CN105541928A - Method for extracting high purity lactose from milk whey - Google Patents
Method for extracting high purity lactose from milk whey Download PDFInfo
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- CN105541928A CN105541928A CN201510941201.6A CN201510941201A CN105541928A CN 105541928 A CN105541928 A CN 105541928A CN 201510941201 A CN201510941201 A CN 201510941201A CN 105541928 A CN105541928 A CN 105541928A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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Abstract
The invention discloses a method for extracting high purity lactose from milk whey. The method comprises 1, carrying out primary centrifugation on fresh milk to obtain defatted milk, 2, carrying out microfiltration and secondary centrifugation to obtain milk whey and carrying out ultrafiltration to obtain a first penetrative material treated through a first ultrafiltration film, 3, adding an acetic acid-sodium chloride solution into the first penetrative material and carrying out secondary ultrafiltration to obtain a second penetrative material, 4, dropwisely adding the second penetrative material into a sodium hydroxide solution with a concentration of 0.1-5wt%, carrying out filter pressing to obtain a first retentate, dissolving the first retentate in a hydrochloric acid solution with a concentration of 0.08-1.6wt% and carrying out nanofiltration to obtain a forth penetrative material and 5, gradually adding lactose crystals in the forth penetrative material solution and heating the forth penetrative material solution until the solution is dried so that lactose is obtained. The method solves the technical problem that lactose is not easily extracted and has low extraction purity.
Description
Technical field
The invention belongs to the production technical field of milk-product, particularly a kind of method extracting high purity lactose from milk whey.
Background technology
Whey product belongs to the category of milk-product, is the byproduct of cheesemaking, can produce the cheese of about 1 kilogram with the fresh cow milks of 10 kilograms, and remaining is then whey.The dry-matter of fresh whey is approximately 7%, but includes the nutritive ingredient of nearly half in sweet milk.Fresh in the production process of cheese, newborn most fat and the casein accounting for total protein 80% define the basic boom of cheese grumeleuse, and whey is then the composition beyond cheese grumeleuse.The nutritive ingredient contained in whey is all solvable substantially, as whey-protein.Phosphatide, lactose.Mineral substance and VITAMIN etc.
Fresh whey after pasteurize, then through being dried to powdery, Here it is whey powder.The ratio of other compositions in whey powder except moisture should be consistent with fresh whey.
In the U.S., the whey Direct spraying of nearly 2/3 is dried to whey powder or makes whey concentrated solution.All the other wheys of 1/3 are processed into other whey products further.As desalted whey powder, low-lactose whey powder, high lactose whey powder, lactose, whey protein concentrate and whey isolate protein.
Develop in external dairy industry and used ultra-filtration technique to make " pre-cheese " and whey enriched material.Compared with traditional evaporation concentration, membrane technique can not only reduce the protein denaturation heating and cause, and has clear superiority in product purification.Be dried to powder at the whey Direct spraying of the U.S. about 2/3rds or make enriched material, the whey that its excess-three is divided does further process, be processed into other whey products, as desalted whey powder, low-lactose whey powder, high lactose whey powder, lactose, whey protein concentrate and whey isolate protein etc.
Usual at present the whey convection drying after process is become lactose whey powder, or the purity of lactose powder is not high, as can be seen here, in prior art, not easily from milk, extract highly purified lactose, DNA purity is not high, or extraction cost is large, cannot industrial application.
Summary of the invention
For above-mentioned technical problem, in the present invention, propose a kind of method extracting high purity lactose from milk whey, solve the technical problem that lactose not easily extracts, DNA purity is not high.
In order to realize, according to these objects of the present invention and other advantage, providing a kind of method extracting high purity lactose from milk whey, comprise the following steps:
Step 1) fresh milk is carried out first time centrifugally operated, remove upper-layer fat, obtain skimmed milk;
Step 2) at a first pressure by skimmed milk by carrying out second time centrifugally operated after microfiltration membrane, extract supernatant liquor, obtain whey; After whey is carried out pasteurize, by the first ultra-filtration membrane under the second pressure, obtain the first penetrant after by described first ultra-filtration membrane;
Step 3) add acetic acid-sodium chloride solution by described first penetrant, until the pH value of the first permeate solution is carry out second time ultrafiltration, the second penetrant obtained after 5.0;
Step 4) the second penetrant is added dropwise in the sodium hydroxide solution of 0.1wt% ~ 5wt%, plate-and-frame filter press press filtration is passed through after waiting for 15min, filter screen obtains the first retentate, first retentate is dissolved in 0.08wt% ~ 1.6wt% hydrochloric acid soln, under the 3rd pressure, pass through nanofiltration membrane after waiting for 2h, obtain the 4th penetrant;
Step 5) in the 4th permeate solution, add lactose crystalline substance gradually, and the 4th permeate solution is heated, until boiled dry by solution, obtain lactose.
Preferably, described first time the rotating speed of centrifugally operated be 4000r/min ~ 5500r/min, centrifugal 15min.
Preferably, the rotating speed of described second time centrifugally operated is 5000r/min ~ 7000r/min, centrifugal 20min.
Preferably, described step 2) in, obtain described whey after the 12 ~ 24h that dialysed between 3 ~ 8 degrees Celsius by supernatant liquor.
Preferably, described first pressure is 0.03MPa ~ 0.05MPa.
Preferably, described second pressure is 0.15MPa ~ 0.20MPa.
Preferably, described 3rd pressure is 0.20MPa ~ 0.28MPa.
Preferably, described step 2) in, after whey being heated to 35 ~ 45 degrees Celsius, then by described first ultra-filtration membrane.
Preferably, described step 4) in, after the first retentate solution is heated to 38 ~ 45 degrees Celsius, then by described nanofiltration membrane.
Preferably, described step 2) in, by supernatant liquor by activated carbon column, then carry out pasteurize.
The present invention at least comprises following beneficial effect:
1, extracting method of the present invention, fully extract the lactose in whey, the extraction yield of lactose is high, reduces tooling cost, improves the purity of lactose simultaneously;
2, extracting method of the present invention can not introduce other foreign ions in the process extracting whey-protein, further increases the purity of lactose powder product.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet extracting high purity lactose from milk whey.
Embodiment
The present invention is described in further detail below, can implement according to this with reference to specification sheets word to make those skilled in the art.
Should be appreciated that used in the present inventionly such as " to have ", other element one or more do not allotted in " comprising " and " comprising " term or the existence of its combination or interpolation.
To achieve these goals, the present invention is by the following technical solutions: a kind of method extracting high purity lactose from milk whey, as shown in Figure 1, comprises the following steps:
Step 1) fresh milk is carried out first time centrifugally operated on whizzer, remove upper-layer fat, obtain skimmed milk, described first time, the rotating speed of centrifugally operated was 4000r/min ~ 5500r/min, and in the present embodiment, the rotating speed of whizzer adopts 4500r/min, after centrifugal 15min, after leaving standstill for some time, upper-layer fat, in the superiors, extracts by the fat in milk;
Step 2) at a first pressure by skimmed milk by after microfiltration membrane, the milk after degreasing is carried out second time centrifugally operated on whizzer, and the rotating speed of described second time centrifugally operated is 5000r/min ~ 7000r/min, centrifugal 20min.Then extract supernatant liquor, obtain whey; After whey is carried out pasteurize, by the first ultra-filtration membrane under the second pressure, carry out ultrafiltration, obtain the first penetrant after by described ultra-filtration membrane;
Step 3) add acetic acid-sodium chloride solution by described first penetrant, until the pH value of the first permeate solution is carry out second time ultrafiltration, the second penetrant obtained after 5.0;
Step 4) the second penetrant is added dropwise in the sodium hydroxide solution of 0.1wt% ~ 5wt%, plate-and-frame filter press press filtration is passed through after waiting for 15min, filter screen obtains the first retentate, first retentate is dissolved in 0.08wt% ~ 1.6wt% hydrochloric acid soln, under the 3rd pressure, pass through nanofiltration membrane after waiting for 2h, obtain the 4th penetrant;
Step 5) in the 4th permeate solution, add lactose crystalline substance gradually, and the 4th permeate solution is heated, until boiled dry by solution, obtain lactose crystal, pulverize, sieve filling.
In technique scheme, described step 2) in, obtain described whey after the 12 ~ 24h that dialysed between 3 ~ 8 degrees Celsius by supernatant liquor, in the present embodiment, dialyse supernatant liquor under the condition of 4 degrees Celsius 18h.
In technique scheme, described first pressure is 0.03MPa ~ 0.05MPa, and the present embodiment adopts 0.04MPa.
In technique scheme, described second pressure is 0.15MPa ~ 0.20MPa, and the present embodiment adopts 0.18MPa.
In technique scheme, described 3rd pressure is 0.20MPa ~ 0.28MPa, and the present embodiment adopts 0.26MPa.
In technique scheme, described step 2) in, after whey being heated to 35 ~ 45 degrees Celsius, then by described ultra-filtration membrane; In the present embodiment, whey is heated to 45 degrees Celsius, carrys out other compositions in maximum filtering whey with this, reach the accounting improving lactose.
In technique scheme, described step 2) in, after whey being heated to 35 ~ 45 degrees Celsius, then by described first ultra-filtration membrane.The present embodiment adopts 42 degrees Celsius.
In technique scheme, described step 4) in, after the first retentate solution is heated to 38 ~ 45 degrees Celsius, then by described nanofiltration membrane.In the present embodiment, adopt 45 degrees Celsius.
In technique scheme, described step 2) in, by supernatant liquor by activated carbon column, then carry out pasteurize, supernatant liquor is the transmittance in order to improve supernatant liquor by activated carbon column, with other compositions in filtering supernatant liquor.
From the above mentioned, extracting method of the present invention, fully extract the lactose in whey, the extraction yield of lactose is high, reduces tooling cost, improves the purity of lactose simultaneously; Meanwhile, extracting method of the present invention can not introduce other foreign ions in the process extracting whey-protein, further increases the purity of lactose powder product.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification sheets and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (10)
1. from milk whey, extract a method for high purity lactose, it is characterized in that, comprise the following steps:
Step 1) fresh milk is carried out first time centrifugally operated, remove upper-layer fat, obtain skimmed milk;
Step 2) at a first pressure by skimmed milk by carrying out second time centrifugally operated after microfiltration membrane, extract supernatant liquor, obtain whey; After whey is carried out pasteurize, by the first ultra-filtration membrane under the second pressure, obtain the first penetrant after by described first ultra-filtration membrane;
Step 3) add acetic acid-sodium chloride solution by described first penetrant, until the pH value of the first permeate solution is carry out second time ultrafiltration, the second penetrant obtained after 5.0;
Step 4) the second penetrant is added dropwise in the sodium hydroxide solution of 0.1wt% ~ 5wt%, plate-and-frame filter press press filtration is passed through after waiting for 15min, filter screen obtains the first retentate, first retentate is dissolved in 0.08wt% ~ 1.6wt% hydrochloric acid soln, under the 3rd pressure, pass through nanofiltration membrane after waiting for 2h, obtain the 4th penetrant;
Step 5) in the 4th permeate solution, add lactose crystalline substance gradually, and the 4th permeate solution is heated, until boiled dry by solution, obtain lactose.
2. the method extracting high purity lactose from milk whey according to claim 1, is characterized in that, described first time the rotating speed of centrifugally operated be 4000r/min ~ 5500r/min, centrifugal 15min.
3. the method extracting high purity lactose from milk whey according to claim 2, is characterized in that, the rotating speed of described second time centrifugally operated is 5000r/min ~ 7000r/min, centrifugal 20min.
4. the method extracting high purity lactose from milk whey according to claim 3, is characterized in that, described step 2) in, obtain described whey after the 12 ~ 24h that dialysed between 3 ~ 8 degrees Celsius by supernatant liquor.
5. from milk whey, extract the method for high purity lactose as claimed in claim 4, it is characterized in that, described first pressure is 0.03MPa ~ 0.05MPa.
6. from milk whey, extract the method for high purity lactose as claimed in claim 5, it is characterized in that, described second pressure is 0.15MPa ~ 0.20MPa.
7. from milk whey, extract the method for high purity lactose as claimed in claim 6, it is characterized in that, described 3rd pressure is 0.20MPa ~ 0.28MPa.
8. from milk whey, extract the method for high purity lactose as claimed in claim 7, it is characterized in that, described step 2) in, after whey being heated to 35 ~ 45 degrees Celsius, then by described first ultra-filtration membrane.
9. from milk whey, extract the method for high purity lactose as claimed in claim 8, it is characterized in that, described step 4) in, after the first retentate solution is heated to 38 ~ 45 degrees Celsius, then by described nanofiltration membrane.
10. from milk whey, extract the method for high purity lactose as claimed in claim 9, it is characterized in that, described step 2) in, by supernatant liquor by activated carbon column, then carry out pasteurize.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510941201.6A CN105541928A (en) | 2015-12-16 | 2015-12-16 | Method for extracting high purity lactose from milk whey |
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| CN201510941201.6A CN105541928A (en) | 2015-12-16 | 2015-12-16 | Method for extracting high purity lactose from milk whey |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108260800A (en) * | 2016-12-31 | 2018-07-10 | 烟台大学 | The technique for preparing Lactulose liquid using whey |
| CN108264528A (en) * | 2016-12-31 | 2018-07-10 | 烟台大学 | A kind of method that lactose is isolated and purified from whey |
| CN110680925A (en) * | 2018-07-04 | 2020-01-14 | 山东迪星药用辅料有限公司 | Medicinal lactose suitable for injection and preparation method thereof |
| CN111919908A (en) * | 2020-07-07 | 2020-11-13 | 石家庄君乐宝乳业有限公司 | Preparation method of desalted whey powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108260800A (en) * | 2016-12-31 | 2018-07-10 | 烟台大学 | The technique for preparing Lactulose liquid using whey |
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| CN110680925B (en) * | 2018-07-04 | 2022-10-11 | 山东迪星生物技术有限公司 | Medicinal lactose suitable for injection and preparation method thereof |
| CN111919908A (en) * | 2020-07-07 | 2020-11-13 | 石家庄君乐宝乳业有限公司 | Preparation method of desalted whey powder |
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