CN108522786A

CN108522786A - A kind of feed and preparation method thereof improving hunchbacked milk yield

Info

Publication number: CN108522786A
Application number: CN201810141672.2A
Authority: CN
Inventors: 隆杰
Original assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Current assignee: Guigang Nation Agriculture And Animal Husbandry Science And Technology Ltd Co
Priority date: 2018-02-11
Filing date: 2018-02-11
Publication date: 2018-09-14

Abstract

The present invention relates to feed processing technology fields, more particularly to a kind of feed and preparation method thereof improving hunchbacked milk yield, the feed of the application is made of additive, fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, sunflower shell powder and maize cob meal, wherein, additive component is the active ingredient that the camel output of milk can be improved of the application applicant's independent research；Contain abundant protein in napier grass, eucalyptus leaves, cassava, the nervous system of camel can be stimulated after fermentation to greatest extent, make camel eating；Simultaneously, moreover it is possible to discharge the small-molecule substances such as amino acid, the vitamin in greenfeed, the constitution for being suitble to the female camel of just production more weak；Sunflower shell powder in feed and maize cob meal can meet female camel to the primary demand of nutriment, the application feed formula is reasonable, can safely and effectively improve hunchbacked milk yield containing abundant crude protein.

Description

A kind of feed and preparation method thereof improving hunchbacked milk yield

【Technical field】

The present invention relates to feed processing technology field, more particularly to a kind of feed improving hunchbacked milk yield and its preparation side Method.

【Background technology】

The various nutritional ingredients that bactrian camel milk and camel dairy products are needed containing human body are best one of the wholefoods of the mankind, Bactrian camel milk is developed and used to improving household's actions, grows local economy, development characteristic dairy products industry, meeting the market demand has Significance.Since reducing the process and development with camel milk due to domestic camel cultivation quantity, cause the price of camel also continuous It is soaring, to bring new vitality to the development of cultivation camel industry.But it is current camel milk development that the camel output of milk is not high Bottleneck problem in the process.

The output of milk of camel depends primarily on the supply situation of feed and water, improves nutritive value in feed and is produced to improving Milk amount plays a very important role.Feed addictive refer to Feed Manufacturing processing, use during add it is a small amount of or micro- Quantity of material, dosage is seldom in feed but effect is notable.The breeding way of camel, which mostly uses, puts in a suitable place to breed, this is because camel is to feed Taste requirements it is higher, if feed will not be eaten by being unable to effective stimulus camel taste bud camel, during conventional cultivation, camel pair The selection of forage grass be with seasonal variations, and it is selective during feeding, especially like seasonal forage.And more The annual herbage of preference feeding；Since " particular about food " of camel is currently, research to its formula forage not yet, and formula forage With being convenient for carrying, feeding advantage easy to operate, therefore, camel production can also safely, effectively be improved by how developing one kind Milk amount, the camel greenfeed for meeting camel mouthfeel are that camel poultry feeders needs urgently solve the problems, such as.

【Invention content】

In view of the above, it is necessary to provide a kind of camel feed, can also can safely, effectively improve the camel output of milk, together When can also meet camel mouthfeel, improve the palatability of feed.

In order to achieve the above objectives, the technical solution adopted in the present invention is：

A kind of feed improving hunchbacked milk yield, the feed comprise the following components in parts by weight：5 parts -11 parts of addition Agent, 33 parts -44 parts of fermentation napier grass, 27 parts -37 parts of fermentation eucalyptus leaves, 21 parts -41 parts of fermentation cassava, 19 parts -37 parts Sunflower shell powder and 20 parts -36 parts of maize cob meal.

Further, the additive in the application feed comprises the following components in parts by weight：55 parts -63 parts of fat Acid, 17 parts -23 parts of olive oil, 9 parts -13 parts of sunflower seed extract, 18 parts -29 parts of lemon extract, 9 parts -16 parts Banana extract and 17 parts -32 parts of albumen powder.

Further, the preparation method of aliphatic acid includes the following steps in the application additive：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 Part -23 parts of stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk are pre-processed respectively, then into Row mixing obtains extraction material；

(2) by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v) according to mass ratio It is 1:1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, is directly put after taking-up Enter in the water bath with thermostatic control that temperature is 90 DEG C -100 DEG C to thaw, heating, when the temperature of mixture reaches bath temperature, continue Heating water bath 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasound carries The ultrasonic power density taken is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；It is ultrasonically treated according to " opening is super It is that 1 processing mode recycled carries out that sound, which be ultrasonically treated 15s- and close ultrasound 5s- and open ultrasound to carry out supersound process 15s ", Reprocessing carries out 20-30 circular treatment in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in thermostatted water under 50 DEG C -60 DEG C of temperature condition 30min-40min is bathed, it is 1 to be cooled to after room temperature according to volume ratio:1 is added the item that petroleum ether-ether is 50 DEG C -70 DEG C in temperature Extraction 60min-70min is carried out under part, and extract I is obtained after taking ether layer recycling design；It is according to solid-liquid mass ratio into filter residue 1:The petroleum ether that percentage by volume is 10% (v/v) -20% (v/v) is added in 8-10, is placed in the condition that temperature is 65 DEG C -75 DEG C Lower constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed The aliphatic acid is obtained after conjunction.

Further, the preprocess method of step (1) peanut is in the application addictive preparation method：After peanut decladding With the NaHCO that mass concentration is 1%-3%₃Solution is 2-4 according to solid-liquid mass ratio:1-2 is mixed, and is dried to aqueous after grinding Rate be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) oil tea slag is in the application addictive preparation method：By oil tea slag with The NaCl solution that mass concentration is 1%-3% is 2-4 according to solid-liquid mass ratio:1 is mixed, under conditions of 60 DEG C -70 DEG C Water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) stool in mice is in the application addictive preparation method：Mouse is contained Water rate is that the stool in mice of 2%-4% is pulverized, and is then 5.5 × 10 with living bacteria count⁹~8.1 × 10⁹CFU/ml withered grass gemma Bacillus bacterium solution is 20-30 according to mass ratio:1 is mixed, anaerobic fermentation 15d-20d under conditions of 25-27 DEG C, will after fermentation The NaCl solution that tunning is 1%-3% with mass concentration is 3-5 according to mass ratio:1 is mixed, at 65 DEG C -75 DEG C Under the conditions of water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) suspended sludge in milk wastewater is in the application addictive preparation method：It will be newborn It is 2.5 × 10 that product waste water, which is filtered and takes filter residue and living bacteria count,⁹~6.1 × 10⁹CFU/ml lactic acid bacterial liquids are according to mass ratio For 15-20:1 is mixed, anaerobic fermentation 20d-25d under conditions of 23-25 DEG C, by tunning and mass concentration after fermentation For the NaHCO of 1%-3%₃Solution is 3-5 according to mass ratio:1-2 is mixed, water-bath 2h-3h under conditions of 45 DEG C -55 DEG C Filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

Further, the preprocess method of step (1) milk is in the application addictive preparation method：By milk and effectively Viable count is 3.5 × 10⁹~6.7 × 10⁹CFU/ml saccharomycete bacterium solution is 18-23 according to mass ratio:1 is mixed, in 26-28 Ferment 10d-15d under conditions of DEG C, by NaHCO that tunning and mass concentration are 1%-3% after fermentation₃Solution is according to quality Than for 2-6:2-3 is mixed, and water-bath 1h-2h under conditions of 45 DEG C -55 DEG C to obtain the final product.

Further, the preparation method of the application feed is：

(1) napier grass prepares fermentation napier grass：Fresh napier grass is shredded, is then 25-30 according to napier grass and probiotics mass ratio: 1 addition probiotics I ingredients are 23 DEG C -27 DEG C in temperature, and humidity is fermented under conditions of being 15%-25%, and fermentation time is 25d-35d, drying is that 10%-15% obtains the fermentation napier grass to moisture content under the conditions of 50 DEG C -55 DEG C；The probiotics I Ingredient by the living bacteria count after activating be 2.6 × 10⁹~9.6 × 10⁹CFU/g aspergillus oryzaes and living bacteria count be 4.2 × 10⁹~8.5 × 10⁹CFU/g saccharomycete is 2-4 according to mass ratio:1-3 is formed；

(2) eucalyptus leaves prepare fermentation eucalyptus leaves：According to mass ratio it is 25-30 by fresh eucalyptus leaves and spray:1 mixing is cut It is broken, it is then 30-35 according to eucalyptus leaves mixture and probiotics mass ratio:1 addition II ingredient of probiotics, temperature be 25 DEG C- 30 DEG C, humidity is fermented under conditions of being 20%-30%, and fermentation time 30d-40d is dried under the conditions of 60 DEG C -65 DEG C It is that 8%-18% obtains the fermentation eucalyptus leaves to moisture content；The ingredient of the probiotics II is by the living bacteria count after activating 1.9×10⁹~9.2 × 10⁹CFU/g aspergillus oryzaes and living bacteria count are 2.4 × 10⁹~8.5 × 10⁹CFU/g bacillus subtilises It is 5-8 according to mass ratio:2-4 is formed；

(3) cassava prepares fermentation cassava：According to mass ratio it is 1 by cassava skin and cassava stem:3-5 is mixed, and is then pressed It is 20-25 according to cassava mixture and probiotics mass ratio:1 addition III ingredient of probiotics, is 25 DEG C -30 DEG C in temperature, humidity is Ferment under conditions of 22%-32%, fermentation time 28d-38d, under the conditions of 55 DEG C -60 DEG C drying to moisture content be 12%-17% obtains the fermentation cassava；The ingredient of the probiotics III is 2.3 × 10 by the living bacteria count after activating⁹~ 7.4×10⁹CFU/g lactic acid bacterias and living bacteria count are 2.1 × 10⁹~8.3 × 10⁹CFU/g saccharomycete is 2-4 according to mass ratio: 1-3 is formed；

(4) each material composition is weighed by the weight ratio, by major ingredient：Fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, certain herbaceous plants with big flowers Flower shell powder and maize cob meal crush after being thoroughly mixed, and are screened by -100 mesh screen of 30 mesh, finally add addition Agent, drying to moisture content are that 3%-5% obtains the feed.

The present invention has the advantages that：

1, the feed of the application is by additive, fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, sunflower shell powder and corncob Powder forms, wherein additive component is the active ingredient that the camel output of milk can be improved of the application applicant's independent research；As Contain abundant protein in grass, eucalyptus leaves, cassava, and eucalyptus leaves have the ferment fragrance after dense smell and fermentation mixed It closes, can more stimulate camel taste bud, but the smell of eucalyptus leaves is excessively dense, if the wine to ferment with the fragrant of napier grass and cassava Fragrance mixes, and will stimulate the nervous system of camel to greatest extent, make camel eating；And by napier grass, eucalyptus leaves, wood in feed Potato after being fermented the small-molecule substances such as amino acid therein, vitamin adequately released, be suitble to the female white horse with a black mane just produced Hunchbacked constitution；Also contain sunflower shell powder and maize cob meal in the feed of the application, these ingredients contain abundant crude protein, can meet Primary demand of female camel to nutriment.

2, used probiotics all have passed through activation during the fermentation for the napier grass of the application, eucalyptus leaves, cassava, and activation is trained Support it is different degrees of in base add napier grass juice, eucalyptus leaf juice, cassava juice etc., these ingredients can be probiotic enriched, accelerates prebiotic Bacterium to napier grass, eucalyptus leaves, cassava fermentation.

3, the feed addictive of the application is extracted by aliphatic acid, olive oil, sunflower seed extract, lemon extract, banana Object and albumen powder form, and in ingredient, aliphatic acid is the aliphatic acid that applicant independently extracts, wherein containing the unsaturation compared with horn of plenty Aliphatic acid, unsaturated fatty acid Linoleic acid content is 69.47% or so, and oleic acid content is 22.02% or so；In olive oil Containing abundant unsaturated fat sour component, abundant sunflower oil is contained in sunflower seed extract, is contained in sunflower oil rich Rich linoleic acid, these unsaturated fatty acids can effectively remove camel interior free yl, can improve the immunity of camel, effectively drop Low camel hyperlipidemia, blood pressure happen, and can remove thrombus dredging mammary gland, reach the mesh for the mammary gland function for improving camel mammary gland , in the application, abundant vitamin C is contained in lemon extract, contains abundant general flavone, dimension life in banana extract The ingredients such as plain C, polysaccharide, protein, vitamin is mainly joined in the form of coenzyme and catalyst extensively in body in these ingredients With the synthesis and degradation of nutrient, to ensure the eucaryotic cell structure of histoorgan and the normal operation of function, to maintain animal strong Health and various production activities.Being deficient in vitamin can cause animal metabolism disorderly, influence animal health and production performance.In daily ration The production performance and milk quality of ruminant can be improved by adding these ingredients, and strengthen immunity improves its health status, to carry The high output of milk.

【Specific implementation mode】

All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.

Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each Feature is an example in a series of equivalent or similar characteristics.

Embodiment 1：

A kind of feed improving hunchbacked milk yield is present embodiments provided, the feed comprises the following components in parts by weight：5 The additive, 33 parts of fermentation napier grass, 27 parts of fermentation eucalyptus leaves, 21 parts of fermentation cassava, 19 parts of sunflower shell powder and 20 of part The maize cob meal of part.

The additive of the present embodiment comprises the following components in parts by weight：55 parts of aliphatic acid, 17 parts of olive oil, 9 parts Sunflower seed extract, 18 parts of lemon extract, 9 parts of banana extract and 17 parts of albumen powder.

The extracting method of above-mentioned sunflower seed extract is：The sunflower seeds that moisture content is 3% is smashed to pieces, then passes through 70 mesh and sieves Net is screened, and sunflower seeds dry powder is obtained, and dry powder is put into extraction kettle, when pressure is 15MPa, with liquid CO₂Static state extraction 15min is taken, is then gradually released stress, supercritical CO is carried out₂Dynamic extraction, dynamic extraction technological parameter are：When temperature heats It when to 55 DEG C, pressurizes to system, after pressure reaches 25MPa, adjusts CO₂Flow is 6L/min, and constant temperature and pressure is kept to carry out It discharges after cycling extraction 2h, 1min is centrifuged under conditions of 500r/min, obtain sunflower seed extract, sunflower seed extract In sunflower oil content reach 96.77%

The extracting method of above-mentioned lemon extract is：By the acetone soln of chilli seed and 100mg/g that moisture content is 4% It is 1 according to solid-liquid mass ratio:3 are mixed, and are soaked 5 times, each 1h in 60 DEG C of water-bath medium temperatures, filtering, merging filtrate depressurizes back Acetone is received, until paste, the relative density of thick paste object is 1.07g/cm³Obtain lemon extract, it is ascorbic in extract to contain Amount is 97.33mg/g.

The extracting method of above-mentioned banana extract is：Banana peeling after smash to pieces afterwards with percentage by volume be 75% ethyl alcohol by It is 1 according to mass ratio:2 are mixed, and refluxing extraction is then carried out in refluxing extraction device, and Extracting temperature is 90 DEG C, extraction time For 2h, acetone is recovered under reduced pressure, until paste, the relative density of thick paste object is 1.21g/cm³Banana extract is obtained, in extract General flavone content is 102.33mg/g, Vitamin C content 87.64mg/g, polyoses content 212.85mg/g.

The soya bean protein content of above-mentioned albumen powder is 63%；Protein content of whey is 33%.

The preparation method of aliphatic acid includes the following steps in above-mentioned additive：

(1) it is by parts by weight：25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts of stool in mice, 9 parts Suspended sludge in milk wastewater and 9 parts of milk pre-processed respectively, then mixed, obtain extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -20 DEG C and suddenly freezes 30min, water bath with thermostatic control at a temperature of 90 °C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 10min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 400w/L；It is ultrasonically treated Temperature is 130 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 20 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 1:The HCl solution that 1 addition mass concentration is 2% is placed in water bath with thermostatic control 30min under 50 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 60min- under conditions of temperature is 50 DEG C 70min obtains extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:8, which are added percentage by volumes, is The petroleum ether of 10% (v/v) is placed in constant temperature under conditions of temperature is 65 DEG C and extracts 70min, obtained after taking ether layer recycling design Extract II；Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of step (1) peanut is in the present embodiment addictive preparation method：By after peanut decladding with quality A concentration of 1% NaHCO3 solution is 2 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content be 3% to obtain the final product.

The preprocess method of step (1) oil tea slag is in the present embodiment addictive preparation method：Oil tea slag and quality is dense The NaCl solution that degree is 1% is 2 according to solid-liquid mass ratio:1 is mixed, and water-bath 2h filters to take filter residue baking under conditions of 60 DEG C It is 3% to do moisture content to obtain the final product.

The preprocess method of step (1) stool in mice is in the present embodiment addictive preparation method：It is by mouse moisture content 2% stool in mice is pulverized, and is then 5.5 × 10 with living bacteria count⁹CFU/ml bacillus subtilis bacterium solutions are according to mass ratio It is 20:1 is mixed, anaerobic fermentation 15d under conditions of 25 DEG C, by tunning and mass concentration is 1% after fermentation NaCl solution is 3 according to mass ratio:1 is mixed, and water-bath 2h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C is 3% to obtain the final product.

The preprocess method of step (1) suspended sludge in milk wastewater is in the present embodiment addictive preparation method：By Dairy Wastewater It is 2.5 × 10 to be filtered and take filter residue and living bacteria count⁹CFU/ml lactic acid bacterial liquids are 15 according to mass ratio:1 is mixed, Anaerobic fermentation 20d-25d under conditions of 23 DEG C, the NaHCO for being 1% by tunning and mass concentration after fermentation₃Solution according to Mass ratio is 3:1 is mixed, and it is 3%- that water-bath 2h-3h, which filters to take filter residue and dries to moisture content, under conditions of 45 DEG C -55 DEG C 5% to obtain the final product.

The preprocess method of step (1) milk is in the present embodiment addictive preparation method：By milk and living bacteria count It is 3.5 × 10⁹CFU/ml saccharomycete bacterium solution is 18 according to mass ratio:1 is mixed, and ferment 10d under conditions of 26 DEG C, fermentation The NaHCO for being afterwards 1% by tunning and mass concentration₃Solution is 2 according to mass ratio:2 are mixed, in 45 DEG C DEG C of condition Lower water-bath 1h to obtain the final product.

The preparation method of the present embodiment additive is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and olive oil:10 be sufficiently mixed after 2000r/min stir It is stirred to obtain emulsion under the action of machine；

(3) sunflower seed extract, lemon extract, banana extract and albumen powder are added into the emulsion of step (2), The tapioca starch that mixture quality 1/4 is added after being sufficiently stirred, it is 2.02g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The preparation method of the present embodiment feed is：

(1) napier grass prepares fermentation napier grass：Fresh napier grass is shredded, is then 25 according to napier grass and probiotics mass ratio:1 adds Add probiotics I ingredients, is 23 DEG C in temperature, humidity is fermented under conditions of being 15%, fermentation time 25d, in 50 DEG C of items Drying obtains the fermentation napier grass to moisture content for 10% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 2.6 × 10⁹CFU/g aspergillus oryzaes and living bacteria count are 4.2 × 10⁹CFU/g saccharomycete is 2 according to mass ratio:1 composition；

(2) eucalyptus leaves prepare fermentation eucalyptus leaves：According to mass ratio it is 25 by fresh eucalyptus leaves and spray:1 mixing shreds, so It is afterwards 30 according to eucalyptus leaves mixture and probiotics mass ratio:1 addition II ingredient of probiotics, is 25 DEG C in temperature, humidity is Ferment under conditions of 20%-30%, fermentation time 30d, under the conditions of 60 DEG C drying to moisture content be 8% obtain described in Ferment eucalyptus leaves；The ingredient of the probiotics II is 1.9 × 10 by the living bacteria count after activating⁹CFU/g aspergillus oryzaes and effectively Viable count is 2.4 × 10⁹CFU/g bacillus subtilises are 5 according to mass ratio:2 compositions；

(3) cassava prepares fermentation cassava：According to mass ratio it is 1 by cassava skin and cassava stem:3 are mixed, then according to Cassava mixture is 20 with probiotics mass ratio:1 addition III ingredient of probiotics is 25 DEG C in temperature, the condition that humidity is 22% Under ferment, fermentation time 28d, under the conditions of 55 DEG C drying obtain the fermentation cassava to moisture content for 12%；It is described The ingredient of probiotics III is 2.3 × 10 by the living bacteria count after activating⁹CFU/g lactic acid bacterias and living bacteria count be 2.1 × 10⁹CFU/g saccharomycete is 2 according to mass ratio:1 composition；

(4) each material composition is weighed by the weight ratio, by major ingredient：Fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, certain herbaceous plants with big flowers Flower shell powder and maize cob meal crush after being thoroughly mixed, and are screened by 30 mesh screens, finally add additive, dry To moisture content the feed is obtained for 3%.

The formula of the present embodiment probiotics I activation mediums is (total volume 1L)：Mass concentration is the fiber of 18mg/mL Element, mass concentration be 10mg/mL banana polysaccharide, mass concentration be 20mg/mL beef extract, mass concentration be 15mg/mL's Sodium alginate that agar that peptone, mass concentration are 10mg/mL, mass concentration are 5mg/mL, remaining be napier grass juice；Wherein, The processing method of napier grass juice is：According to mass ratio it is 40 by cellulase and water that napier grass, enzyme activity intensity are 500U/g:1:80 into Row mixing, is put into 37 DEG C of insulating boxs and reacts 3d, and filtering removal filtrate is made.

The formula of II activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 22mg/mL It is 20mg/mL's to tie up beef extract, mass concentration that element, the chitosan that mass concentration is 15mg/mL, mass concentration are 18mg/mL Agar that dragon fruit polysaccharide that yeast extract, mass concentration are 5mg/mL, mass concentration are 15mg/mL, remaining be eucalyptus leaf juice；Its In, the processing method of eucalyptus leaf juice is：It is according to mass ratio by cellulase and water that eucalyptus leaves, enzyme activity intensity are 500U/g 35:2:100 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

The formula of III activation medium of the present embodiment probiotics is (total volume 1L)：Mass concentration is the fibre of 25mg/mL Dimension element, mass concentration be 5mg/mL sodium chloride, mass concentration be 20mg/mL beef extract, mass concentration be 20mg/mL ferment Agar that the glucose of peptone, 12mg/mL that female cream, mass concentration are 15mg/mL, mass concentration are 15mg/mL, remaining be Cassava juice；Wherein, the processing method of cassava juice is：By cassava skin, cassava stem, the cellulase and water that enzyme activity intensity is 500U/g It is 10 according to mass ratio：30:1:100 are mixed, and are put into 37 DEG C of insulating boxs and are reacted 3d, and filtering removal filtrate is made.

Embodiment 2：

Present embodiments provide a kind of feed improving hunchbacked milk yield, a kind of feed improving hunchbacked milk yield, the feed It comprises the following components in parts by weight：11 parts of additive, 44 parts of fermentation napier grass, 37 parts of fermentation eucalyptus leaves, 41 parts of hair Ferment cassava, 37 parts of sunflower shell powder and 36 parts of maize cob meal.

The additive of the present embodiment comprises the following components in parts by weight：63 parts of aliphatic acid, 23 parts of olive oil, 13 parts Sunflower seed extract, 29 parts of lemon extract, 16 parts of banana extract and 32 parts of albumen powder.

The present embodiment sunflower seed extract, lemon extract, the extracting method of banana extract and embodiment 1 complete one It causes；And the soya bean protein of albumen powder, protein content of whey and embodiment 1 are completely the same.

(1) it is by parts by weight：46 parts of peanut, 34 parts of oil tea slag, 34 parts of wintersweet seed, 23 parts of stool in mice, 17 The suspended sludge in milk wastewater and 15 parts of milk of part are pre-processed respectively, are then mixed, and extraction material is obtained；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 90% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -15 DEG C and suddenly freezes 50min, and the water bath with thermostatic control that temperature is 100 DEG C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 15min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 700w/L；It is ultrasonically treated Temperature is 150 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 30 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 3:The HCl solution that 1 addition mass concentration is 5% is placed in water bath with thermostatic control 40min under 60 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 70min under conditions of temperature is 70 DEG C, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:10 are added percentage by volume as 20% (v/v) Petroleum ether, be placed in temperature be 75 DEG C under conditions of constant temperature extract 80min, obtain extract II after taking ether layer recycling design； Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of the present embodiment step (1) peanut is：By the NaHCO for being 3% with mass concentration after peanut decladding₃ Solution is 4 according to solid-liquid mass ratio:2 mixing, after grinding drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：The NaCl solution for being 3% by oil tea slag and mass concentration It is 4 according to solid-liquid mass ratio:1 is mixed, under conditions of 70 DEG C water-bath 3h filter to take filter residue drying to moisture content be 5% i.e. .

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is % is pulverized, Then it is 8.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 30 according to mass ratio:1 is mixed, 27 NaCl solution that tunning and mass concentration are 3% according to mass ratio is 5 after fermentation by anaerobic fermentation 20d under conditions of DEG C: 1 is mixed, under conditions of 75 DEG C water-bath 3h filter to take filter residue drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 6.1 × 10⁹CFU/ml lactic acid bacterial liquids are 20 according to mass ratio:1 is mixed, and is detested under conditions of 25 DEG C Aerobe fermentation 20d-25d, the NaHCO for being 3% by tunning and mass concentration after fermentation₃Solution is 5 according to mass ratio:2 carry out Mixing, under conditions of 45 DEG C -55 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 5% to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 6.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 23 according to mass ratio:1 is mixed, and ferment 15d under conditions of 28 DEG C, by tunning and matter after fermentation Measure a concentration of 3% NaHCO₃Solution is 6 according to mass ratio:3 are mixed, and water-bath 2h under conditions of 55 DEG C to obtain the final product.

The preparation method of the present embodiment additive is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and olive oil:15 be sufficiently mixed after 2500r/min stir It is stirred to obtain emulsion under the action of machine；

(3) sunflower seed extract, lemon extract, banana extract and albumen powder are added into the emulsion of step (2), The tapioca starch that mixture quality 1/2 is added after being sufficiently stirred, it is 3.14g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The preparation method of the present embodiment feed is：

(1) napier grass prepares fermentation napier grass：Fresh napier grass is shredded, is then 30 according to napier grass and probiotics mass ratio:1 adds Add probiotics I ingredients, is 27 DEG C in temperature, humidity is fermented under conditions of being 15%-25%, fermentation time 35d, 55 Drying obtains the fermentation napier grass to moisture content for 15% under the conditions of DEG C；The ingredient of the probiotics I is by effective work after activating Bacterium number is 9.6 × 10⁹CFU/g aspergillus oryzaes and living bacteria count are 8.5 × 10⁹CFU/g saccharomycete is 4 according to mass ratio:3 compositions；

(2) eucalyptus leaves prepare fermentation eucalyptus leaves：According to mass ratio it is 30 by fresh eucalyptus leaves and spray:1 mixing shreds, so It is afterwards 35 according to eucalyptus leaves mixture and probiotics mass ratio:1 addition II ingredient of probiotics, is 30 DEG C in temperature, humidity is It ferments under conditions of 20%-30%, fermentation time 40d is dried to moisture content under the conditions of 65 DEG C and obtained institute for 18% State fermentation eucalyptus leaves；The ingredient of the probiotics II is 9.2 × 10 by the living bacteria count after activating⁹CFU/g aspergillus oryzaes and have It is 8.5 × 10 to imitate viable count⁹CFU/g bacillus subtilises are 8 according to mass ratio:4 compositions；

(3) cassava prepares fermentation cassava：According to mass ratio it is 1 by cassava skin and cassava stem:5 are mixed, then according to Cassava mixture is 25 with probiotics mass ratio:1 addition III ingredient of probiotics is 30 DEG C in temperature, the condition that humidity is 32% Under ferment, fermentation time 38d, under the conditions of 60 DEG C drying obtain the fermentation cassava to moisture content for 17%；It is described The ingredient of probiotics III is 7.4 × 10 by the living bacteria count after activating⁹CFU/g lactic acid bacterias and living bacteria count be 8.3 × 10⁹CFU/g saccharomycete is 4 according to mass ratio:3 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, certain herbaceous plants with big flowers Flower shell powder and maize cob meal crush after being thoroughly mixed, and are screened by 100 mesh screens, finally add additive, dry It does moisture content and obtains the feed for 5%.

The formula and embodiment 1 of III activation medium of the present embodiment probiotics I, probiotics II and probiotics are completely the same.

Embodiment 3：

Present embodiments provide a kind of feed improving hunchbacked milk yield, a kind of feed improving hunchbacked milk yield, the feed It comprises the following components in parts by weight：7 parts of additive, 38 parts of fermentation napier grass, 32 parts of fermentation eucalyptus leaves, 30 parts of fermentation Cassava, 23 parts of sunflower shell powder and 25 parts of maize cob meal.

The additive of the present embodiment comprises the following components in parts by weight：60 parts of aliphatic acid, 20 parts of olive oil, 12 parts of sunflower seed extract, 21 parts of lemon extract, 12 parts of banana extract and 21 parts of albumen powder.

(1) it is by parts by weight：36 parts of peanut, 24 parts of oil tea slag, 27 parts of wintersweet seed, 15 parts of stool in mice, 12 The suspended sludge in milk wastewater and 13 parts of milk of part are pre-processed respectively, are then mixed, and extraction material is obtained；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 80% (v/v):1 carries out Mixing is then placed in the refrigerator-freezer that temperature is -17 DEG C and suddenly freezes 40min, and the water bath with thermostatic control that temperature is 95 DEG C is directly placed into after taking-up Middle defrosting, heating continue heating water bath 12min when the temperature of mixture reaches bath temperature；It will be mixed after heating water bath Conjunction object, which is put into supersonic extractors, carries out ultrasonic extraction, and the ultrasonic power density of the ultrasonic extraction is 500w/L；It is ultrasonically treated Temperature is 140 DEG C；It is ultrasonically treated according to " opening ultrasound carries out being ultrasonically treated 15s- closing ultrasound 5s- opening ultrasound progress ultrasounds Processing 15s " is that the processing mode of 1 cycle is reprocessed, and carries out 25 circular treatments in total；

(3) by step (2) be ultrasonically treated after mixture take out, be filtered after being cooled to room temperature, into filtrate according to Mass ratio is 2:The HCl solution that 1 addition mass concentration is 3% is placed in water bath with thermostatic control 35min under 55 DEG C of temperature condition, cooling After to room temperature according to volume ratio be 1:1 addition petroleum ether-ether carries out extraction 65min under conditions of temperature is 60 DEG C, takes ether Extract I is obtained after layer recycling design；Into filter residue according to solid-liquid mass ratio be 1:9 are added percentage by volume as 15% (v/v) Petroleum ether, be placed in temperature be 70 DEG C under conditions of constant temperature extract 75min, obtain extract II after taking ether layer recycling design； Extract I and extract II are obtained into the aliphatic acid after evenly mixing.

The preprocess method of the present embodiment step (1) peanut is：By the NaHCO for being 2% with mass concentration after peanut decladding₃ Solution is 3 according to solid-liquid mass ratio:1 mixing, after grinding drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) oil tea slag is：The NaCl solution for being 2% by oil tea slag and mass concentration It is 3 according to solid-liquid mass ratio:1 is mixed, and it is 4% that water-bath 2.5h, which filters to take filter residue and dries to moisture content, under conditions of 65 DEG C To obtain the final product.

The preprocess method of the present embodiment step (1) stool in mice is：The stool in mice that mouse moisture content is 3% is ground It is broken, it is then 6.1 × 10 with living bacteria count⁹CFU/ml bacillus subtilises bacterium solution is 25 according to mass ratio:1 is mixed, Anaerobic fermentation 18d under conditions of 26 DEG C, after fermentation by tunning and mass concentration be 2% NaCl solution according to mass ratio It is 4:1 is mixed, under conditions of 70 DEG C water-bath 2.5h filter to take filter residue drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) suspended sludge in milk wastewater is：Dairy Wastewater is filtered take filter residue with Living bacteria count is 4.1 × 10⁹CFU/ml lactic acid bacterial liquids are 17 according to mass ratio:1 is mixed, and is detested under conditions of 24 DEG C Aerobe fermentation 22d, the NaHCO for being 2% by tunning and mass concentration after fermentation₃Solution is 4 according to mass ratio:1 is mixed, Under conditions of 50 DEG C water-bath 2.5h filter to take filter residue drying to moisture content be 4% to obtain the final product.

The preprocess method of the present embodiment step (1) milk is：It is 5.7 × 10 by milk and living bacteria count⁹CFU/ml Saccharomycete bacterium solution is 20 according to mass ratio:1 is mixed, and ferment 12d under conditions of 27 DEG C, by tunning and matter after fermentation Measure a concentration of 2% NaHCO₃Solution is 3 according to mass ratio:2 are mixed, and water-bath 1.5h under conditions of 50 DEG C to obtain the final product.

The preparation method of the present embodiment additive is：

(1) each raw material is weighed by the parts by weight；

(2) it is 1 according to mass ratio with water by aliphatic acid and olive oil:12 be sufficiently mixed after 2300r/min stir It is stirred to obtain emulsion under the action of machine；

(3) sunflower seed extract, lemon extract, banana extract and albumen powder are added into the emulsion of step (2), The tapioca starch that mixture quality 1/3 is added after being sufficiently stirred, it is 2.44g/cm that relative density, which is made,³Medicinal extract obtain described add Add agent.

The preparation method of the present embodiment feed is：

(1) napier grass prepares fermentation napier grass：Fresh napier grass is shredded, is then 27 according to napier grass and probiotics mass ratio:1 adds Add probiotics I ingredients, is 25 DEG C in temperature, humidity is fermented under conditions of being 20%, fermentation time 30d, in 52 DEG C of items Drying obtains the fermentation napier grass to moisture content for 12% under part；The ingredient of the probiotics I is by the living bacteria count after activating It is 4.6 × 10⁹CFU/g aspergillus oryzaes and living bacteria count are 6.5 × 10⁹CFU/g saccharomycete is 3 according to mass ratio:2 compositions；

(2) eucalyptus leaves prepare fermentation eucalyptus leaves：According to mass ratio it is 28 by fresh eucalyptus leaves and spray:1 mixing shreds, so It is afterwards 33 according to eucalyptus leaves mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 25 DEG C -30 DEG C in temperature, humidity To ferment under conditions of 20%-30%, fermentation time 35d is dried to moisture content under the conditions of 62 DEG C and is obtained for 12% The fermentation eucalyptus leaves；The ingredient of the probiotics II is 5.2 × 10 by the living bacteria count after activating⁹CFU/g aspergillus oryzaes and Living bacteria count is 4.5 × 10⁹CFU/g bacillus subtilises are 7 according to mass ratio:3 compositions；

(3) cassava prepares fermentation cassava：According to mass ratio it is 1 by cassava skin and cassava stem:4 are mixed, then according to Cassava mixture is 23 with probiotics mass ratio:1 addition III ingredient of probiotics is 27 DEG C in temperature, the condition that humidity is 27% Under ferment, fermentation time 32d, under the conditions of 57 DEG C drying obtain the fermentation cassava to moisture content for 15%；It is described The ingredient of probiotics III is 5.4 × 10 by the living bacteria count after activating⁹CFU/g lactic acid bacterias and living bacteria count be 6.3 × 10⁹CFU/g saccharomycete is 3 according to mass ratio:2 compositions；

(4) each material composition is weighed by the weight ratio, by major ingredient：Fermentation napier grass, fermentation eucalyptus leaves, fermentation cassava, certain herbaceous plants with big flowers Flower shell powder and maize cob meal crush after being thoroughly mixed, and are screened by 60 mesh screens, finally add additive, dry To moisture content the feed is obtained for 4%.

Control group 1：

This control group is not to each raw material：Peanut, oil tea slag, wintersweet seed, stool in mice, suspended sludge in milk wastewater and milk carry out Pretreatment, but directly raw material is mixed and is produced, other extracting methods are identical with embodiment 1.

Control group 2：

This control group raw material does not use peanut, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 parts Stool in mice, 9 parts of suspended sludge in milk wastewater and 9 parts of milk mix after being pre-processed respectively, other extracting methods and implementation Example 1 is identical.

Control group 3：

This control group raw material does not use wintersweet seed, i.e., by 12 parts of oil tea slag, 9 parts of stool in mice, 9 parts of Dairy Wastewater Dross and 9 parts of milk mix after being pre-processed respectively, and other extracting methods are identical with embodiment 1.

Control group 4：

This control group raw material does not use stool in mice, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet seed, 9 The suspended sludge in milk wastewater and 9 parts of milk of part mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 5：

This control group raw material does not use suspended sludge in milk wastewater, i.e., by 25 parts of peanut, 12 parts of oil tea slag, 21 parts of wintersweet Seed, 9 parts of stool in mice and 9 parts of milk mix after being pre-processed respectively, other extracting methods and 1 complete phase of embodiment Together.

Control group 6：

This control group raw material does not use milk, i.e., by 25 parts of peanut, 21 parts of wintersweet seed, 9 parts of stool in mice and 9 parts Suspended sludge in milk wastewater pre-processed respectively after mix, other extracting methods are identical with embodiment 1.

Test experiments 1：

It measures in embodiment 1-3 and control group 1-6, aliphatic acid recovery rate, is specifically shown in Table 1 and GC-MS analysis 1 Hes of embodiment Fatty acid component in control group 1-6, is specifically shown in Table 2：

Table 1

Group	Aliphatic acid recovery rate (%)
		Embodiment 1	57.43
Embodiment 2	58.54
		Embodiment 3	56.34
Control group 1	33.23
		Control group 2	48.76
Control group 3	49,87
		Control group 4	49.76
Control group 5	50.21
		Control group 6	51.24

As seen from the above table, the aliphatic acid recovery rate of embodiment 1-3 is apparently higher than control group 1, and the aliphatic acid of control group 2-6 Recovery rate illustrates to effectively improve using the preprocessing method of raw materials of the application also above control group 1, slightly below embodiment 1-3 The recovery rate of aliphatic acid；Meanwhile it all raw materials of the application being compounded can improve aliphatic acid recovery rate.

2 unit of table：

As seen from the above table, unsaturated fatty acid can be significantly improved using the present processes extraction aliphatic acid：Linoleic acid and The content of oleic acid；And the unsaturated fatty acid of control group 1-6：The content of linoleic plus oleic acid is significantly lower than embodiment 1, illustrates profit Unsaturated fatty acid content can be improved by being extracted aliphatic acid with the cooperation of the raw material of the application and being pre-processed to raw material.

Test experiments 2：(influence of the fatty acid component to the output of milk in additive)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group, carries out statistics, test in one month by a definite date, and calculates the average milk production of camel, is specifically shown in Table 3：

Test group 1：The camel greenfeed of additive+90% prepared by 10% embodiment 1；

Test group 2：The camel greenfeed of additive+90% prepared by 10% control group 1；

Test group 3：The camel greenfeed of additive+90% prepared by 10% control group 2；

Test group 4：The camel greenfeed of additive+90% prepared by 10% control group 3；

Test group 5：The camel greenfeed of additive+90% prepared by 10% control group 4；

Test group 6：The camel greenfeed of additive+90% prepared by 10% control group 5；

Test group 7：The camel greenfeed of additive+90% prepared by 10% control group 6；

Control group：Only feeding camel greenfeed；

Table 3

Group	Average milk production (kg/ heads/day)
		Test group 1	2.86
Test group 2	2.03
		Test group 3	2.11
Test group 4	2.01
		Test group 5	1.98
Test group 6	1.96
		Test group 7	2.14
Test group 8	1.64

As seen from the above table, the output of milk of test group 1-7 is apparently higher than control group, illustrates the additive aliphatic acid of the application Active ingredient forms the output of milk that can effectively improve camel；And the output of milk of test group 1 is higher than test group 2-7, illustrates the application Additive prepare saturated fatty acid and its at be grouped as be improve the camel output of milk most critical ingredient.

Test experiments 3：(influence of the additive formulations to the output of milk)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (test group 1-7) and control Group, carries out statistics, test in one month by a definite date, and calculates the average milk production of camel, is specifically shown in Table 4：

Experiment periods feed formula：

Test group A：The camel greenfeed of 10% additive+90%；Wherein, additive by following parts by weight at grouping At：55 parts of aliphatic acid, 17 parts of olive oil, 9 parts of sunflower seed extract, 18 parts of lemon extract, the extraction of 9 parts of banana Object and 17 parts of albumen powder.(i.e. the additive of embodiment 1).

Experiment group B：The camel greenfeed of 10% additive+90%；Wherein, additive does not add aliphatic acid, i.e.,：17 parts Olive oil, 9 parts of sunflower seed extract, 18 parts of lemon extract, 9 parts of banana extract and 17 parts of albumen powder；Its The preparation method of his additive and embodiment 1 are completely the same.

Test group C：The camel greenfeed of 10% additive+90%；Wherein, additive does not add olive oil, i.e.,：55 parts Aliphatic acid, 9 parts of sunflower seed extract, 18 parts of lemon extract, 9 parts of banana extract and 17 parts of albumen powder；Its The preparation method of his additive and embodiment 1 are completely the same.

Test group D：The camel greenfeed of 10% additive+90%；Wherein, additive does not add sunflower seed extract, I.e.：55 parts of aliphatic acid, 17 parts of olive oil, 18 parts of lemon extract, 9 parts of banana extract and 17 parts of albumen powder； The preparation method of other additives and embodiment 1 are completely the same.

Test group E：The camel greenfeed of 10% additive+90%；Wherein, additive does not add lemon extract, i.e.,： 55 parts of aliphatic acid, 17 parts of olive oil, 9 parts of sunflower seed extract, 9 parts of banana extract and 17 parts of albumen powder；Its The preparation method of his additive and embodiment 1 are completely the same.

Test group F：The camel greenfeed of 10% additive+90%；Wherein, additive does not add banana extract, i.e.,： 55 parts of aliphatic acid, 17 parts of olive oil, 9 parts of sunflower seed extract, 18 parts of lemon extract and 17 parts of albumen powder；Its The preparation method of his additive and embodiment 1 are completely the same.

Test group G：The camel greenfeed of 10% additive+90%；Wherein, additive does not add albumen powder, i.e.,：55 parts Aliphatic acid, 17 parts of olive oil, 9 parts of sunflower seed extract, 18 parts of lemon extract and 9 parts of banana extract；Its The preparation method of his additive and embodiment 1 are completely the same.

Control group：Only feeding camel greenfeed.

Table 4

Group	Average milk production (kg/ heads/day)
		Test group A	2.86
Experiment group B	1.97
		Test group C	2.03
Test group D	2.11
		Test group E	2.15
Test group F	2.03
		Test group G	2.07
Control group	1.64

As seen from the above table, the output of milk of test group A is higher than experiment group B-G control groups, illustrates the additive formulations of the application The output of milk of camel can be effectively improved, and additive component is indispensable, the output of milk of test group C-G is higher than experiment group B, examination Test group A the output of milk be apparently higher than experiment group B illustrate in additive component mainly to serve improve the output of milk the application it is made Standby aliphatic acid.

Testing experiment 4：(influence of the feed formula to the output of milk)

80 milk production phase camels are divided into 8 groups, every group by following feed formula feeding camel (experimental group 1-7) and control Group, carries out statistics, test in one month by a definite date, and calculates the average milk production of camel, and record the results are shown in Table 5：

Experiment periods feed formula：

Experimental group 1：The feed comprises the following components in parts by weight：5 parts of additive, 33 parts of fermentation napier grass, 27 The fermentation eucalyptus leaves, 21 parts of fermentation cassava, 19 parts of sunflower shell powder and 20 parts of maize cob meal of part.(the i.e. feed of embodiment 1 Formula).

Experimental group 2：The feed comprises the following components in parts by weight：5 parts of additive, 27 parts of fermentation eucalyptus leaves, 21 parts of fermentation cassava, 19 parts of sunflower shell powder and 20 parts of maize cob meal.

Experimental group 3：The feed comprises the following components in parts by weight：5 parts of additive, 33 parts of fermentation napier grass, 21 The fermentation cassava, 19 parts of sunflower shell powder and 20 parts of maize cob meal of part.

Experimental group 4：The feed comprises the following components in parts by weight：5 parts of additive, 33 parts of fermentation napier grass, 27 The fermentation eucalyptus leaves, 19 parts of sunflower shell powder and 20 parts of maize cob meal of part.

Experimental group 5：The feed comprises the following components in parts by weight：5 parts of additive, 33 parts of fermentation napier grass, 27 The fermentation eucalyptus leaves, 21 parts of fermentation cassava and 20 parts of maize cob meal of part.

Experimental group 6：The feed comprises the following components in parts by weight：5 parts of additive, 33 parts of fermentation napier grass, 27 The fermentation eucalyptus leaves, 21 parts of fermentation cassava and 19 parts of sunflower shell powder of part.

Experimental group 7：The feed comprises the following components in parts by weight：33 parts of fermentation napier grass, 27 parts of fermentation eucalyptus Leaf, 21 parts of fermentation cassava, 19 parts of sunflower shell powder and 20 parts of maize cob meal.

Control group：The feed comprises the following components in parts by weight：19 parts of sunflower shell powder and 20 parts of maize cob meal.

Table 5

As seen from the above table, the output of milk of experimental group 1 is more than experimental group 2-7 and control group, even higher than test group 1 in table 3 The output of milk illustrate that the feed formula of the application can effectively improve the output of milk of camel；The output of milk of experimental group 1 is more than experimental group The output of milk of 2-7 illustrates that 1 is lacked in the application feed formula can all influence the output of milk of camel；Wherein, the output of milk of experimental group 1 Significantly greater than experimental group 7；The output of milk of experimental group 2-6 is more than experimental group 7, illustrates to play main raising in the application feed formula The active ingredient of the output of milk is additive.

In conclusion the output of milk of camel can be effectively improved using the feed formula of the present invention, and ingredient is indispensable, The feed of the application, which is cobalt blue pigment, additive formulations are taken from plant source will not cause feed residual and additive residual, be one Kind safely, effectively, the feed of hunchbacked milk yield can be improved.

Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of feed improving hunchbacked milk yield, which is characterized in that the feed comprises the following components in parts by weight：5 part -11 Part additive, 33 parts -44 parts of fermentation napier grass, 27 parts -37 parts of fermentation eucalyptus leaves, 21 parts -41 parts of fermentation cassava, 19 - 37 parts of sunflower shell powder of part and 20 parts -36 parts of maize cob meal.

2. the feed according to claim 1 for improving hunchbacked milk yield, which is characterized in that the additive is by following parts by weight At being grouped as：55 parts -63 parts of aliphatic acid, 17 parts -23 parts of olive oil, 9 parts -13 parts of sunflower seed extract, 18 part -29 The lemon extract, 9 parts -16 parts of banana extract and 17 parts -32 parts of albumen powder of part.

3. the feed according to claim 2 for improving hunchbacked milk yield, which is characterized in that the extracting method packet of the aliphatic acid Include following steps：

(1) it is by parts by weight：25 parts -46 parts of peanut, 12 parts -34 parts of oil tea slag, 21 parts -34 parts of wintersweet seed, 9 part -23 The stool in mice, 9 parts -17 parts of suspended sludge in milk wastewater and 9 parts -15 parts of milk of part are pre-processed respectively, are then mixed It closes, obtains extraction material；

(2) it is 1 according to mass ratio by ethyl alcohol that the extraction material of step (1) and percentage by volume are 75% (v/v) -90% (v/v): 1 is mixed, and is then placed in the refrigerator-freezer that temperature is -20 DEG C~-15 DEG C and is suddenly frozen 30min-50min, temperature is directly placed into after taking-up Degree, when the temperature of mixture reaches bath temperature, continues water-bath to thaw, heating in 90 DEG C -100 DEG C of water bath with thermostatic control Heat 10min-15min；Mixture is put into supersonic extractors after heating water bath and carries out ultrasonic extraction, the ultrasonic extraction Ultrasonic power density is 400w/L-700w/L；It is 130 DEG C -150 DEG C to be ultrasonically treated temperature；Be ultrasonically treated according to " open ultrasound into Row supersound process 15s- closing ultrasound 5s- opening ultrasounds carry out the processing mode that supersound process 15s " is 1 cycle and are repeated Processing carries out 20-30 circular treatment in total；

(3) mixture after being ultrasonically treated step (2) takes out, and is filtered after being cooled to room temperature, into filtrate according to quality Than for 1-3:The HCl solution that 1 addition mass concentration is 2%-5% is placed in water bath with thermostatic control under 50 DEG C -60 DEG C of temperature condition 30min-40min, it is 1 to be cooled to after room temperature according to volume ratio:1 is added the condition that petroleum ether-ether is 50 DEG C -70 DEG C in temperature Under carry out extraction 60min-70min, obtain extract I after taking ether layer recycling design；Into filter residue according to solid-liquid mass ratio be 1: 8-10 be added percentage by volume be 10% (v/v) -20% (v/v) petroleum ether, be placed in temperature be 65 DEG C -75 DEG C under conditions of Constant temperature extracts 70min-80min, and extract II is obtained after taking ether layer recycling design；Extract I and extract II are uniformly mixed After obtain the aliphatic acid.

4. the feed according to claim 3 for improving hunchbacked milk yield, which is characterized in that the pre- place of step (1) peanut Reason method is：By after peanut decladding with mass concentration be 1%-3% NaHCO₃Solution is 2-4 according to solid-liquid mass ratio:1-2 is mixed Close, grinding after drying to moisture content be 3%-5% to obtain the final product.

5. the feed according to claim 3 for improving hunchbacked milk yield, which is characterized in that step (1) the oil tea slag it is pre- Processing method is：According to solid-liquid mass ratio it is 2-4 by NaCl solution that oil tea slag and mass concentration are 1%-3%:1 is mixed Close, under conditions of 60 DEG C -70 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

6. the feed according to claim 3 for improving hunchbacked milk yield, which is characterized in that step (1) stool in mice Preprocess method is：The stool in mice that mouse moisture content is 2%-4% is pulverized, is then 5.5 × 10 with living bacteria count⁹~ 8.1×10⁹CFU/ml bacillus subtilises bacterium solution is 20-30 according to mass ratio:1 is mixed, and is detested under conditions of 25-27 DEG C NaCl solution that tunning and mass concentration are 1%-3% according to mass ratio is 3-5 after fermentation by aerobe fermentation 15d-20d:1 Mixed, under conditions of 65 DEG C -75 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

7. the feed according to claim 3 for improving hunchbacked milk yield, which is characterized in that step (1) Dairy Wastewater is floating The preprocess method of slag is：It is 2.5 × 10 that Dairy Wastewater, which is filtered, and takes filter residue and living bacteria count⁹~6.1 × 10⁹CFU/ Ml lactic acid bacterial liquids are 15-20 according to mass ratio:1 is mixed, anaerobic fermentation 20d-25d under conditions of 23-25 DEG C, fermentation It is afterwards the NaHCO of 1%-3% by tunning and mass concentration₃Solution is 3-5 according to mass ratio:1-2 is mixed, 45 Under conditions of DEG C -55 DEG C water-bath 2h-3h filter to take filter residue drying to moisture content be 3%-5% to obtain the final product.

8. the feed according to claim 3 for improving hunchbacked milk yield, which is characterized in that the pre- place of step (1) milk Reason method is：It is 3.5 × 10 by milk and living bacteria count⁹~6.7 × 10⁹CFU/ml saccharomycete bacterium solution is 18- according to mass ratio 23:1 is mixed, and ferment 10d-15d under conditions of 26-28 DEG C, by tunning and mass concentration is 1%-3% after fermentation NaHCO₃Solution is 2-6 according to mass ratio:2-3 is mixed, and water-bath 1h-2h under conditions of 45 DEG C -55 DEG C to obtain the final product.

9. preparing the preparation method of feed according to claims 1-8, which is characterized in that described method includes following steps：

(1) napier grass prepares fermentation napier grass：Fresh napier grass is shredded, is then 25-30 according to napier grass and probiotics mass ratio:1 adds Add probiotics I ingredients, is 23 DEG C -27 DEG C in temperature, humidity is fermented under conditions of being 15%-25%, and fermentation time is 25d-35d, drying is that 10%-15% obtains the fermentation napier grass to moisture content under the conditions of 50 DEG C -55 DEG C；The probiotics I Ingredient by the living bacteria count after activating be 2.6 × 10⁹~9.6 × 10⁹CFU/g aspergillus oryzaes and living bacteria count be 4.2 × 10⁹~8.5 × 10⁹CFU/g saccharomycete is 2-4 according to mass ratio:1-3 is formed；

(2) eucalyptus leaves prepare fermentation eucalyptus leaves：According to mass ratio it is 25-30 by fresh eucalyptus leaves and spray:1 mixing shreds, so It is afterwards 30-35 according to eucalyptus leaves mixture and probiotics mass ratio:1 addition II ingredient of probiotics is 25 DEG C -30 DEG C in temperature, Humidity is fermented under conditions of being 20%-30%, and fermentation time 30d-40d is dried under the conditions of 60 DEG C -65 DEG C to aqueous Rate is that 8%-18% obtains the fermentation eucalyptus leaves；The ingredient of the probiotics II by the living bacteria count after activating be 1.9 × 10⁹~9.2 × 10⁹CFU/g aspergillus oryzaes and living bacteria count are 2.4 × 10⁹~8.5 × 10⁹CFU/g bacillus subtilises according to Mass ratio is 5-8:2-4 is formed；

(3) cassava prepares fermentation cassava：According to mass ratio it is 1 by cassava skin and cassava stem:3-5 is mixed, then according to wood Potato mixture is 20-25 with probiotics mass ratio:1 addition III ingredient of probiotics is 25 DEG C -30 DEG C in temperature, humidity 22%- It ferments under conditions of 32%, fermentation time 28d-38d, it is 12%- that moisture content is arrived in drying under the conditions of 55 DEG C -60 DEG C 17% obtains the fermentation cassava；The ingredient of the probiotics III is 2.3 × 10 by the living bacteria count after activating⁹~7.4 × 10⁹CFU/g lactic acid bacterias and living bacteria count are 2.1 × 10⁹~8.3 × 10⁹CFU/g saccharomycete is 2-4 according to mass ratio:1-3 groups At；

(4) each material composition is weighed by the weight ratio, by major ingredient：Ferment napier grass, fermentation eucalyptus leaves, fermentation cassava, sunflower shell Powder and maize cob meal crush after being thoroughly mixed, and are screened by -100 mesh screen of 30 mesh, finally add additive, dry It is that 3%-5% obtains the feed to do moisture content.