CN108260800A - The technique for preparing Lactulose liquid using whey - Google Patents
The technique for preparing Lactulose liquid using whey Download PDFInfo
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- CN108260800A CN108260800A CN201611266803.7A CN201611266803A CN108260800A CN 108260800 A CN108260800 A CN 108260800A CN 201611266803 A CN201611266803 A CN 201611266803A CN 108260800 A CN108260800 A CN 108260800A
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Abstract
The invention belongs to sugar from technical field of purification, the technique that Lactulose liquid is prepared using whey is disclosed, is included the following steps：Step 1）Prepare adsorbent, step 2）Heating, centrifugation and adsorption treatment, step 3）Nanofiltration, step 4）Concentration, crystallization and isomerization.The technological operation is simple, of low cost, and the product purity of preparation is high.
Description
Technical field
The invention belongs to sugared preparing technical fields, and in particular to the technique for preparing Lactulose liquid using whey.
Background technology
Whey is often referred to the byproduct caused by during milk or goat milk production cheese.Whey is produced in cheese
In the process, milk condensing is into greeny, the translucent liquid isolated after ziega.Whey Direct spraying be dried to powder or
Concentrate is made, can also be further processed, is processed into other whey products, such as desalted whey powder, low-lactose whey powder, high lactose
Whey powder, lactose, Whey Protein Concentrate and whey protein isolate etc..
It is that raw material warp is heated or is made to carry out isomerization under catalysts conditions with sodium hydroxide that isomerizing lactose, which uses lactose,
It acts on and obtains.Isomerizing lactose is currently on the market existed with two kinds of dosage forms, isomerizing lactose powder and Lactulose liquid.It is different
Structure lactose liquid is colourless to amber liquid.Sugariness is 0.6 times of sucrose, the reason is that containing part in Lactulose liquid
Fructose and galactolipin that the lactose of non-isomerization, hydrolysis generate etc..Lactulose liquid is also referred to as lactulose mouth in medicine field
Liquid is taken, is the drug for specifically treating constipation, hepatic encephalopathy and hepatic coma.
Isomerizing lactose is notable to health function.Beneficial bacterium is Bifidobacterium in human body intestinal canal, and isomerizing lactose is
The best sugar source of bifidobacterium growth, it is not decomposed in small enteral, and moving on to big enteral can be utilized by all Bifidobacteriums, makes double
Discrimination bacillus increases growth that is dominant, inhibiting putrefactivebacteria and pathogen, to change intestinal flora, keep enteron aisle normal function,
It prevents and cures diseases anti-aging etc. and to play an important role.Isomerizing lactose is prevented treating constipation also very notable with anti-cancer efficacy.Enteral Bifidobacterium
Proliferation, pH value reduce, promote the wriggling of intestines, excrement is made to soften, defecation quantity and number can increase, and enteral can be made easily to make
The residence time shortens the dud grown into spoilage organisms in the intestine, plays the role of preventing intestinal cancer.With breast-fed babies' ratio
The intestinal bifidobacteria of the baby of artificial feeding has comparative advantage（Account for more than 90% total bacterium）, the former is stronger than the latter resistance, suffers from
Sick rate is low, and growth and development is good, because there is the factor for promoting baby's enteral bifidobacterium growth in breast milk.0. is such as added in milk powder
5% isomerizing lactose, artificially feeding infant can increase baby enteral Bifidobacterium ratio, give birth to children close to breast milk, resistance
Enhancing, enhancing development reduce illness rate.Isomerizing lactose can be widely used in health food, can be added to milk beverage, carbon
Make diet remedy diet in the food such as acid beverage, fruit drink, candy, milk powder, also can individually make medical supplies.
Isomerizing lactose is made by lactose isomerization, due to lactose higher price so that the price of isomerizing lactose occupies high
Under not, we can be produced by reducing lactose and improve the profit of corresponding enterprise originally.From whey extract lactose only from
In laboratory stage, the defects of it is not high to be primarily present purity, complex for operation step, and cost is higher.How to make improvements is
Our technical issues that need to address.
Invention content
The defects of in order to overcome the prior art, the object of the present invention is to provide the works that sharp bovine whey prepares Lactulose liquid
Skill, the technological operation is simple, of low cost, and the product purity of preparation is high.
The present invention adopts the following technical scheme that realization：
The technique that Lactulose liquid is prepared using whey, is included the following steps：Step 1）Prepare adsorbent, step 2）Add
Heat, centrifugation and adsorption treatment, step 3）Nanofiltration, step 4）Concentration, crystallization and isomerization.
Specifically, the technique includes the following steps：
Step 1）Prepare adsorbent：By sepiolite powder, diatomite, turfy soil and organobentonite according to 5:3:2:2 quality
Than being mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is then added
Enter to account for the polyvinyl alcohol water solution of a concentration of 8wt% of acrylic sphere quality 2 percent, the particle that grain size is 0.5mm is made, so
100 DEG C of temperature drying 120min are placed on, places into sintering furnace and is sintered, 800 DEG C of sintering temperature keeps the temperature 90min, takes out, cold
But to room temperature to get；
Step 2）Heating, centrifugation and adsorption treatment：Bovine whey is placed in container, is heated to 70 DEG C, keeps the temperature 5min, then
1000rpm centrifuges 6-10min, collects supernatant, and adsorbent is then added into supernatant, and 300rpm stirs 5-10min, then
90-120min is stood, filtrate is collected in the filtering of 100 mesh screens；
Step 3）Nanofiltration：By filtrate by NF membrane, filtered solution is collected；
Step 4）Concentration, crystallization and isomerization：Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters
Crystallizing tank, control feed temperature are 85-90 DEG C, keep the temperature 60-90min, and separation obtains crystal and mother liquor, and crystal is dried and obtains breast
Sugar；The purifying water dissolution of 10 times of weight is added into lactose, is then heated to 120 DEG C, keeps the temperature 10min, then be cooled to 10 rapidly
DEG C, the sodium hydroxide for accounting for solution quality 0.01% is then added in, then 300rpm stirring 3min are removed by ion exchange resin
Sodium ion is to get Lactulose liquid.
Preferably, the additive amount of the adsorbent accounts for the 0.2% of supernatant quality.
Preferably, the nanofiltration temperature of the NF membrane is 30 DEG C, interception 500Da.
The present invention can also prepare isomerizing lactose using goat milk to isolate lactose.
The invention mainly comprises but be not restricted to that the advantages of the following aspects and progress：
The present invention isolates lactose to prepare isomerizing lactose from whey, greatly reduces cost of material；Lactose of the present invention carries
Take purifying process simple, lactose purity is high, and operability is strong, avoids using multiple ultrafiltration nanofiltration, repeatedly ion exchange resin exchange, weight
Complex crystallization and etc., industrial energy consumption is reduced, alleviates entreprise cost；The present invention has large aperture knot using new adsorbent
Structure, permeability is good, bulk density is small, coefficient of thermal expansion is low, the porosity is high, adsorption capacity and surface area are big, can be effectively
Adsorbed proteins, fat and small molecule inorganic salts etc., mechanical strength is big, it is not easy to destroy, and low raw-material cost；This hair
It is bright that removing protein is gone by protein heat denatured, centrifugation, handled using adsorbent and nanofiltration, effectively remove protein,
Inorganic salts and fatty ingredient substantially increase lactose purity, increase the added value of industry.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, have below in conjunction with the application
Body embodiment more clearly and completely describes the present invention, it is clear that described embodiment is only the application one
Divide embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
All other embodiments obtained under the premise of creative work should all belong to the scope of protection of the invention.
Embodiment 1
The technique that Lactulose liquid is prepared using whey, is included the following steps：
Bovine whey constituent analysis, weight percent：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts
0.7-0.8%, remaining is water.
Prepare adsorbent：By sepiolite powder, diatomite, turfy soil and organobentonite according to 5:3:2:2 mass ratio
It is mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is subsequently added into
The polyvinyl alcohol water solution of a concentration of 8wt% of the 2% of acrylic sphere quality is accounted for, the particle that grain size is 0.5mm is made, is subsequently placed in
100 DEG C of temperature dry 120min, place into sintering furnace and are sintered, and 800 DEG C of sintering temperature keeps the temperature 90min, takes out, is cooled to room
Temperature to get；
Heating, centrifugation and adsorption treatment：Bovine whey is placed in container, is heated to 70 DEG C, keeps the temperature 5min, then 1000rpm
8min is centrifuged, collects supernatant, then addition accounts for supernatant 0.2% into supernatant（w/w）Adsorbent, 300rpm stirring
6min, then stands 90min, and filtrate is collected in the filtering of 100 mesh screens；
Nanofiltration：By filtrate by NF membrane, nanofiltration temperature is 30 DEG C, interception 500Da, collects filtered solution；
Concentration, crystallization and isomerization：Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallization
Tank, control feed temperature are 85 DEG C, keep the temperature 90min, and separation obtains crystal and mother liquor, and crystal is dried and obtains lactose（Lactose is received
Rate is 74.5%）；The purifying water dissolution of 10 times of weight is added into lactose, is then heated to 120 DEG C, keeps the temperature 10min, it is rapid to drop
Then temperature adds in the sodium hydroxide for accounting for solution quality 0.01% to 10 DEG C, then 300rpm stirring 3min pass through amberlite
Fat removes sodium ion to get Lactulose liquid.Product appearance is yellow transparent shape liquid, meets GB8816-88 national standards.
Embodiment 2
The technique that Lactulose liquid is prepared using bovine whey, is included the following steps：
Bovine whey constituent analysis, weight percent：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts
0.7-0.8%, remaining is water.
Prepare adsorbent：By sepiolite powder, diatomite, turfy soil and organobentonite according to 5:3:2:2 mass ratio
It is mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is subsequently added into
The polyvinyl alcohol water solution of a concentration of 8wt% of the 2% of acrylic sphere quality is accounted for, the particle that grain size is 0.5mm is made, is subsequently placed in
100 DEG C of temperature dry 120min, place into sintering furnace and are sintered, and 800 DEG C of sintering temperature keeps the temperature 90min, takes out, is cooled to room
Temperature to get；
Heating, centrifugation and adsorption treatment：Bovine whey is placed in container, is heated to 70 DEG C, keeps the temperature 5min, then 1000rpm
10min is centrifuged, collects supernatant, then addition accounts for supernatant 0.2% into supernatant（w/w）Adsorbent, 300rpm stirring
10min, then stands 120min, and filtrate is collected in the filtering of 100 mesh screens；
Nanofiltration：By filtrate by NF membrane, nanofiltration temperature is 30 DEG C, interception 500Da, collects filtered solution；
Concentration, crystallization and isomerization：Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallization
Tank, control feed temperature are 90 DEG C, keep the temperature 60min, and separation obtains crystal and mother liquor, and crystal is dried and obtains lactose（Lactose is received
Rate is 73.9%）；The purifying water dissolution of 10 times of weight is added into lactose, is then heated to 120 DEG C, keeps the temperature 10min, it is rapid to drop
Then temperature adds in the sodium hydroxide for accounting for solution quality 0.01% to 10 DEG C, then 300rpm stirring 3min pass through amberlite
Fat removes sodium ion to get Lactulose liquid.Product appearance is yellow transparent shape liquid, and product meets GB8816-88 countries
Standard.
Embodiment 3
The obtained lactose constituent detection of the present invention：
Table 1
| Project | Standard QB/T3788-1999 | The embodiment of the present invention 1 | The embodiment of the present invention 2 |
| Organoleptic standard | There are the distinctive sweet taste of lactose, white crystalline | Meet | Meet |
| Lactose content % | 90 | 94.3 | 94.6 |
| Moisture % | 3 | 2.2 | 2.0 |
Embodiment 4
Adsorbent contrast test：
Three groups are set：Respectively test group, control group 1, control group 2.Wherein, test group is embodiment 1；Control group 1 is adopted
By the use of activated carbon as adsorbent, remaining is the same as embodiment 1；Control group 2, using diatomite as adsorbent, remaining is the same as embodiment 1.
Protein content is respectively in different adsorbents treated lactose prod：0.04%, 0.16%, 0.11%；Inorganic salt content is distinguished
For：0.05%, 0.16%, 0.23%.Conclusion：Adsorbent of the present invention is substantially better than existing in terms of adsorbed proteins, inorganic salts and other effects
There is technology often to use adsorbent.
Although the embodiments of the present invention have been disclosed as above, but it is not restricted to listed fortune in specification and embodiment
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily real
Now other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details and embodiment shown and described herein.
Claims (4)
1. preparing the technique of Lactulose liquid using whey, include the following steps：Step 1）Prepare adsorbent, step 2）Add
Heat, centrifugation and adsorption treatment, step 3）Nanofiltration, step 4）Concentration, crystallization and isomerization.
2. technique according to claim 1, which is characterized in that the technique includes the following steps：
Step 1）Prepare adsorbent：By sepiolite powder, diatomite, turfy soil and organobentonite according to 5:3:2:2 quality
Than being mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is then added
Enter to account for the polyvinyl alcohol water solution of a concentration of 8wt% of acrylic sphere quality 2 percent, the particle that grain size is 0.5mm is made, so
100 DEG C of temperature drying 120min are placed on, places into sintering furnace and is sintered, 800 DEG C of sintering temperature keeps the temperature 90min, takes out, cold
But to room temperature to get；
Step 2）Heating, centrifugation and adsorption treatment：Whey is placed in container, is heated to 70 DEG C, keeps the temperature 5min, then
1000rpm centrifuges 6-10min, collects supernatant, and adsorbent is then added into supernatant, and 300rpm stirs 5-10min, then quiet
90-120min is put, filtrate is collected in the filtering of 100 mesh screens；
Step 3）Nanofiltration：By filtrate by NF membrane, filtered solution is collected；
Step 4）Concentration, crystallization and isomerization：By step 3）Gained filtered solution is concentrated into 1/5th of original volume, after concentration
Feed liquid enter crystallizing tank, control feed temperature is 85-90 DEG C, keeps the temperature 60-90min, separation obtains crystal and mother liquor, by crystal
It is dry to obtain lactose；The purifying water dissolution of 10 times of weight is added into lactose, is then heated to 120 DEG C, keeps the temperature 10min, then fast
Speed is cooled to 10 DEG C, then adds in the sodium hydroxide for accounting for solution quality 0.01%, and 300rpm stirring 3min are handed over finally by ion
It changes resin and removes sodium ion to get Lactulose liquid.
3. technique according to claim 1, which is characterized in that the additive amount of the adsorbent accounts for supernatant quality
0.2%。
4. technique according to claim 2, which is characterized in that the nanofiltration temperature of the NF membrane is 30 DEG C, retains molecule
It measures as 500Da.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611266803.7A CN108260800A (en) | 2016-12-31 | 2016-12-31 | The technique for preparing Lactulose liquid using whey |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611266803.7A CN108260800A (en) | 2016-12-31 | 2016-12-31 | The technique for preparing Lactulose liquid using whey |
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| CN108260800A true CN108260800A (en) | 2018-07-10 |
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| CN201611266803.7A Pending CN108260800A (en) | 2016-12-31 | 2016-12-31 | The technique for preparing Lactulose liquid using whey |
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Citations (7)
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| CN88101501A (en) * | 1987-05-13 | 1988-11-30 | 格雷斯公司 | Use plural gel adsorbed proteins from fluid |
| JP2000106863A (en) * | 1998-10-01 | 2000-04-18 | Ngk Insulators Ltd | Method for removing dregs |
| CN1324956A (en) * | 2001-07-10 | 2001-12-05 | 马永明 | Prepn. of lactulose |
| CN101491287A (en) * | 2008-01-24 | 2009-07-29 | 滕国新 | Method for extracting lactose and lactoalbumin from whey and producing formulation milk powder |
| CN104014300A (en) * | 2013-07-29 | 2014-09-03 | 吉林省临江天元催化剂有限公司 | Method for preparing beer stabilizer with filtering assistance function from diatomaceous earth |
| CN104529506A (en) * | 2015-01-16 | 2015-04-22 | 临江北峰硅藻土有限公司 | Preparation method of compound natural porous mineral ceramic microbeads |
| CN105541928A (en) * | 2015-12-16 | 2016-05-04 | 新希望双喜乳业（苏州）有限公司 | Method for extracting high purity lactose from milk whey |
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2016
- 2016-12-31 CN CN201611266803.7A patent/CN108260800A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN88101501A (en) * | 1987-05-13 | 1988-11-30 | 格雷斯公司 | Use plural gel adsorbed proteins from fluid |
| JP2000106863A (en) * | 1998-10-01 | 2000-04-18 | Ngk Insulators Ltd | Method for removing dregs |
| CN1324956A (en) * | 2001-07-10 | 2001-12-05 | 马永明 | Prepn. of lactulose |
| CN101491287A (en) * | 2008-01-24 | 2009-07-29 | 滕国新 | Method for extracting lactose and lactoalbumin from whey and producing formulation milk powder |
| CN104014300A (en) * | 2013-07-29 | 2014-09-03 | 吉林省临江天元催化剂有限公司 | Method for preparing beer stabilizer with filtering assistance function from diatomaceous earth |
| CN104529506A (en) * | 2015-01-16 | 2015-04-22 | 临江北峰硅藻土有限公司 | Preparation method of compound natural porous mineral ceramic microbeads |
| CN105541928A (en) * | 2015-12-16 | 2016-05-04 | 新希望双喜乳业（苏州）有限公司 | Method for extracting high purity lactose from milk whey |
Non-Patent Citations (1)
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| 李涛: "从牛奶乳清液中提取乳糖与异构化乳糖的研究", 《乳品加工》 * |
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