CN108371241A

CN108371241A - A kind of feed and preparation method thereof improving camel mammary gland function

Info

Publication number: CN108371241A
Application number: CN201810141446.4A
Authority: CN
Inventors: 隆杰
Original assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Current assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Priority date: 2018-02-11
Filing date: 2018-02-11
Publication date: 2018-08-07

Abstract

The present invention relates to feed processing technology fields, more particularly to a kind of feed and preparation method thereof improving camel mammary gland function, the feed of the application is made of additive, fermentation mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, Bai Ci and sand sagebrush (Artemisia filifolia), wherein, additive component is the active ingredient that camel mammary gland function can be improved of the application applicant's independent research；The small-molecule substances such as amino acid therein, vitamin are adequately released after the mulberry leaf of the application, Folium Pterocaryae, pine needle fermentation, are suitble to the female camel constitution just produced；And also contain abundant flavone component energy effective protection camel ovary in pine needle, promote the secretion of camel estrogen；Thorn and sand sagebrush (Artemisia filifolia) contain abundant crude protein in vain in feed, basic nutrition material requisite of female camel to nonirrigated farmland herbage crude protein can be met, female camel can be met to the primary demand of nutriment, the application feed formula is reasonable, can safely and effectively improve camel mammary gland function.

Description

A kind of feed and preparation method thereof improving camel mammary gland function

【Technical field】

The present invention relates to feed processing technology field, more particularly to a kind of feed improving camel mammary gland function and its preparation Method.

【Background technology】

The various nutritional ingredients that bactrian camel milk and camel dairy products are needed containing human body are best one of the wholefoods of the mankind, Bactrian camel milk is developed and used to improving household's actions, grows local economy, development characteristic dairy products industry, meeting the market demand has Significance.Since reducing the process and development with camel milk due to domestic camel cultivation quantity, cause the price of camel also continuous It is soaring, to bring new vitality to the development of cultivation camel industry.But it is current camel milk development that the camel output of milk is not high Bottleneck problem in the process.

The output of milk of camel depends primarily on the supply situation of feed and water, improves nutritive value in feed and is produced to improving Milk amount plays a very important role.It in camel breeding process, mostly uses to put in a suitable place to breed camel is cultivated, this is because camel It is higher to the taste requirements of feed, if feed will not be eaten by being unable to effective stimulus camel taste bud camel, during conventional cultivation, Camel to the selection of forage grass be with seasonal variations, and it is selective during feeding, especially like seasonal forage. And the more annual herbage of preference feeding；Since " particular about food " of camel is currently, there are no the researchs to its formula forage, and match Square feed, which has, to be convenient for carrying, feeds advantage easy to operate.And during female camel is put in a suitable place to breed, since camel growing environment is disliked It is bad, it is mostly grown in desert, female camel is easy to that mammary gland blockage, ovary can be led to because the feed formula of intake is unreasonable The problems such as function reduction, these problems will influence camel lactation；For this purpose, how to develop it is a kind of safely, effectively, meet white horse with a black mane The formula forage of hunchbacked mouthfeel, simultaneously, moreover it is possible to improve camel mammary gland function, the lactation function of further increasing camel is camel cultivation Personnel need urgently to solve the problems, such as.

【Invention content】

In view of the above, it is necessary to provide a kind of camel formula forage, camel mammary gland function can be improved, while also pacifying Entirely, effectively, meet camel taste demand.

In order to achieve the above objectives, the technical solution adopted in the present invention is：

A kind of feed improving camel mammary gland function, the feed comprise the following components in parts by weight：8 parts -14 parts Additive, 41 parts -58 parts of fermentation mulberry leaf, 27 parts -37 parts of fermentation Folium Pterocaryae, 21 parts -41 parts of fermentation pine needle, 19 parts - 37 parts of white thorn and 20 parts -36 parts of sand sagebrush (Artemisia filifolia).

Further, the additive in the application feed comprises the following components in parts by weight：65 parts -76 parts of fat Acid, 23 parts -34 parts of mango core essential oil, 18 parts -29 parts of glossy privet fruit extract, 23 parts -31 parts of Semen sojae atricolor extract, 21 parts - 33 parts of Thallus Laminariae (Thallus Eckloniae) extract and 23 parts -35 parts of analysis for soybean powder.

Further, present invention also provides the preparation method of above-mentioned aliphatic acid, described method includes following steps：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 Part -23 parts of stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk are pre-processed respectively, then into Row mixing obtains extraction material；

(2) by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v) according to mass ratio It is 1:1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, is directly put after taking-up Enter in the water bath with thermostatic control that temperature is 90 DEG C -100 DEG C to thaw, heating, when the temperature of mixture reaches bath temperature, continue Heating water bath 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasound carries The ultrasonic power density taken is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；It is ultrasonically treated according to " opening is super It is that 1 processing mode recycled carries out that sound, which be ultrasonically treated 15s- and close ultrasound 5s- and open ultrasound to carry out supersound process 15s ", Reprocessing carries out 20-30 circular treatment in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in thermostatted water under 50 DEG C -60 DEG C of temperature condition 30min-40min is bathed, it is 1 to be cooled to after room temperature according to volume ratio:1 is added the item that petroleum ether-ether is 50 DEG C -70 DEG C in temperature Extraction 60min-70min is carried out under part, and extract I is obtained after taking ether layer recycling design；It is according to solid-liquid mass ratio into filter residue 1:The petroleum ether that percentage by volume is 10% (v/v) -20% (v/v) is added in 8-10, is placed in the condition that temperature is 65 DEG C -75 DEG C Lower constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed The aliphatic acid is obtained after conjunction.

Further, the preprocess method of step (1) peanut is in addictive preparation method：By after peanut decladding with quality The NaHCO of a concentration of 1%-3%₃Solution is 2-4 according to solid-liquid mass ratio:1-2 is mixed, and drying is to moisture content later for grinding 3%-5% to obtain the final product.

Further, the preprocess method of step (1) oil tea slag is in addictive preparation method：Oil tea slag and quality is dense The NaCl solution that degree is 1%-3% is 2-4 according to solid-liquid mass ratio:1 is mixed, water-bath 2h- under conditions of 60 DEG C -70 DEG C 3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) stool in mice is in addictive preparation method：It is by mouse moisture content The stool in mice of 2%-4% is pulverized, and is then 5.5 × 10 with living bacteria count⁹~8.1 × 10⁹CFU/ml bacillus subtilis bacterium Liquid is 20-30 according to mass ratio:1 is mixed, anaerobic fermentation 15d-20d under conditions of 25-27 DEG C, produces fermentation after fermentation The NaCl solution that object is 1%-3% with mass concentration is 3-5 according to mass ratio:1 is mixed, under conditions of 65 DEG C -75 DEG C Water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) suspended sludge in milk wastewater is in addictive preparation method：By Dairy Wastewater It is 2.5 × 10 to be filtered and take filter residue and living bacteria count⁹~6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 15- according to mass ratio 20:1 is mixed, and tunning and mass concentration are by anaerobic fermentation 20d-25d under conditions of 23-25 DEG C after fermentation The NaHCO of 1%-3%₃Solution is 3-5 according to mass ratio:1-2 is mixed, water-bath 2h-3h mistakes under conditions of 45 DEG C -55 DEG C Leaching filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) milk is in addictive preparation method：By milk and living bacteria count It is 3.5 × 10⁹~6.7 × 10⁹CFU/ml saccharomycete bacterium solution is 18-23 according to mass ratio:1 is mixed, in 26-28 DEG C of item Ferment 10d-15d under part, by NaHCO that tunning and mass concentration are 1%-3% after fermentation₃Solution is 2- according to mass ratio 6:2-3 is mixed, and water-bath 1h-2h under conditions of 45 DEG C -55 DEG C to obtain the final product.

Further, the preparation method of the application feed further includes following steps：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 30-35 according to mulberry leaf and probiotics mass ratio: 1 addition probiotics I ingredients are 22 DEG C -28 DEG C in temperature, and humidity is fermented under conditions of being 17%-27%, and fermentation time is 18d-23d, drying is that 15%-25% obtains the fermentation mulberry leaf to moisture content under the conditions of 55 DEG C -60 DEG C；The probiotics I Ingredient by the living bacteria count after activating be 3.5 × 10¹⁰~8.7 × 10¹⁰CFU/g saccharomycete and living bacteria count be 3.1 × 10¹⁰~9.3 × 10¹⁰CFU/g lactic acid bacterias are 1-4 according to mass ratio:1-3 is formed；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 5-10 by the leaf of fresh weeping willow and shoot:1 is mixed Conjunction shreds, and is then 33-43 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 22 DEG C -28 in temperature DEG C, humidity is fermented under conditions of being 15%-25%, and fermentation time 20d-30d is dried under the conditions of 55 DEG C -60 DEG C and arrived Moisture content is that 10%-20% obtains the fermentation Folium Pterocaryae；The ingredient of the probiotics II is by the living bacteria count after activating 2.5×10¹⁰~9.2 × 10¹⁰CFU/g yeast is mould and living bacteria count is 2.9 × 10¹⁰~9.2 × 10¹⁰CFU/g bacillus subtilis Bacterium is 2-4 according to mass ratio:1-3 is formed；

(3) pine needle prepares fermentation pine needle：It is 30-35 by pine needle and probiotics mass ratio:1 addition probiotics III at Point, it is 26 DEG C -37 DEG C in temperature, humidity is fermented under conditions of being 18%-22%, fermentation time 20d-33d, 60 Drying obtains the fermentation pine needle to moisture content for 15%-20% under the conditions of DEG C -65 DEG C；The ingredient of the probiotics III is by living Living bacteria count after change is 1.6 × 10¹⁰~8.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 1.3 × 10¹⁰ ~9.5 × 10¹⁰CFU/g aspergillus oryzaes are 2-5 according to mass ratio:1-3 is formed；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 5%-15% are 7%-12% crushes after being thoroughly mixed, by 30 - 100 mesh screen of mesh screens, and finally adds additive, and drying to moisture content is that 3%-5% obtains the feed.

The present invention has the advantages that：

1, the feed of the application is made of additive, fermentation mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, Bai Ci and sand sagebrush (Artemisia filifolia), Wherein, additive component is the active ingredient that camel mammary gland function can be improved of the application applicant's independent research；Mulberry leaf, willow Contain abundant protein in leaf, pine needle, mulberry leaf, Folium Pterocaryae, pine needle smell faint scent are pleasant, while mouthfeel is soft, pollution It is few, while also containing abundant flavone component energy effective protection camel ovary in pine needle, promote the secretion of camel estrogen, these Herbage has strong ferment fragrance that can increase feed palatability after everfermentation；And mulberry leaf, Folium Pterocaryae, pine needle fermentation in feed The small-molecule substances such as amino acid therein, vitamin are adequately released afterwards, are suitble to the female camel constitution just produced；This Shen Also containing certain herbaceous plants with big flowers in feed please, thorn and sand sagebrush (Artemisia filifolia), these ingredients contain abundant crude protein in vain, can meet female camel to nonirrigated farmland herbage The basic nutrition material requisite of crude protein, feed can be applied more easily after rational formula, processing and be cultivated with camel.

2, used probiotics all have passed through activation to the mulberry leaf, Folium Pterocaryae, pine needle of the application during the fermentation, activation Different degrees of in culture medium to add white mulberry vein-juice, willow leaf juice, pine needle juice etc., these ingredients can be probiotic enriched, accelerates benefit Fermentation of the raw bacterium to mulberry leaf, Folium Pterocaryae, pine needle.

3, the feed addictive of the application is by aliphatic acid, mango core essential oil, glossy privet fruit extract, Semen sojae atricolor extract, kelp Extract and analysis for soybean powder form, and in ingredient, aliphatic acid is the aliphatic acid that applicant independently extracts, wherein containing not compared with horn of plenty Saturated fatty acid, unsaturated fatty acid Linoleic acid content 69.47% or so, 22.02% or so take in by oleic acid content Amount is more will not to lead to that camel cholesterol is excessively high, the fat effect for blocking mammary gland of camel；Contain abundant oil in mango core essential oil The plurality of active ingredients such as acid, palmitic acid and Octadec-9-enoic Acid contain abundant oleic acid, leukotrienes, sweet dew in the fruit of glossy privet Alcohol, these ingredients all have the function of removing biological interior free yl, inhibit bacterium, saccharomycete and fungi growth, can effectively rise To antioxidation, abundant protein, general flavone are contained in Semen sojae atricolor extract；Contain abundant thick egg in Thallus Laminariae (Thallus Eckloniae) extract In vain, core yellow cellulose content, vitamin B1；The ovary work(of animal can be protected in analysis for soybean powder containing abundant lecithin these ingredients Can, promote ovary effectively to secrete estrogen so as to balance camel hormone in vivo content, allow camel mammogenesis faster；So as to The purpose of protection camel mammary gland function is played in interaction in terms of dredging mammary gland pipeline, promoting hormonal balance two.

【Specific implementation mode】

All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.

Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each Feature is an example in a series of equivalent or similar characteristics.

Embodiment 1：

A kind of feed improving camel mammary gland function is present embodiments provided, the feed is by following parts by weight at grouping At：8 parts of additive, 41 parts fermentation mulberry leaf, 27 parts fermentation Folium Pterocaryae, 21 parts fermentation pine needle, 19 parts it is white pierce and 20 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of Thallus Laminariae (Thallus Eckloniae) extract and 23 parts of analysis for soybean powder.

The extracting method of above-mentioned glossy privet fruit extract is：The fruit of glossy privet seed that moisture content is 10% is pulverized and volume basis The butanol that number is 10% is according to solid-liquid mass ratio 1:1 is mixed, temperature be 70 DEG C stirred in water bath temperature soak 3 times, every time 40min, filtering, merging filtrate carries out vacuum distillation concentration until paste, the relative density of paste is 1.66g/cm³Obtain institute Glossy privet fruit extract is stated, extract mid-oleic is 41.65%；Linolenic acid content is 34.87%；Mannitol content is 11.21%.

The extracting method of above-mentioned Semen sojae atricolor extract is：After black soya bean is soaked with water according to mass ratio be 1:1 adds water to be ground Mill, is then placed in 90 DEG C of water-baths and preheats, and it is 5% then to add with the mass percent of mixed liquor equal quality ratio NaHCO₃Solution is then placed in constant temperature blender with magnetic force and shakes, and protein is made to be dissolved in 3% NaHCO₃Solution, 1000r/min 30min is centrifuged, supernatant is collected, obtains albumin；Take the sodium hydroxide solution that centrifugation lower layer's substance is 3% with mass percent Then mixing collects supernatant according to same method (centrifugal rotational speed, time are identical), obtains globulin；Residue is added again The ethanol solution that percentage by volume is 75%, dissolving collect supernatant, obtain protein,alcohol-soluble；Then by above-mentioned albumin, ball It is 10% that albumen and protein,alcohol-soluble, which mix and dry moisture content, obtains Semen sojae atricolor extract, protein contains in Semen sojae atricolor extract Amount is 754.25mg/g；General flavone content is 216.25mg/g.

The extracting method of above-mentioned Thallus Laminariae (Thallus Eckloniae) extract is：Fresh Laminaria Japonica is shredded, with organic solvent mixed liquor according to mass ratio It is 1:2 are mixed, and organic solvent mixed liquor is 30% ethyl acetate and 65% ethyl alcohol according to quality by percentage by volume Than being 1:1 is mixed, and refluxing extraction is then carried out in refluxing extraction device, and Extracting temperature is 105 DEG C, extraction time 2h, It filters to take filtrate and carries out vacuum distillation concentration until paste, the relative density of paste is 1.49g/cm³, obtain the kelp and carry Take object, crude protein content is 241.04mg/g in extract, core yellow cellulose content is 96.24mg/g, vitamin B1 content is 25.07mg/g。

The lecithin content of above-mentioned analysis for soybean powder is 31.11%.

The preparation method of aliphatic acid includes the following steps in above-mentioned additive：

(1) it is by parts by weight：25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts of stool in mice, 9 parts Suspended sludge in milk wastewater and 9 parts of milk pre-processed respectively, then mixed, obtain extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -20 DEG C and suddenly freezes 30min, water bath with thermostatic control at a temperature of 90 °C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 10min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 400w/L；It is ultrasonically treated Temperature is 130 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 20 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1:The HCl solution that 1 addition mass concentration is 2% is placed in water bath with thermostatic control 30min under 50 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 60min- under conditions of temperature is 50 DEG C 70min obtains extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:8, which are added percentage by volumes, is The petroleum ether of 10% (v/v) is placed in constant temperature under conditions of temperature is 65 DEG C and extracts 70min, obtained after taking ether layer recycling design Extract II；Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of step (1) peanut is in the present embodiment addictive preparation method：By after peanut decladding with quality A concentration of 1% NaHCO₃Solution is 2 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content be 3% to obtain the final product.

The preprocess method of step (1) oil tea slag is in the present embodiment addictive preparation method：Oil tea slag and quality is dense The NaCl solution that degree is 1% is 2 according to solid-liquid mass ratio:1 is mixed, and water-bath 2h filters to take filter residue baking under conditions of 60 DEG C It is 3% to do moisture content to obtain the final product.

The preprocess method of step (1) stool in mice is in the present embodiment addictive preparation method：It is by mouse moisture content 2% stool in mice is pulverized, and is then 5.5 × 10 with living bacteria count⁹CFU/ml bacillus subtilis bacterium solutions are according to mass ratio It is 20:1 is mixed, anaerobic fermentation 15d under conditions of 25 DEG C, by tunning and mass concentration is 1% after fermentation NaCl solution is 3 according to mass ratio:1 is mixed, and water-bath 2h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C is 3% to obtain the final product.

The preprocess method of step (1) suspended sludge in milk wastewater is in the present embodiment addictive preparation method：By Dairy Wastewater It is 2.5 × 10 to be filtered and take filter residue and living bacteria count⁹CFU/ml lactic acid bacterial liquids are 15 according to mass ratio:1 is mixed, Anaerobic fermentation 20d under conditions of 23 DEG C, the NaHCO for being 1% by tunning and mass concentration after fermentation₃Solution is according to quality Than being 3:1 is mixed, under conditions of 45 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3% to obtain the final product.

The preprocess method of step (1) milk is in the present embodiment addictive preparation method：By milk and living bacteria count It is 3.5 × 10⁹CFU/ml saccharomycete bacterium solution is 18 according to mass ratio:1 is mixed, and ferment 10d under conditions of 26 DEG C, fermentation The NaHCO for being afterwards 1% by tunning and mass concentration₃Solution is 2 according to mass ratio:2 are mixed, in 45 DEG C DEG C of condition Lower water-bath 1h to obtain the final product.

Wherein, the addictive preparation method of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:20 be sufficiently mixed after in 1000r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added into the emulsion of step (2), The vaseline that mixture quality 1/5 is added after being sufficiently stirred, it is 2.31g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The present embodiment feed producing method is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 30 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, is 22 DEG C in temperature, humidity is fermented under conditions of being 17%, fermentation time 18d, in 55 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 15% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 3.5 × 10¹⁰CFU/g saccharomycete and living bacteria count are 3.1 × 10¹⁰CFU/g lactic acid bacterias are 1 according to mass ratio:1 composition；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 5 by the leaf of fresh weeping willow and shoot:1 mixing is cut It is broken, it is then 33 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 22 DEG C in temperature, humidity 15% Under conditions of ferment, fermentation time 20d, under the conditions of 55 DEG C drying obtain the fermentation willow to moisture content for 10% Leaf；The ingredient of the probiotics II is 2.5 × 10 by the living bacteria count after activating¹⁰CFU/g yeast is mould and living bacteria count is 2.9×10¹⁰CFU/g bacillus subtilises are 2 according to mass ratio:1 composition；

(3) pine needle prepares fermentation pine needle：It is 30 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 26 DEG C in temperature, humidity is fermented under conditions of being 18%, and fermentation time 20d is dried under the conditions of 60 DEG C to aqueous Rate obtains the fermentation pine needle for 15%；The ingredient of the probiotics III by the living bacteria count after activating be 1.6 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 1.3 × 10¹⁰CFU/g aspergillus oryzaes are 2 according to mass ratio:1 composition；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 5% are 7% crushes after being thoroughly mixed, and is screened by 30 mesh screens, Additive is finally added, drying to moisture content obtains the feed for 3%.

The formula of the present embodiment probiotics I activation mediums is (total volume 1L)：Mass concentration is the fiber of 20mg/mL Element, mass concentration be 5mg/mL glucose, mass concentration be 21mg/mL beef extract, mass concentration be 15mg/mL albumen Seawood meal that agar that peptone, mass concentration are 10mg/mL, mass concentration are 8mg/mL, remaining be white mulberry vein-juice；Wherein, white mulberry vein-juice Processing method be：According to mass ratio it is 20 by cellulase and water that mulberry leaf, enzyme activity intensity are 500U/g:1:80 are mixed It closes, is put into 37 DEG C of insulating boxs and reacts 3d, filtering removal filtrate is made.

The formula of II activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 20mg/mL Tie up the beef extract that element, the aloe polysaccharide that mass concentration is 10mg/mL, mass concentration are 25mg/mL, mass concentration 15mg/mL Yeast extract, mass concentration be 9mg/mL seawood meal, mass concentration be 15mg/mL agar, remaining be willow leaf juice；Its In, the processing method of willow leaf juice is：It is according to mass ratio by cellulase and water that Folium Pterocaryae, enzyme activity intensity are 500U/g 30:1:90 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

The formula of III activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 25mg/mL Tie up the beef extract that element, the dragon fruit polysaccharide that mass concentration is 10mg/mL, mass concentration are 25mg/mL, mass concentration 20mg/ Chitosan that the seawood meal of agar, 12mg/mL that the yeast extract of mL, mass concentration are 10mg/mL, mass concentration are 8mg/mL, Remaining is pine needle leaf juice；Wherein, the processing method of pine needle leaf juice is：By pine needle, enzyme activity intensity be 500U/g cellulase and Water is 30 according to mass ratio:1:100 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

Embodiment 2：

A kind of feed improving camel mammary gland function is present embodiments provided, the feed is by following parts by weight at grouping At：14 parts of additive, 58 parts fermentation mulberry leaf, 37 parts fermentation Folium Pterocaryae, 41 parts fermentation pine needle, 37 parts it is white pierce and 36 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：76 parts of aliphatic acid, 34 parts of mango core essential oil, 29 parts of glossy privet fruit extract, 31 parts of Semen sojae atricolor extract, 33 parts of Thallus Laminariae (Thallus Eckloniae) extract and 35 parts of analysis for soybean powder.

The present embodiment glossy privet fruit extract, Semen sojae atricolor extract, the extracting method of Thallus Laminariae (Thallus Eckloniae) extract and embodiment 1 complete one It causes；And the lecithin content of analysis for soybean powder and embodiment 1 are completely the same.

(1) it is by parts by weight：46 parts of peanut, 34 parts of oil tea slag, 34 parts of wintersweet seed, 23 parts of stool in mice, 17 The suspended sludge in milk wastewater and 15 parts of milk of part are pre-processed respectively, are then mixed, and extraction material is obtained；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 90% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -15 DEG C and suddenly freezes 50min, and the water bath with thermostatic control that temperature is 100 DEG C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 15min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 700w/L；It is ultrasonically treated Temperature is 150 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 30 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 3:The HCl solution that 1 addition mass concentration is 5% is placed in water bath with thermostatic control 40min under 60 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 70min under conditions of temperature is 70 DEG C, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:10 are added percentage by volume as 20% (v/v) Petroleum ether, be placed in temperature be 75 DEG C under conditions of constant temperature extract 80min, obtain extract II after taking ether layer recycling design； Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of the present embodiment step (1) peanut is：By the NaHCO for being 3% with mass concentration after peanut decladding₃ Solution is 4 according to solid-liquid mass ratio:2 mixing, after grinding drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：The NaCl solution for being 3% by oil tea slag and mass concentration It is 4 according to solid-liquid mass ratio:1 is mixed, under conditions of 70 DEG C water-bath 3h filter to take filter residue drying to moisture content be 5% i.e. .

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is % is pulverized, Then it is 8.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 30 according to mass ratio:1 is mixed, 27 NaCl solution that tunning and mass concentration are 3% according to mass ratio is 5 after fermentation by anaerobic fermentation 20d under conditions of DEG C: 1 is mixed, under conditions of 75 DEG C water-bath 3h filter to take filter residue drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 20 according to mass ratio:1 is mixed, and is detested under conditions of 25 DEG C Aerobe fermentation 25d, the NaHCO for being 3% by tunning and mass concentration after fermentation₃Solution is 5 according to mass ratio:2 are mixed, Under conditions of 55 DEG C water-bath 3h filter to take filter residue drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 6.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 23 according to mass ratio:1 is mixed, and ferment 15d under conditions of 28 DEG C, by tunning and matter after fermentation Measure a concentration of 3% NaHCO₃Solution is 6 according to mass ratio:3 are mixed, and water-bath 2h under conditions of 55 DEG C to obtain the final product.

Wherein, the addictive preparation method of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:25 be sufficiently mixed after in 1500r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added into the emulsion of step (2), The vaseline that mixture quality 1/2 is added after being sufficiently stirred, it is 3.22g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The present embodiment feed producing method is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 35 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, is 28 DEG C in temperature, humidity is fermented under conditions of being 27%, fermentation time 23d, in 60 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 25% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 8.7 × 10¹⁰CFU/g saccharomycete and living bacteria count are 9.3 × 10¹⁰CFU/g lactic acid bacterias are 4 according to mass ratio:3 compositions；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 10 by the leaf of fresh weeping willow and shoot:1 mixing It shreds, is then 43 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics, is 28 DEG C in temperature, humidity is It ferments under conditions of 25%, fermentation time 30d is dried to moisture content under the conditions of 60 DEG C and obtained the fermentation for 20% Folium Pterocaryae；The ingredient of the probiotics II is 9.2 × 10 by the living bacteria count after activating¹⁰The mould and effective viable bacteria of CFU/g yeast Number is 9.2 × 10¹⁰CFU/g bacillus subtilises are 4 according to mass ratio:3 compositions；

(3) pine needle prepares fermentation pine needle：It is 35 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 37 DEG C in temperature, humidity is fermented under conditions of being 22%, and fermentation time 33d is dried under the conditions of 65 DEG C to aqueous Rate obtains the fermentation pine needle for 20%；The ingredient of the probiotics III by the living bacteria count after activating be 8.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 9.5 × 10¹⁰CFU/g aspergillus oryzaes are 5 according to mass ratio:3 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 15% are 12% crushes after being thoroughly mixed, and is sieved by 100 mesh screens Choosing, finally adds additive, and drying to moisture content obtains the feed for 5%.

The formula and embodiment 1 of III activation medium of the present embodiment probiotics I, probiotics II and probiotics are completely the same.

Embodiment 3：

A kind of feed improving camel mammary gland function is present embodiments provided, the feed is by following parts by weight at grouping At 11 parts of additive, 51 parts fermentation mulberry leaf, 31 parts fermentation Folium Pterocaryae, 32 parts fermentation pine needle, 27 parts it is white pierce and 31 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：70 parts of aliphatic acid, 29 parts of mango core essential oil, 23 parts of glossy privet fruit extract, 27 parts of Semen sojae atricolor extract, 25 parts of Thallus Laminariae (Thallus Eckloniae) extract and 30 parts of analysis for soybean powder.

(1) it is by parts by weight：36 parts of peanut, 24 parts of oil tea slag, 27 parts of wintersweet seed, 15 parts of stool in mice, 12 The suspended sludge in milk wastewater and 13 parts of milk of part are pre-processed respectively, are then mixed, and extraction material is obtained；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 80% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -17 DEG C and suddenly freezes 40min, and the water bath with thermostatic control that temperature is 95 DEG C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 12min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 500w/L；It is ultrasonically treated Temperature is 140 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 25 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 2:The HCl solution that 1 addition mass concentration is 3% is placed in water bath with thermostatic control 35min under 55 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 65min under conditions of temperature is 60 DEG C, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:9 are added percentage by volume as 15% (v/v) Petroleum ether, be placed in temperature be 70 DEG C under conditions of constant temperature extract 75min, obtain extract II after taking ether layer recycling design； Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of the present embodiment step (1) peanut is：By the NaHCO for being 2% with mass concentration after peanut decladding₃ Solution is 3 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：The NaCl solution for being 2% by oil tea slag and mass concentration It is 3 according to solid-liquid mass ratio:1 is mixed, and it is 4% that water-bath 2.5h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C To obtain the final product.

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is 3% is ground It is broken, it is then 6.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 25 according to mass ratio:1 is mixed, Anaerobic fermentation 18d under conditions of 26 DEG C, after fermentation by tunning and mass concentration be 2% NaCl solution according to mass ratio It is 4:1 is mixed, under conditions of 70 DEG C water-bath 2.5h filter to take filter residue drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 4.1 × 10⁹CFU/ml lactic acid bacterial liquids are 17 according to mass ratio:1 is mixed, and is detested under conditions of 24 DEG C Aerobe fermentation 22d, the NaHCO for being 2% by tunning and mass concentration after fermentation₃Solution is 4 according to mass ratio:1 is mixed, Under conditions of 50 DEG C water-bath 2.5h filter to take filter residue drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 5.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 20 according to mass ratio:1 is mixed, and ferment 12d under conditions of 27 DEG C, by tunning and matter after fermentation Measure a concentration of 2% NaHCO₃Solution is 3 according to mass ratio:2 are mixed, and water-bath 1.5h under conditions of 50 DEG C to obtain the final product.

Wherein, the addictive preparation method of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:22 be sufficiently mixed after in 1200r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added into the emulsion of step (2), The vaseline that mixture quality 1/4 is added after being sufficiently stirred, it is 2.88g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The present embodiment feed producing method is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 32 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, is 26 DEG C in temperature, humidity is fermented under conditions of being 22%, fermentation time 20d, in 57 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 20% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 5.7 × 10¹⁰CFU/g saccharomycete and living bacteria count are 6.3 × 10¹⁰CFU/g lactic acid bacterias are 3 according to mass ratio:2 compositions；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 7 by the leaf of fresh weeping willow and shoot:1 mixing is cut It is broken, it is then 39 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 25 DEG C in temperature, humidity 20% Under conditions of ferment, fermentation time 25d, under the conditions of 57 DEG C drying obtain the fermentation willow to moisture content for 15% Leaf；The ingredient of the probiotics II is 5.2 × 10 by the living bacteria count after activating¹⁰CFU/g yeast is mould and living bacteria count is 5.2×10¹⁰CFU/g bacillus subtilises are 3 according to mass ratio:2 compositions；

(3) pine needle prepares fermentation pine needle：It is 32 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 29 DEG C in temperature, humidity is fermented under conditions of being 20%, and fermentation time 27d is dried under the conditions of 63 DEG C to aqueous Rate obtains the fermentation pine needle for 18%；The ingredient of the probiotics III by the living bacteria count after activating be 4.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 5.5 × 10¹⁰CFU/g aspergillus oryzaes are 3 according to mass ratio:2 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 10% are 7%-12% crushes after being thoroughly mixed, by 70 mesh screens Screening, finally adds additive, and drying to moisture content obtains the feed for 4%.

Control group 1：

This control group is not to each raw material：Peanut, oil tea slag, wintersweet seed, stool in mice, suspended sludge in milk wastewater and milk carry out Pretreatment, but directly raw material is mixed and is produced, other extracting methods are identical with embodiment 1.

Control group 2：

This control group raw material does not use peanut, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts Stool in mice, 9 parts of suspended sludge in milk wastewater and 9 parts of milk mix after being pre-processed respectively, other extracting methods and implementation Example 1 is identical.

Control group 3：

This control group raw material does not use wintersweet seed, i.e., by 12 parts of oil tea slag, 9 parts of stool in mice, 9 parts of Dairy Wastewater Dross and 9 parts of milk mix after being pre-processed respectively, and other extracting methods are identical with embodiment 1.

Control group 4：

This control group raw material does not use stool in mice, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 The suspended sludge in milk wastewater and 9 parts of milk of part mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 5：

This control group raw material does not use suspended sludge in milk wastewater, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet Seed, 9 parts of stool in mice and 9 parts of milk mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 6：

This control group raw material does not use milk, i.e., by 25 parts of peanut, 21 parts of wintersweet seed, 9 parts of stool in mice and 9 parts Suspended sludge in milk wastewater pre-processed respectively after mix, other extracting methods are identical with embodiment 1.

Test experiments 1：

It measures in embodiment 1-3 and control group 1-6, aliphatic acid recovery rate, is specifically shown in Table 1 and GC-MS analysis 1 Hes of embodiment Fatty acid component in control group 1-6, is specifically shown in Table 2：

Table 1

Group	Aliphatic acid recovery rate (%)
		Embodiment 1	57.43
Embodiment 2	58.54
		Embodiment 3	56.34
Control group 1	33.23
		Control group 2	48.76
Control group 3	49,87
		Control group 4	49.76
Control group 5	50.21
		Control group 6	51.24

As seen from the above table, the aliphatic acid recovery rate of embodiment 1-3 is apparently higher than control group 1, and the aliphatic acid of control group 2-6 Recovery rate illustrates to effectively improve using the preprocessing method of raw materials of the application also above control group 1, slightly below embodiment 1-3 The recovery rate of aliphatic acid；Meanwhile it all raw materials of the application being compounded can improve aliphatic acid recovery rate.

Table 2

As seen from the above table, unsaturated fatty acid can be significantly improved using the present processes extraction aliphatic acid：Linoleic acid and The content of oleic acid；And the unsaturated fatty acid of control group 1-6：The content of linoleic plus oleic acid is significantly lower than embodiment 1, illustrates profit Unsaturated fatty acid content can be improved by being extracted aliphatic acid with the cooperation of the raw material of the application and being pre-processed to raw material.

Test experiments 2：(influence of each component content of aliphatic acid to camel mammary gland function in additive)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), is specifically shown in Table 3 in the hunchbacked milk of 300ml tests：

Test group 1：The camel greenfeed of additive+92% prepared by 8% embodiment 1；

Test group 2：The camel greenfeed of additive+92% prepared by 8% control group 1；

Test group 3：The camel greenfeed of additive+92% prepared by 8% control group 2；

Test group 4：The camel greenfeed of additive+92% prepared by 8% control group 3；

Test group 5：The camel greenfeed of additive+92% prepared by 8% control group 4；

Test group 6：The camel greenfeed of additive+92% prepared by 8% control group 5；

Test group 7：The camel greenfeed of additive+92% prepared by 8% control group 6；

Control group：Only feeding camel greenfeed；

Table 3

As seen from the above table, the content of somatic number (SCC) is almost the same in test group 1-7 and control group 0d camel milk, adds After adding the application feed addictive to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group 1-7 camel milk It continuously decreases, the biggest drop is test group 1, and the test group 2-7 ranges of decrease are almost the same, and control group is basically unchanged；Illustrate this Shen Feed addictive please can obviously reduce the content of somatic number (SCC) in hunchbacked milk；To further demonstrate, the feeding of the application The mammary gland function of hunchbacked milk can be improved in feed additives, and each component content of aliphatic acid is affected to the effect of additive in additive.

Test experiments 3：(influence of the additive formulations to camel mammary gland function)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), record the results are shown in Table 4 in the hunchbacked milk of 300ml tests：

Experiment periods feed formula：

Test group A：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight at grouping At：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of sea Analysis for soybean powder with extract and 23 parts.(i.e. the additive of embodiment 1).

Experiment group B：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not Fatty acids are：23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group C：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not Essential oil containing mango core is：65 parts of aliphatic acid, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group D：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing glossy privet fruit extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group E：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing Semen sojae atricolor extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group F：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing Thallus Laminariae (Thallus Eckloniae) extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of black soya bean carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group G：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing analysis for soybean powder：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract With 21 parts of Thallus Laminariae (Thallus Eckloniae) extract；The preparation method of other additives and embodiment 1 are completely the same.

Control group：Only feeding camel greenfeed.

Table 4

As seen from the above table, the content of somatic number (SCC) is almost the same in test group A-G and control group 0d camel milk, adds After adding the application feed addictive to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group A-G camel milk It continuously decreasing, the biggest drop is test group 1, and the test group C-G ranges of decrease are almost the same, and the experiment group B range of decrease is smaller than test group C-G, And control group is basically unchanged；Illustrate that the aliphatic acid in the feed addictive prescription of the application can obviously reduce somatic number in hunchbacked milk (SCC) content；To further demonstrate, the mammary gland function of hunchbacked milk can be improved in the feed addictive prescription of the application, formula Influence of the middle aliphatic acid to camel mammary gland function is maximum, but remaining ingredient also has synergistic effect.

Testing experiment 4：(influence of the feed formula to camel mammary gland function)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (experimental group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), record the results are shown in Table 5 in the hunchbacked milk of 300ml tests：

Experiment periods feed formula：

Experimental group 1：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae of part, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).(i.e. the feed formula of embodiment 1).

Experimental group 2：The feed comprises the following components in parts by weight：41 parts of fermentation mulberry leaf, 27 parts of fermentation willow Leaf, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 3：The feed comprises the following components in parts by weight：8 parts of additive, 27 parts of fermentation Folium Pterocaryae, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 4：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 21 The fermentation pine needle of part, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 5：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae of part, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 6：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae, 21 parts of fermentation pine needle and 20 parts of sand sagebrush (Artemisia filifolia) of part.

Experimental group 7：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae, 21 parts of fermentation pine needle and 19 parts of white thorn of part.

Control group：The feed comprises the following components in parts by weight：19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Table 5

As seen from the above table, the content of somatic number (SCC) is almost the same in experimental group 1-7 and control group 0d camel milk, adds After adding the application feed addictive to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group 1-7 camel milk It continuously decreasing, the biggest drop is experimental group 1, and the experimental group 3-7 ranges of decrease are almost the same, and 2 range of decrease of experimental group is smaller than experimental group 3-7, And control group is basically unchanged；Illustrate that the feed addictive in the feed formula of the application can obviously reduce somatic number in hunchbacked milk (SCC) content；To further demonstrate, the mammary gland function of hunchbacked milk can be improved in the feed formula of the application, is added in formula Influence of the agent to camel mammary gland function is maximum, but remaining ingredient also has synergistic effect.

In conclusion camel mammary gland function can be effectively improved using the feed formula of the present invention, and ingredient is indispensable, The feed of the application is that cobalt blue pigment, additive component are selected from plant source, and feed will not be caused to remain and addition after camel is edible Agent remains, be it is a kind of safely, effectively, the feed of camel mammary gland function can be improved.

Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of feed improving camel mammary gland function, which is characterized in that the feed comprises the following components in parts by weight：8 - 14 parts of additive of part, 41 parts -58 parts of fermentation mulberry leaf, 27 parts -37 parts of fermentation Folium Pterocaryae, 21 parts -41 parts of fermentation pine needle Leaf, 19 parts -37 parts of white thorn and 20 parts -36 parts of sand sagebrush (Artemisia filifolia).

2. the feed according to claim 1 for improving camel mammary gland function, which is characterized in that the additive by weighing as follows Amount part at being grouped as：65 parts -76 parts of aliphatic acid, 23 parts -34 parts of mango core essential oil, the extraction of 18 parts -29 parts of the fruit of glossy privet Object, 23 parts -31 parts of Semen sojae atricolor extract, 21 parts -33 parts of Thallus Laminariae (Thallus Eckloniae) extract and 23 parts -35 parts of analysis for soybean powder.

3. the feed according to claim 2 for improving camel mammary gland function, which is characterized in that the extraction side of the aliphatic acid Method includes the following steps：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 part -23 The stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk of part are pre-processed respectively, are then mixed It closes, obtains extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v): 1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, temperature is directly placed into after taking-up Degree, when the temperature of mixture reaches bath temperature, continues water-bath to thaw, heating in 90 DEG C -100 DEG C of water bath with thermostatic control Heat 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasonic extraction Ultrasonic power density is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；Be ultrasonically treated according to " open ultrasound into Row supersound process 15s- closing ultrasound 5s- opening ultrasounds carry out the processing mode that supersound process 15s " is 1 cycle and are repeated Processing carries out 20-30 circular treatment in total；

(3) mixture after being ultrasonically treated step (2) takes out, and is filtered after being cooled to room temperature, into filtrate according to quality Than for 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in water bath with thermostatic control under 50 DEG C -60 DEG C of temperature condition 30min-40min, it is 1 to be cooled to after room temperature according to volume ratio:1 is added the condition that petroleum ether-ether is 50 DEG C -70 DEG C in temperature Under carry out extraction 60min-70min, obtain extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1: 8-10 be added percentage by volume be 10% (v/v) -20% (v/v) petroleum ether, be placed in temperature be 65 DEG C -75 DEG C under conditions of Constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed After obtain the aliphatic acid.

4. the feed according to claim 3 for improving camel mammary gland function, which is characterized in that step (1) peanut Preprocess method is：By after peanut decladding with mass concentration be 1%-3% NaHCO₃Solution is 2-4 according to solid-liquid mass ratio: 1-2 mix, grinding after drying to moisture content be 3%-5% to obtain the final product.

5. the feed according to claim 3 for improving camel mammary gland function, which is characterized in that step (1) the oil tea slag Preprocess method be：According to solid-liquid mass ratio it is 2-4 by NaCl solution that oil tea slag and mass concentration are 1%-3%:1 carries out Mixing, under conditions of 60 DEG C -70 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

6. the feed according to claim 3 for improving camel mammary gland function, which is characterized in that step (1) the mouse excrement Just preprocess method is：By mouse moisture content be 2%-4% stool in mice pulverize, then with living bacteria count be 5.5 × 10⁹~8.1 × 10⁹CFU/ml bacillus subtilises bacterium solution is 20-30 according to mass ratio:1 is mixed, in 25-27 DEG C of condition NaCl solution that tunning and mass concentration are 1%-3% according to mass ratio is 3- after fermentation by lower anaerobic fermentation 15d-20d 5:1 is mixed, under conditions of 65 DEG C -75 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

7. the feed according to claim 3 for improving camel mammary gland function, which is characterized in that step (1) dairy products are useless The preprocess method of water dross is：It is 2.5 × 10 that Dairy Wastewater, which is filtered, and takes filter residue and living bacteria count⁹~6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 15-20 according to mass ratio:1 is mixed, anaerobic fermentation 20d- under conditions of 23-25 DEG C 25d, by NaHCO that tunning and mass concentration are 1%-3% after fermentation₃Solution is 3-5 according to mass ratio:1-2 is mixed Close, under conditions of 45 DEG C -55 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

8. the feed according to claim 3 for improving camel mammary gland function, which is characterized in that step (1) milk Preprocess method is：It is 3.5 × 10 by milk and living bacteria count⁹~6.7 × 10⁹CFU/ml saccharomycete bacterium solutions are according to mass ratio For 18-23:1 is mixed, and ferment 10d-15d under conditions of 26-28 DEG C, is by tunning and mass concentration after fermentation The NaHCO of 1%-3%₃Solution is 2-6 according to mass ratio:2-3 is mixed, and water-bath 1h-2h is under conditions of 45 DEG C -55 DEG C .

9. preparing the preparation method of the feed as described in claim 1-8, which is characterized in that described method includes following steps：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 30-35 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, is 22 DEG C -28 DEG C in temperature, humidity is fermented under conditions of being 17%-27%, and fermentation time is 18d-23d, drying is that 15%-25% obtains the fermentation mulberry leaf to moisture content under the conditions of 55 DEG C -60 DEG C；The probiotics I Ingredient by the living bacteria count after activating be 3.5 × 10¹⁰~8.7 × 10¹⁰CFU/g saccharomycete and living bacteria count be 3.1 × 10¹⁰~9.3 × 10¹⁰CFU/g lactic acid bacterias are 1-4 according to mass ratio:1-3 is formed；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 5-10 by the leaf of fresh weeping willow and shoot:1 mixing is cut It is broken, it is then 33-43 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 22 DEG C -28 DEG C in temperature, wet Degree ferments under conditions of being 15%-25%, fermentation time 20d-30d, is dried under the conditions of 55 DEG C -60 DEG C and arrives moisture content The fermentation Folium Pterocaryae is obtained for 10%-20%；The ingredient of the probiotics II by the living bacteria count after activating be 2.5 × 10¹⁰~9.2 × 10¹⁰CFU/g yeast is mould and living bacteria count is 2.9 × 10¹⁰~9.2 × 10¹⁰CFU/g bacillus subtilises are pressed It is 2-4 according to mass ratio:1-3 is formed；

(3) pine needle prepares fermentation pine needle：It is 30-35 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 26 DEG C -37 DEG C in temperature, humidity is fermented under conditions of being 18%-22%, fermentation time 20d-33d, at 60 DEG C -65 Drying is that 15%-20% obtains the fermentation pine needle to moisture content under the conditions of DEG C；After the ingredient of the probiotics III is by activating Living bacteria count be 1.6 × 10¹⁰~8.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 1.3 × 10¹⁰~9.5 ×10¹⁰CFU/g aspergillus oryzaes are 2-5 according to mass ratio:1-3 is formed；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, aqueous The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that rate is 5%-15% are 7%-12% crushes after being thoroughly mixed, by 30 mesh -100 Mesh screen screens, and finally adds additive, and drying to moisture content is that 3%-5% obtains the feed.