CN108291870B - 用于确定样本介质的波长相关折射率的光显微镜和方法 - Google Patents

用于确定样本介质的波长相关折射率的光显微镜和方法 Download PDF

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CN108291870B
CN108291870B CN201680064947.6A CN201680064947A CN108291870B CN 108291870 B CN108291870 B CN 108291870B CN 201680064947 A CN201680064947 A CN 201680064947A CN 108291870 B CN108291870 B CN 108291870B
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sample
light
medium
refractive index
illumination light
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CN108291870A (zh
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安妮特·贝格特
赫尔穆特·利珀特
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/4133—Refractometers, e.g. differential
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Mathematical Physics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CN201680064947.6A 2015-11-09 2016-10-11 用于确定样本介质的波长相关折射率的光显微镜和方法 Active CN108291870B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102015119258.6A DE102015119258A1 (de) 2015-11-09 2015-11-09 Lichtmikroskop und Verfahren zum Bestimmen einer wellenlängenabhängigen Brechzahl eines Probenmediums
DE102015119258.6 2015-11-09
PCT/EP2016/074313 WO2017080737A1 (de) 2015-11-09 2016-10-11 Lichtmikroskop und verfahren zum bestimmen einer wellenlängenabhängigen brechzahl eines probenmediums

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Publication Number Publication Date
CN108291870A CN108291870A (zh) 2018-07-17
CN108291870B true CN108291870B (zh) 2021-06-18

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CN201680064947.6A Active CN108291870B (zh) 2015-11-09 2016-10-11 用于确定样本介质的波长相关折射率的光显微镜和方法

Country Status (6)

Country Link
US (1) US10684221B2 (enExample)
EP (1) EP3374755B1 (enExample)
JP (1) JP6886464B2 (enExample)
CN (1) CN108291870B (enExample)
DE (1) DE102015119258A1 (enExample)
WO (1) WO2017080737A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102015119258A1 (de) 2015-11-09 2017-05-11 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zum Bestimmen einer wellenlängenabhängigen Brechzahl eines Probenmediums
DE102017223787B3 (de) 2017-12-22 2019-05-09 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zur Bestimmung der Brechzahl eines Probenmediums, nichtflüchtiges computerlesbares Speichermedium und Mikroskop
DE102018206486A1 (de) 2018-03-16 2019-09-19 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zum Manipulieren eines Strahlenganges in einem Mikroskop, Verfahren zur Aufnahme von Bilderstapeln in einem Mikroskop und nichtflüchtiges computerlesbares Speichermedium
CN113406046A (zh) * 2021-05-17 2021-09-17 南方医科大学 一种近红外光片显微镜

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087182A (en) 1998-08-27 2000-07-11 Abbott Laboratories Reagentless analysis of biological samples
DE10033269B4 (de) * 2000-07-10 2010-07-01 Leica Microsystems Cms Gmbh Vorrichtung zum Einkoppeln von Licht mindestens einer Wellenlänge einer Laserlichtquelle in ein konfokales Rastermikroskop
JP4316818B2 (ja) * 2001-03-01 2009-08-19 大塚電子株式会社 光散乱測定プローブ
US7006219B2 (en) * 2003-05-20 2006-02-28 Technology Innovations, Llc Biological imager
US7248361B2 (en) * 2005-12-22 2007-07-24 Palo Alto Research Center Incorporated Fluorescence reader based on anti-resonant waveguide excitation
JP2007179753A (ja) * 2005-12-27 2007-07-12 Hitachi High-Technologies Corp 走査透過電子顕微鏡、及び収差測定方法
US20070231787A1 (en) * 2006-04-04 2007-10-04 Voelker Mark A Methods and devices for imaging and manipulating biological samples
CN100494984C (zh) * 2007-07-06 2009-06-03 云南大学 精确测量微量液体折射率的一种方法
CN101701912A (zh) * 2009-11-16 2010-05-05 云南大学 一种无损测量透明毛细管管壁折射率的方法及装置
DE102011081192B4 (de) * 2011-08-18 2016-03-24 Carl Zeiss Ag Mikroskopie zum Ermitteln einer Angabe über eine Brechzahl im Inneren mindestens eines Teils einer Probe
WO2013053454A1 (de) * 2011-10-11 2013-04-18 Carl Zeiss Microscopy Gmbh Mikroskop und verfahren zur spim mikroskopie
DE102012020240A1 (de) * 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur SPIM Mikroskopie
JP6086366B2 (ja) * 2013-04-05 2017-03-01 国立研究開発法人理化学研究所 顕微鏡、焦準器具、流体保持器具、及び光学ユニット
WO2015013044A1 (en) * 2013-07-25 2015-01-29 Optimedica Coporation In situ determination of refractive index of materials
CN203455276U (zh) * 2013-09-18 2014-02-26 云南大学 基于非对称液芯柱透镜精确测量液体折射率及液相扩散系数的装置
US10746980B2 (en) * 2014-08-26 2020-08-18 General Electric Company Calibration of microscopy systems
DE102015119258A1 (de) 2015-11-09 2017-05-11 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zum Bestimmen einer wellenlängenabhängigen Brechzahl eines Probenmediums

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Publication number Publication date
JP2018533014A (ja) 2018-11-08
US20180306714A1 (en) 2018-10-25
CN108291870A (zh) 2018-07-17
US10684221B2 (en) 2020-06-16
JP6886464B2 (ja) 2021-06-16
DE102015119258A1 (de) 2017-05-11
EP3374755B1 (de) 2019-12-04
WO2017080737A1 (de) 2017-05-18
EP3374755A1 (de) 2018-09-19

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