CN1879701A - Cold-treating medicine and preparation method thereof - Google Patents
Cold-treating medicine and preparation method thereof Download PDFInfo
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- CN1879701A CN1879701A CN 200610035362 CN200610035362A CN1879701A CN 1879701 A CN1879701 A CN 1879701A CN 200610035362 CN200610035362 CN 200610035362 CN 200610035362 A CN200610035362 A CN 200610035362A CN 1879701 A CN1879701 A CN 1879701A
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Abstract
The invention discloses a medicament for treating common cold, which is prepared from flavoring agents, sweet wormwood, magnolia bark and baikal skullcap root as raw material through the steps of chopping, disintegrating, steam distilling, carbon dioxide extracting, water grilling, mixing with other pharmaceutically acceptable auxiliary materials. The medicament can be made into the dosage forms of capsule, drop pill preparation or other conventional medicaments.
Description
Technical field
The present invention relates to a kind of is the Chinese patent medicine that raw material is made with the Chinese crude drug, is specifically related to a kind of medicine for the treatment of flu and preparation method thereof.
Background technology
Flu is one of human normal disease of sending out, brings inconvenience and misery to people.According to the tcm treatment according to syndrome differentiation theory, flu mainly is divided into anemofrigid cold, anemopyretic cold and summer-heat damp cold three major types.Wherein the clinical manifestation of summer-heat damp cold is different from general flu, and touching difficulty is more treated more thorny.For the treatment of summer-heat damp cold, western medicine and medical practitioners adopts the method for symptomatic treatment more, cures the symptoms, not the disease at present.With organic conception and determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs is that such disease of traditional Chinese medical herbal treatment of juche idea has special advantages.The Chinese patent medicine kind that can be used for treating summer-heat damp cold at present have Radix agastaches healthy energy capsule, the positive air water of Radix agastaches, wushicha granules, antitoxic bolus of honeysuckle flower and forsythia, rheocamphoradin, LIUYI SAN, cloud essence, four positive balls, liuhe dingzhong pills, day in tea, manna tea, kam-wo tea etc.Wherein Radix agastaches healthy energy preparation, rheocamphoradin etc. are comparatively commonly used, and curative effect is also good.But these kind ubiquity preparation process fall behind, and dose is big, take shortcomings such as inconvenience and effect be not ideal enough.The Chang Yong decoction close with we has Xiangru San, newly adds Xiangru Xin etc. clinically, because it has certain curative effect from ancient prescription or by the utilization of ancient prescription plus-minus, uses also more general clinically.There is patient's inconvenient problem with use in most untapped one-tenth Chinese patent medicine kind but these are write out a prescription, and range of application also is restricted.
At present because China's medical industry level of development is backward relatively, new technique and new technology are owing to reasons such as idea and objective condition are difficult to popularize, with the Chinese crude drug is the medicine of feedstock production treatment flu, mainly be the active component that adopts in decocting cooking method and the decoction and alcohol sedimentation technique extraction raw material, then the medicinal liquid that obtains directly used or be processed into required dosage form.Volatile position or water soluble part are difficult to keep in the active component of crude drug, that is to say that the active component in the raw material is difficult to farthest extract and keep.
Chinese medicine compound has the characteristics of himself, and is little as toxicity, can comprehensively alleviate various symptoms, stable curative effect etc., is particularly suited for the complicated and disease of outbreak repeatedly of the cause of disease.The successive dynasties Chinese medicine has accumulated abundant clinical experience in the treatment of flu, stayed classical name side." formulary of peaceful benevolent dispensary " is loaded with the following prescription of treatment flu: Herba Moslae 9g and Semen Lablab Album, each 6g of Cortex Magnoliae Officinalis, and the function dispelling summer-heat from superficies of the body, removing dampness for regulating stomach cures mainly the febrile disease of YIN type in summer card.We institute controls and levies is that the Xia Yue drink of enjoying the cool is cold, affection of exogenous wind-cold, and internal injury is due to wet.The Xia Yue sense is cold, the evil flesh table that stagnates, so see fever with aversion to cold, lossless, general pain is nose heave, wind and cold table diseases such as floating pulse.Present inventor's clinical practice finds that this side is better for the patient treatment effect of the heresy of experiencing cold-damp summer, but causing catching a cold for diseases caused by summer heat, the heat-damp in summer that showed is laid equal stress on or heat is poor greater than wet sign treatment specific aim, from the Basic Theories of Chinese Medicine angle analysis, be because lack reason in the side to the heat clearing away class medicine of the heresy of summer heat-resistant, and adopt the mode of water decoction to take it more than clinical, and be not developed to suitable Chinese patent medicine kind owing to will this side do not combine, cause the patient to use inconvenience with modern preparation.
Summary of the invention
The medicine that the purpose of this invention is to provide a kind of modified model treatment flu.
Another object of the present invention provides the preparation method of described treatment cold medicine.
A further object of the present invention provides the application of described medicine in preparation treatment cold medicine, particularly the application in preparation treatment summer-wet type common cold medicine.
Further purpose of the present invention provides the pharmaceutical composition of described treatment cold medicine.
Product of the present invention, its raw material is by the Chinese medicine Herba Moslae of relieving the exterior syndrome by diaphoresis and middle dampness removing, the Chinese medicine Herba Artemisiae Annuae of clearind deficient heat, removing heat from blood, expelling summer-heat, preventing the attack (or recurrence) of malaria, the Chinese medicine Cortex Magnoliae Officinalis of warming middle-JIAO, the therapeutic method to keep the adverse QI flowing downwards, dampness, expectorant and heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive baikal skullcap root are formed.
The medicine of treatment flu of the present invention is made by following weight portion raw material:
Herba Moslae 3～25 Herba Artemisiae Annuaes 3～20
Cortex Magnoliae Officinalis 3～25 Radix Scutellariaes 3～20
Its preferred weight part feedstock production proportioning is:
Herba Moslae 6～20 Herba Artemisiae Annuaes 6～18
Cortex Magnoliae Officinalis 3～15 Radix Scutellariaes 3～15
Its optimum weight part feedstock production proportioning is:
Herba Moslae 15 Herba Artemisiae Annuaes 12
Cortex Magnoliae Officinalis 6 Radix Scutellariaes 6
The preparation method of the medicine of treatment flu of the present invention is made up of the following step:
(1) Herba Moslae is broken into the segment of 1～2cm, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae powder are broken into fine powder;
(2) preparation of raw extract:
The preparation of a, Herba Moslae volatile oil: get the Herba Moslae segment, add 8～12 times in water, soak time 2～4 hours, 6～10 hours extraction times of steam distillation are promptly;
The preparation of b, Herba Artemisiae Annuae extract: get the Herba Artemisiae Annuae powder, temperature be 35～45 ℃, pressure 18～22mPa condition carbon dioxide supercritical extraction 3.5～4.5 hours promptly;
The preparation of c, Cortex Magnoliae Officinalis extract: get the Cortex Magnoliae Officinalis powder, temperature be 35～45 ℃, pressure be 22～28mPa condition carbon dioxide supercritical extraction 5.5～6.5 hours promptly;
The preparation of d, Radix Scutellariae extract: get Radix Scutellariae powder, add 8～10 times in water, extraction time 2～3 times, each 0.5～1.5 hour; Aqueous extract is concentrated into every ml is equivalent to crude drug 0.5～4g, regulate pH value to 1.0～2.0,80 ℃ insulation 20～90 minutes, left standstill the filtering solvent 8～36 hours with hydrochloric acid; Precipitate adds 2 times of water gagings and stirs evenly, and regulates pH value to 7.0 with 40% sodium hydroxide solution, adds equivalent ethanol, stirring and dissolving, filtering residue; Filtrate is regulated pH value to 1.0～2.0,60 ℃ insulation 20～90 minutes with hydrochloric acid, leaves standstill the filtering solvent 8～36 hours; Precipitate uses the washing with alcohol of 10 times of water gagings and 20%, 40%, 60% to pH value to 7.0 successively, waves most ethanol, and drying under reduced pressure promptly.
(3) the resulting Herba Moslae volatile oil of step (2) and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract are mixed promptly.
Contain Herba Moslae total phenols 1%～20% in the said method drug prepared, magnolia bark total-phenol 1%～30%, arteannuin 1%～30%, baicalin 5%～60%.Described Herba Moslae total phenols comprises thymol and carvacrol; Described magnolia bark total-phenol comprises honokiol and magnolol.
In the extraction process by water of the present invention, WATER AS FLOW MEDIUM adopts the usual practice of this area with the ratio of being carried raw material, and promptly the consumption unit of water is a volume unit, and the consumption unit of being carried raw material is a unit of weight.
The invention still further relates to the application of described medicine in the medicine of preparation treatment flu, particularly relate to the application in preparation treatment summer-wet type common cold medicine.
The pharmaceutical composition that application of the present invention is made up of Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract and medically acceptable adjuvant.
Described pharmaceutical composition is a kind of soft capsule preparation, and this soft capsule preparation is made up of Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract and an amount of vegetable oil, 3%～5% Cera Flava and a small amount of wetting agent.Its preparation method is, earlier with the mixture of the mixture of Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract and the adjuvant mixed by 1: 2.3, goes up the Zhanang machine again and suppresses and form.
Described pharmaceutical composition is a kind of dropping pill formulation, and this dropping pill formulation is mixed with PEG4000 and PEG6000 and formed by Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract.Its preparation method is, earlier mixture and the PEG4000 and the PEG6000 of Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract pressed 1: 0.5: 2.5 mixed, and fusion again splashes in the coolant methyl-silicone oil and makes.
Medicine of the present invention removes the Semen Lablab Album in " formulary of peaceful benevolent dispensary " contained prescription, adds antipyretic Herba Artemisiae Annuae and Radix Scutellariae.This prescription selects for use hot using warming therapy Herba Moslae with the monarch drug in relieving the exterior syndrome by diaphoresis and the middle dampness removing side of serving as.Medicine of the present invention adopts Herba Moslae diaphoresis therapeutic purposes to be to bring down a fever, and syndrome of summer-heat-dampness pents up because of wet wearing with hot glue, heat is difficult to because of recovery following sweating, anti-variable its card of giving birth to, the direct purpose of damp-heat syndrome diaphoresis mainly is the expulsion damp-stagnancy, the spread sheet sun, harmonizing the functional activities of vital QI, make the wet also change of gasification, the wet hot orphan that dispels then easily clears up, and this is the reason of " opening brake application ".Cortex Magnoliae Officinalis is arduous warm in nature, goes into taste two warps, the function promoting the circulation of QI to remove dampness, and removing food stagnancy removes full, is amusing to help the Herba Moslae removing dampness, and the power of stomach function regulating preventing or arresting vomiting is to use it for ministerial drug.At the characteristics that summer-heat is contained partially, adding Radix Scutellariae, Herba Artemisiae Annuae clearing away summer-heat expel the heat-evil, and cooperate Herba Moslae, Cortex Magnoliae Officinalis clearing away summer-heat and eliminating dampness, both have been ministerial drug, are again adjuvant drug.Radix Scutellariae is bitter dry cold in nature, heat clearing and damp drying, and intestines strengthening and diarrhea checking, one get the effect of power to help the monarch and his subjects' removing dampness to be amusing of the bitter dampness of its property; The two is got the merit of its kind clear damp-heat in middle-JIAO and removes the suspicion that anti-humidity hysteresis accumulates heat-transformation of a specified duration.In addition, Herba Artemisiae Annuae and Radix Scutellariae compatibility are separated in wet hot and suffocatingly thoroughly, get Herba Artemisiae Annuae and both can walk table, can go into few sun again and walk edema caused by disorder of QI, and the blood system of also can joining the army can be gone into and can be gone out, property that can clearly can be saturating; Radix Scutellariae is clearing heat in QI system warm both, can remove damp-heat in the spleen and stomach again, and damp after all in burnt taste.So both compatibilities can be separated upper, middle and lower three warmers hot and suffocating in wet thoroughly.
Medicine of the present invention has antiinflammatory, brings down a fever, pain relieving, antidiarrheal, antibiotic and antiviral effect, can comprehensively alleviate the especially various symptoms of summer-heat damp cold of catching a cold.
The present invention adopts the carbon dioxide supercritical fluid extraction method to extract the lipotropy effective active composition (volatile oil) of Herba Artemisiae Annuae, Cortex Magnoliae Officinalis respectively, the extraction efficiency height, no solvent residue toxicity, active skull cap components heat-sensitive ingredients are difficult for decomposing to be destroyed, and can keep the extract natural feature to greatest extent.
The effective ingredient volatile oil composition that the present invention is directed in the Herba Moslae has effumability, and the characteristics that can come out with steam distillation adopt steam distillation to extract the Herba Moslae medical material, and extraction process is simple, rationally, the extraction efficiency height; Have water miscible characteristics according to active component baicalin in the Radix Scutellariae, adopt earlier the decocting cooking method that it is extracted, after add acid and be incubated reduction baicalin molecule at a certain temperature.Leave standstill then and make baicalin precipitation, then will precipitate transfer to neutrality with alkali liquor after, add acid treatment more once, obtain the baicalin crude product by twice at last with Acid precipitation, carry acid-alkali treatment through water, extract the effective ingredient in the medical material to greatest extent, remove the impurity composition.
The component of medicine of the present invention is few, and preparation technology is very simple.Prescription is a support with tradition name side, and in conjunction with Chinese medical theory and clinical experience for many years, prescription is more scientific and reasonable, treats with strong points, evident in efficacy.
Below further specify beneficial effect of the present invention by concrete experiment:
Experiment one: the external bacteriostasis of medicine of the present invention
The medical material mixed extract is mixed with every ml with nutrient broth and contains crude drug amount 0.1g, 0.05g, 0.025g ... 0.0015g, 0.00075 totally eight drug level, every pipe total amount 1ml, steam sterilization.Streptococcic test still needed add 1% glucose in the sterilization medicinal liquid, streptococcus pneumoniae is then added 10% deactivation rabbit anteserum, husky Bao Shi culture fluid preparating liquid is then used in oidiomycetic test to white.
Contrast: the strain contrast adds test organisms for the culture medium that does not contain medicine; The medicine contrast is not for adding the medicinal liquid of test organisms; The medicinal QINGRE XIAOYANNING of positive control.
The test organisms liquid that each concentration pipe of every row's medicinal liquid and strain control tube add 1: 2000 respectively (8 hours cultures) 0.1ml cultivates 18 hours observed results for 37 ℃.24 hours cultures are then used in oidiomycetic test to white, and medicinal liquid adds behind the bacterium liquid 28 ℃ and cultivates 48 hours observed results.With the turbidity is that each pipe of index naked-eye observation has asepsis growth.
Judge minimal inhibitory concentration (MIC).MIC is meant the minimum drug level that complete inhibition test growth is contained.Experimental result sees Table 1.
The external bacteriostasis experiment of table 1 extract
| Medicine | Candida albicans | Staphylococcus aureus | Jia Xingrongxuexinglianqiujun | Group B streptococcus | Streptococcus pneumoniae | Bacillus enteritidis | Colon bacillus |
| The mixed extract QINGRE XIAOYANNING | 0.00125 0.025 | 0.1 0.1 | 0.0075 0.05 | 0.0125 0.025 | 0.0125 0.2 | 0.0125 0.2 | 0.025 0.2 |
Annotate: it is normal that strain contrasts each bacteria growing; Medicine contrast asepsis growth.
Medical material strain that mixed extract tries all has antibacterial action in various degree, and is between 0.00125g/ml～0.05g/ml, wherein stronger to the effect of staphylococcus aureus and beta hemolytic streptococcus to the MIC of each bacterium.
Experiment two: medicine of the present invention causes the influence of rat fever to yeast
Rat male and female half and half, body weight 180～220g, Fundamentals of Measurement body temperature under the rat rest state is then according to the basal body temperature random packet.Every group of rat administration at twice irritated for the first time stomach to behind the half-value dose, and every rat abdomen subcutaneous injection 9% yeast suspension behind the modeling 4h, is irritated stomach for the second time and given second half dosage.When 4h, 6h, 8h, 10h, survey rat anus temperature respectively once after modeling, deduct basal body temperature with the anus temperature of each time point after the modeling, effect is added up as bringing down a fever.The results are shown in Table 2.
Table 2 medicine causes the influence experiment of rat fever to yeast
| Group | Example number (n) | Dosage g/kg | The temperature lift-off value (x ± s) | |||
| 4h | 6h | 8h | 10h | |||
| Dosage group small dose group in the heavy dose of group of blank group aspirin | 10 9 9 10 9 | - 0.2 0.9 0.3 0.1 | -0.07±0.23 -0.51±0.73 0.06±0.66 -0.05±0.52 -0.07±0.64 | 1.01±0.56 -0.12± 0.63 ** 0.88±0.58 1.05±0.66 0.69±0.68 | 1.29±0.34 0.37±0.46 ** 1.17±0.50 1.33±0.59 1.16±0.39 | 1.29±0.36 0.33±0.65 ** 0.70±0.46 ** 0.85±0.54 * 0.88±0.41 * |
Last table result shows, big-and-middle small dose group rat anus temperature when 10h descends more with respect to the blank group, and difference has the significance meaning, points out this medicine that rat fever due to the yeast is had certain refrigeration function.
Experiment three: the analgesic activity of medicine of the present invention
The mice glacial acetic acid causes pain and turns round body experiment---mice male and female half and half, body weight 18-22g, random packet, difference gastric infusion (morphine group lumbar injection), after 1 hour (morphine is after half an hour), every mouse peritoneal is injected 0.6% glacial acetic acid 0.2mL, and mice is turned round the number of times of body in back 20 minutes of the record injection because of pain.The results are shown in Table 3.
Table 3 medicine is to the influence of mice hot plate experiment analgesic activity
| Group | Example number (n) | Dosage g/kg | Pain threshold (x ± s) | |||
| 30min | 60min | 90min | 120min | |||
| Dosage group small dose group in the heavy dose of group of blank group morphine group | 11 10 11 11 11 | - 0.002 0.9 0.3 0.1 | 23.1±5.6 33.2±9.8 ** 31.4±5.1 ** 24.0±9.9 21.2±8.7 | 20.1±3.7 25.5±8.9 25.2±4.1 ** 24.3±4.3 * 21.2±5.0 | 20.0±7.4 24.2±9.6 26.3±7.3 19.6±4.0 23.5±5.9 | 20.0±5.4 27.6±10.2 ** 26.6±9.2 ** 20.3±4.9 * 18.2±7.4* |
Last table result shows that heavy dose of group is at 30min, and during 60min, small dose group is when 60min, and mice licks the metapedes number of times and is less than blank group, and difference has the significance meaning, and experiment has certain analgesic activity to the mice hot plate to point out this medicine.
Mice hot plate analgesic experiment---female mice, body weight 18-22g.During experiment water bath is regulated 55 ± 1 ℃ of constant temperature, put a large beaker in water, mice is dropped into beaker, the record mice is from dropping into beaker to the time that the pain of being heated is licked metapedes, as the pain threshold of this Mus.The screening pain threshold press pain threshold stratified random grouping the 10-30 mice of second, and the pain threshold when respectively organizing after the administration 30,60,90 and 120 minutes is write down in perfusion respectively.The results are shown in Table 4.
Table 4 medicine causes the experiment that mice pain is turned round the body influence to glacial acetic acid
| Group | Example number (n) | Dosage g/kg | Turn round body number of times (x ± s) |
| Dosage group small dose group in the heavy dose of group of blank group morphine group | 15 14 15 15 15 | - 0.002 0.9 0.3 0.1 | 39.5±20.8 7.4±7.0 ** 20.7±13.1 * 13.9±10.69 ** 21.8±18.6 ** |
Last table table result shows that big-and-middle small dose group mouse writhing number of times all is less than blank group, and difference has the significance meaning, further points out this medicine that the mice pain due to the glacial acetic acid is had certain analgesic activity.
Experiment four: the anti-diarrhea effect of medicine of the present invention
The experiment of medicine antidiarrheal adopts Folium Sennae to cause the diarrhea of mouse model.Mice is female, and body weight 18-22g is by the grouping of body weight stratified random.Irritate stomach and be subjected to reagent after 1 hour, every mouse stomach 10% Folium Sennae (30mL/kg body weight).Observe to 4 hours mice passage of loose stools number of times behind the Folium Sennae.The results are shown in Table 5.
Table 5 medicine is to the influence of Folium Sennae induced mice diarrhoea experiment
| Group | Example number (n) | Dosage g/kg | The loose stool number of times (x ± s) |
| Dosage group small dose group in the heavy dose of group of blank group berberine group | 12 12 12 12 12 | - 0.225 0.9 0.3 0.1 | 8.8±2.5 0.4±1.2 6.0±3.0 * 11.0±3.9 8.9±1.8 |
Last table result shows that heavy dose of group mice passage of loose stools number of times is less than blank group, and difference has the significance meaning, and the prompting medicine has anti-diarrhea effect to Folium Sennae induced mice diarrhoea.
Experiment five: the preventing or arresting vomiting effect of medicine of the present invention
The experiment of medicine preventing or arresting vomiting adopts copper sulfate to cause pigeon vomiting model.Pigeon body weight 300-400g, fasting be can't help water 16 hours, random packet.Blank group is irritated the stomach distilled water, and the positive drug group is irritated metoclopramide, and Chinese drug-treated group is irritated medicine of the present invention.Every pigeon gavages copper-bath by 0.4g/kg after 1 hour.The number of times of pigeon vomiting in 1.5 hours behind the record filling copper sulfate.The experiment of medicine preventing or arresting vomiting adopts copper sulfate to cause pigeon vomiting model, the results are shown in following table 6.
Table 6 medicine causes the influence of pigeon vomiting to copper sulfate
| Group | Dosage (g/kg) | Number of animals (only) | The vomiting number of times (x ± s) |
| Dosage group small dose group in the heavy dose of group of distilled water group metoclopramide group | -- 0.03 0.9 0.3 0.1 | 10 10 10 10 12 | 6.9±3.0 3.0±1.6 ** 4.2±2.1 * 5.4±2.1 6.0±2.4 |
Last table result shows, heavy dose of group pigeon vomiting number of times is less than blank group, and difference has the significance meaning, points out this medicine that pigeon vomiting due to the copper sulfate is had the preventing or arresting vomiting effect.
Experiment six: the antiinflammatory action of medicine of the present invention
Mice caused by dimethylbenzene xylene ear swelling model is adopted in the experiment of medicine antiinflammatory.Mice male and female half and half, body weight 18-22g, random packet, (Dexamethasone group was irritated distilled water to gastric infusion in 3 days, irritated the stomach dexamethasone in the 3rd day), after the last administration 1 hour, the dimethylbenzene of 50 μ L evenly is applied to the mouse right ear two sides causes inflammation, left ear is done contrast, put to death mice after 20 minutes, with the card punch of diameter 6mm ears being downcut with the position homalographic, weigh respectively with electronic balance, is the swelling degree with the difference of left and right sides auricle weight.The results are shown in Table 7.
Table 7 medicine xylol causes the experiment of mice ear influence
| Group | Dosage (g/kg) | Number of animals (only) | Left and right sides ear weight difference (x ± s) |
| Dosage group small dose group in the heavy dose of group of distilled water group Dexamethasone group | -- 0.004 0.9 0.3 0.1 | 13 12 12 12 11 | 3.84±2.52 1.78±2.31 * 1.53±1.04 * 2.62±1.67 4.45±3.88 |
Last table result shows that heavy dose of group mice swelling degree is organized less than blank, and difference has the significance meaning, points out this medicine xylol induced mice acute inflammation that anti-acute inflammation effect is arranged.
The specific embodiment
Mode below by embodiment further specifies the present invention, does not therefore limit the present invention within the scope of these embodiment.The model that supercritical carbon dioxide extraction described in following examples, used device are the manufacturing of Chinese dimension (HANDWAY) company is the supercritical carbon dioxide extraction device of 100ml-SPE.Raw materials used: Herba Moslae originates in Jiangxi available from Fenyi County, Jiangxi medical material company; Cortex Magnoliae Officinalis, Herba Artemisiae Annuae radix scutellariae medicinal materials are available from peaceful medical material market, Guangzhou, and quality all meets 2005 editions regulations in the Chinese Pharmacopoeia, and wherein Cortex Magnoliae Officinalis originates in Sichuan; Shandong, the Herba Artemisiae Annuae place of production; Radix Scutellariae originates in Hebei.
Embodiment one
Prescription: Herba Moslae 2500g, Herba Artemisiae Annuae 2000g, Cortex Magnoliae Officinalis 1000g, Radix Scutellariae 1000g.Clean up with clear water, then respectively 46 ℃ of oven dry down.
Herba Moslae after the oven dry is cut into 1～2cm segment, and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis are ground into 40 order fine powders, and Radix Scutellariae powder is broken to 40 order fine powders.
Adopt steam distillation to extract volatile oil to the Herba Moslae medical material, soak time 3.8 hours adds 8 times of amounts of water, 9.5 hours extraction times.
The model that Herba Artemisiae Annuae powder after the pulverizing, Cortex Magnoliae Officinalis powder adopt Chinese dimension (HANDWAY) company to make respectively is that the supercritical carbon dioxide extraction device of 100ml-SPE extracts effective active matter, wherein the extraction temperature of Herba Artemisiae Annuae powder is 43 ℃, extracting pressure 20mPa, the extraction time is 4.4 hours; The extraction temperature of Cortex Magnoliae Officinalis powder is 36 ℃, extracting pressure 27mPa, and the extraction time is 5.2 hours.
Baical Skullcap Root water decocting method extracts and further acid-alkali treatment is refining, 8 times of amounts of amount of water, extraction time 2 times, each 0.6 hour.Aqueous extract is concentrated into every ml is equivalent to crude drug 1g, regulate pH value to 1.0 with hydrochloric acid, 80 ℃ are incubated 30 minutes, left standstill 12 hours, filter, precipitate adds 2 times of water gagings and stirs evenly, and regulates pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stirring makes dissolving, filters, and filtrate is regulated pH value to 1.0 with hydrochloric acid, 60 ℃ are incubated 30 minutes, left standstill 33 hours, the filtering solvent, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure promptly.
Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract are mixed, and wherein the quality percentage composition of main effective ingredient is: Herba Moslae total phenols 1.5%, magnolia bark total-phenol 29%, arteannuin 1.2%, baicalin 58%.Described Herba Moslae total phenols comprises thymol and carvacrol; Described magnolia bark total-phenol comprises honokiol and magnolol.
Each medicinal substances extract is mixed with 275g Oleum Arachidis hypogaeae semen, add 23g Cera Flava and 2.5g tween 80, the rotary Zhanang of reuse machine is pressed into 1000 soft capsules (0.5g/ grain).
The consumption of said preparation is every day twice, and is each 4, oral.
Embodiment two:
Get Herba Moslae 600g, Herba Artemisiae Annuae 2800g, Cortex Magnoliae Officinalis 600g, Radix Scutellariae 2800g and clean, then 42 ℃ of oven dry down.Herba Moslae after the oven dry is cut into 1～2cm segment, and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae all are ground into 40 order fine powders.
Herba Moslae is pressed embodiment one described method and extracts soak time 4 hours, 8 times of amounts of amount of water, 10 hours extraction times; Herba Artemisiae Annuae powder, Cortex Magnoliae Officinalis powder after pulverizing are carried out supercritical carbon dioxide extraction respectively, and wherein the extraction temperature of Herba Artemisiae Annuae powder is 37 ℃, extracting pressure 19mPa, the extraction time is 3.5 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 44 ℃, extracting pressure 26mPa, and the extraction time is 5.5 hours; Baical Skullcap Root water decocting method extracts and further acid-alkali treatment is refining.8 times of amounts of amount of water, extraction time 3 times, each 1.2 hours.Aqueous extract is concentrated into every ml is equivalent to crude drug 1.2g, regulate pH value to 2.0 with hydrochloric acid, 80 ℃ are incubated 30 minutes, left standstill 32 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 2.0 with hydrochloric acid, and 60 ℃ are incubated 40 minutes, left standstill 16 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Each medicinal substances extract is mixed with soybean oil 283g, add 15g Cera Flava and 2.5g tween 80, the rotary Zhanang of reuse machine is pressed into 1000 soft capsules (0.5g/ grain).
The consumption of said preparation is every day twice, and is each 4, oral.
Embodiment three:
Get Herba Moslae 3800g, Herba Artemisiae Annuae 700g, Cortex Magnoliae Officinalis 1500g, Radix Scutellariae 600g and clean, 50 ℃ of oven dry down.Then Herba Moslae is made 1～2cm segment, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae powder are broken to 40 order fine powders.
Herba Moslae is extracted soak time 2 hours, 8 times of amounts of amount of water, 7 hours extraction times by embodiment one described method; Herba Artemisiae Annuae powder, Cortex Magnoliae Officinalis powder after pulverizing are carried out supercritical carbon dioxide extraction respectively, and the extraction temperature of Herba Artemisiae Annuae powder is 38 ℃, extracting pressure 20mPa, the extraction time is 3.8 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 41 ℃, extracting pressure 26mPa, and the extraction time is 6 hours; Press embodiment one described method and extract Baical Skullcap Root, 9 times of amounts of amount of water, extraction time 2 times, each 1.2 hours.Aqueous extract is concentrated into every ml is equivalent to crude drug 1.4g, regulate pH value to 1.5 with hydrochloric acid, 80 ℃ are incubated 50 minutes, left standstill 14 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.5 with hydrochloric acid, and 60 ℃ are incubated 50 minutes, left standstill 14 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Each medicinal substances extract is mixed with 278g Oleum Arachidis hypogaeae semen, add 20g Cera Flava and 2.5g tween 80, the rotary Zhanang of reuse machine is pressed into 1000 soft capsules (0.5g/ grain).
The consumption of said preparation is every day twice, and is each 4, oral.
Embodiment four:
Herba Moslae 2200g, Herba Artemisiae Annuae 1500g, Cortex Magnoliae Officinalis 2100g, Radix Scutellariae 2000g clean, 55 ℃ of oven dry down.Pressing embodiment one then cuts off raw material and pulverize.
Adopt steam distillation to extract volatile oil, soak time 3 hours, 8 times of amounts of amount of water, 7 hours extraction times the Herba Moslae medical material; Herba Artemisiae Annuae powder, Cortex Magnoliae Officinalis powder after pulverizing are carried out supercritical carbon dioxide extraction respectively, and wherein the extraction temperature of Herba Artemisiae Annuae powder is 40 ℃, extracting pressure 22mPa, the extraction time is 3.5 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 45 ℃, extracting pressure 25mPa, and the extraction time is 6.5 hours; Press embodiment one described method and extract Baical Skullcap Root, 10 times of amounts of amount of water, extraction time 3 times, each 0.5 hour.Extracting solution is concentrated into every ml and is equivalent to crude drug 1.6g, regulates pH value to 1.6 with hydrochloric acid, and 80 ℃ are incubated 80 minutes, left standstill 24 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.6 with hydrochloric acid, and 60 ℃ are incubated 80 minutes, left standstill 30 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract are mixed, and wherein the quality percentage composition of main effective ingredient is: Herba Moslae total phenols 10%, magnolia bark total-phenol 1.5%, arteannuin 15%, baicalin 35%.Described Herba Moslae total phenols comprises thymol and carvacrol; Described magnolia bark total-phenol comprises honokiol and magnolol.
Medicinal substances extract is mixed with 100gPEG4000 and 500gPEG6000, and fusion splashes into and promptly gets (50mg/ grain) in the coolant methyl-silicone oil.
The consumption of said preparation is every day twice, and each 2.5g is oral.
Embodiment five:
Herba Moslae 1800g, Herba Artemisiae Annuae 1800g, Cortex Magnoliae Officinalis 800g, Radix Scutellariae 900g clean, 52 ℃ of oven dry down.Pressing embodiment one cuts off raw material and pulverize.
Herba Moslae is extracted soak time 3.5 hours, 10 times of amounts of amount of water, 8 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 42 ℃, extracting pressure 22mPa, and the extraction time is 4 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 42 ℃, extracting pressure 26mPa, the extraction time is 6 hours; Radix Scutellariae powder is extracted 9 times of amounts of amount of water, extraction time 3 times, each 1 hour by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 1.8g, regulate pH value to 1.4 with hydrochloric acid, 80 ℃ are incubated 90 minutes, left standstill 36 hours, filter, precipitate adds 2 times of water gagings and stirs evenly, and regulates pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stirring makes dissolving, filters, and filtrate is regulated pH value to 1.4 with hydrochloric acid, 60 ℃ are incubated 90 minutes, left standstill 36 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure promptly.
Medicinal substances extract is mixed with 100gPEG4000 and 500gPEG6000, and fusion splashes into and promptly gets (50mg/ grain) in the coolant methyl-silicone oil.
The consumption of said preparation is every day twice, and each 2.5g is oral.
Embodiment six:
Get Herba Moslae 2600g, Herba Artemisiae Annuae 2200g, Cortex Magnoliae Officinalis 2500g, Radix Scutellariae 1500g and clean, 58 ℃ of oven dry down.
Pressing embodiment one cuts off raw material and pulverize.
Herba Moslae is extracted soak time 2.1 hours, 11 times of amounts of amount of water, 6 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 44 ℃, extracting pressure 18mPa, and the extraction time is 4.4 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 45 ℃, extracting pressure 20mPa, the extraction time is 6.5 hours; Radix Scutellariae powder is extracted 9.5 times of amounts of amount of water, extraction time 2 times, each 0.6 hour by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 2.0g, regulate pH value to 1.8 with hydrochloric acid, 80 ℃ are incubated 70 minutes, left standstill 20 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.8 with hydrochloric acid, and 60 ℃ are incubated 70 minutes, left standstill 12 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Medicinal substances extract is mixed with 100gPEG4000 and 500gPEG6000, and fusion splashes into and promptly gets (50mg/ grain) in the coolant methyl-silicone oil.
The consumption of said preparation is every day twice, and each 2.5g is oral.
Embodiment seven:
Herba Moslae 3500g, Herba Artemisiae Annuae 2900g, Cortex Magnoliae Officinalis 3800g, Radix Scutellariae 3000g clean, and 60 ℃ of oven dry down.
Pressing embodiment one cuts off raw material and pulverize.
Herba Moslae is extracted soak time 3 hours, 11 times of amounts of amount of water, 6.5 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 45 ℃, extracting pressure 19mPa, and the extraction time is 3.7 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 37 ℃, extracting pressure 23mPa, the extraction time is 6.3 hours; Radix Scutellariae powder is extracted 10 times of amounts of amount of water, extraction time 2 times, each 1.4 hours by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 2.3g, regulate pH value to 1.2 with hydrochloric acid, 80 ℃ are incubated 60 minutes, left standstill 21 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.2 with hydrochloric acid, and 60 ℃ are incubated 40 minutes, left standstill 28 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Medicinal substances extract is mixed with 100gPEG4000 and 500gPEG6000, and fusion splashes into and promptly gets (50mg/ grain) in the coolant methyl-silicone oil.
The consumption of said preparation is every day twice, and each 2.5g is oral.
Embodiment eight:
Herba Moslae 600g, Herba Artemisiae Annuae 2600g, Cortex Magnoliae Officinalis 3600g, Radix Scutellariae 2300g clean, and 42 ℃ of oven dry are down pressed embodiment one then raw material is cut off or pulverizes.
Herba Moslae is extracted soak time 4 hours, 12 times of amounts of amount of water, 6 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 35 ℃, extracting pressure 20mPa, and the extraction time is 3.9 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 41 ℃, extracting pressure 23mPa, the extraction time is 5.7 hours; Radix Scutellariae powder is extracted 9.5 times of amounts of amount of water, extraction time 2 times, each 1.3 hours by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 2.6g, regulate pH value to 1.7 with hydrochloric acid, 80 ℃ are incubated 50 minutes, left standstill 36 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.7 with hydrochloric acid, and 60 ℃ are incubated 85 minutes, left standstill 28 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Medicinal substances extract is mixed with 100gPEG4000 and 500gPEG6000, and fusion splashes into and promptly gets (50mg/ grain) in the coolant methyl-silicone oil.
The consumption of said preparation is every day twice, and each 2.5g is oral.
Embodiment nine:
Herba Moslae 800g, Herba Artemisiae Annuae 2500g, Cortex Magnoliae Officinalis 3000g, Radix Scutellariae 2500g clean, and 46 ℃ of oven dry are down pressed embodiment one then raw material is cut off or pulverizes.
Herba Moslae is extracted soak time 4 hours, 12 times of amounts of amount of water, 6 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 35 ℃, extracting pressure 20mPa, and the extraction time is 3.9 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 41 ℃, extracting pressure 23mPa, the extraction time is 5.7 hours; Radix Scutellariae powder is extracted 9.5 times of amounts of amount of water, extraction time 2 times, each 1.3 hours by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 2.8g, regulate pH value to 1.1 with hydrochloric acid, 80 ℃ are incubated 50 minutes, left standstill 36 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.1 with hydrochloric acid, and 60 ℃ are incubated 85 minutes, left standstill 28 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Herba Moslae, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis extract are used the beta-cyclodextrin inclusion compound of 1.5 times of amounts respectively, clathrate mixes with Radix Scutellariae extract again, adds 150g starch, mixes, after 80 mesh sieves 2 times, put in the blender, the adding distilled water is an amount of, stirs 10 minutes, the system soft material, cross 16 mesh sieves and granulate, wet grain is dry in 60 ℃ of air dry ovens, and dry granular is crossed 16 mesh sieve granulate.Add carboxymethyl starch sodium 25g and magnesium stearate 5g, mix homogeneously, tabletting, the bag film-coat, promptly.
The consumption of said preparation is every day three times, and is each 2, oral.
Embodiment ten:
Herba Moslae 3000g, Herba Artemisiae Annuae 1500g, Cortex Magnoliae Officinalis 3500g, Radix Scutellariae 1800g clean, and 50 ℃ of oven dry down.
Pressing embodiment one cuts off raw material and pulverize.
Herba Moslae is extracted soak time 3 hours, 11 times of amounts of amount of water, 6.5 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 45 ℃, extracting pressure 19mPa, and the extraction time is 3.7 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 37 ℃, extracting pressure 23mPa, the extraction time is 6.3 hours; Radix Scutellariae powder is extracted 10 times of amounts of amount of water, extraction time 2 times, each 1.4 hours by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 3.0g, regulate pH value to 1.9 with hydrochloric acid, 80 ℃ are incubated 60 minutes, left standstill 21 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.9 with hydrochloric acid, and 60 ℃ are incubated 40 minutes, left standstill 28 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Herba Moslae, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis extract are used the beta-cyclodextrin inclusion compound of 1.5 times of amounts respectively, clathrate mixes with Radix Scutellariae extract again, adds 150g starch, mixes, after 80 mesh sieves 2 times, put in the blender, the adding distilled water is an amount of, stirs 10 minutes, the system soft material, cross 16 mesh sieves and granulate, wet grain is dry in 60 ℃ of air dry ovens, and dry granular is crossed 16 mesh sieve granulate.Add carboxymethyl starch sodium 25g and magnesium stearate 5g, mix homogeneously, tabletting, the bag film-coat, promptly.
The consumption of said preparation is every day three times, and is each 2, oral.
Embodiment 11:
Get Herba Moslae 1700g, Herba Artemisiae Annuae 2800g, Cortex Magnoliae Officinalis 1300g, Radix Scutellariae 1600g and clean, 47 ℃ of oven dry down.
Pressing embodiment one cuts off raw material and pulverize.
Herba Moslae is extracted soak time 2.1 hours, 11 times of amounts of amount of water, 6 hours extraction times by embodiment one described method; By embodiment one described method Herba Artemisiae Annuae powder magnolia obovata powder is extracted, wherein the extraction temperature of Herba Artemisiae Annuae powder is 44 ℃, extracting pressure 18mPa, and the extraction time is 4.4 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 45 ℃, extracting pressure 20mPa, the extraction time is 6.5 hours; Radix Scutellariae powder is extracted 9.5 times of amounts of amount of water, extraction time 2 times, each 0.6 hour by embodiment one described method.Aqueous extract is concentrated into every ml is equivalent to crude drug 3.8g, regulate pH value to 1.3 with hydrochloric acid, 80 ℃ are incubated 70 minutes, left standstill 20 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.3 with hydrochloric acid, and 60 ℃ are incubated 70 minutes, left standstill 12 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
Respectively with the beta-cyclodextrin inclusion compound of 2 amounts, clathrate mixes with Radix Scutellariae extract again, adds 100g starch with Herba Moslae, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis extract, mix,, put in the blender after 80 mesh sieves 2 times, it is an amount of to add distilled water, stirred 10 minutes, the system soft material is crossed 16 mesh sieves and is granulated, wet grain is dry in 60 ℃ of air dry ovens, dry granular is crossed 16 mesh sieve granulate, divides packing, promptly gets granule.
The consumption of said preparation is every day 2 times, each 1 bag (2g), and warm water is taken after mixing it with water.
Embodiment 12:
Herba Moslae 1500g, Herba Artemisiae Annuae 900g, Cortex Magnoliae Officinalis 1700g, Radix Scutellariae 2100 are cleaned, 57 ℃ of oven dry down.Pressing embodiment one then cuts off raw material and pulverize.
Adopt steam distillation to extract volatile oil, soak time 3 hours, 8 times of amounts of amount of water, 7 hours extraction times the Herba Moslae medical material; Herba Artemisiae Annuae powder, Cortex Magnoliae Officinalis powder after pulverizing are carried out supercritical carbon dioxide extraction respectively, and wherein the extraction temperature of Herba Artemisiae Annuae powder is 40 ℃, extracting pressure 22mPa, the extraction time is 3.5 hours, the extraction temperature of Cortex Magnoliae Officinalis powder is 45 ℃, extracting pressure 25mPa, and the extraction time is 6.5 hours; Press embodiment one described method and extract Baical Skullcap Root, 10 times of amounts of amount of water, extraction time 3 times, each 0.5 hour.Aqueous extract is concentrated into every ml is equivalent to crude drug 3.5g, regulate pH value to 1.5 with hydrochloric acid, 80 ℃ are incubated 70 minutes, left standstill 24 hours, and filtered, precipitate adds 2 times of water gagings and stirs evenly, regulate pH value to 7.0 with 40% sodium hydroxide solution, add equivalent ethanol, stir and make dissolving, filter, filtrate is regulated pH value to 1.5 with hydrochloric acid, and 60 ℃ are incubated 80 minutes, left standstill 30 hours, and filtered, precipitation is washed till pH value to 7.0 with the ethanol of 10 times of water gagings and 20%, 40%, 60% successively, wave most ethanol, drying under reduced pressure.
After above-mentioned each extract mixed, the quality percentage composition of main effective ingredient was: Herba Moslae total phenols 18%, magnolia bark total-phenol 15%, arteannuin 29%, baicalin 6%.Described Herba Moslae total phenols comprises thymol and carvacrol; Described magnolia bark total-phenol comprises honokiol and magnolol.
With Herba Moslae, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis extract respectively with 2 the amount beta-cyclodextrin inclusion compounds, clathrate mixes with Radix Scutellariae extract again, adds 100g starch, mixes, after 80 mesh sieves 2 times, put in the blender, the adding distilled water is an amount of, stirs 10 minutes, the system soft material, cross 16 mesh sieves and granulate, wet grain is dry in 60 ℃ of air dry ovens, and dry granular is crossed 16 mesh sieve granulate.Incapsulate and promptly get hard capsule in the shell.
The consumption of said preparation is every day 3 times, and is each 2, oral.
Claims (10)
1, a kind of medicine for the treatment of flu, make by following weight portion raw material:
Herba Moslae 3～25 Herba Artemisiae Annuaes 3～20
Cortex Magnoliae Officinalis 3～25 Radix Scutellariaes 3～20.
2, according to the medicine of the described treatment flu of claim 1, make by following weight portion raw material:
Herba Moslae 6～20 Herba Artemisiae Annuaes 6～18
Cortex Magnoliae Officinalis 3～15 Radix Scutellariaes 3～15.
3, according to the medicine of the described treatment flu of claim 2, make by following weight portion raw material:
Herba Moslae 15 Herba Artemisiae Annuaes 12
Cortex Magnoliae Officinalis 6 Radix Scutellariaes 6.
4, the preparation method of claim 1,2 or 3 described treatment cold medicines is made up of the following step:
(1) Herba Moslae is broken into the segment of 1～2cm, Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae powder are broken into fine powder;
(2) preparation of raw extract:
The preparation of a, Herba Moslae volatile oil: get the Herba Moslae segment, add 8～12 times in water, soak time 2～4 hours, 6～10 hours extraction times of steam distillation are promptly;
The preparation of b, Herba Artemisiae Annuae extract: get the Herba Artemisiae Annuae powder, temperature be 35～45 ℃, pressure 18～22mPa condition carbon dioxide supercritical extraction 3.5～4.5 hours promptly;
The preparation of c, Cortex Magnoliae Officinalis extract: get the Cortex Magnoliae Officinalis powder, temperature be 35～45 ℃, pressure be 22～28mPa condition carbon dioxide supercritical extraction 5.5～6.5 hours promptly;
The preparation of d, Radix Scutellariae extract: get Radix Scutellariae powder, add 8～10 times in water, extraction time 2～3 times, each 0.5～1.5 hour; Aqueous extract is concentrated into every ml is equivalent to crude drug 0.5～4g, regulate pH value to 1.0～2.0,80 ℃ insulation 20～90 minutes, left standstill the filtering solvent 8～36 hours with hydrochloric acid; Precipitate adds 2 times of water gagings and stirs evenly, and regulates pH value to 7.0 with 40% sodium hydroxide solution, adds equivalent ethanol, stirring and dissolving, filtering residue; Filtrate is regulated pH value to 1.0～2.0,60 ℃ insulation 20～90 minutes with hydrochloric acid, leaves standstill the filtering solvent 8～36 hours; Precipitate uses the washing with alcohol of 10 times of water gagings and 20%, 40%, 60% to pH value to 7.0 successively, waves most ethanol, and drying under reduced pressure promptly.
(3) the resulting Herba Moslae volatile oil of step (2) and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract is mixed.
5, claim 1, the application of 2 or 3 described medicines in the medicine of preparation treatment flu.
6, the described application of claim 5 is characterized in that the application of described medicine in the medicine of preparation treatment summer-wet type common cold.
7, the described application of claim 6 is characterized in that the pharmaceutical composition of being made up of Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract and medically acceptable adjuvant.
8, the described pharmaceutical composition of claim 7 is the capsule preparations that contains Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract.
9, the described pharmaceutical composition of claim 7 is the dropping pill formulation that contains Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract.
10, the described pharmaceutical composition of claim 7 is the granule that contains Herba Moslae volatile oil and Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Radix Scutellariae extract.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306080B (en) * | 2007-05-17 | 2011-09-21 | 厦门金日制药有限公司 | Elsholtzia spray |
| CN104784306A (en) * | 2014-01-20 | 2015-07-22 | 中国中医科学院中药研究所 | Traditional Chinese medicinal compound preparation for treating colds |
| CN104825972A (en) * | 2015-05-25 | 2015-08-12 | 沈阳市清巢堂生物科技有限公司 | Vinegar-fried traditional Chinese medicine submicron powder preparation for preventing and treating livestock plague |
| CN111249421A (en) * | 2020-03-30 | 2020-06-09 | 杭州鸿育医药科技有限公司 | Traditional Chinese medicine preparation for treating initial damp-heat exogenous fever as well as preparation method and application thereof |
-
2006
- 2006-05-08 CN CN200610035362A patent/CN100581560C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306080B (en) * | 2007-05-17 | 2011-09-21 | 厦门金日制药有限公司 | Elsholtzia spray |
| CN104784306A (en) * | 2014-01-20 | 2015-07-22 | 中国中医科学院中药研究所 | Traditional Chinese medicinal compound preparation for treating colds |
| CN104825972A (en) * | 2015-05-25 | 2015-08-12 | 沈阳市清巢堂生物科技有限公司 | Vinegar-fried traditional Chinese medicine submicron powder preparation for preventing and treating livestock plague |
| CN104825972B (en) * | 2015-05-25 | 2018-07-27 | 辽宁阔安生物科技有限公司 | A kind of vinegar leaching Chinese herbal ultra-fine powder preparation of prevention farm animal and bird plague |
| CN111249421A (en) * | 2020-03-30 | 2020-06-09 | 杭州鸿育医药科技有限公司 | Traditional Chinese medicine preparation for treating initial damp-heat exogenous fever as well as preparation method and application thereof |
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