CN108220179A - A kind of culture medium for fermentative lactobacillus - Google Patents
A kind of culture medium for fermentative lactobacillus Download PDFInfo
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- CN108220179A CN108220179A CN201611145671.2A CN201611145671A CN108220179A CN 108220179 A CN108220179 A CN 108220179A CN 201611145671 A CN201611145671 A CN 201611145671A CN 108220179 A CN108220179 A CN 108220179A
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- triangular flask
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- yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention is a kind of culture medium for fermentative lactobacillus, and raw material proportioning is following (g/L)：Corn flour 3.3 4.4, tapioca flour 1.2 2.0, beef extract 5 10, yeast extract 58, dusty yeast 1.2 1.8, ammonium citrate 25, potassium hydrogen phosphate 1.5 2.0,1 1.5 glucose 2.2 3.0 of Pig cholate, lactose 2.3 2.5, agar 25 30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C；By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will pour into triangular flask after raw material proportioning, with the water dissolution of 100ml, then shake up, is sterilized triangular flask is placed in sterilization box；After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high saccharomycete of production activity.The culture medium of the present invention prepares fairly simple, and culture medium nutrition is more abundant, the growth characteristics of entirely appropriate lactic acid bacteria.
Description
Technical field
The present invention relates to saccharomycete culture technique field, specifically a kind of culture mediums for fermentative lactobacillus.
Background technology
Lactic acid bacteria is the main bacteria seed of fermentation, and Spawn incubation obtains good ring, directly influences the quality of fermentation.Therefore, it carries out
Cultivation work is very important, however present culture medium is chemical solution mostly, pollutes environment, is had after culture chemical residual
It stays, influences strain development.
Invention content
The present invention provides a kind of culture medium for fermentative lactobacillus.
The technical solution adopted by the present invention is：
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-10, yeast extract 5-8,
Dusty yeast 1.2-1.8, ammonium citrate 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-
2.5, agar 25-30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, is used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
It is an advantage of the invention that：The culture medium of the present invention prepares fairly simple, and culture medium nutrition is more abundant, entirely appropriate
The growth characteristics of lactic acid bacteria.
Specific embodiment
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-10, yeast extract 5-8,
Dusty yeast 1.2-1.8, ammonium citrate 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-
2.5, agar 25-30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, is used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
Claims (1)
1. a kind of culture medium for fermentative lactobacillus, it is characterised in that：
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-10, yeast extract 5-8, yeast
Powder 1.2-1.8, ammonium citrate 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, fine jade
Fat 25-30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, with 100ml's
Then water dissolution shakes up, sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin and life
The long factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high yeast of production activity
Bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611145671.2A CN108220179A (en) | 2016-12-13 | 2016-12-13 | A kind of culture medium for fermentative lactobacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611145671.2A CN108220179A (en) | 2016-12-13 | 2016-12-13 | A kind of culture medium for fermentative lactobacillus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108220179A true CN108220179A (en) | 2018-06-29 |
Family
ID=62638038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611145671.2A Pending CN108220179A (en) | 2016-12-13 | 2016-12-13 | A kind of culture medium for fermentative lactobacillus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108220179A (en) |
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2016
- 2016-12-13 CN CN201611145671.2A patent/CN108220179A/en active Pending
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180629 |