CN101612316B - Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy - Google Patents
Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy Download PDFInfo
- Publication number
- CN101612316B CN101612316B CN2009101011375A CN200910101137A CN101612316B CN 101612316 B CN101612316 B CN 101612316B CN 2009101011375 A CN2009101011375 A CN 2009101011375A CN 200910101137 A CN200910101137 A CN 200910101137A CN 101612316 B CN101612316 B CN 101612316B
- Authority
- CN
- China
- Prior art keywords
- dendrobium officinale
- aqueous extract
- herba dendrobii
- officinale aqueous
- apoplexy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an application of dendrobium officinale, aiming at disclosing an application of a dendrobium officinale aqueous extract in preparing a medicine for preventing or treating hypertension and apoplexy. The invention also provides dipping paste, particles, capsules, tablets and oral liquid which are suitable for the novel application and are prepared by utilizing the dendrobium officinale aqueous extract and relevant preparation methods. The applied dendrobium officinale aqueous extract has a moderate and permanent function of lowering blood pressure of a white rat with hereditary apoplexy type spontaneous hypertension and can prolong the survival time thereof, enhance the survival rate and prevent apoplexy, and the relevant medicine effect function has a certain relativity with the use quantity of the dendrobium officinale aqueous extract. The dendrobium officinale aqueous extract is utilized as a medicine with a specific application, can lower the blood pressure of a patient with hypertension moderately and permanently, delays the evolution of hypertension class cerebrovascular injuries and is of great significance for human beings to prevent the hypertensionand the apoplexy and lower the mutilation rate and the fatal rate of the hypertension.
Description
Technical field
The present invention relates to the purposes of Herba Dendrobii, relate in particular to the application of dendrobium officinale aqueous extract in the medicine for preparing prevention or treatment hypertension, apoplexy.
Background technology
Hypertension is common high morbidity, and prevalence is ascendant trend year by year, at present existing about 200,000,000 people of hyperpietic of China.With the quickening pace of modern life, the change of diet structure, hypomotility, its age of onset shifts to an earlier date year by year, and sickness rate further rises.Hypertension often makes important vital organs such as brain, the heart, kidney impaired along with PD, functional change and organic lesion take place, even it is deadly to disable, and apoplexy promptly is one of serious hypertensive cerebral organ injury.Present prevent and treat in the hypertension, in the western drug, in controlling blood pressure, still do not have the medicine that effectively stops secondary affections progress such as brain, the heart, kidney, thus the serious secondary affection of cerebrovascular---the generation of apoplexy clinically, and become that the hypertension secondary affection is disabled and death in the first place.
Herba Dendrobii is a rare or endangered species protection kind, is YIN nourishing and the production of body fluid promoting Chinese medicine material, and NATURAL DISTRIBUTION is in the ground such as Zhejiang, Anhui, Fujian, Hunan, Guangxi, Sichuan and Yunnan of China.Because the Herba Dendrobii medical value is high, wild resource is rare, and through unrestricted, predatory and destructive excavating for many years, wild resource has been on the verge of exhaustion.Tianhuang Pharmaceutical Co., Ltd., Zhejiang has thousands of mu of artificial culture bases, for carrying out standardized study and good basis has been laid in product development.But the Herba Dendrobii hyoscine sees the Shennong's Herbal of the Eastern Han Dynasty the earliest, apart from the present nearly 2,000 years.Herba Dendrobii is as a kind of of Herba Dendrobii and hyoscine, according to textual criticism also only surplus in the of 200 year.Li Shizhen (1518-1593 A.D.) is put down in writing the function of Herba Dendrobii and is act as " controlling the heating spontaneous perspiration, plug in the carbuncle evacuation of pus " in his Compendium of Material Medica.The famous physician Li Zhongzi of the Ming Dynasty says in " explaining abstruseness of the canon of materia medica ": " Herba Dendrobii, the Gan Keyue spleen, salty can kidney tonifying, so many merits are dirty in water and soil two, but gas is wide slow, the merit that no victory is played, ancients with this tea, very clearly on the diaphragm in generation." to the Qing Dynasty, ZHAO Xue-Min is included the Herba Dendrobii effect and is " clearing stomach removes deficiency-heat, promotes the production of body fluid " in his supplementary Amplifications of the Compendium of Materia Medica.Yet other traditional pharmacopeia and modern document are not seen the record that blood pressure lowering and prevention of brain apoplexy function are arranged.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of new purposes of Herba Dendrobii is provided, be i.e. the application of dendrobium officinale aqueous extract in the medicine of preparation prevention, treatment hypertension or apoplexy.Further object of the present invention provides to being suitable for new purposes and utilizes extractum, granule, capsule, tablet, oral liquid and the related manufacturing processes of dendrobium officinale aqueous extract preparation.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
The present invention has disclosed the application of dendrobium officinale aqueous extract in the medicine of preparation prevention, treatment hypertension or apoplexy.
As further purpose, the invention provides the Herba Dendrobii extractum of a kind of prevention or treatment hypertension, apoplexy, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation extractum are used, wherein the mass content of active component dendrobium officinale aqueous extract is 8.0%～80%.
The present invention also provides a kind of prevention or has treated the Herba Dendrobii granule of hypertension, apoplexy, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation granule are used, wherein the mass content of active component dendrobium officinale aqueous extract is 2.0%～80%.
The present invention also provides a kind of prevention or has treated the Herba Dendrobii capsule of hypertension, apoplexy, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation capsule are used, wherein the mass content of active component dendrobium officinale aqueous extract is 10%～50%.
The present invention also provides a kind of prevention or has treated the Herba Dendrobii tablet of hypertension, apoplexy, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation tablet are used, wherein the mass content of active component dendrobium officinale aqueous extract is 10%～50%.
The present invention also provides a kind of prevention or has treated the Herba Dendrobii oral liquid of hypertension, apoplexy, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation oral liquid are used, wherein the mass content of active component dendrobium officinale aqueous extract is 0.1%～16%.
The present invention further provides a kind of prevention or has treated the Herba Dendrobii extract making method of hypertension, apoplexy, may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into 0.1～2.0 gram crude drug/gram [10%～200%]; Perhaps extracting solution is concentrated into relative density 1.000～1.150, in acceptable accessories refined honey, maltose, sucrose or the Mel that adding preparation extractum or taste cream are used one or more, the mass content that makes the active component dendrobium officinale aqueous extract is 8.0%～80%, mix homogeneously is 300,000 grades of clean area fills;
(4) semi-finished product are put to sterilize in the sterilization cabinet and are promptly got Herba Dendrobii extractum finished product.
The present invention also provides a kind of prevention or has treated the preparation method of the Herba Dendrobii granule of hypertension, apoplexy, may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) the extraction concentrated solution mixes one or more in acceptable accessories glucose, sucrose, starch, the cellulose, the mass content that makes the active component dendrobium officinale aqueous extract is 2.0%～80%, spray granulation, packing, irradiation-sterilize promptly gets Herba Dendrobii granule finished product.
The present invention also provides a kind of prevention or has treated the capsular preparation method of Herba Dendrobii of hypertension, apoplexy, may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories or medicated powder to make the mass content of active component dendrobium officinale aqueous extract be 10%～50%, spray granulation divides the capsule of packing into, and irradiation-sterilize promptly gets the Herba Dendrobii capsule finished product.
The present invention also provides a kind of prevention or has treated the preparation method of the Herba Dendrobii tablet of hypertension, apoplexy, may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) the extracting solution concentrated solution mixes one or more in acceptable accessories glucose, sucrose, starch or the cellulose, and the mass content that makes the active component dendrobium officinale aqueous extract is 10%～50%, spray granulation, tabletting, packing, irradiation-sterilize promptly gets Herba Dendrobii tablet finished product.
The present invention also provides a kind of prevention or has treated the preparation method of the Herba Dendrobii oral liquid of hypertension, apoplexy, may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories flavoring agent or antiseptic to make the mass content of active component dendrobium officinale aqueous extract be 0.1%～16%, packing, sterilization promptly gets Herba Dendrobii oral liquid finished product.
Among the present invention, described crude drug is meant the medical material Herba Dendrobii; The active component that contains 20～40% (mass parts) in every gram Herba Dendrobii crude drug---the dendrobium officinale aqueous extract that obtains through extraction process by water; The quality of dendrobium officinale aqueous extract is meant the quality of solid content under 105 ℃ constant weight condition of dendrobium officinale aqueous extract.
The invention has the beneficial effects as follows:
The application of dendrobium officinale aqueous extract has the persistent effect of bringing high blood pressure down of mitigation to the easy apoplectic type spontaneous hypertension rat of heritability (SHR-sp), can prolong life span, raising survival rate, the prevention of stroke of SHR-sp, relevant drug action and its consumption have certain dependency.It as the utilization of medicine of special-purpose, can be relaxed persistent reductions hyperpietic blood pressure, delay hypertensive cerebral cerebrovascular infringement progress, to human prophylaxis of hypertension apoplexy, the disabling of reduction hypertension, fatality rate are significant.
The specific embodiment
One, zooperal material and method:
1. dendrobium officinale aqueous extract: provided by Tianhuang Pharmaceutical Co., Ltd., Zhejiang, lot number is: 070612,070620,070717.
2. hypertension animal model rat: adopt easy apoplectic type spontaneous hypertensive rat (SHR-sp).SHR-sp hypertension pathology characteristics are similar to the severe hypertension of human body, and PD is very fast, easily apoplexy.SHR-sp gets through close relative's assortative mating from the SHR filial generation of dying from apoplexy, and apoplexy can take place in 9～13 months to need not to add inducement (full diet raising), and incidence rate can be up to 90%.The pathological change of development takes place with human closely similar in its hypertension and apoplexy, can better react the situation of drug effect in human body.Above-mentioned rat model is provided by Chinese Academy of Sciences's Shanghai animal center, and each treated animal begins experiment 10 ages in week, the male and female dual-purpose, and cleaning level (SPF) Animal House is raised.
3. grouping and treatment:
Dosage group, Herba Dendrobii small dose group, Western medicine nimodipine group, model water matched group, intact animal wistar matched group in the heavy dose of group of Herba Dendrobii, the Herba Dendrobii, totally 6 groups, every group of 20～30 animals, male and female dual-purpose are established in experiment.Below respectively organize 1 continuous 12 week of gastric infusion of equal every day.The treatment of 12 weeks finishes the back drug withdrawal, continues to observe for 45 weeks (observe 36 weeks of blood pressure, after stopping to measure blood pressure, continue to observe existence 9 weeks of situation to 45 weeks), stops experiment.It is as follows that each organizes rat oral gavage medicine for treatment dosage:
The heavy dose of group of Herba Dendrobii: 1.0g crude drug/Kg body weight/day;
Dosage group in the Herba Dendrobii: 0.5g crude drug/Kg body weight/day;
Herba Dendrobii small dose group: 0.25g crude drug/Kg body weight/day;
Western medicine nimodipine group: 20mg/Kg body weight/day;
SHR-sp model control group: wait capacity normal saline/Kg body weight/day;
Intact animal wistar matched group: wait capacity normal saline/Kg body weight/day.
4. main observed content and method:
This research is main to be observed and respectively organizes rat blood pressure, general weather, apoplexy situation [comprise apoplexy time of origin, apoplexy a situation arises, apoplexy incidence rate, survive natural law, apoplexy mortality rate (survival ratio)] after 12 weeks of treatment and the drug withdrawal 45 weeks, in addition heart, brain, kidney weight is measured.
(1) blood pressure measuring method: the ALC-NIBP noinvasive arteria caudalis blood pressure determination analytical system that adopts Shanghai Alcott bio tech ltd to produce.This system blocks the arteria caudalis pulse with highly sensitive pulse transducer monitoring animal arteria caudalis pulse signal with aerating gasbag, and the synchronous change by check and analysis gasbag pressure and pulse signal records blood pressure.This system advantage is that animal need not anaesthetize, not have wound, and is easy to operate, and systolic pressure, diastolic pressure, mean pressure and the heart rate of energy accurate recording, demonstration and printing animal.In addition, this system applies computer sampling, automatic data processing and pulse-pressure synchronizing pattern, and can store and add up interior experimental result of regular period, and can store and recur measurement data, pulse-pressure figure easily, be applicable to macrocyclic blood pressure determination experiment.Before the experiment and in the experiment, measure blood pressure weekly 1 time, carry out rat arteria caudalis insulation (37 ℃) before each the measurement and in measuring.When every animal is measured blood pressure, measure 3 times at every turn, average.Measure the blood pressure time: each is organized after the medicine for treatment 1.5 hours ± 10 minutes and measures.
(2) apoplexy situation: observe respectively organize the rat apoplexy a situation arises, apoplexy time of origin, apoplexy incidence rate, existence natural law, apoplexy mortality rate (survival ratio), dissect internal organs (apoplexy) situation of checking.
(3) heart, brain, the kidney amount of thinking highly of and weight ratio thereof: observe and write down the body weight and the heart, brain, the kidney amount of thinking highly of when animal dead or execution, calculate its internal organs/weight ratio.
(4) general weather (body weight): observe and write down situations such as dietary amount, urine amount, stool, hair color, activity (irritability of suddenly making an uproar), body weight.
5. statistical method: measurement data t check, enumeration data X
2Check.45 weeks existence natural law employing F check after 36 all blood pressures, the drug withdrawal after the drug withdrawal.
Two, zooperal result:
1. blood pressure:
The large, medium and small dosage group of Herba Dendrobii is treated the SHR-sp rat oral gavage all had certain hypotensive effect in 12 weeks.Treat and 1 week began to have certain effect, treat after 6 weeks comparatively remarkable.The large, medium and small dosage group of Herba Dendrobii is best with middle dosage group hypotensive effect.Treatment is during 6 weeks, and dosage group blood pressure 210.03 ± 20.07/150.45 ± 17.35mmHg is than the low 18.61/16.98mmHg of model group 228.64 ± 21.91/167.43 ± 19.65mmHg (p＜0.01) in the Herba Dendrobii.Treatment is during 12 weeks, and dosage group blood pressure 216.24 ± 21.14/156.47 ± 18.75mmHg in the Herba Dendrobii is than the low 21.76/18.46mmHg (p＜0.05) of model group 238.00 ± 28.84/174.93 ± 24.48mmHg; The 1st all hypertension when 12 week treatment back drug withdrawals of the large, medium and small dosage group of Herba Dendrobii are not obvious, hypertension 39.55/27.44mmHg when the drug withdrawal 1 after the treatment of 12 weeks of Western medicine nimodipine matched group is all, and the hypertension amplitude is bigger; 1-9 week treatment is respectively organized blood pressure and is all maintained the level lower than model group after the drug withdrawal, 4 whens week after the drug withdrawal, dosage group blood pressure 214.94 ± 15.68/157.22 ± 14.02mmHg in the Herba Dendrobii is than the low 36.11/30.00mmHg of model group blood pressure 251.05 ± 13.23/187.22 ± 13.10mmHg; In 10-36 week after the drug withdrawal, treatment is respectively organized blood pressure and is also all maintained the level lower than model group.In 36 whens week after the drug withdrawal, dosage group blood pressure 248.78 ± 16.02/180.95 ± 12.70mmHg in the Herba Dendrobii is than the low 23.68/25.01mmHg of model group blood pressure 272.46 ± 2.83/205.96 ± 6.70mmHg.The large, medium and small dosage group of 1-36 week Herba Dendrobii is minimum with middle dosage group and heavy dose of group blood pressure after the drug withdrawal.
2. existence natural law:
The large, medium and small dosage group of Herba Dendrobii is treated the SHR-sp rat all the effect of certain prolongation existence natural law 12 weeks.Treat and 8 weeks began to have certain effect, treat after 12 weeks comparatively remarkable, wherein best with the effect of middle dosage group; The large, medium and small dosage group of 1-36 week Herba Dendrobii is better with the effect of big or middle dosage group after the drug withdrawal.Wherein, healthy existence of 36 weeks natural law are 243.21 ± 122.71 days after the drug withdrawal of middle dosage group, be significantly higher than model group 103.94 ± 100.61 days (p＜0.001) and small dose group 156.58 ± 120.20 days (p＜0.05).Dosage group 272.08 ± 147.23 days in the 45 whens week average healthy existence natural law Herba Dendrobii is model group 111.14 ± 118.22 days 2.45 times (p＜0.001) after the drug withdrawal.Best in each treatment group with dosage group prolongation existence natural law effect in the Herba Dendrobii.The large, medium and small dosage group of Herba Dendrobii prolongs existence natural law effect after 12 week treatment drug withdrawals more remarkable in than 12 all therapeutic processes.
3. survival rate (survival ratio):
The large, medium and small dosage group of Herba Dendrobii is treated the survival rate effect that all improved in 12 weeks to the SHR-sp rat oral gavage.Treat and 10 weeks began to have remarkable effect, treat after 12 weeks more remarkable.Treatment is better with the effect of big or middle dosage group raising survival rate in the large, medium and small dosage group of Herba Dendrobii during 12 weeks, is respectively 23/32 (71.88%), 19/24 (79.17%), is significantly higher than model group 13/35 (37.14%) (p＜0.01, p＜0.001); After the drug withdrawal in the large, medium and small dosage group of 1-45 week Herba Dendrobii big or middle dosage group survival rate be significantly higher than small dose group.In 14-45 week after the drug withdrawal, Western medicine group and model group be there was no significant difference relatively.Wherein, after the drug withdrawal 9-12 in week in the Herba Dendrobii survival rate of dosage group be 17/24 (70.83%), highly significant is higher than 5/35 (14.29%) (p＜0.001) of 9-12 model group in week after the drug withdrawal.45 weeks after the drug withdrawal, dosage group survival rate 11/24 (45.83%) in the Herba Dendrobii, be significantly higher than model group survival rate 4/35 (11.43%) (p＜0.01, p＜0.05), Western medicine nimodipine group survival rate 7/22 (31.82%) does not have significant difference (p＞0.05) with model group; The dosage group is treated the ratio that the survival rate of 36-45 during week after 32-34 week after 13-28 week after 9-12 week after 4-5 week after 12 weeks, the drug withdrawal, the drug withdrawal, the drug withdrawal, the drug withdrawal, the drug withdrawal be significantly higher than the model group survival rate and is reached 2.13 times (79.17%/37.14%), 3.08 times (79.17%/25.71%), 4.96 times (70.83%/14.29%), 4.67 times (66.67%/14.29%), 4.37 times (50.00%/11.43%), 4.01 times (45.83%/11.43%) respectively in the Herba Dendrobii.Best in each treatment group with dosage group raising survival rate effect in the Herba Dendrobii.
4. heart and brain kidney weight/weight ratio:
Dosage group in the Herba Dendrobii, heart/weight ratio, the brain/weight ratio of animal had the trend less than model group when the experiment of Western medicine nimodipine group finished.When experiment finishes, the brain/weight ratio 0.00481 ± 0.00058 of dosage group in the Herba Dendrobii, remarkable brain/weight ratio 0.00772 ± 0.00253 (p＜0.05) less than the model group animal.
Three, zooperal conclusion:
Dendrobium officinale aqueous extract has the persistent effect of bringing high blood pressure down of mitigation to the SHR-sp rat; Dendrobium officinale aqueous extract has the effect that prolongs life span, improves survival rate, prevention of stroke to the SHR-sp rat; Dendrobium officinale aqueous extract has certain dependency to bring high blood pressure down, prolong life span, raising survival rate, prevention of stroke effect and the consumption of SHR-sp rat.
Four, the preparation of Herba Dendrobii related drugs:
Dendrobium officinale aqueous extract is when the medicine of preparation prophylactic treatment hypertension and hypertension apoplexy, can adopt the generally accepted carrier formats of pharmacy such as extractum, oral liquid, granule, capsule or tablet: extractum, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation extractum are used, wherein the mass content of active component dendrobium officinale aqueous extract is 8～80%; Oral liquid is made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation extractum are used, and wherein the mass content of active component dendrobium officinale aqueous extract is 0.1～16%; Granule is made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation extractum are used, and wherein the mass content of active component dendrobium officinale aqueous extract is 2～80%; Capsule is made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation capsule are used, and wherein the mass content of active component dendrobium officinale aqueous extract is 10～50%; Tablet is made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation tablet are used, and wherein the mass content of active component dendrobium officinale aqueous extract is 10～50%.
The said medicine preparation method is as follows:
Extract making method may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) the water logging mixture with Herba Dendrobii places the extraction pot heating extraction 2-3 time, each 1-8 hour, filters;
(3) extracting solution is concentrated into relative density 1.000～1.150,300,000 grades of clean area fills;
(4) semi-finished product are put the sterilization of sterilization cabinet, promptly.
The preparation method of oral liquid may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix adjuvants such as pharmaceutically acceptable flavoring agent, antiseptic, packing, sterilization, promptly.
The preparation method of granule may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories or medicated powder such as glucose, sucrose, starch, cellulose, spray granulation, packing, promptly.
Capsular preparation method may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories or medicated powder, spray granulation divides the capsule of packing into, and irradiation-sterilize promptly.
The preparation method of tablet may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) the extracting solution concentrated solution mixes acceptable accessories or medicated powder such as glucose, sucrose, starch, cellulose, spray granulation, and tabletting, packing, promptly.
Five, the instructions of taking of Herba Dendrobii related drugs:
Because easily the pathological characters similarity degree of the pathological characters of apoplectic type spontaneous hypertensive rat (SHR-sp) and human hypertension, apoplexy is higher, so this animal model is usually used in the pharmacodynamic study of human body hypertension and hypertension apoplexy.According to aforementioned zooperal Herba Dendrobii effective dose data, and the general animal of applied pharmacology-human drug dose conversion method can learn that people's clinical effective dose is 2～8 gram Herba Dendrobii crude drug/skies.
According to this human dosage standard:
The mass content of active component dendrobium officinale aqueous extract is 8.0%～80% in extractum, and the dose of human extractum is every day 2～3 times, and each 0.5～8 gram extractum amount is comparatively suitable;
The mass content of active component dendrobium officinale aqueous extract is 2%～80% in granule, and the dose of people's medication is every day 2～3 times, and each 1～5 bag amount is comparatively suitable; [notes granule: 2～10 gram/bags]
The mass content of active component dendrobium officinale aqueous extract is 10%～50% in capsule, and the dose of people's medication is every day 2～3 times, and each 1～16 amount is comparatively suitable; [injecting glue capsule: 0.2～0.5 gram/grain]
The mass content of active component dendrobium officinale aqueous extract is 10%～50% in tablet, and the dose of people's medication is every day 2～3 times, and each 1～8 amount is comparatively suitable; [notes tablet: 0.1～1.0 gram/sheet]
The mass content of active component dendrobium officinale aqueous extract is 0.1%～16% in oral liquid, and the dose of people's medication is every day 2～3 times, and each 5～200ml amount is comparatively suitable.
At last, it is also to be noted that what more than enumerate only is specific embodiments of the invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (11)
1. the application of dendrobium officinale aqueous extract in the medicine of preparation prevention, treatment hypertension or apoplexy.
2. the application of dendrobium officinale aqueous extract according to claim 1 in the medicine of preparation prevention, treatment hypertension or apoplexy, it is characterized in that, described medicine is a Herba Dendrobii extractum, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation extractum are used, wherein the mass content of active component dendrobium officinale aqueous extract is 8.0%～80%.
3. the application of dendrobium officinale aqueous extract according to claim 2 in the medicine of preparation prevention, treatment hypertension or apoplexy is characterized in that the preparation of described Herba Dendrobii extractum may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into 0.1～2.0 gram crude drug/gram, perhaps extracting solution is concentrated into relative density 1.000～1.150, in acceptable accessories refined honey, maltose, sucrose or the Mel that adding preparation extractum or taste cream are used one or more, the mass content that makes the active component dendrobium officinale aqueous extract is 8.0%～80%, mix homogeneously is 300,000 grades of clean area fills;
(4) semi-finished product are put to sterilize in the sterilization cabinet and are promptly got Herba Dendrobii extractum finished product.
4. the application of dendrobium officinale aqueous extract according to claim 1 in the medicine of preparation prevention, treatment hypertension or apoplexy, it is characterized in that, described medicine is the Herba Dendrobii granule, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation granule are used, wherein the mass content of active component dendrobium officinale aqueous extract is 2.0%～80%.
5. the application of dendrobium officinale aqueous extract according to claim 4 in the medicine of preparation prevention, treatment hypertension or apoplexy is characterized in that the preparation of described Herba Dendrobii granule may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) the extraction concentrated solution mixes one or more in acceptable accessories glucose, sucrose, starch, the cellulose, the mass content that makes the active component dendrobium officinale aqueous extract is 2.0%～80%, spray granulation, packing promptly gets Herba Dendrobii granule finished product.
6. the application of dendrobium officinale aqueous extract according to claim 1 in the medicine of preparation prevention, treatment hypertension or apoplexy, it is characterized in that, described medicine is the Herba Dendrobii capsule, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation capsule are used, wherein the mass content of active component dendrobium officinale aqueous extract is 10%～50%.
7. the application of dendrobium officinale aqueous extract according to claim 6 in the medicine of preparation prevention, treatment hypertension or apoplexy is characterized in that the capsular preparation of described Herba Dendrobii may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories or medicated powder to make the mass content of active component dendrobium officinale aqueous extract be 10%～50%, spray granulation divides the capsule of packing into, and irradiation-sterilize promptly gets the Herba Dendrobii capsule finished product.
8. the application of dendrobium officinale aqueous extract according to claim 1 in the medicine of preparation prevention, treatment hypertension or apoplexy, it is characterized in that, described medicine is the Herba Dendrobii tablet, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation tablet are used, wherein the mass content of active component dendrobium officinale aqueous extract is 10%～50%.
9. the application of dendrobium officinale aqueous extract according to claim 8 in the medicine of preparation prevention, treatment hypertension or apoplexy is characterized in that the preparation of described Herba Dendrobii tablet may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) the extracting solution concentrated solution mixes one or more in acceptable accessories glucose, sucrose, starch or the cellulose, the mass content that makes the active component dendrobium officinale aqueous extract is 10%～50%, spray granulation, tabletting, packing promptly gets Herba Dendrobii tablet finished product.
10. the application of dendrobium officinale aqueous extract according to claim 1 in the medicine of preparation prevention, treatment hypertension or apoplexy, it is characterized in that, described medicine is the Herba Dendrobii oral liquid, be made up of the adjuvant that active component dendrobium officinale aqueous extract and pharmaceutically acceptable preparation oral liquid are used, wherein the mass content of active component dendrobium officinale aqueous extract is 0.1%～16%.
11. the application of dendrobium officinale aqueous extract according to claim 10 in the medicine of preparation prevention, treatment hypertension or apoplexy is characterized in that the preparation of described Herba Dendrobii oral liquid may further comprise the steps:
(1), adds the water logging bubble of 10～30 times of weight with the stem draw down of Herba Dendrobii, broken section one-tenth coarse fodder;
(2) above-mentioned Herba Dendrobii water logging mixture is placed the extraction pot heating extraction 2～3 times, each 1～8 hour, filter;
(3) extracting solution is concentrated into relative density 1.000～1.150;
(4) extract concentrated solution and mix acceptable accessories flavoring agent or antiseptic to make the mass content of active component dendrobium officinale aqueous extract be 0.1%～16%, packing, sterilization promptly gets Herba Dendrobii oral liquid finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101011375A CN101612316B (en) | 2009-07-23 | 2009-07-23 | Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101011375A CN101612316B (en) | 2009-07-23 | 2009-07-23 | Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101612316A CN101612316A (en) | 2009-12-30 |
| CN101612316B true CN101612316B (en) | 2011-06-22 |
Family
ID=41492325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101011375A Active CN101612316B (en) | 2009-07-23 | 2009-07-23 | Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101612316B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843851B (en) * | 2010-06-08 | 2011-11-09 | 浙江天皇药业有限公司 | Dendrobium officinale nimodipine combination medicine for resisting hypertension and apoplexia, and preparation method thereof |
| CN101849957B (en) * | 2010-06-08 | 2012-04-18 | 浙江天皇药业有限公司 | Application of Dendrobium officinale polysaccharide in preparation of medicine for preventing and treating hypertension and apoplexy |
| CN104544038A (en) * | 2013-10-29 | 2015-04-29 | 广西浙商投资有限公司 | Dendrobium officinale oral liquid preparation and processing method |
| CN105166912B (en) * | 2015-10-22 | 2018-06-22 | 李佳忠 | A kind of compound Moringa stem of noble dendrobium oral liquid and preparation method thereof |
| CN107260963B (en) * | 2017-06-15 | 2020-12-04 | 杭州丹鹤医药有限公司 | Method for preparing medicine for treating hypertension by using dendrobium officinale and radish seed as main materials |
| CN107281386B (en) * | 2017-06-15 | 2020-10-16 | 杭州丹鹤医药有限公司 | Method for preparing medicine for treating hypertension by using dendrobium officinale and radish seeds and application |
| CN107714949A (en) * | 2017-09-20 | 2018-02-23 | 浙江省中医药研究院 | Dendrobium candidum is preparing the application process in treating hypertension drug |
| CN111603540A (en) * | 2020-06-11 | 2020-09-01 | 南京希元生物医药科技有限公司 | Preparation method of dendrobium extract |
-
2009
- 2009-07-23 CN CN2009101011375A patent/CN101612316B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 鲍丽娟,等.4种石斛水提物对人宫颈癌HelaS3细胞和肝癌HepG2细胞的抑制作用.《安徽农业科学》.2008,第36卷(第36期),15968-15970. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101612316A (en) | 2009-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101612316B (en) | Application of dendrobium officinale aqueous extract in preparing medicine for preventing and treating hypertension and apoplexy | |
| CN101849957B (en) | Application of Dendrobium officinale polysaccharide in preparation of medicine for preventing and treating hypertension and apoplexy | |
| CN101411760B (en) | Novel use of plants in sorbus malus group of malus | |
| WO2012027882A1 (en) | Pharmaceutical composition for treating insomnia and preparation method thereof | |
| CN102102079B (en) | Blood pressure-reducing table vinegar and preparation method thereof | |
| CN104522639A (en) | Anti-intoxication, alcohol-expelling and liver-protection preparation and preparation process thereof | |
| CN107343902A (en) | It is a kind of that there is herbal composite for alleviating hypothyroidism effect and preparation method and application | |
| CN102293936B (en) | Medicinal composition or health food composition for protecting gastric mucosa, and preparation method thereof | |
| CN101856466B (en) | Medicine preventing and treating hypertension and stroke and being prepared by dendrobium officinale flower and method | |
| CN101961366B (en) | Preparation method of malan straw extract, malan straw product, pharmaceutical composition and application thereof | |
| CN1558768A (en) | A pharmaceutical composition made from Chinese traditional medicine and preparation method thereof | |
| CN103142916B (en) | Medicament for preventing and treating senile dementia and preparation method thereof | |
| CN102940800A (en) | Traditional Chinese medicine for treating diabetes | |
| CN103446266A (en) | Traditional Chinese medicinal compound preparation for treating type 2 diabetes, and preparation method thereof | |
| CN102641357A (en) | Medicament for treating hypertension and preparation method thereof | |
| CN104666931B (en) | Composition and its application with liver-protecting and alcoholism-relieving effect | |
| CN104042928B (en) | A kind of pharmaceutical composition for treating diabetes and its production and use | |
| CN101843851B (en) | Dendrobium officinale nimodipine combination medicine for resisting hypertension and apoplexia, and preparation method thereof | |
| CN102327304B (en) | Drug for preventing and controlling chronic mountain sickness and preparation method thereof | |
| CN101683461B (en) | Application of a traditional Chinese medicine composition in preparation of medicament for inhibiting oxidation damage | |
| CN101716200B (en) | Medicinal composition for invigorating circulation blood and dredging collaterals and clearing heat and releasing toxin and preparation method and application thereof | |
| CN103083423A (en) | Traditional Chinese prescription for treating hyperglycemia and preparation method thereof | |
| CN102552481A (en) | Traditional Chinese medicinal composition with effects of smoothing collaterals and lowering blood pressure and preparation method thereof | |
| CN103230552A (en) | Traditional Chinese medicine for treating diabetes mellitus | |
| CN108478701A (en) | A kind of lozenge of strengthen immunity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |