CN109288870A - Bombyx batryticatus water extract is preparing the application in anticonvulsant drug - Google Patents
Bombyx batryticatus water extract is preparing the application in anticonvulsant drug Download PDFInfo
- Publication number
- CN109288870A CN109288870A CN201811368712.3A CN201811368712A CN109288870A CN 109288870 A CN109288870 A CN 109288870A CN 201811368712 A CN201811368712 A CN 201811368712A CN 109288870 A CN109288870 A CN 109288870A
- Authority
- CN
- China
- Prior art keywords
- bombyx batryticatus
- water extract
- bombyx
- batryticatus
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/08—Antiepileptics; Anticonvulsants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Insects & Arthropods (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Neurosurgery (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Pain & Pain Management (AREA)
Abstract
It is the bombyx batryticatus to ossify 5 days or more that the invention discloses bombyx batryticatus water extracts preparing the application in anticonvulsant drug, the bombyx batryticatus；Its time of ossifing is calculated since silkworm is susceptible at the point of death.Consider from production and cost of manufacture, the bombyx batryticatus that the bombyx batryticatus preferably ossifys 5-9 days.The bombyx batryticatus water extract is made by following steps: being weighed bombyx batryticatus (powder), is added water heating extracting several times with 1:5-1:10 solid-liquid ratio, each 1h or more is concentrated, freeze-dried acquisition bombyx batryticatus water extract after extracting solution filtering.The present invention has found an important factor for influence bombyx batryticatus water extract anticonvulsant efficacy accurately --- and ossify the time, provide the bombyx batryticatus of drug effect preferably, the more stable rigid time, the standardized production for Chinese medicine bombyx batryticatus provides foundation.
Description
Technical field
The invention belongs to the field of Chinese medicines, and in particular to bombyx batryticatus water extract is preparing the application in anticonvulsant drug.
Background technique
Bombyx batryticatus is that the larva in Bombycidae insect silkworm (Bombyx mori L.) 4~5 ages infects (or artificial infection) white deadlock
Bacterium (Beauveria bassiana (Bals.) Vuillant) and lethal hirudo leech.
Bombyx batryticatus medicinal history is very long, just medicinal about bombyx batryticatus in the Chinese medicine works Shennong's Herbal of the Eastern Han Dynasty
Record, cure mainly " the pediatric epilepsy scared morbid night crying of babies.Three worms are gone, are gone out black dim, it is good to make us complexion, man's yin ulcer disease ", it is to be closed in human history
In the earliest record of entomogenous fungi.
Compendium of Material Medica written by Ming Dynasty's Li Shizhen (1518-1593 A.D.) has carried out comprehensive summary to bombyx batryticatus, it is claimed to cure mainly " relieve heat disease tuberculosis scrofula
Scrofula, intermittent headache, wind worm dentalgia, skin wind sore, erysipelas are had an itch, phlegm malaria crux, married woman's alactation, blood under metrorrhagia, and children's malaria loses squama
Body, all metal-inflicted wounds treat moon apoplexy hemorrhoid ".In addition, " haigoushen ", " Bencao Tujing ", " Japan hanako materia medica ", " medicine origin ", " this
Grass is looked for the truth " and the ancient Chinese medicines pharmacopeia nationality such as " beautiful Chinese catalpa medicine solution " in also have bombyx batryticatus effect and medicinal record.
Currently, the clinical application of bombyx batryticatus is also very extensive, is mainly used for treating infantile convulsion, epilepsy, apoplexy, fever head
Bitterly, the diseases such as coughing with a lot of sputum.There are 49 kinds in existing 513 kinds of people's patent medicine containing bombyx batryticatus, has 29 containing bombyx batryticatus in 64 kinds of paediatrics patent medicine
Kind.Hospital's formula dosage, which is about 60, to be shown to part institute of traditional Chinese medicine medicine materical crude slice utility efficiency, the amount of money comes first 15, in animal
It comes out at the top in class medicine materical crude slice.
It is had recorded altogether in " Chinese Pharmacopoeia ", " national standard for traditional Chinese medicines ", " Traditional Chinese medicine historical preparation " and " new drug become a full member standard "
175 Chinese medicine prescriptions containing bombyx batryticatus, such as: curing mainly " the seven precious balls " of arresting convulsion slit phlegm, disperse accumulation defaecation, heat-clearing is relieving convulsion, relieve heat
" Infantile Niuhuang Powder " of phlegm, dispelling pathogenic wind and eliminating phlegm determine epilepsy and stop " Yixian pill " etc. jerked.Take a broad view of Chinese medicinal formulae at all times, clinical function and
From the point of view of curing mainly disease, for bombyx batryticatus as calming the liver to stop the wind medicinal material, primary efficacy is " dispelling wind and relieving convulsion, reducing phlegm and resolving masses ".
In addition, there are many character for influencing Bombyx Batryticatus extract anticonvulsant efficacy, industry fails to hold to its effect of influence at present
Principal element, cause the irregular of the anticonvulsion drug effect of Bombyx Batryticatus extract, affect the standardized production of drug.
Summary of the invention
It is an object of the invention to solve the problems, such as that the anticonvulsion drug effect of existing Bombyx Batryticatus extract is inconsistent, bombyx batryticatus water is provided and is mentioned
Object is preparing the application in anticonvulsant drug, has in mind from the rigid time of bombyx batryticatus, it is determined that the bombyx batryticatus of drug effect preferably, more stable is stiff
Change the time, the standardized production for Chinese medicine bombyx batryticatus provides foundation.
The purpose of the invention is achieved by the following technical solution:
Bombyx batryticatus water extract is preparing the application in anticonvulsant drug；
The bombyx batryticatus is the bombyx batryticatus to ossify 5 days or more；Described rigid 5 days or more are since silkworm is susceptible at the point of death
It calculates.
Consider from production and cost of manufacture, the bombyx batryticatus that the bombyx batryticatus preferably ossifys 5-9 days.
The bombyx batryticatus water extract is made by following steps:
Bombyx batryticatus (powder) is weighed, water heating extracting is added several times with 1:5-1:10 solid-liquid ratio, each 1h or more, extracting solution filtering
After be concentrated, freeze-dried acquisition bombyx batryticatus water extract；
The preferred 1:5 of the solid-liquid ratio；
The heating extraction, is preferably heated to 80-100 DEG C；
The extraction time preferred 1h.
The present invention has the following advantages and effects with respect to the prior art:
The present invention has found an important factor for influence bombyx batryticatus water extract anticonvulsant efficacy accurately --- and ossify the time, provides drug effect
Preferably, more stable bombyx batryticatus ossifys the time, and the standardized production for Chinese medicine bombyx batryticatus provides foundation.
Detailed description of the invention
Fig. 1 is the influence column that bombyx batryticatus water extract and alcohol extract cause epilepsy death prevention rate rate to strychnine in embodiment 1
Figure；Wherein, S: model group, W1: water extract low dose group, W2: water extract middle dose group, W3: water extract high dose group, E1: alcohol
Extract low dose group, E2: alcohol extract middle dose group, E3: alcohol extract high dose group.
Fig. 2 is different rigid its cosmetic variation of the bombyx batryticatus of time.
Fig. 3 is that the bombyx batryticatus water extract of different rigid times in embodiment 2 causes the shadow of epilepsy death prevention rate rate to strychnine
Ring histogram；Wherein, S: model group, SW: normal silkworm, D1: ossify 1d bombyx batryticatus, D5:
Ossify 5d bombyx batryticatus, D9: ossify 9d bombyx batryticatus.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment 1
The anticonvulsant efficacy of bombyx batryticatus water extract and alcohol extract compares:
The effective substance that epilepsy mouse is caused to understand bombyx batryticatus to strychnine, by zoopery to bombyx batryticatus water extract and alcohol
The anticonvulsant action of extract is determined.
The preparation of bombyx batryticatus water extract: weighing bombyx batryticatus powder, and solid-liquid ratio 1:5-1:10 adds water to be heated to 100 DEG C of extractions 2 times, often
Secondary 1h is concentrated in vacuo, freeze-dried acquisition bombyx batryticatus water extract after extracting solution filtering.
The preparation of bombyx batryticatus alcohol extract: weighing bombyx batryticatus powder 150g, with ethanol solution refluxing extraction 2 times of 65%, solid-liquid ratio 1:
5-1:10, each 1h, extracting solution vacuum concentration, freeze-dried acquisition bombyx batryticatus alcohol extract.
Experimental method:
1, the method for breeding of experimental animal
Experimental animal is SPF grades of kunming mices of male, 18~22g.Rear adaptable fed 3d is bought, it is then that mouse is random
Grouping, every group 10.The pertinent regulations of management of laboratory animal and protection are abided by the raising of animal in experimentation.
2, modeling and medication
Water extract and alcohol extract are dissolved with sterile water.According to preset dose gastric infusion, model group is given isometric
Sterile water, continuous gavage 5d.After 30min is administered in 6d, the injection for curing of 1.6mg/kg taxi, continuous observation is injected intraperitoneally
30min, record clonic spasm incubation period (I-III grades of duration of seizure) of mouse, tetanic convulsion incubation period (V grades of breaking-outs) and
The number of elements of dead mouse in 30min.Pass through video monitoring mouse epilepsy outbreak process.
The observation of mice convulsion reaction is referring to Racine standard: 0 grade: reactionless；I grades: rhythmicity bicker, ear or facial flesh
Meat twitches clonic spasm；II grades: nodding and twitch clonic spasm with more serious facial muscles；III level: there is forelimb clonic spasm but be not accompanied by
Uprightly；IV grades: forelimb clonic spasm is with upright；V grades: generalized tonic-clonic breaks out and falls.
3, animal experimental data counts
Data carry out statistical analysis using 18.0 software of SPSS, and measurement data is with mean ± standard errorTable
Show, the comparison of multisample mean uses One-Way ANOVA variance analysis, and comparison among groups use LSD method.
Experiment sets the low, middle and high dose groups of model group, water extract and alcohol extract, and (dosage is respectively in terms of crude drug
10g/kg, 20g/kg and 30g/kg), water extract and alcohol extract are accordingly converted when dissolving according to its yield.Experimental result is shown in
Table 1 and Fig. 1:
1 bombyx batryticatus water extract of table and alcohol extract cause the influence of epilepsy mouse to strychnine
Compared with model group, p < 0.01 * p < 0.05, * *.
As can be seen from Table 1, bombyx batryticatus water extract high dose group being capable of extremely significant extension mouse clonic spasm incubation period (p < 0.01)
With tetanic incubation period (p < 0.01).Bombyx batryticatus water extract middle dose group also can significantly extend mouse tetanic incubation period (p < 0.05).
As seen from Figure 1, bombyx batryticatus water extract high dose group can also significantly improve the Death prevention rate of mouse.With model
Group is compared, and alcohol extract causes the improvement of epilepsy mouse not significant strychnine.
The above results show that bombyx batryticatus water extract causes the therapeutic effect of epilepsy mouse preferable strychnine, and apparent agent is presented
Graded effect is the anticonvulsant main pharmacodynamics material base of bombyx batryticatus.
Embodiment 2
Ossify influence of the degree to the anticonvulsion drug effect of bombyx batryticatus water extract:
Water extract preparation: weighing bombyx batryticatus powder, and solid-liquid ratio 1:5-1:10 adds water to be heated to 100 DEG C to extract 2 times, each 1h with
On, it is concentrated in vacuo after extracting solution filtering, freeze-dried acquisition bombyx batryticatus water extract, yield 25.21%.
Body surface shows typical bombyx batryticatus feature covered with white conidia powder after bombyx batryticatus ossifys 5 days.With bombyx batryticatus water extract into
The drug effect of the different rigid degree bombyx batryticatuss of row compares.Bombyx batryticatus progress hot water extraction to normal silkworm and ossify 1,5,9d, in terms of crude drug
The dosage of each sample is 25g/kg.As a result as shown in Table 2 and Fig. 3:
The bombyx batryticatus of the different rigid times of table 2 causes the influence of epilepsy mouse to strychnine
Compared with model group, *p< 0.05, * *p<0.01
The result shows that the drug effect of bombyx batryticatus is obvious related (table 2) to rigid degree.Compared with model group, the bombyx batryticatus for the 5d that ossifys
Also the clonic spasm incubation period (P < 0.05) of mouse can significantly be extended, the 9d bombyx batryticatus that ossifys can the extremely significant clonic spasm incubation period for extending mouse
(P < 0.01), the significant tetanic incubation period (P < 0.05) for extending mouse, moreover it is possible to significantly improve the Death prevention rate (figure of mouse
3).Each bombyx batryticatus sample drug effect is successively are as follows: D9 > D5 > D1 > SW shows that the rigid time is longer, and the degree that ossifys is higher, the medicine of bombyx batryticatus
It is better with quality.
To sum up, it is proposed that bombyx batryticatus ossifys the time as 5-9d, and the preparation of anticonvulsant drug is proposed for water.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (7)
1. bombyx batryticatus water extract is preparing the application in anticonvulsant drug.
2. application according to claim 1, it is characterised in that: the bombyx batryticatus is the bombyx batryticatus to ossify 5 days or more.
3. application according to claim 1, it is characterised in that: the bombyx batryticatus is the bombyx batryticatus to ossify 5-9 days.
4. application according to claim 1, it is characterised in that: the bombyx batryticatus water extract is made by following steps:
Bombyx batryticatus is weighed, water heating extracting is added several times with 1:5-1:10 solid-liquid ratio, each 1h or more is concentrated after extracting solution filtering,
Freeze-dried acquisition bombyx batryticatus water extract.
5. application according to claim 4, it is characterised in that: the solid-liquid ratio is 1:5.
6. application according to claim 4, it is characterised in that: the heating extraction is heated to 80-100 DEG C.
7. application according to claim 4, it is characterised in that: the extraction time is 1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811368712.3A CN109288870A (en) | 2018-11-16 | 2018-11-16 | Bombyx batryticatus water extract is preparing the application in anticonvulsant drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811368712.3A CN109288870A (en) | 2018-11-16 | 2018-11-16 | Bombyx batryticatus water extract is preparing the application in anticonvulsant drug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109288870A true CN109288870A (en) | 2019-02-01 |
Family
ID=65143678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811368712.3A Pending CN109288870A (en) | 2018-11-16 | 2018-11-16 | Bombyx batryticatus water extract is preparing the application in anticonvulsant drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109288870A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111437292A (en) * | 2020-06-08 | 2020-07-24 | 西南大学 | Bombyx Batryticatus water extract and application thereof |
| CN111494426A (en) * | 2020-06-08 | 2020-08-07 | 西南大学 | Bombyx Batryticatus alcohol extract and application thereof |
-
2018
- 2018-11-16 CN CN201811368712.3A patent/CN109288870A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 颜辉: "《僵蚕成分及药理作用研究进展》", 《中国蚕业》 * |
| 黄晓雪: "《僵蚕的生药学及药理活性研究》", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111437292A (en) * | 2020-06-08 | 2020-07-24 | 西南大学 | Bombyx Batryticatus water extract and application thereof |
| CN111494426A (en) * | 2020-06-08 | 2020-08-07 | 西南大学 | Bombyx Batryticatus alcohol extract and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1210047C (en) | Medicine for treating common cold and preparation method | |
| CN104547826A (en) | Medical application of galangin extract to treat dysmenorrhea | |
| CN109288870A (en) | Bombyx batryticatus water extract is preparing the application in anticonvulsant drug | |
| CN101926865B (en) | Spina date seed depression-resolving and nerve-soothing composition and preparation method thereof | |
| CN103417846B (en) | Chinese medicine composition of a kind of blood sugar lowering and preparation method thereof | |
| CN101843659A (en) | Traditional Chinese medicine granular formulation for preventing and treating influenza and preparation method thereof | |
| CN1397560A (en) | Process for extracting general platycodoside and platycoligenin D from platycodon root, its medical application and its Chinese medicine | |
| CN114470042B (en) | Composition for preventing and treating liver injury of animals as well as preparation method and application thereof | |
| CN1853681A (en) | Medicine for treating nervous prostration and improving sleep | |
| CN105395600A (en) | Application of cyclocarya paliurus extract in preparing medicine for treating cardiovascular and cerebrovascular diseases | |
| CN101474258B (en) | Ficus microcarpa var.pusillifolia extract, extracting method and application of the extract | |
| CN1857605A (en) | Medicine for treating chronic alcoholic hepatopathy and its preparing method | |
| CN1139553A (en) | Application of medicinal combination containing termite population and main nest bacterial nursery | |
| CN103768454B (en) | A kind of composition with prevention and treatment HIV/AIDS and preparation method thereof | |
| CN102631492B (en) | Chinese medicinal composition for improving immunity and resisting fatigue | |
| CN101366731B (en) | Propolis flavone, preparation method and uses in treating diabetes | |
| CN1237983C (en) | Compound rhizome of Cyrtomium aspirin tablet | |
| CN114903933B (en) | Traditional Chinese medicine for treating eperythrozoonosis of sheep as well as preparation method and application thereof | |
| CN102631486B (en) | Health care composition | |
| CN109758493B (en) | Application of cardamine hirsute and extract thereof in preparation of drugs for preventing or treating arrhythmia | |
| CN102631506B (en) | Traditional Chinese medicine composition capable of improving immunity and resisting fatigue | |
| CN102631487B (en) | Traditional Chinese medicine composition capable of improving immunity and resisting fatigue | |
| CN117064962B (en) | Tung or extract thereof, composition thereof and use thereof | |
| CN102631393B (en) | Health care composition | |
| CN106727799A (en) | A kind of health-preserving health-care Chinese medicine composition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190201 |