BR112018002839A2 - composições e métodos para fabricar seda sintética de fio de teia de aranha - Google Patents

composições e métodos para fabricar seda sintética de fio de teia de aranha

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Publication number
BR112018002839A2
BR112018002839A2 BR112018002839A BR112018002839A BR112018002839A2 BR 112018002839 A2 BR112018002839 A2 BR 112018002839A2 BR 112018002839 A BR112018002839 A BR 112018002839A BR 112018002839 A BR112018002839 A BR 112018002839A BR 112018002839 A2 BR112018002839 A2 BR 112018002839A2
Authority
BR
Brazil
Prior art keywords
compositions
cobweb
yarn
methods
mixture
Prior art date
Application number
BR112018002839A
Other languages
English (en)
Inventor
Shimel Meni
Ittah Shmulik
Gat Uri
Original Assignee
Seevix Mat Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seevix Mat Sciences Ltd filed Critical Seevix Mat Sciences Ltd
Publication of BR112018002839A2 publication Critical patent/BR112018002839A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

são proporcionadas composições compreendendo uma mistura de proteínas derivadas de masp, ácidos nucleicos que codificam as mesmas e método para a preparação de seda sintética de fio de teia de aranha. as composições da invenção compreendem uma mistura de proteínas de peso molecular diferente, em que cada proteína da referida mistura compreende, de forma independente, repetições múltiplas de uma região repetitiva de uma proteína masp (de espidroína de ampulácea maior) ou um homólogo, variante, derivado ou fragmento funcional da mesma.
BR112018002839A 2015-08-10 2016-08-10 composições e métodos para fabricar seda sintética de fio de teia de aranha BR112018002839A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562203102P 2015-08-10 2015-08-10
US201662293880P 2016-02-11 2016-02-11
US201662317572P 2016-04-03 2016-04-03
PCT/IL2016/050874 WO2017025964A1 (en) 2015-08-10 2016-08-10 Compositions and methods for fabricating synthetic dragline spider silk

Publications (1)

Publication Number Publication Date
BR112018002839A2 true BR112018002839A2 (pt) 2018-11-06

Family

ID=57984356

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018002839A BR112018002839A2 (pt) 2015-08-10 2016-08-10 composições e métodos para fabricar seda sintética de fio de teia de aranha

Country Status (9)

Country Link
US (2) US10981959B2 (pt)
EP (1) EP3334754A4 (pt)
JP (1) JP7027312B2 (pt)
KR (1) KR20180039088A (pt)
CN (1) CN108290935A (pt)
AU (1) AU2016305360B2 (pt)
BR (1) BR112018002839A2 (pt)
CA (1) CA2995156C (pt)
WO (1) WO2017025964A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108290935A (zh) 2015-08-10 2018-07-17 赛威克斯材料科学公司 用于制造合成的拖丝蜘蛛丝的组合物和方法
EP4375403A2 (en) 2016-02-11 2024-05-29 Seevix Material Sciences Ltd. Composite materials comprising synthetic dragline spider silk
CA3075225A1 (en) * 2017-09-26 2019-04-04 Spiber Technologies Ab Structuring of surface-active macromolecules
EP3994157A4 (en) * 2019-07-04 2023-08-09 Seevix Material Sciences Ltd. PROKARYOTIC EXPRESSION SYSTEM AND METHODS OF USE THEREOF
CN111454961B (zh) * 2020-04-22 2023-03-10 西南大学 蜘蛛MaSps基因及其在制备载药纳米粒子中的应用
BR112022020937A2 (pt) * 2020-04-23 2022-12-20 Seevix Mat Sciences Ltd Composições cosméticas compreendendo seda de aranha dragon sintética.
CN112080493B (zh) * 2020-09-07 2021-12-28 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
CN113563446A (zh) * 2021-07-22 2021-10-29 浙江超丝生物科技有限公司 一种提高力学机械性能的单一多聚蛋白分子及其应用方法
KR102623531B1 (ko) * 2023-03-17 2024-01-12 에스케이엔펄스 주식회사 연마패드 및 이의 제조방법

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003060099A2 (en) * 2002-01-11 2003-07-24 Nexia Biotechnologies, Inc. Methods and apparatus for spinning spider silk protein
US7057023B2 (en) * 2002-01-11 2006-06-06 Nexia Biotechnologies Inc. Methods and apparatus for spinning spider silk protein
EP1609801A1 (en) * 2004-06-25 2005-12-28 Technische Universität München Proteins of natural origin and materials made therefrom
US8030024B2 (en) 2004-06-25 2011-10-04 Amsilk Gmbh Synthesis of spider dragline and/or flagelliform proteins
CN102532295B (zh) * 2004-07-22 2014-10-15 Am丝绸有限责任公司 重组蜘蛛丝蛋白
EP1919938B1 (en) 2005-08-29 2012-06-06 AMSilk GmbH Modified spider silk proteins
CN101395178B (zh) * 2005-12-30 2013-05-01 思百博技术股份公司 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法
US9233067B2 (en) * 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
WO2011113446A1 (en) 2010-03-17 2011-09-22 Amsilk Gmbh Method for production of polypeptide containing fibres
US8461301B2 (en) * 2010-05-24 2013-06-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Synthetic dragline spider silk-like proteins
JP6450680B2 (ja) 2012-09-06 2019-01-09 アーエムシルク ゲーエムベーハー 高靱性シルク繊維を作製する方法
US20160298265A1 (en) * 2015-04-10 2016-10-13 Utah State University Spider silk and synthetic polymer fiber blends
CN108290935A (zh) 2015-08-10 2018-07-17 赛威克斯材料科学公司 用于制造合成的拖丝蜘蛛丝的组合物和方法

Also Published As

Publication number Publication date
CA2995156C (en) 2021-06-01
WO2017025964A1 (en) 2017-02-16
AU2016305360B2 (en) 2021-11-04
AU2016305360A1 (en) 2018-03-29
US20210317173A1 (en) 2021-10-14
CN108290935A (zh) 2018-07-17
CA2995156A1 (en) 2017-02-16
JP7027312B2 (ja) 2022-03-01
US10981959B2 (en) 2021-04-20
US20190002510A1 (en) 2019-01-03
KR20180039088A (ko) 2018-04-17
EP3334754A4 (en) 2019-01-16
EP3334754A1 (en) 2018-06-20
JP2018531040A (ja) 2018-10-25

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