvest initial stage, there is powdery mildew of strawberry infection.When testing initial administration, strawberry white powder
Disease is initial, and fragmentary blade is slightly fallen ill.Each experimental plot fertilizer and water management is consistent.Test block is set up respectively in No. 13 greenhouses, is tested
Cell random alignment, plot area 25m2, is repeated 4 times, and protection row is set between each processing, and experiment sets microbial liquid fertilizer and applies
Dose be 75,150,225kg/hm23 gradient, 25% triazolone WP formulation rates of comparison medicament be 675g/hm2, blank control
(CK) commercial liquid fertilizer is sprayed.Medicament is routinely sprayed.Uniformly spray leave dual sides, continuous dispenser 3 times, per minor tick 15d. examinations
Other chemical control diseases are not applied during testing；Fertilizer and microbial liquid fertilizer facility trickle irrigation.
2.5 investigation and calculating
Before dispenser, after first time medicine after 7d, second of medicine after 7d and third time medicine 7d totally 3 investigation strawberries morbidity feelings
Condition uses diagonal line five point sampling, every whole blades of 3 plants of investigation every plant of investigation, every leaf to be accounted for by lesion area per cell
The percentage classification records investigation total number of sheets of leaf area and sick number of sheets grade scales at different levels are as follows：0 grade of disease-free spot：1 grade, disease
Spot area accounts for 5% or less entire leaf area；3 grades, lesion area accounts for the 6%~15% of entire leaf area；5 grades, lesion area
Account for the 16%~25% of entire leaf area：7 grades, lesion area accounts for 26%~5O% of entire leaf area：9 grades, lesion area accounts for
Disease index and control effect is calculated as follows in 50% or more of entire leaf area：
3. result and analysis
The mixing of table 3 mixes liquid and prevents powdery mildew of strawberry effect
7d microbial liquids fertilizer 75 after first time dispenser, 150, the average disease index of 225kg/hm23 processing respectively by
14.15,14.21 and 14.34 before medicine drop to 7.21,8.37 and 9.23. respectively reach 60.91% with respect to control effect,
54.83% and 50.60%；Disease index is by 14.27 before medicine after the medicine of 25% triazolone WP 675g/hm2 processing of comparison medicament
It is 61.43% to drop to 7.17. control effects：And the disease index of blank control rises to 18.42% by the l4.13 before medicine.
7d after second of dispenser, microbial liquid fertilizer 75,150, the average disease index difference of 225kg/hm23 processing
It is 8.56,10.46 and 13.22, opposite control effect respectively reaches 68.93%, 62.18% and 52.64%；The disease of comparison medicament
Feelings index and control effect are respectively 8.98 and 67.69%：The disease index of blank control rises to 27.5l%.Variance analysis
The result shows that the control effect between 3 different amounts processing of microbial liquid fertilizer has significant difference：Wherein 225kg/
Hm2 processing and the control effect difference of 25% triazolone WP 675g/hm2 processing of comparison medicament is not notable.
7d after third time dispenser, microbial liquid fertilizer 75,150, the average disease index difference of 225kg/hm23 processing
It is 8.28,11.44 and 15.22, control effect is respectively 77.12%, 68.51% and 58.49%；The disease index of comparison medicament
It is respectively 8.69 and 76.I9 with control effect：The disease index of blank district, which continues to rise, reaches 36.13.Variance analysis knot
Fruit shows that the control effect between 3 different amounts processing of microbial liquid fertilizer has significant difference：Wherein 225kg/hm
The control effect difference of processing and 25% triazolone WP675g ,/hm2 processing is not notable.
4 brief summaries and discussion
According to the test of pesticide effectiveness as a result, microbial liquid fertilizer has preferable control effect to powdery mildew of strawberry.It is falling ill
The control effect that initial stage applies 225kg/hm2 dosages in time reaches as high as 77.12%. to crop safety without poisoning.It is recommended that production
Middle control powdery mildew of strawberry dispenser interval is that 7d. starts medication regarding progression of the disease using 2-3 times in early stage.Withered grass bud
In terms of plant disease control, use should aim at prevention spore bacillus, can not only improve control effect, reduce usage amount,
Cost accounting can also be reduced, crop quality and yield is improved, is especially beneficial and ensures food safety.
Test example 2
The droop field experiment effect of cucumber
Microbial liquid fertilizer made from the above embodiment of the present invention 2,50% carbendazim WP, commercial liquid fertilizer (Henan
High nitrogen and potassium suspension type liquid fertilizer).
1, experimental design：
By field trial, the microbial liquid fertilizer, 50% carbendazim WP and commercial liquid fertilizer are in cucumber growth
Phase to the specifically used effect of droop.
2, materials and methods
2.1 test site
It tests in 2016-2017, there is the towns the Wang Lu villages the Xu Jia cucumber booth for improving drip irrigation system in Jining City Yutai County
It carries out.
2.2 soil overviews
Soil property is based on loam, good permeability.
2.3 for studying object
The close thorn cucumber in low form Xintai City.
2.4 test method
Sample plot is cucumber second crop soil, and the previous year morbidity is heavier, and in November, 2016 field planting suitably controls water and promotes root after field planting
System's development, management routinely carries out in greenhouse after field planting.Greenhouse sets up test block, experimental plot random alignment, cell face respectively
Product is 25m2, is repeated 4 times, and sets protection row between each processing, experiment set microbial liquid fertilizer formulation rate as 75,150,225kg/
Hm23 gradient, 50% carbendazim WP formulation rate of comparison medicament are 675g/hm2, and commercial liquid fertilizer is sprayed in blank control (CK).Medicine
Agent is routinely sprayed.Leave dual sides are uniformly sprayed, continuous dispenser 3 times does not apply other medicaments per during minor tick 15d. experiments
Controlling disease；Fertilizer and microbial liquid fertilizer facility trickle irrigation.
2.5 investigation and calculating
Before dispenser, after first time medicine after 7d, second of medicine after 7d and third time medicine 7d totally 4 investigation strawberries morbidity feelings
Condition uses diagonal line five point sampling, 3 plants of difference record melons quality of every investigation, every plant of fruit number and 10 plants of productions per cell
Amount.Cucumber fusarium axysporum incidence is investigated, according to droop severity Scaling canonical statistics diseased plant number, disease index and preventions at different levels
Effect：
3. result and analysis
Field control effect of the 3.1 microbial liquid fertilizer to cucumber fusarium axysporum
Cucumber respectively do not fall ill by processing, but field incidence trend is that 30d morbidities are lighter after transplanting, and 30-45d progression of the disease is fast
Speed, 45-60d progression of the disease are slowly gradually stablized.Mix bacterium agent is significantly lower than liquid with carbendazim processing droop disease index
Fertilizer control.
60d microbial liquid fertilizer prevents droop effect after the transplanting of 4 cucumber of table
| Processing | Droop diseased plant rate % | Disease index | Control effect % |
| Microbial liquid fertilizer 225kg/hm2 | 1.9d | 1.4c | 81.8a |
| Microbial liquid fertilizer 150kg/hm2 | 2.6c | 1.8c | 76.6b |
| Microbial liquid fertilizer 75kg/hm2 | 3.2b | 2.3b | 70.1c |
| 25% triazolone WP675g/hm2 | 2.1d | 1.5c | 80.5a |
| Commercially available liquid fertilizer 150kg/hm2 | 10.3 | 7.7a |
60d microbial liquids fertilizer can be seen that (table 2) field efficacy of cucumber fusarium axysporum after transplanting, 3 differences
Treating capacity bacterium solution is to cucumber fusarium axysporum preventive effect between 70.1-81.8%.Wherein microbial liquid fertilizer 225kg/hm2 prevention effects
Fruit is best, suitable with the preventive effect of 50% carbendazim WP.
Safety of the 3.2 microbial liquid fertilizer to cucumber growth
Cucumber at seedling stage plant height, the investigation result of 3 indexs of the number of blade and radical show (table 5), although different disposal amount
Cucumber plant growing state shows certain difference, but microbial liquid fertilizer 225kg/hm2 processing and 50% carbendazim WP
It is normal that performance growth is compared with commercial liquid soil fertility quality, illustrates microbial liquid fertilizer to cucumber growth safety, without bad
It influences.
Safety of the 5 microbial liquid fertilizer of table to cucumber growth
Influence of the 3.3 microbial liquid fertilizer to cucumber yield
Cell field trial shows that microbial liquid fertilizer has cucumber yield certain production-increasing function, different disposal amount
Rate of growth there are significant differences, wherein yield highest after the microbial liquid soil fertility quality of 225kg/hm2 dosages, than commercially available liquid
The volume increase 10.0% of body fertilizer is also notable with 50% carbendazim processing difference.
4 brief summaries and discussion
According to the test of pesticide effectiveness as a result, microbial liquid fertilizer has preferable control effect to cucumber fusarium axysporum, to cucumber
Growth has no adverse effects, and can be used for the prevention of cucumber fusarium axysporum.The wherein microbial liquid fertilizer prevention of 225kg/hm2 dosages
Effect and production-increasing function behave oneself best and stablize, control effect and 50% carbendazim WP being commonly used in traditional agriculture production
Compared to no significant difference, safety of human and livestock acts on environment nonhazardous as a kind of biological prevention and control agent, safety is obviously excellent
In chemicals, microbial liquid fertilizer also has certain production-increasing function in addition, can also reduce cost accounting, improves crop
Quality and yield is especially beneficial and ensures food safety.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of complex microorganism liquid fertilizer, which is characterized in that including microbial inoculum, organic-biological nitrogen, inorganic fertilizer,
Middle amount inorganic fertilizer and bioactivity organic matter.
2. complex microorganism liquid fertilizer according to claim 1, which is characterized in that include according to mass parts meter：Micro- life
6~15 parts of object bacterium solution, 5~15 parts of organic-biological nitrogen, 10~30 parts of inorganic fertilizer, 3~5 parts of middle amount inorganic fertilizer and biology are lived
15~40 parts of organic matter of property.
3. complex microorganism liquid fertilizer according to claim 1, which is characterized in that include according to mass parts meter：Micro- life
8~14 parts of object bacterium solution, 7~13 parts of organic-biological nitrogen, 15~23 parts of inorganic fertilizer, 3.5~4.5 parts of middle amount inorganic fertilizer and life
18~30 parts of object active organic matter.
4. complex microorganism liquid fertilizer according to claim 2 or 3, which is characterized in that include according to mass parts meter：It is micro-
12 parts of biological bacterium solution, 11 parts of organic-biological nitrogen, 20 parts of inorganic fertilizer, 4 parts of middle amount inorganic fertilizer and bioactivity organic matter 20
Part.
5. complex microorganism liquid fertilizer according to claim 4, which is characterized in that the organic-biological nitrogen is selected from ammonia
Appoint one or more in base acid, polypeptide, protein, Fish protein, liquid molasses.
6. complex microorganism liquid fertilizer according to claim 4, which is characterized in that the inorganic fertilizer is selected from sulfuric acid
Appoint one or more in ammonium, ammonium chloride, ammonium nitrate, sodium nitrate, calcium nitrate, urea, potassium chloride, potassium sulfate and potassium nitrate.
7. complex microorganism liquid fertilizer according to claim 4, which is characterized in that the middle amount inorganic fertilizer is selected from chlorine
Appointing in change calcium, calcium nitrate, amino acid chelated calcium, ethylenediamine tetra-acetic acid chelating calcium, magnesium chloride, magnesium sulfate and magnesium nitrate is one or more
Kind.
8. complex microorganism liquid fertilizer according to claim 4, which is characterized in that bioactivity organic matter is selected from amino
Appoint one or more in acid, polypeptide, protein, Fish protein, liquid molasses.
9. complex microorganism liquid fertilizer according to claim 8, which is characterized in that the preparation side of the microbial inoculum
Method includes step：
Culture medium is separately added into the first tank and the second tank, is closed, is sterilized；
After the completion of sterilizing, respectively cool down to it processing, ensures that tank pressure is 0.07~0.14Mpa in temperature-fall period；
When temperature drops to 28~36 DEG C, the first tank of constant temperature and pressure pair is inoculated with；Ferment 9~12h, and the zymotic fluid of the first tank is added
Enter to the second tank and relay supervention ferment, control condition puts tank to get microbial bacteria with the first tank when thalline forms 90~95%
Liquid.
10. complex microorganism liquid fertilizer according to claim 9, which is characterized in that the bacterium of inoculation is selected from withered grass bud
Appoint one or more in spore bacillus, bacillus licheniformis, bacillus megaterium, glue fat bacillus and Bacillus cercus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810184377.5A CN108395319A (en) | 2018-03-06 | 2018-03-06 | A kind of complex microorganism liquid fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810184377.5A CN108395319A (en) | 2018-03-06 | 2018-03-06 | A kind of complex microorganism liquid fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108395319A true CN108395319A (en) | 2018-08-14 |
Family
ID=63092018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810184377.5A Pending CN108395319A (en) | 2018-03-06 | 2018-03-06 | A kind of complex microorganism liquid fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108395319A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109053298A (en) * | 2018-10-18 | 2018-12-21 | 舟山丰宇三立海洋生物肥料有限公司 | A kind of dedicated fertilizer and preparation method thereof improving fruits and vegetables cold tolerance |
| CN109608255A (en) * | 2018-12-25 | 2019-04-12 | 安徽伽雅生态工程有限公司 | A kind of preparation method of liquid bio-organic fertilizer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1422828A (en) * | 2002-12-20 | 2003-06-11 | 黎保文 | Microbial compound liquid fertilizer and method for preparing the same |
| WO2010104197A1 (en) * | 2009-03-13 | 2010-09-16 | アサヒビール株式会社 | A reducing mixture derived from microorganisms which has an oxidation-reduction potential of 0mv or less, and production method for same |
| CN101844944B (en) * | 2009-03-23 | 2013-04-24 | 北京世纪阿姆斯生物技术股份有限公司 | Total nutrient liquid microbial fertilizer |
-
2018
- 2018-03-06 CN CN201810184377.5A patent/CN108395319A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1422828A (en) * | 2002-12-20 | 2003-06-11 | 黎保文 | Microbial compound liquid fertilizer and method for preparing the same |
| WO2010104197A1 (en) * | 2009-03-13 | 2010-09-16 | アサヒビール株式会社 | A reducing mixture derived from microorganisms which has an oxidation-reduction potential of 0mv or less, and production method for same |
| CN101844944B (en) * | 2009-03-23 | 2013-04-24 | 北京世纪阿姆斯生物技术股份有限公司 | Total nutrient liquid microbial fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109053298A (en) * | 2018-10-18 | 2018-12-21 | 舟山丰宇三立海洋生物肥料有限公司 | A kind of dedicated fertilizer and preparation method thereof improving fruits and vegetables cold tolerance |
| CN109608255A (en) * | 2018-12-25 | 2019-04-12 | 安徽伽雅生态工程有限公司 | A kind of preparation method of liquid bio-organic fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN102329754B (en) | Novel strain of streptomyces fradiae and application thereof | |
| CN103342604B (en) | Bio-control bacterium foliage fertilizer, and preparation method and application thereof | |
| CN101289338B (en) | Biological salt-decomposition bacterial manure and method for preparing same | |
| CN106495826A (en) | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer | |
| CN109971680A (en) | A kind of preparation and application of the complex micro organism fungicide with disease-resistant growth-promoting function | |
| CN104609997A (en) | Manufacturing process and application method of ginseng bio-organic fertilizer | |
| CN106190137B (en) | A kind of saline-alkali land soil conditioner and its preparation method and application | |
| CN102816726A (en) | Novel compound microbial agent and applications thereof | |
| CN106591193A (en) | Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects | |
| CN109896900A (en) | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer | |
| CN107759420A (en) | A kind of Composite microbial fertilizer for paddy | |
| CN105175178A (en) | Anti-continuous-cropping microbial fertilizer and preparation method therefor | |
| CN107556093A (en) | A kind of special hybrid bacterial strain microbial manure of solanaceous vegetables and preparation method thereof | |
| CN107409928A (en) | A kind of implantation methods in organic orchard | |
| CN103772011A (en) | Special compound biological liquid-state bacterial fertilizer for grain | |
| CN106242829A (en) | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage | |
| CN108029501A (en) | A kind of vegetable nutrient diseases prevention seedling medium and preparation method and application | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN108640759A (en) | A kind of preparation method of organic fertilizer for the sweetened expelling parasite of melon and fruit | |
| CN101298403A (en) | Bioorganic agriculture fertilizer and preparation thereof | |
| CN108863531A (en) | A kind of ferment fertilizer and preparation method thereof with facilitating effects of taking root | |
| CN105255762A (en) | Micro-ecology preparation for soil conditioning | |
| CN108395319A (en) | A kind of complex microorganism liquid fertilizer | |
| CN109836298A (en) | A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180814 |
|
| RJ01 | Rejection of invention patent application after publication |