CN105708899B - Traditional Chinese medicine composition for treating acute alcoholic liver injury and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating acute alcoholic liver injury and preparation method thereof Download PDFInfo
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- A61K36/06—Fungi, e.g. yeasts
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Abstract
The invention discloses a traditional Chinese medicine composition for treating acute alcoholic liver injury, which is prepared from dracocephalum heterophyllum, thalictrum herb, salvia praecox, gentiana straminea and yellow mushroom. The invention also discloses a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition provided by the invention has a synergistic effect after 5 traditional Chinese medicines such as dracocephalum heterophyllum are combined for use, has an obvious effect of treating acute alcoholic liver injury, can obviously improve the pathological manifestations of liver injury, particularly acute alcoholic liver injury, and provides a new choice for clinical medication.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with an effect of treating acute alcoholic liver injury and a preparation method thereof.
Background
In recent years, with the improvement of the living standard and the change of the dietary structure of China, the consumption of alcohol is increasing day by day, and the incidence rate of alcoholic liver injury tends to rise year by year in China. Alcoholic liver injury has now become the second largest liver disease after viral hepatitis. Alcoholic liver injury is a progressive disease, can cause liver dysfunction such as hepatocyte necrosis, hepatic steatosis, liver cirrhosis and the like to different degrees, can seriously harm the health of a body in the later period, and can finally cause liver cancer. Alcoholic liver injury is characterized by general susceptibility and short incubation period in people, and the pathological change degree of the alcoholic liver injury is gradually aggravated along with the increase of the infection degree.
Alcoholic injury is a common liver disease in developed countries, and no specific medicine is available up to now. For people who drink alcohol, the fundamental measure for improving the body state is to abstain alcohol, but alcohol addiction makes it difficult for most drinkers to completely abstain alcohol, so that when people who drink alcohol in a proper amount drink alcohol, a traditional Chinese medicine composition with a liver protection function is urgently needed to prevent the damage of alcohol to the liver. At present, dozens of traditional Chinese medicines with liver injury resistance are reported, such as rhubarb, cordyceps, astragalus, salvia, medlar, schisandra and the like, wherein medicinal and edible Tibetan medicinal materials are not lacked. Therefore, the medicine for preventing liver injury is searched from the medicinal materials, and has important significance.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for treating acute alcoholic liver injury.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition for treating acute alcoholic liver injury.
A traditional Chinese medicine composition for treating acute alcoholic liver injury is characterized by being prepared from dracocephalum heterophyllum benth, thalictrum hypoglaucum, salvia praecox, gentiana straminea and yellow mushroom.
Further, a traditional Chinese medicine composition with the effect of treating acute alcoholic liver injury, which is characterized by comprising the following components in parts by weight: the preparation is prepared from the following raw material medicines in parts by weight:
12-16 parts of dracocephalum heterophyllum benth, 5-8 parts of saxifraga tangutica, 3-5 parts of salvia pratense, 8-10 parts of gentiana straminea and 10-12 parts of yellow mushroom.
Further, the traditional Chinese medicine composition is characterized in that: the preparation is prepared from the following raw material medicines in parts by weight:
12 parts of dracocephalum heterophyllum benth, 5 parts of Thangostereum glaucocalyx, 5 parts of Kangding sage, 10 parts of gentiana straminea and 10 parts of yellow mushroom.
Further, the traditional Chinese medicine composition is characterized in that: the preparation is prepared from the following raw material medicines in parts by weight: or 16 parts of dracocephalum heterophyllum benth, 8 parts of Thomson saxifraga herb, 3 parts of Kangding sage herb, 8 parts of Gentiana straminea Maxim and 12 parts of yellow mushroom.
Further, the traditional Chinese medicine composition is characterized in that: the preparation is prepared by taking medicinal powder which is prepared by crushing and uniformly mixing raw material medicines according to a conventional method or water or/and ethanol extract of the raw material medicines as active ingredients and adding pharmaceutically common auxiliary materials or auxiliary ingredients.
Further, the traditional Chinese medicine composition is characterized in that: the preparation is an oral preparation.
Further, the traditional Chinese medicine composition is characterized in that: the oral preparation is tablet, pill, capsule, powder or oral liquid.
The preparation method of the traditional Chinese medicine composition is characterized by comprising the following steps:
(1) weighing the raw materials according to the proportion;
(2) pulverizing the raw materials weighed in the step (1) and uniformly mixing to obtain medicinal powder, or extracting with water or/and ethanol according to a conventional method to obtain an extract;
(3) and (3) adding common auxiliary materials or auxiliary components into the medicinal powder or the extract obtained in the step (2) to prepare a preparation.
Further, the application of the traditional Chinese medicine composition in preparing food, health-care products or medicines for treating and preventing acute alcoholic liver injury.
Compared with the prior art, the invention has the beneficial effects that:
the traditional Chinese medicine composition provided by the invention has the advantages that after the 5 medicines such as dracocephalum heterophyllum benth and the like are combined for use, the synergistic effect is exerted, the traditional Chinese medicine composition has an obvious effect of treating acute alcoholic liver injury, can obviously improve the pathological manifestations of liver injury, particularly acute alcoholic liver injury, and provides a new choice for clinical medication.
Detailed Description
Example 1
Decocting herba Dracocephali 12g, herba Saxifragae 5g, herba Salvia officinalis 5g, radix Gentianae Marcrophyllae 10g, and yellow mushroom 10g in water for 20 min for 3 times, and mixing water decoctions to obtain decoction.
Example 2
16g of dracocephalum heterophyllum benth, 8g of saxifraga tangutica, 3g of Kangding sage herb, 8g of gentiana straminea maxim and 12g of yellow mushroom, and carrying out reflux extraction on the raw materials for 3 times with 60-80% v/v ethanol, wherein each time lasts for 30 minutes, and the ethanol extract is reserved; decocting the residue in water for 2 times, each for 20 min, mixing the water decoctions, recovering ethanol from the ethanol extractive solution, mixing with the water decoction, concentrating, adding appropriate amount of adjuvants, and making into granule.
Example 3
15g of dracocephalum heterophyllum benth, 6g of Thangste saxifrage, 4g of Kangding sage herb, 9g of gentiana straminea Maxim and 11g of yellow mushroom, adding water, soaking for 3 times at 80 ℃ for 2 hours each time, combining the extracting solutions, concentrating, adding a proper amount of ethanol, completely precipitating, taking the precipitate, drying for later use, concentrating the supernatant, adding a proper amount of microcrystalline cellulose, spray-drying, uniformly mixing the obtained dried product with the precipitate, and encapsulating to obtain the capsule.
The advantageous effects of the present invention will be specifically described below by way of test examples
1. Experimental Material
1.1 the experimental medicines are set into different dosage proportioning groups, namely proportioning group 1 (Isolobayenne greenbelt: Thangtish saxifrage: Kangding sage: Gentiana macrophylla: 2.4: 1: 1: 2: 2, namely 12g of Isolobayenne greenbelt, 5g of Thangtish saxifrage, 5g of Kangding sage, 10g of Gentiana straminea, 10g of yellow mushroom); proportioning group 2 (Isolobata (Clerodendron glaucophyllum: Thanggu saxifrage: Salvia pratense: Gentiana macrophylla: Flammulina davidii ═ 2: 1: 0.375: 1: 3, namely Isolobata (Clerodendrum glaucophyllum) 16g, Thanggu saxifrage (Thalictrifolium) 8g, Salvia pratense 3g, Salvia praecox 8g, and Hypsizygus marmoreus 12 g); proportioning group 3 (Isolobata (Dracocephalum heterophyllum (Turkey Saxifraga) and Salvia praecox (Maxim.) Maxim. 2: 0.8: 0.53: 1.2: 1.47, namely Isolobata (Turkey Holly) 15g, Salvia praecox (Turkey Saxifraga) 6g, Salvia praecox 4g, Salvia praecox 9g, and Hypsizygus marmoreus 11 g); decocting the above three groups in water for 3 times, each for 20 min, and concentrating the obtained extractive solutions to 60g/100mL respectively.
1.2 Experimental animals: kunming mice (license number: SCXK (Shanghai Si Laike) 2012-0036 provided by Shanghai Si Laike laboratory animals Co., Ltd.), weight of 18-22 g, half male and half female.
1.3 Experimental reagents: serum glutamic-pyruvic transaminase (ALT) determination kit (batch number: 20140820), serum glutamic-oxaloacetic transaminase (AST) determination kit (batch number: 20140909), serum Triglyceride (TG) determination kit (batch number: 20140825) and liver Malondialdehyde (MDA) determination kit (batch number: 20140902), all of which are purchased from Nanjing institute of bioengineering.
1.4 Instrument: ultraviolet-visible spectrophotometer, refrigerated centrifuge.
2. Experimental methods
2.1 Effect on serum ALT of mice with acute alcoholic liver injury
60 mice were randomly divided into 5 groups, blank, model, ratio 1, ratio 2 and ratio 3. Except for the blank group, each group was intragastrically injected with 55% ethanol at a weight of 15mL/Kg, after 1 hour, each group was intragastrically injected with a medicinal solution at a weight of 0.2mL/10g, and the blank group and the model group were administered with the same volume of physiological saline for 14 days continuously. Mice were sacrificed by eye-picking without food deprivation for 16h after the last dose. Blood is taken for separating serum, ALT is measured according to a kit method, and the result of each administration group is compared with the result of a model control group for variance analysis.
2.2 Effect on serum AST of mice with acute alcoholic liver injury
The experimental groups and the dose were the same as those in item 2.1. And (4) taking blood, separating serum, detecting AST according to a kit method, and comparing the result of each administration group with the result of a model control group for analysis of variance.
2.3 Effect on serum TG in mice with acute alcoholic liver injury
The experimental groups and the dose were the same as those in item 2.1. Blood is taken for separating serum, TG is measured according to a kit method, and the result of each administration group is compared with the result of a model control group for variance analysis.
2.4 Effect on liver tissue MDA of mice with acute alcoholic liver injury
The experimental groups and the dose were the same as those in item 2.1. After the mice are killed, liver tissues are taken for homogenate, MDA is measured according to a kit method, and the result of each administration group is compared with the result of a model control group for variance analysis.
3. The results are shown in Table 1
TABLE 1 Effect of different dose combinations on ALT, AST, TG and MDA in mice (n + -s, n-12)
Note: compared with the model group, a P is less than 0.05, A P is less than 0.01; compared with the proportioning group 3, b P is less than 0.05, B P is less than 0.01
As can be seen from Table 1, compared with the model group, the groups with different proportioning doses of the medicine/health food of the invention can obviously reduce the level of ALT, AST, TG and MDA of the serum of mice. Compared with the compatibility group 2, the effect of the proportioning groups 1 and 3 on ALT, AST and MDA is more obvious.
In conclusion, each matching group can obviously improve each index of a mouse with liver injury, has better effect of treating acute alcoholic liver injury, the treatment effect of the matching groups 1 and 3 is better than that of the matching group 2, and the matching group 3 (Isopteris glauca: Thangstegia gabonensis: Salvia pratense: Gentiana macrophylla: Flammulina velutipes ═ 2: 0.8: 0.53: 1.2: 1.47, namely 15g of Isopteris glauca, 6g of Thangstegia glauca, 4g of Kandiphyllum pratense, 9g of Gentiana straminea, and 11g of yellow mushroom) is the optimal dosage matching ratio by taking the comprehensive factors such as cost and the like into consideration.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A traditional Chinese medicine composition for treating acute alcoholic liver injury is characterized in that: the preparation is prepared from the following raw material medicines in parts by weight: 12-16 parts of dracocephalum heterophyllum benth, 5-8 parts of saxifraga tangutica, 3-5 parts of salvia pratense, 8-10 parts of gentiana straminea and 10-12 parts of yellow mushroom.
2. The traditional Chinese medicine composition according to claim 1, characterized in that: the preparation is prepared from the following raw material medicines in parts by weight: 12 parts of dracocephalum heterophyllum benth, 5 parts of Thangostereum glaucocalyx, 5 parts of Kangding sage, 10 parts of gentiana straminea and 10 parts of yellow mushroom.
3. The traditional Chinese medicine composition according to claim 1, characterized in that: the preparation is prepared from the following raw material medicines in parts by weight: or 16 parts of dracocephalum heterophyllum benth, 8 parts of Thomson saxifraga herb, 3 parts of Kangding sage herb, 8 parts of Gentiana straminea Maxim and 12 parts of yellow mushroom.
4. The traditional Chinese medicine composition according to any one of claims 1 to 3, wherein: the traditional Chinese medicine preparation is prepared by taking medicinal powder obtained by crushing and uniformly mixing raw material medicines according to a conventional method or water or/and ethanol extract of the raw material medicines as active ingredients and adding common auxiliary materials or auxiliary ingredients in traditional Chinese medicine science.
5. The traditional Chinese medicine composition according to claim 4, wherein: the preparation is an oral preparation.
6. The traditional Chinese medicine composition according to claim 5, wherein: the oral preparation is tablet, pill, capsule, powder or oral liquid.
7. The preparation method of the traditional Chinese medicine composition according to claim 4, which is characterized by comprising the following steps:
(1) weighing the raw materials according to the proportion;
(2) pulverizing the raw materials weighed in the step (1) and uniformly mixing to obtain medicinal powder, or extracting with water or/and ethanol according to a conventional method to obtain an extract;
(3) and (3) adding common auxiliary materials or auxiliary components into the medicinal powder or the extract obtained in the step (2) to prepare a preparation.
8. Use of the Chinese medicinal composition according to any one of claims 1 to 3 in the preparation of food, health care products or medicaments for treating and preventing acute alcoholic liver injury.
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