CN1139553A - Application of medicinal combination containing termite population and main nest bacterial nursery - Google Patents
Application of medicinal combination containing termite population and main nest bacterial nursery Download PDFInfo
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Abstract
In the medicinal composite, the weight ratio of termite to the termitophile is 1:15-20. Said medicinal composite can be used to treat the hepatitis of mammal including mankind for anti-inflammation and analgesia, to cure arthritis, and sterility. It possesses of also the action of relieving a cough and enhancing the cold-resistance capability.
Description
The present invention relates to a kind of pharmaceutical composition and use, particularly relate to a kind of application that comprises the pharmaceutical composition of termite population and main nest bacterial nursery thereof.
As everyone knows, Coptotermes formosanus Shtrari. is one of five universally acknowledged major pests, harm forest, dyke, earth dam, even house.Kind surplus the known Coptotermes formosanus Shtrari. in the whole world reaches 2000, kind surplus China has 360 approximately is distributed in 25 provinces (district), municipality directly under the Central Government on the south the Dandong.Being distributed widely in kind of forest surplus Odontotermes formosanus Shiraki [Odontotermes formosanus (shiraki)] the harm Lignum seu Ramulus Cunninghamiae Lanceolatae etc. 70 of all provinces in China south, is the primary pest of agricultural and water conservancy.Show that according to investigations the Odontotermes formosanus Shiraki nest can reach more than 3000 in thousand mu of lanceolata forest, population quantity is extremely abundant.People know that already Coptotermes formosanus Shtrari. itself can be edible, the chicken that fungus garden grows from termitarium
The delicious especially dish of bacterium, some are edible with it per capita in the home and abroad.
Coptotermes formosanus Shtrari. is under the jurisdiction of the Insecta Isoptera, with other insecticides, particularly the same with the Formica fusca and the Apis that belong to the world three great society insecticides together, 18 seed amino acids and the various trace elements that comprises selenium, zinc that contain needed by human body, it is the nutrition treasure-house of a seat minitype, be used for health care and cure the disease, big profit is arranged and do not have little fraud.Simultaneously, the Coptotermes formosanus Shtrari. campsite is life down, lives in caves moist ecological environment and huge life and fertility are arranged; Even Coptotermes formosanus Shtrari. death, mildew is not rotten yet in nest; Coptotermes formosanus Shtrari. is got the basic nutrition material (juice) of the various trees of food; With the symbiotic chicken of Odontotermes formosanus Shiraki
Bacterium, existing edibility has drug effect (contain total flavones, sedative action is arranged) again.
The medical value of relevant Coptotermes formosanus Shtrari. and fungus garden thereof, oneself reports in Chinese medicine to some extent.
Li Shizhen (1518-1593 A.D.) is put down in writing Coptotermes formosanus Shtrari. is existing in Compendium of Material Medica.To the ant ovum, Li Shizhen (1518-1593 A.D.) is said " its Luan Ming Ant-eggs.Recluse dig it, have to bucket stone person.The stone people eats it, so the bean of " then interior ", " all officials " feedback food has fish or meat paste also ".Qing Dynasty's ZHAO Xue-Min is said in supplementary Amplifications of the Compendium of Materia Medica and Formica fusca and ant ovum " sweet flat, the little poison of nature and flavor, physical strength profiting, damp color (beauty treatment), lactogenic juice ".Be used for being short of physical strength after being ill, puerperal hypogalactia etc., the 9-15g parching to brown, the face of grinding is taken after mixing it with water.
The high-quality fungus garden of Coptotermes formosanus Shtrari., Li Shizhen (1518-1593 A.D.) " Coptotermes formosanus Shtrari. mud (being ant nest) cures mainly the malignant boil toxic swelling, and the last person of Zhou Songmu is black with each stir-fry of Plumbum preparatium, grinds with fragrant stain and is coated with it, and getting more is only " also early on the books in Compendium of Material Medica.With the symbiotic chicken of fungus garden of Odontotermes formosanus
Bacterium, but hyoscine.Li Shizhen (1518-1593 A.D.) is pointed out in Compendium of Material Medica, chicken
" abnormal smells from the patient is sweet, flat, nontoxic for bacterium.Cure mainly stomach reinforcing, clear refreshing, control hemorrhoid "." middle traditional Chinese medicines sea " pointed out also that its property is hidden and returned through " sweet, flat.Nontoxic.Go into the heart, lung, spleen three warps, it is refreshing clearly to cure mainly invigorating the spleen and regulating the stomach, QI invigorating.
As seen by above-mentioned, termitarium just is widely used in the world of medicine since ancient times, has magical effects such as physical strength profiting, tonify deficiency spleen invigorating.
In recent years, Coptotermes formosanus Shtrari. has been had the report of some application.CN1090760A proposes the pure Coptotermes formosanus Shtrari. of a kind of usefulness as raw material, to soak the extract that technologies such as extraction obtain, and mixes the health care medicine of making after stirring with Fructus Crataegi powder, and this medicine has the effect that improves the human body body's immunity, and some tumor is had therapeutic effect.
The antitumor activity that the Coptotermes formosanus Shtrari. ethanol extract had the S180 tumor-bearing mice and the research of immunoregulation effect thereof in " modern Application medical science " the 10th volume the 5th phase (1993) 29-30 page or leaf, have been reported.
CN1098628A provides with Coptotermes formosanus Shtrari. and fungus garden thereof and has been raw material, adopts water extraction and alcohol extraction to obtain extract, extract is made pelletize behind oral liquid or the concentrate drying, the product that obtains has that nourishing is refreshed oneself, spleen invigorating hepatoprotective, effect of prolonging life, and point out that it can improve the immunocompetence of body, hypermnesis and alert and resourceful power etc.
Though in the prior art the medical value to Coptotermes formosanus Shtrari. and fungus garden thereof be described and utilized, Coptotermes formosanus Shtrari. and its fungus garden also are far from checking on for the effect of treatment disease.
In addition, because Coptotermes formosanus Shtrari. is harmful to forest, for a long time, people are devoted to kill Coptotermes formosanus Shtrari. always, and not lure kill Coptotermes formosanus Shtrari., destroy termitarium in, Coptotermes formosanus Shtrari. and ant nest resource are effectively utilized.
The objective of the invention is to effectively utilize Coptotermes formosanus Shtrari. and fungus garden thereof, change harmful to treasure, the medical treatment and the health purpose of the pharmaceutical composition that contains Coptotermes formosanus Shtrari. and fungus garden medicinal component thereof is provided.
For finishing above-mentioned purpose, the pharmaceutical composition that includes the dry body of the termite nest fungus comb that contains termite population provided by the invention comprises human chronic viral hepatitis preparation treatment mammal, avoid hepatic injury, antiinflammatory, detumescence, treatment of arthritis, and the application in the medicine of cough-relieving, and improving application in the health medicine that mammal comprises the human body tolerance to cold in preparation, the pharmaceutical composition that the present invention further provides the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human infertility preparation treatment mammal.
In the pharmaceutical composition of the described dry body that includes the termite nest fungus comb that contains termite population, the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described medicine: 15-20.
Described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)) preferably, and described termite nest fungus comb is a chicken
Bacterium (Termitomyces albuminosus (Berk) Heim).
Described pharmaceutical composition can contain or not contain other medicinal or non-medicinal ingredients, as containing other Chinese medicine ingredients.
The above-mentioned pharmaceutical composition that includes the dry body of the termite nest fungus comb that contains termite population prepares set by step:
1) cuts off the ant road that Coptotermes formosanus Shtrari. escapes, make and contain Coptotermes formosanus Shtrari. in the fungus garden, preferably make Coptotermes formosanus Shtrari. colony major part all in fungus garden;
2) fungus garden is dug out;
3) with the above-mentioned termite nest fungus comb dewatered drying that contains termite population that digs out, as oven dry under about 80 ℃, pulverizing, decontaminations then;
4) sterilization.
Because the composition more complicated of Coptotermes formosanus Shtrari. and fungus garden thereof, and itself be eaten and there is no how many harms,, avoided losing of effective ingredient, and make the easier enforcement of method of the present invention so the present invention has adopted directly the method for Coptotermes formosanus Shtrari. and its fungus garden dried.
The present invention further comprises by the pharmaceutical composition of method for preparing and the preparation that contains described pharmaceutical composition, as tablet, capsule etc.
By the present invention, effectively utilized the Coptotermes formosanus Shtrari. resource, particularly endanger Odontotermes formosanus Shiraki and bacterium nest thereof in China Pinus massoniana Lamb, lanceolata forest, rubber woods or the Eucalyptus woods, change harmful to treasure.
Simultaneously, the invention provides new medicinal raw material.Can prevent that people from utilizing the Formica fusca resource useful to plant growing too much, uses as medicinal raw material as big Black Hills Formica fusca, thereby reach the effect that protects beneficial insects.
Below describe the present invention in detail.
The present invention selects the termite nest fungus comb that contains termite population for use, the termite nest fungus comb that particularly contains Odontotermes formosanus Shiraki, it is widely distributed in China the greater part, as Liaoning, Guangdong, Guangxi, Hunan, Hubei, Shandong, Shanxi, Jiangsu, Zhejiang, Jiangxi, Taiwan, ground such as Fujian, Anhui, Sichuan, Yunnan, Shaanxi, Tibet, Hebei, Henan, abroad, as Burma, Thailand, Vietnam etc. distribution is arranged also.For the quality that guarantees drug prepared based on winter, spring (between October-April next year), at first be to select Odontotermes formosanus Shiraki work nest, its main nest degree of depth is about 0.5-1 rice in China south about 1.5-3.0m.Cut off the ant road that Coptotermes formosanus Shtrari. escapes, make Coptotermes formosanus Shtrari. colony major part all in fungus garden, after digging out, as early as possible fungus garden is taken back indoor; Selecting main nest is that filemot fungus garden (including termite population) is used constant temperature oven, preferably about 80 ℃, with the fungus garden dewatered drying; Then, the fungus garden of Odontotermes formosanus after will drying with pulverizer is pulverized, and sieves, and as crossing 80 mesh sieves, collects dry powder; Dry powder as 100 ℃ of constant temperature sterilizations 60 minutes, through method known to a person of ordinary skill in the art, is made the pharmaceutical composition that includes the dry body of the termite nest fungus comb that contains termite population of the present invention through sterilization.
The medical active that the present invention comprises the pharmaceutical composition of termite population and main nest bacterial nursery thereof is illustrated by following.
Studies show that Coptotermes formosanus Shtrari. and bacterium nest thereof contain 18 seed amino acids of needed by human body and comprise the various trace elements and the vitamin E of selenium, zinc, wherein Coptotermes formosanus Shtrari. contains higher protein, formic acid, cursive script formaldehyde and cholesterol like epinephrine 17-hydroxy-11-dehydrocorticosterone sample material.Chicken
Bacterium then contains a large amount of total flavones (flavone has sedative action).A large amount of zooperies prove that Coptotermes formosanus Shtrari. and bacterium nest thereof have tangible antiinflammatory, detumescence, analgesic activity, and the reduction glutamate pyruvate transaminase is arranged, and the liver protecting is avoided the damage of poisonous substance, and promote the repair function behind the hepatocyte acute injury.Clinically be used for human experimentation and also show, it is all effective in cure to rheumatoid and rheumatic arthritis, senile osteoporosis, insomnia, constipation and other diseases to take pharmaceutical composition of the present invention, and has effects such as the defying age of being good for the stomach and enhance immunity effect.
The traditional Chinese medical science is attached great importance to " healthy energy " of human body.Human righteousness's deficiency just can not be resisted the generation of disease or the infringement of elimination pathogenic factor effectively; And healthy energy plentifully just can overcome the harm of pathogenic factor in the disease progression process and be easy to rehabilitation.As seen, whether vigorous healthy energy is, with the generation of disease, development and after more confidential relation is arranged all.Therefore, all genus have a delicate constitution, positive QI-insufficiency, all need utilization to transfer and mend health invigorating and resistance against diseases.
The different causes of disease acts on different body constitution and forms deficiency syndrome, its pathogenesis character and change of disease trend, though complicated unusually, various, its pathogenesis that adds up to changes not outer QI and blood negative and positive weakness and cardiopulmonary taste Liver and kidney void is lost.Its middle kidney and spleen are very important on the pathogenesis that causes void.Taste are the vital essence the source of vital function, if weakness of the spleen and stomach, and indigestion then, whole body loses supports, and deficient depletion is all extremely.Famous traditional Chinese medical science works " Principle and Prohibition for Medical profession " is said: " then blood is living for diet, and blood is not given birth to the then inferior sun of joining of deficiency of YIN, and the five internal organs that are bound to decrease together ".
For the treatment of deficiency syndrome, should be with " deficiency syndrome should be treated by tonifying method ", " damage person's benefit ", " diseases caused by over-strain should be treated by warming " and " weakened configuration person, warming with drugs of thick nature; The insufficiency of essence person, tonifying with drugs of thick flavor " be fundamentum.But it is congenital that the viscera QI and blood derives from, and grow provisions the day after tomorrow, so transfer the key point of spleen reinforcing kidney for the treatment deficiency syndrome.
The pharmaceutical composition that comprises termite population and main nest bacterial nursery thereof of the present invention is highly suitable for weak treatment of conditions and health cares such as deficient syndrome insomnia.Practical experience also shows that this product sets upright tonify deficiency, and is safe and effective.
Hepatitis is because due to the hepatites virus infections, and the traditional Chinese medical science thinks that damp-heat virus invades human body, and heresy just must hindered, and causes the human body deficiency of both YIN and YANG, and damp-heat accumulation is in liver, QI stagnation of the liver and galibladder then, and the liver belonging to wood, kidney belongs to water, and liver is the son of kidney in the five elements, the sick cover of son mother-qi, and be impairment of the kidney.The liver and the kidney have a common source, and the kidney being the origin of congenital constitution, thereby show as susceptible to lose temper due to restlessness, and dry mouth and tougue is eaten not abdominal distention, dull pain in liver etc.With the passing of time the stagnation of QI must become the deficiency of vital energy, shows as weak, the Mental fatigue of the deficiency of vital energy.Pharmaceutical composition of the present invention has the merit of tangible health invigorating, strengthening vital QI to eliminate pathogenic factors.Positive QI-insufficiency, the generation of resist the disease is effectively eliminated pathogenic factor the harm of human body is easy to rehabilitation.The modern medicine discovery, after hepatitis virus is invaded human body, massive duplication in liver, and constantly destroy hepatocyte, the clinical glutamate pyruvate transaminase that mainly shows as sharply raises.A large amount of zooperies and clinical trial confirm that pharmaceutical composition of the present invention has the effect of obvious reduction glutamate pyruvate transaminase, can avoid poisonous substance (CCl by the liver protecting
4) damage, and the difficult repair function that promotes behind the hepatocyte acute injury.
Rheumatoid arthritis belongs to motherland's medical science " arthromyodynia ", " severe and migratory arthralgia " category, the traditional Chinese medical science think " numbness person closes also, with the QI and blood be must not close of heresy pass through and the pain also ".The generation of arthromyodynia is except that experiencing the rheumatism heresy, and the power of health plays an important role." Qi Shengfang " spells out, " all because of body void, empty in the natural fibre line of meat, the dampness of having a cold forms numbness also ".Since the deficiency of vital energy, YANG QI deficiency, Wei Yang loses solid, and wind, cold, wet three heresies are taken advantage of a weak point, and the arteries and veins road that flows away causes the QI and blood retardance, and therefore, " strengthening vital QI to eliminate pathogenic factors " is the basic rule of treatment of treatment arthromyodynia.
Pharmaceutical composition of the present invention is stated in the remarkable effect front aspect " strengthening vital QI to eliminate pathogenic factors ".Pharmacological evaluation confirms that also it has obvious effect at antiinflammatory, detumescence, ease pain, all has definite curative effect to rheumatoid arthritis, senile osteoporosis, parosteitis etc.
Aspect reproduction, the pharmaceutical composition that comprises termite population and main nest bacterial nursery thereof of the present invention can make because of old and feeble or sterilely cause testis and ovary weight to descend increasing to normal level, the interior DNA of Interstitial cell and spermatocyte, rna content increase in the convoluted seminiferous tubule, promote cell division, make infertile person's secretory function obtain adjusting, improved hypothalamic-pituitary-ovarian (testis) axle function.Make that deficiency of kidney-QI is weak, kidney not store essential substances, activating QI, give birth to marrow and functional rehabilitation, treat infertility effectively.
The pharmaceutical composition that includes the dry body of the termite nest fungus comb that contains termite population of the present invention has antitussive action.
Pharmaceutical composition of the present invention can be made various multi-form peroral dosage forms by method known to a person of ordinary skill in the art, makes capsule as directly incapsulating, and is pressed into tablet after filler mixes, or makes suspension etc.
The pharmaceutical composition that comprises termite population and main nest bacterial nursery thereof of the present invention is used for the treatment of antiinflammatory, detumescence, ease pain to be used, and consumption is 0.5-3g/d; Consumption is 0.5-3g/d when being used for the liver protecting, the reduction paddy third ammonia enzyme.When using as cough suppressing medicine, consumption is 0.25-3g/d; When being used for the treatment of the infertility use, consumption is 0.5-3g/d; When using as the health care medicine, consumption is 0.25-1g/d.
Animal experiment shows that the pharmaceutical composition that the present invention comprises termite population and main nest bacterial nursery thereof is safe in the use.Limited in the acute toxicity testing because of the mice gastric capacity, irritate to feed with 250mg/ml and can't try to achieve oral LD
50, its maximum tolerated dose is 37.5g/kg, is equivalent to 200-900 times of clinical consumption.Long term toxicity test adopts health care dosage 200 times of (4g/kg), 400 times (8g/kg), the therapeutic dose that are higher than clinical practice to carry out 12 all toxicity for 50 times and 100 times and observes, its result shows, take pharmaceutical composition of the present invention for a long time and do not damage the health status of animal, do not influence hematological every index, do not influence the function of internal organs such as liver, kidney.As seen pharmaceutical composition of the present invention can uninterruptedly be taken for a long time, and is safe and reliable.
Nearly hundred routine volunteers take pharmaceutical composition of the present invention March-5 year, have verified following zooperal drug effect, and have found no any toxic and side effects.
Describe the present invention by the following examples in detail, but do not limit the present invention.
Embodiment 1
In the lanceolata forest in Jurong, Jiangsu Province county, select main nest at the Odontotermes formosanus Shiraki of the 1.5-3.0m nest of living, cut off the ant road that Coptotermes formosanus Shtrari. escapes, make Coptotermes formosanus Shtrari. colony major part all in fungus garden, after digging out, as early as possible fungus garden is taken back indoor; Selecting main nest is that filemot fungus garden (including termite population) fixes on about 80 ℃ with calorstat, temperature, with the fungus garden dewatered drying; Fungus garden after will drying with pulverizer is pulverized, and crosses 80 mesh sieves and becomes powder, obtains dry powder 1000 grams; Dry powder is 100 ℃ of constant temperature sterilization 60 minutes, and under aseptic condition, wherein 500 grams are preserved in packing is placed on the environment of cold drying, and other 500 grams incapsulate every 0.25g.
Embodiment 2
In the masson pine forest in suburb, Nanning, select main nest at the Odontotermes formosanus Shiraki of 0.5-1.0m nest alive, repeat the method for embodiment 1, the product made 500 grams are preserved in packing is placed on the environment of cold drying, and other 5.00 grams add filleies and are pressed into every tablet of tablet that contains present embodiment product 0.25g.
Embodiment 3
In the geographic rubber woods in Xishuangbanna, Yunnan, select main nest at the Odontotermes formosanus Shiraki of 0.5-1.0m nest alive, repeat the method for embodiment 1, the product made 500 grams are preserved in packing is placed on the environment of cold drying, and other 500 grams add filleies and are pressed into every tablet of tablet that contains present embodiment product 0.25g.
Embodiment 4
In the Eucalyptus woods in the Lezhou Peninsula, Guangdong, select main nest at the Odontotermes formosanus Shiraki of the 0.5-1.0m nest of living, repeat the method for embodiment 1, product 500 grams of making are preserved in packing is placed on the environment of cold drying, other 500 grams incapsulate, and every contains present embodiment product 0.25g.
Following examples are used to the effect that illustrates that pharmaceutical composition of the present invention has.Embodiment 5-8 illustrates its anti-inflammatory and analgesic effect; Experimental example 9 shows its liver protection effect; Embodiment 10 shows that it improves the effect of tolerance to cold; Embodiment 11 its therapeutical effect of explanation to infertility; The antitussive action of embodiment 12 explanations pharmaceutical composition of the present invention.Wherein, described pharmaceutical composition is meant the pharmaceutical composition that comprises termite population and main nest bacterial nursery thereof.
Among the following embodiment, used experimental animal all derives from Chinese Academy of Medical Sciences's Experimental Animal Center, and medicine is the commercially available prod.
5 pairs of arthritic influences of rat formaldehyde of embodiment
1, medicine preparation:, add 0.5% methylcellulose and fully grind and be made into suspension to put 4 ℃ of refrigerators standby with the dry powder of embodiment 1 gained.
2, material and method: getting body weight is 40 of the Wistar rats (♂ ♀ half and half) of 160-180 gram, divide four groups, be matched group and three dosage groups, 1.6 gram/kg/day, 3.2 gram/kg/day, 6.4 gram/kg/day, after stomach was irritated in experiment in first day one hour, cause inflammation with 2.5% formalin 0.06ml in Mus left and right sides limb foot sole of the foot middle part subcutaneous injection, survey left and right sides joints of foot girth after 5 hours.2-5 days every days medicine feed once, 1 hour repetition measurement joints of foot behind the medicine is obtained the poor of left and right sides joints of foot girth, represents swelling degree (being the inflammation degree) with this.
3, result: see Table table one dosage animal inflammation degree (X ± SD) (mm)
Number
(only) the 5th hour the 2nd day the 3rd day the 4th day the 5th day 0.5% first fine 10 2.60 ± 0.52 2.70 ± 0.48 2.25 ± 0.42 1.55 ± 0.50 1.85 ± 0.58
★★???????★★1.6g/kg/d??8??2.25±0.89?2.13±0.79?1.81±0.59?1.38±0.44?1，63±0.44
★3.2g/kg/d?10?2.35±0.58?2.00±0.62?1.60±0.66?1.05±0.37?0.95±0.16
△??????★???△???★★??△△??★★?△△6.4g/kg/d?12?2.42±0.70?1.58±0.76?0.92±0.63?0.42±0.51?0.17±0.33
★ ★ △ △ ★ ★ △ △ ★ ★ △ △ ★ ★ △ △ self is relatively: ★ P＜0.05 ★ ★ P＜0.01 with compare: △ P＜0.05 △ △ P＜0.01
4, conclusion: 6.4g/kg/d and 3.2g/kg/d group PARA FORMALDEHYDE PRILLS(91,95) arthritis have tangible resist inflammation on repercussive function.
The influence of inflammation due to 6 pairs of rat ears of embodiment dimethylbenzene
The medicine preparation is with embodiment 5.
1, material and method: 40 of Wistar rats, body weight are 160-180g (♂ ♀ half and half), are divided into 4 groups, and medicine 6.4g/kg/d, 3.2g/kg/d, the 1.6g/kg/d with above-mentioned preparation irritates stomach respectively, once a day, and continuous 6 times.Matched group is fed isopyknic 0.5% methylcellulose.After the last administration, caused inflammation at rat left side ear melted paraxylene 0.1ml in 1.5 hours, get the same position of rat two ears ear disk with the card punch of diameter 9mm after 15 minutes and weigh, represent the inflammation degree with the difference of two auricles.
2, result: see Table two tables two
| Drug dose | Number of animals (only) | Two auricle differences (X ± SD) (mg) | The P value |
| 0.5% first fibre | ????10 | ????102.70±9.80 | |
| ?1.6g/kg/d | ????8 | ????100.13±9.05 | |
| ?3.2g/kg/d | ????10 | ????90.60±12.06 | ＜0.05 |
| ?6.4g/kg/d | ????12 | ????63.67±7.15 | ＜0.01 |
3, conclusion: 6.4g/kg/d and 3.2g/kg/d group has tangible resist inflammation on repercussive function to inflammation due to the rat ear dimethylbenzene.
The influence of inflammation due to 7 pairs of mice ears of embodiment dimethylbenzene
The medicine preparation is with embodiment 5.
1, material and method: 30 of kunming mices, body weight 18-22g, ♂ ♀ half and half divides 3 groups, and medicine 3.2/kg/d and the 1.6g/kg/d with above-mentioned preparation irritates stomach respectively, once a day, continuous 5 times.Matched group is fed isopyknic 0.5% methylcellulose.After the last administration, caused inflammation at rat left side ear melted paraxylene 0.05m1 in 1.5 hours, get the same position of mice two ears ear disk with the card punch of diameter 9mm after 15 minutes and weigh, represent the inflammation degree with the difference of two auricles.
2, result: see Table three tables three
| Drug dose | Number of animals (only) | Two auricle differences (X ± SD) (mg) | The P value |
| 0.5% first fibre | ????10 | ????35.15±6.88 | |
| ?1.6g/kg/d | ????10 | ????32.89±7.82 | |
| ?3.2g/kg/d | ????10 | ????25.67±7.85 | ＜0.05 |
3, the pharmaceutical composition of the present invention of conclusion: 3.2g/kg/d is irritated stomach has tangible resist inflammation on repercussive function to inflammation due to the mice ear dimethylbenzene.
The influence of 8 pairs of mice acetic acid of embodiment peritonitis writhing response
The medicine preparation is with embodiment 5.
1, materials and methods: 40 of Kunming mouses, body weight 18-22g divides 4 groups at random, and 10 every group, male and female half and half.Every day medicine feed once, be administered three times altogether.2 hours lumbar injection 0.6% acetic acid 0.2ml cause pain after administration for the third time, and record gives behind the chemical algogen white mice writhing response number of times in 15 minutes.And carry out statistical with matched group, the results are shown in Table four.Table four group dosage number of animals is on average turned round the body number of times
(product indometacin 10mg/kg * 3 10 * *, 3.4 ± 4.0*P＜0.05**P＜0.001 of product embodiments 1 12g/kg * 3 10 * * 4.0 ± 3.9 of X ± S.D distilled water 0 10 29.1 ± 13.1 embodiment 1 6g/kg * 3 10 * 14.1 ± 10.2 of g/kg * d)
2, conclusion: 2 dosage of pharmaceutical composition of the present invention all have significant analgesic activity.The analgesic effect of 12g/kg dosage is identical with indometacin.
Embodiment 9
The medicine preparation is with embodiment 5.
1, material method: 30 of kunming mices, ♂ ♀ dual-purpose is divided into 3 groups, administration group, the positive and negative control group.Administration group dosage is 5g/kg/d, continuous once a day six days.At the noon before that day of the administration first time and the subcutaneous injection CCl in afternoon of the 4th administration
4(40 μ l/ only).The same subcutaneous injection CCl of positive controls
4, but medicine feed not.Got blood on the 7th day in test, press reitman-frankel method and measure gpt activity, and do the liver section inspection.
2, result:
(1) the glutamate pyruvate transaminase measurement result sees Table five
Table five
| Drug dose | Number of animals (only) | GPT activity (X ± SD) (unit/100ml blood) | The P value |
| Positive control | ????10 | ????150.8±42.2 | |
| The medicine feed group | ????10 | ????52.9±24.3 | ＜0.01 |
| Negative control | ????10 | ????50.0±10.6 |
(2) liver section is observed: the animal livers lobule central area of positive control destroys serious, and the liver of the animal of taking medicine is repaired and similar with negative control gradually.
3, conclusion: the mensuration by sections observation and transaminase activity shows: pharmaceutical composition of the present invention has the effect that reduces gpt activity.Remarkable with the positive controls comparing difference, and near the level of negative control.There is the liver protecting to avoid poisonous substance (CCl
4) damage, and promote repair function behind the cell acute injury.
The influence that 10 pairs of mices of embodiment are cold-resistant
1, medicine preparation:
The dry powder of embodiment 2 ground, 200 mesh sieves, was made into the 200mg/ml suspension with 0.5% methylcellulose.
2, materials and methods:
Kunming mouse, male, body weight 18-22g totally 36, is divided into three groups: (1) matched group at random; (2) 4g/kg group; (3) 8g/kg group.Gavage the medicine of being prepared, once a day, totally 11 times.
Matched group and two experimental group animals are put into large beaker respectively, place-25 ℃ of cryogenic refrigerators, after freezing 2 hours, take out statistics and respectively organize death and surviving animals number.
3, result: see Table six
Table six
| Group | Number of animals (only) | -25 ℃ 2 hours | The P value | ||
| Survival number (only) | Death toll (only) | Mortality rate (%) | |||
| Matched group 4g/kg group 8g/kg group | ????12 ????12 ????12 | ????2 ????7 ????9 | ????10 ????5 ????3 | ????83.3 ????41.7 ????25 | ＜0.05 ＜0.01 |
4, conclusion: at the same terms, each treated animal survival number difference is handled by statistics, experimental group and matched group have shown difference, the 8g/kg group compares with matched group, and difference highly significant (P＜0.01) shows that pharmaceutical composition of the present invention truly has constitutional function.
The clinical trial of 11 pairs of treatments of embodiment infertility
The women of child-bearing age's 20 examples, primary infertility 15 examples, Secondary cases 5 examples.The main infertile cause of disease is a menoxenia, and basal body temperature (BBT) curve display is less than 12 days, luteal phase such as follicular development difference etc.Take the capsule of embodiment 1 after 90 days by 1g/d, menoxenia is improved, and the BBT curve is two-phase, and luteal phase was greater than 13 days, follicular development is to more than the 1.8cm, occur interstitialcellstimulating hormone (ICSH) (LH) peak during ovulation, it is good that the CM of local condition increases character, through 2-3 course of therapy (30 days per courses of treatment, every day 1g), doing well,improving is comparatively satisfied, produce effects person's 17 examples, and wherein 3 examples are conceived.
Man's 10 examples are primary sterility, oligospermatism 3 examples, and lack of quantity of spermatic fluid is in 1.0ml 5 examples; Low sperm activity 2 examples; By the back (1g/d, 90 days) of taking medicine, 10 routine patients have positive effect, and particularly the lack of quantity of spermatic fluid person can both reach greater than 2.0ml after March.
Embodiment 12 antitussive actions
Case 1: certain woman, 56 years old, sudden irritable cough, the annual lasting several months, and with lumbago and skelalgia, various treatments are all ineffective.Outbreak back is by every day 2 times, each 2 capsules of taking embodiment 1, cough in the 3rd day takes a turn for the better, obeyed 100 after, not seeing the cough recurrence over one year, and waist and leg pains has been cured also.
Case 2: certain woman, 39 years old, no expectorant dry cough, annual outbreak is taken Chinese medicine, various antiinflammatory Western medicine is all ineffective, and annual outbreak continues the several months.After non-quantitative was taken the capsule (1-2 grain/sky) of embodiment 1, a week took effect, and does not see recurrence after the drug withdrawal.
Claims (20)
1, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human chronic viral hepatitis preparation treatment mammal.
2, application as claimed in claim 1 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.
3, application as claimed in claim 1 or 2 is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (Shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
4, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human antiinflammatory, detumescence, treatment of arthritis the preparation mammal.
5, application as claimed in claim 4 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.
6, as claim 4 or 5 described application, it is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
7, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population improves application in the medicine that mammal comprises the human body tolerance to cold in preparation.
8, application as claimed in claim 7 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.
9, as claim 7 or 8 described application, it is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
10, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human infertility preparation treatment mammal.
11, application as claimed in claim 10 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.
12, as claim 10 or 11 described application, it is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
13, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human cough-relieving the preparation mammal.
14, application as claimed in claim 13 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.
15, as claim 13 or 14 described application, it is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
16, a kind of preparation includes the method for pharmaceutical composition of the dry body of the termite nest fungus comb that contains termite population, comprises following steps:
1) cuts off the ant road that Coptotermes formosanus Shtrari. escapes, make and contain Coptotermes formosanus Shtrari. in the fungus garden;
2) fungus garden is dug out;
3), pulverize then, decontamination with the above-mentioned termite nest fungus comb dewatered drying that contains termite population that digs out;
4) sterilization.
17, method as claimed in claim 16, it is characterized in that: Coptotermes formosanus Shtrari. colony major part is all in fungus garden in the described fungus garden of Odontotermes formosanus, and the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20, oven dry is carried out under 80 ℃.
18, method as claimed in claim 16 is characterized in that: described Coptotermes formosanus Shtrari. is Odontotermes formosanus Shiraki (Odontotermes formosanus (shiraki)), and described termite nest fungus comb is a chicken
Bacterium (Termito-myces albuminosus (Berk) Heim).
19, method as claimed in claim 16 is characterized in that: described termite population and termite nest fungus comb are taken from masson pine forest, lanceolata forest, the rubber woods, the woodsy Odontotermes formosanus Shiraki of eucalyptus globulus (Odontoter-mes formosanus (Shiraki)) nest.
20, contain pharmaceutical preparation just like the pharmaceutical composition of the method for one of claim 16-19 preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95107728A CN1067555C (en) | 1995-06-30 | 1995-06-30 | Application of medicinal combination containing termite population and main nest bacterial nursery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95107728A CN1067555C (en) | 1995-06-30 | 1995-06-30 | Application of medicinal combination containing termite population and main nest bacterial nursery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1139553A true CN1139553A (en) | 1997-01-08 |
| CN1067555C CN1067555C (en) | 2001-06-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95107728A Expired - Fee Related CN1067555C (en) | 1995-06-30 | 1995-06-30 | Application of medicinal combination containing termite population and main nest bacterial nursery |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1067555C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101200680B (en) * | 2007-10-25 | 2010-11-17 | 樊德江 | Application of termite nest fungus comb in wine |
| CN102138940A (en) * | 2011-03-24 | 2011-08-03 | 江苏苏南药业实业有限公司 | Preparation method of termitarium capsule |
| CN102293825A (en) * | 2011-08-29 | 2011-12-28 | 江苏苏南药业实业有限公司 | Compound termitarium and preparation method thereof |
| CN101757614B (en) * | 2010-02-22 | 2012-04-04 | 苏州汇涵医用科技发展有限公司 | Hemostat and preparation method thereof |
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| CN106109748A (en) * | 2016-06-29 | 2016-11-16 | 江苏苏南药业实业有限公司 | A kind of Chinese medicine composition containing termitarium and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1090760A (en) * | 1993-08-23 | 1994-08-17 | 武汉市白蚁防治所 | A kind of production method of health care medicine Coptotermes formosanus Shtrari. dry powder |
| CN1098628A (en) * | 1994-06-02 | 1995-02-15 | 陈远传 | Coptotermes formosanus Shtrari. and bacterium plot series preparations thereof |
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| US8221713B2 (en) | 2007-03-30 | 2012-07-17 | Tsinghua University | Method for making metal titanate |
| CN101200680B (en) * | 2007-10-25 | 2010-11-17 | 樊德江 | Application of termite nest fungus comb in wine |
| CN101757614B (en) * | 2010-02-22 | 2012-04-04 | 苏州汇涵医用科技发展有限公司 | Hemostat and preparation method thereof |
| CN102138940A (en) * | 2011-03-24 | 2011-08-03 | 江苏苏南药业实业有限公司 | Preparation method of termitarium capsule |
| CN102138940B (en) * | 2011-03-24 | 2012-07-04 | 江苏苏南药业实业有限公司 | Preparation method of termitarium capsule |
| CN102293825A (en) * | 2011-08-29 | 2011-12-28 | 江苏苏南药业实业有限公司 | Compound termitarium and preparation method thereof |
| CN102293825B (en) * | 2011-08-29 | 2013-05-29 | 江苏苏南药业实业有限公司 | Compound termitarium and preparation method thereof |
| CN106109748A (en) * | 2016-06-29 | 2016-11-16 | 江苏苏南药业实业有限公司 | A kind of Chinese medicine composition containing termitarium and preparation method thereof |
| CN107137488A (en) * | 2017-05-27 | 2017-09-08 | 江苏苏南药业实业有限公司 | A kind of compound termitarium particle of anti-osteoarthritis |
| CN107410931A (en) * | 2017-08-11 | 2017-12-01 | 句容市森林白蚁防治研究所 | Odontotermes formosanus is preparing the application in expediting the emergence of breast milk food |
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| CN1067555C (en) | 2001-06-27 |
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