CN108264528A - A kind of method that lactose is isolated and purified from whey - Google Patents
A kind of method that lactose is isolated and purified from whey Download PDFInfo
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- CN108264528A CN108264528A CN201611266808.XA CN201611266808A CN108264528A CN 108264528 A CN108264528 A CN 108264528A CN 201611266808 A CN201611266808 A CN 201611266808A CN 108264528 A CN108264528 A CN 108264528A
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- lactose
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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Abstract
The invention belongs to sugars from technical field of purification, disclose a kind of method that lactose is isolated and purified from whey, including：Whey is placed in container, is heated to 70 75 DEG C, heat preservation is then centrifuged for, and collects supernatant, adsorbent is then added into supernatant, is stirred, is then stood, and is filtered, and collects filtrate；By filtrate by NF membrane, filtered solution is collected；Filtered solution is concentrated, is crystallized, it is dry to obtain.Method for extraction and purification of the present invention is simple, and lactose purity is high, and operability is strong.
Description
Technical field
The invention belongs to sugar from technical field of purification, it is related to a kind of method that lactose is isolated and purified from whey.
Background technology
Whey is often referred to the byproduct caused by during milk or goat milk production cheese.Whey is produced in cheese
In the process, milk condensing is into greeny, the translucent liquid isolated after ziega.Whey Direct spraying be dried to powder or
Concentrate is made, can also be further processed, is processed into other whey products, such as desalted whey powder, low-lactose whey powder, high lactose
Whey powder, lactose, Whey Protein Concentrate and whey protein isolate etc..
Lactose is existed only in nature in the milk of mammal, and a molecule lactose digestion can get a point sub- glucose
With a molecule galactolipin.The major function of lactose be for human body heat supply, the growth and development of children and adult, metabolism,
The synthesis of tissue maintains normal body temperature and physical training, labour work to be required for a large amount of thermal energy, particularly children to sugar
Decomposition digest and absorb it is all more vigorous than adult, lactose be children's intracorporeal organ, nerve, four limbs, muscle, etc. development and activity
Power.The brain cell development of children and the sound of entire nervous system are required for a large amount of lactose, the children within an one full year of life
13 grams or so of sugar is needed per kg body weight per day, another important function of lactose is to promote the lactic acid bacteria in children intestinal numerous
Growth is grown, lactose generates lactic acid under the action of Bacillus acidi lactici streptococcus lactis, a variety of enzymes and certain micro-organisms in enteron aisle, breast
Acid has adjustment protective effect to children's stomach, and intoxicating phenomenon caused by it can inhibit the toxin that enteral abnormal fermentation generates may be used also
Inhibit the breeding of intestinal toxic bacterium.Another effect of lactose is can to promote suction of the children to calcium in the metabolic process of calcium
It receives.Sweet taste in breast is just from lactose, but that sugariness is compared with other carbohydrates is relatively low so the partial eclipse of children will not be caused for lactose.
At present, the lactose of commercialization is synthesized mostly by glucose, and higher price；From whey extract lactose only from
In laboratory stage, the defects of it is not high to be primarily present purity, complex for operation step, and cost is higher.Traditional crystallisation includes：Knot
The experiment flow of crystallization is as follows：Milk → degreasing → acid → whey → heating → neutralization → heating → filtering → evaporation and concentration
→ crystallization → separation mother liquor → wash crystallization → drying → lactose → inspection → packaging → finished product；Its there are complex for operation step,
The defects of lactose purity is not high.Moreover, the lactose difference in selling prices of different purity is very big, lactose life can be improved by improving lactose purity
Produce the profit of enterprise.
Invention content
The defects of in order to overcome the prior art, isolates and purifies lactose the object of the present invention is to provide a kind of from bovine whey
Method, operation is simple and feasible for this method, and industrial energy consumption is low, substantially increases the purity of lactose, complies fully with relevant national standard.
The present invention adopts the following technical scheme that realization：
A kind of method that lactose is isolated and purified from bovine whey, includes the following steps：
Whey is placed in container, is heated to 70-75 DEG C, keeps the temperature 5-10min, then 2000rpm centrifuges 3-5min, collects supernatant
Liquid, then into supernatant, addition accounts for supernatant 0.3-0.5%（w/w）Adsorbent, 300rpm stirring 5min, then stand 60-
90min, 100 mesh screens filtering, collects filtrate；
By filtrate by NF membrane, nanofiltration temperature is 30-35 DEG C, interception 500Da, collects filtered solution；
Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallizing tank, and control feed temperature is 90 DEG C,
30-60min is kept the temperature, separation obtains crystal and mother liquor, and crystal is dried and obtains lactose.
Specifically, the adsorbent is prepared according to following technique：
By sepiolite powder, diatomite, turfy soil and organobentonite according to 3:2:1:1 mass ratio is mixed evenly to obtain
Mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is subsequently added into and is accounted for acrylic sphere quality
The particle that grain size is 1mm is made in the polyvinyl alcohol water solution of 2% a concentration of 8wt%, is subsequently placed in 100 DEG C of temperature dryings
120min is placed into sintering furnace and is sintered, 800 DEG C of sintering temperature, keep the temperature 90min, take out, be cooled to room temperature to get.
Preferably, the whey is bovine whey, and ingredient is according to weight percent：Protein 0.5-0.6%, fatty 0.2-
0.3%, lactose 5.1-5.3%, inorganic salts 0.7-0.8%, remaining is water.
The lactose prepared according to the above-mentioned method being allowed to described in one.
The present invention is it is also an option that goat milk carrys out separate lactose.
The invention mainly comprises but be not restricted to that the advantages of the following aspects and progress：
Extraction and purification process of the present invention is simple, and lactose purity is high, and operability is strong, avoids using multiple ultrafiltration nanofiltration, ion tree
Ester exchange, be repeated several times crystallization and etc., reduce industrial energy consumption, alleviate entreprise cost；The present invention uses new adsorbent,
Has macropore gauge structure, permeability is good, bulk density is small, coefficient of thermal expansion is low, the porosity is high, adsorption capacity and surface area
Greatly, can effectively adsorbed proteins, fat and small molecule inorganic salts etc., mechanical strength is big, it is not easy to destroy, and raw material
It is of low cost；The present invention removes removing protein by protein heat denatured, centrifugation, is handled using adsorbent and nanofiltration, effectively
Protein, inorganic salts and fatty ingredient are eliminated, lactose purity is substantially increased, increases the added value of industry.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, have below in conjunction with the application
Body embodiment more clearly and completely describes the present invention, it is clear that described embodiment is only the application one
Divide embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
All other embodiments obtained under the premise of creative work should all belong to the scope of protection of the invention.
Embodiment 1
A kind of method that lactose is isolated and purified from bovine whey, includes the following steps：
Bovine whey constituent analysis, weight percent：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts
0.7-0.8%, remaining is water.
Bovine whey is placed in container, is heated to 70 DEG C, keeps the temperature 5min, then 2000rpm centrifuges 5min, collects supernatant
Liquid, then into supernatant, addition accounts for supernatant 0.3%（w/w）Adsorbent, 300rpm stirring 5min, then stand 90min,
100 mesh screens filter, and collect filtrate；
By filtrate by NF membrane, nanofiltration temperature is 30 DEG C, interception 500Da, collects filtered solution；
Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallizing tank, and control feed temperature is 90 DEG C,
60min is kept the temperature, separation obtains crystal and mother liquor, and crystal is dried and obtains lactose；Wherein, lactose yield is 75.3%.
The adsorbent is prepared in accordance with the following steps：By sepiolite powder, diatomite, turfy soil and organobentonite
According to 3:2:1:1 mass ratio is mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is put
In comminutor, the polyvinyl alcohol water solution for the 2% a concentration of 8wt% for accounting for acrylic sphere quality is subsequently added into, grain size, which is made, is
The particle of 1mm is subsequently placed in 100 DEG C of temperature drying 120min, places into sintering furnace and be sintered, 800 DEG C of sintering temperature, heat preservation
90min, take out, be cooled to room temperature to get；.
Embodiment 2
A kind of method that lactose is isolated and purified from bovine whey, includes the following steps：
Bovine whey constituent analysis, weight percent：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts
0.7-0.8%, remaining is water.
Bovine whey is placed in container, is heated to 75 DEG C, keeps the temperature 6min, then 2000rpm centrifuges 3min, collects supernatant
Liquid, then into supernatant, addition accounts for supernatant 0.5%（w/w）Adsorbent, 300rpm stirring 5min, then stand 60min,
100 mesh screens filter, and collect filtrate；
By filtrate by NF membrane, nanofiltration temperature is 35 DEG C, interception 500Da, collects filtered solution；
Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallizing tank, and control feed temperature is 90 DEG C,
30min is kept the temperature, separation obtains crystal and mother liquor, and crystal is dried and obtains lactose；Wherein, lactose yield is 74.9%.
The adsorbent is prepared in accordance with the following steps：By sepiolite powder, diatomite, turfy soil and organobentonite
According to 3:2:1:1 mass ratio is mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is put
In comminutor, the polyvinyl alcohol water solution for the 2% a concentration of 8wt% for accounting for acrylic sphere quality is subsequently added into, grain size, which is made, is
The particle of 1mm is subsequently placed in 100 DEG C of temperature drying 120min, places into sintering furnace and be sintered, 800 DEG C of sintering temperature, heat preservation
90min, take out, be cooled to room temperature to get.
Embodiment 3
A kind of method that lactose is isolated and purified from bovine whey, includes the following steps：
Bovine whey constituent analysis, weight percent：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts
0.7-0.8%, remaining is water.
Bovine whey is placed in container, is heated to 70 DEG C, keeps the temperature 10min, then 2000rpm centrifugations 3-5min, in collection
Clear liquid, then into supernatant, addition accounts for supernatant 0.4%（w/w）Adsorbent, 300rpm stirring 5min, then stand
80min, 100 mesh screens filtering, collects filtrate；
By filtrate by NF membrane, nanofiltration temperature is 32 DEG C, interception 500Da, collects filtered solution；
Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallizing tank, and control feed temperature is 90 DEG C,
50min is kept the temperature, separation obtains crystal and mother liquor, and crystal is dried and obtains lactose；Wherein, lactose yield is 78.1%.
The adsorbent is prepared in accordance with the following steps：By sepiolite powder, diatomite, turfy soil and organobentonite
According to 3:2:1:1 mass ratio is mixed evenly to obtain mixture；By mixture and acrylic sphere according to 1:2 volume ratio is put
In comminutor, the polyvinyl alcohol water solution for the 2% a concentration of 8wt% for accounting for acrylic sphere quality is subsequently added into, grain size, which is made, is
The particle of 1mm is subsequently placed in 100 DEG C of temperature drying 120min, places into sintering furnace and be sintered, 800 DEG C of sintering temperature, heat preservation
90min, take out, be cooled to room temperature to get.
Embodiment 4
The lactose constituent detection that the present invention isolates and purifies：
Table 1
| Project | Standard QB/T3788-1999 | The embodiment of the present invention 1 | The embodiment of the present invention 2 | The embodiment of the present invention 3 |
| Organoleptic standard | There are the distinctive sweet taste of lactose, white crystalline | Meet | Meet | Meet |
| Lactose content % | 90 | 94.9 | 95.4 | 95.1 |
| Moisture % | 3 | 2.0 | 2.1 | 2.4 |
Embodiment 5
Adsorbent contrast test：
Three groups are set：Respectively test group, control group 1, control group 2.Wherein, test group is embodiment 1；Control group 1 is adopted
By the use of activated carbon as adsorbent, remaining is the same as embodiment 1；Control group 2, using diatomite as adsorbent, remaining is the same as embodiment 1.
Protein content is respectively in different adsorbents treated product：0.03%, 0.12%, 0.09%；Inorganic salt content is respectively：
0.06%, 0.17%, 0.26%.Conclusion：Using adsorbent of the present invention, adsorbed proteins, inorganic salts and other effects are normal better than the prior art
Use adsorbent.
Although the embodiments of the present invention have been disclosed as above, but it is not restricted to listed fortune in specification and embodiment
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily real
Now other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details and embodiment shown and described herein.
Claims (4)
1. a kind of method that lactose is isolated and purified from whey, includes the following steps：
Whey is placed in container, is heated to 70-75 DEG C, keeps the temperature 5-10min, then 2000rpm centrifuges 3-5min, collects supernatant
Liquid, then into supernatant, addition accounts for supernatant 0.3-0.5%（w/w）Adsorbent, 300rpm stirring 5min, then stand 60-
90min, 100 mesh screens filtering, collects filtrate；
By filtrate by NF membrane, nanofiltration temperature is 30-35 DEG C, molecular cut off 500Da, collects filtered solution；
Filtered solution is concentrated into 1/5th of original volume, the feed liquid after concentration enters crystallizing tank, and control feed temperature is 90 DEG C,
30-60min is kept the temperature, separation obtains crystal and mother liquor, and crystal is dried and obtains lactose.
2. according to the method described in claim 1, it is characterized in that, the adsorbent is prepared according to following technique：
By sepiolite powder, diatomite, turfy soil and organobentonite according to 3:2:1:1 mass ratio is mixed evenly to obtain
Mixture；By mixture and acrylic sphere according to 1:2 volume ratio is placed in comminutor, is subsequently added into and is accounted for acrylic sphere quality
The particle that grain size is 1mm is made in the polyvinyl alcohol water solution of 2% a concentration of 8wt%, is subsequently placed in 100 DEG C of temperature dryings
120min is placed into sintering furnace and is sintered, 800 DEG C of sintering temperature, keep the temperature 90min, take out, be cooled to room temperature to get.
3. according to the method described in claim 1, it is characterized in that, the whey is bovine whey, ingredient is according to weight percent
Than for：Protein 0.5-0.6%, fatty 0.2-0.3%, lactose 5.1-5.3%, inorganic salts 0.7-0.8%, remaining is water.
4. lactose prepared by the method described in one is allowed to according to claim 1-3.
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| CN201611266808.XA CN108264528A (en) | 2016-12-31 | 2016-12-31 | A kind of method that lactose is isolated and purified from whey |
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| CN201611266808.XA CN108264528A (en) | 2016-12-31 | 2016-12-31 | A kind of method that lactose is isolated and purified from whey |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020111980A1 (en) * | 2018-11-30 | 2020-06-04 | Акционерное общество "Молочный комбинат "Ставропольский" | Lactose production method |
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2016
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| CN88101501A (en) * | 1987-05-13 | 1988-11-30 | 格雷斯公司 | Use plural gel adsorbed proteins from fluid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020111980A1 (en) * | 2018-11-30 | 2020-06-04 | Акционерное общество "Молочный комбинат "Ставропольский" | Lactose production method |
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Application publication date: 20180710 |