JP7027312B2 - 合成ドラグラインスパイダーシルクを製作するための組成物および方法 - Google Patents

合成ドラグラインスパイダーシルクを製作するための組成物および方法 Download PDF

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JP7027312B2
JP7027312B2 JP2018527035A JP2018527035A JP7027312B2 JP 7027312 B2 JP7027312 B2 JP 7027312B2 JP 2018527035 A JP2018527035 A JP 2018527035A JP 2018527035 A JP2018527035 A JP 2018527035A JP 7027312 B2 JP7027312 B2 JP 7027312B2
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protein
amino acid
proteins
seq
sequence
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JP2018531040A6 (ja
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JP2018531040A (ja
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イッタ，シュムリク
シメル，メニ
ガット，ウリ
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シービックス マテリアル サイエンシーズ リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vectore

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Input From Keyboards Or The Like (AREA)
  • Facsimiles In General (AREA)
  • Control Or Security For Electrophotography (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2018527035A 2015-08-10 2016-08-10 合成ドラグラインスパイダーシルクを製作するための組成物および方法 Active JP7027312B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201562203102P 2015-08-10 2015-08-10
US62/203,102 2015-08-10
US201662293880P 2016-02-11 2016-02-11
US62/293,880 2016-02-11
US201662317572P 2016-04-03 2016-04-03
US62/317,572 2016-04-03
PCT/IL2016/050874 WO2017025964A1 (en) 2015-08-10 2016-08-10 Compositions and methods for fabricating synthetic dragline spider silk

Publications (4)

Publication Number Publication Date
JP2018531040A JP2018531040A (ja) 2018-10-25
JP2018531040A6 JP2018531040A6 (ja) 2018-12-13
JP2018531040A5 JP2018531040A5 (https=) 2019-09-19
JP7027312B2 true JP7027312B2 (ja) 2022-03-01

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ID=57984356

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JP2018527035A Active JP7027312B2 (ja) 2015-08-10 2016-08-10 合成ドラグラインスパイダーシルクを製作するための組成物および方法

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Country Link
US (2) US10981959B2 (https=)
EP (1) EP3334754B1 (https=)
JP (1) JP7027312B2 (https=)
KR (1) KR102751752B1 (https=)
CN (1) CN108290935A (https=)
AU (1) AU2016305360B2 (https=)
BR (1) BR112018002839A2 (https=)
CA (1) CA2995156C (https=)
ES (1) ES3003957T3 (https=)
WO (1) WO2017025964A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102751752B1 (ko) 2015-08-10 2025-01-09 시비스 머테리얼 사이언스 리미티드 합성 드래그라인 거미 명주를 제조하기 위한 조성물 및 방법
WO2017138002A1 (en) * 2016-02-11 2017-08-17 Seevix Material Sciences Ltd. Composite materials comprising synthetic dragline spider silk
EP3688017A1 (en) * 2017-09-26 2020-08-05 Spiber Technologies AB Structuring of surface-active macromolecules
JP2022540563A (ja) * 2019-07-04 2022-09-16 シービックス マテリアル サイエンシーズ リミテッド 原核生物発現システムおよびその使用方法
CN111454961B (zh) * 2020-04-22 2023-03-10 西南大学 蜘蛛MaSps基因及其在制备载药纳米粒子中的应用
CN115698049A (zh) * 2020-04-23 2023-02-03 赛威克斯材料科学公司 包括牵引蛛丝的化妆品组合物
CN112080493B (zh) * 2020-09-07 2021-12-28 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
CN113563446A (zh) * 2021-07-22 2021-10-29 浙江超丝生物科技有限公司 一种提高力学机械性能的单一多聚蛋白分子及其应用方法
KR102623531B1 (ko) * 2023-03-17 2024-01-12 에스케이엔펄스 주식회사 연마패드 및 이의 제조방법
WO2025153700A1 (en) * 2024-01-18 2025-07-24 Amsilk Gmbh Silk polypeptides with improved properties
WO2025198514A1 (en) * 2024-03-21 2025-09-25 Anna Rising Recombinant spider silk proteins

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050054830A1 (en) 2002-01-11 2005-03-10 Shafiul Islam Methods and apparatus for spinning spider silk protein
JP2008507260A (ja) 2004-06-25 2008-03-13 テヒニシェ ウニヴェルズィテート ミュンヘン 天然起源のタンパク質およびそれから作られる材料
US20120022005A1 (en) 2010-05-24 2012-01-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Synthetic Dragline Spider Silk-Like Proteins
WO2014037453A1 (en) 2012-09-06 2014-03-13 Amsilk Gmbh Methods for producing high toughness silk fibres

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7057023B2 (en) * 2002-01-11 2006-06-06 Nexia Biotechnologies Inc. Methods and apparatus for spinning spider silk protein
CN101018806B (zh) 2004-07-22 2014-05-07 Am丝绸有限责任公司 重组蜘蛛丝蛋白
AU2006286753B2 (en) 2005-08-29 2011-08-18 Amsilk Gmbh Modified spider silk proteins
DK1976868T3 (da) 2005-12-30 2015-04-20 Spiber Technologies Ab Edderkoppesilke-proteiner og fremgangsmåde til fremstilling af edderkoppesilke-proteiner
US9233067B2 (en) * 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
WO2011113446A1 (en) 2010-03-17 2011-09-22 Amsilk Gmbh Method for production of polypeptide containing fibres
WO2016164923A1 (en) * 2015-04-10 2016-10-13 Lewis Randolph V Spider silk and synthetic polymer fiber blends
KR102751752B1 (ko) 2015-08-10 2025-01-09 시비스 머테리얼 사이언스 리미티드 합성 드래그라인 거미 명주를 제조하기 위한 조성물 및 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050054830A1 (en) 2002-01-11 2005-03-10 Shafiul Islam Methods and apparatus for spinning spider silk protein
JP2008507260A (ja) 2004-06-25 2008-03-13 テヒニシェ ウニヴェルズィテート ミュンヘン 天然起源のタンパク質およびそれから作られる材料
US20120022005A1 (en) 2010-05-24 2012-01-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Synthetic Dragline Spider Silk-Like Proteins
WO2014037453A1 (en) 2012-09-06 2014-03-13 Amsilk Gmbh Methods for producing high toughness silk fibres

Also Published As

Publication number Publication date
WO2017025964A1 (en) 2017-02-16
US10981959B2 (en) 2021-04-20
EP3334754C0 (en) 2024-12-18
KR102751752B1 (ko) 2025-01-09
EP3334754B1 (en) 2024-12-18
ES3003957T3 (en) 2025-03-11
AU2016305360A1 (en) 2018-03-29
EP3334754A4 (en) 2019-01-16
EP3334754A1 (en) 2018-06-20
US20190002510A1 (en) 2019-01-03
CA2995156A1 (en) 2017-02-16
CN108290935A (zh) 2018-07-17
KR20180039088A (ko) 2018-04-17
US20210317173A1 (en) 2021-10-14
BR112018002839A2 (pt) 2018-11-06
JP2018531040A (ja) 2018-10-25
CA2995156C (en) 2021-06-01
AU2016305360B2 (en) 2021-11-04

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