CN108290935A - 用于制造合成的拖丝蜘蛛丝的组合物和方法 - Google Patents

用于制造合成的拖丝蜘蛛丝的组合物和方法 Download PDF

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Publication number
CN108290935A
CN108290935A CN201680058626.5A CN201680058626A CN108290935A CN 108290935 A CN108290935 A CN 108290935A CN 201680058626 A CN201680058626 A CN 201680058626A CN 108290935 A CN108290935 A CN 108290935A
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S·艾特塔禾
M·史梅
U·盖特
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Weekes Material Sciences Corp
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Weekes Material Sciences Corp
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • C12N15/866Baculoviral vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/14011Baculoviridae
    • C12N2710/14041Use of virus, viral particle or viral elements as a vector
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/14011Baculoviridae
    • C12N2710/14041Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Input From Keyboards Or The Like (AREA)
  • Facsimiles In General (AREA)
  • Control Or Security For Electrophotography (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201680058626.5A 2015-08-10 2016-08-10 用于制造合成的拖丝蜘蛛丝的组合物和方法 Pending CN108290935A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201562203102P 2015-08-10 2015-08-10
US62/203,102 2015-08-10
US201662293880P 2016-02-11 2016-02-11
US62/293,880 2016-02-11
US201662317572P 2016-04-03 2016-04-03
US62/317,572 2016-04-03
PCT/IL2016/050874 WO2017025964A1 (en) 2015-08-10 2016-08-10 Compositions and methods for fabricating synthetic dragline spider silk

Publications (1)

Publication Number Publication Date
CN108290935A true CN108290935A (zh) 2018-07-17

Family

ID=57984356

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680058626.5A Pending CN108290935A (zh) 2015-08-10 2016-08-10 用于制造合成的拖丝蜘蛛丝的组合物和方法

Country Status (10)

Country Link
US (2) US10981959B2 (https=)
EP (1) EP3334754B1 (https=)
JP (1) JP7027312B2 (https=)
KR (1) KR102751752B1 (https=)
CN (1) CN108290935A (https=)
AU (1) AU2016305360B2 (https=)
BR (1) BR112018002839A2 (https=)
CA (1) CA2995156C (https=)
ES (1) ES3003957T3 (https=)
WO (1) WO2017025964A1 (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111132995A (zh) * 2017-09-26 2020-05-08 斯柏博科技有限公司 表面活性大分子的结构化
CN111454961A (zh) * 2020-04-22 2020-07-28 西南大学 蜘蛛MaSps基因及其在制备载药纳米粒子中的应用
CN112080493A (zh) * 2020-09-07 2020-12-15 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
CN113563446A (zh) * 2021-07-22 2021-10-29 浙江超丝生物科技有限公司 一种提高力学机械性能的单一多聚蛋白分子及其应用方法
CN114401980A (zh) * 2019-07-04 2022-04-26 赛威克斯材料科学公司 原核表达系统及其使用方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102751752B1 (ko) 2015-08-10 2025-01-09 시비스 머테리얼 사이언스 리미티드 합성 드래그라인 거미 명주를 제조하기 위한 조성물 및 방법
WO2017138002A1 (en) * 2016-02-11 2017-08-17 Seevix Material Sciences Ltd. Composite materials comprising synthetic dragline spider silk
CN115698049A (zh) * 2020-04-23 2023-02-03 赛威克斯材料科学公司 包括牵引蛛丝的化妆品组合物
KR102623531B1 (ko) * 2023-03-17 2024-01-12 에스케이엔펄스 주식회사 연마패드 및 이의 제조방법
WO2025153700A1 (en) * 2024-01-18 2025-07-24 Amsilk Gmbh Silk polypeptides with improved properties
WO2025198514A1 (en) * 2024-03-21 2025-09-25 Anna Rising Recombinant spider silk proteins

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101018806A (zh) * 2004-07-22 2007-08-15 慕尼黑技术大学 重组蜘蛛丝蛋白
CN101133080A (zh) * 2004-06-25 2008-02-27 慕尼黑技术大学 天然来源的蛋白质及由其制备的材料
CN101253193A (zh) * 2005-08-29 2008-08-27 慕尼黑技术大学 修饰的蜘蛛丝蛋白
CN101395178A (zh) * 2005-12-30 2009-03-25 思百博技术股份公司 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法
US20120022005A1 (en) * 2010-05-24 2012-01-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Synthetic Dragline Spider Silk-Like Proteins

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
US7057023B2 (en) * 2002-01-11 2006-06-06 Nexia Biotechnologies Inc. Methods and apparatus for spinning spider silk protein
WO2003060099A2 (en) * 2002-01-11 2003-07-24 Nexia Biotechnologies, Inc. Methods and apparatus for spinning spider silk protein
US9233067B2 (en) * 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
WO2011113446A1 (en) 2010-03-17 2011-09-22 Amsilk Gmbh Method for production of polypeptide containing fibres
US10072152B2 (en) 2012-09-06 2018-09-11 Amsilk Gmbh Methods for producing high toughness silk fibres
WO2016164923A1 (en) * 2015-04-10 2016-10-13 Lewis Randolph V Spider silk and synthetic polymer fiber blends
KR102751752B1 (ko) 2015-08-10 2025-01-09 시비스 머테리얼 사이언스 리미티드 합성 드래그라인 거미 명주를 제조하기 위한 조성물 및 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101133080A (zh) * 2004-06-25 2008-02-27 慕尼黑技术大学 天然来源的蛋白质及由其制备的材料
CN101018806A (zh) * 2004-07-22 2007-08-15 慕尼黑技术大学 重组蜘蛛丝蛋白
CN101253193A (zh) * 2005-08-29 2008-08-27 慕尼黑技术大学 修饰的蜘蛛丝蛋白
CN101395178A (zh) * 2005-12-30 2009-03-25 思百博技术股份公司 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法
US20120022005A1 (en) * 2010-05-24 2012-01-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Synthetic Dragline Spider Silk-Like Proteins

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111132995A (zh) * 2017-09-26 2020-05-08 斯柏博科技有限公司 表面活性大分子的结构化
CN114401980A (zh) * 2019-07-04 2022-04-26 赛威克斯材料科学公司 原核表达系统及其使用方法
CN111454961A (zh) * 2020-04-22 2020-07-28 西南大学 蜘蛛MaSps基因及其在制备载药纳米粒子中的应用
CN111454961B (zh) * 2020-04-22 2023-03-10 西南大学 蜘蛛MaSps基因及其在制备载药纳米粒子中的应用
CN112080493A (zh) * 2020-09-07 2020-12-15 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
CN113563446A (zh) * 2021-07-22 2021-10-29 浙江超丝生物科技有限公司 一种提高力学机械性能的单一多聚蛋白分子及其应用方法

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JP7027312B2 (ja) 2022-03-01
WO2017025964A1 (en) 2017-02-16
US10981959B2 (en) 2021-04-20
EP3334754C0 (en) 2024-12-18
KR102751752B1 (ko) 2025-01-09
EP3334754B1 (en) 2024-12-18
ES3003957T3 (en) 2025-03-11
AU2016305360A1 (en) 2018-03-29
EP3334754A4 (en) 2019-01-16
EP3334754A1 (en) 2018-06-20
US20190002510A1 (en) 2019-01-03
CA2995156A1 (en) 2017-02-16
KR20180039088A (ko) 2018-04-17
US20210317173A1 (en) 2021-10-14
BR112018002839A2 (pt) 2018-11-06
JP2018531040A (ja) 2018-10-25
CA2995156C (en) 2021-06-01
AU2016305360B2 (en) 2021-11-04

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