CN104473292A - Probiotic solid beverage for infants - Google Patents
Probiotic solid beverage for infants Download PDFInfo
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- CN104473292A CN104473292A CN201410801469.5A CN201410801469A CN104473292A CN 104473292 A CN104473292 A CN 104473292A CN 201410801469 A CN201410801469 A CN 201410801469A CN 104473292 A CN104473292 A CN 104473292A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses aprobiotic solid beverage for infants. The probioticsolid beverage comprises components in percentage by weight as follows: 15-25% of bifidobacterium powder, 15-25% of lactobacillus rhamnosus powder, 10-30% of rice protein powder, 10-20% of bovine colostrum powder, 5-15% of maltodextrin, 5-10% of fructo-oligosaccharide and 5-10% of galactooligosaccharide. The probioticsolid beverage is natural in component, scientific in formula, balanced in nutrition, convenient to drink, free of caking, good in dissolving ability and unique in taste and has effects of improving intestinal tracts of the infants, improving digestion and enhancing the immunity.
Description
Technical field
The present invention relates to containing additive, the dietetic product changing the nutritive quality of food, especially a kind of infant probio solid beverage.
Background technology
Infant belongs to the colony that digestive function is more weak, immunity is more weak, uses chemicals can bring injury to the baby of these sensitivities, and infant is again a pretty troublesome colony simultaneously, can not express various discomfort more exactly.Therefore, what adopt this uniqueness of probio seems very necessary with the natural ecological therapy of bacterium bacterium concerning baby.
Probio is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, can produce definite health efficacy thus improve host's microecological balance, plays the active beneficial microorganism general name of beneficial effect.The probio that scientist has found can be divided into three major types substantially, comprising: 1. lactobacillus class (as lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, Raman lactobacillus etc.); 2. Bifidobacterium class (as bifidobacterium longum, bifidobacterium breve, avette Bifidobacterium, bifidobacterium thermophilum etc.); 3. gram-positive cocci (as streptococcus fecalis, galactococcus, intermediary streptococcus etc.).In addition, some saccharomycete and enzyme is also had also can be included into the category of probio.
Nowadays, probio is added in infant food and prebiotics has become a kind of trend.Such as the applicant discloses a kind of stachyose probio solid beverage and preparation method thereof in first patent application 201310587465.7, and its component is stachyose 5 ~ 20%, Bifidobacteria powder 2 ~ 10%, lactobacillus acidophilus bacterium powder 2 ~ 10%, PURE WHEY 3 ~ 8%, milk powder 5 ~ 15%, grain swelling powder 10 ~ 40%, steviol glycoside 5 ~ 15%, maltodextrin 5 ~ 15%, citric acid 5 ~ 15%, flavoring essence 0.5 ~ 2%.But the colony that the present invention is directed to is the adult having certain enteron aisle digestion power, for infant, its security needs to be investigated, and is not suitable for the additive of infant containing some.Therefore, the probio solid beverage developing a kind of applicable infant taking is a new problem.
Summary of the invention
The object of this invention is to provide a kind of component natural, scientific formulation, brew is good, taking convenience, has and improves infant's enteron aisle, promotes the infant probio solid beverage of digestion and develop immunitypty function.
The technical solution adopted for the present invention to solve the technical problems is: a kind of infant probio solid beverage, it is characterized in that: it is made up of the component of following weight percents: Bifidobacteria powder 15 ~ 25%, Lactobacillus rhamnosus bacterium powder 15 ~ 25%, rice protein powder 10 ~ 30%, bovine colostrum 10 ~ 20%, maltodextrin 5 ~ 15%, FOS 5 ~ 10%, galactooligosaccharide 5 ~ 10%, the percentage by weight sum of each component is 100%.
Precedence technique scheme of the present invention is: the percentage by weight of described each component is: Bifidobacteria powder 20%, Lactobacillus rhamnosus bacterium powder 20%, rice protein powder 20%, bovine colostrum 14%, maltodextrin 10%, FOS 8%, galactooligosaccharide 8%.
Infant probio solid beverage of the present invention adds All Pure Nature raw material and probio, and it is balanced in nutrition.Wherein, the nutrition of the probio such as Lactobacillus rhamnosus, Bifidobacterium and human body digested and assimilated substantial connection, harmless and useful in enteron aisle, energy synthetic vitamin, assist digestion food, helps nutrient absorption, promote metabolism, reduce the generation of harmful substance and accelerate the eliminating of harmful substance; Rice protein is primarily of four kinds of albumen compositions such as albumin, globulin, protein,alcohol-soluble and glutelin.In corn gluten protein, the biological value of rice protein is very high, its nutritive value can compare favourably with egg, cow's milk, beef, the amino acid Compositional balance of rice protein is reasonable, and amino acid content is high, in addition, rice protein is opposing original shape albumen, can not produce allergic reaction, be very favourable to production infant food; Cow initial milk protein matter content is higher, and fat and sugar content is lower, not only containing abundant nutriment, and containing a large amount of immune factors and growth factor, as immunoglobulin (Ig), lactoferrin, lysozyme, insulin-like growth factor, EGF etc., there is immunological regulation, improve intestines and stomach, enhancing development, improve senile symptom, suppress the physiological active functionses such as multiple germ; Maltodextrin also claims water-soluble dextrin or enzyme process dextrin, is to make raw material with each kind of starch, forms through the conversion of enzyme method technique low degree controlled hydrolysis, purification, drying.Volumetric expansion when it is taken after mixing it with water, prevented from caking, instant, brew is good, is easily absorbed by the body, and improves the nutrition rate of exchange.In infant probio solid beverage of the present invention, with the addition of the probio of applicable infant, and in conjunction with the rice protein of resistance high-quality and bovine colostrum, contribute to infant's enteron aisle and immune growth especially, nurture baby prevention of disease can be guaranteed.First, probio produces protease in enteron aisle, and hydrolyzable produces more free amino acid and polypeptide, improves biological value.Secondly, FOS and galactooligosaccharide can not be decomposed by various digestive ferment in human body alimentary canal.FOS in enteron aisle can by the beneficial bacterium such as lactobacillus acidophilus, Bifidobacterium Selection utilization, make them fast and amount reproduction, exist in enteron aisle and can reduce pH value, increase the absorption of mineral matter, the generation of toxic metabolite and harmful enzyme in enteron aisle can be reduced simultaneously.Each component synergy, can improve infant's enteron aisle, causes enteron aisle acidity to reduce and suppresses spoilage organisms, promote that it digests by organic acid in decomposition enteron aisle; Supplement required vitamin, probio produces various vitamin, suppresses the decomposition of some to ensure keeping simultaneously; Strengthen immunologic function, producing intestine immunity cell stimulates, and improves immunity.
Infant probio solid beverage component of the present invention is natural, scientific formulation, and balanced in nutrition, taking convenience, does not lump, and brew is good, and mouthfeel is unique, has and improves infant's enteron aisle, promotes the function of digestion and develop immunitypty.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Raw materials usedly in the present embodiment be commercial products, wherein,
Bifidobacteria powder is purchased from promise Shen, Shanghai, and its content is>=2.5 × 10
7; Lactobacillus rhamnosus bacterium powder is purchased from promise Shen, Shanghai, and its content is>=5.0 × 10
7; The nutrition of the probio such as Lactobacillus rhamnosus, Bifidobacterium and human body digested and assimilated substantial connection, normal flora has participated in absorption and the metabolism such as cholesterol, steroids, fat, protein, amino acid.These floras are harmless and useful in enteron aisle, energy synthetic vitamin, and assist digestion food, helps nutrient absorption, promotes metabolism, overcomes decay process, reduce the generation of harmful substance and accelerate the eliminating of harmful substance.
Rice protein powder is purchased from the Zhengzhou feudal dynasty, and its protein content is >=80%; Its effect: this rice protein is primarily of four kinds of albumen compositions such as albumin, globulin, protein,alcohol-soluble and glutelin.In corn gluten protein, the biological value of rice protein is very high, and its nutritive value can compare favourably with egg, cow's milk, beef; The amino acid Compositional balance of rice protein is reasonable, and amino acid content is high, be other vegetable protein incomparable, be therefore acknowledged as the food protein of high-quality.In addition, rice protein is opposing original shape albumen, can not produce allergic reaction, is very favourable to production infant food.
Bovine colostrum is purchased from Heilungkiang Kang Pu, and its immunoglobulin content is >=20%; Its effect: cow initial milk protein matter content is higher, and fat and sugar content is lower, not only containing abundant nutriment, and containing a large amount of immune factors and growth factor, as immunoglobulin (Ig), lactoferrin, lysozyme, insulin-like growth factor, EGF etc., prove through scientific experiment, there is immunological regulation, improve intestines and stomach, enhancing development, improve senile symptom, suppress the physiological active functionses such as multiple germ.
Maltodextrin is purchased from Xi Wang group; This maltodextrin also claims water-soluble dextrin or enzyme process dextrin, is to make raw material with each kind of starch, forms through the conversion of enzyme method technique low degree controlled hydrolysis, purification, drying.Volumetric expansion when it is taken after mixing it with water, prevented from caking, instant, brew is good, is easily absorbed by the body, and improves the nutrition rate of exchange.
FOS, galactooligosaccharide are precious purchased from bowling.
In infant probio solid beverage of the present invention, with the addition of the probio of applicable infant, and in conjunction with the rice protein of resistance high-quality and bovine colostrum, contribute to infant's enteron aisle and immune growth especially, nurture baby prevention of disease can be guaranteed.Meanwhile, probio produces protease in enteron aisle, and hydrolyzable produces more free amino acid and polypeptide, improves biological value.Secondly, FOS and galactooligosaccharide can not be decomposed by various digestive ferment in human body alimentary canal, and the FOS in enteron aisle can, by the beneficial bacterium such as lactobacillus acidophilus, Bifidobacterium Selection utilization, make them fast and amount reproduction.Each component synergy, can improve infant's enteron aisle, causes enteron aisle acidity to reduce and suppresses spoilage organisms, promote that it digests by organic acid in decomposition enteron aisle; Supplement required vitamin, probio produces various vitamin, suppresses the decomposition of some to ensure keeping simultaneously; Strengthen immunologic function, producing intestine immunity cell stimulates, and improves immunity.
embodiment 1
A kind of infant probio solid beverage, it is made up of the component of following weight percents: Bifidobacteria powder 20%, Lactobacillus rhamnosus bacterium powder 20%, rice protein powder 20%, bovine colostrum 14%, maltodextrin, 10%, FOS 8%, galactooligosaccharide 8%.The infant probio solid beverage that the present embodiment provides, causes enteron aisle acidity to reduce by organic acid in decomposition enteron aisle and suppresses spoilage organisms, promoting that it digests; Supplement needed by human body vitamin, probio produces various vitamin, suppresses the decomposition of some to ensure keeping simultaneously; Strengthen immunologic function, producing intestine immunity cell stimulates, and improve immunity, have component natural, scientific formulation, balanced in nutrition, taking convenience, does not lump, and brew is good, the advantage of mouthfeel uniqueness.
embodiment 2
A kind of infant probio solid beverage, it is made up of the component of following weight percents: Bifidobacteria powder 15%, Lactobacillus rhamnosus bacterium powder 25%, rice protein powder 10%, bovine colostrum 20%, maltodextrin 15%, FOS 5%, galactooligosaccharide 10%.The infant probio solid beverage component that the present embodiment provides is natural, scientific formulation, and balanced in nutrition, taking convenience, does not lump, and brew is good, and mouthfeel is unique, has and improves infant's enteron aisle, promotes the function of digestion and develop immunitypty.
embodiment 3
A kind of infant probio solid beverage, it is made up of the component of following weight percents: Bifidobacteria powder 25%, Lactobacillus rhamnosus bacterium powder 15%, rice protein powder 30%, bovine colostrum 10%, maltodextrin 5%, FOS 10%, galactooligosaccharide 5%.The infant probio solid beverage that the present embodiment provides causes enteron aisle acidity to reduce by organic acid in decomposition enteron aisle and suppresses spoilage organisms, improves enteron aisle, promotes that it digests; Probio produces various vitamin, suppresses some decomposition to ensure keeping simultaneously; Probio produces intestine immunity cell to be stimulated, and improve immunity, have component natural, scientific formulation, balanced in nutrition, taking convenience, does not lump, and brew is good, the advantage of mouthfeel uniqueness.
embodiment 4
A kind of infant probio solid beverage, it is made up of the component of following weight percents: Bifidobacteria powder 22%, Lactobacillus rhamnosus bacterium powder 18%, rice protein powder 15%, bovine colostrum 15%, maltodextrin 12%, FOS 9%, galactooligosaccharide 9%.The infant probio solid beverage component that the present embodiment provides is natural, scientific formulation, and balanced in nutrition, taking convenience, does not lump, and brew is good, and mouthfeel is unique, has and improves infant's enteron aisle, promotes the function of digestion and develop immunitypty.
product involved by the embodiment of the present invention 1 ~ 4 is prepared from by following process steps:
(1) prepare burden: Bifidobacteria powder, bacillus acidophilus bacterium powder, rice protein powder, bovine colostrum, maltodextrin, FOS, galactooligosaccharide raw material are proceeded in 300,000 grades of clean areas, sampling is tested;
(2) sieve: after the assay was approved, cross 80 mesh sieves respectively, take raw material according to the proportioning given by each embodiment;
(3) mix: stirred on the mixing limit, raw material limit taken, be mixed into color and luster consistent, no color differnece, obtains total mixed powder;
(4) pack: packed by preset weight by mixed powder, labeling, vanning, obtains finished product.
carry out animal experiment with infant probio solid beverage given by embodiment 1 ~ 4 below, to further illustrate the present invention, there is the effect promoting gastrointestinal absorption, develop immunitypty.
1) materials and methods
1.1) sample: according to the embodiment of the present invention 1 ~ 4 provide preparation method to prepare the solid beverage of 1g/ bag.Human oral's recommended amounts is every day 1 time, each one bag.
1.2) animal used as test and grouping: body weight is the SPF level Kunming kind female mice 50 of 18g-22g, is provided, production licence number SCXK (Henan) 2005-0001 by Henan Province's Experimental Animal Center.Feed is provided by Changsha Kaifu District Dong Chuan Animal Science service department, and production licence number is SCXK (Hunan) 2006-0001.Be divided into control group, embodiment 1-4 group at random.Control group is with isodose starch feeding.
1.3) experimental situation condition: experiment condition is barrier environment, temperature 22 DEG C-24 DEG C, humidity 52%-54%.
1.4) experimental technique:
1.4.1) the mouse lymphocyte transformation experiment (mtt assay) of ConA induction: asepticly get spleen, make cell suspension, Hank ' s liquid washes 2 times, adjustment cell concentration is 3 × 106/ml.Join in 24 well culture plates by a cell suspension point holes, every hole 1ml, a hole adds 75ulConA liquid (7.5ug/ml), and another hole is contrast, puts 5% carbon dioxide, cultivates 72h for 37 DEG C.Cultivation terminates front 4h, and every hole sucks supernatant 0.7ml, adds 0.7ml not containing the RPMI1640 nutrient solution of calf serum, adds MTT (5mg/ml) 50ul/ hole simultaneously, continues to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve completely, measures OD value with 570nm wavelength.
1.4.2) antibody-producting cell detect (Jerne improves slide method): get sheep blood brine 3 times, hematocrit SRBC physiological saline be made into 2%(v/v) cell suspension, every mouse lumbar injection 0.2ml.Immunity 5 days after, put to death mouse, get spleen, 200 eye mesh screens filter, washing, centrifugal 2 times, finally cell is suspended in 8mlHanks liquid.Adjustment cell concentration is 5 × 106/ml.By top layer culture medium heating for dissolving, mix with equivalent 2 times of concentration Hanks liquid, packing small test tube, often pipe 0.5ml, add 50ul10%SRBC, 20ul splenocyte suspension, mixing, reviewing, incubation 1.5h in CO2gas incubator, add the complement (1: 8) with the dilution of SA liquid, continue incubation 1.5h, counting hemolysis plaque number.
1.4.3) food utilization of duration of test record every animal.
2) result
2.1) sample is on the impact of mouse lymphocyte conversion capability
Table 1 sample is on the impact of mouse lymphocyte conversion capability
| Group | Number of animals | Lymphopoiesis ability (OD difference) | |
| (only) | x±s | P value | |
| Control group | 10 | 0.017±0.012 | - |
| Embodiment 1 | 10 | 0.038±0.013 | 0.023 |
| Embodiment 2 | 10 | 0.033±0.021 | 0.032 |
| Embodiment 3 | 10 | 0.026±0.022 | 0.044 |
| Embodiment 4 | 10 | 0.017±0.025 | 0.036 |
From table 1, per os gives the sample 30 days of different embodiment, and embodiment 1 can increase mouse lymphocyte conversion capability, has conspicuousness (P<0.05) with control group comparing difference.
2.2) sample is on the impact of humoral immunity
Table 2 sample is on the impact of mouse antibodies cellulation number
| Group | Number of animals | Hemolysis plaque number | |
| (only) | (individual/10 6Individual splenocyte) x ± s | P value | |
| Control group | 10 | 111±43 | - |
| Embodiment 1 | 10 | 163±44 | 0.015 |
| Embodiment 2 | 10 | 141±43 | 0.048 |
| Embodiment 3 | 10 | 153±32 | 0.019 |
| Embodiment 4 | 10 | 149±34 | 0.017 |
As shown in Table 2, per os gives the sample 30 days of the different embodiment of mouse, and embodiment 1 can increase mouse antibodies cellulation number, has conspicuousness (P<0.05) with control group comparing difference.
2.3) sample is on the impact of mouse food-intake and food utilization
Table 3 sample is on the impact of mouse food-intake and food utilization
As can be seen from experimental data, in laboratory conditions, per os gives the sample 30 days that mouse embodiment 1 ~ 4 produces, as can be seen from Table 3, each embodiment animal feed combination food utilization control group is compared, and has significant difference (P < 0.05).Embodiment 1 can increase Mice Mice lymphocyte transformation ability, antibody-producting cell number, has conspicuousness (P<0.05) with control group comparing difference, illustrates that the present invention has the function of develop immunitypty, and with embodiment 1 for preferred plan.
Claims (2)
1. an infant probio solid beverage, it is characterized in that: it is made up of the component of following weight percents: Bifidobacteria powder 15 ~ 25%, Lactobacillus rhamnosus bacterium powder 15 ~ 25%, rice protein powder 10 ~ 30%, bovine colostrum 10 ~ 20%, maltodextrin 5 ~ 15%, FOS 5 ~ 10%, galactooligosaccharide 5 ~ 10%, the percentage by weight sum of each component is 100%.
2. a kind of infant probio solid beverage according to claim, is characterized in that: the percentage by weight of described each component is respectively: Bifidobacteria powder 20%, Lactobacillus rhamnosus bacterium powder 20%, rice protein powder 20%, bovine colostrum 14%, maltodextrin 10%, FOS 8%, galactooligosaccharide 8%.
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