CN103285230B - Pharmaceutical composition for treating pharyngitis and preparation method thereof - Google Patents
Pharmaceutical composition for treating pharyngitis and preparation method thereof Download PDFInfo
- Publication number
- CN103285230B CN103285230B CN201310234092.5A CN201310234092A CN103285230B CN 103285230 B CN103285230 B CN 103285230B CN 201310234092 A CN201310234092 A CN 201310234092A CN 103285230 B CN103285230 B CN 103285230B
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- medicine composition
- radix
- preparation
- precipitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a traditional Chinese medicine for treating acute attack of acute pharyngitis or chronic pharyngitis. The traditional Chinese medicine is prepared from rehmannia, radix ophiopogonis, radix scrophulariae, honeysuckle, fructus forsythia, scutellaria baicalensis, perfoliote knotweed herb, cortex moutan radicis, belamcanda chinensis, radix glehniae, platycodon grandiflorum and liquorice. The traditional Chinese medicine composition is anti-inflammatory, antifebrile, antalgic, antibacterial and antiviral and/or strengthens immune function, can be used for treating or relieving pharyngalgia, dry throat and cauma and/or dysphagia, and also can be applied to a medicine for preventing or treating cold, cough, sphagitis and/or amygdalitis. The invention also provides a preparation of the traditional Chinese medicine composition, as well as a preparation method and application thereof.
Description
Technical field
The invention belongs to technical field of Chinese medicines, particularly, the present invention relates to be used for the treatment of Chinese medicine composition, preparation of pharyngitis and its preparation method and application etc.
Background technology
Pharyngitis is pharyngeal mucosa, and the inflammation of submucous tissue is often a part for upper respiratory tract infection, and the general course of disease is tediously long, obstinate difficult, is divided into acute pharyngitis, the large class of chronic pharyngitis two.Acute pharyngitis shows effect repeatedly, and treatment transfers to chronic not in time.Often, because weather sharply changes, daily life is careless, and lung is defended and lost admittedly, and hyperactivity of fire caused by deficiency of YIN, makes lung disorder of function of stomach, pulmonary's deficiency of YIN, and pyretic toxicity is stifling, causes that swelling throat is red, burning pain, foreign body in pharynx sense or stop up sense, even pathogenic heat are flourishing causes the diseases such as body Fa Gaore.
The generation development speed of China's pharyngitis disease is surprising, along with the raising of people's living standard, and the increase of operating pressure, the aggravation of the fierceness of social competition, especially urban air pollution, in the crowd of city, the number of the infected of pharyngitis is by increasing, and there is downward trend at the age.Therefore the prevention of pharyngitis and treatment task are very arduous, and exploitation, for the medicine for the treatment of pharyngitis, meets clinical needs, not only has direct economic benefit, by improving patient health situation, improve population health level and have equally social benefit widely.
Through retrieval, find to comprise with some similar document of Chinese medicine formula of the present invention, Chinese patent application 99125715, it discloses a kind of Chinese medicine composition, it is by Radix Panacis Quinquefolii, Radix Glehniae, Radix Pseudostellariae, Radix Ginseng, Radix Glycyrrhizae, Herb Gynostemmae Pentaphylli, the Radix Astragali, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Rhizoma Atractylodis, Radix Glycyrrhizae Preparata, Seem Lablab Album, the Cortex Eucommiae, Herba Schizonepetae, Radix Saposhnikoviae, Radix Angelicae Pubescentis, Rhizoma Et Radix Notopterygii, Rhizoma Gastrodiae, Bombyx Batryticatus, Folium Perillae, Radix Angelicae Dahuricae (Radix Heraclei Scabridi), Herba Ephedrae, angle village (Ramulus Uncariae Cum Uncis), Rhizoma Cimicifugae, Radix Bupleuri, Radix Puerariae, Radix Asteris, Semen Armeniacae Amarum, Fructus Perillae, Rhizoma Cynanchi Stauntonii, Flos Inulae, Folium Eriobotryae, Cortex Mori etc. are made, be used for the treatment of cholelithiasis, Liver and kidney essence and blood body fluid loses, the deficiency of vital energy, yang deficiency, essence and blood loses, constipation, the diseases such as cough,
Chinese patent application 03128371, it discloses a kind of medicine, it passes through Calculus Bovis, Cornu Saigae Tataricae powder, Margarita powder, Borneolum Syntheticum (having another name called borneol), Moschus moschiferous perfume (or spice), Venenum Bufonis, Realgar, Fel Ursi, Rhizoma Coptidis, Radix Glycyrrhizae, Cortex Phellodendri, Radix Scutellariae, Flos Lonicerae, Fructus Forsythiae, Radix Rehmanniae, Radix Astragali, Radix Scrophulariae, Folium Isatidis, Cortex Lycii, Herba Verbenae, Rhizoma Picrorhizae, Radix Stellariae, Radix Bupleuri, Rhizoma Polygoni Cuspidati, Herba Houttuyniae, Radix Scutellariae, Rhizoma Coptidis, real secondary, Cortex Phellodendri, Herba Artemisiae Annuae, Radix Glehniae, Radix Ginseng, Herba Taraxaci, Gypsum Fibrosum, the Radix Stemonae, Fructus Gardeniae is made, for killing SARS virus, the acute lung injury that treatment pulmonary fibrosis causes,
Chinese patent application 201010149941, it discloses a kind of Chinese medicine composition, made by Radix Adenophorae, Radix Glehniae, Radix Changii, Rhizoma Polygonati Odorati, Herba Dendrobii, Radix Ephedrae, Radix Ophiopogonis, Radix Asparagi, Fructus Lycii, Fructus Ligustri Lucidi, Carapax Trionycis, Carapax et Plastrum Testudinis, Fructus Schisandrae Chinensis, Galla Chinensis, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Colla Corii Asini, Magnetitum, Cinnabaris, succinum, Os Draconis, Semen Ziziphi Spinosae, Gypsum Fibrosum, the Rhizoma Anemarrhenae, Radix Puerariae, Radix Trichosanthis, Folium Bambusae, Fructus Gardeniae etc., be used for the treatment of night sweat.
The inventor is through long-term and arduous research and practice, obtain unexpectedly a kind of Chinese medicine composition composition formula, itself and existing formula are distinguished larger, not only can be used for the treatment of safely, efficiently acute pharyngitis or acute episode of chronic pharyngitis, also there is antiinflammatory action, refrigeration function, analgesic activity, antibacterial action, antivirus action and/or strengthen immunity function etc., and can be used for the treatment of or alleviate the patient's condition such as the scorching hot and/or dysphagia of pharyngalgia, dry pharynx, can prevent or treat flu, cough, pharyngolaryngitis and/or tonsillitis.In addition, the inventor has also explored the preparation method of this Chinese medicine composition, in the situation that not affecting curative effect of medication and stability, simplify largely preparation flow, different raw materials is processed simultaneously as far as possible, save the input of potential LPT device, saved production cost, shortened preparation flow.
Summary of the invention
The technical problem to be solved in the present invention is the Chinese medicine composition that provides new, its treatment acute pharyngitis or acute episode of chronic pharyngitis.In addition, the present invention also provides preparation, the preparation method and application etc. based on this Chinese medicine composition.
Particularly, in first aspect, the invention provides the Chinese medicine composition that is used for the treatment of acute pharyngitis or acute episode of chronic pharyngitis, it is characterized in that: this Chinese medicine composition is to be made up of the Radix Rehmanniae of following weight portion, Radix Ophiopogonis, Radix Scrophulariae, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Cortex Moutan, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae:
Preferably the Chinese medicine composition of first aspect present invention is to be made up of Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Cortex Moutan, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and the Radix Glycyrrhizae of following weight portion:
In order to ensure the stable and effective of curative effect, the inventor has found out the criteria of quality evaluation of the Chinese medicine composition of first aspect present invention, although the active component in Chinese medicine composition is very complicated, through a large amount of batches of researchs, find that its Content of Chlorogenic Acid is suitable as quality index very much.Therefore preferably the chlorogenic acid content of the Chinese medicine composition of first aspect present invention is not less than 6mg/g, is more preferably not less than 8.5mg/g.
The inventor is through long-felt, drawn not affecting the treatment, has been convenient to the preparation technology of large-scale production safety in the situation that, wherein can merge or the various extracts of parallel processing, and wherein step is convenient to quality control in addition.Therefore, preferably the Chinese medicine composition of first aspect present invention is prepared by being prepared as follows method:
(1) mixture of pulverizing Flos Lonicerae, Cortex Moutan; Or, Flos Lonicerae, Cortex Moutan are pulverized respectively, then mixed;
(2) Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae are added to moisture and decoct for 1～5 time, collect filtrate and be condensed into clear paste;
(3) clear paste of step (2) gained is added to ethanol precipitation, collect filtrate, concentrate, be dried and be ground into powder;
(4) by the crushed products mix homogeneously of step (1) and step (3) gained; With,
(5) chlorogenic acid content in the mix products of detecting step (4), retains qualified product, makes this Chinese medicine composition.
In further preferred above-mentioned preparation method,
In step (1), pulverizing is to be ground into the fine powder that can cross 80～150 mesh sieves, is preferably and is ground into the fine powder that can cross 100～120 mesh sieves;
In step (2), the gross weight adding water is 5～15 times of institute's decocting herbs amount, is preferably 10 times;
In step (2), decoction is point 2～4 decoctions, preferably divides 3 decoctions;
In step (2), each time decocting is 0.5～3h, and preferably each decocting time is 1～2h;
In step (2), the relative density of clear paste is 1.0～1.5(25 DEG C), be preferably 1.10～1.15(25 DEG C);
In step (3), when precipitation, adding ethanol to concentration of alcohol is 60～80%(V/V), be preferably 70%(V/V);
In step (3), the temperature of precipitation is 0～10 DEG C, is preferably 4 DEG C;
In step (3), the time of precipitation is 12～36h, is preferably 24h;
In step (3), it is 1.30～1.35(45 DEG C that simmer down to is concentrated into relative density) thick paste; And/or,
In step (5), the chlorogenic acid content of qualified product is not less than 6mg/g, is not less than 8.5mg/g.
In second aspect, the invention provides the preparation method of the Chinese medicine composition of first aspect present invention, it comprises:
(1) mixture of pulverizing Flos Lonicerae, Cortex Moutan; Or, Flos Lonicerae, Cortex Moutan are pulverized respectively, then mixed;
(2) Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae are added to moisture and decoct for 1～5 time, collect filtrate and be condensed into clear paste;
(3) clear paste of step (2) gained is added to ethanol precipitation, collect filtrate, concentrate, be dried and be ground into powder;
(4) by the crushed products mix homogeneously of step (1) and step (3) gained; With,
(5) chlorogenic acid content in the mix products of detecting step (4), retains qualified product, makes this Chinese medicine composition.
In the preparation method of preferred second aspect present invention,
In step (1), pulverizing is to be ground into the fine powder that can cross 80～150 mesh sieves, is preferably and is ground into the fine powder that can cross 100～120 mesh sieves;
In step (2), the gross weight adding water is 5～15 times of institute's decocting herbs amount, is preferably 10 times;
In step (2), decoction is point 2～4 decoctions, preferably divides 3 decoctions;
In step (2), each time decocting is 0.5～3h, and preferably each decocting time is 1～2h;
In step (2), the relative density of clear paste is 1.0～1.5(25 DEG C), be preferably 1.10～1.15(25 DEG C);
In step (3), when precipitation, adding ethanol to concentration of alcohol is 60～80%(V/V), be preferably 70%(V/V);
In step (3), the temperature of precipitation is 0～10 DEG C, is preferably 4 DEG C;
In step (3), the time of precipitation is 12～36h, is preferably 24h;
In step (3), it is 1.30～1.35(45 DEG C that simmer down to is concentrated into relative density) thick paste; And/or,
In step (5), the chlorogenic acid content of qualified product is not less than 6mg/g, is not less than 8.5mg/g.
In the third aspect, the invention provides the Chinese medicine preparation that is used for the treatment of acute pharyngitis or acute episode of chronic pharyngitis, it comprises the Chinese medicine composition of first aspect present invention, and optionally also comprises pharmaceutically acceptable adjuvant.In this article, pharmaceutically acceptable adjuvant comprises pharmaceutically acceptable carrier, excipient, diluent etc., and they are compatible with active component.Using pharmaceutically acceptable adjuvant useful in preparing drug formulations is known for those of ordinary skills.Chinese medicine preparation of the present invention is combined active component (Chinese medicine composition) and pharmaceutically acceptable adjuvant, is mixed with various preparations, is preferably solid preparation and liquid preparation, more preferably solid preparation.Preparation of the present invention can be unit dosage form, as dosage form and various slow release formulations such as tablet, pill, capsule (comprise sustained release or delay release releasing pattern), powder, suspensoid, granule, tincture, syrup, emulsion agent, suspensions.Preferably the Chinese medicine preparation of third aspect present invention is oral drug preparation, as granule, tablet, pill and/or capsule, most preferably is pill.
Preferably the Chinese medicine preparation of third aspect present invention does not comprise pharmaceutically acceptable adjuvant in addition, is made up of the Chinese medicine composition of first aspect present invention.
The consumption of Chinese medicine preparation can be determined according to the content of active component (Chinese medicine composition) wherein and in conjunction with the situation of concrete dosage form, administering mode and administration object, also can infer people's dosage and determine required content according to zoopery, conventionally not consider pharmaceutically acceptable adjuvant amount wherein.
In fourth aspect, the application of the Chinese medicine composition that the invention provides first aspect present invention in the medicine for the preparation for the treatment of acute pharyngitis or acute episode of chronic pharyngitis.
Aspect the 5th, the Chinese medicine composition that the invention provides first aspect present invention has antiinflammatory action, refrigeration function, analgesic activity, antibacterial action, antivirus action and/or strengthens the application in the medicine of immunity function in preparation.
Aspect the 6th, the Chinese medicine composition that the invention provides first aspect present invention is for the preparation for the treatment of or alleviate the application in the medicine of the scorching hot and/or dysphagia of pharyngalgia, dry pharynx.
Aspect the 7th, the invention provides the Chinese medicine composition of first aspect present invention in the application for the preparation of in prevention or treatment flu, cough, pharyngolaryngitis and/or tonsillitic medicine.
The application of the present invention fourth, fifth, six and seven aspects, has all obtained the support of the experiment that the specific embodiment of the invention provides.Preferably, in the application aspect the present invention fourth, fifth, six and seven, medicine is the Chinese medicine preparation of third aspect present invention.
The excellent effect obtaining of the present invention comprises: determined curative effect and efficient, thus can effectively treat various diseases and the patient's condition including acute pharyngitis or acute episode of chronic pharyngitis etc.; Steady quality, safe and reliable, taking dose is little, untoward reaction is few, and this supervision of drug safety for pay attention to day by day and the public, concerning the prescription of medicine, are highly profitable; Production stage is simple, and product quality monitoring is convenient, can walk abreast or merge and process various crude drugs and extract thereof, and agents useful for same, without special solvent, had therefore both been saved the time, had also reduced production cost, can meet the requirement of large-scale production.
For the ease of understanding, below will be described in detail the present invention by specific embodiment.It needs to be noted, these descriptions are only exemplary descriptions, do not form limitation of the scope of the invention.According to the discussion of this description, many variations of the present invention, change are all apparent concerning one of ordinary skill in the art.
In addition, the present invention has quoted open source literature, and these documents are in order more clearly to describe the present invention, and their full text content is all included in and carried out reference herein, just looks like that repeated description is excessively the same in this article for their full text.
Detailed description of the invention
Further illustrate by the following examples content of the present invention.As do not specialize, the conventional means that in embodiment, technological means used is well known to those skilled in the art and commercially available common instrument, can be referring to relevant regulations and the guide etc. of " Chinese Pharmacopoeia " and SFDA.
The preparation of the anti-sense sore-throat relieving of embodiment 1 ball
1, prescription:
2, the preparation of Chinese medicine composition:
The 12 taste crude drugs of getting above prescription, wherein, Flos Lonicerae, Cortex Moutan are ground into fine powder, and sieve (100～120 order), mixes; After Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Radix Glehniae, Fructus Forsythiae, Radix Scutellariae, Rhizoma Belamcandae, Radix Platycodonis, Radix Glycyrrhizae, Herba Polygoni cymosi mix, 4 times of water gagings that add their gross weight decoct 2 hours in 100 DEG C, collect filtrate, filtering residue adds 3 times of water gagings to be continued to decoct 2 hours, collect filtrate, filtering residue adds 3 times of water gagings to be continued to decoct 1 hour, collects filtrate.Merging filtrate, be concentrated into relative density 1.10～1.15(25 DEG C) clear paste, adding ethanol makes ethanol content reach 70%(V/V), leave standstill 24 hours in 4 DEG C, collect filtrate, in 40～50 DEG C evaporation reclaim ethanol and be concentrated into relative density 1.30～1.35(45 DEG C) thick paste, then dry, pulverize into fine powder and be extract fine powder.The fine powder that extract fine powder and Flos Lonicerae, Cortex Moutan are pulverized mixes, and with appropriate water pill, is dried in 60～80 DEG C, and polishing, makes pill 1000g altogether, obtains anti-sense sore-throat relieving ball.
3, the qualification of drug quality:
Get pill 0.24g, porphyrize, accurately weighed, put in the brown measuring bottle of 50ml, add 50% methanol 30ml, supersound extraction 30 minutes (power 100w, frequency 40Hz), lets cool, be diluted to scale with 50% methanol again, shake up, 0.45 μ m filtering with microporous membrane obtains detection sample, carries out HPLC detection (taking octadecylsilane chemically bonded silica as filler; Taking Yi Jing ?0.4% phosphoric acid (V:V=15:85) as mobile phase; Detect wavelength: 327nm, flow velocity is 1.0ml/min; Taking containing 50% methanol solution of chlorogenic acid 50 μ g/ml as contrast) detect and obtain pill Content of Chlorogenic Acid and be not less than 8.5mg/g.Do not meet discarding of this quality index.
The pharmacodynamic experiment of embodiment 2 Chinese medicine compositions
[tested medicine]
The Chinese medicine composition that the mode that test drug title: embodiment 1 records obtains---anti-sense sore-throat relieving ball (below as without contrary indicate, be called for short " Chinese medicine composition "), wherein every gram of Chinese medicine composition is equivalent to 2.56g crude drug.
[positive control drug]
Aspirin tablet, Hefei Jiu Lian pharmaceutical Co. Ltd, specification: 0.3g/ sheet, lot number: 20040513.
LIYANLING PIAN, Changchun the present Pharmaceutical (group) company limited, specification: 0.5g/ sheet, lot number: 20040501.
Moroxydine, pharmaceutical factory of Fushun City, specification: 0.1g/ sheet, lot number: 20050113.
[experimental animal]
Kunming mouse and Wistar rat: purchased from high-new medical experiment zooscopy center, Changchun, the quality certification number: SCXK (Ji) 2003 ?0004.
Enjoy 9 days Embryo Gallus domesticus of chicken fertilization: purchased from Changchun institute of Biological Products.
[strain]
Streptococcus pneumoniae, beta hemolytic streptococcus, staphylococcus aureus (ATCC25925), bacillus pyocyaneus, influenza virus: A1 type.Above pathogenic bacteria and Strain are all purchased from pathogenic microorganism teaching and research room of preclinical medicine institute of Jilin University controlled use.
[test method and result]:
One, antiinflammatory experiment
1, the impact on rat paw edema due to Ovum Gallus domesticus album
Get 50 of healthy Wister rats, male and female half and half, body weight 160 ?200g.Be divided at random 5 groups, 10 every group, 1. matched group (pure water 10ml/kg); 2. aspirin group 0.4g/kg; 3. Chinese medicine composition high dose group 3g/kg; 4. dosage group 2g/kg in Chinese medicine composition; 5. Chinese medicine composition low dose group 1g/kg.By above each group dosage gastric infusion, successive administration 7 days.Ovum Gallus domesticus album solution 0.1ml/ with right back sufficient plantar subcutaneous injection 10% only causes inflammation, respectively cause scorching before and cause after scorching play 30min, 60min, 120min, 240min adopt the right back sufficient sole of the foot volume-variation of volumetric method measurement, so that the right back sufficient volume-variation before and after scorching is swelling, and calculate swelling percentage rate and inhibitory rate of intumesce by following formula, swelling rate and suppression ratio more on the same group.The each administration group of experimental result and model control group comparative result are in table 1.
Scorching front foot sole of the foot volume × 100% of swelling rate (%)=(cause scorching metapedes sole of the foot Rong Ji – and cause scorching front foot sole of the foot volume)/cause
Suppression ratio (%)=(the average Zhong Zhang of matched group Shuai the average swelling rate of – administration group) average swelling rate × 100% of/matched group
Experimental result: 10% Ovum Gallus domesticus album 0.1ml/ only gives Rat Right metapedes plantar subcutaneous injection preparation experiment swelling model, after Chinese medicine composition administration, can obviously reduce Ovum Gallus domesticus album inflammatory and cause foot swelling, heavy dose of and middle dose effect is more obvious, relatively there is significant difference (P < 0.05, P<0.001) with model control group.Show that Chinese medicine composition improves significantly to Ovum Gallus domesticus album inflammatory foot swelling model.
The impact of table 1 Chinese medicine composition on normal rat paw edema rate and suppression ratio
With matched group comparison: * P<0.05; * P<0.01; * * P<0.001.
2, the impact that Dichlorodiphenyl Acetate induced mice abdominal cavity capillary permeability increases
Get 50 of kunming mices, male and female half and half, body weight 20 ?22 grams, be divided at random 5 groups, 10 every group.1. Normal group (pure water 20ml/kg); 2. the Chinese medicine composition suspension 20ml/kg of Chinese medicine composition high dose group 6g/kg(30%); 3. the Chinese medicine composition suspension 20ml/kg of dosage group 4g/kg(20% in Chinese medicine composition); 4. the Chinese medicine composition suspension 20ml/kg of Chinese medicine composition low dose group 2g/kg(10%); 5. the suspension 20ml/kg of LIYANLING PIAN matched group 4g/kg(20%).By above each group dosage gastric infusion, after 7 days last administration 30min of successive administration, mice is all through the Evans blue normal saline solution 0.1ml/10g of tail vein injection 0.5% body weight, the acetum 0.2ml/ of lumbar injection 0.6% only immediately, after 20min, vertebral pulling is put to death, cut off abdominal cavity, divide and wash abdominal cavity sucking-off cleaning mixture for several times with 6ml normal saline.After merging cleaning mixture, add normal saline to 10ml, 3000 revs/min of centrifugal 15min get supernatant and carry out colorimetric in wavelength 590nm place, measure animal abdominal cavity cleaning mixture optical density value.The each administration group of experimental result and model control group comparison, between group, t inspection is processed, and the results are shown in Table 2.
Experimental result: 0.6% acetum 0.2ml/ mouse peritoneal injection can be prepared the model that mouse peritoneal capillary permeability increases, the mouse peritoneal capillary permeability that Chinese medicine composition administration can significantly suppress due to acetic acid increases, high dose effect is more obvious, relatively has significant difference (P < 0.01) with model control group.Show that the mouse peritoneal capillary permeability increase due to Chinese medicine composition Dichlorodiphenyl Acetate has obvious inhibitory action.
The inhibitory action that table 2 Chinese medicine composition increases capillary permeability due to mice acetic acid.
With matched group comparison: * P<0.05; * P<0.01.
Two, antipyretic experiment
1, the impact that due to induced by endotoxin, rat fever model body temperature changes
Get 50 of normal rats, male and female half and half, body weight 200 ?220g, tested rat is carried out to body temperature measurement, METHOD FOR CONTINUOUS DETERMINATION 3 times, the body temperature person of stablizing is for test.Be divided at random 5 groups, 10 every group, 1. Chinese medicine composition high dose group 3g/kg; 2. dosage group 2g/kg in Chinese medicine composition; 3. Chinese medicine composition low dose group 1g/kg; 4. aspirin group 0.4g/kg; 5. matched group, same volume pure water.By above each group dosage gastric infusion, successive administration 3 days, after last administration, 30min is through tail vein injection 50EU/kg escherichia coli endotoxin.At interval of 30min measure a body temperature, after 120min every 60min take temperature once, Continuous Observation 360 minutes.The body temperature of more each treated animal on different time points changes.Each group data are added and subtracted standard deviation with mean and are represented, between group, t inspection is processed, and the results are shown in Table 3.
Experimental result: 50EU/kg escherichia coli endotoxin tail vein injection causes rat fever model test, after Chinese medicine composition administration, can significantly reduce the lift-off value of the body temperature of rat due to endotoxin, heavy dose of and middle dose effect is more obvious, relatively there is significant difference (P<0.05 with model control group, P<0.01), show that rat temperature due to Chinese medicine composition induced by endotoxin raises and has obvious inhibitory action.
2, the impact that due to induced by endotoxin, fever in rabbits animal model body temperature changes
Get 40 of the qualified rabbit of body temperature, male and female half and half, body weight 2 ?3kg.Be divided at random 5 groups, 8 every group, 1. Chinese medicine composition high dose group 2g/kg; 2. dosage group 1g/kg in Chinese medicine composition; 3. Chinese medicine composition low dose group 0.5g/kg; 4. aspirin group 0.3g/kg; 5. matched group, same volume pure water.By above each group dosage gastric infusion, successive administration 3 days, after last administration, 30min is through auricular vein injection 5EU/kg escherichia coli endotoxin, and once, after 120min, interval 60min measures once interval 30min take temperature, Continuous Observation 360min.The body temperature of more each treated animal on different time points changes.Each group data are added and subtracted standard deviation with mean and are represented, between group, t inspection is processed, and the results are shown in Table 4.
Experimental result: 5EU/kg escherichia coli endotoxin auricular vein is injected to prepare and caused fever in rabbits animal model, Chinese medicine composition can significantly reduce the body temperature of fever in rabbit due to 5EU/kg escherichia coli endotoxin, heavy dose of and middle dose effect is more obvious, relatively there is significant difference (P<0.05 with model control group, P<0.01), show, the rabbit body temperature due to Chinese medicine composition induced by endotoxin raises obvious inhibitory action.
The comparison that rat fever model body temperature due to table 3 drug composition induced by endotoxin changes
With matched group comparison: * P<0.05; * P<0.01; * * P<0.001.
The comparison that table 4 drug composition changes rabbit fever models body temperature
With matched group comparison: * P<0.05; * P<0.01; * * P<0.001.
Three, analgesic experiment
1, writhing method
Get 50 of kunming mices, male and female half and half, body weight 18 ?22g, raise 3 days at laboratory adaptability, be divided at random 5 groups, 10 every group.1. matched group, pure water 20ml/kg; 2. aspirin group 0.6g/kg; 3. Chinese medicine composition low dose group 2g/kg; 4. dosage group 4g/kg in Chinese medicine composition; 5. Chinese medicine composition high dose group 6g/kg.The equal gastric infusion of each group, every day 1 time, successive administration 5 days.Last administration 30min pneumoretroperitoneum is injected 0.7% acetum 0.1ml/10g, after observed and recorded gives acetic acid, the interior mouse writhing of 10min reacts (mouse web portion pastes ground, indent, stretching, extension hind leg, distortion) number of times, more each administration group and pure water group experimental result, between group, t inspection is processed, and the results are shown in Table 5.
From table 5, the mouse writhing reaction that Chinese medicine composition Dichlorodiphenyl Acetate stimulates has certain effect that alleviates, and relatively has significant difference (P<0.01 or P<0.001) with pure water injection group.
The impact of table 5 Chinese medicine composition on mouse writhing number of times
With matched group comparison: * * P < 0.01; * * P < 0.001.
2, tenderness method
Get 50 of qualified rats, male and female half and half, body weight 160～200g, is divided into 5 groups at random, 10 every group.1. matched group, pure water 10ml/kg; 2. aspirin group 0.4g/kg; 3. Chinese medicine composition low dose group 1g/kg; 4. dosage group 2g/kg in Chinese medicine composition; 5. Chinese medicine composition high dose group 3g/kg.The equal gastric infusion of each group, every day 1 time, successive administration 5 days.After the administration phase finishes, adopt YLS ?3E tenderness instrument measure respectively rat pain threshold when 30min, 60min and 120min after administration.Relatively different dosing group pain threshold situation of change.Between group, t inspection is processed, and the results are shown in Table 6.
From table 6, Chinese medicine composition group has certain inhibitory action to physical factor is pain caused, and relatively there were significant differences (P<0.01 or P<0.001) illustrates that the pain that Chinese medicine composition causes rat physical factor has analgesic activity with matched group.
The impact (tenderness method) of table 6 Chinese medicine composition on animal pain threshold
(N=10)
With matched group comparison: * P < 0.05; * P < 0.01; * * P < 0.001.
The laboratory observation to Chinese medicine composition analgesic activity by above-mentioned writhing method and two kinds of analgesic activity experimental techniques of analgesia method, shows that Chinese medicine composition has obvious analgesic activity, increases analgesic activity as seen strengthen to some extent in experiment with dosage.
Four, antibacterial, antivirus action experiment
(1) antivirus action experiment
1, interior resisting virus effect experiment
Get 60 of kunming mices, male and female half and half, body weight 18 ?20 grams.Laboratory animal is divided into 6 groups at random, 10 every group.1. influenza virus matched group, pure water 20ml/kg; 2. 1 group of 25mg/kg of moroxydine matched group; 3. 2 groups of 12.5mg/kg of moroxydine matched group; 4. 1 group of 6g/kg in Chinese medicine composition; 5. 2 groups of 4g/kg of Chinese medicine composition.6. 3 groups of 2g/kg of Chinese medicine composition.By the above each continuous gastric infusion of dosage 3 days of organizing, after last administration, 4h respectively organizes mice per nasal under ether light anaesthesia and splashes into influenza virus, and 30 μ l/ are (A1 type, hemagglutinative titer 1:240) only.Continuous Observation animal morbidity in 7 days and death condition, and record result.The results detailed in Table 7.
All be starkly lower than negative control group through the mortality rate of Chinese medicine composition administration animal as seen by table 7, increase fatality rate with dosage and reduce more obvious.Show that Chinese medicine composition can obviously reduce the mortality rate of influenza infection mice, has significant protective effect to influenza virus infection animal.
The impact of table 7 Chinese medicine composition infected by influenza infection animal fatality rate
2, external infected by influenza effect experiment
Get 28 pieces of normotrophic Embryo Gallus domesticus (being fertilized 9 days), Embryo Gallus domesticus is divided into 7 groups at random, 4 pieces every group.Specifically be grouped as follows: three concentration of positive control medicine moroxydine (1mg/ml, 0.5mg/ml, 0.25mg/ml) are divided three groups, three concentration of trial drug Chinese medicine composition (2.2mg/ml, 1.1mg/ml, 0.55mg/ml) are divided three groups.Separately establish virus control group (concentration).Embryo Gallus domesticus is fetched and observed Embryo Gallus domesticus growth and draw air chamber position after whether normal under ovoscopy lamp, other without aperture of trunk place vertebra at air chamber with aseptic vertebra after sterilization, by tested variable concentrations medicine and positive control medicine, inject respectively in allantoic cavity 0.1ml/ piece (matched group injection physiological saline solution), aperture is sealed.36 DEG C are continued to cultivate 48h.Observe Embryo Gallus domesticus growing state next day and inject influenza virus 0.1ml/ piece.Continue to cultivate.Observe Embryo Gallus domesticus growing state every day, Embryo Gallus domesticus dead within 24h is abandoned it.Cultivate and take out afterwards for three days, put 4 DEG C of refrigerators.Get allantoic fluid next day, detect influenza virus by ball agglutination test method and tire.
Experimental result: three concentration of Chinese medicine composition as shown in table 8 all have anti-viral effect in various degree, and it is more obvious to increase anti-viral effect with dosage.
The impact that the external infected by influenza of table 8 Chinese medicine composition is tired
(2) antibacterial action
Get 50 of kunming mices, male and female half and half, body weight 18 ?20 grams.Observe and be divided at random afterwards 5 groups in three days, 10 every group.1 ?3 groups of three variable concentrations (6gkg that are tested Chinese medicine composition
?1, 4gkg
?1, 2gkg
?1), 4 groups is the contrast of LIYANLING PIAN positive drug, 5 groups is antibacterial negative control.In first three day of bacterial infection, except antibacterial matched group, other 4 groups all by different pharmaceutical and variable concentrations gastric infusion (20mlkg
?1).Negative control group gives same volume pure water.After last administration 4h, the 100% lethal dose staphylococcus aureus bacterium liquid 0.5ml/ having measured through lumbar injection only.Observe the mortality rate (%) that infects latter 7 days mices.
Experimental result: as shown in table 9.Chinese medicine composition can obviously reduce the fatality rate of mouse infection staphylococcus aureus, shows that Chinese medicine composition has certain protective effect to lethal dose infection of staphylococcus aureus mice.
The protective effect of table 9 Chinese medicine composition to lethal dose infection of staphylococcus aureus mice
Five, immunoregulation effect experiment
(1) specific immunity experiment
1, on the allergic impact of dinitrochlorobenzene induced mice Delayed onset
Get 50 of kunming mices, male and female half and half, body weight 20 ?24 grams.Be divided at random 5 groups, 10 every group, labelling, weigh, record body weight.1. matched group, pure water 20ml/kg; 2. positive controls, LIYANLING PIAN matched group 4g/kg; 3. Chinese medicine composition low dose group 2g/kg; 4. dosage group 4g/kg in Chinese medicine composition; 5. Chinese medicine composition high dose group 6g/kg.The Er Xiao base Lv Ben of all animal via subcutaneous injections 1.25% ?acetone soln, 0.02ml/ only, sensitization.24h after sensitization, starts the medicine corresponding by each group and dosage gastric infusion, once a day, and successive administration 9 days.The 10th day with 1.25% Er Xiao base Lv Ben ?acetone soln, 0.02ml/ only inject with the right foot pad of mice in the middle of, left foot injection is with dosage acetone soln.After 38h, put to death animal, cut biped from ankle, take respectively weight with electronic balance.The relatively difference of two heavy sensation in the foot amounts, as paw swelling, between group, t inspection is processed, and the results are shown in Table 10.
Experimental result: as shown in table 10, high dose group and middle dosage group mice foot swelling value, all apparently higher than matched group (P < 0.05), show that reaction has certain facilitation to Chinese medicine composition to animal cell immunity.
Table 10 Chinese medicine composition is on the allergic impact of dinitrochlorobenzene induced mice Delayed onset
With matched group comparison: * P < 0.05.
2, the impact on animal body fluid immunologic function (hemolytic antibody method)
(1) laboratory animal and grouping:
Get 50 of kunming mices, body weight 20 ?24 grams, male and female half and half.Be divided at random 5 groups, 10 every group.
(2) test method and step
1. the preparation of chicken erythrocyte suspension: venous blood sampling 1ml under the chicken wing.With normal saline Washed Red Blood Cells three times, then chicken red blood cell is configured to 5% suspension for subsequent use.
2. the preparation of GPC: Cavia porcellus is through heart extracting blood, and the centrifugal 20min of 3000rpm/min, gets serum, by normal saline 1:10 diluted for use for serum.
(3) medication and dosage
Each group mice respectively through the chicken red blood cell suspension 0.2ml/ of lumbar injection 5% only.Then start administration, dosage is respectively: 1 group of 6g/kg of Chinese medicine composition; 2 groups of 4g/kg of Chinese medicine composition; 3 groups of 2g/kg of Chinese medicine composition; Positive controls, LIYANLING PIAN 4g/kg; Normal group, pure water 20ml/kg.
Successive administration 10 days, administration expires, and wins eyeball of mouse and gets blood, and the blood of getting adds in 0.5ml centrifuge tube, and the centrifugal 15min of 3000rpm/min gets 100 times of serum normal saline dilutions, for subsequent use.
(4) method of testing
Get 50, test tube, numbering is put into test tube rack.Get respectively each mice serum 1ml and add test tube.In every test tube, add respectively 5% Sanguis Gallus domesticus red cell suspension 0.5ml.In every test tube, add respectively 10% guinea pig serum 0.5ml.After application of sample completes, all test tubes are put into 37 DEG C of calorstats and cultivate 30min.After finishing, cultivation puts into refrigerator (0 DEG C) stopped reaction.Test tube is put into centrifuge, and the centrifugal 20min of 3000rpm/min, gets supernatant.Use 7550 spectrophotometers, under wavelength 540nm not heal optical density (OD) value of final proof as the each sample of blank determination.
Experimental result: have no significant difference with matched group comparison Chinese medicine composition administration treated animal serum OD value and matched group, show that the Chinese medicine composition of test dose affects without significance normal animal body fluid immunologic function.Concrete outcome is as table 11.
The impact of table 11 Chinese medicine composition on animal body fluid immunologic function
(2) nonspecific immunity experiment
The impact of non-mice reticuloendothelial system phagocytic function
Get 50 of kunming mices, male and female half and half, body weight 18 ?20 grams, be divided at random 5 groups, 10 every group.1. Normal group, pure water 20ml/kg; 2. positive controls, LIYANLING PIAN matched group 4g/kg; 3. Chinese medicine composition low dose group 2g/kg; 4. dosage group 4g/kg in Chinese medicine composition; 5. Chinese medicine composition high dose group 6g/kg.By each group of corresponding medicine and dosage gastric infusion, continuous 7 days once a day, after last administration, 30min was through tail vein injection india ink 0.05ml/10g; After injection, 1min, 5min get blood 20ul from vena orbitalis posterior respectively with suction pipe.Be dissolved in the Na of 2ml0.1%
2cO
3in solution, shake up.With 7550 preheated spectrophotometers under 675nm, with 0.1% Na
2cO
3solution does the each sample optical density value of blank determination (OD value), and calculates phagocytic index K and activate the phagocytic capacity α, and between group, t inspection is processed.
Experimental result: Chinese medicine composition administration treated animal phagocyte phagocytic index and matched group relatively have significant difference (P < 0.05,0.01 > P), activate the phagocytic capacity obviously improves (P < 0.05,0.01 > P), shows that Chinese medicine composition has certain facilitation to animal mononuclear phagocyte phagocytic function.Concrete outcome is in table 12.
The impact of table 12 Chinese medicine composition on nonspecific immunity of mice
With matched group comparison: * P<0.05; * P<0.01.
[experiment conclusion]:
1. due to pair Ovum Gallus domesticus album, rat inflammatory foot swelling model improves significantly, and the capillary permeability increase of Dichlorodiphenyl Acetate induced mice abdominal cavity has obvious inhibitory action, shows, Chinese medicine composition has certain antiinflammatory action.
2. due to induced by endotoxin, rat, the rising of rabbit fever models animal heat have obvious inhibitory action, show, Chinese medicine composition has refrigeration function.
3. pair chemical factor causes the pain of pain and the pain that physical factor causes pain is all had to certain inhibitory action, shows, Chinese medicine composition has certain analgesic activity.
4. aspect immunomodulating, animal cell immunity function and mononuclear cell phagocytic function are had to certain facilitation, not obvious to animal body fluid Immune Function.
5. extracorporeal antivirus effect result of the test shows that Chinese medicine composition can improve tiring of resisiting influenza virus; Antiviral assay in vivo shows, Chinese medicine composition obviously reduces the mortality rate of influenza infection mice, and prompting Chinese medicine composition infected by influenza has good inhibitory action.
6. in body, bacteriostatic test shows that Chinese medicine composition can obviously reduce the mortality rate of fatal dose infection of staphylococcus aureus mice, illustrates, Chinese medicine composition has antibacterial action.
In sum, this Chinese medicine composition has the effect of heat-clearing and toxic substances removing, antiinflammatory, pain relieving and adjusting body's immunity.
The long-term toxicity test for animals of embodiment 3 Chinese medicine compositions
Use Wistar rat, give respectively through gavage the Chinese medicine composition 5.25gkg that in embodiment 1 prepared by method for twice on the one
?1body weight (is amounted to crude drug amount: 13.44gkg
?1body weight), 3.75gkg
?1body weight (is amounted to crude drug amount: 9.6gkg
?1body weight), 2.25gkg
?1body weight (is amounted to crude drug amount: 5.76gkg
?1body weight).Owing to being subject to the restriction of administration concentration and administration volume, dosage cannot increase.Every day, dosage was equivalent to respectively clinical dosage (0.21gkg for plan
?1body weight) 50 times, 35.7 times and 21.4 times, continuously gastric infusion 12 weeks, Reversible observe 2 weeks.
Result shows, under the dosage that is equivalent to 50 times of clinical plan dosage, 35.7 times and 21.4 times, administration is observed 12 weeks and Reversible observe 2 weeks, and animal subject has no the toxic reaction relevant to drug toxicity, shows that this Chinese medicine composition oral administration safety is good.
Claims (16)
1. the Chinese medicine composition that is used for the treatment of acute pharyngitis or acute episode of chronic pharyngitis, is characterized in that: this Chinese medicine composition is to be made up of the Radix Rehmanniae of following weight portion, Radix Ophiopogonis, Radix Scrophulariae, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Cortex Moutan, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae:
Wherein the chlorogenic acid content of this Chinese medicine composition is not less than 6mg/g.
2. Chinese medicine composition claimed in claim 1, is characterized in that: it is to be made up of the Radix Rehmanniae of following weight portion, Radix Ophiopogonis, Radix Scrophulariae, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Cortex Moutan, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae:
3. Chinese medicine composition claimed in claim 1, is characterized in that: wherein the chlorogenic acid content of this Chinese medicine composition is not less than 8.5mg/g.
4. Chinese medicine composition claimed in claim 1, is characterized in that: it is by being prepared as follows method preparation:
(1) mixture of pulverizing Flos Lonicerae, Cortex Moutan; Or, Flos Lonicerae, Cortex Moutan are pulverized respectively, then mixed;
(2) Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae are added to moisture and decoct for 1～5 time, collect filtrate and be condensed into clear paste;
(3) clear paste of step (2) gained is added to ethanol precipitation, collect filtrate, concentrate, be dried and be ground into powder;
(4) by the crushed products mix homogeneously of step (1) and step (3) gained;
(5) chlorogenic acid content in the mix products of detecting step (4), retains qualified product, and the chlorogenic acid content of qualified product is not less than 6mg/g, makes this Chinese medicine composition.
5. Chinese medicine composition claimed in claim 4, is characterized in that: wherein,
In step (1), pulverizing is to be ground into the fine powder that can cross 80～150 mesh sieves;
In step (2), the gross weight adding water is 5～15 times of institute's decocting herbs amount;
In step (2), decoction is point 2～4 decoctions;
In step (2), each time decocting is 0.5～3h;
In step (2), the relative density in the time of 25 DEG C of clear paste is 1.0～1.5;
In step (3), when precipitation, adding ethanol to concentration of alcohol is 60～80% (V/V);
In step (3), the temperature of precipitation is 0～10 DEG C;
In step (3), the time of precipitation is 12～36h;
In step (3), be concentrated into the thick paste that the relative density in the time of 45 DEG C is 1.30～1.35;
In step (5), the chlorogenic acid content of qualified product is not less than 8.5mg/g.
6. Chinese medicine composition claimed in claim 5, is characterized in that: wherein,
In step (1), pulverizing is to be ground into the fine powder that can cross 100～120 mesh sieves;
In step (2), the gross weight adding water is 10 times of institute's decocting herbs amount;
In step (2), decoction is point 3 decoctions;
In step (2), each time decocting is 1～2h;
In step (2), the relative density in the time of 25 DEG C of clear paste is 1.10～1.15;
In step (3), when precipitation, adding ethanol to concentration of alcohol is 70% (V/V);
In step (3), the temperature of precipitation is 4 DEG C;
In step (3), the time of precipitation is 24h.
7. the preparation method of Chinese medicine composition claimed in claim 3, is characterized in that: this preparation method is as follows:
(1) mixture of pulverizing Flos Lonicerae, Cortex Moutan; Or, Flos Lonicerae, Cortex Moutan are pulverized respectively, then mixed;
(2) Radix Rehmanniae, Radix Ophiopogonis, Radix Scrophulariae, Fructus Forsythiae, Radix Scutellariae, Herba Polygoni cymosi, Rhizoma Belamcandae, Radix Glehniae, Radix Platycodonis and Radix Glycyrrhizae are added to moisture and decoct for 1～5 time, collect filtrate and be condensed into clear paste;
(3) clear paste of step (2) gained is added to ethanol precipitation, collect filtrate, concentrate, be dried and be ground into powder;
(4) by the crushed products mix homogeneously of step (1) and step (3) gained;
(5) chlorogenic acid content in the mix products of detecting step (4), retains qualified product, and the chlorogenic acid content of qualified product is not less than 8.5mg/g, makes this Chinese medicine composition.
8. the preparation method of Chinese medicine composition claimed in claim 7, is characterized in that: wherein,
In step (1), pulverizing is to be ground into the fine powder that can cross 80～150 mesh sieves;
In step (2), the gross weight adding water is 5～15 times of institute's decocting herbs amount;
In step (2), decoction is point 2～4 decoctions;
In step (2), each time decocting is 0.5～3h;
In step (2), the relative density in the time of 25 DEG C of clear paste is 1.0～1.5;
In step (3), when precipitation, adding ethanol to concentration of alcohol is 60～80% (V/V);
In step (3), the temperature of precipitation is 0～10 DEG C;
In step (3), the time of precipitation is 12～36h;
In step (3), be concentrated into the thick paste that the relative density in the time of 45 DEG C is 1.30～1.35;
In step (5), the chlorogenic acid content of qualified product is not less than 8.5mg/g.
9. the preparation method of Chinese medicine composition claimed in claim 8, is characterized in that: wherein,
In step (1), pulverizing is to be ground into the fine powder that can cross 100～120 mesh sieves;
In step (2), the gross weight adding water is 10 times of institute's decocting herbs amount;
In step (2), decoction is point 3 decoctions;
In step (2), each time decocting is 1～2h;
In step (2), the relative density in the time of 25 DEG C of clear paste is 1.10～1.15;
In step (3), when precipitation, adding ethanol to concentration of alcohol is 70% (V/V);
In step (3), the temperature of precipitation is 4 DEG C;
In step (3), the time of precipitation is 24h.
10. the Chinese medicine preparation that is used for the treatment of acute pharyngitis or acute episode of chronic pharyngitis, is characterized in that: it comprises arbitrary described Chinese medicine composition of claim 1～6, and also comprises pharmaceutically acceptable adjuvant.
11. Chinese medicine preparation claimed in claim 10, is characterized in that: it is oral drug preparation.
Chinese medicine preparation described in 12. claim 11, is characterized in that: it is granule, tablet, syrup, suspensoid, pill or capsule.
13. are used for the treatment of the Chinese medicine preparation of acute pharyngitis or acute episode of chronic pharyngitis, it is characterized in that: it comprises arbitrary described Chinese medicine composition of claim 1～6, and does not comprise pharmaceutically acceptable adjuvant.
The application of arbitrary described Chinese medicine composition of 14. claim 1～6 in the medicine for the preparation for the treatment of acute pharyngitis or acute episode of chronic pharyngitis.
The arbitrary described Chinese medicine composition of 15. claim 1～6 has antiinflammatory action, refrigeration function, analgesic activity, antibacterial action, antivirus action and/or strengthens the application in the medicine of immunity function in preparation.
The arbitrary described Chinese medicine composition of 16. claim 1～6 is for the preparation for the treatment of or alleviate the application in the medicine of the scorching hot and/or dysphagia of pharyngalgia, dry pharynx.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310234092.5A CN103285230B (en) | 2013-06-13 | 2013-06-13 | Pharmaceutical composition for treating pharyngitis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310234092.5A CN103285230B (en) | 2013-06-13 | 2013-06-13 | Pharmaceutical composition for treating pharyngitis and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103285230A CN103285230A (en) | 2013-09-11 |
| CN103285230B true CN103285230B (en) | 2014-09-10 |
Family
ID=49087119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310234092.5A Active CN103285230B (en) | 2013-06-13 | 2013-06-13 | Pharmaceutical composition for treating pharyngitis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103285230B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103908580B (en) * | 2014-04-21 | 2016-03-09 | 李文东 | One treats tonsillitic Traditional Chinese medicine lozenge and preparation method |
| CN104258231A (en) * | 2014-10-09 | 2015-01-07 | 王淑强 | Traditional Chinese medicine composition for treating cough |
| CN111991484A (en) * | 2020-09-18 | 2020-11-27 | 天津中医药大学 | A pharmaceutical composition for treating respiratory diseases in winter |
| CN116236532B (en) * | 2022-12-28 | 2024-05-03 | 天津现代创新中药科技有限公司 | Medicinal and edible composition for preventing and/or treating pharyngitis and related diseases, and preparation method and application thereof |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895606A (en) * | 2006-06-21 | 2007-01-17 | 贵阳德昌祥药业有限公司 | Heat-clearing and toxicity-relieving Chinese-medicinal preparation with phlegm-resolving and cough-relieving functions and its making method |
| CN102091191B (en) * | 2011-02-21 | 2012-06-20 | 路正平 | Medicament for treating chronic pharyngitis |
| CN102178793A (en) * | 2011-04-08 | 2011-09-14 | 宋榜林 | Chinese medicine preparation for treating tonsillitis and pharyngolaryngitis and preparation method thereof |
| CN102949583A (en) * | 2011-11-08 | 2013-03-06 | 沈阳创达技术交易市场有限公司 | Traditional Chinese preparation for treating cold and taking method for same |
| CN102772649B (en) * | 2012-07-12 | 2015-06-10 | 青岛市市立医院 | Traditional Chinese medicine composition for treating chronic pharyngitis and preparation method thereof |
| CN102973753A (en) * | 2012-10-27 | 2013-03-20 | 李新玲 | Traditional Chinese medicine for treatment of chronic pharyngitis |
| CN102949516A (en) * | 2012-11-14 | 2013-03-06 | 西安福安创意咨询有限责任公司 | Health-care product lozenge for pharyngitis and preparation method thereof |
| CN102961579A (en) * | 2012-11-19 | 2013-03-13 | 青州市经济开发区卫生院 | Traditional Chinese medicine composition for treating chronic pharyngitis |
-
2013
- 2013-06-13 CN CN201310234092.5A patent/CN103285230B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103285230A (en) | 2013-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2754064C (en) | Plant extract compositions for the treatment of influenza and inflammation | |
| CN103285230B (en) | Pharmaceutical composition for treating pharyngitis and preparation method thereof | |
| CN105560372A (en) | Traditional Chinese medicine spray for treating rhinitis and preparation method thereof | |
| CN101411782B (en) | Pharmaceutical composition for treating acute pharyngitis and preparation method thereof | |
| CN101480451B (en) | Prescription for treating upper respiratory tract infection and use thereof | |
| CN101628108B (en) | Traditional Chinese medicinal granules for treating wind-cold evil and preparation method thereof | |
| CN102861255B (en) | A kind ofly treat the medicine of influenza and the preparation method of preparation thereof and method of quality control | |
| CN101468089A (en) | Chinese medicinal composition for diffusing lung, relieving exterior syndrome, suppressing cough and reducing phlegm and preparation method thereof | |
| CN103599495A (en) | Chinese medicinal composition for treatment of hepatitis B | |
| CN105535738A (en) | Traditional Chinese medicine preparation for treating chronic cough and preparing method thereof | |
| CN102357158A (en) | Medicament for preventing and curing avian infectious laryngotracheitis and preparation method thereof | |
| CN103007111B (en) | Chinese medicinal preparation for treating diarrhea of rabbits and preparation method of Chinese medicinal preparation | |
| CN103961614A (en) | Traditional Chinese medicine composition for treating respiratory system diseases as well as preparation method and application thereof | |
| CN103006773A (en) | Traditional Chinese granule for children for clearing heat | |
| CN101274034B (en) | Traditional Chinese medicine composition for relieving exterior syndrome, clearing heat and detoxicating | |
| CN104474021A (en) | Traditional Chinese medicine composition for treatment of exterior heat and preparation method thereof | |
| CN101371905A (en) | Chinese medicine lozenge for treating throat disease | |
| CN104027401B (en) | A kind of for child's antiviral, the SHUANGHUANLIAN drug regimen and preparation method thereof of heat clearing and inflammation relieving | |
| CN102688335B (en) | Chinese medicine composition for treating wind-heat type common cold and preparation method of Chinese medicine composition | |
| CN102139002A (en) | Cortex phellodendri polysaccharide extract and preparation method and medical application thereof | |
| CN102205104B (en) | Compound oral solution for treating infantile cough and preparation method thereof | |
| CN103040939B (en) | Traditional Chinese medicine composition for treating cold, as well as preparation method and application thereof | |
| CN106309554A (en) | Scutellaria baicalensis extractive for preventing and/or treating dental ulcer and drug composition thereof | |
| CN103721058A (en) | Traditional Chinese medicine preparation for preventing influenza viruses | |
| CN100469375C (en) | Medicine for treating hepatitis and its preparing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190708 Address after: 274000 No. 99 Kunming Road, Heze Mudan Industrial Park, Shandong Province Patentee after: SHANDONG DANHONG PHARMACEUTICAL CO., LTD. Address before: 710077 No. 69 Jinye Road, Xi'an High-tech Zone, Shaanxi Province Patentee before: Xi'an Xintong Pharmaceutical Research Co., Ltd. |
|
| TR01 | Transfer of patent right |