CN108379321A - A kind of golden flower barberries extract and its preparation method and application - Google Patents
A kind of golden flower barberries extract and its preparation method and application Download PDFInfo
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- CN108379321A CN108379321A CN201810437267.5A CN201810437267A CN108379321A CN 108379321 A CN108379321 A CN 108379321A CN 201810437267 A CN201810437267 A CN 201810437267A CN 108379321 A CN108379321 A CN 108379321A
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- extract
- barberries
- golden flower
- stem
- root
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The present invention provides a kind of golden flower barberries extracts and its preparation method and application.Present invention firstly provides a kind of golden flower barberries extracts, it is prepared by following methods：(1) root, stem, leaf of golden flower barberries are taken respectively, are crushed, are obtained raw material powder；(2) raw material powder is taken, is extracted respectively to get root, the ethanol extract of stem, leaf with ethyl alcohol.The results show, golden flower barberries ethanol extract and n-butanol extract make moderate progress to the memory capability of mouse, apparent to the improvement result of dysmnesia model mouse.
Description
Technical field
The present invention relates to a kind of golden flower barberries extracts and its preparation method and application.
Background technology
Berberis (Berberis L.) is a category in Berberidaceae (Berberidaceae), practises and is known as " barberry ",
Also referred to as copper needle thorn, soulie barberry bark, thorn Cortex Phellodendri etc., type is various, and the whole world is distributed in Asia, South and North America, Europe there are about 500 kinds
Continent and Africa are northern.In Chinese berberis there are about more than 250 kinds, it is mainly distributed on the western and west and south.
China has a long history to the utilization of berberis, a long time ago, civil to commonly use its rhizome to replace Huang
Cypress, coptis medicinal, take root to decoct, and are used as clearing heat and detoxicating Chinese medicine common simply, therapeutic domain is wide, curative for effect.It is small
Bark of a cork tree platymiscium it is medicinal first recorded in《Tang materia medica》, Tao Hongjing is referred to as sub- bark of a cork tree, is stated as：" the sub- small shape of bark of a cork tree tree such as pomegranate, Beijing opera and
Hardship, another thorniness, Pi Yihuang, also to aphtha ".Chen Cangqi《Bencao Shiyi》Description is specifically：" barberry such as pomegranate, skin
Huang Zichi, such as fruit of Chinese wolfberry, pointed at both ends small, file branch is to contaminate Huang ".《Compendium of Materia Medica》Record：" there is it when barberry intermountain, little tree,
It is white outside its skin, inner Huang, shape such as bark of a cork tree skin and it is thin small ".Pharmacological research thinks that the extract of barberry has antiphlogistic antibacterial, blood pressure lowering, drop
The pharmacological activity such as blood fat, antitumor, hypoglycemic, Recent study find that its anti-oxidation function is also fairly good.
Golden flower barberries (Berberis wilsonae Hemsl) are the plant of Berberidaceae Berberis, are half evergreen dwarf shrub.
The relevant report of golden flower barberries extract of the present invention and its preparation method and application is had no at present.
Invention content
The purpose of the present invention is to provide a kind of golden flower barberries extracts and its preparation method and application.
Present invention firstly provides a kind of golden flower barberries extracts, it is prepared by following methods：
(1) root, stem, leaf of golden flower barberries are taken respectively, are crushed, are obtained raw material powder；
(2) raw material powder is taken, is extracted respectively to get root, the ethanol extract of stem, leaf with ethyl alcohol.
Further, in step (2), a concentration of 65%~95%V/V of the ethyl alcohol, it is preferable that the ethyl alcohol it is dense
Degree is 95%V/V.
Further, preparation method is further comprising the steps of：The ethanol extract for taking root, stem, with extracting n-butyl alcohol, i.e.,
Obtain the n-butanol extract of root, stem.
The present invention also provides the preparation methods of above-mentioned golden flower barberries extract, it includes the following steps：
(1) root, stem, leaf of golden flower barberries are taken respectively, are crushed, are obtained raw material powder；
(2) raw material powder is taken, is extracted respectively to get root, the ethanol extract of stem, leaf with ethyl alcohol.
Further, in step (2), a concentration of 65%~95%V/V of the ethyl alcohol, it is preferable that the ethyl alcohol it is dense
Degree is 95%V/V.
The present invention also provides the preparation methods of above-mentioned golden flower barberries extract, it includes the following steps：Take the above method
The ethanol extract of the root, stem that are prepared, with extracting n-butyl alcohol to get root, the n-butanol extract of stem.
The present invention also provides above-mentioned golden flower barberries extracts to prepare the purposes in preventing medicine for senile dementia.
Further, the senile dementia is Alzheimer disease, vascular dementia, Mixed dementia.
The present invention also provides it is a kind of prevention senile dementia drug, it be with above-mentioned golden flower barberries extract be activity at
Point, in addition the preparation that pharmaceutically acceptable auxiliary material is prepared.
Further, the preparation is powder, granule, tablet, capsule.
The results show, golden flower barberries ethanol extract and n-butanol extract change the memory capability of mouse
It is kind, it is apparent to the improvement result of dysmnesia model mouse.
Obviously, the above according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific implementation mode of form by the following examples remakes further specifically the above of the present invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on the above of the present invention
The technology realized all belongs to the scope of the present invention.
Description of the drawings
Fig. 1 is influence of the golden flower barberries extract to mouse weight.
Fig. 2 is influence of the golden flower barberries extract to mouse step down test.
Fig. 3 is influence of the golden flower barberries extract to mouse orientation navigation.
Fig. 4 is influence of the golden flower barberries extract to mouse space exploration.
Specific implementation mode
The preparation of embodiment 1, golden flower barberry ethanol extracts
Each 300g of root, stem, leaf for choosing golden flower barberries after drying and crushing, uses the 95%V/V ethyl alcohol of 3000mL to extract respectively
3 times, 40min is extracted every time, merges extracting solution, up to root, stem, leaf extract after concentrate drying.
The preparation of embodiment 2, golden flower barberry n-butanol extracts
Each 300g of root, stem for choosing golden flower barberries respectively after drying and crushing, is respectively carried for 3 times with 95% ethyl alcohol of 3000mL point
It takes, extracts 40min every time；It is concentrated respectively after merging extracting solution, obtains the medicinal extract 24.22g of root, the medicinal extract 12.39g of stem；Distinguish again
0.1%~1% aqueous hydrochloric acid solution dissolving of 200mL is added, filtering takes filtrate；Filtrate respectively adds the 0.5%NaOH solution of 100mL,
Shake 10min；Finally divide 2 extractions with 150~200mL n-butanols respectively, is concentrated after combining extraction liquid, respectively obtain root, stem
N-butanol segment.
The preparation of embodiment 3, golden flower barberry extracts
Each 300g of root, stem, leaf for choosing golden flower barberries after drying and crushing, uses the 65%V/V ethyl alcohol of 3000mL to extract respectively
3 times, 40min is extracted every time, merges extracting solution, up to root, stem, leaf extract after concentrate drying.
The preparation of embodiment 4, golden flower barberry extracts
Each 300g of root, stem, leaf for choosing golden flower barberries after drying and crushing, uses the 80%V/V ethyl alcohol of 3000mL to extract respectively
3 times, 40min is extracted every time, merges extracting solution, up to root, stem, leaf extract after concentrate drying.
Illustrate beneficial effects of the present invention below by way of test example.
Test example 1, golden flower barberry extracts improve the research of mouse memory power
(1) experimental method
It selects the male mouse of kunming mouse that weight is 20 ± 2g to carry out Jumping test, water maze laboratory, and measures mouse
MDA contents in serum and T-SOD vigor.Mouse is raised by weight average grouping.According to gavage condition, will remember again
Existing deficiency model mouse is divided into 5 groups：The root of golden flower barberries, each one group of the high, medium and low dosage of stem, leaf extract, stem n-butanol
Segment group, root n-butanol segment group, it is another to set normal mouse blank group and each 1 group of model group, every group of 8 mouse.Extract
High, medium and low dose concentration be set to 100mg/mL, 50mg/mL, 25mg/mL, root, stem n-butanol segment concentration (w/
Ml it is) 2.1%, 1.64%, continuous processing 27 days.
Ensure that animal in 23 ± 2 DEG C of temperature, free water and feed, controls illumination/dark 12h and follow daily during raising
Ring, 8 points of stopping illumination of night, starts gloss at 8 points between morning.Before experiment starts, Animal adaptability feeds 3d.When first day gavage,
It is weighed, is weighed every three days later primary, nominal weighs 10 times for the first time.
Jumping test：Experimental provision is a rectangle reflective box, is divided between 2, and it is 0.5cm copper grid that bottom surface, which is aided with spacing, often
Between left or right angle place the insulating stand that a height and diameter are 4.5cm.It is formal to measure first 1 day of experiment, after intragastric administration on mice
Mouse is put into electroporation chamber by 30min, and shocked by electricity trained 5min with 100uA electric currents, and mouse is made to generate electric shock memory.It rejects to electric shock
Insensitive mouse is stimulated, carries out formal determination experiment after r for 24 hours, after mouse last time gavage 10min, then gavage is a certain amount of
30% ethyl alcohol (according to mouse weight, ethyl alcohol gavage volume converts by 0.1ml/g), form memory and reproduce deficiency model mouse.
Animal is placed on diving tower after 30min, record mouse jumps off every in the time (incubation period) and 5min of diving tower for the first time
Mouse is total (errors number) by the number to shock by electricity.
Water maze laboratory：Experimental provision is an a diameter of 130cm, the round pool of deep 40cm, artificially by pond point
It for four quadrants and marks, platform fixed placement is central in one of quadrant, and in pond plus water is added to beyond platform 1-
2cm high, water temperature are controlled at 23 ± 2 DEG C.Formal test a few days ago by mouse head along pool wall into the water, make every by permanent order
Mouse enters water from 4 quadrants, measure animal swim in labyrinth total distance, where platform quadrant (target quadrant) time,
The number of swimming average speed and spanning platform, the time average that animal finds platform are incubation period, and each quadrant is dived
Volt phase average value is incubation period on the same day, using the 4th day data as the final result.
Animal is a few days ago put into progress adaptability swimming in water maze by formal test, rejects the individual that do not swim, formally
Experiment 5 days in total, first 4 days are orientation navigation experiment.1h is in addition to blank group injecting normal saline after first day gavage, other each groups
Animal causes acquired dysmnesia model with 0.1ml/10g weight conversion volume intraperitoneal injection hyoscine (3mg/kg).
Mouse is put into free swimming in water maze after 20min, platform the time it takes is found in 60S and is denoted as incubation period, in platform
Stop 5S after, animal is taken out, if animal does not find platform in 60S, by artificially guide animal swim to platform and allow it
Platform stops 5S, is denoted as incubation period 60S；Record mouse swimming total distance, incubation period, target quadrant time percentage after experiment four days
Than and average speed；5th day for space exploration test, space exploration test when platform is removed, allow animal from original platform as
The quadrant on limit opposite enters water, and record animal enters the number of original platform quadrant time and spanning platform.
(2) statistical analysis
Variance analysis is carried out with SPSS18.0 statistical softwares, it is aobvious to indicate that difference has with P ＜ 0.01 for comparison among groups LSD methods
Work property, result data is indicated with means ± std.
(3) experimental result
It will be seen from figure 1 that experimental group with normally group mouse compared with, after gavage 1d, 10d, 19d, 30d,
Each group the weight of animals is not significantly different (P ＞ 0.05), illustrates that golden flower barberries extract influences mouse weight without obvious.
Figure it is seen that experimental group is compared with model group, incubation period obviously increases, and errors number significantly reduces, to surveying
Data it is for statistical analysis obtain experimental group and model group difference extremely significantly (P ＜ 0.01), not with normal group contrast difference
Significantly (P ＞ 0.05).
From figure 3, it can be seen that compared with normally group total distance, model group animal does not have difference；With the target normally organized as
Limit percentage of time equal indifference in addition to model group；Compared with normally group incubation period, model group there were significant differences (P<0.05), root
Extract middle dose group, leaf extract are high, middle dose group has notable or pole significant difference, other group of indifference；It is flat with normal group
Equal speed compares, model group indifference.Compared with model group total distance, the equal no significant difference of each group；With model group target quadrant
Percentage of time compares, and in addition to stem extraction low dose group, other groups have notable or pole significant difference；With model group incubation period
Compare, all dosage groups of root extract low dose group, leaf extract, root n-butanol segment group and stem n-butanol segment group indifference
Different, other groups have notable or pole significant difference；Compared with model group average speed, indifference is normally organized.
From fig. 4, it can be seen that compared with model group animal, in addition to the height of stem extraction, middle dose group and stem n-butanol piece
Section group is outer, and remaining Group Animals spanning platform number dramatically increases (P≤0.05)；With model group animal when target quadrant stops
Between compare, the difference of all groups of target quadrant time percentages is extremely significantly (P<0.01)；Compared with normally group animal, model
Group spanning platform number is reduced, and there were significant differences (P≤0.05), and target quadrant time percentage is reduced, and has pole significant difference (P<
0.01), experimental group and normal group compare, except stem extraction high dose group traversing times have significant decrease (P≤0.05), other groups
Compared with normal group, the equal indifference of two indices (P ＞ 0.05).
To sum up, golden flower barberries ethanol extract and n-butanol extract make moderate progress to the memory capability of mouse, to note
The improvement result for recalling Disorder Model mouse is apparent.
Claims (10)
1. a kind of golden flower barberries extract, it is characterised in that：It is prepared by following methods：
(1) root, stem, leaf of golden flower barberries are taken respectively, are crushed, are obtained raw material powder；
(2) raw material powder is taken, is extracted respectively to get root, the ethanol extract of stem, leaf with ethyl alcohol.
2. golden flower barberries extract according to claim 1, it is characterised in that：In step (2), the ethyl alcohol it is a concentration of
65%~95%V/V, it is preferable that a concentration of 95%V/V of the ethyl alcohol.
3. extract according to claim 1 or 2, it is characterised in that：Preparation method is further comprising the steps of：Take root,
The ethanol extract of stem, with extracting n-butyl alcohol to get root, the n-butanol extract of stem.
4. the preparation method of golden flower barberries extract described in claims 1 or 2, it is characterised in that：It includes the following steps：
(1) root, stem, leaf of golden flower barberries are taken respectively, are crushed, are obtained raw material powder；
(2) raw material powder is taken, is extracted respectively to get root, the ethanol extract of stem, leaf with ethyl alcohol.
5. preparation method according to claim 4, it is characterised in that：In step (2), the ethyl alcohol a concentration of 65%~
95%V/V, it is preferable that a concentration of 95%V/V of the ethyl alcohol.
6. the preparation method of golden flower barberries extract described in claim 3, it is characterised in that：It includes the following steps：Weighting profit
It is required that 5 or 6 be prepared root, stem ethanol extract, with extracting n-butyl alcohol to get root, the n-butanol extract of stem.
7. claims 1 to 3 any one of them golden flower barberries extract is preparing the purposes in preventing medicine for senile dementia.
8. purposes according to claim 7, it is characterised in that：The senile dementia is Alzheimer disease, vascular
Dull-witted, Mixed dementia.
9. a kind of drug of prevention senile dementia, it is characterised in that：It is with any one of claims 1 to 3 golden flower barberries
Extract is active constituent, in addition the preparation that pharmaceutically acceptable auxiliary material is prepared.
10. drug according to claim 9, it is characterised in that：The preparation is powder, granule, tablet, capsule.
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| CN201810437267.5A CN108379321A (en) | 2018-05-09 | 2018-05-09 | A kind of golden flower barberries extract and its preparation method and application |
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| CN201810437267.5A CN108379321A (en) | 2018-05-09 | 2018-05-09 | A kind of golden flower barberries extract and its preparation method and application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843618A (en) * | 2010-02-26 | 2010-09-29 | 复旦大学 | Application of berberine and derivatives thereof in preparation of indole amine 2, 3-dioxygenase inhibitor |
| CN103989678A (en) * | 2014-04-24 | 2014-08-20 | 香港理工大学 | Composition for preventing and treating Alzheimer's disease, and its application |
-
2018
- 2018-05-09 CN CN201810437267.5A patent/CN108379321A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843618A (en) * | 2010-02-26 | 2010-09-29 | 复旦大学 | Application of berberine and derivatives thereof in preparation of indole amine 2, 3-dioxygenase inhibitor |
| CN103989678A (en) * | 2014-04-24 | 2014-08-20 | 香港理工大学 | Composition for preventing and treating Alzheimer's disease, and its application |
Non-Patent Citations (2)
| Title |
|---|
| 冯章英等: "常用中药提取物对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制活性评价", 《中国药理学与毒理学杂志》 * |
| 王柳卜等: "三颗针中盐酸小檗碱、盐酸药根碱及盐酸巴马汀的含量测定", 《时珍国医国药》 * |
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Application publication date: 20180810 |