CN108293873A - A kind of selection of black sweet-waxy maizes - Google Patents
A kind of selection of black sweet-waxy maizes Download PDFInfo
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- CN108293873A CN108293873A CN201810424021.4A CN201810424021A CN108293873A CN 108293873 A CN108293873 A CN 108293873A CN 201810424021 A CN201810424021 A CN 201810424021A CN 108293873 A CN108293873 A CN 108293873A
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- black
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- waxy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of selections of black sweet-waxy maizes, belong to agriculture new variety selective breeding technology field, are related to corn selection and breeding.A kind of selection of black sweet-waxy maizes, step are：（1）It selects corn core self-mating system to be hybridized with Waxy Corn Inbred Lines, obtains hybridization grain；（2）Hybridization grain is continued into the sowing next generation, kind is all selfed at head progeny row, and purple, black glutinous seed is filtered out with Micro trauma simple grain iodine solution identification method；（3）It is selfed after purple, black glutinous seed is continued monoseeding, takes black shrinkage sweet tea grain, the as glutinous double recessive seed of sweet tea；（4）The glutinous double recessive seed of sweet tea is subjected to monoseeding, inbred line breeding obtains the glutinous double recessive self-mating system of sweet tea；（5）Be female parent with yellow, white Waxy Corn Inbred Lines, using the glutinous double recessive self-mating system of sweet tea as male parent, cultivate to obtain the final product.The method of the present invention is easy to operate, selection and breeding are quick, high yield, the new varieties of institute's selection and breeding rich in anthocyanidin, sugariness is high, glutinous grain is viscous, good quality, can meet the needs of production and market.
Description
Technical field
The invention belongs to agriculture new variety selective breeding technology fields, are related to corn selection and breeding, and in particular to a kind of glutinous jade of black sweet tea
The selection of rice.
Technical background
Anthocyanidin is a kind of water colo(u)r, belongs to the flavonoid class in phenolic compound, is widely present in plant kingdom,
Its is bright-colored, non-toxic, takes on a red color in acid condition or purple, is in blue under alkaline condition.In its natural state, flower
Green element is combined to form anthocyanin in plant with various monosaccharide.The anthocyanidin of anthocyanin class pigment is a kind of strong antioxygen
Agent can protect human body from the noxious insult of free radical, can enhance blood vessel elasticity, improve the circulatory system, inhibit inflammation
And allergy, enhance the flexibility in joint, has the function of anti-mutation, mitigates hepatosis and ascorbic acid.Therefore, anthocyanidin
With unique nutritive value and medical value, to maintaining normal human body physiological function to play an important roll.
With the development of sweet-waxy maizes industry, the requirement to sweet-waxy maizes kind is higher and higher, and product demand is more and more prosperous,
Cultivated area increases year by year, and process scale is increasing, and market prospects are very good.Rich in anthocyanidin sweet-waxy maizes because seed contains
There is higher anthocyanidin, and make its seed in black, purple, blue, red, therefore people call the corn rich in anthocyanidin
Blank corn, also referred to as Qarnet rice.Black sweet-waxy maizes have that color and luster is unique, savory, sweet tea is viscous, fresh and tender, palatability is good, full of nutrition etc.
Feature is natural black healthy food, high due to being rich in anthocyanidin sweet-waxy maizes nutritive value, therefore its utilization has bigger
Market, higher benefit, more tempting development prospect.
Currently, the selection and breeding of black sweet-waxy maizes are mainly hybridized using white, the glutinous double recessive self-mating systems of Huang Tian with purple, black waxy corn
Selection and breeding new varieties are carried out, but due to being restricted by purple, the limited germ plasm resource of black waxy corn so that this glutinous rich in anthocyanidin sweet tea
The variety development relative difficulty of corn.
In order to solve existing production and single white sweet tea is glutinous in the market, color sweet-waxy maizes type, and rich in anthocyanidin
The problem of waxy maize germplasm is deficient, and breeding of new variety speed cannot meet the tight market demand, having developed one kind can be quick
It formulates a large amount of cross combinations and therefrom selects high yield, the good selection rich in the black sweet-waxy maizes new varieties of anthocyanidin.
Invention content
The object of the present invention is to provide a kind of selections of black sweet-waxy maizes.
The present invention is achieved by the following technical solutions：
A kind of selection of black sweet-waxy maizes, step are：
（1）It selects corn core self-mating system to be hybridized with Waxy Corn Inbred Lines, obtains hybridization grain；
（2）Hybridization grain is continued into the sowing next generation, kind is all selfed at head progeny row, is filtered out with Micro trauma simple grain iodine solution identification method
Purple, black glutinous seed；
（3）It is selfed after purple, the black glutinous seed filtered out is continued monoseeding, black shrinkage sweet tea grain is chosen from selfing fruit ear,
The as glutinous double recessive seed of sweet tea；
（4）The glutinous double recessive seed of sweet tea is subjected to monoseeding, conventional inbred line breeding is carried out, obtains the glutinous double recessive self-mating system of sweet tea；
（5）It is female parent with yellow, white Waxy Corn Inbred Lines, using the glutinous double recessive self-mating system of sweet tea as male parent, cultivates up to anthocyanidin is rich in
Black sweet-waxy maizes.
Further, the corn is yellow or white corn core self-mating system, and the waxy corn is purple
Color or black Waxy Corn Inbred Lines.
The corn is yellow super-sweet corn self-mating system HT-01 or white super-sweet corn self-mating system BT-03.
The waxy corn is black waxy corn self-mating system HNF.
The step 4）The glutinous double recessive seed of sweet tea is subjected to monoseeding, 7 or 8 generations of selfing carry out selection and breeding.
The flows of selection and breeding of the present invention is as shown in Figure 1, first with yellow, white corn core self-mating system and purple, black glutinous
Corn inbred line is hybridized, and will continue the sowing next generation after hybridization fruit ear harvest, kind is all selfed at head progeny row, uses Micro trauma
Simple grain iodine solution identification method filters out purple, black glutinous seed；It is selfed after the seed filtered out is continued monoseeding, from selfing fruit ear
Upper selection black shrinkage sweet tea grain is the glutinous double recessive seed of sweet tea, enters conventional selfing after the sweet tea seed of selection is carried out monoseeding
It is in selection and breeding process；It is female parent with excellent yellow, white Waxy Corn Inbred Lines, is selfed rich in the glutinous double recessive of anthocyanidin sweet tea with institute's selection and breeding
System is that male parent carries out largely assembling screening, is cultivated at rich in anthocyanidin sweet-waxy maizes new varieties.I.e. the present invention utilizes Micro trauma list
Grain identification method filters out the glutinous seed of black from hybridization fruit ear, and monoseeding selfing selects sweet tea grain, as from selfing fruit ear
The glutinous double recessive material of sweet tea of primary election avoids and directly chooses the selfing of sweet tea grain from hybridization fruit ear, sweet tea, glutinous separation on offspring's fruit ear
And the problem of sweet tea seed emergence difficult；And breach and made male parent with white, the glutinous double recessive materials of Huang Tian in the past, by black, purple glutinous jade
The limitation of rice germ plasm resource scarcity and the problem of can not largely assemble the combination of black sweet-waxy maizes and screen new varieties.
The method of the present invention is easy to operate, selection and breeding are quick, high yield, and the new varieties of institute's selection and breeding are rich in anthocyanidin, sugariness height, glutinous grain
Viscous, good quality, can meet the needs of production and market.
Description of the drawings
Fig. 1 is the selection and breeding flow chart of the present invention.
Specific implementation mode
The present invention is further detailed with reference to specific embodiment, it is to be understood that these embodiments are only used
In illustrating the present invention rather than limit the scope of the invention.Those skilled in the art on the basis of the present invention make the present invention
Various changes or modification, should all equally fall within protection scope of the present invention.
Embodiment 1
A kind of selection of black sweet-waxy maizes, step are：
（1）It selects yellow super-sweet corn self-mating system HT-01 to make male parent with black waxy corn self-mating system HNF to hybridize, obtains hybridization seed；
（2）Bagging is selfed after above-mentioned hybridization seed is planted, using thorn break in the seed coat iodine solution detection Micro trauma method from seed
The glutinous seed of black is picked out on the selfing fringe of shape separation；
（3）Bagging after the glutinous seed plantation of the above-mentioned black picked out is selfed, black shrinkage sweet tea seed is selected from selfing fruit ear,
The glutinous double recessive material of black sweet tea of as primary election, is named as BDH170；
（4）White with the abundant backbone of quantity, yellow waxy corn after DH170 elected continuously 7 generations of selfing is largely hybridized
It is measured, filters out the high-quality black sweet-waxy maizes new varieties rich in anthocyanidin, be named as the glutinous 631-2 of black sweet tea.
After measured, chlorination Cyanidin content is 452mg/kg, peonidin 120mg/kg in the glutinous 631-2 of black sweet tea.
Embodiment 2
A kind of selection of black sweet-waxy maizes, step are：
（1）It selects white super-sweet corn self-mating system BT-03 to make male parent with black waxy corn self-mating system HNF to hybridize, obtains hybridization seed；
（2）Bagging is selfed after above-mentioned hybridization seed is planted, using thorn break in the seed coat iodine solution detection Micro trauma method from seed
The glutinous seed of black is picked out on the selfing fringe of shape separation；
（3）Bagging after the glutinous seed plantation of the above-mentioned black picked out is selfed, black shrinkage sweet tea seed is selected from selfing fruit ear
The glutinous double recessive material BDH30 of black sweet tea of as primary election；
（4）White with the abundant backbone of quantity, yellow waxy corn after BDH30 elected continuously 8 generations of selfing is largely hybridized
It is measured, filters out the high-quality black sweet-waxy maizes new varieties rich in anthocyanidin --- black sweet tea glutinous 639.
After measured, chlorination Cyanidin content is 332mg/kg, peonidin 67mg/kg in black sweet tea glutinous 639.
Claims (5)
1. a kind of selection of black sweet-waxy maizes, which is characterized in that step is：
（1）It selects corn core self-mating system to be hybridized with Waxy Corn Inbred Lines, obtains hybridization grain；
（2）Hybridization grain is continued into the sowing next generation, kind is all selfed at head progeny row, is filtered out with Micro trauma simple grain iodine solution identification method
Purple, black glutinous seed；
（3）It is selfed after purple, the black glutinous seed filtered out is continued monoseeding, black shrinkage sweet tea grain is chosen from selfing fruit ear,
The as glutinous double recessive seed of sweet tea；
（4）The glutinous double recessive seed of sweet tea is subjected to monoseeding, conventional inbred line breeding is carried out, obtains the glutinous double recessive self-mating system of sweet tea；
（5）It is female parent with yellow, white Waxy Corn Inbred Lines, using the glutinous double recessive self-mating system of sweet tea as male parent, cultivates up to the glutinous jade of black sweet tea
Rice.
2. a kind of selection of black sweet-waxy maizes according to claim 1, which is characterized in that the corn is
Yellow or white corn core self-mating system, the waxy corn are purple or black Waxy Corn Inbred Lines.
3. a kind of selection of black sweet-waxy maizes according to claim 1 or 2, which is characterized in that the sweet tea is beautiful
Rice is yellow super-sweet corn self-mating system HT-01 or white super-sweet corn self-mating system BT-03.
4. a kind of selection of black sweet-waxy maizes according to claim 1 or 2, which is characterized in that the glutinous jade
Rice is black waxy corn self-mating system HNF.
5. a kind of selection of black sweet-waxy maizes according to claim 1 or 2, which is characterized in that the step
4）The glutinous double recessive seed of sweet tea is subjected to monoseeding, 7 or 8 generations of selfing carry out selection and breeding.
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| CN201810424021.4A CN108293873A (en) | 2018-05-07 | 2018-05-07 | A kind of selection of black sweet-waxy maizes |
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| CN201810424021.4A CN108293873A (en) | 2018-05-07 | 2018-05-07 | A kind of selection of black sweet-waxy maizes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110132963A (en) * | 2019-04-26 | 2019-08-16 | 安徽省农业科学院烟草研究所 | A kind of method of Rapid identification waxy corn germplasm |
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| CN1930950A (en) * | 2006-07-20 | 2007-03-21 | 王志刚 | Breeding process of fruit-vegetable type sweet glutinous hybrid corn |
| CN104521742A (en) * | 2014-12-26 | 2015-04-22 | 广东省农业科学院作物研究所 | Method for rapidly creating sweet-waxy recessive maize inbred line |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| US20160135402A1 (en) * | 2014-11-13 | 2016-05-19 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid sv1446sd and parents thereof |
| CN106359076A (en) * | 2016-08-30 | 2017-02-01 | 赫忠友 | Cultivating method for glutinous sweet and sweet corns |
| CN107360963A (en) * | 2016-05-13 | 2017-11-21 | 上海粒粒丰农业科技有限公司 | A kind of colored sweet glutinous maize self-bred line selection |
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2018
- 2018-05-07 CN CN201810424021.4A patent/CN108293873A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930950A (en) * | 2006-07-20 | 2007-03-21 | 王志刚 | Breeding process of fruit-vegetable type sweet glutinous hybrid corn |
| US20160135402A1 (en) * | 2014-11-13 | 2016-05-19 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid sv1446sd and parents thereof |
| CN104521742A (en) * | 2014-12-26 | 2015-04-22 | 广东省农业科学院作物研究所 | Method for rapidly creating sweet-waxy recessive maize inbred line |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN107360963A (en) * | 2016-05-13 | 2017-11-21 | 上海粒粒丰农业科技有限公司 | A kind of colored sweet glutinous maize self-bred line selection |
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Non-Patent Citations (2)
| Title |
|---|
| LERTRAT.K 等: "Novel Approach to Eating Quality Improvement in Local Waxy Corn:Improvement of Sweet Taste in Local Waxy Corn Variety with Mixed Kernels from Super Sweet Corn", 《ACTA HORTICULTURAE》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110132963A (en) * | 2019-04-26 | 2019-08-16 | 安徽省农业科学院烟草研究所 | A kind of method of Rapid identification waxy corn germplasm |
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Application publication date: 20180720 |