CN108324829A - A kind of Hemorrhagic shock mixture and preparation method thereof containing ginseng - Google Patents
A kind of Hemorrhagic shock mixture and preparation method thereof containing ginseng Download PDFInfo
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Abstract
The invention discloses a kind of Hemorrhagic shock mixture containing ginseng, prepares raw material and is grouped as by the group of following parts by weight：20 30 parts of ginseng, 10 20 parts of paris polyphylla, 10 20 parts of green peel, 5 15 parts of pyrola, 5 15 parts of black nightshade, 5 10 parts of Chinese trumpet creeper, 35 parts of wishful grass.The preparation method of the present invention also provides a kind of Hemorrhagic shock mixture containing ginseng.The Hemorrhagic shock mixture of the present invention has supplementing qi and nourishing yin green blood, function of warming yang and gore removing dredging collateral, the effect of replenishing qi to invigorate the spleen, can significantly improve cardiac function, restores blood pressure and heart rate to normal level, is had a better effect to haemorrhagic shock tool.In addition, the Hemorrhagic shock mixture of the present invention selects natural traditional Chinese medicine raw material to be made, medicinal material compatibility is reasonable, meet traditional Chinese medicine and modern medicine study theory, can achieve the purpose that treating both manifestation and root cause of disease, and preparation process of the present invention is simple, efficacy stability, application easy to spread have preferable application prospect.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of Hemorrhagic shock mixture and preparation method thereof containing ginseng.
Background technology
Shock is body in the case where losing blood seriously and losing the strong pathogenic factor effect such as liquid, infection, wound, and effective circulating blood volume is anxious
It reduces sharply less, tissue blood perfusion wretched insufficiency leads to various important vital organs, cell function dysbolism and structural damage
Systemic pathological process.Shock is a clinically relatively conventional acute disease, although pathogenic factor mostly with pathogenesis complexity,
Microcirculation disorder is the common base that many types of shock occurs.During suffering a shock occurrence and development, body generates a series of pathology
Physiological change mainly has contractile force to reduce, Microcirculation blood volume is reduced, blood distribution is abnormal, oxygen-supplying amount reduces, it is apparent to cause
Ischemia reperfusion injury, inflammatory mediator are spread unchecked, in this pathologic process they mutually convert, reciprocal causation, lead to body
Many-sided disorder occurs for energy dyspoiesis, tissue ischemia, anoxic, neuro-humoral mechanism activation, organism metabolism and function, finally
It may lead to systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction (MODS), or even dead.
Shock caused by massive blood loss is known as haemorrhagic shock.Be common in bleeding caused by wound, digestive ulcerative bleeding,
Bleeding etc. caused by rupture of esophageal varices, obstetrical and gynecological disease.Shock whether occur after losing blood depend not only on to lose blood
Amount, additionally depends on the speed lost blood, shock often quick, a large amount of (they being more than the 30%-35% of total blood volume) and lose blood and again
Occur in the case of less than being replenished in time.
The drug therapy of existing market still wants means based on Western medicine, although Chinese medicine preparation also has much, safety
Property, therapeutic effect be difficult to ensure again, can play the role of effective seldom.
Ginseng is araliaceae ginseng plant, and the panaxoside contained in ginseng can make the hemorrhagic Shock Dogs time-to-live bright
It is aobvious to extend, the damage of the sarolemma, nuclear membrane, mitochondria of haemorrhagic shock cardiac muscle cell can be prevented, have the protection shock animals heart, brain,
The effect of kidney and liver, but existing research only shows the Panaxadiol Saponin contained in ginseng and ginsenoside Re to losing
Courage and uprightness shock has certain therapeutic effect, and there is no the reports that related ginsenoside Rb1 treats haemorrhagic shock；Xu Hong equalitys
People has studied protective effect (Journal of Zhengzhou of the sanchinoside E1 to infectious shock rat vitals
2014 Vol.49 No.3 of University.May), it was demonstrated that Rb1 can protect infectious shock rats'liver, lung tissue, mechanism
The generation of TNF-α may be inhibited related with reduction TLR4 mRNA expressions, equally, the document is also without research panaxoside
Therapeutic effects of the Rb1 to haemorrhagic shock.
The present invention use new and effective extracting method, the sanchinoside E1 contained in ginseng is extracted, and and its
Its Chinese medicine material compatible use, animal experiments prove that, Hemorrhagic shock mixture of the invention has good control to haemorrhagic shock
Therapeutic effect.
Invention content
The present invention provides a kind of Hemorrhagic shock mixture containing ginseng, solves the drug therapy in market in the prior art still
Means are wanted based on Western medicine, although the problem of Chinese medicine preparation also has much, and safety, therapeutic effect are difficult to ensure.
The first purpose of the invention is to provide a kind of Hemorrhagic shock mixture containing ginseng, prepares raw material by following parts by weight
Several groups are grouped as：20-30 parts of ginseng, 10-20 parts of paris polyphylla, 10-20 parts of green peel, 5-15 parts of pyrola, black nightshade 5-15
Part, 5-10 parts of Chinese trumpet creeper, 3-5 parts of wishful grass.
Preferably, the raw material for preparing of the Hemorrhagic shock mixture containing ginseng is grouped as by the group of following parts by weight：Ginseng
25 parts, 15 parts of paris polyphylla, 15 parts of green peel, 10 parts of pyrola, 10 parts of black nightshade, 5 parts of Chinese trumpet creeper, 5 parts of wishful grass.
Second object of the present invention is to provide a kind of preparation method of the Hemorrhagic shock mixture containing ginseng, including following step
Suddenly：
Step 1,20-30 parts of ginseng, 10-20 parts of paris polyphylla, 10-20 parts of green peel, pyrola 5- are weighed by weight
15 parts, 5-15 parts of black nightshade, 5-10 parts of Chinese trumpet creeper, 3-5 parts of wishful grass；
Step 2, the ginseng weighed in step 1 is dry, smash it through 20 mesh sieve, obtain ginseng pulverate；By ginseng pulverate and water,
Lignin peroxidase is according to 100：50：1 mass ratio is uniformly mixed, and is subsequently placed at 60 DEG C and is digested 3h, and enzymolysis finishes
To enzymolysis product；Enzymolysis product is filtered, the first filtrate and filter residue are obtained, the second for being equivalent to 5 times of filter residue weight is added into filter residue
Alcoholic solvent ultrasonic extraction filters after extraction, obtains the second filtrate, merges the first filtrate and the second filtrate, is concentrated under reduced pressure into phase
It is 1.3-1.4 to density, obtains ginseng extract；
By ginseng extract and water according to 1g：Silicagel column is crossed after the ratio mixed dissolution of 10mL, is -0.5Mpa's in column pressure
Under the conditions of carry out silica gel column chromatography, be used in combination the mixed solution of ethyl alcohol and ethyl acetate to be eluted as eluant, eluent, collect fraction
And concentrate, dry, obtain ginsenoside crude product；
By ginsenoside crude product and n-butanol according to 1g：It is injected into after the ratio mixed dissolution of 5mL and efficiently prepares liquid phase color
It in spectrometer, is eluted using ethyl alcohol water mixed solution, collect fraction and concentrates, dries, obtain ginsenoside Rb1；
Step 3, the paris polyphylla weighed in step 1 is dry, smash it through 20 mesh sieve, obtain paris polyphylla powder,
It is added into paris polyphylla powder and is equivalent to the microorganism Aspergillus aculeatus culture solution that its weight is added 5%, ferment 5 under the conditions of 28-30 DEG C
It, fermentation finishes to obtain tunning；
Tunning and ethanol water are pressed into 1g：The ratio of 5mL mixes, and then flow back at 80 DEG C 3h, and reflux finishes
After filter, filtrate be concentrated into relative density be 1.3-1.4 to get Rhizoma Paridis extract；
Step 4, the green peel weighed in step 1, pyrola, black nightshade, Chinese trumpet creeper, wishful grass are mixed, obtains mixture, so
The ethyl alcohol for being equivalent to 5 times of mixture quality is added into mixture afterwards, is filtered after being heated to reflux 3h, obtains filtrate, by filtrate decompression
Concentration removes ethyl alcohol, obtains mixed extract；
Step 5, it will mix and extract in Rhizoma Paridis extract in ginsenoside Rb1, step 3 in step 2 and step 4
Object mixes, and is then charged with equal with ginsenoside Rb1, Rhizoma Paridis extract and mixed extract total weight
Aqueous sucrose solution is uniformly mixed to get to the Hemorrhagic shock mixture.
Preferably, ultrasonic extraction conditions are 50 DEG C, power 200w, ultrasonic extraction 30min in the step 2.
Preferably, the enzyme activity of the lignin peroxidase is 10000U/mL.
Preferably, the blade diameter length ratio of the silicagel column is 1：5.
Preferably, living bacteria count >=10 in the microorganism Aspergillus aculeatus culture solution10CFU/mL。
Preferably, the mass concentration of the aqueous sucrose solution is 80%.
The pharmacological action of each raw material Chinese medicine and drug effect in the present invention：
1, ginseng：Mild-natured, sweet in flavor, slight bitter, tepor.Returns spleen, lung, the heart channel of Hang-Shaoyin.With reinforce vital energy, veins takes off admittedly, tonifying spleen benefit
Lung, promote the production of body fluid to quench thirst, tranquilize the mind and promote the intelligence the effect of.《Sheng Nong's herbal classic》It is expressly recited that " the main tonifying five zang organs of ginseng, soothe the nerves determine soul, only
Palpitation with fear, remove pathogenic factor, the happy intelligence development of improving eyesight, long term usage are made light of one's life by commiting suicide macrobiosis ".Ginsenoside is one of the principle active component of ginseng, ginseng
Ginsenoside Rb1 in saponin(e has protective effect to lipopolysaccharide-induced Rat Mesenteric Microcirculation obstacle, to rat acute kidney
Tubular damage has protective effect, can promote rabbit cartilage cell proliferation, inhibit melanocyte nitric oxide synthase expression, to maincenter
Nervous system injury plays the role of reparation, has preferable protective effect to reperfusion brain damage.
2, paris polyphylla：It is bitter, cool.Mild toxicity.The thoughts of returning home, liver, lung, stomach, large intestine channel.With internal heat anticance, swelling and pain relieving, clear
The effect of heat arresting convulsion, antitussive and antiasthmatic.It is more that paris polyphylla rhizome contains 18 kinds of amino acid such as steroid saponin, alanine and creatinine etc.
Kind ingredient, can control the diseases such as carbuncle swells tuberculosis chronic cough, traumatic injury, snakebite and bugbite, scrofula, osteomyelitis, be the master of Yunnan Baiyao
Want one of ingredient；In recent years, and find in succession its chemical composition diosgenin have good antitumor, antibacterial, anti-inflammatory and
Treat the physiological activities such as gastric ulcer, leukaemia, angiocardiopathy, sedation and analgesia, relieving cough and asthma and suppression essence.
3, green peel：Property is bitter, pungent, warm.Return liver, courage, stomach.With soothing the liver relieving stagnant Qi, the stagnant effect of disperse accumulationization.It is swollen for chest side of body
Bitterly, hernia pain, stagnation of QI due to dyspepsia, the illnesss such as abdominal distention.Show the effect of green peel relaxation gastrointestinal smooth muscle most through experimental study
By force, mechanism is to block m receptor, excitement α receptors, directly inhibit intestinal smooth muscle.In addition green peel is longer to holding time for boosting, energy
Excitement breathing, has Hemorrhagic shock, cardioexcitatory effect.
4, pyrola：It is sweet, bitter, warm.Return lung, stomach, liver and kidney channel.With qi-restoratives, kidney-nourishing, dispelling wind and eliminating dampness, promoting blood circulation for regulating menstruation, benefit
The effect of strong bone of kidney, cough-relieving, hemostasis.Lumbago due to the kidney deficiency, arthralgia pain due to rheumatism, muscles and bones impotence, new chronic cough is cured mainly to cough, spit blood, bleeding from five sense organs or subcutaneous tissue, collapsing
The diseases such as leakage, traumatic hemorrhage.Wintergreen can increase myocardial contractive power, and arrhythmia can also be such that myocardial contractive power enhances, and blood vessel is aobvious
Expansion is write, blood pressure declines.The injection perfusion of wintergreen can also fight the vasoconstrictor effects of norepinephrine, be allowed to expand
.Pyrola decocting liquid energy obviously increases mice's myocardial nutritional blood flow and tissue (liver,kidney,spleen, brain) blood flow, and can be bright
It is aobvious to increase plasma cAMP content.In addition, pyrola is to staphylococcus aureus, hemolytic streptococcus, pneumococcus, meningitis ball
Bacterium, shigella flexneri, typhoid bacillus and Pseudomonas aeruginosa etc. have inhibiting effect.
5, black nightshade：Cold in nature, bitter, micro-sweet, it is slightly poisonous.Has effects that clearing heat and detoxicating, diuresis.For sore and toxic, skin
Skin eczema, difficult urination, chronic senile tracheitis, leukorrhea, prostatitis, dysentery.There is anti-inflammatory effect to animal, it is contained
Solasodine can reduce the activity of vasopermeability and hyaluronidase；To the anaphylaxis of animal, burn property, histamine shock
There are certain protective effects, moreover it is possible to increase the survival rate of mouse islets element shock, and the formation of antibody can be promoted.Black nightshade is to golden yellow
Staphylococcus, shigella dysenteriae, typhoid bacillus, proteus, Escherichia coli, Pseudomonas aeruginosa, hog cholera bacillus have certain suppression
Bacterium acts on.
6, Chinese trumpet creeper：Acrid flavour, acid, it is cold in nature.Return liver, pericardium channel.Have effects that promoting circulation of blood stasis eliminatings, cooling blood, expelling wind.For passing through
Bi wei lump in the abdomen, postpartum breast are swollen, rubella is rubescent, pruitus, acne.The Flavonoid substances contained in Chinese trumpet creeper can accelerate animal body
Blood flow velocity, increase capillary network crosspoint, expand thin vessels caliber, have improves microcirculation, promoting blood circulation and removing blood stasis well
Effect.
7, wishful grass：It is pungent numb, slightly sour, cold in nature.Has effects that clearing heat and detoxicating, scattered block up hemostasis.Main sore swollen toxin, acute mastitis,
Traumatic injury, open fracture, traumatic hemorrhage, snakebite.
Chinese medicine thinks that the cause of disease of haemorrhagic shock can be summarized as following two aspects：1, cloudy anemia consumption prolonged illness is Kidney-Yin loses
Consumption, or because hemorrhage bleeding, profuse sweating, vomiting, cross rush down, the reasons such as excess of sexual intercourse,indulgence in sexual activities due to cause the big wound of cloudy blood, internal organs lose in supporting；Or the diseases caused by external factors six external factors which cause diseases
Heresy, enter innerization heat, excessive noxious heat, consume impairment of yin liquid.Sun without the moon do not give birth to, the moon damage must and sun, cause yin deficiency sun damage.Yang-energy loses in temperature
It is warm and cause to faint card, severe patient's yang-energy just left unattached, outward dispersion of deficient YANG and cause the exhaustion of vital energy at the critical stage of an illness.2, yang-energy prolonged illness on the decline or sudden serious illness impairment of yang gas consumption and
Cause positive extreme exhaustion of QI or yin deficiency affecting yang, yang-energy void lose cannot be warm and cause card of fainting.If yang-energy is on the decline, sun is not attached cloudy and takes off, then causes de-
Card.Chinese medicine takes differentiation of symptoms and signs for classification of syndrome to treat, and faints and demonstrate,proves mainly based on QI invigorating Wen Yang, disperse blood stasis and dredge collateral, the exhaustion of vital energy at the critical stage of an illness is then based on recuperating depleted yang.
Dialectical cognition of the Chinese prescription of the present invention according to Chinese medicine to shock, it then follows the compatibility theory of Chinese medicine, for the side of being made
The preparation that easy use and curative effect are stablized, enhances the usage amount of medicinal material.The prescription is dissipated using ginseng, paris polyphylla as monarch drug in a prescription
Cold analgesic, warming channel for arresting bleeding, dispelling wind and eliminating dampness, numbness relieving and pain relieving；Using green peel, pyrola as ministerial drug, invigorating qi for strengthening superficies, cool blood, hemostasis, enriching yin
It enriches blood, beneficial to spirit and marrow；Using black nightshade as adjutant, sensible analgesic, invigorate blood circulation, nourishing generate fluid, tonifying middle-Jiao and Qi, nourishing blood and tranquilization；To insult
Clouds flower, wishful grass are to make medicine, coordinating the drug actions of a prescription pharmacological property, and all medicine cooperations complement each other, act synergistically, play supplementing qi and nourishing yin green blood, temperature altogether
Positive disperse blood stasis and dredge collateral, replenish qi to invigorate the spleen and other effects, there is very notable curative effect to shock.
Compared with prior art, the beneficial effects of the present invention are：
(1) Hemorrhagic shock mixture of the invention has supplementing qi and nourishing yin green blood, function of warming yang and gore removing dredging collateral, real the effect of replenishing qi to invigorate the spleen
Cardiac function can be significantly improved, restores blood pressure and heart rate to normal level, haemorrhagic shock tool is had a better effect.
(2) herb mixture of the present invention selects high-quality Chinese medicine to be made, and selected medicinal material compatibility is suitable, meets traditional Chinese medicine and modern times
Medical research is theoretical, can achieve the purpose that treating both manifestation and root cause of disease, and the preparation process of the present invention is simple and easy to do, preparation efficacy stability, easily
In popularization and application, there is preferable potential applicability in clinical practice.
Specific implementation mode
In order to enable those skilled in the art to more fully understand, technical scheme of the present invention is practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Microorganism Aspergillus aculeatus culture solution used is obtained using conventional method culture in embodiment, and institute in following each embodiments
Experimental method is stated, is conventional method unless otherwise specified.
Embodiment 1
A kind of Hemorrhagic shock mixture containing ginseng, prepares raw material and is grouped as by the group of following parts by weight：25 parts of ginseng, seven
15 parts of leaf a flower, 15 parts of green peel, 10 parts of pyrola, 10 parts of black nightshade, 5 parts of Chinese trumpet creeper, 5 parts of wishful grass.
Specifically prepare in accordance with the following steps：
Step 1,25 parts of ginseng, 15 parts of paris polyphylla, 15 parts of green peel, 10 parts of pyrola, black nightshade 10 are weighed by weight
Part, 5 parts of Chinese trumpet creeper, 5 parts of wishful grass；
Step 2, the ginseng weighed in step 1 is dry, smash it through 20 mesh sieve, obtain ginseng pulverate；By ginseng pulverate and water,
Lignin peroxidase is according to 100：50：1 mass ratio is uniformly mixed, and is subsequently placed at 60 DEG C and is digested 3h, and enzymolysis finishes
To enzymolysis product；Enzymolysis product is filtered, the first filtrate and filter residue are obtained, the second for being equivalent to 5 times of filter residue weight is added into filter residue
Alcoholic solvent, ultrasonic extraction 30min under conditions of being then 200W in 50 DEG C, ultrasonic power, filters after extraction, obtains the second filter
Liquid merges the first filtrate and the second filtrate, and it is 1.3-1.4 to be concentrated under reduced pressure into relative density, obtains ginseng extract；
By ginseng extract and water according to 1g：It is 1 that blade diameter length ratio is crossed after the ratio mixed dissolution of 10mL：5 silicagel column, in column
Pressure carries out silica gel column chromatography under conditions of being -0.5Mpa, and the mixed solution of ethyl alcohol and ethyl acetate is used in combination to be carried out as eluant, eluent
Elution collects fraction and concentrates, dries, obtains ginsenoside crude product；
By ginsenoside crude product and n-butanol according to 1g：It is injected into after the ratio mixed dissolution of 5mL and efficiently prepares liquid phase color
It in spectrometer, is eluted using ethyl alcohol water mixed solution, collect fraction and concentrates, dries, obtain ginsenoside Rb1；
Step 3, the paris polyphylla weighed in step 1 is dry, smash it through 20 mesh sieve, obtain paris polyphylla powder,
The microorganism Aspergillus aculeatus culture solution for being equivalent to its weight 5% is added into paris polyphylla powder, ferments 5 days under the conditions of 28-30 DEG C, sends out
Ferment finishes to obtain tunning；
Tunning and ethanol water are pressed into 1g：The ratio of 5mL mixes, and then flow back at 80 DEG C 3h, and reflux finishes
After filter, filtrate be concentrated into relative density be 1.3-1.4 to get Rhizoma Paridis extract；
Step 4, the green peel weighed in step 1, pyrola, black nightshade, Chinese trumpet creeper, wishful grass are mixed, obtains mixture, so
The ethyl alcohol for being equivalent to 5 times of mixture quality is added into mixture afterwards, is filtered after being heated to reflux 3h, obtains filtrate, by filtrate decompression
Concentration removes ethyl alcohol, obtains mixed extract；
Step 5, it will mix and extract in Rhizoma Paridis extract in ginsenoside Rb1, step 3 in step 2 and step 4
Object mixes, and is then charged with equal with ginsenoside Rb1, Rhizoma Paridis extract and mixed extract total weight
, the aqueous sucrose solution that mass concentration is 80%, be uniformly mixed to get to the Hemorrhagic shock mixture.
Embodiment 2
A kind of Hemorrhagic shock mixture containing ginseng, prepares raw material and is grouped as by the group of following parts by weight：20 parts of ginseng, seven
20 parts of leaf a flower, 10 parts of green peel, 15 parts of pyrola, 5 parts of black nightshade, 8 parts of Chinese trumpet creeper, 4 parts of wishful grass.
The preparation method is the same as that of Example 1, the difference is that changing the formula in embodiment 1 into embodiment 2.
Embodiment 3
A kind of Hemorrhagic shock mixture containing ginseng, prepares raw material and is grouped as by the group of following parts by weight：30 parts of ginseng, seven
10 parts of leaf a flower, 20 parts of green peel, 5 parts of pyrola, 15 parts of black nightshade, 10 parts of Chinese trumpet creeper, 3 parts of wishful grass.
The preparation method is the same as that of Example 1, the difference is that changing the formula in embodiment 1 into embodiment 3.
It needs, the enzyme activity of lignin peroxidase is 10000U/mL, is effectively lived in microorganism Aspergillus aculeatus culture solution
Bacterium number >=1010CFU/mL。
Effect in order to further illustrate the present invention is also provided with comparative example in experiment, specific as follows.
Comparative example 1
It is formulated and the preparation method is the same as that of Example 1, the difference is that being free of ginseng in formula.
Comparative example 2
Formula is with embodiment 1, and in the step 2 of difference preparation method, the preparation method of ginseng extract is as follows：
By the ginseng weighed in step 1 drying, 20 mesh sieve is smashed it through, obtains ginseng pulverate；By ginseng pulverate and water, lignin
Peroxidase is according to 100：50：1 mass ratio is uniformly mixed, and is subsequently placed at 60 DEG C and is digested 3h, and enzymolysis, which finishes, to be digested
Product；Enzymolysis product is filtered, the first filtrate and filter residue are obtained, the alcohol solvent for being equivalent to 5 times of filter residue weight is added into filter residue
Ultrasonic extraction filters after extraction, obtains the second filtrate, merges the first filtrate and the second filtrate, is concentrated under reduced pressure into relative density
For 1.3-1.4, ginseng extract is obtained；
Step 5, it will mix and extract in Rhizoma Paridis extract in ginseng extract, step 3 in step 2 and step 4
Object mixes, and is then charged with equal with ginseng extract, Rhizoma Paridis extract and mixed extract total weight
Aqueous sucrose solution is uniformly mixed to get to the Hemorrhagic shock mixture.
Comparative example 3
It is formulated and the preparation method is the same as that of Example 1, the difference is that being free of green peel, pyrola and black nightshade in formula.
Comparative example 4
It is formulated and the preparation method is the same as that of Example 1, the difference is that being free of Chinese trumpet creeper and wishful grass in formula.
Embodiment 1-3 has prepared the Hemorrhagic shock mixture containing ginseng to work well, uses zoopery to reality below
The performance for applying the Hemorrhagic shock mixture containing ginseng that a 1-3 is prepared is tested, and is illustrated with the effect to the present invention.
1, experimental method
100 SPF grades of SD rats are chosen, weight is 280 ± 10g, and half male and half female is randomly divided into Normal group, model
Group, positive controls, embodiment 1-3 groups, comparative example 1-4 groups, totally 10 groups, every group 10.In addition to Normal group, remaining each group
Rat weigh respectively, anaesthetized with 1% yellow Jackets (35mg/kg), dorsal position is fixed on 37 DEG C of constant temperature blankets, electrocardiogram electricity
Pole is connected on both sides lower limb and right upper extremity to record electrocardiographic wave respectively.Row tracheotomy after preserved skin, disinfection, trachea cannula are protected
Stay autonomous respiration.Right neck and double lower limb nervus vasculairs are detached respectively.MAP is connected after right carotid merging 22G ductus venosuses
Analysis of Cardiac Functions instrument monitors mean arterial pressure.Left femoral vein gives vasoactive agent as recovery channel and feeds back liquid, blood
Liquid.1KU/kg heparin sodiums make whole blood test tube of hepari through left femoral vein injection.Right femoral artery connects precision after being placed in 24G ductus venosuses
Infusion apparatus, bloodletting to shock.
The rat difference gavage of embodiment 1-3 groups is equal to Hemorrhagic shock mixture, dosage made from 1-3 of the embodiment of the present invention
For 20mg/kg, it is administered immediately after shock；The rat difference gavage of comparative example 1-4 groups is closed to Hemorrhagic shock made from comparative example 1-4
Agent, dosage are 20mg/kg, are administered immediately after shock；To adrenaline, add to pharmaceutical quantities is positive controls gavage
20mg/kg, Normal group and model group give the physiological saline of same volume, the daily gavage of each group 1 time, continuous 5 days.
In order to determine therapeutic effect of the Hemorrhagic shock mixture of the present invention to haemorrhagic shock, respectively before rat shock, shock
When and shock after detect rat heart rate and mean arterial pressure.
Statistical procedures test data is all made of mean ± standard deviation to indicate, group difference conspicuousness is examined with t to be compared,
Data processing is carried out using SPSS19.0 statistical softwares.
2, test result
Test result is as shown in table 1-2.
Influence of the table 1 to rat heart rate (HR)
Note：Compared with Normal group, * P<0.05；Compared with model group, * * P<0.01.
As can be seen from Table 1, the decline of Hemorrhagic Shock rat HR when suffering a shock 30min is the most notable, stops completely
It is all dead after gram；And gavage can dramatically increase hemorrhagic shock rats HR to the Hemorrhagic shock mixture of 1-3 of the embodiment of the present invention, make
It is restored to normal level, and therapeutic effect is suitable with commercially available adrenergic therapeutic effect.
Rat HR is significantly reduced after 1 Hemorrhagic shock mixture of gavage comparative example, and is difficult to be restored to normal level, illustrates that ginseng exists
Effect played in entire Hemorrhagic shock mixture is very important, and lacks ginseng, although Hemorrhagic shock mixture therapeutic effect is better than mould
Type group, but more other groups of effects are far short of what is expected.
Rat HR is reduced more after 2 Hemorrhagic shock mixture of gavage comparative example, although good compared with comparative example 1 and model group, than it
It is poor that it is organized, and illustrates that the ginsenoside Rb1 contained in ginseng is to treat the main component of haemorrhagic shock, also, traditional Chinese medicinal components are said
Study carefully compatibility, pure ginsenoside Rb1 is for the ginseng extract not purified, the effect with other raw material components
More preferably.
Rat HR is reduced less after 4 Hemorrhagic shock mixture of gavage comparative example 3 and comparative example, and effect is better than comparative example 1 and comparison
Example 2, but it is poorer than embodiment 1-3, illustrate that green peel, pyrola, black nightshade, Chinese trumpet creeper and wishful grass play in whole system respectively
Self-applying, and under the compatibility of the present invention, each bulk pharmaceutical chemicals interaction makes the effect for the Hemorrhagic shock mixture prepared reach most
It is good.
Influence of the table 2 to rat mean arterial pressure (MAP)
| Group | Dosage (mg/kg) | Before shock (mmHg) | Suffer a shock 30min (mmHg) | After shock (mmHg) |
| Embodiment 1 | 20 | 113±21 | 78±10** | 92±12* |
| Embodiment 2 | 20 | 115±18 | 76±12** | 98±11* |
| Embodiment 3 | 20 | 112±19 | 82±8** | 95±11* |
| Comparative example 1 | 20 | 111±22 | 58±12** | 65±9* |
| Comparative example 2 | 20 | 113±20 | 64±10** | 76±8* |
| Comparative example 3 | 20 | 113±19 | 71±11** | 89±11* |
| Comparative example 4 | 20 | 114±18 | 74±12** | 90±13* |
| Normal group | / | 112±21 | 112±17 | 112±17 |
| Model group | / | 116±28 | 42±8* | It is all dead |
| Positive controls | 20 | 115±23 | 69±10** | 93±12* |
Note：Compared with blank control group, * P<0.05；Compared with model group, * * P<0.01.
As can be seen from Table 2, the decline of Hemorrhagic Shock rat MAP when suffering a shock 30min is the most notable, stops completely
It is all dead after gram；And gavage can dramatically increase hemorrhagic shock rats MAP to the Hemorrhagic shock mixture of 1-3 of the embodiment of the present invention,
It is set to be restored to normal level, therapeutic effect is suitable with commercially available adrenergic therapeutic effect.
Rat MAP is significantly reduced after 1 Hemorrhagic shock mixture of gavage comparative example, and is difficult to be restored to normal level, illustrates ginseng
Effect played in entire Hemorrhagic shock mixture is very important, and lacks ginseng, although Hemorrhagic shock mixture therapeutic effect is better than
Model group, but more other groups of effects are far short of what is expected.
Rat MAP is reduced more after 2 Hemorrhagic shock mixture of gavage comparative example, although good compared with comparative example 1 and model group, than it
It is poor that it is organized, and illustrates that the ginsenoside Rb1 contained in ginseng is to treat the main component of haemorrhagic shock, also, traditional Chinese medicinal components are said
Study carefully compatibility, pure ginsenoside Rb1 is for the ginseng extract not purified, the effect with other raw material components
More preferably.
Rat MAP is reduced less after 4 Hemorrhagic shock mixture of gavage comparative example 3 and comparative example, and effect is better than comparative example 1 and comparison
Example 2, but it is poorer than embodiment 1-3, illustrate that green peel, pyrola, black nightshade, Chinese trumpet creeper and wishful grass play in whole system respectively
Self-applying, and under the compatibility of the present invention, each bulk pharmaceutical chemicals interaction makes the effect for the Hemorrhagic shock mixture prepared reach most
It is good.
It should be noted that involved in claims of the present invention when numberical range, it is thus understood that each numberical range
Any one numerical value can be selected between two endpoints and two endpoints, due to step method and the embodiment 1-3 phases of use
Together, it repeats in order to prevent, the present invention describes preferred embodiment, and once a person skilled in the art knows basic wounds
The property made concept, then additional changes and modifications may be made to these embodiments.So the following claims are intended to be interpreted as includes
Preferred embodiment and all change and modification for falling into the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of Hemorrhagic shock mixture containing ginseng, which is characterized in that it prepares raw material and is grouped as by the group of following parts by weight：People
Ginseng 20-30 parts, 10-20 parts of paris polyphylla, 10-20 parts of green peel, 5-15 parts of pyrola, 5-15 parts of black nightshade, 5-10 parts of Chinese trumpet creeper,
3-5 parts of wishful grass.
2. the Hemorrhagic shock mixture according to claim 1 containing ginseng, which is characterized in that it prepares raw material by following parts by weight
Several groups are grouped as：25 parts of ginseng, 15 parts of paris polyphylla, 15 parts of green peel, 10 parts of pyrola, 10 parts of black nightshade, 5 parts of Chinese trumpet creeper,
5 parts of wishful grass.
3. the preparation method of the Hemorrhagic shock mixture according to claim 1 containing ginseng, which is characterized in that specifically according to following
Step is implemented：
Step 1, weigh by weight 20-30 parts of ginseng, 10-20 parts of paris polyphylla, 10-20 parts of green peel, 5-15 parts of pyrola,
5-15 parts of black nightshade, 5-10 parts of Chinese trumpet creeper, 3-5 parts of wishful grass；
Step 2, the ginseng weighed in step 1 is dry, smash it through 20 mesh sieve, obtain ginseng pulverate；By ginseng pulverate and water, wooden
Plain peroxidase is according to 100：50：1 mass ratio is uniformly mixed, and is subsequently placed at 60 DEG C and is digested 3h, and enzymolysis finishes to obtain enzyme
Solve product；Enzymolysis product is filtered, the first filtrate and filter residue are obtained, into filter residue be added be equivalent to 5 times of filter residue weight ethyl alcohol it is molten
Agent ultrasonic extraction filters after extraction, obtains the second filtrate, merges the first filtrate and the second filtrate, is concentrated under reduced pressure into relatively close
Degree is 1.3-1.4, obtains ginseng extract；
By ginseng extract and water according to 1g：Silicagel column is crossed after the ratio mixed dissolution of 10mL, the condition for being -0.5Mpa in column pressure
Lower carry out silica gel column chromatography, is used in combination the mixed solution of ethyl alcohol and ethyl acetate to be eluted as eluant, eluent, and collection fraction is simultaneously dense
Contracting, drying, obtain ginsenoside crude product；
By ginsenoside crude product and n-butanol according to 1g：It is injected into high performance preparative liquid chromatography instrument after the ratio mixed dissolution of 5mL
In, it is eluted using ethyl alcohol water mixed solution, collects fraction and concentrate, dry, obtain ginsenoside Rb1；
Step 3, the paris polyphylla weighed in step 1 is dry, smash it through 20 mesh sieve, paris polyphylla powder is obtained, toward seven
The microorganism Aspergillus aculeatus culture solution for being equivalent to its weight 5% is added in leaf a flower powder, ferments 5 days, has fermented under the conditions of 28-30 DEG C
Finish and obtains tunning；
Tunning and ethanol water are pressed into 1g：The ratio of 5mL mixes, and then flow back at 80 DEG C 3h, mistake after reflux
Filter, filtrate be concentrated into relative density be 1.3-1.4 to get Rhizoma Paridis extract；
Step 4, the green peel weighed in step 1, pyrola, black nightshade, Chinese trumpet creeper, wishful grass are mixed, obtains mixture, it is then past
The ethyl alcohol for being equivalent to 5 times of mixture quality is added in mixture, is filtered after being heated to reflux 3h, obtains filtrate, filtrate decompression is concentrated
Ethyl alcohol is removed, mixed extract is obtained；
Step 5, mixed extract in Rhizoma Paridis extract in ginsenoside Rb1, step 3 in step 2 and step 4 is mixed
It closes, is then charged with the sucrose equal with ginsenoside Rb1, Rhizoma Paridis extract and mixed extract total weight
Aqueous solution is uniformly mixed to get to the Hemorrhagic shock mixture.
4. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that in the step 2
Ultrasonic extraction conditions are 50 DEG C, power 200w, ultrasonic extraction 30min.
5. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the lignin mistake
The enzyme activity of oxide enzyme is 10000U/mL.
6. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the silicagel column
Blade diameter length ratio is 1：5.
7. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the microorganism Aspergillus aculeatus
Living bacteria count >=10 in culture solution10CFU/mL。
8. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the sucrose is water-soluble
The mass concentration of liquid is 80%.
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Application publication date: 20180727 |