CN108260473A - Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma - Google Patents

Abstract

The invention discloses the methods using eucalyptus industrial wood waste as main culture material cultivating ganoderma, include the following steps：(1) pre-treatment；(2) it stocks up：Prepare following raw material：Eucalyptus bits, eucalyptus barks, cotton seed hulls, wheat bran, calcium superphosphate, gypsum and fermentation at elevated temperatures agent；(3) spice and fermentation；(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content and pH value of above-mentioned material, then polyethylene cylinder material bag or polypropylene cylinder material bag is selected to carry out pack processing, obtains bacterium bag；(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, using normal-pressure sterilization；When carrying out packed using polypropylene cylinder material bag, using high pressure sterilization；(6) it is inoculated with；(5) bacterium germination and management of producing mushroom.The present invention provides theoretical foundation to filter out eucalyptus industrial wood waste as the ganoderma lucidum high-yield and high-efficiency localization raw material cultivation formula of major ingredient and cultivate success for Aqueous Extract From Guangxi Ganoderma Lucidum production.

Description

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma

【Technical field】

The present invention relates to the cultivation field of ganoderma lucidum, more particularly to using eucalyptus industrial wood waste as main culture material cultivating ganoderma Method.

【Background technology】

Ganoderma lucidum is the general name of Basidiomycetes Polyporaceae Ganoderma red sesame and purple sesame, is a kind of precious medicine-food two-purpose fungi. Ganoderma lucidum contains a variety of active ingredients such as polysaccharide, triterpenes, peptides, nucleosides, can improve the immunity of the human body, and inhibits tumour cell life It is long, it may have the effects that anti-aging is anti-oxidant, liver protecting.In recent years, with the development of the social economy, people consume ganoderma lucidum Demand is increasing, and the deficiency of Wild ganoderma resource, makes the scale of cultivating ganoderma increase year by year.Traditional cultivation of glossy ganoderma is with linden For primary raw material, far from meeting the market demand, adaptation to local conditions develop substituting stuff cultivation by be cultivation of glossy ganoderma industry main hair Open up direction.Eucalyptus bits are eucalyptus processing byproducts, and Guangxi wood-processing industry generates a large amount of Eucalyptus bits every year, wherein most of press Tradition stacks discard processing, and exudate can often cause seriously to pollute to soil and water body.Eucalyptus considers recycling to be worth doing, can both disappear Except its pollution to environment, the level of aggregation of Eucalyptus Wood processing industry can be also improved.It is enriched using Guangxi locality and cheap eucalyptus Sawdust has positive impetus, while can also improve the exploitation value of Eucalyptus bits as cultivation of glossy ganoderma raw material to cultivation of glossy ganoderma Value, eliminates its pollution to environment.

Ganoderma lucidum substituting stuff cultivation, raw material are usually timber processing byproduct, tree trimming leftover bits and pieces or agricultural and sideline product.Lv Mingliang Deng (matrix cultivates log glossy ganoderma influence [J] edible fungi of china of appearance and yield, 2008,27 (5)：22~24.) with difference Seeds are raw material cultivating ganoderma, find ganoderma lucidum when different tree species are cultivated, fructification appearance luster etc. has notable difference, illustrates original Material to ganoderma lucidum growth has a major impact, selection cultivation of glossy ganoderma raw material need to carry out corresponding cultivation experiments, understand its if appropriate for It uses.Liu Mingxiang etc. (screening [J] University Of Agriculture and Forestry In Fujian's journals (natural science edition) of tea twig powder substituting stuff cultivation ganoderma strain, 2012,41 (2)：183~186.) using tea twig powder as raw material, the experiment of ganoderma lucidum substituting stuff cultivation has been carried out, the results showed that, it is cultivated Mycelial growth rate of the bacterial strain when tea twig powder is cultivated goes out the difference that sesame rate, biological transformation ratio are cultivated than weed tree sawdust.Chen Jimin Deng (mango cultivation on sawdust ganoderma lucidum experiment [J] China melon dish, 2013,26 (3)：43~45.) trade trimmed using Zhuhai locality Mango cultivating ganoderma finds that mango sawdust is suitable as the raw material of ganoderma lucidum substituting stuff cultivation.But due to the annual only ability before typhoon Trimming, and the branch cut is limited, has certain limitation for extensive application.At present using eucalyptus sawdust as raw material cultivating ganoderma Study fewer, (eucalyptus cultivation on sawdust edible mushroom [J] edible mushroom journals, 2011,18 (3) such as summer phoenix Na：42~44.) with skin The eucalyptus sawdust that bits, bar bits, skin bar mixing bits are composed is cultivation matrix, to ganoderma lucidum, elegant precious mushroom, pleurotus eryngii and needle mushroom Preliminary experiment in cultivation has been carried out Deng 4 kinds of edible mushrooms, the results showed that, ganoderma lucidum mixes bits (skin: bar=1: 3) compost in eucalyptus barks bar In can with normal growth, but not to culturing raw material proportioning optimize.Zhong Liyi (Eucalyptus Planting purple sesame and Analysis of Nutritive Composition [J] edible fungi of china, 2014,33 (3) 24~26.) with eucalyptus cultivation basswood purple sesame, the results showed that mycelia grows on Eucalyptus It is faster than control group, and when annual output is then with compareing close, difference unobvious.

Ganoderma lucidum substituting stuff cultivation is based on cotton seed hulls and weed tree sawdust at present, and the non-cotton production areas in Guangxi, cotton seed hulls price are them Several times of its local cost of material differ, and too high cost of material is unfavorable for improving the competitiveness of Aqueous Extract From Guangxi Ganoderma Lucidum cultivation industry.It is miscellaneous Wood raw material ingredient is not fixed, and is caused ganoderma lucidum production and output unstable, is unfavorable for that management is factory produced, it is difficult to ensure each batch The product features of secondary ganoderma lucidum bring uncertainty to the production and sales of manufacturing enterprise.Guangxi is eucalyptus main producing region, annual to generate greatly Measure cheap Eucalyptus bits, it is ensured that cultivation of glossy ganoderma raw material stablizes supply.Therefore, it studies a kind of stable quality, processed with eucalyptus Residue is the method for main culture material cultivating ganoderma, has vast researching value.

【Invention content】

The object of the present invention is to provide the methods using eucalyptus industrial wood waste as main culture material cultivating ganoderma, and first Eucalyptus is considered to be worth doing It is pre-processed with by bark, then passes through spice and fermentation, pack, sterilizing, inoculation and bacterium germination and management of producing mushroom, cultivation spirit Sesame.The cultivation formula of ganoderma lucidum can not only be filtered out, moreover it is possible to explore the side using eucalyptus industrial wood waste as main culture material cultivating ganoderma Method, high-yield high-efficiency cultivation ganoderma lucidum, the production for Aqueous Extract From Guangxi Ganoderma Lucidum provide theoretical foundation.

In order to achieve the above objectives, the technical solution adopted in the present invention is：It is planted by main culture material of eucalyptus industrial wood waste The method for training ganoderma lucidum, includes the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 5~ 12cm long sections, are dried, and obtain eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：45~55 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 15~20 parts Cotton seed hulls, 10~15 parts of wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.1~0.3 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 60%~65%, while the pH value for adjusting above-mentioned material with lime mixes up moisture content and soda acid to 8.0~9.0 After degree by material pile height be 1.2m~1.5m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, in windrow Windrow is made a call to an air hole from the top down with the waddy of a diameter of 5cm~10cm, was carried out every 4 days primary by centre every 0.8m Turning, fermentation time are 8d~10d, obtain fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again be adjusted to 60%~65%, pH value and be adjusted to 7.0~8.0, then select thickness be 0.025mm, length × width be 230mm × 450mm, the polyethylene cylinder material bag of two open ends or thickness are 0.04mm, length × width is 170mm × 330mm, single open end it is poly- Propylene cylinder material bag carries out pack processing, obtains bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, carry out normal-pressure sterilization： Temperature is heated to 98 DEG C~100 DEG C in 6h under normal pressure, and is ceased fire after constant temperature holding 12h~16h, kettle temperature drop subject to sterilization It takes the dish out of the pot during to 60 DEG C~65 DEG C；

When carrying out packed using polypropylene cylinder material bag, high pressure sterilization is carried out：Pressure for 0.14MPa~0.15MPa and Temperature is steam sterilizing 2h~2.5h under conditions of 120 DEG C~123 DEG C, can be taken the dish out of the pot when pressure is down to 0；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 12~20g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized after inoculation, then It is fixed with sterilized two layers of newspaper, bacterium bag is moved in culturing room after the completion of inoculation and is cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

The invention has the advantages that：

The present invention is considered to be worth doing by the use of Eucalyptus, presses the Eucalyptus such as bark industrial wood waste as the Cultivation use of ganoderma lucidum, is equipped with suitable The materials such as cotton seed hulls, wheat bran, calcium superphosphate and gypsum not only greatly reduce the production cost of ganoderma lucidum, improve benefit, and are ganoderma lucidum Guarantee that production standardizes, industrialization furnishes ample material.At the same time it can also consider eucalyptus to be worth doing and turn waste into wealth by bark, reduce Environmental pollution extends the industrial chain of eucalyptus comprehensive utilization of resources, so as to form the benign cycle of eucalyptus industry and ganoderma lucidum industry hair Exhibition pattern promotes eucalyptus industry and the common health of ganoderma lucidum industry, quick, sustainable development.

【Specific embodiment】

Embodiment 1：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, include the following steps：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 5cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：45 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 15 parts of cotton seed hulls, 10 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.1 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 60%, while the pH value of above-mentioned material is adjusted to 8.0 with lime, mix up material after moisture content and acid-base value Pile height be 1.2m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 5cm from the top down, carries out a turning every 4 days, fermentation time 8d is obtained Fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 60%, pH value and is adjusted to 7.0, then selection thickness is 0.025mm, length × width is 230mm × 450mm, two open ends Polyethylene cylinder material bag carry out pack processing, obtain bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, carry out normal-pressure sterilization： Temperature is heated to 98 DEG C in 6h under normal pressure, and is ceased fire after constant temperature holding 12h, kettle temperature subject to sterilization goes out when being down to 60 DEG C Pot；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 20g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Embodiment 2：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 6cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：50 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 18 parts of cotton seed hulls, 11 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.15 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 62%, while the pH value of above-mentioned material is adjusted to 8.3 with lime, mix up material after moisture content and acid-base value Pile height be 1.4m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 6cm from the top down, carries out a turning every 4 days, fermentation time 9d is obtained Fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 64%, pH value and is adjusted to 7.5, then selection thickness is 0.025mm, length × width is 230mm × 450mm, two open ends Polyethylene cylinder material bag carry out pack processing, obtain bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, carry out normal-pressure sterilization： Temperature is heated to 99 DEG C in 6h under normal pressure, and is ceased fire after constant temperature holding 14h, kettle temperature subject to sterilization goes out when being down to 62 DEG C Pot；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 18g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Embodiment 3：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 8cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：48 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 16 parts of cotton seed hulls, 12 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.2 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 64%, while the pH value of above-mentioned material is adjusted to 8.4 with lime, mix up material after moisture content and acid-base value Pile height be 1.4m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 8cm from the top down, carries out a turning every 4 days, fermentation time 8.5d is obtained To fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 61%, pH value and is adjusted to 7.6, then selection thickness is 0.025mm, length × width is 230mm × 450mm, two open ends Polyethylene cylinder material bag carry out pack processing, obtain bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, carry out normal-pressure sterilization： Temperature is heated to 100 DEG C in 6h under normal pressure, and is ceased fire after constant temperature holding 16h, kettle temperature subject to sterilization goes out when being down to 65 DEG C Pot；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 17g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Embodiment 4：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 10cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：49 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 18 parts of cotton seed hulls, 13 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.2 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 64%, while the pH value of above-mentioned material is adjusted to 8.6 with lime, mix up material after moisture content and acid-base value Pile height be 1.3m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 7cm from the top down, carries out a turning every 4 days, fermentation time 8.5d is obtained To fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 62%, pH value and is adjusted to 7.8, then selection thickness is 0.04mm, length × width is 170mm × 330mm, single open end Polypropylene cylinder material bag carries out pack processing, obtains bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polypropylene cylinder material bag, carry out high pressure sterilization： The steam sterilizing 2h under conditions of pressure is 0.14MPa and temperature is 120 DEG C, can take the dish out of the pot when pressure is down to 0；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 14g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Experiment 5：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 8cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：51 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 18 parts of cotton seed hulls, 13 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.25 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 63%, while the pH value of above-mentioned material is adjusted to 8.5 with lime, mix up material after moisture content and acid-base value Pile height be 1.4m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 7cm from the top down, carries out a turning every 4 days, fermentation time 9d is obtained Fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 61%, pH value and is adjusted to 7.3, then selection thickness is 0.04mm, length × width is 170mm × 330mm, single open end Polypropylene cylinder material bag carries out pack processing, obtains bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polypropylene cylinder material bag, carry out high pressure sterilization： The steam sterilizing 2.2h under conditions of pressure is 0.145MPa and temperature is 121 DEG C, can take the dish out of the pot when pressure is down to 0；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 13g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Experiment 6：

Using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 12cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：55 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 20 parts of cotton seed hulls, 15 Part wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.3 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, add water tune It is wet, make water content up to 65%, while the pH value of above-mentioned material is adjusted to 9.0 with lime, mix up material after moisture content and acid-base value Pile height be 1.5m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, every 0.8m among windrow, use Windrow is made a call to an air hole by the waddy of a diameter of 10cm from the top down, carries out a turning every 4 days, fermentation time 10d is obtained To fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material Again it is adjusted to 65%, pH value and is adjusted to 8.0, then selection thickness is 0.04mm, length × width is 170mm × 330mm, single open end Polypropylene cylinder material bag carries out pack processing, obtains bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polypropylene cylinder material bag, carry out high pressure sterilization： The steam sterilizing 2.5h under conditions of pressure is 0.15MPa and temperature is 123 DEG C, can take the dish out of the pot when pressure is down to 0；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is carried out by sterile working regulation Inoculation, the sowing quantity of each bacterium bag is 12g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized, then with after inoculation Two layers of newspaper of sterilizing are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.

Experiment 1：

Cultivation of glossy ganoderma is tested：According to the method cultivating ganoderma of embodiment 1, the investigation and comparison different fermentations time is to cultivation of glossy ganoderma Influence.Separately designing this 5 times of 0d, 4d, 8d, 12d and 16d is handled, comparative analysis respectively processing to windrow temperature The influence of situation of change, pollution rate, bacterium germination situation, fruiting time and biological efficiency etc..Wherein at the different fermentations time The performance of reason cultivation of glossy ganoderma fruiting is shown in Table 1, and the performance of different fermentations time-triggered protocol Mycelium Growth of Ganoderma lucidum speed is shown in Table 2, different fermentations The performance of time-triggered protocol cultivation of glossy ganoderma economical character is shown in Table 3, and different disposal windrow thermograph is shown in Table 4.

Strains tested is the big ganoderma lucidum in the U.S., by the Breeding of Edible Mushroom research of Institute of Micro-biology of Guangxi Autonomous Region Academy of Agricultural Sciences Room, which preserves, to be provided.

Table 1：

Fermentation time (d)	Inoculation time	Pollution rate (%)	The purseful time	Fruiting time	Biological efficiency (%)
						0	2017.10.4	82	2017.10.23	2017.11.14	17.59
4	2017.10.4	96	2017.10.15	2017.10.25	31.07
						8	2017.10.4	94	2017.10.19	2017.10.27	30.11
12	2017.10.4	86	2017.10.25	2017.11.14	26.85
						16	2017.10.4	74	2017.10.29	2017.11.14	20.74

Table 2：

Fermentation time (d)	Average growth rate (mm/d)	The 1% pole level of signifiance	5% level of signifiance
				0	5.15	B	c
4	5.99	A	ab
				8	5.78	A	ab
12	5.53	AB	b
				16d	5.07	B	c

Table 3:

Table 4：

Consolidated statement 1-4, it is known that the result shows that, 5 processing, which are fermented from windrow to windrow temperature at the 4th day, is raised to peak Windrow temperature is begun to decline after 50~58 DEG C, the 5th day, by the eight to nine day after windrow temperature no longer rise, and begin to decline to 42 DEG C~45 DEG C；With the mycelium length of ferment 4d, 8d is most fast, growing way is best, pollution rate is minimum, and fruiting is neat, bioconversion Rate highest；Each character general performances of 0d and 16d of fermenting are all worst.

Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to Cover the scope of the claims in the present invention.

Claims (1)

1. using eucalyptus industrial wood waste as the method for main culture material cultivating ganoderma, it is characterised in that：Include the following steps：

(1) pre-treatment：It considered Eucalyptus to be worth doing 5 mesh to weed out except impurity, dry spare；The processing of fresh eucalyptus barks is ground into 5~12cm Long section, is dried, and obtains eucalyptus barks, spare；

(2) it stocks up：It counts in parts by weight, prepares following raw material：45~55 parts of Eucalyptus bits, 20 parts of eucalyptus barks, 15~20 parts of cottonseeds Shell, 10~15 parts of wheat bran, 1 part of calcium superphosphate, 1 part of gypsum and 0.1~0.3 part of fermentation at elevated temperatures agent；

(3) spice and fermentation：It is by the formula of step (2) that all raw material dry mixing in addition to wheat bran are uniform, damping is added water, is made Water content adjusts the pH value of above-mentioned material to 8.0~9.0 up to 60%~65%, while with lime, after mixing up moisture content and acid-base value By material pile height be 1.2m~1.5m, the material heap that width is 1m, material heap length is depending on the quantity of windrow, among windrow Every 0.8m, windrow is made a call into an air hole from the top down with the waddy of a diameter of 5cm~10cm, a turning was carried out every 4 days, Fermentation time is 8d~10d, obtains fermentation materials；

(4) it packs：Wheat bran is added in fermentation materials in proportion, and is stirred evenly, then by the water content of above-mentioned material again It is adjusted to 60%~65%, pH value and is adjusted to 7.0~8.0, then selection thickness is 0.025mm, length × width is 230mm × 450mm, two The polyethylene cylinder material bag or thickness is 0.04mm, length × width is 170mm × 330mm, the polypropylene cylinder material of single open end that head is open Bag carries out pack processing, obtains bacterium bag；

(5) it sterilizes：Bacterium bag and fashionable dress pot are sterilized, when carrying out packed using polyethylene cylinder material bag, carry out normal-pressure sterilization：Normal pressure Under temperature is heated to 98 DEG C~100 DEG C in 6h, and constant temperature keep 12h~16h after cease fire, kettle temperature subject to sterilization is down to 60 DEG C~65 DEG C when take the dish out of the pot；

When carrying out packed using polypropylene cylinder material bag, high pressure sterilization is carried out：It is 0.14MPa~0.15MPa and temperature in pressure Steam sterilizing 2h~2.5h under conditions of being 120 DEG C~123 DEG C, can take the dish out of the pot when pressure is down to 0；

(6) it is inoculated with：Bacterium bag after sterilizing is moved into transfer room, when material temperature is down to 35 DEG C, is inoculated with by sterile working regulation, The sowing quantity of each bacterium bag is 12~20g, and the inoculation mouth of bacterium bag first puts on the Plastibell sterilized after inoculation, then with having gone out Two layers of newspaper of bacterium are fixed, and are moved in culturing room bacterium bag after the completion of inoculation and are cultivated；

(5) bacterium germination and management of producing mushroom：Conventional bacterium germination, management of producing mushroom are carried out according to strain properties.