CN108184766A

CN108184766A - A kind of method for breeding for improving camel mammary gland function

Info

Publication number: CN108184766A
Application number: CN201810141443.0A
Authority: CN
Inventors: 隆杰
Original assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Current assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Priority date: 2018-02-11
Filing date: 2018-02-11
Publication date: 2018-06-22

Abstract

The present invention relates to camel cultural technique fields, more particularly to a kind of method for breeding for improving camel mammary gland function, the method for breeding of the application is carried out using stable breeding mode, and emulsion is protected in camel latter half of gestation artificial feed, and camel hormone in vivo balance is maintained to prevent lactagogue is too fast from camel breast being caused to lump；0 10d after camel lambing extends hunchbacked lamb and inhales the time 6h 9h for permitting female camel breast, mainly stimulate female camel lactation, and hunchbacked lamb is allowed to be emptied milk as early as possible at this time, prevents stifled milk, while feed the feed I for being suitble to camel taste；11d starts after camel lambing, shorten hunchbacked lamb and inhale the time for permitting female camel breast, it is that hunchbacked lamb eats excessive and influences the collection to hunchbacked milk in order to prevent at this time, simultaneously, feed I, the feed II of feeding process are formulated rationally, and the cultivation of manual intervention camel, rational formula camel feed can be accomplished so that camel will not block up milk, dyssecretosis.

Description

A kind of method for breeding for improving camel mammary gland function

【Technical field】

The present invention relates to camel cultural technique field, more particularly to a kind of method for breeding for improving camel mammary gland function.

【Background technology】

The various nutritional ingredients that bactrian camel milk and camel dairy products are needed containing human body are one of best wholefoods of the mankind, Bactrian camel milk is developed and used to improving household's actions, grows local economy, development characteristic dairy products industry, meeting the market demand has Significance.Since reducing the process and development with camel milk due to domestic camel cultivation quantity, the price for causing camel is also continuous It is soaring, so as to bring new vitality to the development of cultivation camel industry.But it is current camel milk development that the camel output of milk is not high Bottleneck problem in the process.

The output of milk of camel depends primarily on the supply situation of feed and water, improves nutritive value in feed and is produced to improving Milk amount plays a very important role.In camel breeding process, mostly use, which is put in a suitable place to breed, cultivates camel, this is because camel It is higher to the taste requirements of feed, if feed will not be eaten by being unable to effective stimulus camel taste bud camel, during conventional cultivation, Camel is selective with seasonal variations, and during feeding to the selection of forage grass, especially likes seasonal forage. And the more annual herbage of preference feeding；Due to camel " particular about food " at present, there are no the research to its formula forage, and match Square feed, which has, to be convenient for carrying, feeds advantage easy to operate.And during female camel is put in a suitable place to breed, since camel growing environment is disliked It is bad, it is mostly grown in desert, female camel is easy to that mammary gland blockage, ovary can be led to because the feed formula of intake is unreasonable The problems such as function reduction, these problems will influence camel lactation；And what is taken camel is the mode put in a suitable place to breed more, if it is desired to Improve camel mammary gland function, then it is contemplated that the latter half of gestation, lactation initial stage in camel carry out manual intervention to camel breast, Because the adverse reactions such as obstructed milk, stifled milk, mammary gland jam, mastitis easily occur for female camel at this time.

【Invention content】

In view of the above, it is necessary to a kind of method for breeding for improving camel mammary gland function be provided, safe and effective can be carried The mammary gland function of high camel ensures that nursing period mother camel mammary gland is unimpeded.

In order to achieve the above objectives, the technical solution adopted in the present invention is：

A kind of method for breeding for improving camel mammary gland function, described method includes following steps：

(1) since the preceding 40d of the camel expected date of childbirth, primary shield emulsion is fed to the gestational period camel of pregnancy daily；It is described The additive amount for protecting emulsion is 300ml/ head -500ml/ heads；

(2) 0-10d after camel whelps gives camel feeding feed according to the additive amount of -1800g/, 1500g/ heads daily I, and hunchbacked lamb is allowed to inhale daily and permits female camel breast 6h-9h；The feed I according to mass ratio is 4-7 by coarse fodder and fine fodder：20-25 It is mixed to prepare；

(3) 11d starts after camel whelps, and camel feeding is given to raise according to the additive amount of -2200g/, 1800g/ heads daily Material II, and hunchbacked lamb is allowed to inhale daily and permits female camel breast 2h-3h；The feed II according to mass ratio is 7-10 by coarse fodder and fine fodder： 2-4 is mixed to prepare.

Further, step (2) the shield emulsion is 25-30 according to mass ratio by pine needle water, wormwood juice and Kelp Powder: 15-20:1-3 is formed.

Further, the step (2), (3) coarse fodder by maize flour, Cottonseed Meal and oil tea slag according to mass ratio be 2-4: 5-9:3-5 is formed.

Further, the step (2), (3) fine fodder comprise the following components in parts by weight：8 parts -14 parts of addition Agent, 41 parts -58 parts fermentation mulberry leaf, 27 parts -37 parts fermentation Folium Pterocaryae, 21 parts -41 parts of fermentation pine needle, 19 parts -37 parts White thorn and 20 parts -36 parts of sand sagebrush (Artemisia filifolia).

Further, the additive comprises the following components in parts by weight：65 parts -76 parts of aliphatic acid, 23 parts -34 parts Mango core essential oil, 18 parts -29 parts of glossy privet fruit extract, 23 parts -31 parts of Semen sojae atricolor extract, 21 parts -33 parts of kelp carry Take object and 23 parts -35 parts of analysis for soybean powder.

Further, the preparation method for stating aliphatic acid includes the following steps：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 The stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk of -23 parts of part are pre-processed respectively, Ran Houjin Row mixing obtains extraction material；

(2) by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v) according to mass ratio It is 1:1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, is directly put after taking-up Enter and thaw, heat in the water bath with thermostatic control that temperature is 90 DEG C -100 DEG C, when the temperature of mixture reaches bath temperature, continue Heating water bath 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasound carries The ultrasonic power density taken is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；It is ultrasonically treated according to " opening is super It is that 1 processing mode recycled carries out that sound, which be ultrasonically treated 15s- and close ultrasound 5s- and open ultrasound to carry out supersound process 15s ", Reprocessing carries out 20-30 circular treatment in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in thermostatted water under 50 DEG C -60 DEG C of temperature condition 30min-40min is bathed, it is 1 to be cooled to after room temperature according to volume ratio:1 adds in petroleum ether-ether in the item that temperature is 50 DEG C -70 DEG C Extraction 60min-70min is carried out under part, extract I is obtained after taking ether layer recycling design；It is according to solid-liquid mass ratio into filter residue 1:8-10 adds in the petroleum ether that percentage by volume is 10% (v/v) -20% (v/v), is placed in the condition that temperature is 65 DEG C -75 DEG C Lower constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed The aliphatic acid is obtained after conjunction.

The preprocess method of step (1) peanut is：By after peanut decladding with mass concentration be 1%-3% NaHCO₃ Solution is 2-4 according to solid-liquid mass ratio:1-2 mix, grinding after drying to moisture content for 3%-5% to obtain the final product.

The preprocess method of step (1) the oil tea slag is：By the NaCl solution of oil tea slag and mass concentration for 1%-3% It is 2-4 according to solid-liquid mass ratio:1 is mixed, and water-bath 2h-3h filters to take filter residue drying to containing under conditions of 60 DEG C -70 DEG C Water rate for 3%-5% to obtain the final product.

The preprocess method of step (1) stool in mice is：The stool in mice that mouse moisture content is 2%-4% is ground It is broken, it is then 5.5 × 10 with living bacteria count⁹~8.1 × 10⁹CFU/ml bacillus subtilises bacterium solution is 20- according to mass ratio 30:1 is mixed, and tunning and mass concentration are by the anaerobic fermentation 15d-20d under conditions of 25-27 DEG C after fermentation The NaCl solution of 1%-3% is 3-5 according to mass ratio:1 is mixed, and water-bath 2h-3h is filtered to take under conditions of 65 DEG C -75 DEG C Filter residue drying to moisture content for 3%-5% to obtain the final product.

The preprocess method of step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with effectively Viable count is 2.5 × 10⁹~6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 15-20 according to mass ratio:1 is mixed, in 23-25 Anaerobic fermentation 20d-25d under conditions of DEG C, by the NaHCO of tunning and mass concentration for 1%-3% after fermentation₃Solution according to Mass ratio is 3-5:1-2 is mixed, and water-bath 2h-3h, which filters to take filter residue and dries to moisture content, under conditions of 45 DEG C -55 DEG C is 3%-5% to obtain the final product.

The preprocess method of step (1) milk is：It is 3.5 × 10 by milk and living bacteria count⁹~6.7 × 10⁹CFU/ml saccharomycete bacterium solution is 18-23 according to mass ratio:1 is mixed, and ferment 10d-15d under conditions of 26-28 DEG C, By the NaHCO of tunning and mass concentration for 1%-3% after fermentation₃Solution is 2-6 according to mass ratio:2-3 is mixed, Water-bath 1h-2h under conditions of 45 DEG C -55 DEG C to obtain the final product.

The present invention has the advantages that：

1st, the method for breeding of the application is carried out using stable breeding mode, is protected emulsion in camel latter half of gestation artificial feed, is maintained Camel hormone in vivo balance prevents lactagogue is too fast from camel breast being caused to lump；The 0-10d after camel lambing extends hunchbacked lamb and inhales fair mother The time 6h-9h of camel breast mainly stimulates female camel lactation, and hunchbacked lamb is allowed to be emptied milk as early as possible, prevents at this time Stifled milk, while feed the feed I for being suitble to camel taste；11d starts after camel lambing, shortens hunchbacked lamb and inhales fair female camel breast Time, be that hunchbacked lamb eats excessive and influences the collection to hunchbacked milk in order to prevent at this time, meanwhile, at this time according to female camel lactation It is required that addition feed II；The raising camel output of milk is played in feed I, feed II is the essence of present inventor's independent research Material, can accomplish manual intervention, rational formula camel feed so that camel will not block up milk, dyssecretosis.

2nd, the shield emulsion of the application is made of pine needle water, wormwood juice and Kelp Powder, pine needle water and wormwood juice in these ingredients In containing abundant flavones, the ovarian function of can effectively protect camel enables it to balance camel hormone in vivo in the gestational period, will not It stimulates the secretion of milk too early, leads to stifled milk phenomenon；Contain a large amount of salt in Kelp Powder, the mouthfeel of shield emulsion can be increased, be more suitable for camel and drink.

3rd, the fine fodder of the application is made of additive, fermentation mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, Bai Ci and sand sagebrush (Artemisia filifolia), Wherein, additive component is the active ingredient for improving camel mammary gland function of the application applicant's independent research；Mulberry leaf, willow Containing abundant protein in leaf, pine needle, mulberry leaf, Folium Pterocaryae, pine needle smell faint scent are pleasant, while mouthfeel is soft, pollution It is few, while promote the secretion of camel estrogen also containing abundant flavone component energy effective protection camel ovary in pine needle, these Herbage has strong ferment fragrance that can increase feed palatability after everfermentation；And mulberry leaf, Folium Pterocaryae, pine needle fermentation in feed The small-molecule substances such as amino acid therein, vitamin are adequately released afterwards, are suitble to the female camel constitution just produced；This Shen Also containing certain herbaceous plants with big flowers, thorn and sand sagebrush (Artemisia filifolia), these ingredients contain abundant crude protein in vain in feed please, can meet female camel to nonirrigated farmland herbage The basic nutrition material requisite of crude protein, feed can be applied more easily after rational formula, processing and be cultivated with camel.Feed In mulberry leaf, Folium Pterocaryae, probiotics used all have passed through activation to pine needle during the fermentation, different journeys in activation medium Degree adds white mulberry vein-juice, willow leaf juice, pine needle juice etc., these ingredients can be probiotic enriched, accelerates probiotics to mulberry leaf, willow The fermentation of leaf, pine needle.

4th, the feed addictive of the application is by aliphatic acid, mango core essential oil, glossy privet fruit extract, Semen sojae atricolor extract, kelp Extract and analysis for soybean powder form, and in ingredient, aliphatic acid is the aliphatic acid that applicant independently extracts, wherein containing compared with horn of plenty not Saturated fatty acid, unrighted acid Linoleic acid content is 69.47% or so, and oleic acid content is 22.02% or so, intake Amount is more will not to lead to that camel cholesterol is excessively high, the fat effect for blocking mammary gland of camel；Contain abundant oil in mango core essential oil The plurality of active ingredients such as acid, palmitic acid and Octadec-9-enoic Acid contain abundant oleic acid, leukotrienes, sweet dew in the fruit of glossy privet Alcohol, these ingredients all have the function of to remove biological interior free yl, inhibit bacteria, the growth of saccharomycete and fungi, can effectively rise To antioxidation, abundant protein, general flavone are contained in Semen sojae atricolor extract；Contain abundant thick egg in Thallus Laminariae (Thallus Eckloniae) extract In vain, core yellow cellulose content, vitamin B1；The ovary work(of animal can be protected in analysis for soybean powder containing abundant lecithin these ingredients Can, ovary is promoted effectively to secrete estrogen so as to balance camel hormone in vivo content, allow camel mammogenesis faster；So as to The purpose of protection camel mammary gland function is played in interaction in terms of dredging mammary gland pipeline, promoting hormonal balance two.

【Specific embodiment】

All features or disclosed all methods disclosed in this specification or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.

Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each Feature is an example in a series of equivalent or similar characteristics.

Embodiment 1：

Present embodiments provide a kind of method for breeding for improving camel mammary gland function：

(1) since the preceding 40d of the camel expected date of childbirth, primary shield emulsion is fed to the gestational period camel of pregnancy daily；It is described The additive amount for protecting emulsion is 300ml/ heads；

(2) 0-10d after camel whelps daily according to the additive amount of 1500g/ to camel feeding feed I, and allows hunchbacked lamb It inhales daily and permits female camel breast 6h；The feed I according to mass ratio is 4 by coarse fodder and fine fodder：20 are mixed to prepare；

(3) 11d starts after camel whelps, and daily according to the additive amount of 1800g/ to camel feeding feed II, and allows Hunchbacked lamb inhales daily permits female camel breast 2h；The feed II according to mass ratio is 7 by coarse fodder and fine fodder：2 are mixed to prepare.

Shield emulsion is 25 according to mass ratio by pine needle water, wormwood juice and Kelp Powder in the step (2):15:1 composition.

1. the processing method of pine needle water is in shield emulsion：By fresh needles shred afterwards with enzyme activity intensity be 500U/g fiber Plain enzyme is 30 according to mass ratio:1 is mixed, and then adds the water of 4 times of mass parts of mixture quality, is put into after being sufficiently stirred Temperature is to digest 3d in 37 DEG C of insulating box, is filtered to remove filter residue and obtains the pine needle water.

2. the processing method of wormwood juice is in shield emulsion：By fresh wormwood shred afterwards with enzyme activity intensity be 500U/g fiber Plain enzyme is 25 according to mass ratio:1 is mixed, and then adds the water of 3 times of mass parts of mixture quality, is put into after being sufficiently stirred Temperature is to digest 2d in 37 DEG C of insulating box, is filtered to remove filter residue and obtains the pine needle water.

3. the processing method of Kelp Powder is in shield emulsion：The dry kelp that moisture content is 3% is crushed, is then passed through 100 mesh screens screen to obtain the Kelp Powder.

Coarse fodder in the step (2), (3) is 2 according to mass ratio by maize flour, Cottonseed Meal and oil tea slag:5:3 compositions.

Fine fodder in the step (2), (3) comprises the following components in parts by weight：The feed of camel mammary gland function is improved, The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 parts of fermentation Folium Pterocaryae, 21 The fermentation pine needle of part, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of Thallus Laminariae (Thallus Eckloniae) extract and 23 parts of analysis for soybean powder.

The extracting method of above-mentioned glossy privet fruit extract is：The fruit of glossy privet seed that moisture content is 10% is pulverized and volume basis The butanol that number is 10% is according to solid-liquid mass ratio 1:1 is mixed, temperature be 70 DEG C stirred in water bath temperature soak 3 times, every time 40min, filtering, merging filtrate carries out vacuum distillation concentration until paste, the relative density of paste is 1.66g/cm³Obtain institute Glossy privet fruit extract is stated, extract mid-oleic is 41.65%；Linolenic acid content is 34.87%；Mannitol content is 11.21%.

The extracting method of above-mentioned Semen sojae atricolor extract is：After black soya bean is soaked with water according to mass ratio be 1:1 adds water to be ground Mill, is then placed in 90 DEG C of water-baths and preheats, and it is 5% then to add with the mass percent of mixed liquor equal quality ratio NaHCO₃Solution is then placed in constant temperature blender with magnetic force and shakes, and protein is made to be dissolved in 3% NaHCO₃Solution, 1000r/min 30min is centrifuged, supernatant is collected, obtains albumin；Take the sodium hydroxide solution for centrifuging lower floor's substance with mass percent is 3% Then mixing collects supernatant according to same method (centrifugal rotational speed, time are identical), obtains globulin；Residue is added in again Percentage by volume is 75% ethanol solution, and dissolving collects supernatant, obtains protein,alcohol-soluble；Then by above-mentioned albumin, ball It is 10% that albumen and protein,alcohol-soluble, which mix and dry moisture content, obtains Semen sojae atricolor extract, protein contains in Semen sojae atricolor extract It measures as 754.25mg/g；General flavone content is 216.25mg/g.

The extracting method of above-mentioned Thallus Laminariae (Thallus Eckloniae) extract is：Fresh Laminaria Japonica is shredded, with organic solvent mixed liquor according to mass ratio It is 1:2 are mixed, organic solvent mixed liquor by percentage by volume be 30% ethyl acetate and 65% ethyl alcohol according to quality Than being 1:1 is mixed, and refluxing extraction is then carried out in refluxing extraction device, and Extracting temperature is 105 DEG C, extraction time 2h, It filters to take filtrate and carries out vacuum distillation concentration until paste, the relative density of paste is 1.49g/cm³, obtain the kelp and carry Take object, crude protein content is 241.04mg/g in extract, core yellow cellulose content is 96.24mg/g, vitamin B1 content is 25.07mg/g。

The lecithin content of above-mentioned analysis for soybean powder is 31.11%.

The preparation method of aliphatic acid includes the following steps in above-mentioned additive：

(1) it is by parts by weight：25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts of stool in mice, 9 parts Suspended sludge in milk wastewater and 9 parts of milk pre-processed respectively, then mixed, obtain extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -20 DEG C and suddenly freezes 30min, water bath with thermostatic control at a temperature of 90 °C is directly placed into after taking-up Middle defrosting, heating when the temperature of mixture reaches bath temperature, continue heating water bath 10min；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 400w/L；It is ultrasonically treated Temperature is 130 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 20 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1:The HCl solution that 1 addition mass concentration is 2% is placed in water bath with thermostatic control 30min under 50 DEG C of temperature condition, cools down After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 60min- under conditions of being 50 DEG C in temperature 70min obtains extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:8, which add in percentage by volumes, is The petroleum ether of 10% (v/v) is placed in constant temperature under conditions of temperature is 65 DEG C and extracts 70min, obtained after taking ether layer recycling design Extract II；The aliphatic acid will be obtained after extract I and extract II uniformly mixing.

The preprocess method of step (1) peanut is in the present embodiment addictive preparation method：By after peanut decladding with quality A concentration of 1% NaHCO3 solution is 2 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content for 3% to obtain the final product.

The preprocess method of step (1) oil tea slag is in the present embodiment addictive preparation method：Oil tea slag and quality is dense It according to solid-liquid mass ratio is 2 to spend for 1% NaCl solution:1 is mixed, and water-bath 2h filters to take filter residue baking under conditions of 60 DEG C Moisture content is done as 3% to obtain the final product.

The preprocess method of step (1) stool in mice is in the present embodiment addictive preparation method：It is by mouse moisture content 2% stool in mice is pulverized, and is then 5.5 × 10 with living bacteria count⁹CFU/ml bacillus subtilis bacterium solutions are according to mass ratio It is 20:1 is mixed, the anaerobic fermentation 15d under conditions of 25 DEG C, by tunning and mass concentration is 1% after fermentation NaCl solution is 3 according to mass ratio:1 is mixed, and water-bath 2h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C is 3% to obtain the final product.

The preprocess method of step (1) suspended sludge in milk wastewater is in the present embodiment addictive preparation method：By Dairy Wastewater It is 2.5 × 10 to be filtered and take filter residue and living bacteria count⁹CFU/ml lactic acid bacterial liquids are 15 according to mass ratio:1 is mixed, The anaerobic fermentation 20d under conditions of 23 DEG C, by NaHCO that tunning and mass concentration are 1% after fermentation₃Solution is according to quality Than being 3:1 is mixed, and water-bath 2h filters to take filter residue drying to moisture content as 3% under conditions of 45 DEG C to obtain the final product.

The preprocess method of step (1) milk is in the present embodiment addictive preparation method：By milk and living bacteria count It is 3.5 × 10⁹CFU/ml saccharomycete bacterium solution is 18 according to mass ratio:1 is mixed, and ferment 10d under conditions of 26 DEG C, fermentation Afterwards by NaHCO that tunning and mass concentration are 1%₃Solution is 2 according to mass ratio:2 are mixed, in 45 DEG C DEG C of condition Lower water-bath 1h to obtain the final product.

Wherein, the preparation method of the additive of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:20 be sufficiently mixed after in 1000r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added in into the emulsion of step (2), The vaseline of mixture quality 1/5 is added after being sufficiently stirred, relative density is made as 2.31g/cm³Medicinal extract obtain described add Add agent.

The preparation method of the feed of the present embodiment is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 30 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, be 22 DEG C in temperature, humidity is fermented under conditions of being 17%, fermentation time 18d, in 55 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 15% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 3.5 × 10¹⁰CFU/g saccharomycete and living bacteria count are 3.1 × 10¹⁰CFU/g lactic acid bacterias are 1 according to mass ratio:1 composition；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 5 by the leaf of fresh weeping willow and shoot:1 mixing is cut It is broken, it is then 33 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 22 DEG C in temperature, humidity 15% Under conditions of ferment, fermentation time 20d, drying is 10% to obtain the fermentation willow to moisture content under the conditions of 55 DEG C Leaf；The ingredient of the probiotics II is 2.5 × 10 by the living bacteria count after activating¹⁰CFU/g yeast is mould and living bacteria count is 2.9×10¹⁰CFU/g bacillus subtilises are 2 according to mass ratio:1 composition；

(3) pine needle prepares fermentation pine needle：It is 30 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 26 DEG C in temperature, humidity is fermented under conditions of being 18%, and fermentation time 20d is dried under the conditions of 60 DEG C to aqueous Rate obtains the fermentation pine needle for 15%；The ingredient of the probiotics III by the living bacteria count after activating for 1.6 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 1.3 × 10¹⁰CFU/g aspergillus oryzaes are 2 according to mass ratio:1 composition；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 5% are 7% crushes after being thoroughly mixed, and is screened by 30 mesh screens, Additive is finally added, drying to moisture content obtains the feed for 3%.

The formula of the present embodiment probiotics I activation mediums is (total volume 1L)：Mass concentration is the fiber of 20mg/mL Element, mass concentration be 5mg/mL glucose, mass concentration be 21mg/mL beef extract, mass concentration be 15mg/mL albumen Seawood meal that agar that peptone, mass concentration are 10mg/mL, mass concentration are 8mg/mL, remaining be white mulberry vein-juice；Wherein, white mulberry vein-juice Processing method be：According to mass ratio it is 20 by cellulase and water that mulberry leaf, enzyme activity intensity are 500U/g:1:80 are mixed It closes, is put into 37 DEG C of insulating boxs and reacts 3d, filtering removal filtrate is made.

The formula of II activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 20mg/mL Tie up the beef extract that element, the aloe polysaccharide that mass concentration is 10mg/mL, mass concentration are 25mg/mL, mass concentration 15mg/mL Yeast extract, mass concentration be 9mg/mL seawood meal, mass concentration be 15mg/mL agar, remaining be willow leaf juice；Its In, the processing method of willow leaf juice is：It is according to mass ratio by cellulase and water that Folium Pterocaryae, enzyme activity intensity are 500U/g 30:1:90 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

The formula of III activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 25mg/mL Tie up the beef extract that element, the dragon fruit polysaccharide that mass concentration is 10mg/mL, mass concentration are 25mg/mL, mass concentration 20mg/ Chitosan that agar that the yeast extract of mL, mass concentration are 10mg/mL, the seawood meal of 12mg/mL, mass concentration are 8mg/mL, Remaining is pine needle leaf juice；Wherein, the processing method of pine needle leaf juice is：By pine needle, enzyme activity intensity be 500U/g cellulase and Water is 30 according to mass ratio:1:100 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

Embodiment 2：

(1) since the preceding 40d of the camel expected date of childbirth, primary shield emulsion is fed to the gestational period camel of pregnancy daily；It is described The additive amount for protecting emulsion is 500ml/ heads；

(2) 0-10d after camel whelps daily according to the additive amount of 1800g/ to camel feeding feed I, and allows hunchbacked lamb It inhales daily and permits female camel breast 6h-9h；The feed I according to mass ratio is 7 by coarse fodder and fine fodder：25 are mixed to prepare；

(3) 11d starts after camel whelps, and daily according to the additive amount of 2200g/ to camel feeding feed II, and allows Hunchbacked lamb inhales daily permits female camel breast 2h-3h；The feed II according to mass ratio is 10 by coarse fodder and fine fodder：4 are mixed to prepare.

Shield emulsion is 30 according to mass ratio by pine needle water, wormwood juice and Kelp Powder in the step (2):20:3 compositions.

It is completely the same to protect pine needle water, wormwood juice, the processing method of Kelp Powder and embodiment 1 in emulsion.

Coarse fodder in the step (2), (3) is 4 according to mass ratio by maize flour, Cottonseed Meal and oil tea slag:9:5 compositions.

Fine fodder in the step (2), (3) comprises the following components in parts by weight：14 parts of additive, 58 parts of fermentation Mulberry leaf, 37 parts of fermentation Folium Pterocaryae, 41 parts of fermentation pine needle, 37 parts of white thorn and 36 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：76 parts of aliphatic acid, 34 parts of mango core essential oil, 29 parts of glossy privet fruit extract, 31 parts of Semen sojae atricolor extract, 33 parts of Thallus Laminariae (Thallus Eckloniae) extract and 35 parts of analysis for soybean powder.

The present embodiment glossy privet fruit extract, Semen sojae atricolor extract, the extracting method of Thallus Laminariae (Thallus Eckloniae) extract and embodiment 1 complete one It causes；And the lecithin content of analysis for soybean powder and embodiment 1 are completely the same.

(1) it is by parts by weight：46 parts of peanut, 34 parts of oil tea slag, 34 parts of wintersweet seed, 23 parts of stool in mice, 17 The suspended sludge in milk wastewater and 15 parts of milk of part are pre-processed respectively, are then mixed, and obtain extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 90% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -15 DEG C and suddenly freezes 50min, and the water bath with thermostatic control that temperature is 100 DEG C is directly placed into after taking-up Middle defrosting, heating when the temperature of mixture reaches bath temperature, continue heating water bath 15min；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 700w/L；It is ultrasonically treated Temperature is 150 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 30 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 3:The HCl solution that 1 addition mass concentration is 5% is placed in water bath with thermostatic control 40min under 60 DEG C of temperature condition, cools down After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 70min under conditions of being 70 DEG C in temperature, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:10 add in percentage by volume as 20% (v/v) Petroleum ether, be placed in temperature and extract 80min for constant temperature under conditions of 75 DEG C, extract II is obtained after taking ether layer recycling design； The aliphatic acid will be obtained after extract I and extract II uniformly mixing.

The preprocess method of the present embodiment step (1) peanut is：By after peanut decladding with mass concentration be 3% NaHCO₃ Solution is 4 according to solid-liquid mass ratio:2 mixing, after grinding drying to moisture content for 5% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：By the NaCl solution that oil tea slag and mass concentration are 3% It is 4 according to solid-liquid mass ratio:1 is mixed, and water-bath 3h filters to take filter residue drying to moisture content as 5% i.e. under conditions of 70 DEG C .

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is % is pulverized, Then it is 8.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 30 according to mass ratio:1 is mixed, 27 NaCl solution that tunning and mass concentration are 3% according to mass ratio is 5 after fermentation by anaerobic fermentation 20d under conditions of DEG C: 1 is mixed, and water-bath 3h filters to take filter residue drying to moisture content as 5% under conditions of 75 DEG C to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 20 according to mass ratio:1 is mixed, and is detested under conditions of 25 DEG C Aerobe fermentation 25d, by NaHCO that tunning and mass concentration are 3% after fermentation₃Solution is 5 according to mass ratio:2 are mixed, Water-bath 3h filters to take filter residue drying to moisture content as 5% under conditions of 55 DEG C to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 6.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 23 according to mass ratio:1 is mixed, and ferment 15d under conditions of 28 DEG C, by tunning and matter after fermentation Measure a concentration of 3% NaHCO₃Solution is 6 according to mass ratio:3 are mixed, and water-bath 2h under conditions of 55 DEG C to obtain the final product.

Wherein, the preparation method of the additive of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:25 be sufficiently mixed after in 1500r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added in into the emulsion of step (2), The vaseline of mixture quality 1/2 is added after being sufficiently stirred, relative density is made as 3.22g/cm³Medicinal extract obtain described add Add agent.

The preparation method of the feed of the present embodiment is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 35 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, be 28 DEG C in temperature, humidity is fermented under conditions of being 27%, fermentation time 23d, in 60 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 25% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 8.7 × 10¹⁰CFU/g saccharomycete and living bacteria count are 9.3 × 10¹⁰CFU/g lactic acid bacterias are 4 according to mass ratio:3 compositions；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 10 by the leaf of fresh weeping willow and shoot:1 mixing It shreds, is then 43 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics, is 28 DEG C in temperature, humidity is It ferments under conditions of 25%, fermentation time 30d is dried to moisture content under the conditions of 60 DEG C and obtained the fermentation for 20% Folium Pterocaryae；The ingredient of the probiotics II is 9.2 × 10 by the living bacteria count after activating¹⁰The mould and effective viable bacteria of CFU/g yeast Number is 9.2 × 10¹⁰CFU/g bacillus subtilises are 4 according to mass ratio:3 compositions；

(3) pine needle prepares fermentation pine needle：It is 35 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 37 DEG C in temperature, humidity is fermented under conditions of being 22%, and fermentation time 33d is dried under the conditions of 65 DEG C to aqueous Rate obtains the fermentation pine needle for 20%；The ingredient of the probiotics III by the living bacteria count after activating for 8.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 9.5 × 10¹⁰CFU/g aspergillus oryzaes are 5 according to mass ratio:3 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 15% are 12% crushes after being thoroughly mixed, and is sieved by 100 mesh screens Choosing, finally adds additive, and drying to moisture content obtains the feed for 5%.

The formula and embodiment 1 of III activation medium of the present embodiment probiotics I, probiotics II and probiotics are completely the same.

Embodiment 3：

(1) since the preceding 40d of the camel expected date of childbirth, primary shield emulsion is fed to the gestational period camel of pregnancy daily；It is described The additive amount for protecting emulsion is 400ml/ heads；

(2) 0-10d after camel whelps daily according to the additive amount of 1600g/ to camel feeding feed I, and allows hunchbacked lamb It inhales daily and permits female camel breast 8h；The feed I according to mass ratio is 5 by coarse fodder and fine fodder：22 are mixed to prepare；

(3) 11d starts after camel whelps, and daily according to the additive amount of 2000g/ to camel feeding feed II, and allows Hunchbacked lamb inhales daily permits female camel breast 2.5h；The feed II according to mass ratio is 9 by coarse fodder and fine fodder：3 are mixed to prepare.

Shield emulsion is 27 according to mass ratio by pine needle water, wormwood juice and Kelp Powder in the step (2):17:2 compositions.

Coarse fodder in the step (2), (3) is 3 according to mass ratio by maize flour, Cottonseed Meal and oil tea slag:7:4 compositions.

Fine fodder in the step (2), (3) comprises the following components in parts by weight：11 parts of additive, 51 parts of fermentation Mulberry leaf, 31 parts of fermentation Folium Pterocaryae, 32 parts of fermentation pine needle, 27 parts of white thorn and 31 parts of sand sagebrush (Artemisia filifolia).

The additive of the present embodiment comprises the following components in parts by weight：70 parts of aliphatic acid, 29 parts of mango core essential oil, 23 parts of glossy privet fruit extract, 27 parts of Semen sojae atricolor extract, 25 parts of Thallus Laminariae (Thallus Eckloniae) extract and 30 parts of analysis for soybean powder.

(1) it is by parts by weight：36 parts of peanut, 24 parts of oil tea slag, 27 parts of wintersweet seed, 15 parts of stool in mice, 12 The suspended sludge in milk wastewater and 13 parts of milk of part are pre-processed respectively, are then mixed, and obtain extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 80% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -17 DEG C and suddenly freezes 40min, and the water bath with thermostatic control that temperature is 95 DEG C is directly placed into after taking-up Middle defrosting, heating when the temperature of mixture reaches bath temperature, continue heating water bath 12min；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 500w/L；It is ultrasonically treated Temperature is 140 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 25 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 2:The HCl solution that 1 addition mass concentration is 3% is placed in water bath with thermostatic control 35min under 55 DEG C of temperature condition, cools down After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 65min under conditions of being 60 DEG C in temperature, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:9 add in percentage by volume as 15% (v/v) Petroleum ether, be placed in temperature and extract 75min for constant temperature under conditions of 70 DEG C, extract II is obtained after taking ether layer recycling design； The aliphatic acid will be obtained after extract I and extract II uniformly mixing.

The preprocess method of the present embodiment step (1) peanut is：By after peanut decladding with mass concentration be 2% NaHCO₃ Solution is 3 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content for 4% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：By the NaCl solution that oil tea slag and mass concentration are 2% It is 3 according to solid-liquid mass ratio:1 is mixed, and it is 4% that water-bath 2.5h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C To obtain the final product.

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is 3% is ground It is broken, it is then 6.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 25 according to mass ratio:1 is mixed, The anaerobic fermentation 18d under conditions of 26 DEG C, by NaCl solution that tunning and mass concentration are 2% according to mass ratio after fermentation It is 4:1 is mixed, and water-bath 2.5h filters to take filter residue drying to moisture content as 4% under conditions of 70 DEG C to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 4.1 × 10⁹CFU/ml lactic acid bacterial liquids are 17 according to mass ratio:1 is mixed, and is detested under conditions of 24 DEG C Aerobe fermentation 22d, by NaHCO that tunning and mass concentration are 2% after fermentation₃Solution is 4 according to mass ratio:1 is mixed, Water-bath 2.5h filters to take filter residue drying to moisture content as 4% under conditions of 50 DEG C to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 5.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 20 according to mass ratio:1 is mixed, and ferment 12d under conditions of 27 DEG C, by tunning and matter after fermentation Measure a concentration of 2% NaHCO₃Solution is 3 according to mass ratio:2 are mixed, and water-bath 1.5h under conditions of 50 DEG C to obtain the final product.

Wherein, the preparation method of the additive of the present embodiment is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and mango core essential oil:22 be sufficiently mixed after in 1200r/min It is stirred to obtain emulsion under the action of blender；

(3) glossy privet fruit extract, Semen sojae atricolor extract, Thallus Laminariae (Thallus Eckloniae) extract and analysis for soybean powder are added in into the emulsion of step (2), The vaseline of mixture quality 1/4 is added after being sufficiently stirred, relative density is made as 2.88g/cm³Medicinal extract obtain described add Add agent.

The preparation method of the feed of the present embodiment is：

(1) mulberry leaf prepare fermentation mulberry leaf：New fresh mulberry leaf is shredded, is then 32 according to mulberry leaf and probiotics mass ratio:1 adds Add probiotics I ingredients, be 26 DEG C in temperature, humidity is fermented under conditions of being 22%, fermentation time 20d, in 57 DEG C of items Drying obtains the fermentation mulberry leaf to moisture content for 20% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 5.7 × 10¹⁰CFU/g saccharomycete and living bacteria count are 6.3 × 10¹⁰CFU/g lactic acid bacterias are 3 according to mass ratio:2 compositions；

(2) Folium Pterocaryae prepares fermentation Folium Pterocaryae：According to mass ratio it is 7 by the leaf of fresh weeping willow and shoot:1 mixing is cut It is broken, it is then 39 according to mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 25 DEG C in temperature, humidity 20% Under conditions of ferment, fermentation time 25d, drying is 15% to obtain the fermentation willow to moisture content under the conditions of 57 DEG C Leaf；The ingredient of the probiotics II is 5.2 × 10 by the living bacteria count after activating¹⁰CFU/g yeast is mould and living bacteria count is 5.2×10¹⁰CFU/g bacillus subtilises are 3 according to mass ratio:2 compositions；

(3) pine needle prepares fermentation pine needle：It is 32 by pine needle and probiotics mass ratio:1 addition III ingredient of probiotics, It it is 29 DEG C in temperature, humidity is fermented under conditions of being 20%, and fermentation time 27d is dried under the conditions of 63 DEG C to aqueous Rate obtains the fermentation pine needle for 18%；The ingredient of the probiotics III by the living bacteria count after activating for 4.5 × 10¹⁰CFU/g bacillus subtilises and living bacteria count are 5.5 × 10¹⁰CFU/g aspergillus oryzaes are 3 according to mass ratio:2 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment mulberry leaf, fermentation Folium Pterocaryae, fermentation pine needle, The sand sagebrush (Artemisia filifolia) that the white thorn and moisture content that moisture content is 10% are 7%-12% crushes after being thoroughly mixed, by 70 mesh screens Screening, finally adds additive, and drying to moisture content obtains the feed for 4%.

Control group 1：

This control group is not to each raw material：Peanut, oil tea slag, wintersweet seed, stool in mice, suspended sludge in milk wastewater and milk carry out Pretreatment, but directly raw material is mixed and is produced, other extracting methods are identical with embodiment 1.

Control group 2：

This control group raw material does not use peanut, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts Stool in mice, 9 parts of suspended sludge in milk wastewater and 9 parts of milk mix after being pre-processed respectively, and other extracting methods are with implementing Example 1 is identical.

Control group 3：

This control group raw material does not use wintersweet seed, i.e., by 12 parts of oil tea slag, 9 parts of stool in mice, 9 parts of Dairy Wastewater Scum silica frost and 9 parts of milk mix after being pre-processed respectively, and other extracting methods are identical with embodiment 1.

Control group 4：

This control group raw material does not use stool in mice, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 The suspended sludge in milk wastewater and 9 parts of milk of part mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 5：

This control group raw material does not use suspended sludge in milk wastewater, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet Seed, 9 parts of stool in mice and 9 parts of milk mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 6：

This control group raw material does not use milk, i.e., by 25 parts of peanut, 21 parts of wintersweet seed, 9 parts of stool in mice and 9 parts Suspended sludge in milk wastewater pre-processed respectively after mix, other extracting methods are identical with embodiment 1.

Test experiments 1：

It measures in embodiment 1-3 and control group 1-6, aliphatic acid recovery rate, is specifically shown in Table 1 and GC-MS analysis 1 Hes of embodiment Fatty acid component in control group 1-6, is specifically shown in Table 2：

Table 1

As seen from the above table, the aliphatic acid recovery rate of embodiment 1-3 is apparently higher than control group 1, and the aliphatic acid of control group 2-6 Recovery rate illustrates to effectively improve using the preprocessing method of raw materials of the application also above control group 1, slightly below embodiment 1-3 The recovery rate of aliphatic acid；Meanwhile by all raw materials of the application carry out compounding can improve aliphatic acid recovery rate.

Table 2

As seen from the above table, unrighted acid can be significantly improved using the present processes extraction aliphatic acid：Linoleic acid and The content of oleic acid；And the unrighted acid of control group 1-6：The content of linoleic plus oleic acid is significantly lower than embodiment 1, illustrates profit Unsaturated fatty acid content can be improved by being extracted aliphatic acid with the raw material cooperation of the application and raw material being pre-processed.

Test experiments 2：(influence of each component content of aliphatic acid to camel mammary gland function in additive)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), is specifically shown in Table 3 in the hunchbacked milk of 300ml tests：

Test group 1：The camel greenfeed of additive+92% prepared by 8% embodiment 1；

Test group 2：The camel greenfeed of additive+92% prepared by 8% control group 1；

Test group 3：The camel greenfeed of additive+92% prepared by 8% control group 2；

Test group 4：The camel greenfeed of additive+92% prepared by 8% control group 3；

Test group 5：The camel greenfeed of additive+92% prepared by 8% control group 4；

Test group 6：The camel greenfeed of additive+92% prepared by 8% control group 5；

Test group 7：The camel greenfeed of additive+92% prepared by 8% control group 6；

Control group：Only feeding camel greenfeed；

Table 3

As seen from the above table, the content of somatic number (SCC) is basically identical in test group 1-7 and control group 0d camel milk, adds After the application feed addictive is added to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group 1-7 camel milk It continuously decreases, the biggest drop is test group 1, and the test group 2-7 ranges of decrease are basically identical, and control group is basically unchanged；Illustrate this Shen Feed addictive please can obviously reduce the content of somatic number (SCC) in hunchbacked milk；So as to further demonstrate, the feeding of the application Feed additives can improve the mammary gland function of hunchbacked milk, and each component content of aliphatic acid is affected to the effect of additive in additive.

Test experiments 3：(influence of the additive formulations to camel mammary gland function)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), record the results are shown in Table 4 in the hunchbacked milk of 300ml tests：

Experiment periods feed formula：

Test group A：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight into grouping Into：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of sea Analysis for soybean powder with extract and 23 parts.(i.e. the additive of embodiment 1).

Experiment group B：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not Fatty acids are：23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group C：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not Essential oil containing mango core is：65 parts of aliphatic acid, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group D：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing glossy privet fruit extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 23 parts of Semen sojae atricolor extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group E：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing Semen sojae atricolor extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 21 parts of kelp carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group F：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing Thallus Laminariae (Thallus Eckloniae) extract：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of black soya bean carry Take object and 23 parts of analysis for soybean powder；The preparation method of other additives and embodiment 1 are completely the same.

Test group G：The camel greenfeed of 8% additive+92%；Wherein, additive by following parts by weight ingredient not It is containing analysis for soybean powder：65 parts of aliphatic acid, 23 parts of mango core essential oil, 18 parts of glossy privet fruit extract, 23 parts of Semen sojae atricolor extract With 21 parts of Thallus Laminariae (Thallus Eckloniae) extract；The preparation method of other additives and embodiment 1 are completely the same.

Control group：Only feeding camel greenfeed.

Table 4

As seen from the above table, the content of somatic number (SCC) is basically identical in test group A-G and control group 0d camel milk, adds After the application feed addictive is added to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group A-G camel milk It continuously decreasing, the biggest drop is test group 1, and the test group C-G ranges of decrease are basically identical, and the experiment group B range of decrease is smaller than test group C-G, And control group is basically unchanged；Illustrate that the aliphatic acid in the feed addictive prescription of the application can obviously reduce somatic number in hunchbacked milk (SCC) content；So as to further demonstrate, the feed addictive prescription of the application can improve the mammary gland function of hunchbacked milk, formula Influence of the middle aliphatic acid to camel mammary gland function is maximum, but remaining ingredient also has synergistic effect.

Testing experiment 4：(influence of the feed formula to camel mammary gland function)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (experimental group 1-7) and control Group carries out the raising of 30d by a definite date, 0d, 10d, 20d before feeding, 30d mornings 9:00-10:Hunchbacked milk is taken between 00 The content of somatic number (SCC), record the results are shown in Table 5 in the hunchbacked milk of 300ml tests：

Experiment periods feed formula：

Experimental group 1：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae of part, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).(i.e. the feed formula of embodiment 1).

Experimental group 2：The feed comprises the following components in parts by weight：41 parts of ferment mulberry leaf, 27 parts of fermentation willow Leaf, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 3：The feed comprises the following components in parts by weight：8 parts of additive, 27 parts of fermentation Folium Pterocaryae, 21 parts of fermentation pine needle, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 4：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 21 The fermentation pine needle of part, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 5：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae of part, 19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Experimental group 6：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae, 21 parts of fermentation pine needle and 20 parts of sand sagebrush (Artemisia filifolia) of part.

Experimental group 7：The feed comprises the following components in parts by weight：8 parts of additive, 41 parts of fermentation mulberry leaf, 27 The fermentation Folium Pterocaryae, 21 parts of fermentation pine needle and 19 parts of white thorn of part.

Control group：The feed comprises the following components in parts by weight：19 parts of white thorn and 20 parts of sand sagebrush (Artemisia filifolia).

Table 5

As seen from the above table, the content of somatic number (SCC) is basically identical in experimental group 1-7 and control group 0d camel milk, adds After the application feed addictive is added to feed 10d, 20d, 30d, the content of somatic number (SCC) in test group 1-7 camel milk It continuously decreasing, the biggest drop is experimental group 1, and the experimental group 3-7 ranges of decrease are basically identical, and 2 range of decrease of experimental group is smaller than experimental group 3-7, And control group is basically unchanged；Illustrate that the feed addictive in the feed formula of the application can obviously reduce somatic number in hunchbacked milk (SCC) content；So as to further demonstrate, the feed formula of the application can improve the mammary gland function of hunchbacked milk, be added in formula Influence of the agent to camel mammary gland function is maximum, but remaining ingredient also has synergistic effect.

Raising experiment：

Control group A：It is fed without using the shield emulsion of the application, other method for breeding, feed formula, feed preparation side Method and embodiment 1 are completely the same.

Control group B：Coarse fodder is used only when being fed using feed I, feed II without using fine fodder, other method for breeding, Feed formula, feed producing method and embodiment 1 are completely the same.

Control group C：Fine fodder is used only when being fed using feed I, feed II without using coarse fodder, other method for breeding, Feed formula, feed producing method and embodiment 1 are completely the same.

Control group D：It is fed without using the shield emulsion, feed I, feed II of the application, other method for breeding, feed are matched Side, feed producing method and embodiment 1 are completely the same.

70 milk production phase camels are divided into 7 groups, every group of method for breeding by embodiment 1-3 and control group A-D to camel into Row raising, in feeding according to the method described above to camel farrowing 20d, 30d mornings 9:00-10:Hunchbacked milk 300ml is taken to survey between 00 The content of somatic number (SCC), record the results are shown in Table 6 in the hunchbacked milk of examination：

Table 6

As seen from the above table, somatic number (SCC) content is below control group in the hunchbacked milk of embodiment 1-3 20d, 30d A-D illustrates that using the method for breeding of the application camel mammary gland function can be significantly improved, in control group A-D, control group C camel milk Middle somatic number (SCC) content is minimum, and somatic number (SCC) content is more slightly higher than control group C in control group A camel milk, control group B camels Somatic number (SCC) content and control group C difference are little in milk, illustrate, are played in the method for breeding of the application and improve camel The method for breeding of mammary gland function is：Use concentrated feed and shield emulsion.

In conclusion camel mammary gland function, and the feed of the application can be effectively improved using the method for breeding of the present invention Formula is greenfeed, and additive formulations are taken from plant source, and any feed will not be caused to remain and remained with additive, be one Kind safely, effectively, the method for breeding of camel mammary gland function content can be improved.

Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of method for breeding for improving camel mammary gland function, which is characterized in that described method includes following steps：

(1) since the preceding 40d of the camel expected date of childbirth, primary shield emulsion is fed to the gestational period camel of pregnancy daily；The shield breast The additive amount of agent is 300ml/ head -500ml/ heads；

(2) 0-10d after camel whelps, daily according to the additive amount of -1800g/, 1500g/ heads to camel feeding feed I, and Hunchbacked lamb is allowed to inhale daily and permits female camel breast 6h-9h；The feed I according to mass ratio is 4-7 by coarse fodder and fine fodder：20-25 is mixed It is made；

(3) 11d starts after camel whelps, daily according to the additive amount of -2200g/, 1800g/ heads to camel feeding feed II, And hunchbacked lamb is allowed to inhale daily and permits female camel breast 2h-3h；The feed II according to mass ratio is 7-10 by coarse fodder and fine fodder：2-4 is mixed It closes and is made.

A kind of 2. method for breeding for improving camel mammary gland function according to claim 1, which is characterized in that the step (2) It is 25-30 according to mass ratio that emulsion, which is protected, by pine needle water, wormwood juice and Kelp Powder:15-20:1-3 is formed.

3. a kind of method for breeding for improving camel mammary gland function according to claim 1, which is characterized in that the step (2), (3) coarse fodder is 2-4 according to mass ratio by maize flour, Cottonseed Meal and oil tea slag:5-9:3-5 is formed.

4. a kind of method for breeding for improving camel mammary gland function according to claim 1, which is characterized in that the step (2), (3) fine fodder comprises the following components in parts by weight：8 parts -14 parts of additive, 41 parts -58 parts of fermentation mulberry leaf, 27 part -37 The fermentation Folium Pterocaryae of part, 21 parts -41 parts of fermentation pine needle, 19 parts -37 parts of white thorn and 20 parts -36 parts of sand sagebrush (Artemisia filifolia).

5. according to claim 4 it is a kind of improve camel mammary gland function method for breeding, which is characterized in that the additive by Following parts by weight into being grouped as：65 parts -76 parts of aliphatic acid, 23 parts -34 parts of mango core essential oil, 18 parts -29 parts of glossy privet Seed extract, 23 parts -31 parts of Semen sojae atricolor extract, 21 parts -33 parts of Thallus Laminariae (Thallus Eckloniae) extract and 23 parts -35 parts of analysis for soybean powder.

6. a kind of method for breeding for improving camel mammary gland function according to claim 5, which is characterized in that the aliphatic acid Extracting method includes the following steps：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 part -23 The stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk of part are pre-processed respectively, are then mixed It closes, obtains extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v): 1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, temperature is directly placed into after taking-up It spends in the water bath with thermostatic control for 90 DEG C -100 DEG C and thaws, heats, when the temperature of mixture reaches bath temperature, continue water-bath Heat 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasonic extraction Ultrasonic power density is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；Be ultrasonically treated according to " open ultrasound into Row supersound process 15s- closing ultrasound 5s- opening ultrasounds carry out the processing mode that supersound process 15s " is 1 cycle and are repeated Processing carries out 20-30 circular treatment in total；

(3) mixture after step (2) is ultrasonically treated takes out, and is filtered after being cooled to room temperature, into filtrate according to quality Than for 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in water bath with thermostatic control under 50 DEG C -60 DEG C of temperature condition 30min-40min, it is 1 to be cooled to after room temperature according to volume ratio:1 adds in petroleum ether-ether in the condition that temperature is 50 DEG C -70 DEG C Under carry out extraction 60min-70min, obtain extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1: 8-10 adds in the petroleum ether that percentage by volume is 10% (v/v) -20% (v/v), is placed in temperature as under conditions of 65 DEG C -75 DEG C Constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed After obtain the aliphatic acid.

The preprocess method of step (1) peanut is：By after peanut decladding with mass concentration be 1%-3% NaHCO₃Solution It is 2-4 according to solid-liquid mass ratio:1-2 mix, grinding after drying to moisture content for 3%-5% to obtain the final product.

The preprocess method of step (1) the oil tea slag is：By NaCl solution that oil tea slag and mass concentration are 1%-3% according to Solid-liquid mass ratio is 2-4:1 is mixed, and water-bath 2h-3h filters to take filter residue drying to moisture content under conditions of 60 DEG C -70 DEG C For 3%-5% to obtain the final product.

The preprocess method of step (1) stool in mice is：The stool in mice that mouse moisture content is 2%-4% is pulverized, so It is afterwards 5.5 × 10 with living bacteria count⁹~8.1 × 10⁹CFU/ml bacillus subtilises bacterium solution is 20-30 according to mass ratio:1 into Tunning and mass concentration are 1%-3%'s after fermentation by row mixing, the anaerobic fermentation 15d-20d under conditions of 25-27 DEG C NaCl solution is 3-5 according to mass ratio:1 is mixed, and water-bath 2h-3h filters to take filter residue drying under conditions of 65 DEG C -75 DEG C To moisture content for 3%-5% to obtain the final product.

The preprocess method of step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered and takes filter residue and effective viable bacteria Number is 2.5 × 10⁹~6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 15-20 according to mass ratio:1 is mixed, at 23-25 DEG C Under the conditions of anaerobic fermentation 20d-25d, by NaHCO that tunning and mass concentration are 1%-3% after fermentation₃Solution is according to quality Than for 3-5:1-2 is mixed, and it is 3%- that water-bath 2h-3h, which filters to take filter residue and dries to moisture content, under conditions of 45 DEG C -55 DEG C 5% to obtain the final product.

The preprocess method of step (1) milk is：It is 3.5 × 10 by milk and living bacteria count⁹~6.7 × 10⁹CFU/ml Saccharomycete bacterium solution is 18-23 according to mass ratio:1 is mixed, and ferment 10d-15d under conditions of 26-28 DEG C, will hair after fermentation Ferment product and the NaHCO that mass concentration is 1%-3%₃Solution is 2-6 according to mass ratio:2-3 is mixed, at 45 DEG C -55 DEG C Under conditions of water-bath 1h-2h to obtain the final product.