CN108308115A - A kind of bullog cultivation method - Google Patents
A kind of bullog cultivation method Download PDFInfo
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- CN108308115A CN108308115A CN201810189237.7A CN201810189237A CN108308115A CN 108308115 A CN108308115 A CN 108308115A CN 201810189237 A CN201810189237 A CN 201810189237A CN 108308115 A CN108308115 A CN 108308115A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
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- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Sustainable Development (AREA)
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- Fodder In General (AREA)
Abstract
The invention discloses a kind of bullog cultivation methods, corresponding feed is provided by two growth periods of tadpole period and period of metamorphosis in bullfrog, it effectively improves to the Se content in Frog, improve the immunity and nutritive value of bullfrog, it can have the function that eater's selenium-supply, while there is realistic meaning to the cultivation of the economization of bullfrog.The tadpole period feed is made of Fructus Sophorae mixed extract, corn flour, beancake powder, meat meal tankage, fish meal, duckweed, sweet potato flour, apple powder；Feed is made of maize straw fermented feed, Fel Sus domestica extract, arithoke leaf extract, fish meal, dried silkworm chrysalis meal, corn flour, bean cake powder, fish oil, wheat bran, yeast, chicory leaf, selenium methionine, health-care additive after the metamorphosis.
Description
Technical field
The present invention relates to animal-breeding technical fields, and in particular to a kind of bullog cultivation method.
Background technology
Bullfrog belongs to Amphibia, Anura, Ranidae, is a kind of large edible frog, fine and tender taste, delicious flavour, nutrition
It is abundant, there is certain medical value.Bullfrog, the popular name U.S. water frog, individual is very large, and growth is fast, and yield is high, originates in North America
With the ground such as Mexico, oneself each continent all over the world, is the primary cultivated species in the Rana esculenta of various regions at present.
Bullfrog whole body is all precious, is to integrate edible, medicinal and skin the economic batrachia of large size.Bullfrog meat is superior U.S.
Taste food and advanced health care herbal cuisine, be a kind of high protein, low fat, low cholesterol healthy food；Meanwhile bullfrog meat also has
Very high medical value, it is mild-natured, sweet in flavor, have effects that nourishing removing toxic substances, summer, edible avoidable skin gave birth to sore, and operation is suffered from
Person is edible can to promote wound healing, and liver, consumptive is edible can significantly enhance appetite.In addition, bullfrog skin is good musical instrument material
Material, first-class process hides raw material can also refine advanced open-minded glue；Bull frog oil can make high-quality grease, and bullfrog pituitary is efficiently to hasten parturition
The leftover bits and pieces of hormone, bullfrog can high-quality feed processed.Bullfrog or good experimental animal are the plant protection bodyguards of loyalty.It is artificial to support
It is fast to grow bullfrog growth, yield height, at low cost, value is high, has very high economic benefit.
Selenium is essential trace element in humans and animals body, however research has shown that the universal selenium deficiency of China human mortality, the reason is that people
Soil, water scarcity selenium in the natural environment depended on for existence, directly contribute plant, Se content is relatively low in cereal product, makes
The selenium insufficiency of intake of crowd and animal, and then cause to generate a series of organ injury and disease.
Chinese patent《A kind of efficient circulation cultural method of bullfrog》(number of patent application：CN201410712127.6) open
A kind of cultural method of bullfrog, is fed according to the different growing stages of bullfrog, froglet and at the frog carries out a point pool by weight, and
Feed is fed according to the weight of bullfrog, the efficient high yield of this method, cycling rate is high, is conducive to the yield for improving bullfrog, improves its warp
Ji value.The feed used is based on life food, and the freshness of holding is limited, can direct shadow if the feeding quality fed is bad
Ring the growth performance of bullfrog, high labor cost.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of bullog cultivation methods, using animal and plant extracts as feeding
The main component of material, containing abundant bioactive substance, the nutritive value higher of specific activity body improves the growth of bullfrog
Can, and the selenium element added can be by being converted into the Organic Selenium of needed by human body in animal body.
The present invention can be achieved through the following technical solutions：
A kind of bullog cultivation method, includes the following steps：
(1) after tadpole hatches 1 week, cooked egg Huang is fed, after two weeks, feeds tadpole period feed, feeding volume is that tadpole is total
Start development by metamorphosis after the 3-10% of weight, 60d；
(2) after development by metamorphosis is at froglet 7d, 8 points of every morning feeds feed and earthworm after primary metamorphosis at 4 points in afternoon.
The tadpole period feed：2.1-2.3 parts of Fructus Sophorae mixed extract, 28-32 parts of corn flour, 13-15 parts of beancake powder, meat
4-6 parts of bone meal, 10-14 parts of fish meal, 10-12 parts of duckweed, 4-6 parts of sweet potato flour, 5-7 parts of apple powder；The system of the tadpole period feed
Standby is to be uniformly mixed each raw material, crushed 20 mesh sieve, and 10% water is added and stirs evenly, then puffing through bulking machine, drying.
The Fructus Sophorae mixed extract：
A, by Fructus Sophorae drying and crushing, it is divided into 3 parts of equivalent, respectively fructus sophorae powder A, B, C；
B, fructus sophorae powder A crosses 60 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:6 methanol, microwave refluxing extraction 3 times at 28-32W, often
Secondary 40min, filtering merge extract liquid, dry；
C, fructus sophorae powder B crosses 40 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:3 methanol, the ultrasonic extraction 20min under room temperature, 800W, mistake
Leaching filtrate is dried；
D, fructus sophorae powder C is sieved with 100 mesh sieve, and is placed in autoclave, and it is 1 that solid-liquid ratio, which is added,:12.5 distilled water, in magnetic force
Mixing speed is 120r/min, and at 130 DEG C, Subcritical water chromotagraphy 45min takes extract to dry；
E, it is used after mixing the extract of fructus sophorae powder A, B, C.
Feed after the metamorphosis：20-28 parts of maize straw fermented feed, 0.3-0.5 parts of Fel Sus domestica extract, globe artichoke leaf carry
Take 1.3-1.5 parts of object, 18-22 parts of fish meal, 24-26 parts of dried silkworm chrysalis meal, 18-22 parts of corn flour, 14-18 parts of bean cake powder, fish oil 1.2-
1.4 parts, 8-12 parts of wheat bran, 4-8 parts of yeast, 2-6 parts of chicory leaf, 1-1.2 parts of selenium methionine, 3-5 parts of health-care additive.
The preparation of the froglet feed is, each raw material crushed 0.250mm dusting covers by weight, then will be cooked silkworm chrysalis
It is chopped into powder with chicory leaf, the two adds water to be stirred into grume, then dries, and crushed 0.5cm sieves.
The maize straw fermented feed：5% extra quality zymocyte liquid of addition after maize straw is dried, green formal little slender bamboo wheat 27%,
Careless corn 27% and oil cake of flax seed 3%, in 37-40 DEG C of solid fermentation 36-38h；The extra quality zymocyte liquid be lactobacillus fermenti and
Lactobacillus plantarum, viable count are more than 107cfu/ml。
The Fel Sus domestica extract：Take pig's bile add 2 times amount water be heated to 40 DEG C, with 45% sodium hydroxide tune pH extremely
12, constant temperature saponification 1h, then pH to 2 is reconciled with 1mol/L hydrochloric acid, 30min is stood, supernatant is removed, then adds 2 times of amount water heating boilings
20min is risen, supernatant is removed, boils again, supernatant is removed, residue is taken to dry.
The arithoke leaf extract：Arithoke cured leaf piece is crushed to 20 mesh, it is 1 that solid-liquid ratio, which is added,:8 water, at 700W
Microwave treatment 90s filters to take filtrate drying.
The health-care additive：5-7 parts of purple garlic extract, 18-23 parts of wax gourd, 3-5 parts of Semen Cuscutae, cassia seed 1.3-
1.6 parts, 2-4 parts of Semen sesami nigrum, 3-7 parts of Radix et Rhizoma Gynurae divaricatae powder, 3-4 parts of Siraitia grosvenorii, 0.3-0.4 parts of almond, 0.2-0.3 parts of cymbopogon distans.
The purple garlic extract：By purple garlic peeled wash, smash with high cut disperse emulsification agent it is fair, at 40 DEG C
Pepsin is added and digests 30min, it is 1 to add solid-liquid ratio:5 90% ethanol solution, extracts 2h, through 5000r/ at 25 DEG C
Min centrifuges 15min, and supernatant is taken to dry.
The present invention has following advantageous effect：
The present invention relates to a kind of bullog cultivation method, the bullfrog digestive system development of tadpole period is not perfect, Fructus Sophorae extract
Addition can improve its digestive system function, dramatically increase Growth of Tadpoles performance, plant recovery of nutrient, promote disappearing for bovine IVF embryos
Rate helps to improve development by metamorphosis rate；
The nutriment for the bullfrog offer abundance of maize straw fermented feed of the present invention being incorporated as after metamorphosis, after fermentation
Maize straw is easier to be absorbed and utilized by bullfrog, and fermentation probiotics can stimulate bullfrog to digest and assimilate, moreover it is possible to inhibit other harmful
Germ；
Fel Sus domestica extract of the present invention is added with the cooperation of arithoke leaf extract in period of metamorphosis Bullfrog feedstuff, can improve bullfrog
Production performance, improve lipid metabolism and meat, promote digesting and assimilating for liposoluble vitamin and trace element, ox can be improved
The disease resistance of the frog can especially improve the spawning rate of maturity period bullfrog；Fel Sus domestica extract has lipid-loweringing and liver-protective work
With can improve parenteral function, improve oxidation resistance and metabolism enzyme activity；Arithoke leaf extract has association with Fel Sus domestica extract
Same-action；
Traditional Chinese medicine ingredients in health-care additive of the present invention have the function of antibacterial, anti-inflammatory, inhibition virus, moreover it is possible to adjust bullfrog
Body fat ratio, improve the nutritive value and meat of bullfrog；Have effects that certain cell Proliferation, repair, bullfrog can be reduced
The incidence of common disease；
The addition of purple garlic extract of the present invention can improve the absorption rate of health-care additive, have and promote animal
Growth, improves food conversion ratio, and improves bullfrog survival rate, reduces the effect of aquaculture cost；
The present invention feeds bullfrog by Se-enriched feedstuff, effectively improves to the Se content in Frog, improves the immune of bullfrog
Power and nutritive value can have the function that eater's selenium-supply, while have realistic meaning to the cultivation of the economization of bullfrog.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of bullog cultivation method, mainly includes the following steps that：
(1) after tadpole hatches 1 week, cooked egg Huang is fed, after two weeks, feeds tadpole period feed, feeding volume is that tadpole is total
Start development by metamorphosis after 6.5%, 60d of weight；
(2) after development by metamorphosis is at froglet 7d, 8 points of every morning feeds feed and earthworm after primary metamorphosis at 4 points in afternoon.
A kind of bullog cultivation method of the present embodiment, the tadpole period feed are：2.2 parts of Fructus Sophorae mixed extract, corn flour
30 parts, 14 parts of beancake powder, 5 parts of meat meal tankage, 12 parts of fish meal, 11 parts of duckweed, 5 parts of sweet potato flour, 6 parts of apple powder；The tadpole period is raised
The preparation of material is to be uniformly mixed each raw material, crushed 20 mesh sieve, and 10% water is added and stirs evenly, then puffing through bulking machine,
Drying.
Above-mentioned Fructus Sophorae mixed extract is produced：
(1) by Fructus Sophorae drying and crushing, it is divided into 3 parts of equivalent, respectively fructus sophorae powder A, B, C；
(2) fructus sophorae powder A crosses 60 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:6 methanol, microwave refluxing extraction 3 times at 30W, every time
40min, filtering merge extract liquid, dry；
(3) fructus sophorae powder B crosses 40 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:3 methanol, the ultrasonic extraction 20min under room temperature, 800W,
Filter to take filtrate drying；
(4) fructus sophorae powder C is sieved with 100 mesh sieve, and is placed in autoclave, and it is 1 that solid-liquid ratio, which is added,:12.5 distilled water, in magnetic
Power mixing speed is 120r/min, and at 130 DEG C, Subcritical water chromotagraphy 45min takes extract to dry；
(5) it is used after mixing the extract of fructus sophorae powder A, B, C.
A kind of bullog cultivation method of the present embodiment, feed is after the metamorphosis：24 parts of maize straw fermented feed, pig's bile
0.4 part of extract, 1.4 parts of arithoke leaf extract, 20 parts of fish meal, 25 parts of dried silkworm chrysalis meal, 20 parts of corn flour, 16 parts of bean cake powder, fish oil
1.3 parts, 10 parts of wheat bran, 6 parts of yeast, 4 parts of chicory leaf, 1.1 parts of selenium methionine, 4 parts of health-care additive.
The preparation of above-mentioned froglet feed is：Each raw material be crushed into 0.250mm dusting covers by weight, then the silkworm chrysalis that will be cooked
It is chopped into powder with chicory leaf, the two adds water to be stirred into grume, then dries, and crushed 0.5cm sieves.
Above-mentioned maize straw fermented feed is produced：5% extra quality zymocyte liquid, green formal little slender bamboo wheat are added after maize straw is dried
27%, careless corn 27% and oil cake of flax seed 3%, in 38.5 DEG C of solid fermentation 37h；The extra quality zymocyte liquid be lactobacillus fermenti and
Lactobacillus plantarum, viable count are more than 107cfu/ml。
The preparation of above-mentioned Fel Sus domestica extract：Take pig's bile that 2 times of amount water is added to be heated to 40 DEG C, with 45% sodium hydroxide tune
PH to 12, constant temperature saponification 1h, then pH to 2 is reconciled with 1mol/L hydrochloric acid, 30min is stood, supernatant is removed, then 2 times of amount water is added to add
Heat boiling 20min, removes supernatant, boils again, remove supernatant, residue is taken to dry.
Above-mentioned arithoke leaf extract is produced：Arithoke cured leaf piece is crushed to 20 mesh, it is 1 that solid-liquid ratio, which is added,:8 water,
Microwave treatment 90s under 700W filters to take filtrate drying.
Above-mentioned health-care additive is：6 parts of purple garlic extract, 20.5 parts of wax gourd, 4 parts of Semen Cuscutae, 1.45 parts of cassia seed,
3 parts of Semen sesami nigrum, 5 parts of Radix et Rhizoma Gynurae divaricatae powder, 3.5 parts of Siraitia grosvenorii, 0.35 part of almond, 0.25 part of cymbopogon distans.
The preparation of above-mentioned purple garlic extract：By purple garlic peeled wash, smash with high cut disperse emulsification agent it is fair,
Pepsin is added at 40 DEG C and digests 30min, it is 1 to add solid-liquid ratio:5 90% ethanol solution extracts 2h at 25 DEG C, warp
5000r/min centrifuges 15min, and supernatant is taken to dry.
Embodiment 2
A kind of bullog cultivation method, mainly includes the following steps that：
(1) after tadpole hatches 1 week, cooked egg Huang is fed, after two weeks, feeds tadpole period feed, feeding volume is that tadpole is total
Start development by metamorphosis after 3%, 60d of weight；
(2) after development by metamorphosis is at froglet 7d, 8 points of every morning feeds feed and earthworm after primary metamorphosis at 4 points in afternoon.
A kind of bullog cultivation method of the present embodiment, the tadpole period feed are：2.1 parts of Fructus Sophorae mixed extract, corn flour
28 parts, 13 parts of beancake powder, 4 parts of meat meal tankage, 10 parts of fish meal, 10 parts of duckweed, 4 parts of sweet potato flour, 5 parts of apple powder；The tadpole period is raised
The preparation of material is to be uniformly mixed each raw material, crushed 20 mesh sieve, and 10% water is added and stirs evenly, then puffing through bulking machine,
Drying.
Above-mentioned Fructus Sophorae mixed extract is produced：
(1) by Fructus Sophorae drying and crushing, it is divided into 3 parts of equivalent, respectively fructus sophorae powder A, B, C；
(2) fructus sophorae powder A crosses 60 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:6 methanol, microwave refluxing extraction 3 times at 28W, every time
40min, filtering merge extract liquid, dry；
(3) fructus sophorae powder B crosses 40 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:3 methanol, the ultrasonic extraction 20min under room temperature, 800W,
Filter to take filtrate drying；
(4) fructus sophorae powder C is sieved with 100 mesh sieve, and is placed in autoclave, and it is 1 that solid-liquid ratio, which is added,:12.5 distilled water, in magnetic
Power mixing speed is 120r/min, and at 130 DEG C, Subcritical water chromotagraphy 45min takes extract to dry；
(5) it is used after mixing the extract of fructus sophorae powder A, B, C.
A kind of bullog cultivation method of the present embodiment, feed is after the metamorphosis：20 parts of maize straw fermented feed, pig's bile
0.3 part of extract, 1.3 parts of arithoke leaf extract, 18 parts of fish meal, 24 parts of dried silkworm chrysalis meal, 18 parts of corn flour, 14 parts of bean cake powder, fish oil
1.2 parts, 8 parts of wheat bran, 4 parts of yeast, 2 parts of chicory leaf, 1 part of selenium methionine, 3 parts of health-care additive.
The preparation of above-mentioned froglet feed is：Each raw material be crushed into 0.250mm dusting covers by weight, then the silkworm chrysalis that will be cooked
It is chopped into powder with chicory leaf, the two adds water to be stirred into grume, then dries, and crushed 0.5cm sieves.
Above-mentioned maize straw fermented feed is produced：5% extra quality zymocyte liquid, green formal little slender bamboo wheat are added after maize straw is dried
27%, careless corn 27% and oil cake of flax seed 3%, in 37 DEG C of solid fermentation 36h；The extra quality zymocyte liquid is lactobacillus fermenti and plant
Object lactobacillus, viable count are more than 107cfu/ml。
The preparation of above-mentioned Fel Sus domestica extract：Take pig's bile that 2 times of amount water is added to be heated to 40 DEG C, with 45% sodium hydroxide tune
PH to 12, constant temperature saponification 1h, then pH to 2 is reconciled with 1mol/L hydrochloric acid, 30min is stood, supernatant is removed, then 2 times of amount water is added to add
Heat boiling 20min, removes supernatant, boils again, remove supernatant, residue is taken to dry.
Above-mentioned arithoke leaf extract is produced：Arithoke cured leaf piece is crushed to 20 mesh, it is 1 that solid-liquid ratio, which is added,:8 water,
Microwave treatment 90s under 700W filters to take filtrate drying.
Above-mentioned health-care additive is：It is 5 parts of purple garlic extract, 18 parts of wax gourd, 3 parts of Semen Cuscutae, 1.3 parts of cassia seed, black
2 parts of sesame, 3 parts of Radix et Rhizoma Gynurae divaricatae powder, 3 parts of Siraitia grosvenorii, 0.3 part of almond, 0.2 part of cymbopogon distans.
The preparation of above-mentioned purple garlic extract：By purple garlic peeled wash, smash with high cut disperse emulsification agent it is fair,
Pepsin is added at 40 DEG C and digests 30min, it is 1 to add solid-liquid ratio:5 90% ethanol solution extracts 2h at 25 DEG C, warp
5000r/min centrifuges 15min, and supernatant is taken to dry.
Embodiment 3
A kind of bullog cultivation method, mainly includes the following steps that：
(1) after tadpole hatches 1 week, cooked egg Huang is fed, after two weeks, feeds tadpole period feed, feeding volume is that tadpole is total
Start development by metamorphosis after 10%, 60d of weight；
(2) after development by metamorphosis is at froglet 7d, 8 points of every morning feeds feed and earthworm after primary metamorphosis at 4 points in afternoon.
A kind of bullog cultivation method of the present embodiment, the tadpole period feed are：2.3 parts of Fructus Sophorae mixed extract, corn flour
32 parts, 15 parts of beancake powder, 6 parts of meat meal tankage, 14 parts of fish meal, 12 parts of duckweed, 6 parts of sweet potato flour, 7 parts of apple powder；The tadpole period is raised
The preparation of material is to be uniformly mixed each raw material, crushed 20 mesh sieve, and 10% water is added and stirs evenly, then puffing through bulking machine,
Drying.
Above-mentioned Fructus Sophorae mixed extract is produced：
(1) by Fructus Sophorae drying and crushing, it is divided into 3 parts of equivalent, respectively fructus sophorae powder A, B, C；
(2) fructus sophorae powder A crosses 60 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:6 methanol, microwave refluxing extraction 3 times at 32W, every time
40min, filtering merge extract liquid, dry；
(3) fructus sophorae powder B crosses 40 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:3 methanol, the ultrasonic extraction 20min under room temperature, 800W,
Filter to take filtrate drying；
(4) fructus sophorae powder C is sieved with 100 mesh sieve, and is placed in autoclave, and it is 1 that solid-liquid ratio, which is added,:12.5 distilled water, in magnetic
Power mixing speed is 120r/min, and at 130 DEG C, Subcritical water chromotagraphy 45min takes extract to dry；
(5) it is used after mixing the extract of fructus sophorae powder A, B, C.
A kind of bullog cultivation method of the present embodiment, feed is after the metamorphosis：28 parts of maize straw fermented feed, pig's bile
0.5 part of extract, 1.5 parts of arithoke leaf extract, 22 parts of fish meal, 26 parts of dried silkworm chrysalis meal, 22 parts of corn flour, 18 parts of bean cake powder, fish oil
1.4 parts, 12 parts of wheat bran, 8 parts of yeast, 6 parts of chicory leaf, 1.2 parts of selenium methionine, 5 parts of health-care additive.
The preparation of above-mentioned froglet feed is：Each raw material be crushed into 0.250mm dusting covers by weight, then the silkworm chrysalis that will be cooked
It is chopped into powder with chicory leaf, the two adds water to be stirred into grume, then dries, and crushed 0.5cm sieves.
Above-mentioned maize straw fermented feed is produced：5% extra quality zymocyte liquid, green formal little slender bamboo wheat are added after maize straw is dried
27%, careless corn 27% and oil cake of flax seed 3%, in 40 DEG C of solid fermentation 38h；The extra quality zymocyte liquid is lactobacillus fermenti and plant
Object lactobacillus, viable count are more than 107cfu/ml。
The preparation of above-mentioned Fel Sus domestica extract：Take pig's bile that 2 times of amount water is added to be heated to 40 DEG C, with 45% sodium hydroxide tune
PH to 12, constant temperature saponification 1h, then pH to 2 is reconciled with 1mol/L hydrochloric acid, 30min is stood, supernatant is removed, then 2 times of amount water is added to add
Heat boiling 20min, removes supernatant, boils again, remove supernatant, residue is taken to dry.
Above-mentioned arithoke leaf extract is produced：Arithoke cured leaf piece is crushed to 20 mesh, it is 1 that solid-liquid ratio, which is added,:8 water,
Microwave treatment 90s under 700W filters to take filtrate drying.
Above-mentioned health-care additive is：It is 7 parts of purple garlic extract, 23 parts of wax gourd, 5 parts of Semen Cuscutae, 1.6 parts of cassia seed, black
4 parts of sesame, 7 parts of Radix et Rhizoma Gynurae divaricatae powder, 4 parts of Siraitia grosvenorii, 0.4 part of almond, 0.3 part of cymbopogon distans.
The preparation of above-mentioned purple garlic extract：By purple garlic peeled wash, smash with high cut disperse emulsification agent it is fair,
Pepsin is added at 40 DEG C and digests 30min, it is 1 to add solid-liquid ratio:5 90% ethanol solution extracts 2h at 25 DEG C, warp
5000r/min centrifuges 15min, and supernatant is taken to dry.
Place should be selected in the place that topography is higher, has abundant water resources, does not pollute, it is ensured that have abundant water resources, water is changed conveniently, in water
Without poisonous and harmful substances such as ammonia nitrogen, nitrite, heavy metals, the pH value of water source and water should be in 6.5-8.5.
Comparative example 1
Difference lies in tadpole period feed does not add Fructus Sophorae mixed extract with embodiment 1.
Comparative example 2
Difference lies in feed does not apply health-care additive after metamorphosis with embodiment 1.
Comparative example 3
Difference lies in period of metamorphosis Bullfrog feedstuff does not apply Fel Sus domestica extract and arithoke leaf extract with embodiment 1.
Effect assessment
Place should be selected in the place that topography is higher, has abundant water resources, does not pollute, it is ensured that have abundant water resources, water is changed conveniently, in water
Without poisonous and harmful substances such as ammonia nitrogen, nitrite, heavy metals, the pH value of water source and water should be 6.5~8.5.
In accordance with the above-mentioned embodiment 1~3 and the method for comparative example 1~3 cultivates wood frog's fallopian tube, is tracked since hatching, by metamorphosis
Development reaches a body maturation, each method is shared to divide this six groups of Examples 1 to 3, comparative example 1~3 as one group.
Correlative data analysis is as follows in breeding process：
As seen from the above table, the data of above example 1~3 are not much different, and than comparative example than get well, illustrate using this
The cultural method of invention, can effectively improve the tadpole development by metamorphosis rate of bullfrog, absorption rate growth rate, disease rate of descent,
Spawning rate, protein content.
Embodiment 1 is more apparent than comparative example 1 to improve development by metamorphosis rate, and the addition of Fructus Sophorae extract can improve digestive system
The digestive system function of imperfect bovine IVF embryos phase is developed, promotes digestibility of the bovine IVF embryos to tadpole period feed, significantly increases
Add Growth of Tadpoles performance, plant recovery of nutrient；
After the metamorphosis of embodiment 1 in feed health-care additive, traditional Chinese medicine ingredients have antibacterial, work that is anti-inflammatory, inhibiting virus
With can adjust the body fat ratio of bullfrog, improve the relative amount of protein, reduce total cholesterol, triacylglycerol, lipoprotein cholesterol
Level, significantly improve the nutritive value and meat edibility of bullfrog；Also have effects that certain cell Proliferation, repair,
The incidence of common disease in bullfrog growth and development process can be reduced；The addition of ingredient purple garlic extract can increase health care
Residence time of the additive in bullfrog digestive system, keep health-care effect lasting, to more improve health-care additive than comparative example 2
Absorption rate；
Embodiment 1 is used cooperatively this process, pig gall than the Fel Sus domestica extract of comparative example more than 3 and arithoke leaf extract
Juice extract is added with the cooperation of arithoke leaf extract in period of metamorphosis Bullfrog feedstuff, can significantly improve the production performance of bullfrog, change
Digesting and assimilating for kind lipid metabolism and meat, promotion liposoluble vitamin and trace element, increases protein content, improves ox
The disease resistance of the frog, more particularly to significantly improve the spawning rate of maturity period bullfrog；
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.
Claims (10)
1. a kind of bullog cultivation method, which is characterized in that include the following steps：
(1) after tadpole hatches 1 week, cooked egg Huang is fed, after two weeks, feeds tadpole period feed, feeding volume is tadpole total weight
3-10%, start development by metamorphosis after 60d；
(2) after development by metamorphosis is at froglet 7d, 8 points of every morning feeds feed and earthworm after primary metamorphosis at 4 points in afternoon.
2. a kind of bullog cultivation method according to claim 1, which is characterized in that the tadpole period feed：The Fructus Sophorae mixes
2.1-2.3 parts of extract, 28-32 parts of corn flour, 13-15 parts of beancake powder, 4-6 parts of meat meal tankage, 10-14 parts of fish meal, duckweed 10-12
Part, 4-6 parts of sweet potato flour, 5-7 parts of apple powder；The preparation of the tadpole period feed is to be uniformly mixed each raw material, crushed 20 mesh
Sieve is added 10% water and stirs evenly, then puffing through bulking machine, drying.
3. a kind of bullog cultivation method according to claim 2, which is characterized in that the Fructus Sophorae mixed extract：
(1) by Fructus Sophorae drying and crushing, it is divided into 3 parts of equivalent, respectively fructus sophorae powder A, B, C；
(2) fructus sophorae powder A crosses 60 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:6 methanol, microwave refluxing extraction 3 times at 28-32W, every time
40min, filtering merge extract liquid, dry；
(3) fructus sophorae powder B crosses 40 mesh sieve, and it is 1 that solid-liquid ratio, which is added,:3 methanol, the ultrasonic extraction 20min under room temperature, 800W, filtering
Filtrate is taken to dry；
(4) fructus sophorae powder C is sieved with 100 mesh sieve, and is placed in autoclave, and it is 1 that solid-liquid ratio, which is added,:12.5 distilled water is stirred in magnetic force
Speed is mixed as 120r/min, at 130 DEG C, Subcritical water chromotagraphy 45min takes extract to dry；
(5) it is used after mixing the extract of fructus sophorae powder A, B, C.
4. a kind of bullog cultivation method according to claim 1, which is characterized in that feed after the metamorphosis：Maize straw
20-28 parts of fermented feed, 0.3-0.5 parts of Fel Sus domestica extract, 1.3-1.5 parts of arithoke leaf extract, 18-22 parts of fish meal, silkworm chrysalis
24-26 parts of powder, 18-22 parts of corn flour, 14-18 parts of bean cake powder, 1.2-1.4 parts of fish oil, 8-12 parts of wheat bran, 4-8 parts of yeast, chrysanthemum
2-6 parts of lettuce leaf, 1-1.2 parts of selenium methionine, 3-5 parts of health-care additive.
5. a kind of bullog cultivation method according to claim 4, which is characterized in that the preparation of the froglet feed is to press
Each raw material crushed 0.250mm dusting covers by parts by weight, then cooked silkworm chrysalis and chicory leaf are chopped into powder, and the two adds water to stir
It uniformly at grume, then dries, crushed 0.5cm sieves.
6. a kind of bullog cultivation method according to claim 4, which is characterized in that the maize straw fermented feed：It will
5% extra quality zymocyte liquid, green formal little slender bamboo wheat 27%, careless corn 27% and oil cake of flax seed 3%, at 37-40 DEG C is added in maize straw after drying
Solid fermentation 36-38h；The extra quality zymocyte liquid is lactobacillus fermenti and lactobacillus plantarum, and viable count is more than 107cfu/ml。
7. a kind of bullog cultivation method according to claim 4, which is characterized in that the Fel Sus domestica extract：Take pig gall
Juice adds 2 times of amount water to be heated to 40 DEG C, with 45% sodium hydroxide tune pH to 12, constant temperature saponification 1h, then with the conciliation of 1mol/L hydrochloric acid
PH to 2 stands 30min, removes supernatant, then adds 2 times of amount water ebuillition of heated 20min, removes supernatant, boils again, removes
Supernatant takes residue to dry.
8. a kind of bullog cultivation method according to claim 4, which is characterized in that the arithoke leaf extract：By arithoke
Cured leaf piece is crushed to 20 mesh, and it is 1 that solid-liquid ratio, which is added,:8 water, the microwave treatment 90s at 700W filter to take filtrate drying.
9. a kind of bullog cultivation method according to claim 4, which is characterized in that the health-care additive：Purple garlic
5-7 parts of extract, 18-23 parts of wax gourd, 3-5 parts of Semen Cuscutae, 1.3-1.6 parts of cassia seed, 2-4 parts of Semen sesami nigrum, Radix et Rhizoma Gynurae divaricatae powder 3-7
Part, 3-4 parts of Siraitia grosvenorii, 0.3-0.4 parts of almond, 0.2-0.3 parts of cymbopogon distans.
10. a kind of bullog cultivation method according to claim 9, which is characterized in that the purple garlic extract：It will be purple
Skin garlic peeling clean, smash with high cut disperse emulsification agent it is fair, at 40 DEG C be added pepsin digest 30min, add material
Liquor ratio is 1:5 90% ethanol solution, 2h is extracted at 25 DEG C, centrifuges 15min through 5000r/min, supernatant is taken to dry.
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| CN109662060A (en) * | 2018-11-21 | 2019-04-23 | 岳阳雪晖农业科技有限公司 | A kind of bullog cultivation method |
| CN116098112A (en) * | 2021-11-09 | 2023-05-12 | 中洋渔业(清远)有限公司 | Method for recycling three-dimensional breeding water body of bullfrog fish and shrimp |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662060A (en) * | 2018-11-21 | 2019-04-23 | 岳阳雪晖农业科技有限公司 | A kind of bullog cultivation method |
| CN116098112A (en) * | 2021-11-09 | 2023-05-12 | 中洋渔业(清远)有限公司 | Method for recycling three-dimensional breeding water body of bullfrog fish and shrimp |
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