CN108245545A - A kind of Saussurea involucrata extract and its preparation method and application, the skin preparations for extenal use containing it - Google Patents
A kind of Saussurea involucrata extract and its preparation method and application, the skin preparations for extenal use containing it Download PDFInfo
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- CN108245545A CN108245545A CN201611243198.1A CN201611243198A CN108245545A CN 108245545 A CN108245545 A CN 108245545A CN 201611243198 A CN201611243198 A CN 201611243198A CN 108245545 A CN108245545 A CN 108245545A
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- saussurea involucrata
- extract
- water
- involucrata extract
- glacial water
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/285—Aucklandia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention discloses a kind of Saussurea involucrata extract and its preparation method and application, containing its skin preparations for extenal use.The Saussurea involucrata extract has the function of preferably to remove free radical and promotes human fibroblasts proliferation, has more preferably anti-aging effects, available for cosmetics, health food and field of medicaments, especially can be applied in cosmetics.
Description
Technical field
Present invention relates particularly to a kind of Saussurea involucrata extract and preparation method and application, include its skin preparations for extenal use.
Background technology
Harman in 1956 proposes free radical causes of senescence [Harman, D. Aging: A theory based on
free radical and radiation chemistry. J. Gerontol. 1956, 11:289-300.], it is believed that mistake
More free radicals is the major reason for causing biological aging.According to this theory, in vivo excessive active oxygen radical and insatiable hunger
It is acted on aliphatic acid and generates the substances such as malonaldehyde, malonaldehyde will produce brown pigment with the effects that protein on cell membrane, precipitate
It is become in skin as various color spots.Excessive free radical can also make collagenous fibres, elastomer in skin crosslink and become
Property becomes fragile, follows the string, and when moisture of skin deficiency, easily makes elastic fibers break, the skins such as dark line, microgroove, wrinkle occurs
Aging phenomenon.In addition, the environmental contaminants of the ionizing radiation and air pollution and chemical substance etc. in environment, can also make
It is constantly generated free radicals in organism.Such as the ultraviolet light in environment can make the fibroblast and mitochondria in dermis of skin
Be stimulated, and then release superoxide anion, and excessive superoxide anion can then be converted into other destructiveness it is stronger oneself
By base.Although there is the Antioxidative Defense System for being able to maintain that equilibrium state between oxidative and anti-oxidative, to slow down in human body
The generation of active oxygen and free radical, if but long-term excessive exposure in the sun, then the free radical meeting largely generated in human body
Lead to the anti-oxidative defense ability of skin to reduce, cause the injury of skin, such as light aging, creasy surface, the mistake of cutaneous immunisation power
Adjust etc..Antioxidant is with the substance for capturing free radical ability, can remove the living radical in skin, mitigates free radical
Caused skin oxidative stress damage, so as to improve the skin aging as caused by oxidative stress etc..Be currently known vitamin E,
Removing free radical type antioxidant in the organisms such as vitamin C, in addition also report plant origin antioxidant such as lotus flower,
Plum berry extract etc..
Skin aging is mainly two kinds of forms of naturally-aged and light aging, so-called naturally-aged (intrinsicaging) system
Mainly caused by inherent cause, hence it is evident that be characterized as the appearance of wrinkle and the relaxation of skin.And light aging (photoaging) then refers to
The accumulation of skin aging process ultraviolet damage is that natural aging and ultraviolet radiation are coefficient as a result, showing as skin
Exposure portion is rough, wrinkle deepens overstriking, scrambling pigmentation, blood vessel dilatation, keratinization of epidermis are bad, corium elastic fibers
Denaturation and catabolite accumulation etc..Cutis laxa, dry coarse, flexibility decrease, wrinkle increase etc. aging phenomena all with into fiber
Cell number is reduced and secretion complex functionality declines or abnormal related.Human skin fibroblasts be in dermis of skin reticular layer most
Important cell is one of main repair cell after skin aging and cell damage.It is not only able to promote epidermal cell
Migration, proliferation and differentiation, moreover it is possible to secrete a large amount of collagen, elastomer albumen and various kinds of cell reparative factor, have strong
Big self-renewal capacity repairs the skin of aging.
Saussurea involucrata is the civil common rare Chinese herbal medicine simply of China western part extremely frigid zones, is composite family phoenix hair Dendranthema, entirely
The world shares more than 40 kinds and 3 mutation, China share 12 kinds and 1 mutation with saussurea involucrata, is Herba Saussureae Involueratae Saussurea respectively
The sub- S. gossypiphora D Don of involucrate, snow hare, three refer to the sub- S. tridactyla Sch.-Bip. of snow hare
Ex Hook. f., sub- S. tridactyla Sch-Bip. var. maiduoganla S. W. Liu of clump strain snow hare, continuous head
Saussurea involucrata S. laniceps Hand.-Mazz., burr saussurea involucrata S. eriocephala Franch, Saussurea medusa S. medusa
Maxim., Mongolian oak leaf lanatechead saussurea herb with flower S. quercifolia W. W. Smith, mouse Qu saussurea involucrata S. gnaphaloides (Royle)
Sch. Bip, Saus-surea obvallata (DC.) Edgew S. obovallata (DC.) Sch. Bip, sub- S. stella Maxim. of starlike snow hare, fruitlet
The sub- S. simpsoniana of snow hare (Field. et Gardn.) Lipsc., snow hare S. leucoma Diels.Saussurea involucrata is main
It is grown between the rubble near the rock flowing hillside of high mountains and snow line of height above sea level 4800-5800 m, is mainly distributed on Xinjiang Tianshan, the Kunlun
One band of mountain, the Pamir Mountain Area and Altai Mountains.Saussurea involucrata applicating history is long, especially as medicinal, mainly contains flavones, sequiterpene
There is the biologies such as anti-oxidant, antirheumatic, analgesia, adjusting cardiovascular system, anticancer to live for lactone, cumarin, organic acid, polysaccharide etc.
Property.
Glacial water be in earth's surface long-term existence simultaneously can autonomous travel natural ice body.By air solid rainfall through accumulating for many years
It forms, is the important freshwater resources of earth's surface, the water freezed different from winter river and lake freezes.After new snow drops to ground, by one
The snow that a ablation season does not melt is granular snow.Snow is gradually closely knit, freezes again through melting, and merges crystal, crystal grain changes its size
And form, there is orientation and increase.When its density reaches critical value, intercrystalline pushes gas permeability and water penetration, becomes as glacier ice.
The scientist of many countries to often drink glacial water be conducive to improve human physique, reduce disease, extend the service life phenomena such as into
Go series of studies, and it was found that many important evidences, such as the characteristics of glacial water low deuterium, and low deuterium-oxide is exactly exactly the mankind
The healthy active water found.
In the prior art, has the report that the plant extracts that some saussurea involucrata belong to is used for skin preparations for extenal use.For example,
JP2004331547A describes a kind of high cosmetic composition of skin moisture-keeping performance, finds Saussurea involucrata extract and glycerine conduct
When moisturizer is used together, have synergistic effect, compared to be used alone when, performance of keeping humidity is significantly improved；
JP2002201122A, which describes Saussurea involucrata extract, has the effect for inhibiting tyrosinase activity and melanin being prevented to generate, and can make
It is added in cosmetics for whitening active substance；JP2004155961A describes purposes of the Saussurea involucrata extract as antioxidant；
CN102240254A, which describes Saussurea involucrata extract, has uva-resistant and UVB radiations effect.
But Extraction solvent is generally water or organic solvent used by the above prior art, obtaining Saussurea involucrata extract.
It is recorded wherein in the specification of JP2002201122A：When Extraction solvent is water, distilled water, tap water, underground may be used
Water, mineral water, thermal water, karst water, fresh water etc..However the prior art is made a general survey of, there is not yet using glacial water as Extraction solvent
The report of Saussurea involucrata extract is obtained, also has no the report that there are more preferably anti-aging effects with the Saussurea involucrata extract that glacial water extracts
Road.
Invention content
Technical problem solved by the invention is the provision of a kind of preparation method and its usage of Saussurea involucrata extract and contains
Its skin preparations for extenal use.
Saussurea involucrata employed in the present invention is composite family Genus Saussurea saussurea involucrata subgenus and the sub- Subgenus of snow hare, preferably Tianshan Mountains
Saussurea involucrata Saussurea involucrate and Saussurea laniceps flower Saussurea laniceps Hand.-Mazz..
In the present invention, the Saussurea involucrata extract for the root selected from saussurea involucrata, stem, leaf, spend in a kind or a kind passed through with upper bit
The extract that solvent extraction obtains（Hereinafter referred to as " Saussurea involucrata extract "）, the preferred flower of the present invention is as extract part.
The preparation method of Saussurea involucrata extract of the present invention, to improve extraction efficiency, preferably before extraction to extract part
The processing such as frittered, dried, being crushed, preferable particle diameter is 40-100 mesh after crushing.
The preparation method of Saussurea involucrata extract of the present invention is under room temperature, cooling or heating condition, by extract part
It is impregnated in the solvent extraction method of certain time in Extraction solvent.The Saussurea involucrata extract can be by any of the above-described extracting method system
, it can also be combined and be made by above-mentioned arbitrary extracting method of more than two kinds.To improve extraction efficiency, extraction process is preferably super
Sound extraction, stirring extraction or circulated in countercurrent extraction.Present invention the advantages of use solvent extraction method for, it is convenient to adjust extract it is molten
Agent, extraction time and Extracting temperature, for that, easily by the ingredient of environmental change, suitable conditional parameter can be taken to carry out in saussurea involucrata
Extraction.
The preparation method of heretofore described Saussurea involucrata extract, ranging from 5-50 DEG C of Extracting temperature；According to Extraction solvent
With the difference of Extracting temperature, extraction time, optional ranging from 5min was to for 24 hours.
In the present invention, selected solvent is glacial water, and the water head site of the glacial water can be any one location, preferably like
The refined mountain of horse traction.The preferably more lucky songs of the intake of the glacial water step on Nima spring.More Ji songs step on Nima spring and are located at Himalaya
Mountain north slope, 5128 meters of height above sea level, spring mouthful temperature are maintained at 1.5-2 DEG C throughout the year.
In the present invention, the glacial water preferably meets following conditions：The pH value of the glacial water be 7.1-7.9, institute
It is 133-137ppm to state the deuterium content in glacial water, and bromate is free of in the glacial water；More preferably, every liter of glacial water
Contain following components：Sr+ 0.2-0.5mg、K+ 0.5-10.0mg、Na+ 1.0-5.0mg、Ca2+14.0-30.0mg and Mg2+
2.0-10.0 mg；Total dissolved solid content in the glacial water is 60-150mg/L；Most preferably, the pH of the glacial water
It is 7.4 to be worth, and the deuterium content in the glacial water is 133ppm.
In the extraction process of the present invention, to the mass volume ratio of extract part and Extraction solvent, there is no particular limitation, right
In the flower position of saussurea involucrata, the mass volume ratio with solvent is preferably（1：5）G/mL~（1：20）g/mL.
The Saussurea involucrata extract of the present invention can be filtrate of each extract part of saussurea involucrata after the water extraction of glacier, and directly
It uses；Or be concentrating filter liquor, dried solid, it is used after being redissolved with water or polar solvent；Or do not damaging extraction
Under the premise of object active constituent, used after the separating treatments such as the process of refinement such as decolourized, be deodorized, chromatographic column absorption；Or
By it by being used after fatty vesicle or microemulsion package.
The Saussurea involucrata extract of the present invention is strong to the Scavenging activity of free radical, can be obviously promoted human skin fibroblasts
Proliferation activity, and natural, safety can be used as the activity of fighting against senium ingredient of skin preparations for extenal use.
In the present invention, the skin preparations for extenal use is typically used for the general designation concept of all the components outside skin, such as can
To be cosmetics or drug.Can be in the cosmetics basic cosmetics, facial dressing cosmetics, body apply some make up, head
Portion's nursing product etc. to its dosage form without specifically limited, can be reasonably selected according to different purposes.
In the present invention, weight percent of the Saussurea involucrata extract in skin preparations for extenal use is 0.0001-1, preferably 0.01-1.
In the present invention, the skin preparations for extenal use can also include other active components, and the other active components are preferable
Ground is in moisturizing active constituent, oxidation-resistant active ingredient, oil-control active constituent, whitening active ingredients and activity of fighting against senium ingredient
It is one or more.The content of the other active components is the content of this field routine.
Wherein, the moisturizing active constituent includes natural moisture preserving active constituent and/or synthesis moisturizing active constituent, described
Natural moisture preserving active constituent can be selected from：Saualane, jojoba oil, honey, lucidum extracting liquid, aloe extract, hyaluronic acid,
The natural materials such as ceramide, silk-fibroin class moisturizing active constituent, collagen；The synthesis moisturizing active constituent can select
From：Polyalcohols moisturizing ingredient（Glycerine, propylene glycol, butanediol, pentanediol, polyethylene glycol, polyglycereol etc.）, sodium lactate, grape
Sugar derivatives, polyacrylic resin, castor oil and its derivative, chitin chitosan and its derivative, pyrrolidone sodium carboxylate
Wait synthetics.
Wherein, the oxidation-resistant active ingredient can be selected from：Flavonoids oxidation-resistant active ingredient（Silymarin, Radix Astragali
Glycosides, rutin, Quercetin, green blue glycosides, the green blue glycosides of double hydrogen, mangiferin etc.）, tannin class oxidation-resistant active ingredient（Table does not have infanticide youngster
Theine, table do not have infanticide catechin and gallate, L-Epicatechin gallate, catechin etc.）, quinones antioxidant activity into
Point（Alkannin etc.）, dipeptides amino acid（Methionine, tryptophan, phenylalanine, proline etc.）, glycitols antioxidant activity into
Point（Pentose, hexose monosaccharide, lily polysaccharide, fructose, sugar alcohol etc.）, Polyphenols oxidation-resistant active ingredient（The plumule of cereal, Portugal
Grape phenol, rosmanol, table rosmanol, different rosmanol etc.）, alkaloids oxidation-resistant active ingredient（Rhizome of chuanxiong piperazine, jateorrhizine,
Magnoline, glaucine, Pan's isobebeerine etc.）, vitamins oxidation-resistant active ingredient（Vitamin E, vitamin C, carotenoid）
With the oxidation-resistant active ingredient of plant extract species, such as concave crown algae extract.
Wherein, the oil-control active constituent can be selected from：Astringent（Zinc oxide, zinc sulfate, aluminium chloride, aluminum sulfate, list
Peaceful acid, citric acid, lactic acid etc.）, freshener（Alcohol and peppermint）, vitamins oil-control active constituent（Vitamin B6, vitamin B3
And biotin）Lytic agent is removed with cutin（Salicylic acid, tartaric acid etc.）.
Wherein, the whitening active ingredients can be selected from：Ursin and its derivative, kojic acid and its derivative, nonyl two
Acid and its derivative, linoleic acid and its derivative, vitamin C and its derivative and tartaric acid.
Wherein, the activity of fighting against senium ingredient can be selected from：Vitamins activity of fighting against senium ingredient（Vitamin A, dimension life
Plain B）, enzyme（Superoxide dismutase SOD, glutathione peroxidase GPO, catalase etc.）And plant extracts（Caper
Bud extract, fragrant eupatorium herb extract etc.）.
According to common sense in the field, according to different dosage form and purpose, can also contain in the skin preparations for extenal use capable
Conventional use of medium or matrix excipients in domain, as long as the excipient is acceptable figuration in cosmetics or pharmaceutical field
Agent.The content of the excipient is the content of this field routine.
In the present invention, the form of the excipient can be water phase, oil phase, gel, water packet wax pattern lotion, oil-in-water type breast
The form of liquid or water-in-oil emulsion.The water phase can be the mixture of one or more water-soluble or dispersed components,
Can be liquid, semisolid or solid at room temperature.Those skilled in the art can be known based on what those skilled in the art grasped
Know the form for selecting suitable excipient and component wherein included.
When the form of the excipient is water phase, it is hydrophilic at least one that the excipient is preferably comprised water or water
The mixture of property organic solvent.The hydrophilic organic solvent such as alcohol, especially straight chain or branch containing 2-5 carbon atom
Chain low-grade monobasic alcohol, such as ethyl alcohol or propyl alcohol；Polyalcohol, such as propylene glycol, sorbierite, glycerine, panthenol or polyethylene glycol and its mixing
Object.
When the form of the excipient is lotion, the skin preparations for extenal use can also preferably include rheology modifiers
And/or film forming agent.The rheology modifiers can be selected from：Macromolecule thickener；The acrylate copolymer of hydrophobically modified, such as
Acrylate/Steareth-20 methyl acrylate copolymers, acrylate/Beheneth-25 acrylate copolymers；It is hydrophobic
Modified ethoxylated polyamines ester；Such as PEG-150/ decyl alcohol/SMDI copolymers, PEG-150/ stearyl alcohols/SMDI；Hydrophobically modified
Nonionic polyol, such as PEG-150 distearates.
The macromolecule thickener can be selected from：Cellulose thickener, agent for polyacrylic acid thickening, natural gum and its change
Property object, inorganic polymer and its modifier, polyoxyethylene thickener and other class thickeners；Wherein, cellulose thickener
It can be selected from：Cellulose, cellulose gum, carboxymethyl hydroxyethyl cellulose, spermaceti hydroxyethyl cellulose, ethyl cellulose, hydroxyl second
Base cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methylcellulose, carboxymethyl cellulose etc.；Polyacrylic increases
Thick dose can be selected from：Acrylate/C10-30Alkyl acrylate cross-linked polymer, acrylate/cetyl ethyoxyl（20）
Itaconate copolymeric, acrylate/cetyl ethyoxyl（20）Methacrylate copolymer, the acrylate/tetradecane
Base oxethyl（25）Acrylate copolymer, acrylate/octadecyl ethyoxyl（20）Itaconate copolymeric, acrylic acid
Ester/octadecyl ethyoxyl（20）Methacrylate copolymer, acrylate/octadecyl ethyoxyl（50）Acrylate is total to
Polymers, acrylate/VA cross-linked polymers, sodium acrylate/ethylene isodecyl acid esters cross-linked polymer, polyacrylic acid and its sodium salt
Deng；Natural gum and its modifier can be selected from：Alginic acid and its（Ammonium, calcium, potassium）Salt, pectin, Sodium Hyaluronate, guar gum, Huang
Virgin rubber, cation guar gum, hydroxypropyl guar gum, bassora gum, antler glue and its（Calcium, sodium）Salt, xanthan gum, sclerotium gum etc.；
Inorganic polymer and its modifier can be selected from：Aluminum magnesium silicate, silica, sodium magnesium silicate, hydrated SiO 2, montmorillonite,
Lithium metasilicate magnesium sodium, hectorite, stearic ammonium montmorillonite, stearic ammonium hectorite, -90 montmorillonite of quaternary ammonium salt, -18 montmorillonite of quaternary ammonium salt,
- 18 hectorite of quaternary ammonium salt etc.；Polyoxyethylene thickener can be selected from：PEG-n（n=5M、9M、23M、45M、90M、160M）Deng；
Other class thickeners can be selected from：The cross-linked polymer of polyvinyl methyl ethermaleic anhydride/methyl acrylate and decadinene, polyvinylpyrrolidine
Ketone etc..
The film forming agent can be film forming polymer commonly used in the art, as long as it dissolves in or be scattered in the figuration
In agent.The film forming agent be preferably it is following in it is one or more：Polyurethanes, polyacrylic acid homopolymer or copolymerization
Object, polyester, resin and/or silicone resin based on hydrocarbon.
When the form of the excipient is oil phase, the excipient is preferably comprised at room temperature as the oil-soluble of liquid
Or oil-dispersing property component and/or at room temperature be oily or wax-like substance.
It is described at room temperature for the oil-soluble of liquid or oil-dispersing property component be preferably comprised one kind in following substances or
It is a variety of：From the oil based on hydrocarbon of animal, such as perhydro-squalene；Vegetable oil based on hydrocarbon, such as the C4-10 aliphatic acid of liquid
Triglyceride, such as D3-C7 class, Trivent OCG class, Triglyceride DDD class or vegetable oil, for example, to
Day certain herbaceous plants with big flowers oil, corn oil, soybean oil, grape seed oil, castor oil, avocado oil, jojoba oil；The straight chain or branch of mineral or synthesis source
Catenanes, such as liquid paraffin and its derivant, vaseline；The esters of synthesizing ester and ethers, particularly fatty alcohol, such as meat
Isopropyl myristate, palmitic acid 2- ethylhexyls, stearic acid 2- octyldodecyls, isostearyl isostearate ester；Hydroxyl
Change esters, such as lactic acid isooctadecanol ester, octyl hydroxystearate, octyl hydroxystearate, hydroxy stearic acid octyldodecyl
Base ester, the enanthic acid esters of fatty alcohol, sad esters and capric acid lipid；Polyalcohol esters, such as propylene, new penta 2
Two heptanoate of alcohol, two isononoate of diethylene glycol (DEG) and pentaerythritol esters；Aliphatic alcohols containing C12-26, such as octyldodecyl
Alcohol, 2- butyl octanol, 2- hexyl decyl alcohols, 2- undecylpentadecanols, oleyl alcohol；Fluorocarbon oil and/or fluorosilicon oil based on part hydrocarbon,
Silicone oil is at room temperature liquid or semisolid volatility or non-volatile straight chain or cyclo-methicone, such as ring
Shape dimethyl silicone polymer and dimethyl silicone polymer, optionally comprising phenyl, such as Silicone DC 556, siloxanes and
Its mixture.
It is described to be preferably wax and/or natural gum for oily or wax-like substance at room temperature.The wax is preferably comprised
It is one or more in following substances：Wax based on hydrocarbon, fluorine wax and/or siloxane wax, and can derive from plant, mineral,
Animal and/or synthesis source；The wax more preferably includes one or more in following substances：It is beeswax, Brazil wax, small
Candle vegetable wax, paraffin, microwax, ceresine, polyethylene wax, the siloxane wax containing C16-45.The natural gum is generally poly- diformazan
Radical siloxane or sodium carboxymethylcellulose or polysaccharide, and semi-solid material is generally the compound based on hydrocarbon, such as lanolin
And its derivative.
The skin preparations for extenal use of the present invention can also further include conventional any other group being used in cosmetic field
Point, such as it is one or more in preservative, aromatic and hydrophily and lipophilic active agent.The content of those other components
It can be the content of this field routine.
In the present invention, the skin preparations for extenal use can also include particle phase, and the particle can be mutually cosmetic combinations
It is one or more in the pigment, pearling agent and the filler that are used in object.The content of the particle phase can be that this field is conventional
Content.The pigment is preferably inorganic pigment and/or organic pigment.The inorganic pigment is preferably comprised titanium oxide, oxidation
It is one or more in zirconium and cerium oxide, zinc oxide, iron oxide and barba hispanica.The organic pigment is preferably comprised barium color lake, strontium
Color lake, calcium color lake and aluminum lake and carbon black.The pearling agent is preferably the cloud for being coated with titanium oxide, iron oxide or natural pigment
It is female.The filler is preferably comprised talcum powder, silica, zinc stearate, mica, kaolin, nylon powder, polyethylene powder
It is one or more in end, Teflon, starch, borazon and copolymer microsphere.The copolymer microsphere is preferably silicon
Oxygen alkane resin microbeads.
The skin preparations for extenal use of the present invention can further include one or more following compositions：Anti-allergic agent resists micro- life
Agent, antioxidant, chelating agent, colorant depigmentation, emollient, emulsifier, excoriation agent, fragrance, moisturizer, insect
It is repellant, lubricant, pharmaceutically active agents, humidizer, photostabilizer, preservative, skin conditioner, skin penetration enhancer, sun-screening agent, steady
Determine agent, surfactant, thickener, viscosity modifier, vitamin or its arbitrary combination.The content of those ingredients can be this field
Conventional content.
In the present invention, the form of the skin preparations for extenal use can be any suitable product form, the product shape
Formula includes, but are not limited to aerosol spray, creme, lotion, solid, liquid, dispersion, foam, gel, toner, rubs
Silk, ointment, pulvis, patch, brilliantine, hand press pump spray, club, facial mask and hygenic towelette.It is it is also known that of the invention
Skin preparations for extenal use can be cosmetics, dermatology or drug local application product, preparation method can be that this field is conventional
Preparation method.
Specific embodiment
Saussurea involucrata extract prepared by 1 different solvents of embodiment, Extracting temperature, extraction time makees radicals scavenging
With comparing.
Herba Saussureae Involueratae and Saussurea laniceps is taken to spend each 10g, add ultra-pure water and glacial water respectively as Extraction solvent, setting extraction
Temperature is 5 DEG C, 15 DEG C, 25 DEG C and 35 DEG C, sets extraction time as 10min, 20min and 30min, takes filtrate, be evaporated, and is obtained each
The Saussurea involucrata extract of ultra-pure water and glacial water under a extraction conditions, it is spare.
The Saussurea involucrata extract prepared under each extraction conditions with deionized water is configured to the sample solution of 0.1mg/mL, is taken
Sample solution 2ml is added in test tube, adds in 76 mol/L DPPH ethanol solutions, 2 mL, and mixing is protected from light at dark at room temperature
30 min are reacted, absorbance (A525) is measured at 525 nm；Clearance rate, the bigger antioxygen of clearance rate are calculated according to formula below
Change ability is stronger, the results are shown in Table 1 and table 2.
Clearance rate I (%)=[1- (T-T0)/(C-C0)] × 100%
In formula： T0：0.1ml water adds the absorbance of 2 ml DPPH solution；
T：0.1ml sample liquids add the absorbance of 2 ml DPPH solution；
C0：0.1ml water adds the absorbance of 95% ethyl alcohol of 2ml；
C：0.1ml sample liquids add the absorbance of 95% ethyl alcohol of 2ml.
1 glacial water of table prepares Herba Saussureae Involueratae extract free radical scavenging ability（%, n=3）
2 glacial water of table prepares Saussurea laniceps flower extract free radical scavenging ability（%, n=3）
Temperature and extraction time are affected to Saussurea involucrata extract Scavenging ability, but ice it can be seen from Tables 1 and 2
Chuan Shui is strong compared with the Saussurea involucrata extract free radical scavenging ability that ultra-pure water is prepared under each extraction conditions, has better
Anti-aging effects.
2 different solvents of embodiment, the Saussurea involucrata extract prepared under Extracting temperature extraction time are to fibroblast proliferation shadow
It rings and compares.
The Saussurea involucrata extract prepared under 1 each extraction conditions of embodiment is added in human fibroblasts culture solution, with
Deionized water not with sample is blank control, tests final concentration of 0.1 mg/mL.After culture 48 hours, with mtt assay to cell
After dyeing, absorbance at 550nm is measured with microplate reader, the proliferation rate of blank control is 100%, and reference blank control is assessed to people
Fibroblastic proliferation function the results are shown in Table 3 and table 4.
3 glacial water of table prepares Herba Saussureae Involueratae extract to fibroblast proliferation facilitation（%, n=3）
4 glacial water of table prepares Saussurea laniceps flower extract to fibroblast proliferation facilitation（%, n=3）
The Saussurea involucrata extract pair that glacial water is prepared under each extraction conditions compared with ultra-pure water it can be seen from table 3 and table 4
Fibroblast proliferation facilitation is strong, and anti-aging effects are more notable.
General flavone content compares in 3 different solvents of embodiment, the Saussurea involucrata extract prepared under Extracting temperature extraction time.
Saussurea involucrata chemical composition includes flavonoids, Coumarins（Frutus cnidii lactone, bergapten, isoimpinellin, Ai get Er
Front yard etc.）, sterol（Alpha-amyrin, beta-amyrin, -3 β of ergot alkane, 24- glycol）, the Multiple components such as polysaccharide and volatile oil, wherein main
It is flavones and flavonoid glycoside compound to want secondary metabolite, such as apiolin, Kaempferol, Quercetin, Yi Quercetin skins glycosides, reseda
Element, rutin, apigenin-7-O-beta-D-lutinoside, apigenin-7-O-beta-D-new dried orange peel glucosides, cyanidenon -7-O- β-D- Portugals
Polyglycoside, cyanidenon -7-O- β-D- lutinosides, dinatin, acacetin, acacetin7OβDglucoside,
Eupatorin, chrysoeriol -7-O- β-D- lutinosides etc..
Saussurea laniceps is taken to spend each 10g, respectively plus ultra-pure water and glacial water are as Extraction solvent, set Extracting temperature as 5 DEG C,
15 DEG C, 25 DEG C, 35 DEG C and 45 DEG C, extraction time is set as 10min, 20min, 30min and 60min, filtrate is taken, is evaporated, obtain
The Saussurea involucrata extract of ultra-pure water and glacial water under each extraction conditions, it is spare.
Using tri-chlorination chlorine development process to the content of Flavonoid substances in Saussurea involucrata extract obtained under each extraction conditions
It measures using Quercetin as control, the results are shown in Table 5.
5 glacial water of table prepares Saussurea laniceps flower extract general flavone content（Mg/g, n=3）
As can be seen from Table 5, with the extension of extraction time and the raising of Extracting temperature, glacial water and ultra-pure water are obtained by solvent
To Saussurea involucrata extract in general flavone content in increase trend, and the Saussurea involucrata extract that glacial water is obtained for solvent is compared with ultra-pure water
It is high for general flavone content in the obtained Saussurea involucrata extract of solvent, illustrate more extract wherein by solvent of glacial water
Flavones ingredient, extraction efficiency is higher.
Toner of the embodiment 4 containing Saussurea involucrata extract
The Herba Saussureae Involueratae extract that glacial water is taken to prepare, is used to prepare the toner with anti-aging effects, the toner contains
Some components are as shown in table 6：
Table 6
Essence of the embodiment 5 containing Saussurea involucrata extract
The Saussurea laniceps flower extract that glacial water is taken to prepare, is used to prepare the Essence with anti-aging effects, the Essence
The component contained is as shown in table 7：
Table 7
Lotion/frost of the embodiment 7 containing Saussurea involucrata extract
The Herba Saussureae Involueratae extract that glacial water is taken to prepare, is used to prepare lotion/frost with anti-aging effects, the lotion/frost
The component contained is as shown in table 8：
Table 8
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
All any modification, equivalent and improvement made within principle etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of Saussurea involucrata extract, which is characterized in that by the root of saussurea involucrata, stem, leaf, spend in a kind or a kind with upper bit through containing
The solvent extraction of glacial water and obtain.
2. Saussurea involucrata extract as described in claim 1, which is characterized in that in the solvent containing glacial water, glacial water
A concentration of 10-100v/v%.
3. Saussurea involucrata extract as claimed in claim 2, which is characterized in that the pH value of the glacial water be 7.1-7.9, the ice
Deuterium content in the water of river is 133-137ppm, and bromate is free of in the glacial water.
4. Saussurea involucrata extract as claimed in claim 3, which is characterized in that every liter of glacial water contains following components：Sr+
0.2-0.5mg、K+ 0.5-10.0mg、Na+ 1.0-5.0mg、Ca2+14.0-30.0mg and Mg2+2.0-10.0mg；It is described
Total dissolved solid content in glacial water is 60-150mg/L.
5. such as claim 1-4 any one of them Saussurea involucrata extracts, which is characterized in that the Saussurea involucrata extract is by following preparations
Method is made：Take the root of saussurea involucrata, stem, leaf, spend in a kind or a kind with upper bit, be crushed to coarse powder, the particle of the coarse powder is straight
Diameter is 40-100 mesh, is added in the solvent containing glacial water, and 5min is leached at a temperature of 5-50 DEG C to for 24 hours, filtrate is collected, steams
It is dry, obtain Saussurea involucrata extract；
Wherein described saussurea involucrata position and the mass volume ratio of the solvent containing glacial water are（1：5）G/mL~（1：20）g/mL.
6. Saussurea involucrata extract as claimed in claim 5, which is characterized in that the Extracting temperature is 5-15 DEG C.
7. Saussurea involucrata extract as claimed in claim 5, which is characterized in that the time of the leaching is 10-60min.
8. the purposes of the Saussurea involucrata extract as described in any one of claim 1-4, which is characterized in that the Saussurea involucrata extract is made
For the activity of fighting against senium ingredient in skin preparations for extenal use；Wherein, weight percent of the Saussurea involucrata extract in skin preparations for extenal use
It is 0.0001-1, preferably 0.01-1.
9. purposes as claimed in claim 8, which is characterized in that the activity of fighting against senium ingredient is with radical scavenging activity
The activity of fighting against senium ingredient of power.
10. purposes as claimed in claim 8, which is characterized in that the activity of fighting against senium ingredient is with promoting into fiber
The activity of fighting against senium ingredient of cell-proliferation activity.
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| CN112791003A (en) * | 2021-01-26 | 2021-05-14 | 上海澄穆生物科技有限公司 | Use of retinoic acid receptor agonist compounds in cosmetic compositions |
| CN113599320A (en) * | 2021-08-09 | 2021-11-05 | 珠海金肽生物科技有限公司 | Composition for culturing pluripotent cells, preparation method and application |
| CN115400050A (en) * | 2021-05-28 | 2022-11-29 | 株式会社新世界国际 | Antiaging composition containing extracts of herba Saussureae Involueratae, usnea and fermented radix scrophulariae |
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| CN115400050A (en) * | 2021-05-28 | 2022-11-29 | 株式会社新世界国际 | Antiaging composition containing extracts of herba Saussureae Involueratae, usnea and fermented radix scrophulariae |
| CN113599320A (en) * | 2021-08-09 | 2021-11-05 | 珠海金肽生物科技有限公司 | Composition for culturing pluripotent cells, preparation method and application |
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