CN109729970A - A kind of selection of the glutinous double hidden corn inbred lines of higher survival rate sweet tea - Google Patents
A kind of selection of the glutinous double hidden corn inbred lines of higher survival rate sweet tea Download PDFInfo
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Abstract
The present invention provides a kind of selections of the glutinous double hidden corn inbred lines of higher survival rate sweet tea, are related to corn breeding techniques field.The method of the invention, comprising the following steps: the seed of single cross F1 that Waxy Corn Inbred Lines and big embryo hybridization between selfed lines obtain is done into female parent, then is hybridized with inbred line of sweet corn with the seed of single cross F1 that big embryo hybridization between selfed lines obtains, seed of double cross F1 is obtained；Big semina in seed of double cross F1 is screened, F2 generation is selfed to obtain；The seed of the F2 Dai Zhong great embryo shrinkage phenotype is screened, selects the plant of pollen iodine dye rate of dyeing 50% to be selfed, obtains F3 generation；The plant of pollen iodine dye rate of dyeing 100% is selfed in selection F3 generation, obtains F4 generation；Head progeny row of the emergence rate higher than 80% in F4 generation is selected, screening the big glutinous self-mating system of embryo sweet tea is F5 generation；The F5 was continuously selfed for 3~4 generations for single plant bagging, obtains the big glutinous self-mating system of embryo sweet tea.The glutinous double hidden corn inbred line emergence rates of sweet tea can be increased to 90% using the method for the invention.
Description
Technical field
The invention belongs to corn breeding techniques fields, and in particular to a kind of choosing of the glutinous double hidden corn inbred lines of higher survival rate sweet tea
Educate method.
Background technique
Sweet tea adds waxy corn to be nutrition and the flavor while China corn breeding man is bred as in recent years with Sweety and waxy corn
Novel fresh edible maize kind (has waxy corn seed and corn seed, the two ratio is 3:1) simultaneously on the same fruit ear, it is deep by
China consumer's likes, has had 3,000,000 mu of cultivated area in China, quickly forms waxy corn, corn, sweet tea and adds glutinous jade
The situation that rice is stood like the legs of a tripod, market potential are very big.
It is bred as sweet tea the primary condition of glutinous fresh-eating corn variety is added to be one of parents of cenospecies being the glutinous double hidden selfings of sweet tea
System, another parent is Waxy Corn Inbred Lines.However during breeding sweet tea glutinous double recessive self-mating system, no matter which kind of takes educate
Kind technology, the glutinous double recessive self-mating system (su1su1wxwx, sh2sh2wxwx, bt2bt2wxwx) of which kind of sweet tea of breeding are all difficult to solve
The bud ratio of one glutinous double recessive self-mating system of generally existing realistic problem-sweet tea is very low.The glutinous double recessive self-mating system of sweet tea is either done
Female parent still does male parent, and bud rate is low to directly affect hybrid seed yield, to increase breeding cost and sweet tea adds glutinous fresh-eating maize production
Cost.The low limitation for having become sweet tea and adding the most critical of glutinous novel fresh edible maize industry development of the glutinous double recessive self-mating system bud rate of sweet tea at present
Factor.
Breeding practice proves, when waxy corn gene (wxwx), corn gene (su1su1, sh2sh2) individualism, it
Bud ratio be well, when wxwx gene and when su1su1, sh2sh2 gene pyramiding homozygosis, su1su1wxwx,
The bud ratio meeting sharp fall of sh2sh2wxwx, finds the embryo ratio of the bis- hidden self-mating systems of su1su1wxwx, sh2sh2wxwx
Embryo when wxwx, su1su1, sh2sh2 individualism wants small.Press down when wxwx gene is with su1su1, sh2sh2 gene pure
The development of embryo is made, embryo is too small to cause the bis- hidden self-mating systems of su1su1wxwx, sh2sh2wxwx embryo in germination to provide battalion
Deficiency is supported, germination percentage is low.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of breeding sides of the glutinous double hidden corn inbred lines of higher survival rate sweet tea
Method significantly improves the germination percentage of the bis- hidden self-mating systems of su1su1wxwx, sh2sh2wxwx.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of selections of the glutinous double hidden corn inbred lines of higher survival rate sweet tea, comprising the following steps: (1)
Be female parent with the first seed of single cross F1 that Waxy Corn Inbred Lines and big embryo hybridization between selfed lines obtain, with inbred line of sweet corn and big embryo from
The second seed of single cross F1 for handing over system's hybridization to obtain is male parent, and the female parent is hybridized with the male parent, obtains seed of double cross F1；
(2) the big embryo character seed plantation in the seed of double cross F1 is screened, the selfing of single plant bagging obtains F2 generation；
(3) in the fruit ear in the F2 generation, the seed kind of big embryo shrinkage phenotype is screened into head progeny row, while to each plant
Powder carries out iodine staining, selects the plant of pollen staining rate 50% to carry out bagging selfing, obtains F3 generation；
(4) the F3 generation kind is carried out iodine staining to each single plant pollen, selects pollen staining rate 100% at head progeny row
Plant is selfed, and F4 generation is obtained；
(5) emergence rate is selected to be higher than 80% head progeny row at head progeny row the F4 generation plantation, obtaining the big glutinous self-mating system of embryo sweet tea is F5
Generation；
(6) F5 was continuously selfed for 3~4 generations for single plant bagging, obtains the glutinous double hidden corn inbred lines of higher survival rate sweet tea.
It preferably, include waxy corn gene wxwx in the Waxy Corn Inbred Lines.
It preferably, include corn gene su1su1 or sh2sh2 in the inbred line of sweet corn.
It preferably, include big blastema in the big embryo self-mating system because of gege.
The present invention provides a kind of selection of the glutinous double hidden corn inbred lines of higher survival rate sweet tea, hybridization and selfing are utilized
Big blastema is added because of gege, to significantly improve in Xiang Shuanyin self-mating system genotype su1su1wxwx and sh2sh2wxwx in means
The germination percentage of the glutinous double hidden self-mating systems of sweet tea.The big glutinous self-mating system of embryo sweet tea of corn variety can be obtained using selection of the present invention
ZBN6279 (genotype wxwxgegesh2sh2) and the glutinous self-mating system ZBT01 (genotype wxwxgegesu1su1) of big embryo sweet tea, experience
Card keeps the big embryo su1wx comprising double hidden self-mating system genotype su1su1wxwx and sh2sh2wxwx and big embryo sh2wx genotype beautiful
The germination percentage of rice is up to 90%.
Detailed description of the invention
Fig. 1 is the breeding process of the embodiment of the present invention 1；
Fig. 2 is the breeding process of the embodiment of the present invention 2.
Specific embodiment
The selection of the glutinous double hidden corn inbred lines of a kind of higher survival rate sweet tea, comprising the following steps: (1) be selfed with waxy corn
System is female parent with the first seed of single cross F1 that big embryo hybridization between selfed lines obtains, and is obtained with inbred line of sweet corn and big embryo hybridization between selfed lines
The second seed of single cross F1 be male parent, the female parent is hybridized with the male parent, acquisition seed of double cross F1；
(2) the big embryo character seed plantation in the seed of double cross F1 is screened, the selfing of single plant bagging obtains F2 generation；
(3) in the fruit ear in the F2 generation, the seed kind of big embryo shrinkage phenotype is screened into head progeny row, while to each plant
Powder carries out iodine staining, selects the plant of pollen staining rate 50% to carry out bagging selfing, obtains F3 generation；
(4) the F3 generation kind is carried out iodine staining to each single plant pollen, selects pollen staining rate 100% at head progeny row
Plant is selfed, and F4 generation is obtained；
(5) emergence rate is selected to be higher than 80% head progeny row at head progeny row the F4 generation plantation, obtaining the big glutinous self-mating system of embryo sweet tea is F5
Generation；
(6) F5 was continuously selfed for 3~4 generations for single plant bagging, obtains the glutinous double hidden corn inbred lines of higher survival rate sweet tea.
In selection of the present invention, with first seed of single cross of Waxy Corn Inbred Lines and big embryo hybridization between selfed lines acquisition
F1 is female parent, the second seed of single cross F1 obtained using inbred line of sweet corn and big embryo hybridization between selfed lines as male parent, by it is described it is maternal with
The male parent is hybridized, and seed of double cross F1 is obtained.It include waxy corn gene wxwx in Waxy Corn Inbred Lines of the present invention；It is described
It include corn gene su1su1 or sh2sh2 in inbred line of sweet corn；It include big blastema in the big embryo self-mating system because of gege.
Big embryo self-mating system of the present invention is high oil corn self interweaving series.The genotype of female parent of the present invention is preferably
WxwxGegeSu1Su1 or WxwxGegeSh2Sh2, the genotype of the male parent be preferably WxWxGegeSu1Su1 or
WxWxGegeSh2sh2.The present invention is hybridized using the male parent and female parent of the genotype, obtains seed of double cross F1, of the present invention
There is 3/4 to show as small embryo character in seed of double cross F1, genotype is Wx_Ge_Su1_ or Wx_Ge_Sh2_；In addition there is 1/4 quantity table
It is now big embryo character, genotype is Wx_gegeSu1_ or Wx_gegeSh2_, wherein " _ " indicates be that dominant gene can also be
Recessive gene.There is no particular determinations for method of the present invention to the hybridization, are using the ordinary maize hybridization means of this field
It can.
After obtaining seed of double cross F1, the present invention screens the plantation of the big embryo character seed in the seed of double cross F1, and single plant bagging is selfed,
Obtain F2 generation.It is also preferable to include shrinkages for the phenotype of big semina of the present invention.The present invention carries out big semina of the phenotype
Selfing, obtains F2 generation.There is no particular determinations for method of the present invention to the selfing, utilize the ordinary maize autocopulation method of this field
?.
After obtaining F2 generation, the present invention screens the seed kind of big embryo shrinkage phenotype at head progeny row, simultaneously in the fruit ear in the F2 generation
Iodine staining is carried out to each plant pollen, selects the plant of pollen staining rate 50% to carry out bagging selfing, obtains F3 generation.Institute of the present invention
State big embryo shrinkage phenotype seed cdna type be WxWxgegesu1su1, Wxwxgegesu1su1 or WxWxgegesh2sh2,
Wxwxgegesh2sh2.The present invention plants the F2 of the genotype for seed, and carries out iodine staining, selection to each plant pollen
The plant of pollen staining rate 50% carries out bagging selfing.Iodine of the present invention can dye completely wxwx, and half dyes Wxwx, cannot
The plant dyed WxWx, therefore screen rate of dyeing 50% carries out bagging selfing, and obtained F3 is for having 1/4 in seed cdna type
WxWxgegesu1su1 or WxWxgegesh2h2,1/2 is Wxwx gegesu1su1 or Wxwx gegesh2sh2, and 1/4 is
Wxwxgegesu1su1 or wxwxgegesh2sh2.At the method for head progeny row and iodine staining, there is no special limits to described kind by the present invention
It is fixed, utilize the conventional method of this field.
After obtaining F3 generation, the F3 generation kind at head progeny row, is carried out iodine staining, selection pollen dye to each single plant pollen by the present invention
The plant of color rate 100% is selfed, and F4 generation is obtained.The present invention select the plant i.e. screening-gene type of pollen staining rate 100% for
The plant of wxwx is selfed.
After obtaining F4 generation, the present invention by the F4 generation plantation at head progeny row, be selfed, obtain by the head progeny row that selection emergence rate is higher than 80%
The high double hidden systems of emergence rate.The F5 screened in the embodiment of the present invention for genotype be wxwxgegesu1su1 or
wxwxgegesh2sh2。
After obtaining F5 generation, the F5 was continuously selfed for 3~4 generations for single plant bagging by the present invention, obtained the glutinous double hidden jade of higher survival rate sweet tea
Rice self-mating system.Continuous selfing of the present invention can determine whether character can stablize heredity.
It is carried out below with reference to the selection of embodiment double hidden corn inbred lines glutinous to higher survival rate sweet tea provided by the invention
Detailed description, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Breeding is carried out according to process as shown in Figure 1:
1. hybridizing using waxy corn wxwxGeGeSh2Sh2 with big embryo corn WxWxgegeSh2Sh2, F1 generation corn is obtained
WxwxGegeSh2Sh2 is female parent with the F1 generation corn；
2. hybridizing using super-sweet corn WxWxGeGesh2sh2 with big embryo corn WxWxgegeSh2Sh2, F1 generation corn is obtained
WxWxGegeSh2sh2, using the F1 generation corn as male parent；
3. after above-mentioned female parent is hybridized with male parent, screen showed in F1 generation the corn Wx_Ge_Sh2_ of small embryo character into
Row selfing, selects big embryo shrinkage progeny corn WxWxgegesh2sh2 and Wxwxgegesh2sh2；
4. planting the big embryo shrinkage progeny corn, iodine staining is carried out to pollen, selects the Wxwx of pollen staining rate 50%
The selfing of genotype seed, obtains 1/4 WxWx gegesh2sh2,1/2 Wxwx gegesh2sh2 and 1/4
Wxwxgegesh2sh2 genotype offspring；
5. planting said gene type offspring, and iodine staining is carried out to pollen, selects the wxwx gene of pollen staining rate 100%
Type selfing, obtains wxwxgegesh2sh2 maize genotypes；
6. carrying out 3~4 generations of continuous selfing to above-mentioned wxwxgegesh2sh2 maize genotypes, the sweet tea that must stablize heredity is glutinous double
Hidden self-mating system wxwxgegesh2sh2 is named as the glutinous self-mating system ZBN6279 of big embryo sweet tea.
Embodiment 2
Breeding is carried out according to process shown in Fig. 2:
1. hybridizing using waxy corn wxwxGeGeSu1Su1 with big embryo corn WxWxgege Su1Su1, F1 generation corn is obtained
WxwxGegeSu1Su1 is female parent with the F1 generation corn；
2. hybridizing using super-sweet corn WxWxGeGeSu1Su1 with big embryo corn WxWxgegeSu1Su1, F1 generation corn is obtained
WxWxGegeSu1su1, using the F1 generation corn as male parent；
3. after above-mentioned female parent is hybridized with male parent, screen showed in F1 generation the corn Wx_Ge_Su1_ of small embryo character into
Row selfing, selects big embryo shrinkage progeny corn WxWxgegesu1su1 and Wxwxgegesu1su1；
4. planting the big embryo shrinkage progeny corn, iodine staining is carried out to pollen, selects the Wxwx of pollen staining rate 50%
The selfing of genotype seed, obtains 1/4 WxWx gegesu1su1,1/2 Wxwx gegesu1su1 and 1/4
Wxwxgegesu1su1 genotype offspring；
5. planting said gene type offspring, and iodine staining is carried out to pollen, selects the wxwx gene of pollen staining rate 100%
Type selfing, obtains wxwxgegesu1su1 maize genotypes；
6. carrying out 3~4 generations of continuous selfing to above-mentioned wxwxgegesu1su1 maize genotypes, the sweet tea that must stablize heredity is glutinous double
Hidden self-mating system wxwxgegesu1su1 is named as the glutinous self-mating system ZBT01 of big embryo sweet tea.
The big glutinous self-mating system of embryo sweet tea that embodiment 1 and embodiment 2 obtain is tested, its emergence rate, experimental result are verified
It is as shown in table 1:
Influence of 1 different genotype of table to emergence rate
| Self-mating system | su1 | sh2 | wx | Big embryo | su1wx | sh2wx | Big embryo su1wx | Big embryo sh2wx |
| Emergence rate % | 97 | 95 | 99 | 100 | 45 | < 40 | 90 | 90 |
The present invention provides a kind of selections of the glutinous double hidden corn inbred lines of higher survival rate sweet tea, can be by the glutinous double hidden corns of sweet tea
Self-mating system emergence rate is increased to 90%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (4)
1. a kind of selection of the glutinous double hidden corn inbred lines of higher survival rate sweet tea, which comprises the following steps: (1) with
The first seed of single cross F1 that Waxy Corn Inbred Lines and big embryo hybridization between selfed lines obtain is female parent, is selfed with inbred line of sweet corn and big embryo
The second seed of single cross F1 that system's hybridization obtains is male parent, and the female parent is hybridized with the male parent, obtains seed of double cross F1；
(2) the seed plantation of the big embryo character in the seed of double cross F1 is screened, the selfing of single plant bagging obtains F2 generation；
(3) in the fruit ear in the F2 generation, screen the seed kind of big embryo shrinkage phenotype into head progeny row, at the same to each plant pollen into
Row iodine staining selects the plant of pollen staining rate 50% to carry out bagging selfing, obtains F3 generation；
(4) the F3 generation kind is carried out iodine staining to each single plant pollen, selects the plant of pollen staining rate 100% at head progeny row
It is selfed, obtains F4 generation；
(5) emergence rate is selected to be higher than 80% head progeny row at head progeny row the F4 generation plantation, obtaining the big glutinous self-mating system of embryo sweet tea is F5 generation；
(6) F5 was continuously selfed for 3~4 generations for single plant bagging, obtains the glutinous double hidden corn inbred lines of higher survival rate sweet tea.
2. selection according to claim 1, which is characterized in that include waxy corn gene in the Waxy Corn Inbred Lines
wxwx。
3. selection according to claim 1, which is characterized in that include corn gene in the inbred line of sweet corn
Su1su1 or sh2sh2.
4. selection according to claim 1, which is characterized in that include big blastema in the big embryo self-mating system because of gege.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112655549A (en) * | 2021-01-08 | 2021-04-16 | 河南省农业科学院粮食作物研究所 | Method for breeding black, white and yellow homogeneous waxy corn inbred line |
| CN115226627A (en) * | 2022-09-26 | 2022-10-25 | 北京市农林科学院 | Method for efficiently creating sweet waxy corn inbred line |
| CN115316265A (en) * | 2022-08-19 | 2022-11-11 | 辽东学院 | Sweet corn crossbreeding method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655549A (en) * | 2021-01-08 | 2021-04-16 | 河南省农业科学院粮食作物研究所 | Method for breeding black, white and yellow homogeneous waxy corn inbred line |
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| CN115316265A (en) * | 2022-08-19 | 2022-11-11 | 辽东学院 | Sweet corn crossbreeding method |
| CN115226627A (en) * | 2022-09-26 | 2022-10-25 | 北京市农林科学院 | Method for efficiently creating sweet waxy corn inbred line |
| CN115226627B (en) * | 2022-09-26 | 2022-12-27 | 北京市农林科学院 | Method for creating sweet waxy corn inbred line and application |
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