CN108235708A - 扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 - Google Patents
扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 Download PDFInfo
- Publication number
- CN108235708A CN108235708A CN201880000168.9A CN201880000168A CN108235708A CN 108235708 A CN108235708 A CN 108235708A CN 201880000168 A CN201880000168 A CN 201880000168A CN 108235708 A CN108235708 A CN 108235708A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- jnk
- candidate stem
- cells
- progenitor cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (13)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711486197 | 2017-12-29 | ||
CN2017114861974 | 2017-12-29 | ||
PCT/CN2018/072961 WO2019127697A1 (zh) | 2017-12-29 | 2018-01-17 | 扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108235708A true CN108235708A (zh) | 2018-06-29 |
CN108235708B CN108235708B (zh) | 2020-12-01 |
Family
ID=62658016
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201880000168.9A Active CN108235708B (zh) | 2017-12-29 | 2018-01-17 | 扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108235708B (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110564687A (zh) * | 2019-11-05 | 2019-12-13 | 诺未科技(北京)有限公司 | 用于扩增造血干细胞的组合物、培养基、方法和试剂盒 |
CN110564686A (zh) * | 2019-11-05 | 2019-12-13 | 诺未科技(北京)有限公司 | 扩增造血干细胞的组合物、扩增方法、药物组合物和用途 |
CN110669732A (zh) * | 2019-11-05 | 2020-01-10 | 清华大学 | 组合物在将造血祖细胞重编程为造血干细胞中的用途 |
CN110904041A (zh) * | 2019-12-05 | 2020-03-24 | 中山大学 | 一种促进造血干细胞扩增的药物及其用途 |
CN110938597A (zh) * | 2018-09-25 | 2020-03-31 | 北京大学 | 一种扩增造血干细胞的方法及其应用 |
CN112048474A (zh) * | 2020-09-21 | 2020-12-08 | 海南苏生生物科技有限公司 | 一种增强造血干细胞移植能力的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1668733A (zh) * | 2002-05-30 | 2005-09-14 | 细胞基因公司 | 利用jnk或mkk抑制剂调节细胞分化并治疗骨髓增生异常和脊髓发育不良综合症的方法 |
US20070036765A1 (en) * | 2003-02-12 | 2007-02-15 | Civin Curt I | Therapeutic and diagnostic applications of genes differentially expressed in lympho-hematopoietic stem cells |
CN104693075A (zh) * | 2015-02-13 | 2015-06-10 | 程涛 | p18小分子抑制剂及在人造血干细胞体外扩增中的用途 |
CN105296427A (zh) * | 2015-10-23 | 2016-02-03 | 深圳爱生再生医学科技有限公司 | 造血干细胞的体外扩增培养方法 |
WO2016016894A1 (en) * | 2014-07-30 | 2016-02-04 | Yeda Research And Development Co. Ltd. | Media for culturing pluripotent stem cells |
CN105441384A (zh) * | 2014-09-26 | 2016-03-30 | 北京大学 | 一种制备动物和人类原始多潜能干细胞的方法,试剂盒及用途 |
-
2018
- 2018-01-17 CN CN201880000168.9A patent/CN108235708B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1668733A (zh) * | 2002-05-30 | 2005-09-14 | 细胞基因公司 | 利用jnk或mkk抑制剂调节细胞分化并治疗骨髓增生异常和脊髓发育不良综合症的方法 |
US20070036765A1 (en) * | 2003-02-12 | 2007-02-15 | Civin Curt I | Therapeutic and diagnostic applications of genes differentially expressed in lympho-hematopoietic stem cells |
WO2016016894A1 (en) * | 2014-07-30 | 2016-02-04 | Yeda Research And Development Co. Ltd. | Media for culturing pluripotent stem cells |
CN105441384A (zh) * | 2014-09-26 | 2016-03-30 | 北京大学 | 一种制备动物和人类原始多潜能干细胞的方法,试剂盒及用途 |
CN104693075A (zh) * | 2015-02-13 | 2015-06-10 | 程涛 | p18小分子抑制剂及在人造血干细胞体外扩增中的用途 |
CN105296427A (zh) * | 2015-10-23 | 2016-02-03 | 深圳爱生再生医学科技有限公司 | 造血干细胞的体外扩增培养方法 |
Non-Patent Citations (4)
Title |
---|
CHRISTIAN R. GEEST 等: "MAPK signaling pathways in the regulation of hematopoiesis", 《JOURNAL OF LEUKOCYTE BIOLOGY》 * |
MARIANNE SANTAGUIDA 等: "JunB Protects against Myeloid Malignancies by Limiting Hematopoietic Stem Cell Proliferation and Differentiation without Affecting Self-Renewal", 《CANCER CELL》 * |
TINGHU ZHANG 等: "Discovery of Potent and Selective Covalent Inhibitors of JNK", 《CHEMISTRY & BIOLOGY》 * |
余元勋 等: "《中国疾病信号通路与靶向治疗学》", 30 May 2013, 安徽科学技术出版社 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110938597A (zh) * | 2018-09-25 | 2020-03-31 | 北京大学 | 一种扩增造血干细胞的方法及其应用 |
CN110564687A (zh) * | 2019-11-05 | 2019-12-13 | 诺未科技(北京)有限公司 | 用于扩增造血干细胞的组合物、培养基、方法和试剂盒 |
CN110564686A (zh) * | 2019-11-05 | 2019-12-13 | 诺未科技(北京)有限公司 | 扩增造血干细胞的组合物、扩增方法、药物组合物和用途 |
CN110669732A (zh) * | 2019-11-05 | 2020-01-10 | 清华大学 | 组合物在将造血祖细胞重编程为造血干细胞中的用途 |
CN110564686B (zh) * | 2019-11-05 | 2020-04-03 | 诺未科技(北京)有限公司 | 扩增造血干细胞的组合物、扩增方法、药物组合物和用途 |
CN110564687B (zh) * | 2019-11-05 | 2020-04-03 | 诺未科技(北京)有限公司 | 用于扩增造血干细胞的组合物、培养基、方法和试剂盒 |
WO2021088294A1 (zh) * | 2019-11-05 | 2021-05-14 | 诺未科技(北京)有限公司 | 用于扩增造血干细胞的组合物、培养基、方法和试剂盒 |
WO2021088293A1 (zh) * | 2019-11-05 | 2021-05-14 | 诺未科技(北京)有限公司 | 扩增造血干细胞的组合物、扩增方法、药物组合物和用途 |
CN110904041A (zh) * | 2019-12-05 | 2020-03-24 | 中山大学 | 一种促进造血干细胞扩增的药物及其用途 |
CN112048474A (zh) * | 2020-09-21 | 2020-12-08 | 海南苏生生物科技有限公司 | 一种增强造血干细胞移植能力的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN108235708B (zh) | 2020-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108235708A (zh) | 扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 | |
Sensebé et al. | Production of mesenchymal stromal/stem cells according to good manufacturing practices: a review | |
Ejtehadifar et al. | The effect of hypoxia on mesenchymal stem cell biology | |
Latchney et al. | The aging hematopoietic stem cell niche: Phenotypic and functional changes and mechanisms that contribute to hematopoietic aging | |
Chen et al. | Mitochondrial bioenergetic function and metabolic plasticity in stem cell differentiation and cellular reprogramming | |
CN105112362B (zh) | 一种胎盘间充质干细胞的无血清培养基及其制备方法 | |
Mizukami et al. | A fully-closed and automated hollow fiber bioreactor for clinical-grade manufacturing of human mesenchymal stem/stromal cells | |
Mizukami et al. | Efficient expansion of mesenchymal stromal cells in a disposable fixed bed culture system | |
CN105062957A (zh) | 血管内皮细胞祖细胞的培养方法 | |
CN103451153A (zh) | 一种临床级神经干细胞系的建立方法及用途 | |
CN104928230B (zh) | 血管内皮细胞的培养方法 | |
CN104830763A (zh) | Y-27632在间充质干细胞培养中的应用及间充质干细胞的培养方法 | |
Huang et al. | Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation | |
Di Mattia et al. | Insight into hypoxia stemness control | |
Gurung et al. | Comparing the effect of TGF-β receptor inhibition on human perivascular mesenchymal stromal cells derived from endometrium, bone marrow and adipose tissues | |
CN108456658A (zh) | 促进造血干细胞扩增并抑制其分化的化合物及细胞因子组合物 | |
WO2019127697A1 (zh) | 扩增造血干细胞和/或造血祖细胞的培养体系及其方法、造血干细胞以及造血祖细胞 | |
Branco et al. | Hypothermic Preservation of Adipose-Derived Mesenchymal Stromal Cells as a Viable Solution for the Storage and Distribution of Cell Therapy Products | |
Ross et al. | Nanotopography reveals metabolites that maintain the immunosuppressive phenotype of mesenchymal stem cells | |
Eaves et al. | Characterization of human hematopoietic cells with short‐lived in vivo repopulating activity | |
CN103194428A (zh) | 一种胰岛素样生长因子联合多种细胞因子体外分化并扩增人天然杀伤细胞的培养方法 | |
Pan et al. | Organoid models of gastrointestinal Neoplasms: Origin, current status and future applications in personalized medicine | |
CN104928244B (zh) | 细胞培养基及其制备方法和应用 | |
Reems | A journey to produce platelets in vitro | |
Wuchter et al. | Mesenchymal stem cells: an oversimplified nomenclature for extremely heterogeneous progenitors |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20181102 Address after: 100176 room 103, level B, seat 18, West Ring Road, Yizhuang Economic Development Zone, Beijing. Applicant after: Connaught Technology (Beijing) Co., Ltd. Applicant after: Beijing Union Pharmaceutical Science and Technology Co Ltd Address before: 100081 Minzu University of China, 27 Zhongguancun South Street, Haidian District, Minzu University of China Applicant before: Luo Xiaoxia |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20200526 Address after: 100176 room 103, floor 1, building B, No. 18, Xihuan South Road, Yizhuang Economic Development Zone, Daxing District, Beijing Applicant after: Nuowei Technology (Beijing) Co., Ltd Address before: 100176 room 103, level B, seat 18, West Ring Road, Yizhuang Economic Development Zone, Beijing. Applicant before: Nuowei Technology (Beijing) Co., Ltd Applicant before: BEIJING XIEKE PHARMACEUTICAL TECHNOLOGY Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant |