CN116267589B - Cross breeding method of full-petal wide-tropical water lily - Google Patents
Cross breeding method of full-petal wide-tropical water lily Download PDFInfo
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- CN116267589B CN116267589B CN202310492162.0A CN202310492162A CN116267589B CN 116267589 B CN116267589 B CN 116267589B CN 202310492162 A CN202310492162 A CN 202310492162A CN 116267589 B CN116267589 B CN 116267589B
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- water lily
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- 241000209490 Nymphaea Species 0.000 title claims abstract description 53
- 235000016791 Nymphaea odorata subsp odorata Nutrition 0.000 title claims abstract description 48
- 238000009402 cross-breeding Methods 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 10
- 238000009395 breeding Methods 0.000 claims abstract description 15
- 238000009396 hybridization Methods 0.000 claims abstract description 14
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 12
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 12
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 12
- 230000010152 pollination Effects 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 8
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 5
- 241000087968 Nymphaea cyanea Species 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims 1
- 230000001488 breeding effect Effects 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 7
- 206010063659 Aversion Diseases 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000011835 investigation Methods 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 240000009085 Nymphaea caerulea Species 0.000 description 2
- 235000002195 Nymphaea caerulea Nutrition 0.000 description 2
- 241001183071 Nymphaea guineensis Species 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 244000295848 Citrus x nobilis Species 0.000 description 1
- 235000017102 Citrus x nobilis Nutrition 0.000 description 1
- 241000073851 Nuphar x spenneriana Species 0.000 description 1
- 241000740933 Nymphaea minuta Species 0.000 description 1
- 241000209477 Nymphaeaceae Species 0.000 description 1
- 240000007019 Oxalis corniculata Species 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000005811 Viola adunca Nutrition 0.000 description 1
- 240000009038 Viola odorata Species 0.000 description 1
- 235000013487 Viola odorata Nutrition 0.000 description 1
- 235000002254 Viola papilionacea Nutrition 0.000 description 1
- 244000172533 Viola sororia Species 0.000 description 1
- 101000737997 Zea mays Chalcone synthase WHP1 Proteins 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004578 fetal growth Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000036544 posture Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/121—Plant growth habits
- A01H1/1215—Flower development or morphology, e.g. flowering promoting factor [FPF]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a hybridization breeding method of full-petal wide-tropical water lily, which is to hybridize with Jiupin lotus as a female parent and blue star water lily as a male parent to obtain a new hybridization variety. The invention breeds a new full-petal wide-tropical water lily variety, which has obvious differences with the parent's variety in the aspects of flower color, flower diameter, re-petal property, leaf diameter and the like, has higher ornamental value, is very suitable for being used as cut flowers, can fill up the blank of China in the aspect of the new full-petal water lily variety, has good economic value and is worthy of popularization and application.
Description
Technical Field
The invention relates to the technical field of water lily cross breeding, in particular to a cross breeding method of full-petal wide-tropical water lily.
Background
The water lily (Nymphaea spp.) is a water lily genus (Nymphaea perennial raw water plant) of Nymphaea family (Nymphaeaceae), has large and beautiful flowers, gorgeous colors and beautiful postures, is a famous flower in aquatic flowers, is known as a sleeping beauty in flowers, is an indispensable important material in garden waterscape, has high cultural, ornamental and application values, and can be divided into 5 subgenera, namely cold-resistant subgenera, australian subgenera, wide tropical subgenera, ancient tropical subgenera and new tropical subgenera according to morphological characteristics, origin, habit and the like.
At present, the research and production of the water lily in China are very insufficient, and particularly the self-bred variety of the water lily in the tropical zone is deficient. The tropical water lily in China mainly comes from the United states, thailand and Australia, and along with the enhancement of the foreign awareness of germplasm resource protection, the difficulty of introducing good varieties from the foreign is increased, so that the acceleration of the breeding of the tropical water lily varieties in China is particularly urgent. Some varieties with higher ornamental value such as: the flower core heavy petals of the water lily Nymphaea 'King of Siam', the water lily Nymphaea 'Foxfire' and the like are formed by the stamen, the flower is long in flowering time, no pollen exists, and the flower core heavy petals are very suitable for being used as cut flowers and have large market demand. However, the varieties can not meet the demands of the market for variety of the cut water lily flowers. Therefore, if the wide-tropical water lily varieties with full petals can be cultivated, the wide-tropical water lily cultivation method has important significance for the development of the water lily industry.
Disclosure of Invention
In order to cultivate the new variety of the full-petaline water lily, the inventor finally discovers that the nine-product water lily is used as a female parent and the blue star water lily is used as a male parent through many years of hybridization breeding and hybridization combination tests, so that the new variety of the full-petaline water lily can be stably created, and the test has operability and repeatability and is worthy of popularization and application.
The technical scheme of the invention is as follows:
a hybridization breeding method of full-petal wide-tropical water lily uses Jiupin lotus as female parent and blue star water lily as male parent to carry out hybridization to obtain a new hybridization variety.
The Jiupin lotus has nine major colors of gold, yellow, purple, blue, red, tea, green, red, white and the like, and the preferred embodiment of the invention is the Jiupin lotus.
The preferable scheme is as follows: a hybridization breeding method of full-petal wide-tropical water lily comprises the following steps:
A. before flowers of the male parent and the female parent are opened, bagging and isolation are carried out;
B. taking stigma fluid in the morning of the flowering day of the female parent;
C. mixing pollen of male parent and stigma fluid of female parent, and pollinating after mixing uniformly; sucking the female parent pistil stigma liquid by a suction pipe during pollination, adding pollination liquid immediately, and continuously bagging and isolating after pollination is finished;
D. after pollination, the fruits are harvested to obtain seeds, and the seeds are preserved for standby.
The preferable scheme is as follows: the mixing mass ratio of the pollen to the female parent stigma liquid is 1:3-5.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a hybridization breeding method of full-petal wide-tropical water lily, which breeds a new full-petal wide-tropical water lily variety, which has obvious differences with parental parents in flower color, flower diameter, re-petal property, leaf diameter and the like, has higher ornamental value, is very suitable for being used as cut flowers, can fill the blank of the new full-petal water lily variety in China, has good economic value, and is worthy of popularization and application.
Drawings
Fig. 1: the invention relates to a crossbreeding process.
Detailed Description
The present invention will be further described with reference to specific examples and drawings for a better understanding of the technical contents of the present invention to those skilled in the art.
Experimental example hybridization breeding of full-petal wide-tropical water lily
The specific breeding process is as follows:
(1) Determination of breeding goals
The breeding aim is to create a new variety of the full-petal cut flower type wide-tropical hybrid water lily.
(2) Selection of parents
And (3) female parent: purple nine-product lotus
Name: purple nine-grade product
The school name: n. 'hybrid'
The characteristics are as follows: flower blue-purple and oval-shaped green leaves.
Habit: light preference, heat preference, aversion to cold.
Reproduction: and (5) seed reproduction.
Belongs to wide-band water lily, has strong hardening capacity and good cut flower water lily female parent.
The male parent is provided with drug-extension water lily, blue bird water lily, blue star water lily, small flower water lily, mineata water lily and the like, and the characteristics of the male parent water lily are as follows:
A. indian drug-prolonging water lily
The school name: n. nochali
The characteristics are as follows: blue-purple flowers, emergent aquatic products, aromatic, oval leaves and green.
Habit: light preference and heat preference.
Reproduction: and (5) seed reproduction.
B. Nymphaea caerulea (L.) Roxb
The school name: n. color
The characteristics are as follows: small variety, bluish violet, nearly circular green leaf.
Habit: light preference, heat preference, aversion to cold.
Reproduction: seed ball breeding.
C. Blue bird water lily
The school name: n.edorado
Characteristics: the characteristics are as follows: yellow flowers, large, green leaves, oval, and even small spots.
Habit: light preference, heat preference, aversion to cold.
Reproduction: seed ball breeding.
D. Mineata water lily
The school name: n.minuta
The characteristics are as follows: microminiature variety, strong setting ability, green and oval leaves and white pollen.
Habit: light preference, heat preference and cold resistance.
Reproduction: and (5) seed reproduction.
E. Flos Nymphaeae
The school name: n.micrantha
The characteristics are as follows: blue-violet, white spots, emergent aquatic products, radial, oval and green leaves.
Habit: preference for heat and preference for strong light.
Reproduction: and (5) fetal growth and reproduction.
F. Egypt blue water lily
The school name: n. caemulea Savigny
The characteristics are as follows: flower blue, star-shaped, strong in setting ability, green on the front of the leaf, nearly circular, and purple black spots on the back. Habit: light preference, heat preference, aversion to cold.
Reproduction: and (5) seed reproduction.
(3) Hybridization breeding process
A. The day before flowers of the male parent and the female parent are opened, bagging and isolation are carried out;
B. the stigma fluid is taken in the morning of the female parent on the day of flowering.
C. And (3) taking pollen of the male parent which is opened the next day, mixing the pollen and the stigma liquid of the female parent (the mass ratio is 1:5), and pollinating after uniformly mixing. When pollinating, sucking the female parent pistil stigma liquid by using a suction pipe, adding the pollinating liquid immediately, and continuously bagging and isolating after the pollinating is finished, so that 100 flowers are pollinated together.
D. After pollination for about 30 days, the fruits are harvested to obtain seeds, and the seeds are stored in water for later use.
E. And sowing the harvested seeds at a proper temperature and in a proper season to obtain cross subgenera hybrid offspring plants. And (5) carrying out character investigation after the plants bloom. The results were as follows:
TABLE 1
The result shows that only the offspring hybridized by using the Jiupin lotus as a female parent and the blue star lotus as a male parent can generate the wide tropical lotus with full petal. Repeated experiments prove that the proportion of the full-petal variety obtained by cross breeding each time reaches more than 60 percent.
The plants of the full-petal variety are reserved, the plant can not be bred, and the multi-generation asexual propagation can be carried out.
Example 1 Cross breeding method of full-petal wide-tropical water lily
Takes nine-product lotus as female parent and blue star lotus as male parent
A. The day before flowers of the male parent and the female parent are opened, bagging and isolation are carried out;
B. taking stigma fluid in the morning of the flowering day of the female parent;
C. and (3) taking pollen of the male parent which is opened the next day, mixing the pollen and the stigma liquid of the female parent (the mass ratio is 1:3), and pollinating after uniformly mixing. And sucking the female parent pistil stigma liquid by using a suction pipe during pollination, adding the pollination liquid immediately, and continuously bagging and isolating after pollination is finished.
D. After pollination for about 30 days, the fruits are harvested to obtain seeds, and the seeds are stored in water for later use.
E. And sowing the harvested seeds at a proper temperature and in a proper season to obtain a new full-petal wide-tropical water lily variety. And (5) carrying out character investigation after the plants bloom.
TABLE 2
Example 2 Cross breeding method of full-petal wide-tropical water lily
Takes blue nine-product lotus as female parent and blue star lotus as male parent
A. The day before flowers of the male parent and the female parent are opened, bagging and isolation are carried out;
B. taking stigma fluid in the morning of the flowering day of the female parent;
C. and (3) taking pollen of the male parent which is opened the next day, mixing the pollen and the stigma liquid of the female parent (the mass ratio is 1:3), and pollinating after uniformly mixing. And sucking the female parent pistil stigma liquid by using a suction pipe during pollination, adding the pollination liquid immediately, and continuously bagging and isolating after pollination is finished.
D. After pollination for about 30 days, the fruits are harvested to obtain seeds, and the seeds are stored in water for later use.
E. The seeds which are harvested by sowing at proper temperature and season can obtain a new variety of the wide-leaf tropical water lily. And (5) carrying out character investigation after the plants bloom.
In other embodiments of the invention, other line of septemloba may also be selected as the female parent.
The above embodiments are only examples of the present invention, and the present invention is not limited thereto, but any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (4)
1. A hybridization breeding method of full-petal wide-tropical water lily is characterized in that nine-product lotus is used as a female parent, blue star lotus is used as a male parent, hybridization is carried out, and full-petal wide-tropical water lily is obtained through screening.
2. The cross breeding method of full-petal wide-tropical water lily according to claim 1, wherein the nine-item lotus is nine-item lotus.
3. The cross breeding method of the full-petal wide-tropical water lily according to claim 1, which is characterized by comprising the following steps:
A. before flowers of the male parent and the female parent are opened, bagging and isolation are carried out;
B. taking stigma fluid in the morning of the flowering day of the female parent;
C. mixing pollen of male parent and stigma fluid of female parent, and pollinating after mixing uniformly; sucking the female parent pistil stigma liquid by a suction pipe during pollination, adding pollination liquid immediately, and continuously bagging and isolating after pollination is finished;
D. after pollination, the fruits are harvested to obtain seeds, and the seeds are preserved for standby.
4. The hybridization breeding method of the full-petal wide-tropical water lily according to claim 3, wherein the mixing mass ratio of pollen to female parent stigma fluid is 1:3-5.
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| CN116267589B true CN116267589B (en) | 2024-01-26 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935075A (en) * | 2018-08-02 | 2018-12-07 | 广西壮族自治区农业科学院花卉研究所 | A method of improving water lily hybridization pollination success rate |
| CN112106652A (en) * | 2020-09-15 | 2020-12-22 | 江苏开放大学（江苏城市职业学院） | Method for screening good variety of fragrant lotus flower fragrance |
| CN113100055A (en) * | 2021-04-28 | 2021-07-13 | 金华市农业科学研究院(浙江省农业机械研究院) | Breeding method of cold-resistant cut flower type water lily across subgenus Australia |
| CN113832254A (en) * | 2021-11-12 | 2021-12-24 | 广西壮族自治区农业科学院 | SSR primer pair of water lily, synthetic method and application |
| CN114431138A (en) * | 2022-02-28 | 2022-05-06 | 中国热带农业科学院热带作物品种资源研究所 | Crossbreeding method and application of tropical water lily |
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- 2023-04-28 CN CN202310492162.0A patent/CN116267589B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935075A (en) * | 2018-08-02 | 2018-12-07 | 广西壮族自治区农业科学院花卉研究所 | A method of improving water lily hybridization pollination success rate |
| CN112106652A (en) * | 2020-09-15 | 2020-12-22 | 江苏开放大学（江苏城市职业学院） | Method for screening good variety of fragrant lotus flower fragrance |
| CN113100055A (en) * | 2021-04-28 | 2021-07-13 | 金华市农业科学研究院(浙江省农业机械研究院) | Breeding method of cold-resistant cut flower type water lily across subgenus Australia |
| CN113832254A (en) * | 2021-11-12 | 2021-12-24 | 广西壮族自治区农业科学院 | SSR primer pair of water lily, synthetic method and application |
| CN114431138A (en) * | 2022-02-28 | 2022-05-06 | 中国热带农业科学院热带作物品种资源研究所 | Crossbreeding method and application of tropical water lily |
Non-Patent Citations (1)
| Title |
|---|
| "49份睡莲资源表型多样性分析及综合评价";苏群等;《西南农业学报》;第32卷(第11期);第2670-2681页 * |
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